Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08324.t1 | RHN42370.1 | 98.9 | 563 | 6 | 0 | 1 | 563 | 1 | 563 | 1.2e-309 | 1071.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08324.t1 | Q8LBH2 | 70.5 | 584 | 138 | 9 | 1 | 563 | 1 | 571 | 5.0e-211 | 735.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08324.t1 | A0A396GMV6 | 98.9 | 563 | 6 | 0 | 1 | 563 | 1 | 563 | 8.4e-310 | 1071.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049195 | MS.gene08324 | -0.824249 | 8.94E-54 | -1.69E-46 |
| MS.gene049297 | MS.gene08324 | -0.811751 | 5.98E-51 | -1.69E-46 |
| MS.gene04933 | MS.gene08324 | -0.812634 | 3.84E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene08324 | -0.824761 | 6.78E-54 | -1.69E-46 |
| MS.gene049435 | MS.gene08324 | -0.820393 | 7.02E-53 | -1.69E-46 |
| MS.gene049993 | MS.gene08324 | -0.808723 | 2.69E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene08324 | -0.824436 | 8.08E-54 | -1.69E-46 |
| MS.gene050227 | MS.gene08324 | -0.830086 | 3.58E-55 | -1.69E-46 |
| MS.gene050228 | MS.gene08324 | -0.831783 | 1.37E-55 | -1.69E-46 |
| MS.gene050303 | MS.gene08324 | -0.803725 | 3.04E-49 | -1.69E-46 |
| MS.gene050319 | MS.gene08324 | 0.808016 | 3.81E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene08324 | -0.850431 | 1.71E-60 | -1.69E-46 |
| MS.gene050605 | MS.gene08324 | -0.802054 | 6.73E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene08324 | 0.880536 | 5.06E-70 | -1.69E-46 |
| MS.gene050642 | MS.gene08324 | -0.843347 | 1.48E-58 | -1.69E-46 |
| MS.gene050750 | MS.gene08324 | -0.851911 | 6.52E-61 | -1.69E-46 |
| MS.gene050787 | MS.gene08324 | -0.818087 | 2.35E-52 | -1.69E-46 |
| MS.gene050820 | MS.gene08324 | -0.80426 | 2.35E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene08324 | -0.839337 | 1.68E-57 | -1.69E-46 |
| MS.gene051265 | MS.gene08324 | -0.809293 | 2.03E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene08324 | -0.82758 | 1.45E-54 | -1.69E-46 |
| MS.gene051983 | MS.gene08324 | -0.841345 | 5.03E-58 | -1.69E-46 |
| MS.gene052176 | MS.gene08324 | -0.816471 | 5.44E-52 | -1.69E-46 |
| MS.gene052309 | MS.gene08324 | 0.830694 | 2.54E-55 | -1.69E-46 |
| MS.gene052333 | MS.gene08324 | -0.830085 | 3.58E-55 | -1.69E-46 |
| MS.gene052389 | MS.gene08324 | -0.812629 | 3.85E-51 | -1.69E-46 |
| MS.gene052867 | MS.gene08324 | -0.804711 | 1.89E-49 | -1.69E-46 |
| MS.gene053054 | MS.gene08324 | 0.83287 | 7.39E-56 | -1.69E-46 |
| MS.gene053166 | MS.gene08324 | -0.802671 | 5.02E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene08324 | -0.837831 | 4.12E-57 | -1.69E-46 |
| MS.gene05414 | MS.gene08324 | -0.823129 | 1.64E-53 | -1.69E-46 |
| MS.gene054183 | MS.gene08324 | -0.828918 | 6.88E-55 | -1.69E-46 |
| MS.gene054291 | MS.gene08324 | 0.869943 | 2.12E-66 | -1.69E-46 |
| MS.gene054405 | MS.gene08324 | -0.835863 | 1.31E-56 | -1.69E-46 |
| MS.gene054433 | MS.gene08324 | -0.830022 | 3.71E-55 | -1.69E-46 |
| MS.gene054468 | MS.gene08324 | -0.818448 | 1.95E-52 | -1.69E-46 |
| MS.gene054713 | MS.gene08324 | -0.813907 | 2.02E-51 | -1.69E-46 |
| MS.gene054942 | MS.gene08324 | -0.818638 | 1.77E-52 | -1.69E-46 |
