Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36504.t1 | XP_013457360.1 | 99.1 | 330 | 2 | 1 | 1 | 330 | 459 | 787 | 2.50E-182 | 647.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36504.t1 | Q9SA05 | 70.6 | 326 | 89 | 3 | 9 | 330 | 474 | 796 | 1.6e-129 | 463.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36504.t1 | A0A072UPX3 | 99.1 | 330 | 2 | 1 | 1 | 330 | 459 | 787 | 1.8e-182 | 647.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene36504 | 0.808486 | 3.03E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene36504 | 0.823189 | 1.58E-53 | -1.69E-46 |
| MS.gene049369 | MS.gene36504 | 0.830052 | 3.65E-55 | -1.69E-46 |
| MS.gene049427 | MS.gene36504 | 0.812733 | 3.65E-51 | -1.69E-46 |
| MS.gene049488 | MS.gene36504 | 0.812501 | 4.11E-51 | -1.69E-46 |
| MS.gene049491 | MS.gene36504 | 0.820535 | 6.51E-53 | -1.69E-46 |
| MS.gene049661 | MS.gene36504 | 0.804895 | 1.73E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene36504 | 0.816098 | 6.59E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene36504 | 0.800448 | 1.43E-48 | -1.69E-46 |
| MS.gene050397 | MS.gene36504 | 0.837955 | 3.83E-57 | -1.69E-46 |
| MS.gene050399 | MS.gene36504 | 0.801377 | 9.27E-49 | -1.69E-46 |
| MS.gene050400 | MS.gene36504 | 0.803695 | 3.08E-49 | -1.69E-46 |
| MS.gene050529 | MS.gene36504 | 0.819423 | 1.17E-52 | -1.69E-46 |
| MS.gene050593 | MS.gene36504 | 0.805023 | 1.63E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene36504 | 0.810074 | 1.38E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene36504 | 0.85015 | 2.05E-60 | -1.69E-46 |
| MS.gene05105 | MS.gene36504 | 0.828513 | 8.62E-55 | -1.69E-46 |
| MS.gene051255 | MS.gene36504 | 0.801118 | 1.05E-48 | -1.69E-46 |
| MS.gene051360 | MS.gene36504 | 0.82979 | 4.23E-55 | -1.69E-46 |
| MS.gene051666 | MS.gene36504 | 0.802796 | 4.73E-49 | -1.69E-46 |
| MS.gene051692 | MS.gene36504 | 0.837062 | 6.49E-57 | -1.69E-46 |
| MS.gene051858 | MS.gene36504 | 0.809151 | 2.18E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene36504 | 0.803408 | 3.54E-49 | -1.69E-46 |
| MS.gene052078 | MS.gene36504 | 0.801977 | 6.98E-49 | -1.69E-46 |
| MS.gene052136 | MS.gene36504 | 0.806427 | 8.27E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene36504 | 0.83858 | 2.64E-57 | -1.69E-46 |
| MS.gene052253 | MS.gene36504 | 0.834544 | 2.82E-56 | -1.69E-46 |
| MS.gene052310 | MS.gene36504 | 0.811851 | 5.69E-51 | -1.69E-46 |
| MS.gene052322 | MS.gene36504 | 0.809377 | 1.95E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene36504 | 0.834032 | 3.79E-56 | -1.69E-46 |
| MS.gene052414 | MS.gene36504 | 0.829826 | 4.14E-55 | -1.69E-46 |
| MS.gene052481 | MS.gene36504 | 0.852714 | 3.85E-61 | -1.69E-46 |
| MS.gene052484 | MS.gene36504 | 0.856164 | 3.87E-62 | -1.69E-46 |
| MS.gene052485 | MS.gene36504 | 0.836276 | 1.03E-56 | -1.69E-46 |
| MS.gene053103 | MS.gene36504 | 0.81614 | 6.45E-52 | -1.69E-46 |
| MS.gene053104 | MS.gene36504 | 0.811743 | 6.01E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene36504 | 0.839378 | 1.64E-57 | -1.69E-46 |
| MS.gene053449 | MS.gene36504 | 0.859217 | 4.82E-63 | -1.69E-46 |
| MS.gene053557 | MS.gene36504 | 0.800314 | 1.53E-48 | -1.69E-46 |
| MS.gene053758 | MS.gene36504 | 0.841932 | 3.52E-58 | -1.69E-46 |
