AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062065

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062065.t1 MTR_2g045360 96.209 422 15 1 1 421 8 429 0.0 800
MS.gene062065.t1 MTR_3g053330 47.598 229 107 6 178 402 225 444 8.25e-58 196
MS.gene062065.t1 MTR_7g110720 34.711 363 183 12 57 408 92 411 8.26e-51 177
MS.gene062065.t1 MTR_7g110720 40.370 270 132 9 150 408 133 384 5.15e-50 174
MS.gene062065.t1 MTR_2g088000 35.941 409 175 16 59 401 167 554 6.14e-49 176
MS.gene062065.t1 MTR_4g132430 37.050 278 160 4 61 331 114 383 8.25e-49 173
MS.gene062065.t1 MTR_4g132430 38.132 257 144 4 82 331 1 249 5.64e-47 165
MS.gene062065.t1 MTR_2g023930 33.663 404 193 13 61 404 143 531 1.22e-44 163
MS.gene062065.t1 MTR_4g049790 34.940 332 165 7 61 350 131 453 3.56e-43 159
MS.gene062065.t1 MTR_4g049760 54.430 158 65 3 175 331 261 412 2.09e-40 151
MS.gene062065.t1 MTR_4g094412 36.822 258 121 7 188 408 227 479 2.18e-37 142
MS.gene062065.t1 MTR_4g094412 36.822 258 121 7 188 408 218 470 2.21e-37 142
MS.gene062065.t1 MTR_4g107970 63.158 95 31 1 174 264 144 238 4.52e-37 142
MS.gene062065.t1 MTR_4g107970 63.158 95 31 1 174 264 221 315 9.76e-37 142
MS.gene062065.t1 MTR_7g009730 61.386 101 38 1 178 277 192 292 2.62e-36 140
MS.gene062065.t1 MTR_4g047580 37.165 261 137 8 159 401 239 490 2.90e-33 131
MS.gene062065.t1 MTR_4g007060 32.110 218 97 8 185 399 107 276 2.20e-24 102
MS.gene062065.t1 MTR_4g007060 32.110 218 97 8 185 399 141 310 3.50e-24 102
MS.gene062065.t1 MTR_5g074400 49.020 102 50 2 165 266 382 481 1.70e-23 103
MS.gene062065.t1 MTR_5g074400 46.667 60 30 2 205 264 244 301 8.39e-11 64.3
MS.gene062065.t1 MTR_2g105060 39.850 133 70 3 187 314 72 199 5.35e-23 97.4
MS.gene062065.t1 MTR_2g105060 39.850 133 70 3 187 314 147 274 1.17e-22 98.2
MS.gene062065.t1 MTR_7g109800 37.671 146 80 4 188 323 129 273 1.94e-22 97.1
MS.gene062065.t1 MTR_3g106060 50.602 83 38 1 202 284 136 215 3.17e-22 96.3
MS.gene062065.t1 MTR_1g013790 48.235 85 42 2 202 284 99 183 3.29e-22 95.5
MS.gene062065.t1 MTR_1g013760 50.685 73 36 0 189 261 125 197 5.51e-22 95.5
MS.gene062065.t1 MTR_5g094430 45.556 90 48 1 188 277 214 302 2.29e-21 95.9
MS.gene062065.t1 MTR_5g094430 45.556 90 48 1 188 277 236 324 2.32e-21 95.9
MS.gene062065.t1 MTR_3g009470 37.584 149 81 5 154 292 435 581 3.59e-21 96.7
MS.gene062065.t1 MTR_3g009470 39.362 94 54 3 176 268 251 342 1.05e-13 73.6
MS.gene062065.t1 MTR_5g094430 50.633 79 38 1 188 266 348 425 4.06e-21 95.9
MS.gene062065.t1 MTR_7g079010 35.088 171 85 6 109 274 58 207 6.73e-21 90.9
MS.gene062065.t1 MTR_7g100510 44.954 109 52 3 177 280 329 434 9.34e-21 94.4
MS.gene062065.t1 MTR_7g100510 42.308 78 43 2 205 282 190 265 3.65e-12 68.2
MS.gene062065.t1 MTR_3g031220 51.282 78 37 1 187 264 348 424 9.95e-21 94.7
MS.gene062065.t1 MTR_3g056100 50.633 79 38 1 188 266 242 319 1.40e-20 93.6
MS.gene062065.t1 MTR_3g056100 50.633 79 38 1 188 266 255 332 1.50e-20 93.6
MS.gene062065.t1 MTR_5g044695 65.517 58 19 1 176 232 48 105 1.56e-20 86.7
MS.gene062065.t1 MTR_3g056100 50.633 79 38 1 188 266 399 476 1.87e-20 94.0
MS.gene062065.t1 MTR_3g056100 50.633 79 38 1 188 266 386 463 1.99e-20 94.0
MS.gene062065.t1 MTR_4g013260 50.000 82 37 2 186 264 315 395 3.43e-20 92.8
MS.gene062065.t1 MTR_4g013260 44.118 68 37 1 205 272 185 251 3.39e-12 68.6
MS.gene062065.t1 MTR_7g011820 47.674 86 44 1 191 276 397 481 7.35e-20 92.0
MS.gene062065.t1 MTR_7g011820 48.333 60 29 2 205 264 229 286 1.45e-11 66.6
MS.gene062065.t1 MTR_4g082580 45.977 87 46 1 189 275 263 348 8.87e-20 90.9
MS.gene062065.t1 MTR_7g080020 48.148 81 41 1 186 266 475 554 9.16e-20 92.4
MS.gene062065.t1 MTR_7g080000 36.641 131 70 2 186 304 414 543 1.26e-19 91.7
MS.gene062065.t1 MTR_5g042920 45.161 93 47 2 191 280 307 398 1.99e-19 90.1
MS.gene062065.t1 MTR_5g042920 50.000 60 28 2 205 264 152 209 6.27e-12 67.4
MS.gene062065.t1 MTR_3g095040 51.351 74 35 1 191 264 105 177 2.11e-19 86.3
MS.gene062065.t1 MTR_4g122530 47.674 86 43 2 188 273 192 275 2.56e-19 89.7
MS.gene062065.t1 MTR_6g038890 65.517 58 19 1 176 232 159 216 2.87e-19 86.3
MS.gene062065.t1 MTR_2g024350 49.296 71 36 0 205 275 9 79 4.18e-19 86.3
MS.gene062065.t1 MTR_2g033820 45.122 82 44 1 188 269 187 267 6.17e-19 88.2
MS.gene062065.t1 MTR_5g097490 40.708 113 61 3 175 283 223 333 8.30e-19 88.2
MS.gene062065.t1 MTR_7g028415 45.263 95 47 2 171 261 56 149 8.87e-19 83.6
MS.gene062065.t1 MTR_7g028710 45.263 95 47 2 171 261 56 149 8.87e-19 83.6
