AlfalfaGEDB | MS.gene061578

M. sativa cultivar XinJiangDaYe / MS.gene061578

KEGG: -
GO: GO:0003674 GO:0003824
PFAM: PF00501.31 PF13193.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061578.t1	XP_003600627.1	91.8	582	10	2	1	544	1	582	1.60E-306	1061.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061578.t1	Q9SS01	57.7	582	190	7	1	533	1	575	2.0e-180	633.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061578.t1	G7J7Y4	91.8	582	10	2	1	544	1	582	1.1e-306	1061.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04933	MS.gene061578	0.819858	9.31E-53	-1.69E-46
MS.gene049351	MS.gene061578	0.805999	1.02E-49	-1.69E-46
MS.gene049681	MS.gene061578	0.990909	6.30E-185	-1.69E-46
MS.gene05028	MS.gene061578	0.886926	2.23E-72	-1.69E-46
MS.gene050397	MS.gene061578	0.802697	4.96E-49	-1.69E-46
MS.gene051255	MS.gene061578	0.811412	7.09E-51	-1.69E-46
MS.gene053836	MS.gene061578	0.821998	3.00E-53	-1.69E-46
MS.gene054010	MS.gene061578	0.812976	3.23E-51	-1.69E-46
MS.gene054011	MS.gene061578	0.833726	4.52E-56	-1.69E-46
MS.gene054012	MS.gene061578	0.82522	5.28E-54	-1.69E-46
MS.gene054013	MS.gene061578	0.822649	2.12E-53	-1.69E-46
MS.gene054173	MS.gene061578	0.805602	1.23E-49	-1.69E-46
MS.gene055277	MS.gene061578	0.82307	1.69E-53	-1.69E-46
MS.gene055422	MS.gene061578	0.80259	5.22E-49	-1.69E-46
MS.gene055428	MS.gene061578	0.80226	6.10E-49	-1.69E-46
MS.gene055604	MS.gene061578	0.835277	1.84E-56	-1.69E-46
MS.gene056191	MS.gene061578	0.842788	2.09E-58	-1.69E-46
MS.gene05702	MS.gene061578	0.820792	5.69E-53	-1.69E-46
MS.gene057255	MS.gene061578	0.805151	1.53E-49	-1.69E-46
MS.gene057749	MS.gene061578	0.812965	3.25E-51	-1.69E-46
MS.gene057823	MS.gene061578	0.814506	1.49E-51	-1.69E-46
MS.gene058002	MS.gene061578	0.801619	8.27E-49	-1.69E-46
MS.gene05811	MS.gene061578	0.809086	2.25E-50	-1.69E-46
MS.gene05907	MS.gene061578	0.807905	4.02E-50	-1.69E-46
MS.gene059294	MS.gene061578	0.800042	1.73E-48	-1.69E-46
MS.gene059974	MS.gene061578	0.802839	4.64E-49	-1.69E-46
MS.gene060005	MS.gene061578	0.809579	1.76E-50	-1.69E-46
MS.gene06037	MS.gene061578	0.820702	5.97E-53	-1.69E-46
MS.gene060559	MS.gene061578	0.802571	5.27E-49	-1.69E-46
MS.gene061049	MS.gene061578	0.813171	2.93E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene09098	MS.gene061578	PPI
MS.gene99857	MS.gene061578	PPI
MS.gene070510	MS.gene061578	PPI
MS.gene81622	MS.gene061578	PPI
MS.gene31229	MS.gene061578	PPI
MS.gene016743	MS.gene061578	PPI
MS.gene061578	MS.gene024712	PPI
MS.gene024710	MS.gene061578	PPI
MS.gene37911	MS.gene061578	PPI
MS.gene057884	MS.gene061578	PPI
MS.gene049797	MS.gene061578	PPI
MS.gene061578	MS.gene91712	PPI
MS.gene025344	MS.gene061578	PPI
MS.gene88460	MS.gene061578	PPI
MS.gene061578	MS.gene016573	PPI
MS.gene061578	MS.gene070511	PPI
MS.gene057883	MS.gene061578	PPI
MS.gene90308	MS.gene061578	PPI
MS.gene061578	MS.gene049681	PPI
MS.gene36348	MS.gene061578	PPI
MS.gene049798	MS.gene061578	PPI
MS.gene016758	MS.gene061578	PPI
MS.gene061175	MS.gene061578	PPI
MS.gene007715	MS.gene061578	PPI
MS.gene27218	MS.gene061578	PPI
MS.gene43857	MS.gene061578	PPI
MS.gene049799	MS.gene061578	PPI
MS.gene09104	MS.gene061578	PPI
MS.gene99100	MS.gene061578	PPI
MS.gene061578	MS.gene67840	PPI
MS.gene85146	MS.gene061578	PPI
MS.gene81617	MS.gene061578	PPI
MS.gene061578	MS.gene26216	PPI
MS.gene061578	MS.gene27109	PPI
MS.gene83513	MS.gene061578	PPI
MS.gene061578	MS.gene88935	PPI
MS.gene008350	MS.gene061578	PPI
MS.gene070505	MS.gene061578	PPI
MS.gene017552	MS.gene061578	PPI
MS.gene061578	MS.gene88987	PPI
MS.gene057886	MS.gene061578	PPI
MS.gene070509	MS.gene061578	PPI
MS.gene061578	MS.gene27213	PPI
MS.gene061578	MS.gene09153	PPI
MS.gene070503	MS.gene061578	PPI
MS.gene061578	MS.gene061174	PPI
MS.gene23788	MS.gene061578	PPI
MS.gene041843	MS.gene061578	PPI
MS.gene061578	MS.gene21691	PPI
MS.gene061578	MS.gene037813	PPI
MS.gene23786	MS.gene061578	PPI
MS.gene041844	MS.gene061578	PPI
MS.gene037817	MS.gene061578	PPI
MS.gene070507	MS.gene061578	PPI
MS.gene061578	MS.gene017279	PPI
MS.gene061578	MS.gene24088	PPI
MS.gene81620	MS.gene061578	PPI
MS.gene21937	MS.gene061578	PPI
MS.gene061578	MS.gene09948	PPI
MS.gene025787	MS.gene061578	PPI
MS.gene061578	MS.gene98148	PPI
MS.gene22277	MS.gene061578	PPI
MS.gene21145	MS.gene061578	PPI
MS.gene64546	MS.gene061578	PPI
MS.gene81615	MS.gene061578	PPI
MS.gene028660	MS.gene061578	PPI
MS.gene017547	MS.gene061578	PPI
MS.gene23782	MS.gene061578	PPI
MS.gene017545	MS.gene061578	PPI
MS.gene85145	MS.gene061578	PPI
MS.gene037815	MS.gene061578	PPI
MS.gene34290	MS.gene061578	PPI
MS.gene061578	MS.gene61755	PPI
MS.gene069865	MS.gene061578	PPI
MS.gene21689	MS.gene061578	PPI
MS.gene05028	MS.gene061578	PPI
MS.gene61077	MS.gene061578	PPI
MS.gene061578	MS.gene76530	PPI
MS.gene061578	MS.gene034275	PPI
MS.gene009614	MS.gene061578	PPI
MS.gene09107	MS.gene061578	PPI
MS.gene061578	MS.gene015458	PPI