| MS.gene054983 | MS.gene08324 | -0.801711 | 7.92E-49 | -1.69E-46 |
| MS.gene055166 | MS.gene08324 | -0.81006 | 1.39E-50 | -1.69E-46 |
| MS.gene055356 | MS.gene08324 | -0.837299 | 5.64E-57 | -1.69E-46 |
| MS.gene055357 | MS.gene08324 | -0.812261 | 4.63E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene08324 | -0.845812 | 3.21E-59 | -1.69E-46 |
| MS.gene055701 | MS.gene08324 | -0.838564 | 2.67E-57 | -1.69E-46 |
| MS.gene056087 | MS.gene08324 | -0.819206 | 1.31E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene08324 | -0.822571 | 2.21E-53 | -1.69E-46 |
| MS.gene056899 | MS.gene08324 | 0.816375 | 5.71E-52 | -1.69E-46 |
| MS.gene056979 | MS.gene08324 | -0.833209 | 6.08E-56 | -1.69E-46 |
| MS.gene057060 | MS.gene08324 | -0.821333 | 4.27E-53 | -1.69E-46 |
| MS.gene057200 | MS.gene08324 | -0.804972 | 1.67E-49 | -1.69E-46 |
| MS.gene057244 | MS.gene08324 | 0.800967 | 1.12E-48 | -1.69E-46 |
| MS.gene05761 | MS.gene08324 | -0.80384 | 2.88E-49 | -1.69E-46 |
| MS.gene057639 | MS.gene08324 | -0.810164 | 1.32E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene08324 | -0.80518 | 1.51E-49 | -1.69E-46 |
| MS.gene058146 | MS.gene08324 | -0.82243 | 2.38E-53 | -1.69E-46 |
| MS.gene05845 | MS.gene08324 | -0.819281 | 1.26E-52 | -1.69E-46 |
| MS.gene058549 | MS.gene08324 | -0.801404 | 9.15E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene08324 | -0.806905 | 6.55E-50 | -1.69E-46 |
| MS.gene059161 | MS.gene08324 | -0.8015 | 8.74E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene08324 | -0.812152 | 4.89E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene08324 | -0.808226 | 3.44E-50 | -1.69E-46 |
| MS.gene059609 | MS.gene08324 | -0.854562 | 1.13E-61 | -1.69E-46 |
| MS.gene05979 | MS.gene08324 | -0.823188 | 1.58E-53 | -1.69E-46 |
| MS.gene059849 | MS.gene08324 | 0.806715 | 7.19E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene08324 | -0.809146 | 2.19E-50 | -1.69E-46 |
| MS.gene059896 | MS.gene08324 | 0.800235 | 1.59E-48 | -1.69E-46 |
| MS.gene060137 | MS.gene08324 | 0.880881 | 3.81E-70 | -1.69E-46 |
| MS.gene060322 | MS.gene08324 | -0.826427 | 2.73E-54 | -1.69E-46 |
| MS.gene060634 | MS.gene08324 | 0.835578 | 1.55E-56 | -1.69E-46 |
| MS.gene060683 | MS.gene08324 | -0.812816 | 3.51E-51 | -1.69E-46 |
| MS.gene060685 | MS.gene08324 | -0.813141 | 2.97E-51 | -1.69E-46 |
| MS.gene060802 | MS.gene08324 | -0.87563 | 2.65E-68 | -1.69E-46 |
| MS.gene060803 | MS.gene08324 | -0.867452 | 1.35E-65 | -1.69E-46 |
| MS.gene060998 | MS.gene08324 | -0.800389 | 1.47E-48 | -1.69E-46 |
| MS.gene061040 | MS.gene08324 | 0.869553 | 2.84E-66 | -1.69E-46 |
| MS.gene061114 | MS.gene08324 | 0.894256 | 2.93E-75 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08324 | MS.gene77001 | PPI |
| MS.gene58034 | MS.gene08324 | PPI |
| MS.gene08324 | MS.gene65381 | PPI |
| MS.gene24461 | MS.gene08324 | PPI |
| MS.gene58033 | MS.gene08324 | PPI |
| MS.gene75800 | MS.gene08324 | PPI |
| MS.gene074497 | MS.gene08324 | PPI |
| MS.gene66443 | MS.gene08324 | PPI |