MS.gene062065.t1 MTR_5g091390 34.711 121 75 2 151 268 101 220 1.38e-18 86.3
MS.gene062065.t1 MTR_1g086790 44.444 81 44 1 188 268 142 221 1.41e-18 86.7
MS.gene062065.t1 MTR_4g094588 35.252 139 70 4 191 316 165 296 2.17e-18 86.3
MS.gene062065.t1 MTR_7g071120 47.500 80 41 1 187 266 307 385 2.23e-18 87.4
MS.gene062065.t1 MTR_8g092010 47.297 74 38 1 191 264 95 167 2.96e-18 82.8
MS.gene062065.t1 MTR_2g024380 35.433 127 76 1 155 275 196 322 4.00e-18 86.7
MS.gene062065.t1 MTR_1g015140 49.315 73 36 1 189 261 87 158 5.39e-18 81.3
MS.gene062065.t1 MTR_2g016770 55.738 61 27 0 205 265 36 96 5.86e-18 82.4
MS.gene062065.t1 MTR_2g016770 55.738 61 27 0 205 265 28 88 7.02e-18 82.0
MS.gene062065.t1 MTR_4g094908 54.839 62 28 0 205 266 267 328 9.89e-18 84.3
MS.gene062065.t1 MTR_3g104750 39.560 91 54 1 171 261 71 160 1.78e-17 79.7
MS.gene062065.t1 MTR_8g096730 36.893 103 61 2 174 273 37 138 3.21e-17 78.6
MS.gene062065.t1 MTR_8g087000 56.667 60 26 0 205 264 78 137 4.30e-17 80.9
MS.gene062065.t1 MTR_8g087000 56.667 60 26 0 205 264 77 136 4.80e-17 80.5
MS.gene062065.t1 MTR_5g016610 41.837 98 48 1 175 263 161 258 6.68e-17 80.9
MS.gene062065.t1 MTR_5g043880 42.683 82 46 1 181 262 101 181 8.68e-17 80.5
MS.gene062065.t1 MTR_4g130900 44.156 77 42 1 188 264 343 418 8.81e-17 82.8
MS.gene062065.t1 MTR_1g077780 44.156 77 42 1 188 264 46 121 1.13e-16 77.0
MS.gene062065.t1 MTR_1g070455 52.459 61 29 0 205 265 294 354 1.20e-16 81.3
MS.gene062065.t1 MTR_7g109600 50.725 69 34 0 205 273 54 122 1.32e-16 79.7
MS.gene062065.t1 MTR_1g009613 52.542 59 28 0 205 263 263 321 5.85e-16 79.0
MS.gene062065.t1 MTR_2g083870 55.000 60 27 0 205 264 81 140 7.63e-16 77.4
MS.gene062065.t1 MTR_8g007270 45.882 85 41 2 181 261 127 210 1.01e-15 76.6
MS.gene062065.t1 MTR_2g032030 39.394 99 59 1 179 276 241 339 1.39e-15 79.0
MS.gene062065.t1 MTR_1g099600 43.678 87 49 0 204 290 185 271 1.43e-15 77.4
MS.gene062065.t1 MTR_3g095080 41.053 95 53 2 188 282 128 219 1.52e-15 75.9
MS.gene062065.t1 MTR_2g032030 39.394 99 59 1 179 276 241 339 1.79e-15 78.2
MS.gene062065.t1 MTR_3g095080 40.625 96 54 2 187 282 151 243 2.11e-15 75.9
MS.gene062065.t1 MTR_8g028565 51.429 70 34 0 205 274 156 225 2.68e-15 77.0
MS.gene062065.t1 MTR_8g098945 42.353 85 47 1 203 287 128 210 8.91e-15 74.7
MS.gene062065.t1 MTR_7g079040 50.000 70 35 0 205 274 155 224 1.55e-14 74.7
MS.gene062065.t1 MTR_0117s0040 49.153 59 30 0 204 262 74 132 1.62e-14 73.2
MS.gene062065.t1 MTR_4g127040 52.632 57 27 0 205 261 246 302 2.32e-14 73.9
MS.gene062065.t1 MTR_8g092140 48.438 64 32 1 198 261 154 216 9.10e-14 70.9
MS.gene062065.t1 MTR_3g009470 39.362 94 54 3 176 268 251 342 1.10e-13 73.2
MS.gene062065.t1 MTR_1g078260 28.272 191 127 5 72 261 123 304 1.28e-13 72.8
MS.gene062065.t1 MTR_3g108960 39.744 78 47 0 188 265 298 375 1.32e-13 72.4
MS.gene062065.t1 MTR_6g086555 52.308 65 29 2 205 268 207 270 5.25e-13 70.5
MS.gene062065.t1 MTR_4g007060 44.444 63 35 0 179 241 137 199 5.44e-13 68.6
MS.gene062065.t1 MTR_7g062220 52.632 57 26 1 205 261 125 180 9.00e-13 69.3
MS.gene062065.t1 MTR_8g069935 41.667 60 35 0 205 264 332 391 3.23e-12 68.6
MS.gene062065.t1 MTR_6g053200 41.667 60 35 0 205 264 331 390 3.38e-12 68.6
MS.gene062065.t1 MTR_3g031220 47.059 68 29 3 205 272 199 259 4.57e-12 67.8
MS.gene062065.t1 MTR_8g067650 45.000 60 33 0 205 264 340 399 5.27e-12 67.8
MS.gene062065.t1 MTR_8g067635 45.000 60 33 0 205 264 350 409 7.92e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062065.t1 AT4G04450 37.942 311 143 7 63 331 20 322 7.07e-53 182
MS.gene062065.t1 AT4G04450 37.942 311 143 7 63 331 114 416 4.71e-52 182
MS.gene062065.t1 AT1G62300 35.697 409 166 17 67 404 158 540 5.87e-49 175
MS.gene062065.t1 AT4G01720 33.784 370 194 9 59 399 96 443 8.62e-45 162
MS.gene062065.t1 AT4G22070 35.494 324 153 9 59 334 108 423 2.91e-43 159
MS.gene062065.t1 AT4G22070 35.494 324 153 9 59 334 108 423 2.91e-43 159
MS.gene062065.t1 AT4G22070 35.494 324 153 9 59 334 108 423 2.91e-43 159
MS.gene062065.t1 AT4G22070 35.494 324 153 9 59 334 108 423 2.91e-43 159
MS.gene062065.t1 AT5G15130 35.030 334 142 10 61 333 35 354 5.18e-42 155
MS.gene062065.t1 AT5G15130 51.534 163 63 4 180 333 157 312 1.36e-41 154
MS.gene062065.t1 AT1G68150 64.815 108 37 1 182 289 213 319 5.86e-41 149
MS.gene062065.t1 AT1G18860 33.524 349 159 10 61 336 41 389 2.01e-37 143
MS.gene062065.t1 AT1G69810 44.172 163 78 3 173 333 114 265 1.04e-32 126