MS.gene36347	MS.gene061578	PPI
MS.gene070492	MS.gene061578	PPI
MS.gene016742	MS.gene061578	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061578.t1	MTR_3g064420	91.753	582	10	2	1	544	1	582	0.0	1094
MS.gene061578.t1	MTR_8g074040	48.108	555	235	7	1	516	43	583	0.0	549
MS.gene061578.t1	MTR_5g008800	48.101	553	236	5	1	516	1	539	0.0	544
MS.gene061578.t1	MTR_5g076240	50.534	562	213	8	1	514	1	545	0.0	540
MS.gene061578.t1	MTR_4g121600	48.294	557	238	8	1	514	7	556	4.11e-180	519
MS.gene061578.t1	MTR_2g044180	47.756	557	241	7	1	514	7	556	1.12e-178	515
MS.gene061578.t1	MTR_5g008820	45.355	549	251	5	1	514	1	535	2.79e-175	506
MS.gene061578.t1	MTR_5g076220	48.741	556	233	7	1	514	1	546	3.28e-175	506
MS.gene061578.t1	MTR_5g076200	47.482	556	240	8	1	514	1	546	7.25e-171	495
MS.gene061578.t1	MTR_0161s0050	46.263	562	240	7	1	514	1	548	1.23e-164	479
MS.gene061578.t1	MTR_8g074040	48.434	479	194	7	77	516	1	465	5.84e-164	475
MS.gene061578.t1	MTR_8g074120	47.035	489	206	7	67	516	20	494	2.92e-159	464
MS.gene061578.t1	MTR_2g044180	46.610	472	202	7	86	514	1	465	5.58e-142	418
MS.gene061578.t1	MTR_5g023730	38.087	554	285	11	3	514	4	541	2.93e-122	370
MS.gene061578.t1	MTR_0803s0020	29.851	536	306	11	14	509	235	740	1.75e-55	202
MS.gene061578.t1	MTR_2g105570	28.200	539	305	17	25	512	37	544	1.64e-42	160
MS.gene061578.t1	MTR_5g007640	27.574	544	311	14	18	512	73	582	4.26e-40	154
MS.gene061578.t1	MTR_4g057730	25.730	548	325	14	14	514	27	539	6.15e-39	150
MS.gene061578.t1	MTR_2g095510	28.159	554	303	19	14	515	27	537	1.09e-37	146
MS.gene061578.t1	MTR_3g460770	25.641	546	312	16	19	512	33	536	7.55e-36	141
MS.gene061578.t1	MTR_1569s0010	32.249	369	176	12	192	511	186	529	2.15e-34	136
MS.gene061578.t1	MTR_3g035130	28.366	557	296	18	15	517	5	512	2.62e-34	136
MS.gene061578.t1	MTR_1g063110	26.642	548	315	18	14	509	28	540	6.72e-34	135
MS.gene061578.t1	MTR_4g005750	27.505	549	304	18	25	519	36	544	6.84e-34	135
MS.gene061578.t1	MTR_1g026310	24.665	523	309	12	37	509	65	552	6.79e-32	129
MS.gene061578.t1	MTR_4g128337	25.636	550	313	16	18	512	35	543	9.90e-32	129
MS.gene061578.t1	MTR_8g039720	24.340	530	304	14	37	509	62	551	1.04e-31	129
MS.gene061578.t1	MTR_1g063120	25.405	555	317	16	14	509	28	544	3.62e-30	124
MS.gene061578.t1	MTR_1g090737	26.062	518	311	17	43	519	82	568	1.10e-28	120
MS.gene061578.t1	MTR_1g073180	24.277	519	299	12	39	512	60	529	1.48e-27	116
MS.gene061578.t1	MTR_1054s0020	25.996	527	313	18	27	514	281	769	1.71e-27	117
MS.gene061578.t1	MTR_4g057730	24.788	472	280	13	14	438	27	470	2.93e-27	115
MS.gene061578.t1	MTR_3g035130	27.328	494	268	14	15	455	5	460	1.94e-25	109
MS.gene061578.t1	MTR_4g011790	26.276	392	213	13	179	510	217	592	4.90e-25	109
MS.gene061578.t1	MTR_4g011790	26.209	393	214	13	179	511	217	593	7.68e-25	108
MS.gene061578.t1	MTR_2g030240	23.845	541	316	17	39	510	27	540	3.15e-24	107
MS.gene061578.t1	MTR_4g013210	26.836	354	201	13	189	510	71	398	3.65e-24	105
MS.gene061578.t1	MTR_5g027480	24.621	528	302	18	36	510	64	548	3.83e-24	106
MS.gene061578.t1	MTR_4g013210	26.410	390	198	13	189	510	71	439	8.67e-24	104
MS.gene061578.t1	MTR_4g013210	26.836	354	201	13	189	510	223	550	1.09e-23	105
MS.gene061578.t1	MTR_3g088870	27.123	365	196	10	194	509	207	550	1.29e-23	105
MS.gene061578.t1	MTR_3g088880	27.446	368	188	12	194	509	208	548	6.38e-23	102
MS.gene061578.t1	MTR_1g073180	23.566	488	279	12	39	481	82	520	4.84e-22	100
MS.gene061578.t1	MTR_4g011790	28.980	245	155	7	269	510	373	601	4.95e-22	100
MS.gene061578.t1	MTR_4g077900	34.194	155	94	2	357	510	324	471	9.70e-22	98.6
MS.gene061578.t1	MTR_5g027500	36.129	155	91	2	357	510	414	561	1.05e-21	99.4
MS.gene061578.t1	MTR_2g030240	25.954	393	221	14	180	510	62	446	1.06e-21	98.6
MS.gene061578.t1	MTR_4g057730	23.736	455	272	13	14	421	27	453	4.54e-21	96.7
MS.gene061578.t1	MTR_1586s0010	23.925	372	217	11	39	363	69	421	8.47e-21	95.5
MS.gene061578.t1	MTR_2g095510	27.171	449	239	18	14	410	27	439	5.67e-20	93.2
MS.gene061578.t1	MTR_4g013210	26.230	366	181	13	189	486	223	567	1.08e-17	86.7
MS.gene061578.t1	MTR_7g084850	33.533	167	102	3	352	513	558	720	7.73e-15	78.2
MS.gene061578.t1	MTR_7g109830	31.633	196	117	6	239	432	362	542	1.87e-13	73.6
MS.gene061578.t1	MTR_3g087980	29.520	271	130	12	269	514	422	656	4.82e-13	72.4
MS.gene061578.t1	MTR_1g030290	32.948	173	94	6	269	432	424	583	6.91e-12	68.6
MS.gene061578.t1	MTR_1g086600	31.737	167	99	6	269	432	412	566	1.88e-11	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061578.t1	AT1G65890	57.612	578	195	6	1	533	1	573	0.0	651
MS.gene061578.t1	AT1G65880	58.421	570	183	6	1	523	1	563	0.0	651
MS.gene061578.t1	AT1G66120	55.938	581	204	6	1	538	1	572	0.0	642
MS.gene061578.t1	AT1G68270	56.320	538	214	6	1	523	1	532	0.0	590
MS.gene061578.t1	AT1G20560	46.737	567	247	5	1	526	3	555	0.0	524
MS.gene061578.t1	AT3G16910	49.192	557	234	8	1	514	9	559	3.59e-177	511
MS.gene061578.t1	AT5G16340	45.863	556	245	6	1	514	1	542	2.77e-175	506
