AlfalfaGEDB | MS.gene061705

M. sativa cultivar XinJiangDaYe / MS.gene061705

KEGG: K12836
GO: GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0016070 GO:0016071 GO:0032991 GO:0034641 GO:0043167 GO:0043169 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0046872 GO:0071704 GO:0089701 GO:0090304 GO:0097159 GO:1901360 GO:1901363
PFAM: PF00642.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061705.t1	XP_024634157.1	100	189	0	0	1	189	1	189	7.60E-109	403.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061705.t1	Q9FMY5	84.7	189	29	0	1	189	1	189	5.9e-94	345.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061705.t1	G7IA30	100.0	189	0	0	1	189	1	189	5.4e-109	403.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene061705	MS.gene20758	PPI
MS.gene061705	MS.gene025725	PPI
MS.gene43805	MS.gene061705	PPI
MS.gene061705	MS.gene70370	PPI
MS.gene061705	MS.gene88797	PPI
MS.gene061705	MS.gene07236	PPI
MS.gene061705	MS.gene36138	PPI
MS.gene061705	MS.gene025726	PPI
MS.gene061705	MS.gene005567	PPI
MS.gene061705	MS.gene028438	PPI
MS.gene061705	MS.gene44906	PPI
MS.gene35022	MS.gene061705	PPI
MS.gene061705	MS.gene22969	PPI
MS.gene061705	MS.gene06988	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061705.t1	MTR_1g035130	100.000	228	0	0	1	228	1	228	1.35e-170	471
MS.gene061705.t1	MTR_2g084715	81.183	186	35	0	1	186	1	186	1.34e-118	340
MS.gene061705.t1	MTR_7g103630	64.257	249	59	4	1	228	1	240	4.59e-110	319
MS.gene061705.t1	MTR_2g005450	40.719	167	94	2	9	172	184	348	5.48e-37	137

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061705.t1	AT5G42820	78.723	235	43	1	1	228	1	235	4.50e-127	360
MS.gene061705.t1	AT5G42820	78.723	235	43	1	1	228	1	235	4.50e-127	360
MS.gene061705.t1	AT1G27650	72.541	244	51	3	1	228	1	244	1.64e-118	339
MS.gene061705.t1	AT1G27650	81.481	135	25	0	51	185	1	135	1.10e-77	234
MS.gene061705.t1	AT1G10320	38.922	167	97	2	9	172	237	401	1.34e-36	136
MS.gene061705.t1	AT1G10320	38.922	167	97	2	9	172	228	392	1.44e-36	136
MS.gene061705.t1	AT3G44785	81.481	54	10	0	1	54	1	54	2.69e-28	102

