Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76405.t1 | XP_003629418.1 | 97.8 | 314 | 7 | 0 | 1 | 314 | 1 | 314 | 4.30E-171 | 610.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76405.t1 | Q8IUS5 | 24.9 | 265 | 176 | 6 | 61 | 305 | 93 | 354 | 8.6e-11 | 69.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76405.t1 | G7LDX9 | 97.8 | 314 | 7 | 0 | 1 | 314 | 1 | 314 | 3.1e-171 | 610.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049281 | MS.gene76405 | 0.801928 | 7.14E-49 | -1.69E-46 |
| MS.gene050082 | MS.gene76405 | 0.857315 | 1.78E-62 | -1.69E-46 |
| MS.gene050521 | MS.gene76405 | 0.820157 | 7.96E-53 | -1.69E-46 |
| MS.gene050832 | MS.gene76405 | 0.833664 | 4.68E-56 | -1.69E-46 |
| MS.gene051364 | MS.gene76405 | 0.831068 | 2.06E-55 | -1.69E-46 |
| MS.gene051366 | MS.gene76405 | 0.829529 | 4.89E-55 | -1.69E-46 |
| MS.gene051367 | MS.gene76405 | 0.849976 | 2.29E-60 | -1.69E-46 |
| MS.gene051422 | MS.gene76405 | 0.84281 | 2.06E-58 | -1.69E-46 |
| MS.gene053957 | MS.gene76405 | 0.80605 | 9.93E-50 | -1.69E-46 |
| MS.gene053958 | MS.gene76405 | 0.801358 | 9.35E-49 | -1.69E-46 |
| MS.gene055942 | MS.gene76405 | 0.8218 | 3.33E-53 | -1.69E-46 |
| MS.gene056761 | MS.gene76405 | 0.823039 | 1.72E-53 | -1.69E-46 |
| MS.gene057607 | MS.gene76405 | 0.802991 | 4.31E-49 | -1.69E-46 |
| MS.gene058015 | MS.gene76405 | 0.811707 | 6.12E-51 | -1.69E-46 |
| MS.gene058111 | MS.gene76405 | 0.839064 | 1.98E-57 | -1.69E-46 |
| MS.gene058268 | MS.gene76405 | 0.805311 | 1.42E-49 | -1.69E-46 |
| MS.gene058347 | MS.gene76405 | 0.805508 | 1.29E-49 | -1.69E-46 |
| MS.gene058349 | MS.gene76405 | 0.812545 | 4.02E-51 | -1.69E-46 |
| MS.gene058431 | MS.gene76405 | 0.80872 | 2.70E-50 | -1.69E-46 |
| MS.gene059770 | MS.gene76405 | 0.805452 | 1.33E-49 | -1.69E-46 |
| MS.gene060823 | MS.gene76405 | 0.817477 | 3.23E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76405.t1 | MTR_8g077290 | 97.771 | 314 | 7 | 0 | 1 | 314 | 1 | 314 | 0.0 | 635 |
| MS.gene76405.t1 | MTR_8g077290 | 98.155 | 271 | 5 | 0 | 1 | 271 | 1 | 271 | 0.0 | 548 |
| MS.gene76405.t1 | MTR_5g020260 | 64.495 | 307 | 106 | 1 | 1 | 307 | 1 | 304 | 1.74e-151 | 427 |
| MS.gene76405.t1 | MTR_5g020250 | 64.401 | 309 | 107 | 1 | 1 | 309 | 1 | 306 | 1.19e-149 | 423 |
| MS.gene76405.t1 | MTR_5g020320 | 62.460 | 309 | 107 | 1 | 1 | 309 | 1 | 300 | 1.69e-147 | 417 |
| MS.gene76405.t1 | MTR_3g105290 | 37.676 | 284 | 158 | 5 | 26 | 305 | 32 | 300 | 1.10e-59 | 193 |
