AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057244


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057244.t1 MTR_8g107310 38.028 142 62 4 80 196 152 292 7.36e-20 85.1
MS.gene057244.t1 MTR_5g017130 32.558 215 87 9 1 196 1 176 6.53e-19 80.5
MS.gene057244.t1 MTR_8g031560 41.322 121 57 4 88 196 162 280 2.46e-17 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057244.t1 AT5G06000 45.794 107 44 3 102 195 181 286 5.06e-18 80.5
MS.gene057244.t1 AT3G11400 45.370 108 45 4 102 196 188 294 5.81e-18 80.1
MS.gene057244.t1 AT3G11400 45.370 108 45 4 102 196 215 321 1.06e-17 79.7

Find 52 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGCAATCGAGAAGTTTA+AGG 0.247977 3.2:-77991304 MS.gene057244:CDS
TCTGGCCTTACTTGGTTCTT+TGG 0.248015 3.2:-77990474 MS.gene057244:CDS
GCTGAACTCTGCCGTCCTTA+TGG 0.275421 3.2:-77990534 MS.gene057244:CDS
TTGGTAGGAGGAGGAGATTT+GGG 0.343798 3.2:+77991261 None:intergenic
TGACAACGCTAATTCTGTTC+TGG 0.345295 3.2:-77990598 MS.gene057244:CDS
TTTGGTAGGAGGAGGAGATT+TGG 0.345311 3.2:+77991260 None:intergenic
ATCAAGAGTCTGGCCTTACT+TGG 0.397071 3.2:-77990482 MS.gene057244:CDS
CGTCGTAAGGAACTCTGATT+TGG 0.401539 3.2:-77990556 MS.gene057244:CDS
AGGAGGAGATTTGGGTTCAG+AGG 0.452700 3.2:+77991269 None:intergenic
AGAATTAGCGTTGTCAAGAT+AGG 0.456091 3.2:+77990605 None:intergenic
TATGTACCTACAGTCAAGAT+TGG 0.456722 3.2:-77990681 MS.gene057244:CDS
GCTTTGTTAGCTTTGCCACC+AGG 0.462501 3.2:-77990452 MS.gene057244:CDS
AATGCAATCGAGAAGTTTAA+GGG 0.473415 3.2:-77991303 MS.gene057244:CDS
CGAGGACAATGTATTGCAAC+CGG 0.485814 3.2:+77990729 None:intergenic
GTTGCTATTGATCAAGAGTC+TGG 0.486644 3.2:-77990492 MS.gene057244:CDS
CTTGACTGTAGGTACATACT+CGG 0.492126 3.2:+77990686 None:intergenic
ACCTCTCCTTCGACGTCGTA+AGG 0.500333 3.2:-77990569 MS.gene057244:CDS
ACAAAAGCTTCCATAAACTT+AGG 0.510191 3.2:+77990645 None:intergenic
CAAAGCCAAAGAACCAAGTA+AGG 0.522006 3.2:+77990469 None:intergenic
ATCAATAGCAACATCGGCAC+TGG 0.526411 3.2:+77990503 None:intergenic
ATGTACCTACAGTCAAGATT+GGG 0.531893 3.2:-77990680 MS.gene057244:CDS
GAGACGGTCGTGATCTCGAC+CGG 0.543343 3.2:-77991335 MS.gene057244:CDS
GAGCGTAGTTTGGTAGGAGG+AGG 0.548480 3.2:+77991252 None:intergenic
TTGACTGTAGGTACATACTC+GGG 0.548633 3.2:+77990687 None:intergenic
TTCACCGTCGCTGTCGCTGT+CGG 0.556222 3.2:+77991176 None:intergenic
ATTCGTTCATGCCTCTTAGG+AGG 0.559458 3.2:+77990368 None:intergenic
GCACTGGTCACATTTCCATA+AGG 0.565262 3.2:+77990519 None:intergenic
TCCTTACGACGTCGAAGGAG+AGG 0.565857 3.2:+77990568 None:intergenic
CTTTGTTAGCTTTGCCACCA+GGG 0.571161 3.2:-77990451 MS.gene057244:CDS
ACAATCTCGATGGAGTTCAA+TGG 0.571716 3.2:-77990407 MS.gene057244:CDS
