Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34936.t1 | RHN42370.1 | 98.9 | 563 | 6 | 0 | 1 | 563 | 1 | 563 | 1.2e-309 | 1071.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34936.t1 | Q8LBH2 | 70.5 | 584 | 138 | 9 | 1 | 563 | 1 | 571 | 5.0e-211 | 735.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34936.t1 | A0A396GMV6 | 98.9 | 563 | 6 | 0 | 1 | 563 | 1 | 563 | 8.4e-310 | 1071.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene34936 | -0.806611 | 7.56E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene34936 | -0.807648 | 4.56E-50 | -1.69E-46 |
| MS.gene049195 | MS.gene34936 | -0.823721 | 1.19E-53 | -1.69E-46 |
| MS.gene049297 | MS.gene34936 | -0.801185 | 1.01E-48 | -1.69E-46 |
| MS.gene049378 | MS.gene34936 | -0.819086 | 1.40E-52 | -1.69E-46 |
| MS.gene049435 | MS.gene34936 | -0.82698 | 2.01E-54 | -1.69E-46 |
| MS.gene049910 | MS.gene34936 | -0.805339 | 1.40E-49 | -1.69E-46 |
| MS.gene049993 | MS.gene34936 | -0.826164 | 3.15E-54 | -1.69E-46 |
| MS.gene050226 | MS.gene34936 | -0.84083 | 6.86E-58 | -1.69E-46 |
| MS.gene050227 | MS.gene34936 | -0.850644 | 1.49E-60 | -1.69E-46 |
| MS.gene050228 | MS.gene34936 | -0.848064 | 7.77E-60 | -1.69E-46 |
| MS.gene050442 | MS.gene34936 | -0.844341 | 8.03E-59 | -1.69E-46 |
| MS.gene050605 | MS.gene34936 | -0.802952 | 4.39E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene34936 | 0.873839 | 1.08E-67 | -1.69E-46 |
| MS.gene050642 | MS.gene34936 | -0.846906 | 1.62E-59 | -1.69E-46 |
| MS.gene050680 | MS.gene34936 | -0.807547 | 4.79E-50 | -1.69E-46 |
| MS.gene050750 | MS.gene34936 | -0.841802 | 3.81E-58 | -1.69E-46 |
| MS.gene050787 | MS.gene34936 | -0.827221 | 1.76E-54 | -1.69E-46 |
| MS.gene050820 | MS.gene34936 | -0.816426 | 5.56E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene34936 | -0.837147 | 6.17E-57 | -1.69E-46 |
| MS.gene051257 | MS.gene34936 | -0.820666 | 6.08E-53 | -1.69E-46 |
| MS.gene051265 | MS.gene34936 | -0.816312 | 5.90E-52 | -1.69E-46 |
| MS.gene051568 | MS.gene34936 | -0.815209 | 1.04E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene34936 | -0.868795 | 5.01E-66 | -1.69E-46 |
| MS.gene051983 | MS.gene34936 | -0.850459 | 1.67E-60 | -1.69E-46 |
| MS.gene052011 | MS.gene34936 | 0.826668 | 2.39E-54 | -1.69E-46 |
| MS.gene052077 | MS.gene34936 | -0.819404 | 1.18E-52 | -1.69E-46 |
| MS.gene052190 | MS.gene34936 | -0.806294 | 8.82E-50 | -1.69E-46 |
| MS.gene052309 | MS.gene34936 | 0.807875 | 4.08E-50 | -1.69E-46 |
| MS.gene052333 | MS.gene34936 | -0.851294 | 9.75E-61 | -1.69E-46 |
| MS.gene052389 | MS.gene34936 | -0.817759 | 2.79E-52 | -1.69E-46 |
| MS.gene053054 | MS.gene34936 | 0.839321 | 1.70E-57 | -1.69E-46 |
| MS.gene053166 | MS.gene34936 | -0.810511 | 1.11E-50 | -1.69E-46 |
| MS.gene053494 | MS.gene34936 | -0.802657 | 5.06E-49 | -1.69E-46 |
| MS.gene053584 | MS.gene34936 | -0.819327 | 1.23E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene34936 | -0.84695 | 1.57E-59 | -1.69E-46 |
| MS.gene054183 | MS.gene34936 | -0.851789 | 7.06E-61 | -1.69E-46 |
| MS.gene054291 | MS.gene34936 | 0.867452 | 1.35E-65 | -1.69E-46 |
| MS.gene054405 | MS.gene34936 | -0.834665 | 2.63E-56 | -1.69E-46 |