MS.gene062065.t1 AT1G69810 45.513 156 72 3 180 333 179 323 1.14e-32 127
MS.gene062065.t1 AT5G07100 39.130 138 73 4 139 273 81 210 1.90e-25 103
MS.gene062065.t1 AT5G07100 39.130 138 73 4 139 273 81 210 1.90e-25 103
MS.gene062065.t1 AT5G07100 39.130 138 73 4 139 273 81 210 1.90e-25 103
MS.gene062065.t1 AT5G07100 39.130 138 73 4 139 273 81 210 1.90e-25 103
MS.gene062065.t1 AT5G07100 38.571 140 75 4 137 273 172 303 2.95e-25 105
MS.gene062065.t1 AT1G80840 31.658 199 106 6 68 264 36 206 2.05e-22 97.1
MS.gene062065.t1 AT2G38470 42.857 119 64 3 149 266 308 423 5.89e-22 98.6
MS.gene062065.t1 AT2G38470 48.333 60 29 2 205 264 185 242 5.16e-11 64.7
MS.gene062065.t1 AT4G31800 56.000 75 32 1 198 271 169 243 6.33e-22 95.9
MS.gene062065.t1 AT4G31800 56.000 75 32 1 198 271 104 178 6.35e-22 94.7
MS.gene062065.t1 AT2G47260 48.810 84 42 1 188 271 158 240 1.25e-21 95.5
MS.gene062065.t1 AT1G29860 48.148 81 41 1 188 268 120 199 1.88e-21 94.0
MS.gene062065.t1 AT5G41570 48.352 91 42 2 175 261 65 154 2.26e-21 91.3
MS.gene062065.t1 AT2G30250 47.826 92 45 2 179 268 301 391 3.50e-21 95.1
MS.gene062065.t1 AT2G04880 49.351 77 38 1 190 266 269 344 5.36e-21 95.1
MS.gene062065.t1 AT2G25000 55.556 72 31 1 197 267 95 166 5.57e-21 91.7
MS.gene062065.t1 AT2G25000 55.556 72 31 1 197 267 95 166 5.57e-21 91.7
MS.gene062065.t1 AT2G04880 49.351 77 38 1 190 266 293 368 6.72e-21 95.1
MS.gene062065.t1 AT4G18170 45.714 105 51 4 162 264 131 231 7.87e-21 92.8
MS.gene062065.t1 AT2G25000 55.556 72 31 1 197 267 117 188 9.03e-21 91.3
MS.gene062065.t1 AT2G25000 55.556 72 31 1 197 267 138 209 1.20e-20 91.7
MS.gene062065.t1 AT1G64000 51.190 84 37 2 191 274 101 180 3.03e-20 88.6
MS.gene062065.t1 AT5G56270 48.864 88 44 1 179 266 462 548 4.21e-20 93.2
MS.gene062065.t1 AT4G26640 49.351 77 38 1 188 264 189 264 4.47e-20 91.7
MS.gene062065.t1 AT4G26640 49.351 77 38 1 188 264 189 264 4.47e-20 91.7
MS.gene062065.t1 AT4G26640 49.351 77 38 1 188 264 189 264 4.47e-20 91.7
MS.gene062065.t1 AT4G26640 49.351 77 38 1 188 264 189 264 4.47e-20 91.7
MS.gene062065.t1 AT4G26640 48.718 78 39 1 187 264 324 400 7.39e-20 92.0
MS.gene062065.t1 AT4G26640 49.351 77 38 1 188 264 293 368 8.34e-20 91.7
MS.gene062065.t1 AT4G26640 49.351 77 38 1 188 264 365 440 9.64e-20 92.0
MS.gene062065.t1 AT5G26170 52.778 72 33 1 190 261 99 169 1.25e-19 86.3
MS.gene062065.t1 AT1G13960 50.000 74 36 1 191 264 243 315 1.76e-19 89.7
MS.gene062065.t1 AT1G13960 50.000 60 28 2 205 264 77 134 1.21e-11 66.2
MS.gene062065.t1 AT1G13960 50.000 74 36 1 191 264 396 468 2.49e-19 90.5
MS.gene062065.t1 AT1G13960 50.000 60 28 2 205 264 230 287 8.68e-12 67.4
MS.gene062065.t1 AT5G64810 48.276 87 41 2 176 262 85 167 3.92e-19 85.5
MS.gene062065.t1 AT3G62340 44.037 109 55 2 191 294 105 212 4.02e-19 87.4
MS.gene062065.t1 AT5G13080 48.193 83 39 2 191 273 54 132 4.40e-19 84.0
MS.gene062065.t1 AT5G46350 41.667 96 54 2 191 286 170 263 4.62e-19 88.2
MS.gene062065.t1 AT2G03340 48.684 76 38 1 191 266 402 476 4.76e-19 89.7
MS.gene062065.t1 AT2G03340 51.667 60 27 2 205 264 251 308 8.73e-13 70.5
MS.gene062065.t1 AT2G46130 45.263 95 48 2 180 274 6 96 4.78e-19 82.4
MS.gene062065.t1 AT5G49520 45.161 93 49 2 188 279 205 296 5.23e-19 89.0
MS.gene062065.t1 AT3G01970 50.000 74 36 1 190 263 51 123 1.33e-18 82.4
MS.gene062065.t1 AT3G01080 48.649 74 37 1 191 264 293 365 1.40e-18 87.8
MS.gene062065.t1 AT3G01080 48.649 74 37 1 191 264 331 403 1.52e-18 87.8
MS.gene062065.t1 AT1G69310 45.238 84 45 1 179 262 122 204 1.93e-18 85.5
MS.gene062065.t1 AT1G69310 45.238 84 45 1 179 262 122 204 1.93e-18 85.5
MS.gene062065.t1 AT1G69310 45.238 84 45 1 179 262 122 204 1.93e-18 85.5
MS.gene062065.t1 AT4G26440 34.459 148 83 5 154 288 311 457 2.89e-18 87.4
MS.gene062065.t1 AT4G30935 38.136 118 67 3 175 288 303 418 6.82e-18 85.9
MS.gene062065.t1 AT2G37260 39.695 131 59 6 150 267 211 334 1.55e-17 84.0
MS.gene062065.t1 AT2G37260 44.156 77 39 3 204 280 85 157 2.76e-13 71.2
MS.gene062065.t1 AT2G37260 39.695 131 59 6 150 267 209 332 1.68e-17 83.6
MS.gene062065.t1 AT2G37260 44.156 77 39 3 204 280 83 155 2.87e-13 71.2
MS.gene062065.t1 AT2G23320 57.627 59 25 0 205 263 241 299 2.64e-17 82.8
MS.gene062065.t1 AT2G37260 41.221 131 57 7 150 267 291 414 2.81e-17 84.0
MS.gene062065.t1 AT2G37260 44.156 77 39 3 204 280 165 237 3.88e-13 71.2
MS.gene062065.t1 AT2G30590 54.688 64 28 1 205 267 314 377 6.37e-17 82.4
MS.gene062065.t1 AT5G28650 57.895 57 24 0 205 261 263 319 6.54e-17 81.6