Find 60 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGAATGTCTGTGATAATT+TGG	0.270895	1.3:-19314258	MS.gene061705:CDS
TTCACCGAGCTTTCGAAATT+CGG	0.280926	1.3:-19314293	MS.gene061705:CDS
TGTTGGAGAGGAGGAGAGTT+GGG	0.288016	1.3:+19314420	None:intergenic
GAGGAGAGTTGGGGAGATTG+TGG	0.325267	1.3:+19314430	None:intergenic
TTTATGCATGTTAAGAAGAT+TGG	0.350465	1.3:-19314041	MS.gene061705:CDS
TGACCGTTTGGATCGACGCC+GGG	0.392644	1.3:+19314371	None:intergenic
AGAAGATTGGTAGGGAGTTG+AGG	0.400429	1.3:-19314028	MS.gene061705:CDS
GGGAGATTGTGGGACGGTTG+TGG	0.404937	1.3:+19314441	None:intergenic
AGGAGAGTTGGGGAGATTGT+GGG	0.405421	1.3:+19314431	None:intergenic
GCCTCACGAAAATCCGTAAC+AGG	0.411096	1.3:+19314116	None:intergenic
AGAAATCTTCGAAGTGTTGT+TGG	0.412558	1.3:+19314327	None:intergenic
GGATCGATTGGTTGACCGTT+TGG	0.421209	1.3:+19314359	None:intergenic
CTTCGCAGCGGAGCAGGAGC+CGG	0.428582	1.3:-19313992	MS.gene061705:CDS
GAGGAGCAGAAGCAGAGAAC+GGG	0.437845	1.3:-19313940	MS.gene061705:CDS
AGCGGAGCAGGAGCCGGAGC+AGG	0.446956	1.3:-19313986	MS.gene061705:CDS
ATGTTGGAGAGGAGGAGAGT+TGG	0.454313	1.3:+19314419	None:intergenic
AATTTGGCTGATCATATGAT+TGG	0.466245	1.3:-19314242	MS.gene061705:CDS
AAGACGAAGTAGTGATAGGT+AGG	0.469341	1.3:-19313883	None:intergenic
CTGCTCCTCCTTCTCCTCCT+CGG	0.469594	1.3:+19313954	None:intergenic
GCGCCGATCTTGAAGTAGAA+AGG	0.491890	1.3:+19314494	None:intergenic
GTAATAGTCCGAGGAGGAGA+AGG	0.500049	1.3:-19313962	MS.gene061705:CDS
TGGATCTGACGCGGATCGAT+TGG	0.504819	1.3:+19314347	None:intergenic
CGCCGATCTTGAAGTAGAAA+GGG	0.506625	1.3:+19314495	None:intergenic
TCGGACTATTACTCCTGCTC+CGG	0.509383	1.3:+19313973	None:intergenic
GTGGAAGACGAAGTAGTGAT+AGG	0.511952	1.3:-19313887	MS.gene061705:CDS
TGCCCTTTCTACTTCAAGAT+CGG	0.519944	1.3:-19314497	MS.gene061705:CDS
GGAGGAGCAGAAGCAGAGAA+CGG	0.520575	1.3:-19313941	MS.gene061705:CDS
GTTGGAGAGGAGGAGAGTTG+GGG	0.525720	1.3:+19314421	None:intergenic
GGCGACTTGTCGGCAGTATG+AGG	0.530954	1.3:-19314096	MS.gene061705:CDS
GCGGCGTTAGCGAGTTTGAG+AGG	0.538412	1.3:-19314179	MS.gene061705:CDS
TGCATGTTAAGAAGATTGGT+AGG	0.545724	1.3:-19314037	MS.gene061705:CDS
GAACACTTAGCATCAATCTT+CGG	0.547030	1.3:-19314542	MS.gene061705:CDS
GACGAAGTAGTGATAGGTAG+GGG	0.558432	1.3:-19313881	None:intergenic
AAGATCGGCGCGTGTCGTCA+CGG	0.559187	1.3:-19314482	MS.gene061705:CDS
TTGACCGTTTGGATCGACGC+CGG	0.564773	1.3:+19314370	None:intergenic
AGACGAAGTAGTGATAGGTA+GGG	0.566122	1.3:-19313882	None:intergenic
AGATTGGTAGGGAGTTGAGG+AGG	0.577482	1.3:-19314025	MS.gene061705:CDS
CGTTAGCGAGTTTGAGAGGG+AGG	0.578892	1.3:-19314175	MS.gene061705:CDS
CGGCGTTAGCGAGTTTGAGA+GGG	0.582628	1.3:-19314178	MS.gene061705:CDS
GCATGTTAAGAAGATTGGTA+GGG	0.590056	1.3:-19314036	MS.gene061705:CDS
GACCGTTTGGATCGACGCCG+GGG	0.598231	1.3:+19314372	None:intergenic
ATAGTCCGAGGAGGAGAAGG+AGG	0.599421	1.3:-19313959	MS.gene061705:CDS
CGTCCCGACATCATCACCCC+CGG	0.623033	1.3:-19314389	MS.gene061705:CDS
GACGTTGGTACATGTTGGAG+AGG	0.624648	1.3:+19314408	None:intergenic
GAGGAGAATAGCTGTAATCG+TGG	0.625598	1.3:-19314077	MS.gene061705:CDS
GTAACCGAATTTCGAAAGCT+CGG	0.636434	1.3:+19314289	None:intergenic
AGAGGAGGATCATGCTGCGG+CGG	0.637001	1.3:-19314198	MS.gene061705:CDS
ACCGTTTGGATCGACGCCGG+GGG	0.637319	1.3:+19314373	None:intergenic
GTTGGTACATGTTGGAGAGG+AGG	0.641509	1.3:+19314411	None:intergenic
GTCGGGACGTTGGTACATGT+TGG	0.644753	1.3:+19314403	None:intergenic