AACAATGAGCGTAGTTTGGT+AGG 0.576703 3.2:+77991246 None:intergenic
AGCATTCTGAGCATCGTCCC+TGG 0.580578 3.2:+77990434 None:intergenic
GTGAACTCACGGTGTGGAGA+CGG 0.584086 3.2:-77991351 MS.gene057244:CDS
AGAGTTCCTTACGACGTCGA+AGG 0.585755 3.2:+77990563 None:intergenic
GGCTGCGATTGAGAGAGAAC+TGG 0.594127 3.2:-77991373 MS.gene057244:CDS
AATGCTATTAACAATCTCGA+TGG 0.598791 3.2:-77990417 MS.gene057244:CDS
TGACTGTAGGTACATACTCG+GGG 0.600362 3.2:+77990688 None:intergenic
CTCTTGATCAATAGCAACAT+CGG 0.606132 3.2:+77990497 None:intergenic
AGAGAGAACTGGTGAACTCA+CGG 0.607110 3.2:-77991362 MS.gene057244:CDS
AAACTCCCAATCTTGACTGT+AGG 0.615432 3.2:+77990675 None:intergenic
TACGACGTCGAAGGAGAGGT+CGG 0.621329 3.2:+77990572 None:intergenic
TGGTCACATTTCCATAAGGA+CGG 0.636508 3.2:+77990523 None:intergenic
AATGAGCGTAGTTTGGTAGG+AGG 0.640190 3.2:+77991249 None:intergenic
GACTGTAGGTACATACTCGG+GGG 0.640689 3.2:+77990689 None:intergenic
TGTCGGTGTCGATGCTGTCG+AGG 0.641023 3.2:+77991193 None:intergenic
CGATGCTGTCGAGGTCGCTG+TGG 0.657765 3.2:+77991202 None:intergenic
GACACCGACAGCGACAGCGA+CGG 0.673613 3.2:-77991180 MS.gene057244:CDS
GAACTGGTGAACTCACGGTG+TGG 0.694794 3.2:-77991357 MS.gene057244:CDS
ATTCTGAGCATCGTCCCTGG+TGG 0.711419 3.2:+77990437 None:intergenic
GCAATCGAGAAGTTTAAGGG+CGG 0.727442 3.2:-77991300 MS.gene057244:CDS
ACTGTAGGTACATACTCGGG+GGG 0.731660 3.2:+77990690 None:intergenic
GGTTGTTGACAAGTACATCG+AGG 0.785440 3.2:+77990711 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAAAATTGAATGTAAT+AGG + chr3.2:77990635-77990654 None:intergenic 10.0%
!! AATTCAATTCATTATATTGT+TGG + chr3.2:77990802-77990821 None:intergenic 15.0%
!! ATTGAATGTAATAGGAAAAA+AGG + chr3.2:77990627-77990646 None:intergenic 20.0%
!! TTGAATGTAATAGGAAAAAA+GGG + chr3.2:77990626-77990645 None:intergenic 20.0%
!!! AACAATATTGAAATTTGCTT+AGG + chr3.2:77990672-77990691 None:intergenic 20.0%
! AATTCATTATATTGTTGGAC+TGG + chr3.2:77990797-77990816 None:intergenic 25.0%
! TGAATGTAATAGGAAAAAAG+GGG + chr3.2:77990625-77990644 None:intergenic 25.0%
! TTAATCTCATTTGTATCTAC+AGG - chr3.2:77990916-77990935 MS.gene057244:intron 25.0%
AAAAAACAATGAGCGTAGTT+TGG + chr3.2:77990519-77990538 None:intergenic 30.0%
AAATGCAATCGAGAAGTTTA+AGG - chr3.2:77990454-77990473 MS.gene057244:CDS 30.0%
AATAGTGATATTAACCCACT+AGG + chr3.2:77990954-77990973 None:intergenic 30.0%
AATGCAATCGAGAAGTTTAA+GGG - chr3.2:77990455-77990474 MS.gene057244:CDS 30.0%
AATGCTATTAACAATCTCGA+TGG - chr3.2:77991341-77991360 MS.gene057244:CDS 30.0%
ACAAAAGCTTCCATAAACTT+AGG + chr3.2:77991116-77991135 None:intergenic 30.0%
ATAGTGATATTAACCCACTA+GGG + chr3.2:77990953-77990972 None:intergenic 30.0%