| MS.gene08324 | MS.gene036713 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08324.t1 | MTR_4g127440 | 54.610 | 564 | 223 | 9 | 1 | 537 | 1 | 558 | 0.0 | 564 |
| MS.gene08324.t1 | MTR_2g037980 | 75.000 | 316 | 79 | 0 | 1 | 316 | 1 | 316 | 4.78e-178 | 515 |
| MS.gene08324.t1 | MTR_4g115420 | 49.912 | 567 | 222 | 10 | 2 | 535 | 4 | 541 | 1.32e-171 | 497 |
| MS.gene08324.t1 | MTR_0530s0020 | 39.194 | 546 | 256 | 16 | 21 | 535 | 6 | 506 | 3.83e-110 | 339 |
| MS.gene08324.t1 | MTR_4g114960 | 31.317 | 562 | 299 | 16 | 8 | 506 | 7 | 544 | 3.80e-77 | 255 |
| MS.gene08324.t1 | MTR_8g008860 | 32.593 | 540 | 311 | 15 | 8 | 504 | 7 | 536 | 5.99e-68 | 230 |
| MS.gene08324.t1 | MTR_2g008170 | 38.393 | 336 | 167 | 6 | 6 | 309 | 5 | 332 | 2.82e-64 | 221 |
| MS.gene08324.t1 | MTR_3g080490 | 34.783 | 322 | 194 | 6 | 8 | 322 | 6 | 318 | 2.20e-61 | 218 |
| MS.gene08324.t1 | MTR_3g106900 | 48.052 | 154 | 76 | 3 | 3 | 154 | 2 | 153 | 6.86e-37 | 145 |
| MS.gene08324.t1 | MTR_3g106900 | 34.877 | 367 | 185 | 11 | 181 | 506 | 227 | 580 | 2.55e-27 | 117 |
| MS.gene08324.t1 | MTR_8g106040 | 28.803 | 309 | 205 | 8 | 7 | 307 | 5 | 306 | 1.19e-35 | 139 |
| MS.gene08324.t1 | MTR_6g059380 | 25.000 | 364 | 220 | 12 | 8 | 344 | 10 | 347 | 1.80e-23 | 104 |
| MS.gene08324.t1 | MTR_1g033880 | 25.076 | 331 | 206 | 12 | 1 | 319 | 1 | 301 | 2.81e-15 | 78.2 |
| MS.gene08324.t1 | MTR_3g086670 | 33.548 | 155 | 96 | 4 | 8 | 159 | 6 | 156 | 7.15e-15 | 76.3 |
| MS.gene08324.t1 | MTR_8g089850 | 30.178 | 169 | 106 | 5 | 34 | 199 | 31 | 190 | 3.80e-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08324.t1 | AT2G01600 | 70.719 | 584 | 137 | 9 | 1 | 563 | 1 | 571 | 0.0 | 786 |
| MS.gene08324.t1 | AT1G14910 | 69.381 | 565 | 153 | 8 | 1 | 559 | 1 | 551 | 0.0 | 769 |
| MS.gene08324.t1 | AT1G14910 | 68.496 | 565 | 153 | 9 | 1 | 559 | 1 | 546 | 0.0 | 750 |
| MS.gene08324.t1 | AT4G25940 | 57.383 | 535 | 184 | 9 | 1 | 498 | 1 | 528 | 0.0 | 566 |
| MS.gene08324.t1 | AT5G57200 | 76.266 | 316 | 74 | 1 | 1 | 316 | 1 | 315 | 3.45e-179 | 518 |
| MS.gene08324.t1 | AT5G35200 | 51.351 | 518 | 202 | 11 | 8 | 511 | 12 | 493 | 1.77e-164 | 479 |
| MS.gene08324.t1 | AT5G35200 | 51.351 | 518 | 202 | 11 | 8 | 511 | 12 | 493 | 1.77e-164 | 479 |
| MS.gene08324.t1 | AT1G03050 | 34.483 | 580 | 286 | 21 | 1 | 506 | 1 | 560 | 2.42e-77 | 256 |
| MS.gene08324.t1 | AT4G32285 | 32.203 | 590 | 295 | 15 | 6 | 506 | 4 | 577 | 1.16e-73 | 247 |
| MS.gene08324.t1 | AT4G32285 | 32.203 | 590 | 295 | 15 | 6 | 506 | 4 | 577 | 1.16e-73 | 247 |
| MS.gene08324.t1 | AT4G02650 | 32.000 | 575 | 296 | 19 | 8 | 506 | 7 | 562 | 6.64e-71 | 239 |
| MS.gene08324.t1 | AT1G05020 | 34.969 | 326 | 185 | 6 | 8 | 309 | 6 | 328 | 4.43e-58 | 205 |
| MS.gene08324.t1 | AT2G25430 | 51.007 | 149 | 69 | 3 | 6 | 152 | 4 | 150 | 7.17e-39 | 151 |
| MS.gene08324.t1 | AT2G25430 | 30.914 | 372 | 189 | 10 | 181 | 506 | 247 | 596 | 2.07e-28 | 120 |
| MS.gene08324.t1 | AT1G33340 | 27.687 | 307 | 200 | 7 | 7 | 305 | 9 | 301 | 5.61e-29 | 119 |
| MS.gene08324.t1 | AT4G40080 | 32.367 | 207 | 127 | 6 | 1 | 199 | 1 | 202 | 1.68e-21 | 97.1 |
| MS.gene08324.t1 | AT1G25240 | 29.012 | 324 | 183 | 11 | 4 | 315 | 2 | 290 | 2.73e-21 | 96.3 |