MS.gene061578.t1	AT1G21530	46.898	548	243	7	9	516	9	548	9.70e-174	502
MS.gene061578.t1	AT5G16370	45.683	556	246	6	1	514	1	542	1.73e-173	501
MS.gene061578.t1	AT2G17650	46.964	560	240	6	1	513	48	597	4.35e-173	503
MS.gene061578.t1	AT1G21530	46.886	546	244	8	9	516	9	546	2.65e-171	496
MS.gene061578.t1	AT1G21540	46.196	552	242	7	9	516	9	549	2.53e-169	491
MS.gene061578.t1	AT1G75960	45.504	556	247	5	1	514	1	542	2.50e-166	483
MS.gene061578.t1	AT1G77240	44.545	550	249	7	9	516	9	544	4.87e-165	480
MS.gene061578.t1	AT1G20560	46.029	491	210	5	77	526	1	477	1.63e-150	440
MS.gene061578.t1	AT1G76290	43.525	556	251	9	1	514	1	535	1.94e-142	422
MS.gene061578.t1	AT3G21240	27.357	541	314	13	19	512	41	549	4.84e-45	167
MS.gene061578.t1	AT3G21230	27.810	525	298	11	37	512	71	563	6.98e-41	155
MS.gene061578.t1	AT1G51680	27.839	546	310	15	19	512	43	556	6.28e-39	150
MS.gene061578.t1	AT4G05160	26.895	554	309	17	17	515	30	542	2.56e-37	145
MS.gene061578.t1	AT1G65060	27.239	547	307	19	18	512	52	559	5.80e-37	144
MS.gene061578.t1	AT1G20510	26.230	549	300	20	18	509	36	536	4.99e-36	141
MS.gene061578.t1	AT1G20480	26.718	524	295	16	37	509	72	557	9.64e-36	141
MS.gene061578.t1	AT1G20500	24.125	543	319	15	18	509	42	542	4.48e-35	139
MS.gene061578.t1	AT3G21240	26.709	468	271	12	19	439	41	483	1.88e-33	133
MS.gene061578.t1	AT3G21240	26.709	468	271	12	19	439	41	483	1.88e-33	133
MS.gene061578.t1	AT3G48990	27.255	521	277	15	40	506	31	503	6.94e-33	132
MS.gene061578.t1	AT1G51680	27.184	515	291	15	19	481	43	525	1.08e-32	132
MS.gene061578.t1	AT5G38120	26.519	543	305	17	18	509	43	542	1.59e-32	131
MS.gene061578.t1	AT4G19010	25.141	533	307	15	31	510	62	555	2.67e-32	131
MS.gene061578.t1	AT3G21230	27.419	496	275	13	37	481	71	532	3.33e-31	127
MS.gene061578.t1	AT5G63380	25.641	546	292	19	17	509	66	550	1.07e-30	126
MS.gene061578.t1	AT1G62940	25.408	551	318	15	14	512	26	535	1.75e-29	122
MS.gene061578.t1	AT1G20510	26.360	478	254	18	18	438	36	472	2.34e-28	118
MS.gene061578.t1	AT5G38120	26.408	515	285	17	18	481	43	514	5.68e-28	117
MS.gene061578.t1	AT1G30520	25.090	554	336	16	20	512	13	548	1.07e-27	117
MS.gene061578.t1	AT1G30520	25.090	554	336	16	20	512	13	548	1.07e-27	117
MS.gene061578.t1	AT1G51680	27.061	473	268	14	19	439	43	490	4.78e-27	114
MS.gene061578.t1	AT3G21230	26.549	452	258	10	37	439	71	497	1.76e-26	113
MS.gene061578.t1	AT1G65060	26.517	445	243	17	18	410	52	464	2.10e-23	103
MS.gene061578.t1	AT5G38120	25.424	472	265	13	18	438	43	478	6.34e-23	102
MS.gene061578.t1	AT5G38120	25.424	472	265	13	18	438	43	478	6.34e-23	102
MS.gene061578.t1	AT1G20510	26.000	450	235	18	18	410	36	444	3.04e-22	100
MS.gene061578.t1	AT3G16170	31.098	164	108	3	352	510	438	601	1.13e-18	90.1
MS.gene061578.t1	AT5G36880	32.335	167	104	3	352	513	503	665	9.22e-14	74.7
MS.gene061578.t1	AT5G36880	32.335	167	104	3	352	513	503	665	9.22e-14	74.7
MS.gene061578.t1	AT5G36880	32.335	167	104	3	352	513	503	665	9.22e-14	74.7
MS.gene061578.t1	AT5G36880	32.335	167	104	3	352	513	503	665	9.22e-14	74.7
MS.gene061578.t1	AT5G36880	32.335	167	104	3	352	513	553	715	9.83e-14	74.7
MS.gene061578.t1	AT1G64400	33.140	172	95	8	269	435	391	547	3.64e-13	72.8
MS.gene061578.t1	AT4G11030	32.418	182	102	9	260	435	381	547	8.74e-12	68.2
MS.gene061578.t1	AT1G77590	32.558	172	96	6	269	432	274	433	9.55e-12	68.2
MS.gene061578.t1	AT1G77590	32.558	172	96	6	269	432	420	579	1.06e-11	68.2
MS.gene061578.t1	AT4G23850	28.571	196	117	7	260	443	381	565	3.70e-11	66.2
MS.gene061578.t1	AT2G04350	29.658	263	140	12	269	514	449	683	4.57e-11	66.2
MS.gene061578.t1	AT2G04350	29.658	263	140	12	269	514	449	683	4.57e-11	66.2
MS.gene061578.t1	AT2G47240	25.291	344	214	14	100	426	160	477	7.05e-11	65.5
MS.gene061578.t1	AT2G47240	25.291	344	214	14	100	426	219	536	9.65e-11	65.1
MS.gene061578.t1	AT2G47240	25.291	344	214	14	100	426	219	536	9.65e-11	65.1
MS.gene061578.t1	AT2G47240	25.291	344	214	14	100	426	219	536	9.65e-11	65.1

Find 134 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGAATTTACCACACTTTA+TGG	0.126746	3.2:-59964931	MS.gene061578:CDS
TCTAAAGATGTGATTATTTC+AGG	0.185792	3.2:-59965149	MS.gene061578:CDS
CAAGGACTTTCACCCCATTT+TGG	0.195490	3.2:+59965038	None:intergenic
TATACATAAAGATGGTTATT+TGG	0.207931	3.2:-59965186	MS.gene061578:CDS
ATTTCTTCAGGAAGGTAATT+TGG	0.214710	3.2:+59965950	None:intergenic
ATCTTACCTGTTAAAGTCTT+TGG	0.233560	3.2:+59964876	None:intergenic
TTATGATTGACTTGATTATT+AGG	0.237452	3.2:+59964786	None:intergenic
GCTCTCATCAAGAAAGTTAA+AGG	0.239728	3.2:+59966809	None:intergenic
AAGCGACGGCGAAAGCTTTC+GGG	0.254708	3.2:-59965241	MS.gene061578:CDS
TTTAATGTAACACATGCTTA+TGG	0.280910	3.2:-59965500	MS.gene061578:CDS
GATTTACCAAAGACTTTAAC+AGG	0.288395	3.2:-59964882	MS.gene061578:CDS
TTGCAATGCCACATCCAAAA+TGG	0.293682	3.2:-59965052	MS.gene061578:CDS
AATAAAACAGGTATCAGTTT+TGG	0.306205	3.2:-59966287	MS.gene061578:intron
TGACTATAAATAACACCTTT+TGG	0.314032	3.2:+59965872	None:intergenic