| MS.gene054433 | MS.gene34936 | -0.820489 | 6.68E-53 | -1.69E-46 |
| MS.gene054467 | MS.gene34936 | -0.80628 | 8.88E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene34936 | -0.823759 | 1.16E-53 | -1.69E-46 |
| MS.gene054559 | MS.gene34936 | -0.814456 | 1.53E-51 | -1.69E-46 |
| MS.gene054713 | MS.gene34936 | -0.846038 | 2.79E-59 | -1.69E-46 |
| MS.gene054942 | MS.gene34936 | -0.813107 | 3.03E-51 | -1.69E-46 |
| MS.gene054983 | MS.gene34936 | -0.810788 | 9.68E-51 | -1.69E-46 |
| MS.gene05510 | MS.gene34936 | -0.801667 | 8.08E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene34936 | -0.841762 | 3.90E-58 | -1.69E-46 |
| MS.gene055357 | MS.gene34936 | -0.820009 | 8.60E-53 | -1.69E-46 |
| MS.gene055358 | MS.gene34936 | -0.839567 | 1.47E-57 | -1.69E-46 |
| MS.gene055507 | MS.gene34936 | -0.815826 | 7.58E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene34936 | -0.85445 | 1.22E-61 | -1.69E-46 |
| MS.gene056087 | MS.gene34936 | -0.807119 | 5.91E-50 | -1.69E-46 |
| MS.gene056447 | MS.gene34936 | -0.801727 | 7.85E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene34936 | -0.816948 | 4.25E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene34936 | -0.817451 | 3.28E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene34936 | 0.825686 | 4.09E-54 | -1.69E-46 |
| MS.gene056979 | MS.gene34936 | -0.83947 | 1.55E-57 | -1.69E-46 |
| MS.gene057200 | MS.gene34936 | -0.80405 | 2.60E-49 | -1.69E-46 |
| MS.gene057244 | MS.gene34936 | 0.8197 | 1.01E-52 | -1.69E-46 |
| MS.gene057469 | MS.gene34936 | -0.813416 | 2.59E-51 | -1.69E-46 |
| MS.gene057639 | MS.gene34936 | -0.829201 | 5.88E-55 | -1.69E-46 |
| MS.gene058042 | MS.gene34936 | -0.811355 | 7.30E-51 | -1.69E-46 |
| MS.gene058146 | MS.gene34936 | -0.818507 | 1.89E-52 | -1.69E-46 |
| MS.gene05845 | MS.gene34936 | -0.816577 | 5.15E-52 | -1.69E-46 |
| MS.gene058710 | MS.gene34936 | -0.810827 | 9.50E-51 | -1.69E-46 |
| MS.gene059161 | MS.gene34936 | -0.80595 | 1.04E-49 | -1.69E-46 |
| MS.gene059247 | MS.gene34936 | -0.803942 | 2.74E-49 | -1.69E-46 |
| MS.gene059609 | MS.gene34936 | -0.880311 | 6.09E-70 | -1.69E-46 |
| MS.gene05979 | MS.gene34936 | -0.807472 | 4.97E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene34936 | -0.832001 | 1.21E-55 | -1.69E-46 |
| MS.gene060137 | MS.gene34936 | 0.887823 | 1.02E-72 | -1.69E-46 |
| MS.gene060634 | MS.gene34936 | 0.814597 | 1.42E-51 | -1.69E-46 |
| MS.gene060683 | MS.gene34936 | -0.805875 | 1.08E-49 | -1.69E-46 |
| MS.gene060685 | MS.gene34936 | -0.806032 | 1.00E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene34936 | -0.862741 | 4.09E-64 | -1.69E-46 |
| MS.gene060803 | MS.gene34936 | -0.856461 | 3.17E-62 | -1.69E-46 |
| MS.gene061040 | MS.gene34936 | 0.843728 | 1.17E-58 | -1.69E-46 |
| MS.gene061114 | MS.gene34936 | 0.910891 | 1.13E-82 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene34936 | MS.gene65381 | PPI |
| MS.gene34936 | MS.gene036713 | PPI |
| MS.gene34936 | MS.gene77001 | PPI |
| MS.gene58034 | MS.gene34936 | PPI |
| MS.gene34936 | MS.gene66443 | PPI |
| MS.gene58033 | MS.gene34936 | PPI |