| MS.gene76405.t1 | MTR_3g087050 | 34.483 | 290 | 172 | 4 | 19 | 305 | 24 | 298 | 1.58e-52 | 175 |
| MS.gene76405.t1 | MTR_2g008880 | 33.968 | 315 | 175 | 9 | 2 | 305 | 9 | 301 | 1.10e-49 | 168 |
| MS.gene76405.t1 | MTR_5g064035 | 31.104 | 299 | 176 | 6 | 19 | 307 | 22 | 300 | 3.46e-46 | 158 |
| MS.gene76405.t1 | MTR_3g105290 | 38.636 | 220 | 117 | 4 | 26 | 242 | 32 | 236 | 5.95e-44 | 150 |
| MS.gene76405.t1 | MTR_8g090060 | 28.179 | 291 | 157 | 5 | 19 | 305 | 22 | 264 | 6.36e-35 | 128 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76405.t1 | AT2G18360 | 68.051 | 313 | 99 | 1 | 1 | 312 | 1 | 313 | 7.40e-163 | 456 |
| MS.gene76405.t1 | AT4G36610 | 66.349 | 315 | 105 | 1 | 1 | 315 | 1 | 314 | 1.85e-152 | 430 |
| MS.gene76405.t1 | AT2G18360 | 67.279 | 272 | 88 | 1 | 1 | 271 | 1 | 272 | 2.24e-138 | 392 |
| MS.gene76405.t1 | AT5G21950 | 36.054 | 294 | 170 | 6 | 19 | 309 | 23 | 301 | 4.49e-56 | 184 |
| MS.gene76405.t1 | AT5G21950 | 36.054 | 294 | 170 | 6 | 19 | 309 | 75 | 353 | 2.09e-55 | 184 |
| MS.gene76405.t1 | AT4G33180 | 33.898 | 295 | 179 | 5 | 16 | 305 | 21 | 304 | 1.40e-54 | 180 |
| MS.gene76405.t1 | AT1G78210 | 34.667 | 300 | 167 | 7 | 17 | 305 | 20 | 301 | 3.60e-52 | 174 |
| MS.gene76405.t1 | AT5G21950 | 35.985 | 264 | 155 | 5 | 11 | 271 | 15 | 267 | 2.95e-50 | 168 |
| MS.gene76405.t1 | AT4G39955 | 32.215 | 298 | 173 | 7 | 19 | 307 | 22 | 299 | 8.17e-46 | 158 |
| MS.gene76405.t1 | AT5G09430 | 30.671 | 313 | 190 | 7 | 3 | 305 | 12 | 307 | 1.39e-43 | 152 |
| MS.gene76405.t1 | AT1G72620 | 30.847 | 295 | 175 | 9 | 21 | 305 | 56 | 331 | 1.17e-42 | 150 |
| MS.gene76405.t1 | AT1G17430 | 30.519 | 308 | 177 | 10 | 15 | 307 | 46 | 331 | 9.40e-40 | 142 |
| MS.gene76405.t1 | AT4G33180 | 34.783 | 207 | 121 | 3 | 16 | 219 | 21 | 216 | 7.48e-36 | 129 |
| MS.gene76405.t1 | AT5G21950 | 34.906 | 212 | 126 | 3 | 11 | 221 | 15 | 215 | 7.07e-35 | 126 |
| MS.gene76405.t1 | AT5G21950 | 34.906 | 212 | 126 | 3 | 11 | 221 | 15 | 215 | 7.07e-35 | 126 |
| MS.gene76405.t1 | AT5G21950 | 34.906 | 212 | 126 | 3 | 11 | 221 | 67 | 267 | 2.06e-34 | 127 |
| MS.gene76405.t1 | AT5G21950 | 34.906 | 212 | 126 | 3 | 11 | 221 | 67 | 267 | 2.06e-34 | 127 |
Find 55 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATTACCTGGGTGAAATT+TGG | 0.139926 | 8.1:-30336521 | None:intergenic |
| TTGTTACCTAATTCTGTTAA+AGG | 0.173805 | 8.1:+30333846 | MS.gene76405:CDS |
| AGAACAACCACGGGTTTATT+TGG | 0.190190 | 8.1:-30333465 | None:intergenic |
