AlfalfaGEDB | MS.gene061528

M. sativa cultivar XinJiangDaYe / MS.gene061528

KEGG: K09264
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0110165 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF01486.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061528.t1	KEH28130.1	98.4	125	2	0	1	125	55	179	5.10E-39	170.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061528.t1	Q38840	64.2	120	39	1	2	121	112	227	2.3e-11	70.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061528.t1	A0A072UQJ7	98.4	125	2	0	1	125	55	179	3.7e-39	170.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene78610	MS.gene061528	PPI
MS.gene069104	MS.gene061528	PPI
MS.gene061528	MS.gene53154	PPI
MS.gene027784	MS.gene061528	PPI
MS.gene061528	MS.gene86241	PPI
MS.gene55353	MS.gene061528	PPI
MS.gene85927	MS.gene061528	PPI
MS.gene061528	MS.gene046410	PPI
MS.gene061528	MS.gene44269	PPI
MS.gene070679	MS.gene061528	PPI
MS.gene061528	MS.gene027750	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061528.t1	MTR_5g066990	97.600	125	3	0	1	125	55	179	1.64e-85	248
MS.gene061528.t1	MTR_5g066990	87.200	125	2	1	1	125	55	165	1.53e-71	212
MS.gene061528.t1	MTR_2g009890	47.183	142	75	0	1	142	111	252	1.75e-38	131
MS.gene061528.t1	MTR_5g031000	55.906	127	50	3	1	122	110	235	5.14e-37	127
MS.gene061528.t1	MTR_5g031000	49.606	127	44	4	1	122	110	221	3.61e-28	103
MS.gene061528.t1	MTR_4g092990	42.342	111	63	1	1	111	28	137	1.50e-20	82.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061528.t1	AT3G57230	52.273	132	61	2	1	132	77	206	6.76e-38	128
MS.gene061528.t1	AT3G57230	52.273	132	61	2	1	132	77	206	6.76e-38	128
MS.gene061528.t1	AT3G57230	52.273	132	61	2	1	132	111	240	2.51e-37	127
MS.gene061528.t1	AT3G57230	52.273	132	61	2	1	132	111	240	2.51e-37	127
MS.gene061528.t1	AT2G22630	51.667	120	54	1	2	121	112	227	2.49e-33	117
MS.gene061528.t1	AT2G22630	51.667	120	54	1	2	121	112	227	2.49e-33	117
MS.gene061528.t1	AT4G37940	49.153	118	55	1	1	118	50	162	1.55e-31	110
MS.gene061528.t1	AT4G37940	49.153	118	55	1	1	118	111	223	4.07e-31	111
MS.gene061528.t1	AT2G14210	49.107	112	52	2	1	111	112	219	2.23e-22	89.0
MS.gene061528.t1	AT2G14210	49.107	112	52	2	1	111	112	219	2.50e-22	89.0
MS.gene061528.t1	AT4G37940	42.373	118	49	2	1	118	111	209	5.00e-22	87.8