TCATAATAATGACCAGATTA+TGG	0.314754	3.2:-59964769	MS.gene061578:CDS
GTATAGTTTGCTCGACATTT+TGG	0.327519	3.2:+59966842	None:intergenic
GAAAGTTAAAGGAGTGAGTA+TGG	0.331539	3.2:+59966820	None:intergenic
CATTCATCTTGAATTTCTTC+AGG	0.339619	3.2:+59965938	None:intergenic
GAAGCGACGGCGAAAGCTTT+CGG	0.344503	3.2:-59965242	MS.gene061578:CDS
TTTAATCCTTGGTTGGGAAA+TGG	0.345052	3.2:-59965822	MS.gene061578:CDS
TTCTTCCATGAGTAATCTTA+AGG	0.361126	3.2:+59966093	None:intergenic
GGTAATGTCCATAGATAAAC+TGG	0.364652	3.2:+59965791	None:intergenic
CGCATTGAGACGGTGGTTGA+TGG	0.387285	3.2:+59966198	None:intergenic
TCATACATTGCTGGAATGTT+TGG	0.387598	3.2:+59966262	None:intergenic
AAGTGAAACGGGTTCCTTCA+TGG	0.397922	3.2:+59966753	None:intergenic
TACCACAAAACACTTAGCTT+TGG	0.397967	3.2:+59964833	None:intergenic
TGGTAAATTCTTCCTACAAT+AGG	0.411253	3.2:+59964941	None:intergenic
TATGGACTAACTGAAGCAAC+AGG	0.418017	3.2:-59965482	MS.gene061578:CDS
GTAAGAGATGGGAAAACAAT+GGG	0.423096	3.2:-59965317	MS.gene061578:CDS
GATGTGTAATTCAATGTAAT+TGG	0.423792	3.2:+59965911	None:intergenic
TTTGAGTTTAATCCTTGGTT+GGG	0.424831	3.2:-59965828	MS.gene061578:CDS
TTAATCCTTGGTTGGGAAAT+GGG	0.425386	3.2:-59965821	MS.gene061578:CDS
TTTGTGAGTGGCAGAAGAAA+TGG	0.427336	3.2:-59965448	MS.gene061578:CDS
TTACCTATGTTCCATTGTAA+TGG	0.427373	3.2:-59965773	MS.gene061578:CDS
GAGCTCGCCTAGCCGGATTC+CGG	0.428306	3.2:+59966003	None:intergenic
TCTCCGGTGAATATACTAAC+TGG	0.428686	3.2:-59965572	MS.gene061578:CDS
CTTCATGGATGACTGAGGTT+CGG	0.429624	3.2:+59966768	None:intergenic
AAATGCATCTCATACATTGC+TGG	0.433221	3.2:+59966253	None:intergenic
GTTATTTGGAAATTAAAGAT+AGG	0.433555	3.2:-59965172	MS.gene061578:CDS
GACGGTGGTTGATGGTGTTT+AGG	0.435440	3.2:+59966206	None:intergenic
GACGAGATAGAGCCATAATC+TGG	0.444504	3.2:+59964757	None:intergenic
GTAGTGAACCAGTTTATCTA+TGG	0.450766	3.2:-59965799	MS.gene061578:CDS
TGCAATGCCACATCCAAAAT+GGG	0.459922	3.2:-59965051	MS.gene061578:CDS
GGGGATGGTTGGTTTAGAAC+TGG	0.460142	3.2:-59965221	MS.gene061578:CDS
ACACTTTATGGTACCTAAGA+TGG	0.462947	3.2:-59964919	MS.gene061578:CDS
GAGATTATGTTGAAGGGAAG+TGG	0.465461	3.2:-59965293	MS.gene061578:CDS
AGTTTGTGACCAAGTGAAAC+GGG	0.471117	3.2:+59966742	None:intergenic
CTGAAGAAATTCAAGATGAA+TGG	0.471358	3.2:-59965937	MS.gene061578:CDS
ATGGAGAGTGTGGTAAGAGA+TGG	0.472523	3.2:-59965329	MS.gene061578:CDS
TAAACACTCCCACCGGAATC+CGG	0.473541	3.2:-59966015	MS.gene061578:CDS
CTATGTTCCATTGTAATGGT+TGG	0.477230	3.2:-59965769	MS.gene061578:CDS
CTTTGAGTTTAATCCTTGGT+TGG	0.482972	3.2:-59965829	MS.gene061578:CDS
CGAAAGCTTTCGGGGATGGT+TGG	0.487803	3.2:-59965232	MS.gene061578:CDS
CGCCTCAGAGTGTCGAAGAA+TGG	0.487931	3.2:+59966162	None:intergenic
ACAGCAGCTTCTAAAACCTT+AGG	0.490308	3.2:+59965077	None:intergenic
AAGTTTGTGACCAAGTGAAA+CGG	0.494196	3.2:+59966741	None:intergenic
ACGGCGAAAGCTTTCGGGGA+TGG	0.495228	3.2:-59965236	MS.gene061578:CDS
TGCCACTCACAAACAAGTGC+TGG	0.499001	3.2:+59965458	None:intergenic
TGGTTTAGAACTGGTGATGT+TGG	0.499794	3.2:-59965212	MS.gene061578:CDS
ATGAGATAATTACCTATTGT+AGG	0.504104	3.2:-59964953	MS.gene061578:CDS
TGGAGAGTGTGGTAAGAGAT+GGG	0.505965	3.2:-59965328	MS.gene061578:CDS
ATTCGTATCAAAAGCAAAAG+AGG	0.506420	3.2:-59966116	MS.gene061578:CDS
AAGGGAAGTGGAATCATGAT+GGG	0.506638	3.2:-59965281	MS.gene061578:CDS
TGCAATATTGAGAGCACGGA+GGG	0.514867	3.2:+59966691	None:intergenic
CATCTTGAATTTCTTCAGGA+AGG	0.517226	3.2:+59965942	None:intergenic
AGGAAGCGAGGCGGCGACAC+CGG	0.518047	3.2:+59966714	None:intergenic
AGAGGCCTTAAGATTACTCA+TGG	0.518068	3.2:-59966098	MS.gene061578:CDS
CGTATTCGAGCTCGCCTAGC+CGG	0.519450	3.2:+59965996	None:intergenic
CTTGAGAAAATCGAGCCACT+TGG	0.519790	3.2:-59965524	MS.gene061578:CDS
ATGAAGGAACCCGTTTCACT+TGG	0.520277	3.2:-59966751	MS.gene061578:CDS
AGTACTTTGAGTTTAATCCT+TGG	0.521867	3.2:-59965833	MS.gene061578:CDS
CTCGCCTAGCCGGATTCCGG+TGG	0.524552	3.2:+59966006	None:intergenic
ATTACATTGAATTACACATC+AGG	0.525480	3.2:-59965908	MS.gene061578:CDS
CGAGCTCGAATACGAGCAAA+TGG	0.527362	3.2:-59965987	MS.gene061578:CDS
TGTTCTTTATAGTCATCCTA+AGG	0.533616	3.2:-59965093	MS.gene061578:CDS
CGATGCTTAAAGCGCGACAA+GGG	0.533885	3.2:-59965400	MS.gene061578:CDS
AACTGGTGGAGCACCACCCC+CGG	0.535495	3.2:-59965555	MS.gene061578:CDS
TGTGACACTAAGGAAGAATG+AGG	0.538924	3.2:-59965009	MS.gene061578:CDS
GAAGGGAAGTGGAATCATGA+TGG	0.539328	3.2:-59965282	MS.gene061578:CDS
GTTGGTGTTATACATAAAGA+TGG	0.545404	3.2:-59965194	MS.gene061578:CDS
AAAGAGAGTCATAAAATCTC+CGG	0.548039	3.2:-59965588	MS.gene061578:CDS
TCGAAGAATGGTGGCTATGT+TGG	0.554863	3.2:+59966174	None:intergenic
CAACCATTACAATGGAACAT+AGG	0.559310	3.2:+59965770	None:intergenic
CACTACCCATTTCCCAACCA+AGG	0.560736	3.2:+59965816	None:intergenic
CACGGAGGGAGGAAGCGAGG+CGG	0.564152	3.2:+59966705	None:intergenic
GGTACAACTTCAGAACCAAA+AGG	0.565976	3.2:-59965887	MS.gene061578:CDS
TCTTTGCAATATTGAGAGCA+CGG	0.566149	3.2:+59966687	None:intergenic
TACGAGCAAATGGTTCATCA+TGG	0.574224	3.2:-59965977	MS.gene061578:CDS