MS.gene062065.t1 AT3G04670 57.895 57 24 0 205 261 263 319 1.06e-16 81.3
MS.gene062065.t1 AT4G24240 55.932 59 26 0 205 263 282 340 2.25e-16 80.5
MS.gene062065.t1 AT1G55600 42.697 89 46 2 182 266 281 368 2.84e-16 81.3
MS.gene062065.t1 AT1G29280 45.882 85 38 1 195 279 65 141 3.35e-16 78.6
MS.gene062065.t1 AT4G31550 44.872 78 40 1 205 282 247 321 3.86e-16 79.3
MS.gene062065.t1 AT4G31550 44.872 78 40 1 205 282 246 320 4.04e-16 79.3
MS.gene062065.t1 AT2G44745 43.956 91 46 2 175 261 112 201 4.11e-16 77.4
MS.gene062065.t1 AT2G24570 53.448 58 27 0 205 262 244 301 1.02e-15 78.2
MS.gene062065.t1 AT1G30650 43.529 85 47 1 181 264 193 277 1.38e-15 78.6
MS.gene062065.t1 AT2G34830 43.529 85 47 1 181 264 191 275 1.56e-15 78.6
MS.gene062065.t1 AT4G01250 48.649 74 38 0 201 274 125 198 1.97e-15 77.0
MS.gene062065.t1 AT2G34830 43.529 85 47 1 181 264 200 284 2.16e-15 78.2
MS.gene062065.t1 AT4G23550 43.373 83 47 0 202 284 138 220 2.76e-15 76.6
MS.gene062065.t1 AT3G58710 55.000 60 27 0 205 264 72 131 4.13e-15 75.5
MS.gene062065.t1 AT3G58710 55.000 60 27 0 205 264 71 130 4.31e-15 75.5
MS.gene062065.t1 AT5G52830 50.000 70 35 0 205 274 166 235 1.30e-14 75.1
MS.gene062065.t1 AT2G46130 42.105 95 47 3 180 274 6 92 2.94e-14 68.9
MS.gene062065.t1 AT4G39410 40.000 85 50 1 177 261 196 279 4.58e-14 73.2
MS.gene062065.t1 AT5G45050 36.842 114 69 1 205 315 1153 1266 1.26e-13 73.6
MS.gene062065.t1 AT5G45050 36.842 114 69 1 205 315 1181 1294 1.28e-13 73.6
MS.gene062065.t1 AT5G45050 36.842 114 69 1 205 315 1042 1155 1.28e-13 73.6
MS.gene062065.t1 AT1G69310 41.667 84 44 2 179 262 122 200 1.38e-13 71.2
MS.gene062065.t1 AT5G43290 47.541 61 31 1 205 265 115 174 3.19e-12 67.0
MS.gene062065.t1 AT4G31800 62.222 45 16 1 198 241 169 213 4.07e-12 66.2
MS.gene062065.t1 AT2G21900 46.667 60 31 1 205 264 110 168 5.15e-12 65.1
MS.gene062065.t1 AT2G46400 36.000 100 62 1 183 280 83 182 3.22e-11 64.3
MS.gene062065.t1 AT2G40740 43.902 82 35 3 205 275 174 255 3.91e-11 63.9
MS.gene062065.t1 AT2G40740 43.902 82 35 3 205 275 183 264 5.02e-11 63.9

Find 97 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCATTGTTTATCATATT+TGG 0.055350 2.3:+45152220 None:intergenic
CACTACTCTTACTAGGTAAT+TGG 0.189983 2.3:+45152074 None:intergenic
ATTAATCACTTACTTGGATT+TGG 0.221328 2.3:+45155352 None:intergenic
TTAATCACTTACTTGGATTT+GGG 0.256367 2.3:+45155353 None:intergenic
GAAAACTTACAGTATTCTTA+TGG 0.285405 2.3:+45155813 None:intergenic
TCTTCAGAATCGTTTACTTT+TGG 0.317553 2.3:-45155035 MS.gene062065:CDS
AGCCTACTATCGTTGCAGTA+TGG 0.326812 2.3:-45154083 MS.gene062065:CDS
GGGGATTTCCTTGAATTTGA+TGG 0.332291 2.3:+45155373 None:intergenic
AAAATCTGATCGTGCCCTTA+TGG 0.343745 2.3:+45154289 None:intergenic
GGGTGTCAATGGAGAAAATA+TGG 0.346609 2.3:-45154139 MS.gene062065:CDS
TCTTTATTGTTATTATCATA+TGG 0.359665 2.3:+45155869 None:intergenic
TAGTTTACCTGCTTACGAAC+TGG 0.359755 2.3:+45154049 None:intergenic
GAAGTTGTGTTGTGTTATTA+AGG 0.369494 2.3:+45155397 None:intergenic
GATGATGTTAGGTTTGCTAT+TGG 0.371407 2.3:+45152508 None:intergenic
TCTTACTAGGTAATTGGCTT+TGG 0.372010 2.3:+45152080 None:intergenic
TTTGAGTTTGGTGGAGATAA+TGG 0.378048 2.3:+45152400 None:intergenic
GTAGCTATAACATTTGAGTT+TGG 0.383227 2.3:+45152388 None:intergenic
TCAACAAATCTACAACAATA+TGG 0.389536 2.3:-45152457 MS.gene062065:CDS
TTTAAGAGGTATGCTAAATC+TGG 0.393699 2.3:-45155077 MS.gene062065:CDS
ATGATTATGGCTCCCTTCAT+AGG 0.402020 2.3:+45152552 None:intergenic
CAATGAGAGAAAACTTGCTT+TGG 0.409723 2.3:-45152206 MS.gene062065:CDS
TGTTGAGTGCTGATGATGTT+AGG 0.413129 2.3:+45152497 None:intergenic
AGTTCAAACAATGAAATTGC+TGG 0.417191 2.3:-45155909 None:intergenic
CGATAGTAGGCTCGTGGACA+AGG 0.430122 2.3:+45154094 None:intergenic
GTAAAATCAAGTGTAATTGT+AGG 0.435039 2.3:+45152343 None:intergenic
TGTAATGTTTAGATGGTTGA+TGG 0.440145 2.3:-45154160 MS.gene062065:intron
GGAGAGCTAGAGTGTCCATA+AGG 0.442520 2.3:-45154304 MS.gene062065:CDS
AATTAACCACTACTCTTACT+AGG 0.445615 2.3:+45152067 None:intergenic
GGGATTTCCTTGAATTTGAT+GGG 0.451310 2.3:+45155374 None:intergenic
GCTATAACATTTGAGTTTGG+TGG 0.456982 2.3:+45152391 None:intergenic
AAATAATGGATTTGCCATAG+TGG 0.457421 2.3:-45154429 MS.gene062065:CDS
TTGAATTGTAAATAGTTACT+TGG 0.461039 2.3:+45152313 None:intergenic
CTGGTGGAAGTTGATGATTA+TGG 0.462621 2.3:+45152539 None:intergenic
TGAAATAAATAGCAAAATAG+AGG 0.463782 2.3:-45154351 MS.gene062065:CDS
AAGGAGAATGAAAATTTAAG+AGG 0.466438 2.3:-45155091 MS.gene062065:CDS
TAATGACCTTGGAATGGAAA+AGG 0.467036 2.3:+45152265 None:intergenic