Find 22 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGTGGTGTTCGTATGAAAA+AGG	0.265336	5.4:+52266643	MS.gene061528:CDS
TTTGACAGTCAAAGAATTAC+AGG	0.270267	5.4:+52266592	MS.gene061528:CDS
GTTGCTGTGGCTGGCTGAGC+TGG	0.334824	5.4:-52267192	None:intergenic
AGTCAAAGAATTACAGGGTT+TGG	0.349043	5.4:+52266598	MS.gene061528:CDS
TGTAGTGTTGTTGCTGTGGC+TGG	0.360947	5.4:-52267201	None:intergenic
CAACACTACAAGGCCCCTTC+AGG	0.412465	5.4:+52267214	MS.gene061528:CDS
TGGAGATTCACAGGTACATT+TGG	0.417965	5.4:-52267172	None:intergenic
CACGAAGGCTGATTTCCAAT+TGG	0.418933	5.4:-52266625	None:intergenic
ATAATGGGTGAAGAATTATC+AGG	0.435228	5.4:+52266569	None:intergenic
CCTTCAGGAACTACAAAACT+AGG	0.437724	5.4:+52267229	MS.gene061528:CDS
ACAGGGTTTGGAGAACCAAT+TGG	0.438427	5.4:+52266610	MS.gene061528:CDS
GCCACAGCAACAACACTACA+AGG	0.474569	5.4:+52267204	MS.gene061528:CDS
CAATTGGAAATCAGCCTTCG+TGG	0.498114	5.4:+52266626	MS.gene061528:CDS
GCCTTGTAGTGTTGTTGCTG+TGG	0.521291	5.4:-52267205	None:intergenic
TATGGAACAAAAGATAAGAA+TGG	0.521790	5.4:+52267100	MS.gene061528:CDS
TGGCTGAGCTGGAGATTCAC+AGG	0.536518	5.4:-52267181	None:intergenic
TTGAGTCTCACAAATGGTGT+AGG	0.558166	5.4:+52267136	MS.gene061528:CDS
AAATGTCGAACTGTATCGTA+AGG	0.559396	5.4:+52266919	MS.gene061528:CDS
AATACAAGAACTAAATCGAA+AGG	0.627509	5.4:+52266806	MS.gene061528:CDS
CTTCAGGAACTACAAAACTA+GGG	0.628417	5.4:+52267230	MS.gene061528:CDS
TTGACAGTCAAAGAATTACA+GGG	0.630718	5.4:+52266593	MS.gene061528:CDS
CTCACAAATGGTGTAGGCAT+AGG	0.634440	5.4:+52267142	MS.gene061528:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAAAAAAAAAAAAAAACTA+TGG	-	chr5.4:52266718-52266737	None:intergenic	10.0%
!!!	AATTATCATTTTTTTTAATG+AGG	+	chr5.4:52267027-52267046	MS.gene061528:intron	10.0%
!!	AAATATGAAATTGAAAAAGA+AGG	+	chr5.4:52266961-52266980	MS.gene061528:CDS	15.0%
!!	TTTCTTAATTTATCCATTTG+AGG	-	chr5.4:52266837-52266856	None:intergenic	20.0%
!!!	AATATGTTTTTATATTCTGC+AGG	+	chr5.4:52266877-52266896	MS.gene061528:intron	20.0%
!!!	ATATGTTTTTATATTCTGCA+GGG	+	chr5.4:52266878-52266897	MS.gene061528:intron	20.0%
!!!	TCATATTTTCTTGACAAATT+AGG	-	chr5.4:52266949-52266968	None:intergenic	20.0%
!	AATACAAGAACTAAATCGAA+AGG	+	chr5.4:52266806-52266825	MS.gene061528:CDS	25.0%
!	TATGGAACAAAAGATAAGAA+TGG	+	chr5.4:52267100-52267119	MS.gene061528:CDS	25.0%
!!	GAAATTGAAAAAGAAGGTAT+TGG	+	chr5.4:52266967-52266986	MS.gene061528:intron	25.0%
!!	TAGTTTTTGATCACGATTAA+TGG	-	chr5.4:52267283-52267302	None:intergenic	25.0%
	AGTCAATTGTATGTCAACTA+TGG	-	chr5.4:52266759-52266778	None:intergenic	30.0%
	TTGACAGTCAAAGAATTACA+GGG	+	chr5.4:52266593-52266612	MS.gene061528:CDS	30.0%
	TTTGACAGTCAAAGAATTAC+AGG	+	chr5.4:52266592-52266611	MS.gene061528:CDS	30.0%
!	AAAGAGATTTTCACTAACTC+AGG	+	chr5.4:52267075-52267094	MS.gene061528:intron	30.0%
!	GAGTTAGTGAAAATCTCTTT+TGG	-	chr5.4:52267075-52267094	None:intergenic	30.0%
!!	AAAGAAGGTATTGGTTTGTA+TGG	+	chr5.4:52266976-52266995	MS.gene061528:intron	30.0%
	AAATGTCGAACTGTATCGTA+AGG	+	chr5.4:52266919-52266938	MS.gene061528:CDS	35.0%
	AGTCAAAGAATTACAGGGTT+TGG	+	chr5.4:52266598-52266617	MS.gene061528:CDS	35.0%
	CTTCAGGAACTACAAAACTA+GGG	+	chr5.4:52267230-52267249	MS.gene061528:CDS	35.0%
	TTGGAGTTCAGAATATAGCA+AGG	-	chr5.4:52267056-52267075	None:intergenic	35.0%
!	GACATACAATTGACTTATGC+AGG	+	chr5.4:52266764-52266783	MS.gene061528:intron	35.0%
!	GATACAGTTCGACATTTTCT+TGG	-	chr5.4:52266916-52266935	None:intergenic	35.0%
!	TATGCAGGAGCAACTTTTTA+TGG	+	chr5.4:52266779-52266798	MS.gene061528:intron	35.0%
!	TTTTCACTAACTCAGGTCTA+TGG	+	chr5.4:52267082-52267101	MS.gene061528:intron	35.0%
!!!	AGAGTTTTGAGTCTCACAAA+TGG	+	chr5.4:52267130-52267149	MS.gene061528:CDS	35.0%
!!!	CTAGTTTTGTAGTTCCTGAA+GGG	-	chr5.4:52267231-52267250	None:intergenic	35.0%
!!!	TAGTTTTGTAGTTCCTGAAG+GGG	-	chr5.4:52267230-52267249	None:intergenic	35.0%
	CCTTCAGGAACTACAAAACT+AGG	+	chr5.4:52267229-52267248	MS.gene061528:CDS	40.0%
	TCGTGGTGTTCGTATGAAAA+AGG	+	chr5.4:52266643-52266662	MS.gene061528:CDS	40.0%
	TGGAGATTCACAGGTACATT+TGG	-	chr5.4:52267175-52267194	None:intergenic	40.0%
	TTGAGTCTCACAAATGGTGT+AGG	+	chr5.4:52267136-52267155	MS.gene061528:CDS	40.0%
!	TTTTCATACGAACACCACGA+AGG	-	chr5.4:52266643-52266662	None:intergenic	40.0%
!!!	CCTAGTTTTGTAGTTCCTGA+AGG	-	chr5.4:52267232-52267251	None:intergenic	40.0%
	ACAGGGTTTGGAGAACCAAT+TGG	+	chr5.4:52266610-52266629	MS.gene061528:CDS	45.0%
	CAATTGGAAATCAGCCTTCG+TGG	+	chr5.4:52266626-52266645	MS.gene061528:CDS	45.0%
	CTCACAAATGGTGTAGGCAT+AGG	+	chr5.4:52267142-52267161	MS.gene061528:CDS	45.0%
	TCGAAAGGTCAGACCTCAAA+TGG	+	chr5.4:52266821-52266840	MS.gene061528:intron	45.0%
!	CACGAAGGCTGATTTCCAAT+TGG	-	chr5.4:52266628-52266647	None:intergenic	45.0%
	GCCACAGCAACAACACTACA+AGG	+	chr5.4:52267204-52267223	MS.gene061528:CDS	50.0%
!	GCCTTGTAGTGTTGTTGCTG+TGG	-	chr5.4:52267208-52267227	None:intergenic	50.0%
!	TGTAGTGTTGTTGCTGTGGC+TGG	-	chr5.4:52267204-52267223	None:intergenic	50.0%
	CAACACTACAAGGCCCCTTC+AGG	+	chr5.4:52267214-52267233	MS.gene061528:CDS	55.0%
	TGGCTGAGCTGGAGATTCAC+AGG	-	chr5.4:52267184-52267203	None:intergenic	55.0%
!	GTTGCTGTGGCTGGCTGAGC+TGG	-	chr5.4:52267195-52267214	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	52266572	52267306	52266572	ID=MS.gene061528
chr5.4	mRNA	52266572	52267306	52266572	ID=MS.gene061528.t1;Parent=MS.gene061528
chr5.4	exon	52266572	52266664	52266572	ID=MS.gene061528.t1.exon1;Parent=MS.gene061528.t1
chr5.4	CDS	52266572	52266664	52266572	ID=cds.MS.gene061528.t1;Parent=MS.gene061528.t1
chr5.4	exon	52266786	52266827	52266786	ID=MS.gene061528.t1.exon2;Parent=MS.gene061528.t1
chr5.4	CDS	52266786	52266827	52266786	ID=cds.MS.gene061528.t1;Parent=MS.gene061528.t1
chr5.4	exon	52266899	52266982	52266899	ID=MS.gene061528.t1.exon3;Parent=MS.gene061528.t1
chr5.4	CDS	52266899	52266982	52266899	ID=cds.MS.gene061528.t1;Parent=MS.gene061528.t1
chr5.4	exon	52267097	52267306	52267097	ID=MS.gene061528.t1.exon4;Parent=MS.gene061528.t1
chr5.4	CDS	52267097	52267306	52267097	ID=cds.MS.gene061528.t1;Parent=MS.gene061528.t1