| MS.gene053912 | MS.gene36504 | 0.821425 | 4.07E-53 | -1.69E-46 |
| MS.gene05505 | MS.gene36504 | 0.805609 | 1.23E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene36504 | 0.818378 | 2.02E-52 | -1.69E-46 |
| MS.gene055206 | MS.gene36504 | 0.806656 | 7.40E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene36504 | 0.822651 | 2.11E-53 | -1.69E-46 |
| MS.gene055722 | MS.gene36504 | 0.817546 | 3.12E-52 | -1.69E-46 |
| MS.gene056191 | MS.gene36504 | 0.827114 | 1.87E-54 | -1.69E-46 |
| MS.gene056410 | MS.gene36504 | 0.824605 | 7.37E-54 | -1.69E-46 |
| MS.gene056422 | MS.gene36504 | 0.814086 | 1.84E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene36504 | 0.842037 | 3.30E-58 | -1.69E-46 |
| MS.gene056609 | MS.gene36504 | 0.828044 | 1.12E-54 | -1.69E-46 |
| MS.gene056796 | MS.gene36504 | 0.81068 | 1.02E-50 | -1.69E-46 |
| MS.gene056897 | MS.gene36504 | 0.802331 | 5.90E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene36504 | 0.824858 | 6.43E-54 | -1.69E-46 |
| MS.gene057146 | MS.gene36504 | 0.81087 | 9.30E-51 | -1.69E-46 |
| MS.gene057448 | MS.gene36504 | 0.804339 | 2.27E-49 | -1.69E-46 |
| MS.gene057449 | MS.gene36504 | 0.807799 | 4.24E-50 | -1.69E-46 |
| MS.gene057450 | MS.gene36504 | 0.800721 | 1.26E-48 | -1.69E-46 |
| MS.gene057749 | MS.gene36504 | 0.817688 | 2.90E-52 | -1.69E-46 |
| MS.gene057796 | MS.gene36504 | 0.801327 | 9.49E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene36504 | 0.813489 | 2.49E-51 | -1.69E-46 |
| MS.gene058007 | MS.gene36504 | 0.807969 | 3.90E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene36504 | 0.825489 | 4.56E-54 | -1.69E-46 |
| MS.gene058376 | MS.gene36504 | 0.818536 | 1.86E-52 | -1.69E-46 |
| MS.gene05842 | MS.gene36504 | 0.807466 | 4.99E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene36504 | 0.824193 | 9.21E-54 | -1.69E-46 |
| MS.gene058928 | MS.gene36504 | 0.874547 | 6.20E-68 | -1.69E-46 |
| MS.gene05907 | MS.gene36504 | 0.836547 | 8.78E-57 | -1.69E-46 |
| MS.gene059294 | MS.gene36504 | 0.845828 | 3.18E-59 | -1.69E-46 |
| MS.gene059366 | MS.gene36504 | 0.851595 | 8.01E-61 | -1.69E-46 |
| MS.gene059420 | MS.gene36504 | 0.811993 | 5.30E-51 | -1.69E-46 |
| MS.gene059455 | MS.gene36504 | 0.803341 | 3.65E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene36504 | 0.841354 | 5.00E-58 | -1.69E-46 |
| MS.gene059597 | MS.gene36504 | 0.817561 | 3.09E-52 | -1.69E-46 |
| MS.gene059674 | MS.gene36504 | 0.809015 | 2.33E-50 | -1.69E-46 |
| MS.gene059676 | MS.gene36504 | 0.817574 | 3.07E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene36504 | 0.842429 | 2.60E-58 | -1.69E-46 |
| MS.gene059692 | MS.gene36504 | 0.830017 | 3.72E-55 | -1.69E-46 |
| MS.gene059695 | MS.gene36504 | 0.823502 | 1.34E-53 | -1.69E-46 |
| MS.gene059711 | MS.gene36504 | 0.815874 | 7.39E-52 | -1.69E-46 |
| MS.gene059807 | MS.gene36504 | 0.823513 | 1.33E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene36504 | 0.820502 | 6.63E-53 | -1.69E-46 |
| MS.gene059894 | MS.gene36504 | 0.805894 | 1.07E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene36504 | 0.809247 | 2.08E-50 | -1.69E-46 |
| MS.gene059974 | MS.gene36504 | 0.803429 | 3.50E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene36504 | 0.8255 | 4.53E-54 | -1.69E-46 |
| MS.gene060804 | MS.gene36504 | 0.829833 | 4.13E-55 | -1.69E-46 |