| MS.gene24461 | MS.gene34936 | PPI |
| MS.gene75800 | MS.gene34936 | PPI |
| MS.gene074497 | MS.gene34936 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34936.t1 | MTR_4g127440 | 54.610 | 564 | 223 | 9 | 1 | 537 | 1 | 558 | 0.0 | 564 |
| MS.gene34936.t1 | MTR_2g037980 | 75.000 | 316 | 79 | 0 | 1 | 316 | 1 | 316 | 4.78e-178 | 515 |
| MS.gene34936.t1 | MTR_4g115420 | 49.912 | 567 | 222 | 10 | 2 | 535 | 4 | 541 | 1.32e-171 | 497 |
| MS.gene34936.t1 | MTR_0530s0020 | 39.194 | 546 | 256 | 16 | 21 | 535 | 6 | 506 | 3.83e-110 | 339 |
| MS.gene34936.t1 | MTR_4g114960 | 31.317 | 562 | 299 | 16 | 8 | 506 | 7 | 544 | 3.80e-77 | 255 |
| MS.gene34936.t1 | MTR_8g008860 | 32.593 | 540 | 311 | 15 | 8 | 504 | 7 | 536 | 5.99e-68 | 230 |
| MS.gene34936.t1 | MTR_2g008170 | 38.393 | 336 | 167 | 6 | 6 | 309 | 5 | 332 | 2.82e-64 | 221 |
| MS.gene34936.t1 | MTR_3g080490 | 34.783 | 322 | 194 | 6 | 8 | 322 | 6 | 318 | 2.20e-61 | 218 |
| MS.gene34936.t1 | MTR_3g106900 | 48.052 | 154 | 76 | 3 | 3 | 154 | 2 | 153 | 6.86e-37 | 145 |
| MS.gene34936.t1 | MTR_3g106900 | 34.877 | 367 | 185 | 11 | 181 | 506 | 227 | 580 | 2.55e-27 | 117 |
| MS.gene34936.t1 | MTR_8g106040 | 28.803 | 309 | 205 | 8 | 7 | 307 | 5 | 306 | 1.19e-35 | 139 |
| MS.gene34936.t1 | MTR_6g059380 | 25.000 | 364 | 220 | 12 | 8 | 344 | 10 | 347 | 1.80e-23 | 104 |
| MS.gene34936.t1 | MTR_1g033880 | 25.076 | 331 | 206 | 12 | 1 | 319 | 1 | 301 | 2.81e-15 | 78.2 |
| MS.gene34936.t1 | MTR_3g086670 | 33.548 | 155 | 96 | 4 | 8 | 159 | 6 | 156 | 7.15e-15 | 76.3 |
| MS.gene34936.t1 | MTR_8g089850 | 30.178 | 169 | 106 | 5 | 34 | 199 | 31 | 190 | 3.80e-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34936.t1 | AT2G01600 | 70.719 | 584 | 137 | 9 | 1 | 563 | 1 | 571 | 0.0 | 786 |
| MS.gene34936.t1 | AT1G14910 | 69.381 | 565 | 153 | 8 | 1 | 559 | 1 | 551 | 0.0 | 769 |
| MS.gene34936.t1 | AT1G14910 | 68.496 | 565 | 153 | 9 | 1 | 559 | 1 | 546 | 0.0 | 750 |
| MS.gene34936.t1 | AT4G25940 | 57.383 | 535 | 184 | 9 | 1 | 498 | 1 | 528 | 0.0 | 566 |
| MS.gene34936.t1 | AT5G57200 | 76.266 | 316 | 74 | 1 | 1 | 316 | 1 | 315 | 3.45e-179 | 518 |
| MS.gene34936.t1 | AT5G35200 | 51.351 | 518 | 202 | 11 | 8 | 511 | 12 | 493 | 1.77e-164 | 479 |
| MS.gene34936.t1 | AT5G35200 | 51.351 | 518 | 202 | 11 | 8 | 511 | 12 | 493 | 1.77e-164 | 479 |
| MS.gene34936.t1 | AT1G03050 | 34.483 | 580 | 286 | 21 | 1 | 506 | 1 | 560 | 2.42e-77 | 256 |
| MS.gene34936.t1 | AT4G32285 | 32.203 | 590 | 295 | 15 | 6 | 506 | 4 | 577 | 1.16e-73 | 247 |
| MS.gene34936.t1 | AT4G32285 | 32.203 | 590 | 295 | 15 | 6 | 506 | 4 | 577 | 1.16e-73 | 247 |
| MS.gene34936.t1 | AT4G02650 | 32.000 | 575 | 296 | 19 | 8 | 506 | 7 | 562 | 6.64e-71 | 239 |
| MS.gene34936.t1 | AT1G05020 | 34.969 | 326 | 185 | 6 | 8 | 309 | 6 | 328 | 4.43e-58 | 205 |
| MS.gene34936.t1 | AT2G25430 | 51.007 | 149 | 69 | 3 | 6 | 152 | 4 | 150 | 7.17e-39 | 151 |
| MS.gene34936.t1 | AT2G25430 | 30.914 | 372 | 189 | 10 | 181 | 506 | 247 | 596 | 2.07e-28 | 120 |
| MS.gene34936.t1 | AT1G33340 | 27.687 | 307 | 200 | 7 | 7 | 305 | 9 | 301 | 5.61e-29 | 119 |
| MS.gene34936.t1 | AT4G40080 | 32.367 | 207 | 127 | 6 | 1 | 199 | 1 | 202 | 1.68e-21 | 97.1 |