| MS.gene08324.t1 | AT1G25240 | 29.012 | 324 | 183 | 11 | 4 | 315 | 1 | 289 | 2.80e-21 | 96.3 |
| MS.gene08324.t1 | AT1G68110 | 25.079 | 315 | 202 | 10 | 4 | 307 | 1 | 292 | 4.83e-18 | 86.7 |
| MS.gene08324.t1 | AT1G14686 | 24.684 | 316 | 194 | 12 | 4 | 310 | 1 | 281 | 3.08e-11 | 65.5 |
Find 160 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTTTGCGGATTCAGGATT+TGG | 0.185619 | 4.1:+16728502 | MS.gene08324:CDS |
| GCAAATCCCTTTGCGGATTC+AGG | 0.201574 | 4.1:+16728496 | MS.gene08324:CDS |
| ACTTCTTAGATGTCTTTCTT+TGG | 0.204881 | 4.1:-16721673 | None:intergenic |
| GCTGCACAACAACCAGTTTA+TGG | 0.213469 | 4.1:+16728298 | MS.gene08324:CDS |
| CTGCTGACCCGCACGCTTAT+AGG | 0.229577 | 4.1:-16726821 | None:intergenic |
| TCCTGTCCTTGGGCAGGTTT+GGG | 0.249846 | 4.1:-16723642 | None:intergenic |
| CTCCTGTCCTTGGGCAGGTT+TGG | 0.252819 | 4.1:-16723643 | None:intergenic |
| CCTTTGCGGATTCAGGATTT+GGG | 0.252980 | 4.1:+16728503 | MS.gene08324:CDS |
| TCATGAACTTCAAATGGATT+TGG | 0.265369 | 4.1:-16728331 | None:intergenic |
| CTTCTTAGATGTCTTTCTTT+GGG | 0.274846 | 4.1:-16721672 | None:intergenic |
| GCCATAGTCTCCACTGAAAA+TGG | 0.279408 | 4.1:+16727597 | MS.gene08324:CDS |
| CATGAACTTCAAATGGATTT+GGG | 0.280536 | 4.1:-16728330 | None:intergenic |
| TTCTGATGGCACCTGTTCTT+CGG | 0.286225 | 4.1:-16727347 | None:intergenic |
| GACTTGCTAAGACGCGAAAT+TGG | 0.292068 | 4.1:+16721897 | MS.gene08324:CDS |
| TTTAGATAGTGAGGAGCTAT+TGG | 0.306314 | 4.1:+16724667 | MS.gene08324:CDS |
| CAACTATGGAAGAGTACATT+AGG | 0.314951 | 4.1:+16727137 | MS.gene08324:CDS |
| CAGTCTTGGCGAAGAGTTTA+CGG | 0.318642 | 4.1:+16721455 | MS.gene08324:CDS |
| TTGCTAACAGCTGCTCCTTC+TGG | 0.320696 | 4.1:-16724879 | None:intergenic |
| TCAATTGCAAGAACTTCTTC+TGG | 0.323093 | 4.1:-16727310 | None:intergenic |
| GGAGGTGCAGGCTCAGAATT+GGG | 0.326530 | 4.1:-16727394 | None:intergenic |
| AAGAGCATTTCTTTCCTCTA+TGG | 0.329697 | 4.1:-16727568 | None:intergenic |
| TCTGATGGCACCTGTTCTTC+GGG | 0.330942 | 4.1:-16727346 | None:intergenic |
| TGCCATTTGGACAGCGGCCG+GGG | 0.331631 | 4.1:-16728383 | None:intergenic |
| AAGAAAGACTTGAATGCTTT+CGG | 0.338922 | 4.1:+16723586 | MS.gene08324:CDS |
| GAATTACCTTCTCCTGTCCT+TGG | 0.343652 | 4.1:-16723653 | None:intergenic |
| CAACTTCTGCTGTTCGTCCC+AGG | 0.346336 | 4.1:+16721837 | MS.gene08324:CDS |
| GCTGTTGCATTCCTGCCATT+TGG | 0.350069 | 4.1:-16728396 | None:intergenic |
| TCCTCTATGGATGATGCATT+AGG | 0.359288 | 4.1:-16727555 | None:intergenic |
| GCAAGTCTCCAGTGTCAAAA+TGG | 0.362620 | 4.1:-16727438 | None:intergenic |
| CAGCCTGGGATTGTTCTGCC+TGG | 0.365265 | 4.1:+16723538 | MS.gene08324:intron |
| TTATTATGTGGGTGAGAAAC+GGG | 0.370777 | 4.1:-16728541 | None:intergenic |
| AGGAGGTGCAGGCTCAGAAT+TGG | 0.371133 | 4.1:-16727395 | None:intergenic |
| GCTGCTGATGAGGCTGAGGC+TGG | 0.373860 | 4.1:-16728450 | None:intergenic |
| AACTTTGGTGGTATCTTTGA+CGG | 0.380674 | 4.1:-16721480 | None:intergenic |
| GGGGCTGGTGCTCCATAAAC+TGG | 0.381353 | 4.1:-16728310 | None:intergenic |
| CATCCTTAAAATTTGAAAGT+TGG | 0.385074 | 4.1:-16723359 | None:intergenic |
| CAGTACTCGGAGTGCTGACC+AGG | 0.388662 | 4.1:-16727789 | None:intergenic |