| MS.gene060818 | MS.gene36504 | 0.86996 | 2.09E-66 | -1.69E-46 |
| MS.gene060835 | MS.gene36504 | 0.801385 | 9.23E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene36504 | 0.863416 | 2.53E-64 | -1.69E-46 |
| MS.gene060986 | MS.gene36504 | 0.820791 | 5.69E-53 | -1.69E-46 |
| MS.gene061041 | MS.gene36504 | 0.813378 | 2.64E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene36504 | 0.80949 | 1.84E-50 | -1.69E-46 |
| MS.gene061051 | MS.gene36504 | -0.82597 | 3.50E-54 | -1.69E-46 |
| MS.gene061154 | MS.gene36504 | 0.805587 | 1.24E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36504.t1 | MTR_4g094660 | 99.091 | 330 | 2 | 1 | 1 | 330 | 459 | 787 | 0.0 | 675 |
| MS.gene36504.t1 | MTR_4g094660 | 99.091 | 330 | 2 | 1 | 1 | 330 | 273 | 601 | 0.0 | 674 |
| MS.gene36504.t1 | MTR_2g438150 | 79.701 | 335 | 59 | 5 | 1 | 330 | 459 | 789 | 0.0 | 540 |
| MS.gene36504.t1 | MTR_2g438150 | 79.104 | 335 | 61 | 5 | 1 | 330 | 459 | 789 | 0.0 | 534 |
| MS.gene36504.t1 | MTR_2g438160 | 86.335 | 161 | 22 | 0 | 1 | 161 | 459 | 619 | 4.39e-96 | 297 |
| MS.gene36504.t1 | MTR_6g033165 | 40.793 | 353 | 182 | 3 | 1 | 330 | 471 | 819 | 4.32e-86 | 276 |
| MS.gene36504.t1 | MTR_6g007697 | 40.462 | 346 | 181 | 5 | 1 | 330 | 440 | 776 | 2.13e-83 | 268 |
| MS.gene36504.t1 | MTR_5g070670 | 39.706 | 340 | 189 | 5 | 3 | 330 | 514 | 849 | 2.20e-80 | 261 |
| MS.gene36504.t1 | MTR_5g034500 | 39.017 | 346 | 190 | 5 | 1 | 330 | 442 | 782 | 7.08e-79 | 256 |
| MS.gene36504.t1 | MTR_2g008820 | 40.060 | 332 | 189 | 4 | 8 | 330 | 519 | 849 | 2.27e-78 | 256 |
| MS.gene36504.t1 | MTR_5g071827 | 39.820 | 334 | 180 | 6 | 1 | 330 | 350 | 666 | 3.22e-78 | 252 |
| MS.gene36504.t1 | MTR_5g071860 | 39.820 | 334 | 180 | 6 | 1 | 330 | 438 | 754 | 1.31e-77 | 252 |
| MS.gene36504.t1 | MTR_3g094090 | 39.222 | 334 | 174 | 4 | 26 | 330 | 276 | 609 | 1.22e-75 | 244 |
| MS.gene36504.t1 | MTR_3g094090 | 39.222 | 334 | 174 | 4 | 26 | 330 | 461 | 794 | 3.41e-74 | 244 |
| MS.gene36504.t1 | MTR_8g063840 | 39.306 | 346 | 182 | 7 | 3 | 330 | 505 | 840 | 4.73e-73 | 241 |
| MS.gene36504.t1 | MTR_8g099090 | 39.607 | 356 | 182 | 6 | 3 | 330 | 442 | 792 | 2.00e-70 | 234 |
| MS.gene36504.t1 | MTR_5g071630 | 37.425 | 334 | 179 | 7 | 1 | 330 | 418 | 725 | 4.68e-67 | 223 |
| MS.gene36504.t1 | MTR_7g108480 | 34.146 | 328 | 182 | 4 | 1 | 328 | 478 | 771 | 1.38e-64 | 218 |
| MS.gene36504.t1 | MTR_4g099260 | 36.467 | 351 | 191 | 7 | 3 | 330 | 474 | 815 | 2.13e-63 | 215 |
| MS.gene36504.t1 | MTR_8g107510 | 34.770 | 348 | 205 | 7 | 1 | 327 | 433 | 779 | 6.28e-63 | 213 |
| MS.gene36504.t1 | MTR_8g088200 | 33.718 | 347 | 203 | 6 | 3 | 330 | 444 | 782 | 1.27e-55 | 193 |
| MS.gene36504.t1 | MTR_8g063900 | 29.819 | 332 | 167 | 4 | 1 | 328 | 474 | 743 | 2.04e-54 | 189 |
| MS.gene36504.t1 | MTR_5g034590 | 47.799 | 159 | 83 | 0 | 1 | 159 | 101 | 259 | 6.77e-53 | 178 |
| MS.gene36504.t1 | MTR_8g022130 | 31.928 | 332 | 191 | 7 | 3 | 330 | 466 | 766 | 8.96e-53 | 185 |
| MS.gene36504.t1 | MTR_2g438170 | 56.790 | 81 | 26 | 5 | 192 | 267 | 1 | 77 | 1.68e-15 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36504.t1 | AT2G35060 | 71.557 | 334 | 83 | 5 | 1 | 330 | 468 | 793 | 7.16e-170 | 492 |
| MS.gene36504.t1 | AT2G35060 | 71.557 | 334 | 83 | 5 | 1 | 330 | 467 | 792 | 9.42e-170 | 491 |