| MS.gene34936.t1 | AT1G25240 | 29.012 | 324 | 183 | 11 | 4 | 315 | 2 | 290 | 2.73e-21 | 96.3 |
| MS.gene34936.t1 | AT1G25240 | 29.012 | 324 | 183 | 11 | 4 | 315 | 1 | 289 | 2.80e-21 | 96.3 |
| MS.gene34936.t1 | AT1G68110 | 25.079 | 315 | 202 | 10 | 4 | 307 | 1 | 292 | 4.83e-18 | 86.7 |
| MS.gene34936.t1 | AT1G14686 | 24.684 | 316 | 194 | 12 | 4 | 310 | 1 | 281 | 3.08e-11 | 65.5 |
Find 160 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTTTGCGGATTCAGGATT+TGG | 0.185619 | 4.1:+16592247 | MS.gene34936:CDS |
| GCAAATCCCTTTGCGGATTC+AGG | 0.201574 | 4.1:+16592241 | MS.gene34936:CDS |
| ACTTCTTAGATGTCTTTCTT+TGG | 0.204881 | 4.1:-16585546 | None:intergenic |
| GCTGCACAACAACCAGTTTA+TGG | 0.213469 | 4.1:+16592043 | MS.gene34936:CDS |
| CTGCTGACCCGCACGCTTAT+AGG | 0.229577 | 4.1:-16590621 | None:intergenic |
| TCCTGTCCTTGGGCAGGTTT+GGG | 0.249846 | 4.1:-16587485 | None:intergenic |
| CTCCTGTCCTTGGGCAGGTT+TGG | 0.252819 | 4.1:-16587486 | None:intergenic |
| CCTTTGCGGATTCAGGATTT+GGG | 0.252980 | 4.1:+16592248 | MS.gene34936:CDS |
| TCATGAACTTCAAATGGATT+TGG | 0.265369 | 4.1:-16592076 | None:intergenic |
| CTTCTTAGATGTCTTTCTTT+GGG | 0.274846 | 4.1:-16585545 | None:intergenic |
| GCCATAGTCTCCACTGAAAA+TGG | 0.279408 | 4.1:+16591397 | MS.gene34936:CDS |
| CATGAACTTCAAATGGATTT+GGG | 0.280536 | 4.1:-16592075 | None:intergenic |
| TTCTGATGGCACCTGTTCTT+CGG | 0.286225 | 4.1:-16591147 | None:intergenic |
| GACTTGCTAAGACGCGAAAT+TGG | 0.292068 | 4.1:+16585766 | MS.gene34936:CDS |
| TTTAGATAGTGAGGAGCTAT+TGG | 0.306314 | 4.1:+16588509 | MS.gene34936:CDS |
| CAACTATGGAAGAGTACATT+AGG | 0.314951 | 4.1:+16590937 | MS.gene34936:CDS |
| CAGTCTTGGCGAAGAGTTTA+CGG | 0.318642 | 4.1:+16585332 | MS.gene34936:CDS |
| TTGCTAACAGCTGCTCCTTC+TGG | 0.320696 | 4.1:-16588716 | None:intergenic |
| TCAATTGCAAGAACTTCTTC+TGG | 0.323093 | 4.1:-16591110 | None:intergenic |
| GGAGGTGCAGGCTCAGAATT+GGG | 0.326530 | 4.1:-16591194 | None:intergenic |
| AAGAGCATTTCTTTCCTCTA+TGG | 0.329697 | 4.1:-16591368 | None:intergenic |
| TCTGATGGCACCTGTTCTTC+GGG | 0.330942 | 4.1:-16591146 | None:intergenic |
| TGCCATTTGGACAGCGGCCG+GGG | 0.331631 | 4.1:-16592128 | None:intergenic |
| AAGAAAGACTTGAATGCTTT+CGG | 0.338922 | 4.1:+16587429 | MS.gene34936:CDS |
| GAATTACCTTCTCCTGTCCT+TGG | 0.343652 | 4.1:-16587496 | None:intergenic |
| CAACTTCTGCTGTTCGTCCC+AGG | 0.346336 | 4.1:+16585706 | MS.gene34936:CDS |
| GCTGTTGCATTCCTGCCATT+TGG | 0.350069 | 4.1:-16592141 | None:intergenic |
| TCCTCTATGGATGATGCATT+AGG | 0.359288 | 4.1:-16591355 | None:intergenic |
| GCAAGTCTCCAGTGTCAAAA+TGG | 0.362620 | 4.1:-16591238 | None:intergenic |
| CAGCCTGGGATTGTTCTGCC+TGG | 0.365265 | 4.1:+16587381 | MS.gene34936:intron |
| TTATTATGTGGGTGAGAAAC+GGG | 0.370777 | 4.1:-16592286 | None:intergenic |
| AGGAGGTGCAGGCTCAGAAT+TGG | 0.371133 | 4.1:-16591195 | None:intergenic |
| GCTGCTGATGAGGCTGAGGC+TGG | 0.373860 | 4.1:-16592195 | None:intergenic |
| AACTTTGGTGGTATCTTTGA+CGG | 0.380674 | 4.1:-16585357 | None:intergenic |
| GGGGCTGGTGCTCCATAAAC+TGG | 0.381353 | 4.1:-16592055 | None:intergenic |
| CATCCTTAAAATTTGAAAGT+TGG | 0.385074 | 4.1:-16587202 | None:intergenic |
