AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46956


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46956.t1 MTR_3g449790 91.304 69 6 0 28 96 292 360 1.24e-37 129
MS.gene46956.t1 MTR_3g449790 91.304 69 6 0 28 96 298 366 1.35e-37 129
MS.gene46956.t1 MTR_3g092140 37.349 83 47 2 2 84 274 351 9.12e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46956.t1 AT1G06690 69.565 69 21 0 28 96 309 377 1.15e-28 106

Find 19 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGAAAATTAAGCCTGTTAT+TGG 0.217835 3.2:-37628825 MS.gene46956:CDS
TCAACTGGAAAGCCAATAAC+AGG 0.332654 3.2:+37628813 None:intergenic
GAACAAGCTGAAGAATTTAA+AGG 0.335134 3.2:-37628903 MS.gene46956:CDS
GGTAGCTGAGCTACGAAGTT+TGG 0.341379 3.2:-37628850 MS.gene46956:CDS
TTTGTTAGGTATCACTAAAC+TGG 0.342680 3.2:-37628974 MS.gene46956:intron
TTATATCGCCATGTGGTCAT+TGG 0.400355 3.2:-37629419 MS.gene46956:CDS
GGAAATGTTGTACCAATTCC+TGG 0.415471 3.2:-37628939 MS.gene46956:CDS
GAAGAATTTAAAGGTGCATT+GGG 0.420507 3.2:-37628894 MS.gene46956:CDS
TGAAGAATTTAAAGGTGCAT+TGG 0.443008 3.2:-37628895 MS.gene46956:CDS
AATTTAAAGGTGCATTGGGA+TGG 0.525566 3.2:-37628890 MS.gene46956:CDS
TTAGGTATCACTAAACTGGC+TGG 0.543245 3.2:-37628970 MS.gene46956:intron
CTAAACTGGCTGGTAGCTCA+AGG 0.571830 3.2:-37628960 MS.gene46956:CDS
ATGGAGATTGACTAATGAAG+AGG 0.577740 3.2:-37628871 MS.gene46956:CDS
AATTCGCTCAATTTGAACGT+GGG 0.581250 3.2:+37629353 None:intergenic
CAATTAAGCCAATGACCACA+TGG 0.625087 3.2:+37629411 None:intergenic
ATTCGCTCAATTTGAACGTG+GGG 0.640247 3.2:+37629354 None:intergenic
TAATTCGCTCAATTTGAACG+TGG 0.645872 3.2:+37629352 None:intergenic
ACAATAGTGATATTAGTGAG+AGG 0.660892 3.2:+37629381 None:intergenic
CATGGTGTTATATCGCCATG+TGG 0.736577 3.2:-37629426 MS.gene46956:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGAATCAGTTTGTTTTTA+AGG + chr3.2:37629237-37629256 None:intergenic 20.0%
!!! AAATATTTTTGGTATTCTCA+TGG - chr3.2:37628959-37628978 MS.gene46956:CDS 20.0%
!!! AAGAATCAGTTTGTTTTTAA+GGG + chr3.2:37629236-37629255 None:intergenic 20.0%
!!! AATATTTTTGGTATTCTCAT+GGG - chr3.2:37628960-37628979 MS.gene46956:CDS 20.0%
!!! GTTGTCTAAGTAAATATTTT+TGG - chr3.2:37628948-37628967 MS.gene46956:CDS 20.0%
! ACACAAGTATTATTATCTGA+AGG + chr3.2:37629208-37629227 None:intergenic 25.0%
! GAGACTTAAATGTAATAAGA+GGG + chr3.2:37628919-37628938 None:intergenic 25.0%
! GTGTTACAATTGTGTAATTT+CGG + chr3.2:37629144-37629163 None:intergenic 25.0%
! TGAGACTTAAATGTAATAAG+AGG + chr3.2:37628920-37628939 None:intergenic 25.0%
!! AAACAAACTGATTCTTTGTT+AGG - chr3.2:37629240-37629259 MS.gene46956:intron 25.0%
!!! ATATTTTTGGTATTCTCATG+GGG - chr3.2:37628961-37628980 MS.gene46956:CDS 25.0%
AATAAGAGGGTTACTTACTT+TGG + chr3.2:37628906-37628925 None:intergenic 30.0%
ACAATAGTGATATTAGTGAG+AGG + chr3.2:37628850-37628869 None:intergenic 30.0%
GAACAAGCTGAAGAATTTAA+AGG - chr3.2:37629325-37629344 MS.gene46956:intron 30.0%
TCGAAAATTAAGCCTGTTAT+TGG - chr3.2:37629403-37629422 MS.gene46956:CDS 30.0%
TTTGTTAGGTATCACTAAAC+TGG - chr3.2:37629254-37629273 MS.gene46956:intron 30.0%
! AATTGCACTTTTAGTCTATC+AGG + chr3.2:37629166-37629185 None:intergenic 30.0%
! GAAGAATTTAAAGGTGCATT+GGG - chr3.2:37629334-37629353 MS.gene46956:intron 30.0%
! TGAAGAATTTAAAGGTGCAT+TGG - chr3.2:37629333-37629352 MS.gene46956:intron 30.0%
!!! TATTTTTGGTATTCTCATGG+GGG - chr3.2:37628962-37628981 MS.gene46956:CDS 30.0%
AATTCGCTCAATTTGAACGT+GGG + chr3.2:37628878-37628897 None:intergenic 35.0%
ATGGAGATTGACTAATGAAG+AGG - chr3.2:37629357-37629376 MS.gene46956:CDS 35.0%
TAATTCGCTCAATTTGAACG+TGG + chr3.2:37628879-37628898 None:intergenic 35.0%
TGTAACAAGATGCATGCTTA+GGG - chr3.2:37629088-37629107 MS.gene46956:intron 35.0%
TTGTAACAAGATGCATGCTT+AGG - chr3.2:37629087-37629106 MS.gene46956:intron 35.0%
! AATTTAAAGGTGCATTGGGA+TGG - chr3.2:37629338-37629357 MS.gene46956:intron 35.0%
! TAATTCCTGCATTTGAAACC+TGG - chr3.2:37629114-37629133 MS.gene46956:intron 35.0%
ACAATCAAGTCCGTTGTCAT+TGG + chr3.2:37629071-37629090 None:intergenic 40.0%
ATTCGCTCAATTTGAACGTG+GGG + chr3.2:37628877-37628896 None:intergenic 40.0%
CAATTAAGCCAATGACCACA+TGG + chr3.2:37628820-37628839 None:intergenic 40.0%
GGAAATGTTGTACCAATTCC+TGG - chr3.2:37629289-37629308 MS.gene46956:intron 40.0%
TCAACTGGAAAGCCAATAAC+AGG + chr3.2:37629418-37629437 None:intergenic 40.0%
TGCAGCACATGCACTAATAA+TGG + chr3.2:37629008-37629027 None:intergenic 40.0%
TTAGGTATCACTAAACTGGC+TGG - chr3.2:37629258-37629277 MS.gene46956:intron 40.0%
TTATATCGCCATGTGGTCAT+TGG - chr3.2:37628809-37628828 MS.gene46956:CDS 40.0%
TTGTGTAATTTCGGTTCTCC+AGG + chr3.2:37629135-37629154 None:intergenic 40.0%
GTATTCTCATGGGGGCAAAA+AGG - chr3.2:37628970-37628989 MS.gene46956:intron 45.0%
GTCGACAATGCCAATGACAA+CGG - chr3.2:37629058-37629077 MS.gene46956:intron 45.0%
GTTCTCCAGGTTTCAAATGC+AGG + chr3.2:37629122-37629141 None:intergenic 45.0%
GTTGTCATTGGCATTGTCGA+CGG + chr3.2:37629059-37629078 None:intergenic 45.0%
!!! GCTGTTTTAGCACCAGGAAT+TGG + chr3.2:37629304-37629323 None:intergenic 45.0%
!!! TGTTCTGCTGTTTTAGCACC+AGG + chr3.2:37629310-37629329 None:intergenic 45.0%
CTAAACTGGCTGGTAGCTCA+AGG - chr3.2:37629268-37629287 MS.gene46956:intron 50.0%
GCATTGTCGACGGTTCACAA+GGG + chr3.2:37629049-37629068 None:intergenic 50.0%
GGTAGCTGAGCTACGAAGTT+TGG - chr3.2:37629378-37629397 MS.gene46956:CDS 50.0%
GGCATTGTCGACGGTTCACA+AGG + chr3.2:37629050-37629069 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 37628803 37629447 37628803 ID=MS.gene46956
chr3.2 mRNA 37628803 37629447 37628803 ID=MS.gene46956.t1;Parent=MS.gene46956
chr3.2 exon 37629343 37629447 37629343 ID=MS.gene46956.t1.exon1;Parent=MS.gene46956.t1
chr3.2 CDS 37629343 37629447 37629343 ID=cds.MS.gene46956.t1;Parent=MS.gene46956.t1
chr3.2 exon 37628803 37628988 37628803 ID=MS.gene46956.t1.exon2;Parent=MS.gene46956.t1
chr3.2 CDS 37628803 37628988 37628803 ID=cds.MS.gene46956.t1;Parent=MS.gene46956.t1
Gene Sequence