| MS.gene36504.t1 | AT2G35060 | 71.557 | 334 | 83 | 5 | 1 | 330 | 467 | 792 | 9.42e-170 | 491 |
| MS.gene36504.t1 | AT2G35060 | 71.557 | 334 | 83 | 5 | 1 | 330 | 467 | 792 | 9.42e-170 | 491 |
| MS.gene36504.t1 | AT1G31120 | 72.917 | 336 | 80 | 6 | 1 | 330 | 466 | 796 | 1.07e-165 | 481 |
| MS.gene36504.t1 | AT4G19960 | 64.286 | 336 | 103 | 3 | 9 | 330 | 254 | 586 | 5.33e-140 | 409 |
| MS.gene36504.t1 | AT4G19960 | 64.286 | 336 | 103 | 3 | 9 | 330 | 475 | 807 | 6.83e-137 | 408 |
| MS.gene36504.t1 | AT4G19960 | 64.286 | 336 | 103 | 3 | 9 | 330 | 491 | 823 | 6.87e-137 | 408 |
| MS.gene36504.t1 | AT4G19960 | 64.286 | 336 | 103 | 3 | 9 | 330 | 501 | 833 | 7.08e-137 | 409 |
| MS.gene36504.t1 | AT3G02050 | 42.697 | 356 | 174 | 5 | 1 | 330 | 438 | 789 | 3.94e-86 | 275 |
| MS.gene36504.t1 | AT4G33530 | 41.420 | 338 | 187 | 4 | 3 | 330 | 291 | 627 | 1.31e-84 | 268 |
| MS.gene36504.t1 | AT4G33530 | 41.420 | 338 | 187 | 4 | 3 | 330 | 519 | 855 | 6.20e-83 | 268 |
| MS.gene36504.t1 | AT5G09400 | 41.228 | 342 | 186 | 5 | 3 | 330 | 518 | 858 | 3.75e-80 | 261 |
| MS.gene36504.t1 | AT2G40540 | 39.607 | 356 | 187 | 5 | 3 | 330 | 439 | 794 | 8.92e-80 | 259 |
| MS.gene36504.t1 | AT2G40540 | 39.607 | 356 | 187 | 5 | 3 | 330 | 439 | 794 | 8.92e-80 | 259 |
| MS.gene36504.t1 | AT2G40540 | 39.607 | 356 | 187 | 5 | 3 | 330 | 439 | 794 | 8.92e-80 | 259 |
| MS.gene36504.t1 | AT2G40540 | 39.607 | 356 | 187 | 5 | 3 | 330 | 439 | 794 | 8.92e-80 | 259 |
| MS.gene36504.t1 | AT2G40540 | 39.607 | 356 | 187 | 5 | 3 | 330 | 439 | 794 | 8.92e-80 | 259 |
| MS.gene36504.t1 | AT1G60160 | 38.024 | 334 | 193 | 4 | 3 | 330 | 502 | 827 | 7.64e-78 | 254 |
| MS.gene36504.t1 | AT5G14880 | 39.942 | 343 | 191 | 4 | 1 | 330 | 441 | 781 | 2.16e-74 | 244 |
| MS.gene36504.t1 | AT4G23640 | 38.418 | 354 | 192 | 5 | 1 | 330 | 424 | 775 | 2.48e-74 | 244 |
| MS.gene36504.t1 | AT1G70300 | 36.152 | 343 | 205 | 3 | 1 | 330 | 441 | 782 | 7.27e-69 | 229 |
| MS.gene36504.t1 | AT4G13420 | 34.049 | 326 | 206 | 1 | 3 | 328 | 467 | 783 | 1.78e-63 | 215 |
| MS.gene36504.t1 | AT2G30070 | 50.000 | 186 | 93 | 0 | 1 | 186 | 318 | 503 | 3.92e-61 | 205 |
| MS.gene36504.t1 | AT2G30070 | 38.961 | 77 | 47 | 0 | 254 | 330 | 516 | 592 | 1.39e-12 | 68.9 |
| MS.gene36504.t1 | AT2G30070 | 50.000 | 186 | 93 | 0 | 1 | 186 | 438 | 623 | 2.13e-60 | 205 |
| MS.gene36504.t1 | AT2G30070 | 38.961 | 77 | 47 | 0 | 254 | 330 | 636 | 712 | 1.72e-12 | 68.6 |
Find 77 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAGGTATCTCTCTTCTTC+CGG | 0.262164 | 4.1:-18135076 | None:intergenic |
| GCTGGGGTGGTTCACATATT+AGG | 0.264983 | 4.1:+18135475 | MS.gene36504:CDS |
| ATTATATATATGCATTTCTT+AGG | 0.280181 | 4.1:+18135554 | MS.gene36504:CDS |
| TCAGATGAATACAGTTTATA+TGG | 0.316978 | 4.1:+18135256 | MS.gene36504:CDS |
| CTCGGGCTTGGCCCAAGTTT+AGG | 0.337026 | 4.1:+18134905 | MS.gene36504:CDS |
| CAAGGTATCTCTCTTCTTCC+GGG | 0.346404 | 4.1:-18135075 | None:intergenic |
| CGAAAGATGTGGTAATTCTT+AGG | 0.358981 | 4.1:-18135112 | None:intergenic |
| ATTATGTTTGTCTGGCATTA+CGG | 0.372759 | 4.1:+18134827 | MS.gene36504:CDS |
| GTCTCGATGGCATGGATTCT+CGG | 0.378294 | 4.1:+18134887 | MS.gene36504:CDS |
| TGGGTTCCTCTTGTAATTGC+CGG | 0.384804 | 4.1:+18134791 | MS.gene36504:CDS |
| ATGGCATGGATTCTCGGGCT+TGG | 0.385857 | 4.1:+18134893 | MS.gene36504:CDS |