| CAGTACTCGGAGTGCTGACC+AGG | 0.388662 | 4.1:-16591589 | None:intergenic |
| TGGATGATGCATTAGGTTCC+AGG | 0.391284 | 4.1:-16591348 | None:intergenic |
| GGTGGTGGAGGAGGAGGTGC+AGG | 0.394378 | 4.1:-16591206 | None:intergenic |
| TGAGATGGTGGTGGAGGAGG+AGG | 0.402344 | 4.1:-16591212 | None:intergenic |
| CCACATAATAACAATCCATT+TGG | 0.402689 | 4.1:+16592298 | MS.gene34936:CDS |
| CCTGCCATTTGGACAGCGGC+CGG | 0.404720 | 4.1:-16592130 | None:intergenic |
| TGTCAAAGAGCCCGAAGAAC+AGG | 0.409894 | 4.1:+16591136 | MS.gene34936:CDS |
| CTATGAAGTTTGCAAAGGAT+TGG | 0.410819 | 4.1:+16590757 | MS.gene34936:CDS |
| AATGGATCATGAACTTCAAA+TGG | 0.420594 | 4.1:-16592082 | None:intergenic |
| TATATCGACTTGTTGGCTGC+CGG | 0.425212 | 4.1:+16588559 | MS.gene34936:CDS |
| AATTACCTTCTCCTGTCCTT+GGG | 0.427574 | 4.1:-16587495 | None:intergenic |
| GCAGCAGCAGCATATGTTGA+TGG | 0.428102 | 4.1:+16592213 | MS.gene34936:CDS |
| CCGCACGCTTATAGGCTTCA+AGG | 0.429737 | 4.1:-16590613 | None:intergenic |
| GAATCCGCAAAGGGATTTGC+AGG | 0.431243 | 4.1:-16592238 | None:intergenic |
| ACCTTCTCCTGTCCTTGGGC+AGG | 0.431535 | 4.1:-16587491 | None:intergenic |
| TTGTGAGATGGTGGTGGAGG+AGG | 0.437159 | 4.1:-16591215 | None:intergenic |
| TACTGGATGGGAGCTTGCCC+TGG | 0.437527 | 4.1:+16591571 | MS.gene34936:CDS |
| TGGACATTCAACGTGATTAG+TGG | 0.438730 | 4.1:-16585522 | None:intergenic |
| GGAATGTCAGGCTGGTGGAC+TGG | 0.439998 | 4.1:+16591976 | MS.gene34936:intron |
| TGCTGCTGCTGATGAGGCTG+AGG | 0.440595 | 4.1:-16592199 | None:intergenic |
| CTGCCATTTGGACAGCGGCC+GGG | 0.440665 | 4.1:-16592129 | None:intergenic |
| CAGCTTCTATATCGACTTGT+TGG | 0.441624 | 4.1:+16588552 | MS.gene34936:CDS |
| AGCCTGGGATTGTTCTGCCT+GGG | 0.446845 | 4.1:+16587382 | MS.gene34936:intron |
| CAACAGCAAGCGAATCCATT+TGG | 0.449659 | 4.1:+16592157 | MS.gene34936:CDS |
| GCTTTAATAGCTTCATGTCT+TGG | 0.453459 | 4.1:-16590590 | None:intergenic |
| ACAAAAGATTTCGATCCTAC+TGG | 0.455678 | 4.1:+16591554 | MS.gene34936:CDS |
| AACAAGCCGGTGCTTCCAAA+TGG | 0.457190 | 4.1:-16592313 | None:intergenic |
| AACTTCTGCTGTTCGTCCCA+GGG | 0.458247 | 4.1:+16585707 | MS.gene34936:CDS |
| ATTTACTGCGCTATTAATGA+TGG | 0.459826 | 4.1:+16590450 | MS.gene34936:CDS |
| GTACTAACCATCGGCTCACT+TGG | 0.460104 | 4.1:-16590984 | None:intergenic |
| CACCCTCCCTGAGTAACCTA+TGG | 0.461427 | 4.1:-16587127 | None:intergenic |
| CCAAATGGATTGTTATTATG+TGG | 0.463425 | 4.1:-16592298 | None:intergenic |
| GTTATTATGTGGGTGAGAAA+CGG | 0.463914 | 4.1:-16592287 | None:intergenic |
| AACAATCCATTTGGAAGCAC+CGG | 0.464105 | 4.1:+16592307 | MS.gene34936:CDS |
| AACTGTGGGTAAGGACCAAA+TGG | 0.466710 | 4.1:-16592172 | None:intergenic |
| ATATCGACTTGTTGGCTGCC+GGG | 0.467584 | 4.1:+16588560 | MS.gene34936:CDS |
| GCAAAGGATTGGAACTTGCT+AGG | 0.473319 | 4.1:+16590768 | MS.gene34936:CDS |
| CTATAAGCGTGCGGGTCAGC+AGG | 0.476852 | 4.1:+16590622 | MS.gene34936:CDS |
| GTACATTAGGGAGGCACCCC+GGG | 0.477247 | 4.1:+16590950 | MS.gene34936:CDS |
| GACATGGGCGAGACCAACTT+TGG | 0.477335 | 4.1:-16585372 | None:intergenic |
| TTCAGTCAATGAGAGACAAT+TGG | 0.480704 | 4.1:+16591619 | MS.gene34936:CDS |