GTAATGGTTGGACATTCACT+TGG	0.575202	3.2:-59965757	MS.gene061578:CDS
CTTGGTCACAAACTTATGAC+CGG	0.576665	3.2:-59966733	MS.gene061578:CDS
CATCTATCACAATCACTAGT+GGG	0.580127	3.2:+59966041	None:intergenic
TAATGGTTGGACATTCACTT+GGG	0.583819	3.2:-59965756	MS.gene061578:CDS
GAGCACGGAGGGAGGAAGCG+AGG	0.588763	3.2:+59966702	None:intergenic
TCTTGAAACAATGGAGAGTG+TGG	0.595422	3.2:-59965339	MS.gene061578:CDS
GGCCAGCACTTGTTTGTGAG+TGG	0.595693	3.2:-59965460	MS.gene061578:CDS
TCATCTATCACAATCACTAG+TGG	0.596057	3.2:+59966040	None:intergenic
TGTGAAGAATCTTGAAACAA+TGG	0.596126	3.2:-59965348	MS.gene061578:CDS
ACTCCCACCGGAATCCGGCT+AGG	0.597247	3.2:-59966010	MS.gene061578:CDS
AGCGACGGCGAAAGCTTTCG+GGG	0.597981	3.2:-59965240	MS.gene061578:CDS
CGAACCTCAGTCATCCATGA+AGG	0.598539	3.2:-59966767	MS.gene061578:CDS
AAGAGATGGGAAAACAATGG+GGG	0.600740	3.2:-59965315	MS.gene061578:CDS
TTTCTCAAGTAGGGACGCCG+GGG	0.601501	3.2:+59965538	None:intergenic
CTATGTTGGCCGCATTGAGA+CGG	0.602111	3.2:+59966188	None:intergenic
TTTAGGACAGCACCAGCCAT+AGG	0.602558	3.2:+59966223	None:intergenic
TCGATGCTTAAAGCGCGACA+AGG	0.604868	3.2:-59965401	MS.gene061578:CDS
CCACCAGTTAGTATATTCAC+CGG	0.607871	3.2:+59965569	None:intergenic
CTCAGAGTGTCGAAGAATGG+TGG	0.608683	3.2:+59966165	None:intergenic
GGTGTTATTTATAGTCATAG+AGG	0.611582	3.2:-59965866	MS.gene061578:CDS
TGAATGTCCAACCATTACAA+TGG	0.613092	3.2:+59965762	None:intergenic
GGTAAGAGATGGGAAAACAA+TGG	0.619269	3.2:-59965318	MS.gene061578:CDS
ACTTGGGGTGTAGCTGCAAG+AGG	0.619778	3.2:-59965740	MS.gene061578:CDS
TGATTTATGTTGTGTTACTG+AGG	0.621798	3.2:-59964976	MS.gene061578:CDS
TAAGAGATGGGAAAACAATG+GGG	0.626561	3.2:-59965316	MS.gene061578:CDS
GATGCTTAAAGCGCGACAAG+GGG	0.626984	3.2:-59965399	MS.gene061578:CDS
TCGCCTAGCCGGATTCCGGT+GGG	0.628633	3.2:+59966007	None:intergenic
AATATTGAGAGCACGGAGGG+AGG	0.632194	3.2:+59966694	None:intergenic
GATGACATAAACACTCCCAC+CGG	0.634917	3.2:-59966022	MS.gene061578:CDS
CTTAGTGTCACAAAAGCACA+AGG	0.636960	3.2:+59965020	None:intergenic
TTCTCAAGTAGGGACGCCGG+GGG	0.637975	3.2:+59965539	None:intergenic
TGTTGGCCGCATTGAGACGG+TGG	0.638475	3.2:+59966191	None:intergenic
CACCATTCTTCGACACTCTG+AGG	0.640216	3.2:-59966164	MS.gene061578:CDS
TGTGTTACATTAAAACCAAG+TGG	0.640733	3.2:+59965509	None:intergenic
AATGGTTGGACATTCACTTG+GGG	0.644019	3.2:-59965755	MS.gene061578:CDS
CCGGTGAATATACTAACTGG+TGG	0.647354	3.2:-59965569	MS.gene061578:CDS
AAAGATGTGATTATTTCAGG+AGG	0.649768	3.2:-59965146	MS.gene061578:CDS
ATCTTAGGTACCATAAAGTG+TGG	0.661614	3.2:+59964921	None:intergenic
AGGGAAGTGGAATCATGATG+GGG	0.670807	3.2:-59965280	MS.gene061578:CDS
GACACTAAGGAAGAATGAGG+AGG	0.677688	3.2:-59965006	MS.gene061578:CDS
TGGGGTGTAGCTGCAAGAGG+AGG	0.680125	3.2:-59965737	MS.gene061578:intron
GGTTCCTTCATGGATGACTG+AGG	0.686748	3.2:+59966763	None:intergenic
ATGGACTAACTGAAGCAACA+GGG	0.714883	3.2:-59965481	MS.gene061578:CDS
TTGCAATATTGAGAGCACGG+AGG	0.733139	3.2:+59966690	None:intergenic
CATCAACCACCGTCTCAATG+CGG	0.733801	3.2:-59966197	MS.gene061578:CDS
GCAATGCCACATCCAAAATG+GGG	0.748056	3.2:-59965050	MS.gene061578:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATAATATTGAAAATGAT+GGG	+	chr3.2:59965980-59965999	None:intergenic	10.0%
!!	TAAAATAATATTGAAAATGA+TGG	+	chr3.2:59965981-59966000	None:intergenic	10.0%
!!!	ATCATTTTCAATATTATTTT+AGG	-	chr3.2:59965980-59965999	MS.gene061578:CDS	10.0%
!!	GCGATTTATAAAAACTAAAT+TGG	-	chr3.2:59965287-59965306	MS.gene061578:CDS	20.0%
!!	GTTATTTGGAAATTAAAGAT+AGG	-	chr3.2:59966434-59966453	MS.gene061578:intron	20.0%
!!	TATACATAAAGATGGTTATT+TGG	-	chr3.2:59966420-59966439	MS.gene061578:intron	20.0%
!!	TATTACAATATTTGATACTG+CGG	+	chr3.2:59965206-59965225	None:intergenic	20.0%
!!!	TGGTTTTTGTTTAATAAAAC+AGG	-	chr3.2:59965307-59965326	MS.gene061578:CDS	20.0%
!!!	TTATGATTGACTTGATTATT+AGG	+	chr3.2:59966823-59966842	None:intergenic	20.0%
!	ATGAGATAATTACCTATTGT+AGG	-	chr3.2:59966653-59966672	MS.gene061578:intron	25.0%
!	ATTACATTGAATTACACATC+AGG	-	chr3.2:59965698-59965717	MS.gene061578:intron	25.0%
!	CAAAATGCAGTATCAAATAT+TGG	+	chr3.2:59965182-59965201	None:intergenic	25.0%
!	GATGTGTAATTCAATGTAAT+TGG	+	chr3.2:59965698-59965717	None:intergenic	25.0%
!	TCATAATAATGACCAGATTA+TGG	-	chr3.2:59966837-59966856	MS.gene061578:CDS	25.0%
!	TCTAAAGATGTGATTATTTC+AGG	-	chr3.2:59966457-59966476	MS.gene061578:intron	25.0%
!	TGTCACATTATAGAGATTTA+TGG	+	chr3.2:59965942-59965961	None:intergenic	25.0%
!	TTTAATGTAACACATGCTTA+TGG	-	chr3.2:59966106-59966125	MS.gene061578:CDS	25.0%
!!	AATAAAACAGGTATCAGTTT+TGG	-	chr3.2:59965319-59965338	MS.gene061578:CDS	25.0%
!!	TGACTATAAATAACACCTTT+TGG	+	chr3.2:59965737-59965756	None:intergenic	25.0%
!!!	TTTTAAAGATAAAGAAGCGA+CGG	-	chr3.2:59966351-59966370	MS.gene061578:intron	25.0%
	AAAGAGAGTCATAAAATCTC+CGG	-	chr3.2:59966018-59966037	MS.gene061578:CDS	30.0%
	AAAGATGTGATTATTTCAGG+AGG	-	chr3.2:59966460-59966479	MS.gene061578:intron	30.0%