| TTAGGACTTGTTCGTGTACC+TGG | 0.405042 | 4.1:+18134923 | MS.gene36504:CDS |
| GTGTAGACTAGTCCGATTCC+AGG | 0.417435 | 4.1:-18134941 | None:intergenic |
| TCTTCCGGGACGGTGTAAAC+AGG | 0.434145 | 4.1:-18135061 | None:intergenic |
| ACTCTTCGTCTTTCTTGTGC+AGG | 0.434280 | 4.1:-18135162 | None:intergenic |
| TTGAATAACTGCAGGGACGC+TGG | 0.441557 | 4.1:+18135457 | MS.gene36504:CDS |
| TCTCGATGGCATGGATTCTC+GGG | 0.451131 | 4.1:+18134888 | MS.gene36504:CDS |
| CTAACAGTGATATTCACATT+TGG | 0.460145 | 4.1:-18135385 | None:intergenic |
| CAAATGTGAATATCACTGTT+AGG | 0.464701 | 4.1:+18135386 | MS.gene36504:CDS |
| TATTGGTGTGGCGCTGCCAC+TGG | 0.464822 | 4.1:+18134681 | MS.gene36504:CDS |
| TGAATAACTGCAGGGACGCT+GGG | 0.476846 | 4.1:+18135458 | MS.gene36504:CDS |
| TCAAAGTTAATCAAGGTGGA+TGG | 0.480215 | 4.1:+18134771 | MS.gene36504:CDS |
| CTTGTTCGTGTACCTGGAAT+CGG | 0.484864 | 4.1:+18134929 | MS.gene36504:CDS |
| AGATACCTTGTAAAGAGGAT+TGG | 0.486463 | 4.1:+18135088 | MS.gene36504:CDS |
| TTTCGATGTGTGGCACGGTA+TGG | 0.488737 | 4.1:+18135130 | MS.gene36504:CDS |
| CATTTCTTAGGAAAATATGC+AGG | 0.492742 | 4.1:+18135566 | MS.gene36504:CDS |
| AAGAGAGATACCTTGTAAAG+AGG | 0.504738 | 4.1:+18135083 | MS.gene36504:CDS |
| TAGGTCCAATCCTCTTTACA+AGG | 0.508685 | 4.1:-18135093 | None:intergenic |
| GTAATCTTCAAAGTTAATCA+AGG | 0.509932 | 4.1:+18134764 | MS.gene36504:CDS |
| CACTTGCTTATCATTGATTG+TGG | 0.513008 | 4.1:+18134721 | MS.gene36504:CDS |
| CAAGCTTAACAAACAGTGAA+AGG | 0.516927 | 4.1:-18135207 | None:intergenic |
| ACAGTGATATTCACATTTGG+CGG | 0.519018 | 4.1:-18135382 | None:intergenic |
| ACACGAACAAGTCCTAAACT+TGG | 0.520151 | 4.1:-18134917 | None:intergenic |
| CACAGTTGTGATGGCAAGGA+GGG | 0.523327 | 4.1:+18135501 | MS.gene36504:CDS |
| CAGTTTATATGGAGGACAGC+AGG | 0.530247 | 4.1:+18135267 | MS.gene36504:CDS |
| CAAAGTTAATCAAGGTGGAT+GGG | 0.539107 | 4.1:+18134772 | MS.gene36504:CDS |
| TGTTAAGCTTGAATCTATGA+TGG | 0.539727 | 4.1:+18135219 | MS.gene36504:CDS |
| CATGAGAGTCTCTTAAATGT+TGG | 0.554708 | 4.1:+18135616 | MS.gene36504:CDS |
| ACAACAACAGAATGGATGGC+TGG | 0.560974 | 4.1:-18135016 | None:intergenic |
| AGGTCCTGCGGTCAAGTAAG+TGG | 0.564330 | 4.1:+18135406 | MS.gene36504:CDS |
| CCTTCCTGTTTACACCGTCC+CGG | 0.569084 | 4.1:+18135057 | MS.gene36504:CDS |
| TTGGCCACTTACTTGACCGC+AGG | 0.570429 | 4.1:-18135410 | None:intergenic |
| CAAGTAAGTGGCCAAACCGA+GGG | 0.574154 | 4.1:+18135418 | MS.gene36504:CDS |
| CAACAGAATGGATGGCTGGT+AGG | 0.574816 | 4.1:-18135012 | None:intergenic |
| TAACTGCAGGGACGCTGGGG+TGG | 0.576829 | 4.1:+18135462 | MS.gene36504:CDS |
| GTATCTCTCTTCTTCCGGGA+CGG | 0.583221 | 4.1:-18135071 | None:intergenic |
| GTCTACACAGAACTTGCAAG+TGG | 0.583444 | 4.1:+18134956 | MS.gene36504:CDS |
| AGAATGGATGGCTGGTAGGT+TGG | 0.583797 | 4.1:-18135008 | None:intergenic |
| ATTAGGAAACACAGTTGTGA+TGG | 0.587624 | 4.1:+18135492 | MS.gene36504:CDS |
| GAAACACAGTTGTGATGGCA+AGG | 0.591010 | 4.1:+18135497 | MS.gene36504:CDS |
| GTGATATTCACATTTGGCGG+CGG | 0.592182 | 4.1:-18135379 | None:intergenic |
| TCAAGTAAGTGGCCAAACCG+AGG | 0.600769 | 4.1:+18135417 | MS.gene36504:CDS |
| TTACCACATCTTTCGATGTG+TGG | 0.605821 | 4.1:+18135120 | MS.gene36504:CDS |
| CACGAACAAGTCCTAAACTT+GGG | 0.614583 | 4.1:-18134916 | None:intergenic |