| ATGTTGCTTGATATAGCAAA+TGG | 0.483557 | 4.1:-16592100 | None:intergenic |
| ATCAAGCAACATTGCTCCCC+CGG | 0.485362 | 4.1:+16592111 | MS.gene34936:CDS |
| CTTTGCGGATTCAGGATTTG+GGG | 0.488384 | 4.1:+16592249 | MS.gene34936:CDS |
| CACTTGGAACATTAACAACC+CGG | 0.489997 | 4.1:-16590968 | None:intergenic |
| AAGGATGACTCTAGTCCAAT+CGG | 0.491603 | 4.1:+16587218 | MS.gene34936:CDS |
| CTTAACAGCTTATATGATGA+AGG | 0.493587 | 4.1:+16592010 | MS.gene34936:CDS |
| TGAGGCTGGAACTGTGGGTA+AGG | 0.498922 | 4.1:-16592181 | None:intergenic |
| AACTATGGAAGAGTACATTA+GGG | 0.503486 | 4.1:+16590938 | MS.gene34936:CDS |
| CTTAGATGTCTTTCTTTGGG+TGG | 0.506041 | 4.1:-16585542 | None:intergenic |
| GGACCCTGCAAATCCCTTTG+CGG | 0.509785 | 4.1:+16592234 | MS.gene34936:CDS |
| TATAGAAGCTGTTGTAAAGC+AGG | 0.509890 | 4.1:-16588540 | None:intergenic |
| AGAATTTCCTCTCTAAGCGT+TGG | 0.511778 | 4.1:-16587152 | None:intergenic |
| TAGTCATCCATAGGTTACTC+AGG | 0.518273 | 4.1:+16587120 | MS.gene34936:CDS |
| CCGCGTAATCGCTATTGACA+TGG | 0.523311 | 4.1:-16585388 | None:intergenic |
| CGCACGCTTATAGGCTTCAA+GGG | 0.523909 | 4.1:-16590612 | None:intergenic |
| ATATGCTGCTGCTGCTGATG+AGG | 0.524715 | 4.1:-16592205 | None:intergenic |
| ACCCAAACCTGCCCAAGGAC+AGG | 0.527781 | 4.1:+16587484 | MS.gene34936:CDS |
| CATTATCAATCTTGTTGACA+AGG | 0.528067 | 4.1:+16590473 | MS.gene34936:CDS |
| ACAATAAGCAACATCAGCCC+TGG | 0.528722 | 4.1:-16585724 | None:intergenic |
| CCGGCCGCTGTCCAAATGGC+AGG | 0.532125 | 4.1:+16592130 | MS.gene34936:CDS |
| TGGTTGTGAGATGGTGGTGG+AGG | 0.535548 | 4.1:-16591218 | None:intergenic |
| TGCTAAGACGCGAAATTGGA+CGG | 0.536350 | 4.1:+16585770 | MS.gene34936:CDS |
| CAATGGGTACTCTTCAGTCT+TGG | 0.538882 | 4.1:+16585318 | None:intergenic |
| CCAAATCCTGAATCCGCAAA+GGG | 0.540155 | 4.1:-16592247 | None:intergenic |
| AGCAGGGACACAGCAGGACT+AGG | 0.541151 | 4.1:+16588484 | MS.gene34936:intron |
| ACTTCAAATGGATTTGGGGC+TGG | 0.546087 | 4.1:-16592070 | None:intergenic |
| CATTCCTGCCATTTGGACAG+CGG | 0.548232 | 4.1:-16592134 | None:intergenic |
| GCCATTTGGACAGCGGCCGG+GGG | 0.549447 | 4.1:-16592127 | None:intergenic |
| GATACAATATGCGCTAGCTC+TGG | 0.549765 | 4.1:+16588745 | MS.gene34936:CDS |
| TTATTATCACTAGGTTCTGA+TGG | 0.551694 | 4.1:-16591161 | None:intergenic |
| TCTTATTTCTTACAACAAGC+CGG | 0.553829 | 4.1:-16592326 | None:intergenic |
| AAGATTTCGATCCTACTGGA+TGG | 0.558420 | 4.1:+16591558 | MS.gene34936:CDS |
| GCAGGGACACAGCAGGACTA+GGG | 0.563205 | 4.1:+16588485 | MS.gene34936:intron |
| GTCAAAGATACCACCAAAGT+TGG | 0.563537 | 4.1:+16585359 | MS.gene34936:CDS |
| ACCTAATGCATCATCCATAG+AGG | 0.564544 | 4.1:+16591354 | MS.gene34936:CDS |
| AAATGGTTGTGAGATGGTGG+TGG | 0.566222 | 4.1:-16591221 | None:intergenic |
| TGTGAATATAGGCACTGCAT+CGG | 0.567918 | 4.1:+16591508 | MS.gene34936:intron |
| CAAATGGATTGTTATTATGT+GGG | 0.570554 | 4.1:-16592297 | None:intergenic |
| AGACATACCTCTCTTAAGAC+AGG | 0.571173 | 4.1:-16590803 | None:intergenic |
| TAATGTTCCAAGTGAGCCGA+TGG | 0.572868 | 4.1:+16590977 | MS.gene34936:CDS |
| TGAGGCTGAGGCTGGAACTG+TGG | 0.573458 | 4.1:-16592187 | None:intergenic |
| GAGGTGCAGGCTCAGAATTG+GGG | 0.576097 | 4.1:-16591193 | None:intergenic |