| TGGATGATGCATTAGGTTCC+AGG | 0.391284 | 4.1:-16727548 | None:intergenic |
| GGTGGTGGAGGAGGAGGTGC+AGG | 0.394378 | 4.1:-16727406 | None:intergenic |
| TGAGATGGTGGTGGAGGAGG+AGG | 0.402344 | 4.1:-16727412 | None:intergenic |
| CCACATAATAACAATCCATT+TGG | 0.402689 | 4.1:+16728553 | MS.gene08324:CDS |
| CCTGCCATTTGGACAGCGGC+CGG | 0.404720 | 4.1:-16728385 | None:intergenic |
| TGTCAAAGAGCCCGAAGAAC+AGG | 0.409894 | 4.1:+16727336 | MS.gene08324:CDS |
| CTATGAAGTTTGCAAAGGAT+TGG | 0.410819 | 4.1:+16726957 | MS.gene08324:CDS |
| AATGGATCATGAACTTCAAA+TGG | 0.420594 | 4.1:-16728337 | None:intergenic |
| TATATCGACTTGTTGGCTGC+CGG | 0.425212 | 4.1:+16724717 | MS.gene08324:CDS |
| AATTACCTTCTCCTGTCCTT+GGG | 0.427574 | 4.1:-16723652 | None:intergenic |
| GCAGCAGCAGCATATGTTGA+TGG | 0.428102 | 4.1:+16728468 | MS.gene08324:CDS |
| CCGCACGCTTATAGGCTTCA+AGG | 0.429737 | 4.1:-16726813 | None:intergenic |
| GAATCCGCAAAGGGATTTGC+AGG | 0.431243 | 4.1:-16728493 | None:intergenic |
| ACCTTCTCCTGTCCTTGGGC+AGG | 0.431535 | 4.1:-16723648 | None:intergenic |
| TTGTGAGATGGTGGTGGAGG+AGG | 0.437159 | 4.1:-16727415 | None:intergenic |
| TACTGGATGGGAGCTTGCCC+TGG | 0.437527 | 4.1:+16727771 | MS.gene08324:CDS |
| TGGACATTCAACGTGATTAG+TGG | 0.438730 | 4.1:-16721649 | None:intergenic |
| GGAATGTCAGGCTGGTGGAC+TGG | 0.439998 | 4.1:+16728231 | MS.gene08324:intron |
| TGCTGCTGCTGATGAGGCTG+AGG | 0.440595 | 4.1:-16728454 | None:intergenic |
| CTGCCATTTGGACAGCGGCC+GGG | 0.440665 | 4.1:-16728384 | None:intergenic |
| CAGCTTCTATATCGACTTGT+TGG | 0.441624 | 4.1:+16724710 | MS.gene08324:CDS |
| AGCCTGGGATTGTTCTGCCT+GGG | 0.446845 | 4.1:+16723539 | MS.gene08324:intron |
| CAACAGCAAGCGAATCCATT+TGG | 0.449659 | 4.1:+16728412 | MS.gene08324:CDS |
| GCTTTAATAGCTTCATGTCT+TGG | 0.453459 | 4.1:-16726790 | None:intergenic |
| ACAAAAGATTTCGATCCTAC+TGG | 0.455678 | 4.1:+16727754 | MS.gene08324:CDS |
| AACAAGCCGGTGCTTCCAAA+TGG | 0.457190 | 4.1:-16728568 | None:intergenic |
| AACTTCTGCTGTTCGTCCCA+GGG | 0.458247 | 4.1:+16721838 | MS.gene08324:CDS |
| ATTTACTGCGCTATTAATGA+TGG | 0.459826 | 4.1:+16726650 | MS.gene08324:CDS |
| GTACTAACCATCGGCTCACT+TGG | 0.460104 | 4.1:-16727184 | None:intergenic |
| CACCCTCCCTGAGTAACCTA+TGG | 0.461427 | 4.1:-16723284 | None:intergenic |
| CCAAATGGATTGTTATTATG+TGG | 0.463425 | 4.1:-16728553 | None:intergenic |
| GTTATTATGTGGGTGAGAAA+CGG | 0.463914 | 4.1:-16728542 | None:intergenic |
| AACAATCCATTTGGAAGCAC+CGG | 0.464105 | 4.1:+16728562 | MS.gene08324:CDS |
| TTCAGTTAATGAGAGACAAT+TGG | 0.465026 | 4.1:+16727819 | MS.gene08324:CDS |
| AACTGTGGGTAAGGACCAAA+TGG | 0.466710 | 4.1:-16728427 | None:intergenic |
| ATATCGACTTGTTGGCTGCC+GGG | 0.467584 | 4.1:+16724718 | MS.gene08324:CDS |
| GCAAAGGATTGGAACTTGCT+AGG | 0.473319 | 4.1:+16726968 | MS.gene08324:CDS |
| CTATAAGCGTGCGGGTCAGC+AGG | 0.476852 | 4.1:+16726822 | MS.gene08324:CDS |
| GTACATTAGGGAGGCACCCC+GGG | 0.477247 | 4.1:+16727150 | MS.gene08324:CDS |
| GACATGGGCGAGACCAACTT+TGG | 0.477335 | 4.1:-16721495 | None:intergenic |
| ATGTTGCTTGATATAGCAAA+TGG | 0.483557 | 4.1:-16728355 | None:intergenic |