	ATCTTACCTGTTAAAGTCTT+TGG	+	chr3.2:59966733-59966752	None:intergenic	30.0%
	ATTCGTATCAAAAGCAAAAG+AGG	-	chr3.2:59965490-59965509	MS.gene061578:CDS	30.0%
	ATTTCTTCAGGAAGGTAATT+TGG	+	chr3.2:59965659-59965678	None:intergenic	30.0%
	CATTCATCTTGAATTTCTTC+AGG	+	chr3.2:59965671-59965690	None:intergenic	30.0%
	CTGAAGAAATTCAAGATGAA+TGG	-	chr3.2:59965669-59965688	MS.gene061578:intron	30.0%
	GAAGAATTTACCACACTTTA+TGG	-	chr3.2:59966675-59966694	MS.gene061578:intron	30.0%
	GATTTACCAAAGACTTTAAC+AGG	-	chr3.2:59966724-59966743	MS.gene061578:CDS	30.0%
	TCCATAAAAACAACCATCTT+AGG	+	chr3.2:59966703-59966722	None:intergenic	30.0%
	TGATATCATATCCTAGATTG+CGG	+	chr3.2:59965144-59965163	None:intergenic	30.0%
	TGGTAAATTCTTCCTACAAT+AGG	+	chr3.2:59966668-59966687	None:intergenic	30.0%
	TGTGAAGAATCTTGAAACAA+TGG	-	chr3.2:59966258-59966277	MS.gene061578:CDS	30.0%
	TGTGTTACATTAAAACCAAG+TGG	+	chr3.2:59966100-59966119	None:intergenic	30.0%
	TGTTCTTTATAGTCATCCTA+AGG	-	chr3.2:59966513-59966532	MS.gene061578:intron	30.0%
	TTACCTATGTTCCATTGTAA+TGG	-	chr3.2:59965833-59965852	MS.gene061578:CDS	30.0%
	TTCTTCCATGAGTAATCTTA+AGG	+	chr3.2:59965516-59965535	None:intergenic	30.0%
!	AGTACTTTGAGTTTAATCCT+TGG	-	chr3.2:59965773-59965792	MS.gene061578:CDS	30.0%
!	TGATTTATGTTGTGTTACTG+AGG	-	chr3.2:59966630-59966649	MS.gene061578:intron	30.0%
!	TTTGAGTTTAATCCTTGGTT+GGG	-	chr3.2:59965778-59965797	MS.gene061578:CDS	30.0%
!!	GGTGTTATTTATAGTCATAG+AGG	-	chr3.2:59965740-59965759	MS.gene061578:CDS	30.0%
!!	GTTGGTGTTATACATAAAGA+TGG	-	chr3.2:59966412-59966431	MS.gene061578:intron	30.0%
!!!	ACCTAAGATGGTTGTTTTTA+TGG	-	chr3.2:59966699-59966718	MS.gene061578:CDS	30.0%
!!!	TAAGATGGTTGTTTTTATGG+AGG	-	chr3.2:59966702-59966721	MS.gene061578:CDS	30.0%
	AAATGCATCTCATACATTGC+TGG	+	chr3.2:59965356-59965375	None:intergenic	35.0%
	AAGTTTGTGACCAAGTGAAA+CGG	+	chr3.2:59964868-59964887	None:intergenic	35.0%
	ACACTTTATGGTACCTAAGA+TGG	-	chr3.2:59966687-59966706	MS.gene061578:CDS	35.0%
	ATCTTAGGTACCATAAAGTG+TGG	+	chr3.2:59966688-59966707	None:intergenic	35.0%
	CAACCATTACAATGGAACAT+AGG	+	chr3.2:59965839-59965858	None:intergenic	35.0%
	CATCTATCACAATCACTAGT+GGG	+	chr3.2:59965568-59965587	None:intergenic	35.0%
	CATCTTGAATTTCTTCAGGA+AGG	+	chr3.2:59965667-59965686	None:intergenic	35.0%
	CTATGTTCCATTGTAATGGT+TGG	-	chr3.2:59965837-59965856	MS.gene061578:CDS	35.0%
	GAAAGTTAAAGGAGTGAGTA+TGG	+	chr3.2:59964789-59964808	None:intergenic	35.0%
	GCTCTCATCAAGAAAGTTAA+AGG	+	chr3.2:59964800-59964819	None:intergenic	35.0%
	GGTAATGTCCATAGATAAAC+TGG	+	chr3.2:59965818-59965837	None:intergenic	35.0%
	GTAAGAGATGGGAAAACAAT+GGG	-	chr3.2:59966289-59966308	MS.gene061578:intron	35.0%
	GTAGTGAACCAGTTTATCTA+TGG	-	chr3.2:59965807-59965826	MS.gene061578:CDS	35.0%
	TAAGAGATGGGAAAACAATG+GGG	-	chr3.2:59966290-59966309	MS.gene061578:intron	35.0%
	TAATGGTTGGACATTCACTT+GGG	-	chr3.2:59965850-59965869	MS.gene061578:CDS	35.0%
	TACCACAAAACACTTAGCTT+TGG	+	chr3.2:59966776-59966795	None:intergenic	35.0%
	TCATACATTGCTGGAATGTT+TGG	+	chr3.2:59965347-59965366	None:intergenic	35.0%
	TCATCTATCACAATCACTAG+TGG	+	chr3.2:59965569-59965588	None:intergenic	35.0%
	TCTTTGCAATATTGAGAGCA+CGG	+	chr3.2:59964922-59964941	None:intergenic	35.0%
	TGAATGTCCAACCATTACAA+TGG	+	chr3.2:59965847-59965866	None:intergenic	35.0%
	TTAATCCTTGGTTGGGAAAT+GGG	-	chr3.2:59965785-59965804	MS.gene061578:CDS	35.0%
	TTTAATCCTTGGTTGGGAAA+TGG	-	chr3.2:59965784-59965803	MS.gene061578:CDS	35.0%
!	CTTTGAGTTTAATCCTTGGT+TGG	-	chr3.2:59965777-59965796	MS.gene061578:CDS	35.0%
!	GCAATCTAGGATATGATATC+AGG	-	chr3.2:59965143-59965162	MS.gene061578:CDS	35.0%
!	TTTATGACTCTCTTTTCGCT+TGG	+	chr3.2:59966013-59966032	None:intergenic	35.0%
!!!	GTATAGTTTGCTCGACATTT+TGG	+	chr3.2:59964767-59964786	None:intergenic	35.0%
	AAGAGATGGGAAAACAATGG+GGG	-	chr3.2:59966291-59966310	MS.gene061578:intron	40.0%
	AAGGGAAGTGGAATCATGAT+GGG	-	chr3.2:59966325-59966344	MS.gene061578:intron	40.0%
	AATGGGGGAGATTATGTTGA+AGG	-	chr3.2:59966306-59966325	MS.gene061578:intron	40.0%
	AATGGTTGGACATTCACTTG+GGG	-	chr3.2:59965851-59965870	MS.gene061578:CDS	40.0%
	AGAGGCCTTAAGATTACTCA+TGG	-	chr3.2:59965508-59965527	MS.gene061578:CDS	40.0%
	AGTTTGTGACCAAGTGAAAC+GGG	+	chr3.2:59964867-59964886	None:intergenic	40.0%
	ATGGGGGAGATTATGTTGAA+GGG	-	chr3.2:59966307-59966326	MS.gene061578:intron	40.0%
	CCACCAGTTAGTATATTCAC+CGG	+	chr3.2:59966040-59966059	None:intergenic	40.0%
	CTTGGTCACAAACTTATGAC+CGG	-	chr3.2:59964873-59964892	MS.gene061578:CDS	40.0%
	GAGATTATGTTGAAGGGAAG+TGG	-	chr3.2:59966313-59966332	MS.gene061578:intron	40.0%
	GGTAAGAGATGGGAAAACAA+TGG	-	chr3.2:59966288-59966307	MS.gene061578:intron	40.0%
	GGTACAACTTCAGAACCAAA+AGG	-	chr3.2:59965719-59965738	MS.gene061578:intron	40.0%
	GTAATGGTTGGACATTCACT+TGG	-	chr3.2:59965849-59965868	MS.gene061578:CDS	40.0%
	TACGAGCAAATGGTTCATCA+TGG	-	chr3.2:59965629-59965648	MS.gene061578:intron	40.0%
	TCTCCGGTGAATATACTAAC+TGG	-	chr3.2:59966034-59966053	MS.gene061578:CDS	40.0%