| ATAGTAAAGTCTCGATGGCA+TGG | 0.620677 | 4.1:+18134879 | MS.gene36504:CDS |
| AATATCACTGTTAGGTCCTG+CGG | 0.624494 | 4.1:+18135394 | MS.gene36504:CDS |
| AAGCACGACTTCTTCGAATG+TGG | 0.624780 | 4.1:+18135312 | MS.gene36504:CDS |
| AATCAATGATAAGCAAGTGA+AGG | 0.625371 | 4.1:-18134717 | None:intergenic |
| ATTTCTTAGGAAAATATGCA+GGG | 0.625694 | 4.1:+18135567 | MS.gene36504:CDS |
| AACACTCCGGCAATTACAAG+AGG | 0.633389 | 4.1:-18134797 | None:intergenic |
| ATCTTCAAAGTTAATCAAGG+TGG | 0.637689 | 4.1:+18134767 | MS.gene36504:CDS |
| AATGCATAGTAAAGTCTCGA+TGG | 0.638063 | 4.1:+18134874 | MS.gene36504:CDS |
| AAGCTTGAATCTATGATGGA+AGG | 0.641141 | 4.1:+18135223 | MS.gene36504:CDS |
| GAATAACTGCAGGGACGCTG+GGG | 0.645282 | 4.1:+18135459 | MS.gene36504:CDS |
| ACATCTTTCGATGTGTGGCA+CGG | 0.647935 | 4.1:+18135125 | MS.gene36504:CDS |
| GATGAATACAGTTTATATGG+AGG | 0.656772 | 4.1:+18135259 | MS.gene36504:CDS |
| CCGGGACGGTGTAAACAGGA+AGG | 0.657637 | 4.1:-18135057 | None:intergenic |
| TTATATGGAGGACAGCAGGA+TGG | 0.666470 | 4.1:+18135271 | MS.gene36504:CDS |
| ACACAGTTGTGATGGCAAGG+AGG | 0.669872 | 4.1:+18135500 | MS.gene36504:CDS |
| GTGCCACACATCGAAAGATG+TGG | 0.686236 | 4.1:-18135123 | None:intergenic |
| GTGATGAAATGAGAAAAGAT+CGG | 0.689117 | 4.1:-18134986 | None:intergenic |
| AAGTAAGTGGCCAAACCGAG+GGG | 0.689786 | 4.1:+18135419 | MS.gene36504:CDS |
| ATGAAATGAGAAAAGATCGG+TGG | 0.702922 | 4.1:-18134983 | None:intergenic |
| AGTAAGTGGCCAAACCGAGG+GGG | 0.709182 | 4.1:+18135420 | MS.gene36504:CDS |
| ACATTTAAGAGACTCTCATG+AGG | 0.718347 | 4.1:-18135613 | None:intergenic |
| GATGGCGTGTTGAATAACAA+CGG | 0.731429 | 4.1:+18135289 | MS.gene36504:CDS |
| AGGCGAGTACAAGAATCCAG+TGG | 0.751362 | 4.1:-18134697 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATATATATGCATTTCTT+AGG | + | chr4.1:18135554-18135573 | MS.gene36504:CDS | 15.0% |
| !!! | TTTTTATCATTATGTTTGTC+TGG | + | chr4.1:18134819-18134838 | MS.gene36504:CDS | 20.0% |
| ! | ATACAAAAACAACAACAGAA+TGG | - | chr4.1:18135027-18135046 | None:intergenic | 25.0% |
| ! | ATTTCTTAGGAAAATATGCA+GGG | + | chr4.1:18135567-18135586 | MS.gene36504:CDS | 25.0% |
| ! | CATAATGATAAAAAACACTC+CGG | - | chr4.1:18134813-18134832 | None:intergenic | 25.0% |
| ! | GTAATCTTCAAAGTTAATCA+AGG | + | chr4.1:18134764-18134783 | MS.gene36504:CDS | 25.0% |
| ! | TCAGATGAATACAGTTTATA+TGG | + | chr4.1:18135256-18135275 | MS.gene36504:CDS | 25.0% |
| !! | TGAATTTTTGAATAACTGCA+GGG | + | chr4.1:18135450-18135469 | MS.gene36504:CDS | 25.0% |
| !! | TTGAATTTTTGAATAACTGC+AGG | + | chr4.1:18135449-18135468 | MS.gene36504:CDS | 25.0% |
| !! | TTTTAATCATGATATTGGTG+TGG | + | chr4.1:18134669-18134688 | MS.gene36504:CDS | 25.0% |
| AAAAACAACAACAGAATGGA+TGG | - | chr4.1:18135023-18135042 | None:intergenic | 30.0% | |
| AATCAATGATAAGCAAGTGA+AGG | - | chr4.1:18134720-18134739 | None:intergenic | 30.0% | |
| ATCTTCAAAGTTAATCAAGG+TGG | + | chr4.1:18134767-18134786 | MS.gene36504:CDS | 30.0% | |
| ATTATGTTTGTCTGGCATTA+CGG | + | chr4.1:18134827-18134846 | MS.gene36504:CDS | 30.0% | |
| CAAATGTGAATATCACTGTT+AGG | + | chr4.1:18135386-18135405 | MS.gene36504:CDS | 30.0% | |
| CATTTCTTAGGAAAATATGC+AGG | + | chr4.1:18135566-18135585 | MS.gene36504:CDS | 30.0% | |
| CTAACAGTGATATTCACATT+TGG | - | chr4.1:18135388-18135407 | None:intergenic | 30.0% | |