CAACTTCCCCATCAAATTCA+AGG 0.467550 2.3:-45155381 MS.gene062065:CDS
AGATGGTTGATGGGTGTCAA+TGG 0.469133 2.3:-45154150 MS.gene062065:CDS
GCCTACTATCGTTGCAGTAT+GGG 0.470435 2.3:-45154082 MS.gene062065:CDS
GGAAACACCTCAAATGATTG+AGG 0.470497 2.3:+45152241 None:intergenic
TGCACCCAACCTCAGATGAT+TGG 0.470879 2.3:+45154327 None:intergenic
ACAGGTGCAAAGGTGTTTCA+AGG 0.472939 2.3:-45152596 MS.gene062065:intron
GCCATGTCCTCGTTCACAAA+TGG 0.479556 2.3:-45152124 MS.gene062065:CDS
CCCCATACTGCAACGATAGT+AGG 0.503349 2.3:+45154081 None:intergenic
GAAACACCTCAAATGATTGA+GGG 0.504461 2.3:+45152242 None:intergenic
GAAGCTTGATGAAGCAAAGA+AGG 0.508309 2.3:-45155110 MS.gene062065:CDS
AGGACCAATCATCTGAGGTT+GGG 0.511884 2.3:-45154331 MS.gene062065:CDS
AGTTTACCTGCTTACGAACT+GGG 0.512250 2.3:+45154050 None:intergenic
GAGAGCTAGAGTGTCCATAA+GGG 0.513998 2.3:-45154303 MS.gene062065:CDS
TTCATGCCCAGTTCGTAAGC+AGG 0.514284 2.3:-45154056 MS.gene062065:intron
CTTAATAAAAGAGTAGATCC+TGG 0.516100 2.3:+45155426 None:intergenic
GTGTTCATCACAACCTATGA+AGG 0.519912 2.3:-45152565 MS.gene062065:CDS
AGTATTCTTATGGAAGGAAG+AGG 0.520852 2.3:+45155823 None:intergenic
TAAAATCAAGTGTAATTGTA+GGG 0.521053 2.3:+45152344 None:intergenic
AAATAATCACAACTTGCTGC+TGG 0.523498 2.3:-45154978 MS.gene062065:intron
GAGGACCAATCATCTGAGGT+TGG 0.528239 2.3:-45154332 MS.gene062065:CDS
ATGATTGAGGGTAATGACCT+TGG 0.529952 2.3:+45152254 None:intergenic
TCGTGGACAAGGGTTGCCCT+TGG 0.531338 2.3:+45154105 None:intergenic
ATTGTAGGGCATGTTGGAGA+TGG 0.532259 2.3:+45152358 None:intergenic
AGTGTAATTGTAGGGCATGT+TGG 0.533130 2.3:+45152352 None:intergenic
AATCATCTGAGGTTGGGTGC+AGG 0.533609 2.3:-45154325 MS.gene062065:CDS
AATTGTAAATAGTTACTTGG+AGG 0.535746 2.3:+45152316 None:intergenic
TTGCTACAATCTGAAGGTGA+AGG 0.539687 2.3:+45154452 None:intergenic
GAGATAATGGTGAAGAGAAG+AGG 0.540861 2.3:+45152413 None:intergenic
TCCATTTGTGAACGAGGACA+TGG 0.553076 2.3:+45152123 None:intergenic
TCATTACCCTCAATCATTTG+AGG 0.553278 2.3:-45152248 MS.gene062065:CDS
ATTGTAAATAGTTACTTGGA+GGG 0.553408 2.3:+45152317 None:intergenic
GTAATGTTTAGATGGTTGAT+GGG 0.564516 2.3:-45154159 MS.gene062065:intron
TGTAGATTTGTTGATGAGGT+AGG 0.565582 2.3:+45152466 None:intergenic
GATTTGTTGATGAGGTAGGA+AGG 0.569280 2.3:+45152470 None:intergenic
AGAAACCAGTGTTACCTACT+CGG 0.569565 2.3:-45154490 MS.gene062065:CDS
ACTTACAGTATTCTTATGGA+AGG 0.574288 2.3:+45155817 None:intergenic
ATTGCAATTAACAGGTGCAA+AGG 0.574633 2.3:-45152606 MS.gene062065:intron
AATGACCTTGGAATGGAAAA+GGG 0.575264 2.3:+45152266 None:intergenic
TAATCACTTACTTGGATTTG+GGG 0.580090 2.3:+45155354 None:intergenic
AGAGAAAGATCCTTCTCTCA+AGG 0.582199 2.3:-45152161 MS.gene062065:CDS
CCTACTATCGTTGCAGTATG+GGG 0.587972 2.3:-45154081 MS.gene062065:CDS
TTATTGTTATTATCATATGG+TGG 0.589100 2.3:+45155872 None:intergenic
TGAGGGTAATGACCTTGGAA+TGG 0.589839 2.3:+45152259 None:intergenic
AACTGCCGAGTAGGTAACAC+TGG 0.590392 2.3:+45154485 None:intergenic
TCATCAAAGAACTGCCGAGT+AGG 0.608428 2.3:+45154476 None:intergenic
GAAGGGAAATACACAAGATG+CGG 0.615352 2.3:-45154513 MS.gene062065:CDS
AATAACGCTTCCTTGAGAGA+AGG 0.619409 2.3:+45152151 None:intergenic
TATGGACAAAAGACAGCCAA+GGG 0.623915 2.3:-45154121 MS.gene062065:CDS
ATATGGACAAAAGACAGCCA+AGG 0.629423 2.3:-45154122 MS.gene062065:CDS
GCATGTGAGTACATTAATGA+AGG 0.632412 2.3:-45154374 MS.gene062065:CDS
CTGCAACGATAGTAGGCTCG+TGG 0.637106 2.3:+45154088 None:intergenic
CAATTACCTAGTAAGAGTAG+TGG 0.638666 2.3:-45152073 MS.gene062065:CDS
GATAGTAGGCTCGTGGACAA+GGG 0.644110 2.3:+45154095 None:intergenic
GGAAAACAATGAAAACAACA+TGG 0.645971 2.3:-45154408 MS.gene062065:CDS
TGTTCATCACAACCTATGAA+GGG 0.646804 2.3:-45152564 MS.gene062065:CDS
GGATTTCCTTGAATTTGATG+GGG 0.651214 2.3:+45155375 None:intergenic
AATAGAGGACCAATCATCTG+AGG 0.655874 2.3:-45154336 MS.gene062065:CDS
AGAATCTCCATTTGTGAACG+AGG 0.664014 2.3:+45152117 None:intergenic
GAAAACAATGAAAACAACAT+GGG 0.672904 2.3:-45154407 MS.gene062065:CDS
TTGTTGTAGATTTGTTGATG+AGG 0.675654 2.3:+45152462 None:intergenic
AATTGCTGGAAGTTGATGAA+AGG 0.676322 2.3:-45155895 MS.gene062065:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATCAAATATCAAATA+TGG + chr2.3:45152215-45152234 None:intergenic 10.0%
!!! AATAACATTTTTTAGAATTT+AGG + chr2.3:45154106-45154125 None:intergenic 10.0%
!!! ATCTATTTTCAAATATTATA+AGG - chr2.3:45154818-45154837 MS.gene062065:intron 10.0%
!!! TCTATTTTCAAATATTATAA+GGG - chr2.3:45154819-45154838 MS.gene062065:intron 10.0%