| GGTTATATCTGGATCCATTT+TGG | 0.210415 | 8.1:+30333758 | MS.gene76405:CDS |
| TTAAGTCCTTTAACAGAATT+AGG | 0.213404 | 8.1:-30333852 | None:intergenic |
| GTTCAAGCTTTGGTTATATC+TGG | 0.227268 | 8.1:+30333747 | MS.gene76405:CDS |
| TACAGAGGATACATCTTCTA+TGG | 0.263240 | 8.1:+30342105 | MS.gene76405:intron |
| GAAGTTTATTGCTTCCTTCT+TGG | 0.277735 | 8.1:+30342845 | MS.gene76405:CDS |
| TCCTGAATTGGTTCAAGCTT+TGG | 0.318085 | 8.1:+30333737 | MS.gene76405:CDS |
| ACCAAAGCTTGAACCAATTC+AGG | 0.325503 | 8.1:-30333738 | None:intergenic |
| TGTAGGTGATGTTCACAAAT+AGG | 0.341498 | 8.1:+30336441 | MS.gene76405:intron |
| AGAATCAGTCATAGCCAAAA+TGG | 0.352662 | 8.1:-30333772 | None:intergenic |
| AGTGTTTAGCCATTGCTTTA+AGG | 0.362247 | 8.1:+30333637 | MS.gene76405:CDS |
| GTTCCAGATCTTCTATTCTT+CGG | 0.384407 | 8.1:+30333567 | MS.gene76405:CDS |
| CCCGTGGTTGTTCTAGTACA+TGG | 0.391325 | 8.1:+30333474 | MS.gene76405:CDS |
| GATGCATGGGCTAATGAAAA+TGG | 0.402441 | 8.1:+30333323 | MS.gene76405:CDS |
| GAACGAGGTGAGCTATTAGA+TGG | 0.417602 | 8.1:+30336467 | MS.gene76405:CDS |
| CCTCCGAAGAATAGAAGATC+TGG | 0.418433 | 8.1:-30333570 | None:intergenic |
| TTCATTAGCCCATGCATCAA+TGG | 0.424084 | 8.1:-30333318 | None:intergenic |
| GCCATTGCTTTAAGGAAACT+TGG | 0.430633 | 8.1:+30333645 | MS.gene76405:CDS |
| AGCTGGTCACCTTGTTCACT+TGG | 0.434706 | 8.1:+30342791 | MS.gene76405:CDS |
| GTGAGTTCATTGCAAGAACT+TGG | 0.443234 | 8.1:+30333801 | MS.gene76405:CDS |
| GGCTGAGATGTATCCTGAAT+TGG | 0.452248 | 8.1:+30333725 | MS.gene76405:CDS |
| AAGTCTTTGTGTAAACGATC+CGG | 0.465183 | 8.1:-30333915 | None:intergenic |
| GGCTTTGTTATGGTCTCTGA+AGG | 0.474712 | 8.1:-30333405 | None:intergenic |
| TGATTCTATCTCTACTGTGT+AGG | 0.492993 | 8.1:-30333358 | None:intergenic |
| GGAGAGGGCACAACGTTTGA+AGG | 0.498839 | 8.1:+30342759 | MS.gene76405:CDS |
| TACACAGTAGAGATAGAATC+AGG | 0.501725 | 8.1:+30333360 | MS.gene76405:CDS |
| ACAGAGGATACATCTTCTAT+GGG | 0.506859 | 8.1:+30342106 | MS.gene76405:intron |
| GATTCTATCTCTACTGTGTA+GGG | 0.513782 | 8.1:-30333357 | None:intergenic |
| TTTGAAGGTATAAAGAAAGC+TGG | 0.532621 | 8.1:+30342774 | MS.gene76405:CDS |
| ACCAAGTTTCCTTAAAGCAA+TGG | 0.540210 | 8.1:-30333646 | None:intergenic |
| AAACACTCTGCTTGAAACCT+AGG | 0.543493 | 8.1:-30333621 | None:intergenic |
| AGTCTTTGTGTAAACGATCC+GGG | 0.560029 | 8.1:-30333914 | None:intergenic |
| GTATCAAATGACAGGCAACT+AGG | 0.560612 | 8.1:+30342738 | MS.gene76405:intron |
| CCAGATCTTCTATTCTTCGG+AGG | 0.563133 | 8.1:+30333570 | MS.gene76405:CDS |