| GATTTCTATGAAGTTTGCAA+AGG | 0.576897 | 4.1:+16590752 | MS.gene34936:CDS |
| AGGTGCAGGCTCAGAATTGG+GGG | 0.577902 | 4.1:-16591192 | None:intergenic |
| GGAAAGAAATGCTCTTGCGC+TGG | 0.578170 | 4.1:+16591375 | MS.gene34936:CDS |
| TCAGTTTCCTGTCTTAAGAG+AGG | 0.581783 | 4.1:+16590796 | MS.gene34936:CDS |
| ACCTGCCCAAGGACAGGAGA+AGG | 0.586183 | 4.1:+16587490 | MS.gene34936:CDS |
| CCCAAATCCTGAATCCGCAA+AGG | 0.589248 | 4.1:-16592248 | None:intergenic |
| ATGGGCGAGACCAACTTTGG+TGG | 0.590062 | 4.1:-16585369 | None:intergenic |
| AAACACTGATAGTCATCCAT+AGG | 0.590218 | 4.1:+16587111 | MS.gene34936:CDS |
| GTCATGGAATGTCAGGCTGG+TGG | 0.590289 | 4.1:+16591971 | MS.gene34936:intron |
| AGTACATTAGGGAGGCACCC+CGG | 0.591792 | 4.1:+16590949 | MS.gene34936:CDS |
| TACCCAGGCAGAACAATCCC+AGG | 0.597737 | 4.1:-16587384 | None:intergenic |
| GACTAGGGATTTAGATAGTG+AGG | 0.598908 | 4.1:+16588500 | MS.gene34936:CDS |
| ACTTGGAACATTAACAACCC+GGG | 0.599939 | 4.1:-16590967 | None:intergenic |
| ATCCATAGGTTACTCAGGGA+GGG | 0.600254 | 4.1:+16587125 | MS.gene34936:CDS |
| CTTGAAGCCTATAAGCGTGC+GGG | 0.602046 | 4.1:+16590614 | MS.gene34936:CDS |
| AGATTTCGATCCTACTGGAT+GGG | 0.602139 | 4.1:+16591559 | MS.gene34936:CDS |
| ATGAACTTCAAATGGATTTG+GGG | 0.604886 | 4.1:-16592074 | None:intergenic |
| GAGGCTGAGGCTGGAACTGT+GGG | 0.606106 | 4.1:-16592186 | None:intergenic |
| CAATAAGCAACATCAGCCCT+GGG | 0.614387 | 4.1:-16585723 | None:intergenic |
| TCAAAATGGTTGTGAGATGG+TGG | 0.614778 | 4.1:-16591224 | None:intergenic |
| TTTGCGGATTCAGGATTTGG+GGG | 0.625862 | 4.1:+16592250 | MS.gene34936:CDS |
| CGCGTAATCGCTATTGACAT+GGG | 0.631037 | 4.1:-16585387 | None:intergenic |
| AGTCATCCATAGGTTACTCA+GGG | 0.631167 | 4.1:+16587121 | MS.gene34936:CDS |
| TATGTAGGGATTGAATGACC+TGG | 0.635485 | 4.1:+16591330 | MS.gene34936:intron |
| GGGTGATCCAACGCTTAGAG+AGG | 0.638082 | 4.1:+16587145 | MS.gene34936:CDS |
| CCTTGAAGCCTATAAGCGTG+CGG | 0.638815 | 4.1:+16590613 | MS.gene34936:CDS |
| CGTCTACCCAAACCTGCCCA+AGG | 0.639108 | 4.1:+16587479 | MS.gene34936:CDS |
| ACTGAAGAAATATCAGTACT+CGG | 0.639433 | 4.1:-16591602 | None:intergenic |
| CATCCATAGGTTACTCAGGG+AGG | 0.645447 | 4.1:+16587124 | MS.gene34936:CDS |
| AGGGCAAGCTCCCATCCAGT+AGG | 0.647054 | 4.1:-16591569 | None:intergenic |
| TAATGCATAGGTACGTACCC+AGG | 0.647422 | 4.1:-16587399 | None:intergenic |
| CTATAAATTACACAGCCAGA+AGG | 0.652759 | 4.1:+16588701 | MS.gene34936:intron |
| CTTGGAACATTAACAACCCG+GGG | 0.656680 | 4.1:-16590966 | None:intergenic |
| GTGTCAAAATGGTTGTGAGA+TGG | 0.662795 | 4.1:-16591227 | None:intergenic |
| AATCCGCAAAGGGATTTGCA+GGG | 0.665370 | 4.1:-16592237 | None:intergenic |
| GTTGTAAGAAAAGATTGCGG+AGG | 0.668024 | 4.1:-16590918 | None:intergenic |
| GCAGCAACATTATTATCACT+AGG | 0.687838 | 4.1:-16591170 | None:intergenic |
| TATGGAAGAGTACATTAGGG+AGG | 0.705602 | 4.1:+16590941 | MS.gene34936:CDS |
| AGTACTCGGAGTGCTGACCA+GGG | 0.708772 | 4.1:-16591588 | None:intergenic |
| CCATGTCAATAGCGATTACG+CGG | 0.714918 | 4.1:+16585388 | MS.gene34936:CDS |
| ATAGTTGTAAGAAAAGATTG+CGG | 0.742982 | 4.1:-16590921 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 16585320 | 16592339 | 16585320 | ID=MS.gene34936 |