!!! TTTTATATGTATAATAGAAA+TGG - chr2.3:45154288-45154307 MS.gene062065:CDS 10.0%
!! CAAAATATAAGAATTTCAAT+TGG - chr2.3:45154058-45154077 MS.gene062065:CDS 15.0%
!! TTAAATTTCTATCAAAGATA+TGG - chr2.3:45154523-45154542 MS.gene062065:intron 15.0%
!! TTCTTCATAATTAATTAGAA+GGG - chr2.3:45153438-45153457 MS.gene062065:intron 15.0%
!! TTTCTTCATAATTAATTAGA+AGG - chr2.3:45153437-45153456 MS.gene062065:intron 15.0%
!!! AAAAGTTTTTGTAATGTAAT+TGG + chr2.3:45154470-45154489 None:intergenic 15.0%
!!! AATTTTAATGCATTGAAAAA+GGG - chr2.3:45154411-45154430 MS.gene062065:CDS 15.0%
!!! ATTAATTTGTAATGTTTAGA+TGG - chr2.3:45153801-45153820 MS.gene062065:intron 15.0%
!!! ATTTTCAAATATTTCGAAAA+TGG - chr2.3:45154790-45154809 MS.gene062065:intron 15.0%
!!! ATTTTTGTTTTTTATGTTTG+TGG - chr2.3:45154140-45154159 MS.gene062065:CDS 15.0%
!!! GATAAATTAAACTCTAATTT+TGG - chr2.3:45153323-45153342 MS.gene062065:intron 15.0%
!!! TCTTTATTGTTATTATCATA+TGG + chr2.3:45152102-45152121 None:intergenic 15.0%
!!! TTTTCAAATATTTCGAAAAT+GGG - chr2.3:45154791-45154810 MS.gene062065:intron 15.0%
!! AAAATTCAAGAACTAACTAT+TGG + chr2.3:45155079-45155098 None:intergenic 20.0%
!! AATAAGATTAATCACTTACT+TGG + chr2.3:45152625-45152644 None:intergenic 20.0%
!! ATTTAATGATCTCATGTATT+TGG - chr2.3:45153094-45153113 MS.gene062065:intron 20.0%
!! TAAAATCAAGTGTAATTGTA+GGG + chr2.3:45155627-45155646 None:intergenic 20.0%
!! TAACCGAACAATAAATAAAA+TGG - chr2.3:45154674-45154693 MS.gene062065:intron 20.0%
!! TAGGTTATATCATATCAAAA+TGG + chr2.3:45153967-45153986 None:intergenic 20.0%
!! TATGAAGAAAAAATGTACAA+AGG + chr2.3:45153427-45153446 None:intergenic 20.0%
!! TGAAATAAATAGCAAAATAG+AGG - chr2.3:45153617-45153636 MS.gene062065:intron 20.0%
!! TTAATTACATTGCAATTAAC+AGG - chr2.3:45155354-45155373 MS.gene062065:intron 20.0%
!! TTGAATTGTAAATAGTTACT+TGG + chr2.3:45155658-45155677 None:intergenic 20.0%
!! TTTATCTATTTGTAAGTAGA+CGG + chr2.3:45153173-45153192 None:intergenic 20.0%
!! TTTCAAATATTTCGAAAATG+GGG - chr2.3:45154792-45154811 MS.gene062065:intron 20.0%
!!! AATTCTCATTTCATTCTTTT+AGG + chr2.3:45155020-45155039 None:intergenic 20.0%
!!! ACATTTTTTAGAATTTAGGT+TGG + chr2.3:45154102-45154121 None:intergenic 20.0%
!!! ATAATGATGAGACTATTTTA+AGG - chr2.3:45155246-45155265 MS.gene062065:intron 20.0%
!!! ATTTTAATGCATTGAAAAAG+GGG - chr2.3:45154412-45154431 MS.gene062065:CDS 20.0%
!!! CAATTTTAATGCATTGAAAA+AGG - chr2.3:45154410-45154429 MS.gene062065:CDS 20.0%
!!! CATTTTTTAGAATTTAGGTT+GGG + chr2.3:45154101-45154120 None:intergenic 20.0%
!!! CTTCCATTTTATTTATTGTT+CGG + chr2.3:45154680-45154699 None:intergenic 20.0%
!!! TAATAACTTGACATTTTGAT+GGG - chr2.3:45152507-45152526 MS.gene062065:CDS 20.0%
!!! TCAATTGATAATATATGCAA+AGG - chr2.3:45154586-45154605 MS.gene062065:intron 20.0%
!!! TGTGTAATTCTGTTTTAAAA+TGG - chr2.3:45155282-45155301 MS.gene062065:intron 20.0%
!!! TGTTGTTTATGTAATTAACA+TGG - chr2.3:45153249-45153268 MS.gene062065:intron 20.0%
!!! TTAAACTCTAATTTTGGATT+TGG - chr2.3:45153329-45153348 MS.gene062065:intron 20.0%
!!! TTACAAGTTGAAAATATCAT+AGG + chr2.3:45153382-45153401 None:intergenic 20.0%
!!! TTATTGTTATTATCATATGG+TGG + chr2.3:45152099-45152118 None:intergenic 20.0%
! AAAGTCATGAATACATCATA+AGG - chr2.3:45155222-45155241 MS.gene062065:intron 25.0%
! AAATGAGTGTGAATCAAATA+AGG + chr2.3:45153047-45153066 None:intergenic 25.0%
! AAATGTCAAGTTATTAGGTT+AGG + chr2.3:45152503-45152522 None:intergenic 25.0%
! AAGGAGAATGAAAATTTAAG+AGG - chr2.3:45152877-45152896 MS.gene062065:intron 25.0%
! AATTGTAAATAGTTACTTGG+AGG + chr2.3:45155655-45155674 None:intergenic 25.0%
! ATGTAATGAATGTTTGTGAA+AGG - chr2.3:45154704-45154723 MS.gene062065:intron 25.0%
! ATTAATCACTTACTTGGATT+TGG + chr2.3:45152619-45152638 None:intergenic 25.0%
! ATTATAAGGAAGATGATGAT+GGG - chr2.3:45154950-45154969 MS.gene062065:intron 25.0%
! ATTGTAAATAGTTACTTGGA+GGG + chr2.3:45155654-45155673 None:intergenic 25.0%
! CATCAAAATGTCAAGTTATT+AGG + chr2.3:45152508-45152527 None:intergenic 25.0%
! CTCTCATTGTTTATCATATT+TGG + chr2.3:45155751-45155770 None:intergenic 25.0%
! GAAAACAATGAAAACAACAT+GGG - chr2.3:45153561-45153580 MS.gene062065:intron 25.0%
! GAAAACTTACAGTATTCTTA+TGG + chr2.3:45152158-45152177 None:intergenic 25.0%
! GTAAAATCAAGTGTAATTGT+AGG + chr2.3:45155628-45155647 None:intergenic 25.0%
! TAGCTTATGATGAAAATCAT+GGG - chr2.3:45152414-45152433 MS.gene062065:CDS 25.0%
! TCAACAAATCTACAACAATA+TGG - chr2.3:45155511-45155530 MS.gene062065:intron 25.0%