TCATTTGTATCTACAGGTTT+CGG - chr3.2:77990922-77990941 MS.gene057244:intron 30.0%
TGTTGAAAACTCGTTAAAGA+TGG - chr3.2:77990700-77990719 MS.gene057244:CDS 30.0%
! TAAGTTTATGGAAGCTTTTG+TGG - chr3.2:77991115-77991134 MS.gene057244:intron 30.0%
! TTTTCACGTACCTAAGTTTA+TGG - chr3.2:77991103-77991122 MS.gene057244:intron 30.0%
AGAATTAGCGTTGTCAAGAT+AGG + chr3.2:77991156-77991175 None:intergenic 35.0%
ATGTACCTACAGTCAAGATT+GGG - chr3.2:77991078-77991097 MS.gene057244:intron 35.0%
CATTGTAGTCTGTGATACAT+CGG + chr3.2:77990869-77990888 None:intergenic 35.0%
CTCTTGATCAATAGCAACAT+CGG + chr3.2:77991264-77991283 None:intergenic 35.0%
TATGTACCTACAGTCAAGAT+TGG - chr3.2:77991077-77991096 MS.gene057244:intron 35.0%
TTAGTAAAAGCCAACTAGGA+TGG + chr3.2:77990774-77990793 None:intergenic 35.0%
! GCTTTTGTGGATAAGATGAT+TGG - chr3.2:77991128-77991147 MS.gene057244:intron 35.0%
!!! GCTCATTGTTTTTTGACGAA+TGG - chr3.2:77990525-77990544 MS.gene057244:CDS 35.0%
AAACTCCCAATCTTGACTGT+AGG + chr3.2:77991086-77991105 None:intergenic 40.0%
AACAATGAGCGTAGTTTGGT+AGG + chr3.2:77990515-77990534 None:intergenic 40.0%
ACAATCTCGATGGAGTTCAA+TGG - chr3.2:77991351-77991370 MS.gene057244:CDS 40.0%
CAAAGCCAAAGAACCAAGTA+AGG + chr3.2:77991292-77991311 None:intergenic 40.0%
CTGGTTAGTAAAAGCCAACT+AGG + chr3.2:77990778-77990797 None:intergenic 40.0%
CTTGACTGTAGGTACATACT+CGG + chr3.2:77991075-77991094 None:intergenic 40.0%
TAGTCTGTGATACATCGGAA+CGG + chr3.2:77990864-77990883 None:intergenic 40.0%
TGACAACGCTAATTCTGTTC+TGG - chr3.2:77991160-77991179 MS.gene057244:intron 40.0%
TGATATTAACCCACTAGGGT+TGG + chr3.2:77990949-77990968 None:intergenic 40.0%
TGGTCACATTTCCATAAGGA+CGG + chr3.2:77991238-77991257 None:intergenic 40.0%
TTGACTGTAGGTACATACTC+GGG + chr3.2:77991074-77991093 None:intergenic 40.0%
! GTTGCTATTGATCAAGAGTC+TGG - chr3.2:77991266-77991285 MS.gene057244:CDS 40.0%
! TTCTCGATTGCATTTTCGTC+AGG + chr3.2:77990449-77990468 None:intergenic 40.0%
AAAGCCAACTAGGATGGTGT+TGG + chr3.2:77990768-77990787 None:intergenic 45.0%
AATGAGCGTAGTTTGGTAGG+AGG + chr3.2:77990512-77990531 None:intergenic 45.0%
AGAGAGAACTGGTGAACTCA+CGG - chr3.2:77990396-77990415 MS.gene057244:CDS 45.0%
ATCAATAGCAACATCGGCAC+TGG + chr3.2:77991258-77991277 None:intergenic 45.0%
ATCCCACCGAAATTGACATC+AGG - chr3.2:77990728-77990747 MS.gene057244:CDS 45.0%
CCTGTTTGTATCTACAGGTC+CGG - chr3.2:77991010-77991029 MS.gene057244:intron 45.0%
CGAGGACAATGTATTGCAAC+CGG + chr3.2:77991032-77991051 None:intergenic 45.0%
CGGACCTGTAGATACAAACA+GGG + chr3.2:77991012-77991031 None:intergenic 45.0%
CGTCGTAAGGAACTCTGATT+TGG - chr3.2:77991202-77991221 MS.gene057244:CDS 45.0%