AAGTGTTGTTGGATCTGACG+CGG	0.652115	1.3:+19314338	None:intergenic
GATAGAGATTATGATTCGCG+TGG	0.653513	1.3:-19313906	MS.gene061705:CDS
GGTTGTGGAGACGTGAGCAA+CGG	0.653827	1.3:+19314456	None:intergenic
GGGTGATGATGTCGGGACGT+TGG	0.655152	1.3:+19314393	None:intergenic
GGAGCAGGAGTAATAGTCCG+AGG	0.660385	1.3:-19313971	MS.gene061705:CDS
TAAAGAGGAGGATCATGCTG+CGG	0.665212	1.3:-19314201	MS.gene061705:CDS
GAGTTGGGGAGATTGTGGGA+CGG	0.682417	1.3:+19314435	None:intergenic
GAGAATAGCTGTAATCGTGG+TGG	0.695229	1.3:-19314074	MS.gene061705:CDS
GCAGGAGTAATAGTCCGAGG+AGG	0.699313	1.3:-19313968	MS.gene061705:CDS
GAATCATAATCTCTATCACG+CGG	0.759540	1.3:+19313912	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTTATGCATGTTAAGAAGAT+TGG	-	chr1.3:19314391-19314410	MS.gene061705:CDS	25.0%
!!!	TGTTTATGTGCTTTTTAAAG+AGG	-	chr1.3:19314216-19314235	MS.gene061705:CDS	25.0%
	AATTTGGCTGATCATATGAT+TGG	-	chr1.3:19314190-19314209	MS.gene061705:CDS	30.0%
	GTTGAATGTCTGTGATAATT+TGG	-	chr1.3:19314174-19314193	MS.gene061705:CDS	30.0%
!!	TTATGTGCTTTTTAAAGAGG+AGG	-	chr1.3:19314219-19314238	MS.gene061705:CDS	30.0%
	GAACACTTAGCATCAATCTT+CGG	-	chr1.3:19313890-19313909	MS.gene061705:CDS	35.0%
	GAATCATAATCTCTATCACG+CGG	+	chr1.3:19314523-19314542	None:intergenic	35.0%
	GCATGTTAAGAAGATTGGTA+GGG	-	chr1.3:19314396-19314415	MS.gene061705:CDS	35.0%
	GGAGAAAAATCCGCTAAAAT+CGG	+	chr1.3:19314298-19314317	None:intergenic	35.0%
	TGCATGTTAAGAAGATTGGT+AGG	-	chr1.3:19314395-19314414	MS.gene061705:CDS	35.0%
!!	AGAAATCTTCGAAGTGTTGT+TGG	+	chr1.3:19314108-19314127	None:intergenic	35.0%
	GATAGAGATTATGATTCGCG+TGG	-	chr1.3:19314526-19314545	MS.gene061705:CDS	40.0%
	GTAACCGAATTTCGAAAGCT+CGG	+	chr1.3:19314146-19314165	None:intergenic	40.0%
	TGCCCTTTCTACTTCAAGAT+CGG	-	chr1.3:19313935-19313954	MS.gene061705:CDS	40.0%
	TTCACCGAGCTTTCGAAATT+CGG	-	chr1.3:19314139-19314158	MS.gene061705:CDS	40.0%
!	GGAAATTGTTTTCTTCGCAG+CGG	-	chr1.3:19314428-19314447	MS.gene061705:CDS	40.0%
!!	AGCGGATTTTTCTCCTGTTA+CGG	-	chr1.3:19314303-19314322	MS.gene061705:CDS	40.0%
!!	TGAGAGGGAGGTTTTATGAA+GGG	-	chr1.3:19314269-19314288	MS.gene061705:CDS	40.0%
!!	TTGAGAGGGAGGTTTTATGA+AGG	-	chr1.3:19314268-19314287	MS.gene061705:CDS	40.0%
	AGAAGATTGGTAGGGAGTTG+AGG	-	chr1.3:19314404-19314423	MS.gene061705:CDS	45.0%
	CGCCGATCTTGAAGTAGAAA+GGG	+	chr1.3:19313940-19313959	None:intergenic	45.0%
	GAGAATAGCTGTAATCGTGG+TGG	-	chr1.3:19314358-19314377	MS.gene061705:CDS	45.0%
	GAGGAGAATAGCTGTAATCG+TGG	-	chr1.3:19314355-19314374	MS.gene061705:CDS	45.0%
	GTGGAAGACGAAGTAGTGAT+AGG	-	chr1.3:19314545-19314564	MS.gene061705:CDS	45.0%
	TAAAGAGGAGGATCATGCTG+CGG	-	chr1.3:19314231-19314250	MS.gene061705:CDS	45.0%
!	AAGTGTTGTTGGATCTGACG+CGG	+	chr1.3:19314097-19314116	None:intergenic	45.0%
!	ATTTTCGTGAGGCGACTTGT+CGG	-	chr1.3:19314326-19314345	MS.gene061705:CDS	45.0%
!	TCCTGTTACGGATTTTCGTG+AGG	-	chr1.3:19314315-19314334	MS.gene061705:CDS	45.0%
	AGATTGGTAGGGAGTTGAGG+AGG	-	chr1.3:19314407-19314426	MS.gene061705:CDS	50.0%
	AGGAGAGTTGGGGAGATTGT+GGG	+	chr1.3:19314004-19314023	None:intergenic	50.0%
	ATGTTGGAGAGGAGGAGAGT+TGG	+	chr1.3:19314016-19314035	None:intergenic	50.0%
	GACGTTGGTACATGTTGGAG+AGG	+	chr1.3:19314027-19314046	None:intergenic	50.0%
	GCCTCACGAAAATCCGTAAC+AGG	+	chr1.3:19314319-19314338	None:intergenic	50.0%