	TCTTGAAACAATGGAGAGTG+TGG	-	chr3.2:59966267-59966286	MS.gene061578:CDS	40.0%
	TGCAATGCCACATCCAAAAT+GGG	-	chr3.2:59966555-59966574	MS.gene061578:intron	40.0%
	TGTGACACTAAGGAAGAATG+AGG	-	chr3.2:59966597-59966616	MS.gene061578:intron	40.0%
	TTGCAATGCCACATCCAAAA+TGG	-	chr3.2:59966554-59966573	MS.gene061578:intron	40.0%
	TTTGTGAGTGGCAGAAGAAA+TGG	-	chr3.2:59966158-59966177	MS.gene061578:CDS	40.0%
!	AGCCAAAGCTAAGTGTTTTG+TGG	-	chr3.2:59966771-59966790	MS.gene061578:CDS	40.0%
!	ATGGACTAACTGAAGCAACA+GGG	-	chr3.2:59966125-59966144	MS.gene061578:CDS	40.0%
!	CTTAGTGTCACAAAAGCACA+AGG	+	chr3.2:59966589-59966608	None:intergenic	40.0%
!	CTTGTGCTTTTGTGACACTA+AGG	-	chr3.2:59966587-59966606	MS.gene061578:intron	40.0%
!	GATGCATTTTGCAGTACCTA+TGG	-	chr3.2:59965367-59965386	MS.gene061578:CDS	40.0%
!	TATGGACTAACTGAAGCAAC+AGG	-	chr3.2:59966124-59966143	MS.gene061578:CDS	40.0%
!	TGGCTCGATTTTCTCAAGTA+GGG	+	chr3.2:59966080-59966099	None:intergenic	40.0%
!	TGGTTTAGAACTGGTGATGT+TGG	-	chr3.2:59966394-59966413	MS.gene061578:intron	40.0%
!!	ACAGCAGCTTCTAAAACCTT+AGG	+	chr3.2:59966532-59966551	None:intergenic	40.0%
!!	AGTTTGCTCGACATTTTGGT+AGG	+	chr3.2:59964763-59964782	None:intergenic	40.0%
!!!	AGCATCGATTGCTCTCTTTT+CGG	+	chr3.2:59966193-59966212	None:intergenic	40.0%
	AGGGAAGTGGAATCATGATG+GGG	-	chr3.2:59966326-59966345	MS.gene061578:intron	45.0%
	ATGAAGGAACCCGTTTCACT+TGG	-	chr3.2:59964855-59964874	MS.gene061578:CDS	45.0%
	CCGGTGAATATACTAACTGG+TGG	-	chr3.2:59966037-59966056	MS.gene061578:CDS	45.0%
	CTTCATGGATGACTGAGGTT+CGG	+	chr3.2:59964841-59964860	None:intergenic	45.0%
	CTTGAGAAAATCGAGCCACT+TGG	-	chr3.2:59966082-59966101	MS.gene061578:CDS	45.0%
	GAAGGGAAGTGGAATCATGA+TGG	-	chr3.2:59966324-59966343	MS.gene061578:intron	45.0%
	GACACTAAGGAAGAATGAGG+AGG	-	chr3.2:59966600-59966619	MS.gene061578:intron	45.0%
	GACGAGATAGAGCCATAATC+TGG	+	chr3.2:59966852-59966871	None:intergenic	45.0%
	GATGACATAAACACTCCCAC+CGG	-	chr3.2:59965584-59965603	MS.gene061578:CDS	45.0%
	GCAATGCCACATCCAAAATG+GGG	-	chr3.2:59966556-59966575	MS.gene061578:intron	45.0%
	TCGAAGAATGGTGGCTATGT+TGG	+	chr3.2:59965435-59965454	None:intergenic	45.0%
!	AAGTGAAACGGGTTCCTTCA+TGG	+	chr3.2:59964856-59964875	None:intergenic	45.0%
!	ATGGAGAGTGTGGTAAGAGA+TGG	-	chr3.2:59966277-59966296	MS.gene061578:CDS	45.0%
!	ATTTTCTCAAGTAGGGACGC+CGG	+	chr3.2:59966073-59966092	None:intergenic	45.0%
!	CAAGGACTTTCACCCCATTT+TGG	+	chr3.2:59966571-59966590	None:intergenic	45.0%
!	CATTTTGCAGTACCTATGGC+TGG	-	chr3.2:59965371-59965390	MS.gene061578:CDS	45.0%
!	CTTTCACCCCATTTTGGATG+TGG	+	chr3.2:59966565-59966584	None:intergenic	45.0%
!	GTGGCTCGATTTTCTCAAGT+AGG	+	chr3.2:59966081-59966100	None:intergenic	45.0%
!	TGCAATATTGAGAGCACGGA+GGG	+	chr3.2:59964918-59964937	None:intergenic	45.0%
!	TGGAGAGTGTGGTAAGAGAT+GGG	-	chr3.2:59966278-59966297	MS.gene061578:CDS	45.0%
!	TTGCAATATTGAGAGCACGG+AGG	+	chr3.2:59964919-59964938	None:intergenic	45.0%
	CACCATTCTTCGACACTCTG+AGG	-	chr3.2:59965442-59965461	MS.gene061578:CDS	50.0%
	CACTACCCATTTCCCAACCA+AGG	+	chr3.2:59965793-59965812	None:intergenic	50.0%
	CATCAACCACCGTCTCAATG+CGG	-	chr3.2:59965409-59965428	MS.gene061578:CDS	50.0%
	CGAACCTCAGTCATCCATGA+AGG	-	chr3.2:59964839-59964858	MS.gene061578:CDS	50.0%
	CGAGCTCGAATACGAGCAAA+TGG	-	chr3.2:59965619-59965638	MS.gene061578:intron	50.0%
	CGATGCTTAAAGCGCGACAA+GGG	-	chr3.2:59966206-59966225	MS.gene061578:CDS	50.0%
	CTATGTTGGCCGCATTGAGA+CGG	+	chr3.2:59965421-59965440	None:intergenic	50.0%
	GATGCTTAAAGCGCGACAAG+GGG	-	chr3.2:59966207-59966226	MS.gene061578:CDS	50.0%
	GGTTCCTTCATGGATGACTG+AGG	+	chr3.2:59964846-59964865	None:intergenic	50.0%
	TAAACACTCCCACCGGAATC+CGG	-	chr3.2:59965591-59965610	MS.gene061578:CDS	50.0%
	TAGATCAGCGACCGCAATCT+AGG	-	chr3.2:59965130-59965149	MS.gene061578:CDS	50.0%
	TAGATTGCGGTCGCTGATCT+AGG	+	chr3.2:59965131-59965150	None:intergenic	50.0%
	TCGATGCTTAAAGCGCGACA+AGG	-	chr3.2:59966205-59966224	MS.gene061578:CDS	50.0%
	TGCCACTCACAAACAAGTGC+TGG	+	chr3.2:59966151-59966170	None:intergenic	50.0%
!	AATATTGAGAGCACGGAGGG+AGG	+	chr3.2:59964915-59964934	None:intergenic	50.0%
!	TTTAGGACAGCACCAGCCAT+AGG	+	chr3.2:59965386-59965405	None:intergenic	50.0%
!	TTTTCTCAAGTAGGGACGCC+GGG	+	chr3.2:59966072-59966091	None:intergenic	50.0%
!!	CTCAGAGTGTCGAAGAATGG+TGG	+	chr3.2:59965444-59965463	None:intergenic	50.0%
!!	GACGGTGGTTGATGGTGTTT+AGG	+	chr3.2:59965403-59965422	None:intergenic	50.0%
!!	GGGGATGGTTGGTTTAGAAC+TGG	-	chr3.2:59966385-59966404	MS.gene061578:intron	50.0%
	AAGCGACGGCGAAAGCTTTC+GGG	-	chr3.2:59966365-59966384	MS.gene061578:intron	55.0%
	ACTTGGGGTGTAGCTGCAAG+AGG	-	chr3.2:59965866-59965885	MS.gene061578:CDS	55.0%
	CGAAAGCTTTCGGGGATGGT+TGG	-	chr3.2:59966374-59966393	MS.gene061578:intron	55.0%
	CGCATTGAGACGGTGGTTGA+TGG	+	chr3.2:59965411-59965430	None:intergenic	55.0%
	GAAGCGACGGCGAAAGCTTT+CGG	-	chr3.2:59966364-59966383	MS.gene061578:intron	55.0%