CTGTAGATACAAACAGGGAC+AGG + chr3.2:77991007-77991026 None:intergenic 45.0%
CTGTCCCTGTTTGTATCTAC+AGG - chr3.2:77991005-77991024 MS.gene057244:intron 45.0%
GCAATCGAGAAGTTTAAGGG+CGG - chr3.2:77990458-77990477 MS.gene057244:CDS 45.0%
GGTTGTTGACAAGTACATCG+AGG + chr3.2:77991050-77991069 None:intergenic 45.0%
TAAGCCAACACCATCCTAGT+TGG - chr3.2:77990761-77990780 MS.gene057244:intron 45.0%
TCCCACCGAAATTGACATCA+GGG - chr3.2:77990729-77990748 MS.gene057244:CDS 45.0%
TGACTGTAGGTACATACTCG+GGG + chr3.2:77991073-77991092 None:intergenic 45.0%
TTGGTAGGAGGAGGAGATTT+GGG + chr3.2:77990500-77990519 None:intergenic 45.0%
TTTGGTAGGAGGAGGAGATT+TGG + chr3.2:77990501-77990520 None:intergenic 45.0%
! GCACTGGTCACATTTCCATA+AGG + chr3.2:77991242-77991261 None:intergenic 45.0%
!! ATCAAGAGTCTGGCCTTACT+TGG - chr3.2:77991276-77991295 MS.gene057244:CDS 45.0%
!! CTTTGTTAGCTTTGCCACCA+GGG - chr3.2:77991307-77991326 MS.gene057244:CDS 45.0%
!! TCTGGCCTTACTTGGTTCTT+TGG - chr3.2:77991284-77991303 MS.gene057244:CDS 45.0%
ACTGTAGGTACATACTCGGG+GGG + chr3.2:77991071-77991090 None:intergenic 50.0%
AGAGTTCCTTACGACGTCGA+AGG + chr3.2:77991198-77991217 None:intergenic 50.0%
CCACCGAAATTGACATCAGG+GGG - chr3.2:77990731-77990750 MS.gene057244:CDS 50.0%
CCCACCGAAATTGACATCAG+GGG - chr3.2:77990730-77990749 MS.gene057244:CDS 50.0%
CCCCTGATGTCAATTTCGGT+GGG + chr3.2:77990733-77990752 None:intergenic 50.0%
CCGGACCTGTAGATACAAAC+AGG + chr3.2:77991013-77991032 None:intergenic 50.0%
CCTCCCCCTGATGTCAATTT+CGG + chr3.2:77990737-77990756 None:intergenic 50.0%
CGAAATTGACATCAGGGGGA+GGG - chr3.2:77990735-77990754 MS.gene057244:intron 50.0%
GACTGTAGGTACATACTCGG+GGG + chr3.2:77991072-77991091 None:intergenic 50.0%
! AGGAGGAGATTTGGGTTCAG+AGG + chr3.2:77990492-77990511 None:intergenic 50.0%
! CGATTGCATTTTCGTCAGGC+CGG + chr3.2:77990445-77990464 None:intergenic 50.0%
!! GCTTTGTTAGCTTTGCCACC+AGG - chr3.2:77991306-77991325 MS.gene057244:CDS 50.0%
ACCTCTCCTTCGACGTCGTA+AGG - chr3.2:77991189-77991208 MS.gene057244:CDS 55.0%
AGGTTTCGGTCCAACCCTAG+TGG - chr3.2:77990936-77990955 MS.gene057244:intron 55.0%
CCCCCTGATGTCAATTTCGG+TGG + chr3.2:77990734-77990753 None:intergenic 55.0%
CCGAAATTGACATCAGGGGG+AGG - chr3.2:77990734-77990753 MS.gene057244:CDS 55.0%
GAACTGGTGAACTCACGGTG+TGG - chr3.2:77990401-77990420 MS.gene057244:CDS 55.0%
GAGCGTAGTTTGGTAGGAGG+AGG + chr3.2:77990509-77990528 None:intergenic 55.0%
GCTGAACTCTGCCGTCCTTA+TGG - chr3.2:77991224-77991243 MS.gene057244:CDS 55.0%
GGCTGCGATTGAGAGAGAAC+TGG - chr3.2:77990385-77990404 MS.gene057244:CDS 55.0%
GGTTTCGGTCCAACCCTAGT+GGG - chr3.2:77990937-77990956 MS.gene057244:intron 55.0%