| GATGAATACAGTTTATATGG+AGG | + | chr4.1:18135259-18135278 | MS.gene36504:CDS | 30.0% | |
| GTGATGAAATGAGAAAAGAT+CGG | - | chr4.1:18134989-18135008 | None:intergenic | 30.0% | |
| TGTTAAGCTTGAATCTATGA+TGG | + | chr4.1:18135219-18135238 | MS.gene36504:CDS | 30.0% | |
| AAGAGAGATACCTTGTAAAG+AGG | + | chr4.1:18135083-18135102 | MS.gene36504:CDS | 35.0% | |
| AATGCATAGTAAAGTCTCGA+TGG | + | chr4.1:18134874-18134893 | MS.gene36504:CDS | 35.0% | |
| ACAGTGATATTCACATTTGG+CGG | - | chr4.1:18135385-18135404 | None:intergenic | 35.0% | |
| ACATTTAAGAGACTCTCATG+AGG | - | chr4.1:18135616-18135635 | None:intergenic | 35.0% | |
| AGATACCTTGTAAAGAGGAT+TGG | + | chr4.1:18135088-18135107 | MS.gene36504:CDS | 35.0% | |
| ATGAAATGAGAAAAGATCGG+TGG | - | chr4.1:18134986-18135005 | None:intergenic | 35.0% | |
| ATTAGGAAACACAGTTGTGA+TGG | + | chr4.1:18135492-18135511 | MS.gene36504:CDS | 35.0% | |
| CAAAGTTAATCAAGGTGGAT+GGG | + | chr4.1:18134772-18134791 | MS.gene36504:CDS | 35.0% | |
| CAAGCTTAACAAACAGTGAA+AGG | - | chr4.1:18135210-18135229 | None:intergenic | 35.0% | |
| CATGAGAGTCTCTTAAATGT+TGG | + | chr4.1:18135616-18135635 | MS.gene36504:CDS | 35.0% | |
| CGAAAGATGTGGTAATTCTT+AGG | - | chr4.1:18135115-18135134 | None:intergenic | 35.0% | |
| TCAAAGTTAATCAAGGTGGA+TGG | + | chr4.1:18134771-18134790 | MS.gene36504:CDS | 35.0% | |
| ! | AAGCTTGAATCTATGATGGA+AGG | + | chr4.1:18135223-18135242 | MS.gene36504:CDS | 35.0% |
| ! | CACTTGCTTATCATTGATTG+TGG | + | chr4.1:18134721-18134740 | MS.gene36504:CDS | 35.0% |
| AATATCACTGTTAGGTCCTG+CGG | + | chr4.1:18135394-18135413 | MS.gene36504:CDS | 40.0% | |
| ACACGAACAAGTCCTAAACT+TGG | - | chr4.1:18134920-18134939 | None:intergenic | 40.0% | |
| ATAGTAAAGTCTCGATGGCA+TGG | + | chr4.1:18134879-18134898 | MS.gene36504:CDS | 40.0% | |
| CACGAACAAGTCCTAAACTT+GGG | - | chr4.1:18134919-18134938 | None:intergenic | 40.0% | |
| GATGGCGTGTTGAATAACAA+CGG | + | chr4.1:18135289-18135308 | MS.gene36504:CDS | 40.0% | |
| TAGGTCCAATCCTCTTTACA+AGG | - | chr4.1:18135096-18135115 | None:intergenic | 40.0% | |
| TTACCACATCTTTCGATGTG+TGG | + | chr4.1:18135120-18135139 | MS.gene36504:CDS | 40.0% | |
| ! | AAATTCAAGTTCATCCCCCT+CGG | - | chr4.1:18135437-18135456 | None:intergenic | 40.0% |
| ! | ACAAGGTATCTCTCTTCTTC+CGG | - | chr4.1:18135079-18135098 | None:intergenic | 40.0% |
| AACACTCCGGCAATTACAAG+AGG | - | chr4.1:18134800-18134819 | None:intergenic | 45.0% | |
| AAGCACGACTTCTTCGAATG+TGG | + | chr4.1:18135312-18135331 | MS.gene36504:CDS | 45.0% | |
| ACAACAACAGAATGGATGGC+TGG | - | chr4.1:18135019-18135038 | None:intergenic | 45.0% | |
| ACATCTTTCGATGTGTGGCA+CGG | + | chr4.1:18135125-18135144 | MS.gene36504:CDS | 45.0% | |
| ACTCTTCGTCTTTCTTGTGC+AGG | - | chr4.1:18135165-18135184 | None:intergenic | 45.0% | |
| CAGTTTATATGGAGGACAGC+AGG | + | chr4.1:18135267-18135286 | MS.gene36504:CDS | 45.0% | |
| CTTGTTCGTGTACCTGGAAT+CGG | + | chr4.1:18134929-18134948 | MS.gene36504:CDS | 45.0% | |
| GAAACACAGTTGTGATGGCA+AGG | + | chr4.1:18135497-18135516 | MS.gene36504:CDS | 45.0% | |
| GTCTACACAGAACTTGCAAG+TGG | + | chr4.1:18134956-18134975 | MS.gene36504:CDS | 45.0% | |
| GTGATATTCACATTTGGCGG+CGG | - | chr4.1:18135382-18135401 | None:intergenic | 45.0% | |
| TGGGTTCCTCTTGTAATTGC+CGG | + | chr4.1:18134791-18134810 | MS.gene36504:CDS | 45.0% | |