	GGCCAGCACTTGTTTGTGAG+TGG	-	chr3.2:59966146-59966165	MS.gene061578:CDS	55.0%
	TTTCTCAAGTAGGGACGCCG+GGG	+	chr3.2:59966071-59966090	None:intergenic	55.0%
!!	CGCCTCAGAGTGTCGAAGAA+TGG	+	chr3.2:59965447-59965466	None:intergenic	55.0%
	ACGGCGAAAGCTTTCGGGGA+TGG	-	chr3.2:59966370-59966389	MS.gene061578:intron	60.0%
	AGCGACGGCGAAAGCTTTCG+GGG	-	chr3.2:59966366-59966385	MS.gene061578:intron	60.0%
	CGTATTCGAGCTCGCCTAGC+CGG	+	chr3.2:59965613-59965632	None:intergenic	60.0%
	TGGGGTGTAGCTGCAAGAGG+AGG	-	chr3.2:59965869-59965888	MS.gene061578:CDS	60.0%
	TGTTGGCCGCATTGAGACGG+TGG	+	chr3.2:59965418-59965437	None:intergenic	60.0%
	TTCTCAAGTAGGGACGCCGG+GGG	+	chr3.2:59966070-59966089	None:intergenic	60.0%
	ACTCCCACCGGAATCCGGCT+AGG	-	chr3.2:59965596-59965615	MS.gene061578:CDS	65.0%
	GAGCTCGCCTAGCCGGATTC+CGG	+	chr3.2:59965606-59965625	None:intergenic	65.0%
	TCGCCTAGCCGGATTCCGGT+GGG	+	chr3.2:59965602-59965621	None:intergenic	65.0%
!	AACTGGTGGAGCACCACCCC+CGG	-	chr3.2:59966051-59966070	MS.gene061578:CDS	65.0%
	AGGAAGCGAGGCGGCGACAC+CGG	+	chr3.2:59964895-59964914	None:intergenic	70.0%
	CACGGAGGGAGGAAGCGAGG+CGG	+	chr3.2:59964904-59964923	None:intergenic	70.0%
	CTCGCCTAGCCGGATTCCGG+TGG	+	chr3.2:59965603-59965622	None:intergenic	70.0%
	GAGCACGGAGGGAGGAAGCG+AGG	+	chr3.2:59964907-59964926	None:intergenic	70.0%
	TCAAGTAGGGACGCCGGGGG+TGG	+	chr3.2:59966067-59966086	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	59964752	59966876	59964752	ID=MS.gene061578
chr3.2	mRNA	59964752	59966876	59964752	ID=MS.gene061578.t1;Parent=MS.gene061578
chr3.2	exon	59966679	59966876	59966679	ID=MS.gene061578.t1.exon1;Parent=MS.gene061578.t1
chr3.2	CDS	59966679	59966876	59966679	ID=cds.MS.gene061578.t1;Parent=MS.gene061578.t1
chr3.2	exon	59965738	59966299	59965738	ID=MS.gene061578.t1.exon2;Parent=MS.gene061578.t1
chr3.2	CDS	59965738	59966299	59965738	ID=cds.MS.gene061578.t1;Parent=MS.gene061578.t1
chr3.2	exon	59964752	59965626	59964752	ID=MS.gene061578.t1.exon3;Parent=MS.gene061578.t1
chr3.2	CDS	59964752	59965626	59964752	ID=cds.MS.gene061578.t1;Parent=MS.gene061578.t1

Gene Sequence

>MS.gene061578

ATGGATAACCTACCAAAATGTCGAGCAAACTATACCATACTCACTCCTTTAACTTTCTTGATGAGAGCTTCTGCATCTTATGCAAACCGAACCTCAGTCATCCATGAAGGAACCCGTTTCACTTGGTCACAAACTTATGACCGGTGTCGCCGCCTCGCTTCCTCCCTCCGTGCTCTCAATATTGCAAAGAATGATGTTGTAAGTTCGTCACTAAATCGTGTTTTTCAAGTAGTACACAAACATCAAAGTATATAAGTATTCAAATCTCAATAAAAATTCATGCATTCATATATCTTGTATTTGATTGCAATTTTTTTGCATTATCGAGTACTGTGACTTAAACGAGATTGCAATTTAAAACGTTACATGTTCATTTCCTAGATCAGCGACCGCAATCTAGGATATGATATCAGGTAAAATTGTATTGCCAATATTTGATACTGCATTTTGTCCGCAGTATCAAATATTGTAATAATACACTAACTCAACGTGACTACAATTCAAAATGTTACATACTATTATGATTAAGATTCGCGCGATTTATAAAAACTAAATTGGTTTTTGTTTAATAAAACAGGTATCAGTTTTGGCACCAAACATTCCAGCAATGTATGAGATGCATTTTGCAGTACCTATGGCTGGTGCTGTCCTAAACACCATCAACCACCGTCTCAATGCGGCCAACATAGCCACCATTCTTCGACACTCTGAGGCGAAAGTCTTCTTCGTAGACTACGAATTCGTATCAAAAGCAAAAGAGGCCTTAAGATTACTCATGGAAGAAAAAGATCAAAAAGAACAATACTCATCACTCCCACTAGTGATTGTGATAGATGACATAAACACTCCCACCGGAATCCGGCTAGGCGAGCTCGAATACGAGCAAATGGTTCATCATGGTAATCCAAATTACCTTCCTGAAGAAATTCAAGATGAATGGTCACCAATTACATTGAATTACACATCAGGTACAACTTCAGAACCAAAAGGTGTTATTTATAGTCATAGAGGAGCTTATTTAAGTACTTTGAGTTTAATCCTTGGTTGGGAAATGGGTAGTGAACCAGTTTATCTATGGACATTACCTATGTTCCATTGTAATGGTTGGACATTCACTTGGGGTGTAGCTGCAAGAGGAGGTACTAATATTTGTATCAGAAACACTGCAGCATCTGATATATATAGAGCCATAAATCTCTATAATGTGACACACATGTGTTGCGCACCCATCATTTTCAATATTATTTTAGGCGCGAAACCAAGCGAAAAGAGAGTCATAAAATCTCCGGTGAATATACTAACTGGTGGAGCACCACCCCCGGCGTCCCTACTTGAGAAAATCGAGCCACTTGGTTTTAATGTAACACATGCTTATGGACTAACTGAAGCAACAGGGCCAGCACTTGTTTGTGAGTGGCAGAAGAAATGGAATGTGTTACCGAAAAGAGAGCAATCGATGCTTAAAGCGCGACAAGGGGTTAGTGTTTTGACACTTGCTGATGTTGATGTGAAGAATCTTGAAACAATGGAGAGTGTGGTAAGAGATGGGAAAACAATGGGGGAGATTATGTTGAAGGGAAGTGGAATCATGATGGGGTATTTTAAAGATAAAGAAGCGACGGCGAAAGCTTTCGGGGATGGTTGGTTTAGAACTGGTGATGTTGGTGTTATACATAAAGATGGTTATTTGGAAATTAAAGATAGGTCTAAAGATGTGATTATTTCAGGAGGTGAAAATATTAGTAGTGTTGAAGTTGAAAATGTTCTTTATAGTCATCCTAAGGTTTTAGAAGCTGCTGTTGTTGCAATGCCACATCCAAAATGGGGTGAAAGTCCTTGTGCTTTTGTGACACTAAGGAAGAATGAGGAGGAAAAAAGTGATTTATGTTGTGTTACTGAGGATGAGATAATTACCTATTGTAGGAAGAATTTACCACACTTTATGGTACCTAAGATGGTTGTTTTTATGGAGGATTTACCAAAGACTTTAACAGGTAAGATTCAGAAATTTGAATTAAGAGCCAAAGCTAAGTGTTTTGTGGTAAATGATGAGAAGAATAAGAAGAAGCCTAATAATCAAGTCAATCATAATAATGACCAGATTATGGCTCTATCTCGTCTTTGA

Protein sequence

>MS.gene061578.t1

MDNLPKCRANYTILTPLTFLMRASASYANRTSVIHEGTRFTWSQTYDRCRRLASSLRALNIAKNDVVSVLAPNIPAMYEMHFAVPMAGAVLNTINHRLNAANIATILRHSEAKVFFVDYEFVSKAKEALRLLMEEKDQKEQYSSLPLVIVIDDINTPTGIRLGELEYEQMVHHGNPNYLPEEIQDEWSPITLNYTSGTTSEPKGVIYSHRGAYLSTLSLILGWEMGSEPVYLWTLPMFHCNGWTFTWGVAARGGAKPSEKRVIKSPVNILTGGAPPPASLLEKIEPLGFNVTHAYGLTEATGPALVCEWQKKWNVLPKREQSMLKARQGVSVLTLADVDVKNLETMESVVRDGKTMGEIMLKGSGIMMGYFKDKEATAKAFGDGWFRTGDVGVIHKDGYLEIKDRSKDVIISGGENISSVEVENVLYSHPKVLEAAVVAMPHPKWGESPCAFVTLRKNEEEKSDLCCVTEDEIITYCRKNLPHFMVPKMVVFMEDLPKTLTGKIQKFELRAKAKCFVVNDEKNKKKPNNQVNHNNDQIMALSRL