	GTAATAGTCCGAGGAGGAGA+AGG	-	chr1.3:19314470-19314489	MS.gene061705:CDS	50.0%
	GTTGGTACATGTTGGAGAGG+AGG	+	chr1.3:19314024-19314043	None:intergenic	50.0%
	TCGGACTATTACTCCTGCTC+CGG	+	chr1.3:19314462-19314481	None:intergenic	50.0%
	TGTTGGAGAGGAGGAGAGTT+GGG	+	chr1.3:19314015-19314034	None:intergenic	50.0%
!	GCGCCGATCTTGAAGTAGAA+AGG	+	chr1.3:19313941-19313960	None:intergenic	50.0%
!	GGATCGATTGGTTGACCGTT+TGG	+	chr1.3:19314076-19314095	None:intergenic	50.0%
!!	GAGGGAGGTTTTATGAAGGG+AGG	-	chr1.3:19314272-19314291	MS.gene061705:CDS	50.0%
!!	TGAAGGGAGGCCGATTTTAG+CGG	-	chr1.3:19314285-19314304	MS.gene061705:CDS	50.0%
	ATAGTCCGAGGAGGAGAAGG+AGG	-	chr1.3:19314473-19314492	MS.gene061705:CDS	55.0%
	CGGCGTTAGCGAGTTTGAGA+GGG	-	chr1.3:19314254-19314273	MS.gene061705:CDS	55.0%
	CGTTAGCGAGTTTGAGAGGG+AGG	-	chr1.3:19314257-19314276	MS.gene061705:CDS	55.0%
	GAGGAGAGTTGGGGAGATTG+TGG	+	chr1.3:19314005-19314024	None:intergenic	55.0%
	GAGGAGCAGAAGCAGAGAAC+GGG	-	chr1.3:19314492-19314511	MS.gene061705:CDS	55.0%
	GAGTTGGGGAGATTGTGGGA+CGG	+	chr1.3:19314000-19314019	None:intergenic	55.0%
	GCAGGAGTAATAGTCCGAGG+AGG	-	chr1.3:19314464-19314483	MS.gene061705:CDS	55.0%
	GGAGCAGGAGTAATAGTCCG+AGG	-	chr1.3:19314461-19314480	MS.gene061705:CDS	55.0%
	GGAGGAGCAGAAGCAGAGAA+CGG	-	chr1.3:19314491-19314510	MS.gene061705:CDS	55.0%
	GGTTGTGGAGACGTGAGCAA+CGG	+	chr1.3:19313979-19313998	None:intergenic	55.0%
	GTCGGGACGTTGGTACATGT+TGG	+	chr1.3:19314032-19314051	None:intergenic	55.0%
	GTTGGAGAGGAGGAGAGTTG+GGG	+	chr1.3:19314014-19314033	None:intergenic	55.0%
	TGGATCTGACGCGGATCGAT+TGG	+	chr1.3:19314088-19314107	None:intergenic	55.0%
	TTGACCGTTTGGATCGACGC+CGG	+	chr1.3:19314065-19314084	None:intergenic	55.0%
!	TGTTTTCTTCGCAGCGGAGC+AGG	-	chr1.3:19314434-19314453	MS.gene061705:CDS	55.0%
	AAGATCGGCGCGTGTCGTCA+CGG	-	chr1.3:19313950-19313969	MS.gene061705:CDS	60.0%
	AGAGGAGGATCATGCTGCGG+CGG	-	chr1.3:19314234-19314253	MS.gene061705:CDS	60.0%
	CTGCTCCTCCTTCTCCTCCT+CGG	+	chr1.3:19314481-19314500	None:intergenic	60.0%
	GCGGCGTTAGCGAGTTTGAG+AGG	-	chr1.3:19314253-19314272	MS.gene061705:CDS	60.0%
	GGCGACTTGTCGGCAGTATG+AGG	-	chr1.3:19314336-19314355	MS.gene061705:CDS	60.0%
	GGGAGATTGTGGGACGGTTG+TGG	+	chr1.3:19313994-19314013	None:intergenic	60.0%
	TGACCGTTTGGATCGACGCC+GGG	+	chr1.3:19314064-19314083	None:intergenic	60.0%
!	GGGTGATGATGTCGGGACGT+TGG	+	chr1.3:19314042-19314061	None:intergenic	60.0%
	ACCGTTTGGATCGACGCCGG+GGG	+	chr1.3:19314062-19314081	None:intergenic	65.0%
	CGTCCCGACATCATCACCCC+CGG	-	chr1.3:19314043-19314062	MS.gene061705:CDS	65.0%
	GACCGTTTGGATCGACGCCG+GGG	+	chr1.3:19314063-19314082	None:intergenic	65.0%
!	ACGCCGGGGGTGATGATGTC+GGG	+	chr1.3:19314049-19314068	None:intergenic	65.0%
!	GACGCCGGGGGTGATGATGT+CGG	+	chr1.3:19314050-19314069	None:intergenic	65.0%
!!	ACCCCCGGCGTCGATCCAAA+CGG	-	chr1.3:19314058-19314077	MS.gene061705:CDS	65.0%
	CTTCGCAGCGGAGCAGGAGC+CGG	-	chr1.3:19314440-19314459	MS.gene061705:CDS	70.0%
	AGCGGAGCAGGAGCCGGAGC+AGG	-	chr1.3:19314446-19314465	MS.gene061705:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	19313884	19314570	19313884	ID=MS.gene061705
chr1.3	mRNA	19313884	19314570	19313884	ID=MS.gene061705.t1;Parent=MS.gene061705
chr1.3	exon	19313884	19314570	19313884	ID=MS.gene061705.t1.exon1;Parent=MS.gene061705.t1
chr1.3	CDS	19313884	19314570	19313884	ID=cds.MS.gene061705.t1;Parent=MS.gene061705.t1