| ATCAAGCAACATTGCTCCCC+CGG | 0.485362 | 4.1:+16728366 | MS.gene08324:CDS |
| CTTTGCGGATTCAGGATTTG+GGG | 0.488384 | 4.1:+16728504 | MS.gene08324:CDS |
| CACTTGGAACATTAACAACC+CGG | 0.489997 | 4.1:-16727168 | None:intergenic |
| AAGGATGACTCTAGTCCAAT+CGG | 0.491603 | 4.1:+16723375 | MS.gene08324:CDS |
| CTTAACAGCTTATATGATGA+AGG | 0.493587 | 4.1:+16728265 | MS.gene08324:CDS |
| TGAGGCTGGAACTGTGGGTA+AGG | 0.498922 | 4.1:-16728436 | None:intergenic |
| AACTATGGAAGAGTACATTA+GGG | 0.503486 | 4.1:+16727138 | MS.gene08324:CDS |
| CTTAGATGTCTTTCTTTGGG+TGG | 0.506041 | 4.1:-16721669 | None:intergenic |
| GGACCCTGCAAATCCCTTTG+CGG | 0.509785 | 4.1:+16728489 | MS.gene08324:CDS |
| TATAGAAGCTGTTGTAAAGC+AGG | 0.509890 | 4.1:-16724698 | None:intergenic |
| AGAATTTCCTCTCTAAGCGT+TGG | 0.511778 | 4.1:-16723309 | None:intergenic |
| TAGTCATCCATAGGTTACTC+AGG | 0.518273 | 4.1:+16723277 | MS.gene08324:CDS |
| CCGCGTAATCGCTATTGACA+TGG | 0.523311 | 4.1:-16721511 | None:intergenic |
| CGCACGCTTATAGGCTTCAA+GGG | 0.523909 | 4.1:-16726812 | None:intergenic |
| ATATGCTGCTGCTGCTGATG+AGG | 0.524715 | 4.1:-16728460 | None:intergenic |
| ACCCAAACCTGCCCAAGGAC+AGG | 0.527781 | 4.1:+16723641 | MS.gene08324:CDS |
| CATTATCAATCTTGTTGACA+AGG | 0.528067 | 4.1:+16726673 | MS.gene08324:CDS |
| ACAATAAGCAACATCAGCCC+TGG | 0.528722 | 4.1:-16721855 | None:intergenic |
| CCGGCCGCTGTCCAAATGGC+AGG | 0.532125 | 4.1:+16728385 | MS.gene08324:CDS |
| TGGTTGTGAGATGGTGGTGG+AGG | 0.535548 | 4.1:-16727418 | None:intergenic |
| TGCTAAGACGCGAAATTGGA+CGG | 0.536350 | 4.1:+16721901 | MS.gene08324:CDS |
| CAATGGGTACTCTTCAGTCT+TGG | 0.538882 | 4.1:+16721441 | None:intergenic |
| CCAAATCCTGAATCCGCAAA+GGG | 0.540155 | 4.1:-16728502 | None:intergenic |
| AGCAGGGACACAGCAGGACT+AGG | 0.541151 | 4.1:+16724642 | MS.gene08324:intron |
| ACTTCAAATGGATTTGGGGC+TGG | 0.546087 | 4.1:-16728325 | None:intergenic |
| CATTCCTGCCATTTGGACAG+CGG | 0.548232 | 4.1:-16728389 | None:intergenic |
| GCCATTTGGACAGCGGCCGG+GGG | 0.549447 | 4.1:-16728382 | None:intergenic |
| GATACAATATGCGCTAGCTC+TGG | 0.549765 | 4.1:+16724908 | MS.gene08324:CDS |
| TTATTATCACTAGGTTCTGA+TGG | 0.551694 | 4.1:-16727361 | None:intergenic |
| TCTTATTTCTTACAACAAGC+CGG | 0.553829 | 4.1:-16728581 | None:intergenic |
| AAGATTTCGATCCTACTGGA+TGG | 0.558420 | 4.1:+16727758 | MS.gene08324:CDS |
| GCAGGGACACAGCAGGACTA+GGG | 0.563205 | 4.1:+16724643 | MS.gene08324:intron |
| GTCAAAGATACCACCAAAGT+TGG | 0.563537 | 4.1:+16721482 | MS.gene08324:CDS |
| ACCTAATGCATCATCCATAG+AGG | 0.564544 | 4.1:+16727554 | MS.gene08324:CDS |
| AAATGGTTGTGAGATGGTGG+TGG | 0.566222 | 4.1:-16727421 | None:intergenic |
| TGTGAATATAGGCACTGCAT+CGG | 0.567918 | 4.1:+16727708 | MS.gene08324:intron |
| CAAATGGATTGTTATTATGT+GGG | 0.570554 | 4.1:-16728552 | None:intergenic |
| AGACATACCTCTCTTAAGAC+AGG | 0.571173 | 4.1:-16727003 | None:intergenic |
| TAATGTTCCAAGTGAGCCGA+TGG | 0.572868 | 4.1:+16727177 | MS.gene08324:CDS |
| TGAGGCTGAGGCTGGAACTG+TGG | 0.573458 | 4.1:-16728442 | None:intergenic |
| GAGGTGCAGGCTCAGAATTG+GGG | 0.576097 | 4.1:-16727393 | None:intergenic |
| GATTTCTATGAAGTTTGCAA+AGG | 0.576897 | 4.1:+16726952 | MS.gene08324:CDS |