| chr4.1 | mRNA | 16585320 | 16592339 | 16585320 | ID=MS.gene34936.t1;Parent=MS.gene34936 |
| chr4.1 | exon | 16585320 | 16585409 | 16585320 | ID=MS.gene34936.t1.exon1;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16585320 | 16585409 | 16585320 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16585497 | 16585566 | 16585497 | ID=MS.gene34936.t1.exon2;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16585497 | 16585566 | 16585497 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16585694 | 16585791 | 16585694 | ID=MS.gene34936.t1.exon3;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16585694 | 16585791 | 16585694 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16587101 | 16587239 | 16587101 | ID=MS.gene34936.t1.exon4;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16587101 | 16587239 | 16587101 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16587384 | 16587511 | 16587384 | ID=MS.gene34936.t1.exon5;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16587384 | 16587511 | 16587384 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16588489 | 16588581 | 16588489 | ID=MS.gene34936.t1.exon6;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16588489 | 16588581 | 16588489 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16588716 | 16588766 | 16588716 | ID=MS.gene34936.t1.exon7;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16588716 | 16588766 | 16588716 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16590429 | 16590494 | 16590429 | ID=MS.gene34936.t1.exon8;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16590429 | 16590494 | 16590429 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16590578 | 16590643 | 16590578 | ID=MS.gene34936.t1.exon9;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16590578 | 16590643 | 16590578 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16590737 | 16590817 | 16590737 | ID=MS.gene34936.t1.exon10;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16590737 | 16590817 | 16590737 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16590918 | 16590998 | 16590918 | ID=MS.gene34936.t1.exon11;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16590918 | 16590998 | 16590918 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16591092 | 16591262 | 16591092 | ID=MS.gene34936.t1.exon12;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16591092 | 16591262 | 16591092 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16591337 | 16591418 | 16591337 | ID=MS.gene34936.t1.exon13;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16591337 | 16591418 | 16591337 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16591519 | 16591640 | 16591519 | ID=MS.gene34936.t1.exon14;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16591519 | 16591640 | 16591519 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| chr4.1 | exon | 16591986 | 16592339 | 16591986 | ID=MS.gene34936.t1.exon15;Parent=MS.gene34936.t1 |
| chr4.1 | CDS | 16591986 | 16592339 | 16591986 | ID=cds.MS.gene34936.t1;Parent=MS.gene34936.t1 |
| Gene Sequence |
| Protein sequence |