| TTATATGGAGGACAGCAGGA+TGG | + | chr4.1:18135271-18135290 | MS.gene36504:CDS | 45.0% | |
| ! | CAAGGTATCTCTCTTCTTCC+GGG | - | chr4.1:18135078-18135097 | None:intergenic | 45.0% |
| ! | TTAGGACTTGTTCGTGTACC+TGG | + | chr4.1:18134923-18134942 | MS.gene36504:CDS | 45.0% |
| AAGTAAGTGGCCAAACCGAG+GGG | + | chr4.1:18135419-18135438 | MS.gene36504:CDS | 50.0% | |
| ACACAGTTGTGATGGCAAGG+AGG | + | chr4.1:18135500-18135519 | MS.gene36504:CDS | 50.0% | |
| AGAATGGATGGCTGGTAGGT+TGG | - | chr4.1:18135011-18135030 | None:intergenic | 50.0% | |
| AGGCGAGTACAAGAATCCAG+TGG | - | chr4.1:18134700-18134719 | None:intergenic | 50.0% | |
| CAACAGAATGGATGGCTGGT+AGG | - | chr4.1:18135015-18135034 | None:intergenic | 50.0% | |
| CAAGTAAGTGGCCAAACCGA+GGG | + | chr4.1:18135418-18135437 | MS.gene36504:CDS | 50.0% | |
| CACAGTTGTGATGGCAAGGA+GGG | + | chr4.1:18135501-18135520 | MS.gene36504:CDS | 50.0% | |
| GCTGGGGTGGTTCACATATT+AGG | + | chr4.1:18135475-18135494 | MS.gene36504:CDS | 50.0% | |
| GTATCTCTCTTCTTCCGGGA+CGG | - | chr4.1:18135074-18135093 | None:intergenic | 50.0% | |
| GTCTCGATGGCATGGATTCT+CGG | + | chr4.1:18134887-18134906 | MS.gene36504:CDS | 50.0% | |
| GTGCCACACATCGAAAGATG+TGG | - | chr4.1:18135126-18135145 | None:intergenic | 50.0% | |
| TCAAGTAAGTGGCCAAACCG+AGG | + | chr4.1:18135417-18135436 | MS.gene36504:CDS | 50.0% | |
| TCTCGATGGCATGGATTCTC+GGG | + | chr4.1:18134888-18134907 | MS.gene36504:CDS | 50.0% | |
| TGAATAACTGCAGGGACGCT+GGG | + | chr4.1:18135458-18135477 | MS.gene36504:CDS | 50.0% | |
| TTGAATAACTGCAGGGACGC+TGG | + | chr4.1:18135457-18135476 | MS.gene36504:CDS | 50.0% | |
| TTTCGATGTGTGGCACGGTA+TGG | + | chr4.1:18135130-18135149 | MS.gene36504:CDS | 50.0% | |
| ! | GTGTAGACTAGTCCGATTCC+AGG | - | chr4.1:18134944-18134963 | None:intergenic | 50.0% |
| AGGTCCTGCGGTCAAGTAAG+TGG | + | chr4.1:18135406-18135425 | MS.gene36504:CDS | 55.0% | |
| AGTAAGTGGCCAAACCGAGG+GGG | + | chr4.1:18135420-18135439 | MS.gene36504:CDS | 55.0% | |
| ATGGCATGGATTCTCGGGCT+TGG | + | chr4.1:18134893-18134912 | MS.gene36504:CDS | 55.0% | |
| CAAGTTCATCCCCCTCGGTT+TGG | - | chr4.1:18135432-18135451 | None:intergenic | 55.0% | |
| CCTTCCTGTTTACACCGTCC+CGG | + | chr4.1:18135057-18135076 | MS.gene36504:CDS | 55.0% | |
| GAATAACTGCAGGGACGCTG+GGG | + | chr4.1:18135459-18135478 | MS.gene36504:CDS | 55.0% | |
| TCTTCCGGGACGGTGTAAAC+AGG | - | chr4.1:18135064-18135083 | None:intergenic | 55.0% | |
| TTGGCCACTTACTTGACCGC+AGG | - | chr4.1:18135413-18135432 | None:intergenic | 55.0% | |
| CCGGGACGGTGTAAACAGGA+AGG | - | chr4.1:18135060-18135079 | None:intergenic | 60.0% | |
| CTCGGGCTTGGCCCAAGTTT+AGG | + | chr4.1:18134905-18134924 | MS.gene36504:CDS | 60.0% | |
| TATTGGTGTGGCGCTGCCAC+TGG | + | chr4.1:18134681-18134700 | MS.gene36504:CDS | 60.0% | |
| TAACTGCAGGGACGCTGGGG+TGG | + | chr4.1:18135462-18135481 | MS.gene36504:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 18134665 | 18135657 | 18134665 | ID=MS.gene36504 |
| chr4.1 | mRNA | 18134665 | 18135657 | 18134665 | ID=MS.gene36504.t1;Parent=MS.gene36504 |
| chr4.1 | exon | 18134665 | 18135657 | 18134665 | ID=MS.gene36504.t1.exon1;Parent=MS.gene36504.t1 |
| chr4.1 | CDS | 18134665 | 18135657 | 18134665 | ID=cds.MS.gene36504.t1;Parent=MS.gene36504.t1 |
| Gene Sequence |
| Protein sequence |