| AGGTGCAGGCTCAGAATTGG+GGG | 0.577902 | 4.1:-16727392 | None:intergenic |
| GGAAAGAAATGCTCTTGCGC+TGG | 0.578170 | 4.1:+16727575 | MS.gene08324:CDS |
| TCAGTTTCCTGTCTTAAGAG+AGG | 0.581783 | 4.1:+16726996 | MS.gene08324:CDS |
| ACCTGCCCAAGGACAGGAGA+AGG | 0.586183 | 4.1:+16723647 | MS.gene08324:CDS |
| CCCAAATCCTGAATCCGCAA+AGG | 0.589248 | 4.1:-16728503 | None:intergenic |
| ATGGGCGAGACCAACTTTGG+TGG | 0.590062 | 4.1:-16721492 | None:intergenic |
| AAACACTGATAGTCATCCAT+AGG | 0.590218 | 4.1:+16723268 | MS.gene08324:CDS |
| GTCATGGAATGTCAGGCTGG+TGG | 0.590289 | 4.1:+16728226 | MS.gene08324:intron |
| AGTACATTAGGGAGGCACCC+CGG | 0.591792 | 4.1:+16727149 | MS.gene08324:CDS |
| TACCCAGGCAGAACAATCCC+AGG | 0.597737 | 4.1:-16723541 | None:intergenic |
| GACTAGGGATTTAGATAGTG+AGG | 0.598908 | 4.1:+16724658 | MS.gene08324:CDS |
| ACTTGGAACATTAACAACCC+GGG | 0.599939 | 4.1:-16727167 | None:intergenic |
| ATCCATAGGTTACTCAGGGA+GGG | 0.600254 | 4.1:+16723282 | MS.gene08324:CDS |
| CTTGAAGCCTATAAGCGTGC+GGG | 0.602046 | 4.1:+16726814 | MS.gene08324:CDS |
| AGATTTCGATCCTACTGGAT+GGG | 0.602139 | 4.1:+16727759 | MS.gene08324:CDS |
| ATGAACTTCAAATGGATTTG+GGG | 0.604886 | 4.1:-16728329 | None:intergenic |
| GAGGCTGAGGCTGGAACTGT+GGG | 0.606106 | 4.1:-16728441 | None:intergenic |
| CAATAAGCAACATCAGCCCT+GGG | 0.614387 | 4.1:-16721854 | None:intergenic |
| TCAAAATGGTTGTGAGATGG+TGG | 0.614778 | 4.1:-16727424 | None:intergenic |
| TTTGCGGATTCAGGATTTGG+GGG | 0.625862 | 4.1:+16728505 | MS.gene08324:CDS |
| ACTGAAGAAATATCAGTACT+CGG | 0.630374 | 4.1:-16727802 | None:intergenic |
| CGCGTAATCGCTATTGACAT+GGG | 0.631037 | 4.1:-16721510 | None:intergenic |
| AGTCATCCATAGGTTACTCA+GGG | 0.631167 | 4.1:+16723278 | MS.gene08324:CDS |
| TATGTAGGGATTGAATGACC+TGG | 0.635485 | 4.1:+16727530 | MS.gene08324:intron |
| GGGTGATCCAACGCTTAGAG+AGG | 0.638082 | 4.1:+16723302 | MS.gene08324:CDS |
| CCTTGAAGCCTATAAGCGTG+CGG | 0.638815 | 4.1:+16726813 | MS.gene08324:CDS |
| CGTCTACCCAAACCTGCCCA+AGG | 0.639108 | 4.1:+16723636 | MS.gene08324:CDS |
| CATCCATAGGTTACTCAGGG+AGG | 0.645447 | 4.1:+16723281 | MS.gene08324:CDS |
| AGGGCAAGCTCCCATCCAGT+AGG | 0.647054 | 4.1:-16727769 | None:intergenic |
| TAATGCATAGGTACGTACCC+AGG | 0.647422 | 4.1:-16723556 | None:intergenic |
| CTATAAATTACACAGCCAGA+AGG | 0.652759 | 4.1:+16724864 | MS.gene08324:intron |
| CTTGGAACATTAACAACCCG+GGG | 0.656680 | 4.1:-16727166 | None:intergenic |
| GTGTCAAAATGGTTGTGAGA+TGG | 0.662795 | 4.1:-16727427 | None:intergenic |
| AATCCGCAAAGGGATTTGCA+GGG | 0.665370 | 4.1:-16728492 | None:intergenic |
| GTTGTAAGAAAAGATTGCGG+AGG | 0.668024 | 4.1:-16727118 | None:intergenic |
| GCAGCAACATTATTATCACT+AGG | 0.687838 | 4.1:-16727370 | None:intergenic |
| TATGGAAGAGTACATTAGGG+AGG | 0.705602 | 4.1:+16727141 | MS.gene08324:CDS |
| AGTACTCGGAGTGCTGACCA+GGG | 0.708772 | 4.1:-16727788 | None:intergenic |
| CCATGTCAATAGCGATTACG+CGG | 0.714918 | 4.1:+16721511 | MS.gene08324:CDS |
| ATAGTTGTAAGAAAAGATTG+CGG | 0.742982 | 4.1:-16727121 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 16721443 | 16728594 | 16721443 | ID=MS.gene08324 |