| CCATGTTACTATTCCTTCAG+AGG | 0.572953 | 8.1:-30333502 | None:intergenic |
| TGCTTCATTTGACGCCAAGA+AGG | 0.573642 | 8.1:-30342859 | None:intergenic |
| CATGGGCTAATGAAAATGGC+AGG | 0.587155 | 8.1:+30333327 | MS.gene76405:CDS |
| AAATGACAGGCAACTAGGAG+AGG | 0.595528 | 8.1:+30342743 | MS.gene76405:intron |
| AGAAATCATGGTGAATCTTG+TGG | 0.599888 | 8.1:+30333284 | None:intergenic |
| ATGGTCGCTCCAAGTGAACA+AGG | 0.615675 | 8.1:-30342800 | None:intergenic |
| GTAACATGGCAGTTTCAAGT+TGG | 0.615961 | 8.1:+30333516 | MS.gene76405:CDS |
| CCTCTGAAGGAATAGTAACA+TGG | 0.616910 | 8.1:+30333502 | MS.gene76405:CDS |
| CCATGTACTAGAACAACCAC+GGG | 0.619067 | 8.1:-30333474 | None:intergenic |
| TTTACACAAAGACTTTCTTG+AGG | 0.635532 | 8.1:+30333923 | MS.gene76405:CDS |
| ACCATGTACTAGAACAACCA+CGG | 0.639608 | 8.1:-30333475 | None:intergenic |
| TGCAAAACCAAATAAACCCG+TGG | 0.661215 | 8.1:+30333458 | MS.gene76405:CDS |
| AATGACAGGCAACTAGGAGA+GGG | 0.661901 | 8.1:+30342744 | MS.gene76405:intron |
| AGACATCTATTGTAGACACA+TGG | 0.683319 | 8.1:-30342819 | None:intergenic |
| CAACATTCCAAATTTCACCC+AGG | 0.683392 | 8.1:+30336514 | MS.gene76405:CDS |
| CAGAGGATACATCTTCTATG+GGG | 0.694300 | 8.1:+30342107 | MS.gene76405:intron |
| ACAAAACAGAAAGATCACCT+AGG | 0.708866 | 8.1:+30333604 | MS.gene76405:CDS |
| ATTCTTCGGAGGTTCAACCA+CGG | 0.719626 | 8.1:+30333581 | MS.gene76405:CDS |
| TTCACAAATAGGAAAGAACG+AGG | 0.731188 | 8.1:+30336452 | MS.gene76405:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 30333291 | 30342887 | 30333291 | ID=MS.gene76405 |
| chr8.1 | mRNA | 30333291 | 30342887 | 30333291 | ID=MS.gene76405.t1;Parent=MS.gene76405 |
| chr8.1 | exon | 30333291 | 30333944 | 30333291 | ID=MS.gene76405.t1.exon1;Parent=MS.gene76405.t1 |
| chr8.1 | CDS | 30333291 | 30333944 | 30333291 | ID=cds.MS.gene76405.t1;Parent=MS.gene76405.t1 |
| chr8.1 | exon | 30336446 | 30336535 | 30336446 | ID=MS.gene76405.t1.exon2;Parent=MS.gene76405.t1 |
| chr8.1 | CDS | 30336446 | 30336535 | 30336446 | ID=cds.MS.gene76405.t1;Parent=MS.gene76405.t1 |
| chr8.1 | exon | 30342110 | 30342177 | 30342110 | ID=MS.gene76405.t1.exon3;Parent=MS.gene76405.t1 |
| chr8.1 | CDS | 30342110 | 30342177 | 30342110 | ID=cds.MS.gene76405.t1;Parent=MS.gene76405.t1 |
| chr8.1 | exon | 30342752 | 30342887 | 30342752 | ID=MS.gene76405.t1.exon4;Parent=MS.gene76405.t1 |
| chr8.1 | CDS | 30342752 | 30342887 | 30342752 | ID=cds.MS.gene76405.t1;Parent=MS.gene76405.t1 |
| Gene Sequence |
| Protein sequence |