GTGAACTCACGGTGTGGAGA+CGG - chr3.2:77990407-77990426 MS.gene057244:CDS 55.0%
TACGACGTCGAAGGAGAGGT+CGG + chr3.2:77991189-77991208 None:intergenic 55.0%
TCCTTACGACGTCGAAGGAG+AGG + chr3.2:77991193-77991212 None:intergenic 55.0%
! AGCATTCTGAGCATCGTCCC+TGG + chr3.2:77991327-77991346 None:intergenic 55.0%
! ATTCTGAGCATCGTCCCTGG+TGG + chr3.2:77991324-77991343 None:intergenic 55.0%
GAGACGGTCGTGATCTCGAC+CGG - chr3.2:77990423-77990442 MS.gene057244:CDS 60.0%
TTCACCGTCGCTGTCGCTGT+CGG + chr3.2:77990585-77990604 None:intergenic 60.0%
!! TGTCGGTGTCGATGCTGTCG+AGG + chr3.2:77990568-77990587 None:intergenic 60.0%
CGACAGCGACGGTGAACGTG+AGG - chr3.2:77990589-77990608 MS.gene057244:CDS 65.0%
CGATGCTGTCGAGGTCGCTG+TGG + chr3.2:77990559-77990578 None:intergenic 65.0%
GACACCGACAGCGACAGCGA+CGG - chr3.2:77990578-77990597 MS.gene057244:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 77990383 77991397 77990383 ID=MS.gene057244
chr3.2 mRNA 77990383 77991397 77990383 ID=MS.gene057244.t1;Parent=MS.gene057244
chr3.2 exon 77991175 77991397 77991175 ID=MS.gene057244.t1.exon1;Parent=MS.gene057244.t1
chr3.2 CDS 77991175 77991397 77991175 ID=cds.MS.gene057244.t1;Parent=MS.gene057244.t1
chr3.2 exon 77990383 77990753 77990383 ID=MS.gene057244.t1.exon2;Parent=MS.gene057244.t1
chr3.2 CDS 77990383 77990753 77990383 ID=cds.MS.gene057244.t1;Parent=MS.gene057244.t1
Gene Sequence

>MS.gene057244

ATGGCTGCGATTGAGAGAGAACTGGTGAACTCACGGTGTGGAGACGGTCGTGATCTCGACCGGCCTGACGAAAATGCAATCGAGAAGTTTAAGGGCGGCGACGTCTCCTCTGAACCCAAATCTCCTCCTCCTACCAAACTACGCTCATTGTTTTTTGACGAATGGTTGTCGCTCCACAGCGACCTCGACAGCATCGACACCGACAGCGACAGCGACGGTGAACGTGAGGATCTCGCTTTCCCCTTTTTTCCTATTACATTCAATTTTTAATATGCGTTTTAAATTTCCTAAGCAAATTTCAATATTGTTATTGTTATTGTTGAAAACTCGTTAAAGATGGTTTCGATCCCACCGAAATTGACATCAGGGGGAGGGTTCTAAGCCAACACCATCCTAGTTGGCTTTTACTAACCAGTCCAACAATATAATGAATTGAATTTCACTATTATTAACACTAACAAAGTTATTAATTGAATATCCGTTCCGATGTATCACAGACTACAATGAATTGAATTGAATTTCATGTCTATCAATTAATCTCATTTGTATCTACAGGTTTCGGTCCAACCCTAGTGGGTTAATATCACTATTATTAACACTAACAAAGTTATTAATTGAATACCTGTCCCTGTTTGTATCTACAGGTCCGGTTGCAATACATTGTCCTCGATGTACTTGTCAACAACCCCCCGAGTATGTACCTACAGTCAAGATTGGGAGTTTTCACGTACCTAAGTTTATGGAAGCTTTTGTGGATAAGATGATTGGATCCTATCTTGACAACGCTAATTCTGTTCTGGTAACCGACCTCTCCTTCGACGTCGTAAGGAACTCTGATTTGGCTGAACTCTGCCGTCCTTATGGAAATGTGACCAGTGCCGATGTTGCTATTGATCAAGAGTCTGGCCTTACTTGGTTCTTTGGCTTTGTTAGCTTTGCCACCAGGGACGATGCTCAGAATGCTATTAACAATCTCGATGGAGTTCAATGGCTCACCCCGAAAACAACCTCCTAA

Protein sequence

>MS.gene057244.t1

MAAIERELVNSRCGDGRDLDRPDENAIEKFKGGDVSSEPKSPPPTKLRSLFFDEWLSLHSDLDSIDTDSDSDGERPVAIHCPRCTCQQPPEYVPTVKIGSFHVPKFMEAFVDKMIGSYLDNANSVLVTDLSFDVVRNSDLAELCRPYGNVTSADVAIDQESGLTWFFGFVSFATRDDAQNAINNLDGVQWLTPKTTS