| chr4.1 | mRNA | 16721443 | 16728594 | 16721443 | ID=MS.gene08324.t1;Parent=MS.gene08324 |
| chr4.1 | exon | 16721443 | 16721532 | 16721443 | ID=MS.gene08324.t1.exon1;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16721443 | 16721532 | 16721443 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16721624 | 16721693 | 16721624 | ID=MS.gene08324.t1.exon2;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16721624 | 16721693 | 16721624 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16721825 | 16721922 | 16721825 | ID=MS.gene08324.t1.exon3;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16721825 | 16721922 | 16721825 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16723258 | 16723396 | 16723258 | ID=MS.gene08324.t1.exon4;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16723258 | 16723396 | 16723258 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16723541 | 16723668 | 16723541 | ID=MS.gene08324.t1.exon5;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16723541 | 16723668 | 16723541 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16724647 | 16724739 | 16724647 | ID=MS.gene08324.t1.exon6;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16724647 | 16724739 | 16724647 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16724879 | 16724929 | 16724879 | ID=MS.gene08324.t1.exon7;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16724879 | 16724929 | 16724879 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16726629 | 16726694 | 16726629 | ID=MS.gene08324.t1.exon8;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16726629 | 16726694 | 16726629 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16726778 | 16726843 | 16726778 | ID=MS.gene08324.t1.exon9;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16726778 | 16726843 | 16726778 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16726937 | 16727017 | 16726937 | ID=MS.gene08324.t1.exon10;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16726937 | 16727017 | 16726937 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16727118 | 16727198 | 16727118 | ID=MS.gene08324.t1.exon11;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16727118 | 16727198 | 16727118 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16727292 | 16727462 | 16727292 | ID=MS.gene08324.t1.exon12;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16727292 | 16727462 | 16727292 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16727537 | 16727618 | 16727537 | ID=MS.gene08324.t1.exon13;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16727537 | 16727618 | 16727537 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16727719 | 16727840 | 16727719 | ID=MS.gene08324.t1.exon14;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16727719 | 16727840 | 16727719 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| chr4.1 | exon | 16728241 | 16728594 | 16728241 | ID=MS.gene08324.t1.exon15;Parent=MS.gene08324.t1 |
| chr4.1 | CDS | 16728241 | 16728594 | 16728241 | ID=cds.MS.gene08324.t1;Parent=MS.gene08324.t1 |
| Gene Sequence |
| Protein sequence |