Gene Sequence

>MS.gene061528

ATGGGTGAAGAATTATCAGGTTTGACAGTCAAAGAATTACAGGGTTTGGAGAACCAATTGGAAATCAGCCTTCGTGGTGTTCGTATGAAAAAGGTTTGCATTCTCTATCAAAAATACAGTTCTCAAGTTTGCATCTAAATATACCATAGTTTTTTTTTTTTTTTTGACAATGCATCTAAAGATACCATAGTTGACATACAATTGACTTATGCAGGAGCAACTTTTTATGGATGAAATACAAGAACTAAATCGAAAGGTCAGACCTCAAATGGATAAATTAAGAAACTCAGCTATTCGTTAATTAAAATATGTTTTTATATTCTGCAGGGAGACATCATAAACCAAGAAAATGTCGAACTGTATCGTAAGGTAAACCTAATTTGTCAAGAAAATATGAAATTGAAAAAGAAGGTATTGGTTTGTATGGAAATAGAAACTAAAATAATCTCAAACTAAATTATCATTTTTTTTAATGAGGCAACCTTGCTATATTCTGAACTCCAAAAGAGATTTTCACTAACTCAGGTCTATGGAACAAAAGATAAGAATGGAACAAACAGAGTTTTGAGTCTCACAAATGGTGTAGGCATAGGAGACGATCCAAATGTACCTGTGAATCTCCAGCTCAGCCAGCCACAGCAACAACACTACAAGGCCCCTTCAGGAACTACAAAACTAGGGTATGATTTTGATCATTTAGCTAGTATACCATTAATCGTGATCAAAAACTATTGA

Protein sequence

>MS.gene061528.t1

MGEELSGLTVKELQGLENQLEISLRGVRMKKEQLFMDEIQELNRKGDIINQENVELYRKVNLICQENMKLKKKVYGTKDKNGTNRVLSLTNGVGIGDDPNVPVNLQLSQPQQQHYKAPSGTTKLGYDFDHLASIPLIVIKNY