Gene Sequence

>MS.gene061705

ATGGCGGAACACTTAGCATCAATCTTCGGCACAGAAAAAGACAGAGTAAACTGCCCTTTCTACTTCAAGATCGGCGCGTGTCGTCACGGCGACCGTTGCTCACGTCTCCACAACCGTCCCACAATCTCCCCAACTCTCCTCCTCTCCAACATGTACCAACGTCCCGACATCATCACCCCCGGCGTCGATCCAAACGGTCAACCAATCGATCCGCGTCAGATCCAACAACACTTCGAAGATTTCTACGAAGATATCTTCACCGAGCTTTCGAAATTCGGTTACGTTGAAACGTTGAATGTCTGTGATAATTTGGCTGATCATATGATTGGTAATGTTTATGTGCTTTTTAAAGAGGAGGATCATGCTGCGGCGGCGTTAGCGAGTTTGAGAGGGAGGTTTTATGAAGGGAGGCCGATTTTAGCGGATTTTTCTCCTGTTACGGATTTTCGTGAGGCGACTTGTCGGCAGTATGAGGAGAATAGCTGTAATCGTGGTGGTTATTGTAATTTTATGCATGTTAAGAAGATTGGTAGGGAGTTGAGGAGGAAATTGTTTTCTTCGCAGCGGAGCAGGAGCCGGAGCAGGAGTAATAGTCCGAGGAGGAGAAGGAGGAGCAGAAGCAGAGAACGGGAGAGACCGCGTGATAGAGATTATGATTCGCGTGGAAGACGAAGTAGTGATAGGTAG

Protein sequence

>MS.gene061705.t1

MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDIITPGVDPNGQPIDPRQIQQHFEDFYEDIFTELSKFGYVETLNVCDNLADHMIGNVYVLFKEEDHAAAALASLRGRFYEGRPILADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKKIGRELRRKLFSSQRSRSRSRSNSPRRRRRSRSRERERPRDRDYDSRGRRSSDR