! TCAGATTGTAGCAAAAATAA+TGG - chr2.3:45153525-45153544 MS.gene062065:intron 25.0%
! TTAATCACTTACTTGGATTT+GGG + chr2.3:45152618-45152637 None:intergenic 25.0%
! TTAGCTTATGATGAAAATCA+TGG - chr2.3:45152413-45152432 MS.gene062065:CDS 25.0%
! TTATCTATTTGTAAGTAGAC+GGG + chr2.3:45153172-45153191 None:intergenic 25.0%
! TTCCTTATAATGCTAATTAG+TGG + chr2.3:45154941-45154960 None:intergenic 25.0%
!! AAGAGTCATATACTTTGTTT+AGG - chr2.3:45152305-45152324 MS.gene062065:CDS 25.0%
!! ATGACTCTTTATAAAGCATT+AGG + chr2.3:45152294-45152313 None:intergenic 25.0%
!! CTAATAACTTGACATTTTGA+TGG - chr2.3:45152506-45152525 MS.gene062065:CDS 25.0%
!! TCGAACTTATGATAGTTTTT+AGG + chr2.3:45153986-45154005 None:intergenic 25.0%
!!! TTATTTTTGCTACAATCTGA+AGG + chr2.3:45153525-45153544 None:intergenic 25.0%
!!! TTTGATAATTGCTCTAAGAT+TGG - chr2.3:45152823-45152842 MS.gene062065:intron 25.0%
ACTTACAGTATTCTTATGGA+AGG + chr2.3:45152154-45152173 None:intergenic 30.0%
ATAATATATGCAAAGGTCTG+GGG - chr2.3:45154593-45154612 MS.gene062065:intron 30.0%
ATGAATGTTTGTGAAAGGAA+AGG - chr2.3:45154709-45154728 MS.gene062065:intron 30.0%
ATTCAAGAACTAACTATTGG+AGG + chr2.3:45155076-45155095 None:intergenic 30.0%
CATTATAAGGAAGATGATGA+TGG - chr2.3:45154949-45154968 MS.gene062065:intron 30.0%
CTTAATAAAAGAGTAGATCC+TGG + chr2.3:45152545-45152564 None:intergenic 30.0%
GATAATATATGCAAAGGTCT+GGG - chr2.3:45154592-45154611 MS.gene062065:intron 30.0%
GGAAAACAATGAAAACAACA+TGG - chr2.3:45153560-45153579 MS.gene062065:intron 30.0%
GTAATGTTTAGATGGTTGAT+GGG - chr2.3:45153809-45153828 MS.gene062065:intron 30.0%
TAATCACTTACTTGGATTTG+GGG + chr2.3:45152617-45152636 None:intergenic 30.0%
TAATCCAGTATACACAAACA+TGG + chr2.3:45152188-45152207 None:intergenic 30.0%
TGATAATATATGCAAAGGTC+TGG - chr2.3:45154591-45154610 MS.gene062065:intron 30.0%
TGCCACTAATTAGCATTATA+AGG - chr2.3:45154936-45154955 MS.gene062065:intron 30.0%
TGTAATGTTTAGATGGTTGA+TGG - chr2.3:45153808-45153827 MS.gene062065:intron 30.0%
TTGTAGTGCATCATCAATTA+AGG + chr2.3:45154377-45154396 None:intergenic 30.0%
! CGGACAAACTATTAATTTGA+GGG - chr2.3:45154200-45154219 MS.gene062065:intron 30.0%
! GAAGTTGTGTTGTGTTATTA+AGG + chr2.3:45152574-45152593 None:intergenic 30.0%
! GACTATTAGTTCTTACTAGA+TGG - chr2.3:45154548-45154567 MS.gene062065:intron 30.0%
! GATCAGATTTTGCATTTGTA+AGG - chr2.3:45153691-45153710 MS.gene062065:intron 30.0%
! GGACAAACTATTAATTTGAG+GGG - chr2.3:45154201-45154220 MS.gene062065:intron 30.0%
! GGGTATTGTTTCTCATATTA+GGG - chr2.3:45154839-45154858 MS.gene062065:intron 30.0%
! GTAAATGAGACAAGTTTTCA+TGG + chr2.3:45155178-45155197 None:intergenic 30.0%
! GTAGCTATAACATTTGAGTT+TGG + chr2.3:45155583-45155602 None:intergenic 30.0%
! GTTTTCATTGTTTTCCACTA+TGG + chr2.3:45153556-45153575 None:intergenic 30.0%
! TATTAGTTCTTACTAGATGG+AGG - chr2.3:45154551-45154570 MS.gene062065:intron 30.0%
! TCGATTTTATAGAAGTGAGT+TGG - chr2.3:45154027-45154046 MS.gene062065:intron 30.0%
! TCTTCAGAATCGTTTACTTT+TGG - chr2.3:45152933-45152952 MS.gene062065:intron 30.0%
! TTTAAGAGGTATGCTAAATC+TGG - chr2.3:45152891-45152910 MS.gene062065:intron 30.0%
!! AAATAATGGATTTGCCATAG+TGG - chr2.3:45153539-45153558 MS.gene062065:intron 30.0%
!! AGCAAGTTGTGATTATTTTG+AGG + chr2.3:45152988-45153007 None:intergenic 30.0%
!! AGGGTATTGTTTCTCATATT+AGG - chr2.3:45154838-45154857 MS.gene062065:intron 30.0%
!! GCAAATATGTTACTTTGTTC+TGG - chr2.3:45154344-45154363 MS.gene062065:CDS 30.0%
!! TGTTCTAAAGACACTCTTTA+AGG - chr2.3:45154876-45154895 MS.gene062065:intron 30.0%
!! TTGTTGTAGATTTGTTGATG+AGG + chr2.3:45155509-45155528 None:intergenic 30.0%
!!! AATTTTGGATTTGGTGAATG+TGG - chr2.3:45153338-45153357 MS.gene062065:intron 30.0%
!!! CATCTTTTTTGGTTGTGTTT+GGG + chr2.3:45154625-45154644 None:intergenic 30.0%
!!! TTCTTTTAGGTTGAATGTGT+AGG + chr2.3:45155007-45155026 None:intergenic 30.0%
AACACAACCAAAAAAGATGG+AGG - chr2.3:45154626-45154645 MS.gene062065:intron 35.0%
AATGACCTTGGAATGGAAAA+GGG + chr2.3:45155705-45155724 None:intergenic 35.0%
AATGTTTGTGAAAGGAAAGG+TGG - chr2.3:45154712-45154731 MS.gene062065:intron 35.0%
AGTATTCTTATGGAAGGAAG+AGG + chr2.3:45152148-45152167 None:intergenic 35.0%
ATGTTTGTGAAAGGAAAGGT+GGG - chr2.3:45154713-45154732 MS.gene062065:intron 35.0%
ATTGCAATTAACAGGTGCAA+AGG - chr2.3:45155362-45155381 MS.gene062065:intron 35.0%
CAATTACCTAGTAAGAGTAG+TGG - chr2.3:45155895-45155914 MS.gene062065:CDS 35.0%
CACTACTCTTACTAGGTAAT+TGG + chr2.3:45155897-45155916 None:intergenic 35.0%