>MS.gene46956

ATGGTGTTATATCGCCATGTGGTCATTGGCTTAATTGCACTTTTCCTCTCACTAATATCACTATTGTGCGTCCCCACGTTCAAATTGAGCGAATTAAGTTCCAAAGTAAGTAACCCTCTTATTACATTTAAGTCTCACTTATTGTGTTGTCTAAGTAAATATTTTTGGTATTCTCATGGGGGCAAAAAGGAGACAACATCAGCCATTATTAGTGCATGTGCTGCACTGCACGTGAAAATTCGTCCCTTGTGAACCGTCGACAATGCCAATGACAACGGACTTGATTGTAACAAGATGCATGCTTAGGGATTTAATTCCTGCATTTGAAACCTGGAGAACCGAAATTACACAATTGTAACACCTGATAGACTAAAAGTGCAATTAAGCTTTTAATTAGAATAGCCTTCAGATAATAATACTTGTGTGTTATCCCTTAAAAACAAACTGATTCTTTGTTAGGTATCACTAAACTGGCTGGTAGCTCAAGGAAATGTTGTACCAATTCCTGGTGCTAAAACAGCAGAACAAGCTGAAGAATTTAAAGGTGCATTGGGATGGAGATTGACTAATGAAGAGGTAGCTGAGCTACGAAGTTTGGCGTCGAAAATTAAGCCTGTTATTGGCTTTCCAGTTGAAAAACTCTGA

Protein sequence

>MS.gene46956.t1

MVLYRHVVIGLIALFLSLISLLCVPTFKLSELSSKVSLNWLVAQGNVVPIPGAKTAEQAEEFKGALGWRLTNEEVAELRSLASKIKPVIGFPVEKL