CCAAACACAACCAAAAAAGA+TGG - chr2.3:45154623-45154642 MS.gene062065:intron 35.0%
GAAACACCTCAAATGATTGA+GGG + chr2.3:45155729-45155748 None:intergenic 35.0%
GCATGTGAGTACATTAATGA+AGG - chr2.3:45153594-45153613 MS.gene062065:intron 35.0%
GGATTTCCTTGAATTTGATG+GGG + chr2.3:45152596-45152615 None:intergenic 35.0%
GGGATTTCCTTGAATTTGAT+GGG + chr2.3:45152597-45152616 None:intergenic 35.0%
TAATGACCTTGGAATGGAAA+AGG + chr2.3:45155706-45155725 None:intergenic 35.0%
TAGTGTTGTCGATCATCAAT+TGG - chr2.3:45153283-45153302 MS.gene062065:intron 35.0%
TATGATGAAAATCATGGGTG+TGG - chr2.3:45152419-45152438 MS.gene062065:CDS 35.0%
TATGCCATGTTTGTGTATAC+TGG - chr2.3:45152181-45152200 MS.gene062065:CDS 35.0%
TCATTACCCTCAATCATTTG+AGG - chr2.3:45155720-45155739 MS.gene062065:intron 35.0%
TCGACAACACTACAACATAT+AGG + chr2.3:45153275-45153294 None:intergenic 35.0%
TGTTCATCACAACCTATGAA+GGG - chr2.3:45155404-45155423 MS.gene062065:CDS 35.0%
TTAATGCATTGAAAAAGGGG+AGG - chr2.3:45154415-45154434 MS.gene062065:CDS 35.0%
TTGAGCATCTAATGCTTTAC+AGG - chr2.3:45152337-45152356 MS.gene062065:CDS 35.0%
! CAATGAGAGAAAACTTGCTT+TGG - chr2.3:45155762-45155781 MS.gene062065:intron 35.0%
! GCGGACAAACTATTAATTTG+AGG - chr2.3:45154199-45154218 MS.gene062065:intron 35.0%
! GCTATAACATTTGAGTTTGG+TGG + chr2.3:45155580-45155599 None:intergenic 35.0%
! TCTTACTAGGTAATTGGCTT+TGG + chr2.3:45155891-45155910 None:intergenic 35.0%
! TTCCAAAGCACTTCAAACTT+AGG - chr2.3:45154442-45154461 MS.gene062065:CDS 35.0%
! TTTGAGTTTGGTGGAGATAA+TGG + chr2.3:45155571-45155590 None:intergenic 35.0%
!! AAATAATCACAACTTGCTGC+TGG - chr2.3:45152990-45153009 MS.gene062065:intron 35.0%
!! AATTGCTGGAAGTTGATGAA+AGG - chr2.3:45152073-45152092 MS.gene062065:CDS 35.0%
!! GATGATGTTAGGTTTGCTAT+TGG + chr2.3:45155463-45155482 None:intergenic 35.0%
!! TGTAGATTTGTTGATGAGGT+AGG + chr2.3:45155505-45155524 None:intergenic 35.0%
!!! ACTTTCACCTCCATCTTTTT+TGG + chr2.3:45154636-45154655 None:intergenic 35.0%
!!! CCATCTTTTTTGGTTGTGTT+TGG + chr2.3:45154626-45154645 None:intergenic 35.0%
!!! GTTGTGATTATTTTGAGGCA+AGG + chr2.3:45152983-45153002 None:intergenic 35.0%
AAAATCTGATCGTGCCCTTA+TGG + chr2.3:45153682-45153701 None:intergenic 40.0%
AATAACGCTTCCTTGAGAGA+AGG + chr2.3:45155820-45155839 None:intergenic 40.0%
AATAGAGGACCAATCATCTG+AGG - chr2.3:45153632-45153651 MS.gene062065:intron 40.0%
AGAAACCAGTGTTACCTACT+CGG - chr2.3:45153478-45153497 MS.gene062065:intron 40.0%
AGAATCTCCATTTGTGAACG+AGG + chr2.3:45155854-45155873 None:intergenic 40.0%
AGAGAAAGATCCTTCTCTCA+AGG - chr2.3:45155807-45155826 MS.gene062065:intron 40.0%
AGTGTAATTGTAGGGCATGT+TGG + chr2.3:45155619-45155638 None:intergenic 40.0%
AGTTTACCTGCTTACGAACT+GGG + chr2.3:45153921-45153940 None:intergenic 40.0%
ATATGGACAAAAGACAGCCA+AGG - chr2.3:45153846-45153865 MS.gene062065:intron 40.0%
ATGATTATGGCTCCCTTCAT+AGG + chr2.3:45155419-45155438 None:intergenic 40.0%
ATGATTGAGGGTAATGACCT+TGG + chr2.3:45155717-45155736 None:intergenic 40.0%
CAACTTCCCCATCAAATTCA+AGG - chr2.3:45152587-45152606 MS.gene062065:CDS 40.0%
CCTCTCTATTGAGAATGATG+CGG - chr2.3:45154180-45154199 MS.gene062065:intron 40.0%
GAAGCTTGATGAAGCAAAGA+AGG - chr2.3:45152858-45152877 MS.gene062065:intron 40.0%
GAAGGGAAATACACAAGATG+CGG - chr2.3:45153455-45153474 MS.gene062065:intron 40.0%
GGAAACACCTCAAATGATTG+AGG + chr2.3:45155730-45155749 None:intergenic 40.0%
GGGGATTTCCTTGAATTTGA+TGG + chr2.3:45152598-45152617 None:intergenic 40.0%
GGGTGTCAATGGAGAAAATA+TGG - chr2.3:45153829-45153848 MS.gene062065:intron 40.0%
GTGTTCATCACAACCTATGA+AGG - chr2.3:45155403-45155422 MS.gene062065:CDS 40.0%
TAGTTTACCTGCTTACGAAC+TGG + chr2.3:45153922-45153941 None:intergenic 40.0%
TATGGACAAAAGACAGCCAA+GGG - chr2.3:45153847-45153866 MS.gene062065:intron 40.0%
TGTATGAATCGAGATCATCG+AGG + chr2.3:45152239-45152258 None:intergenic 40.0%
TTGCTACAATCTGAAGGTGA+AGG + chr2.3:45153519-45153538 None:intergenic 40.0%
! AGCCTAAGTTTGAAGTGCTT+TGG + chr2.3:45154447-45154466 None:intergenic 40.0%
! GAGATAATGGTGAAGAGAAG+AGG + chr2.3:45155558-45155577 None:intergenic 40.0%
!! CTGGTGGAAGTTGATGATTA+TGG + chr2.3:45155432-45155451 None:intergenic 40.0%
!! GATTTGTTGATGAGGTAGGA+AGG + chr2.3:45155501-45155520 None:intergenic 40.0%
!! TAAGGAAGATGATGATGGGA+AGG - chr2.3:45154954-45154973 MS.gene062065:intron 40.0%
!! TGTTGAGTGCTGATGATGTT+AGG + chr2.3:45155474-45155493 None:intergenic 40.0%
!!! TTTGCTATTGGTTTTGCTGC+TGG + chr2.3:45155451-45155470 None:intergenic 40.0%
AGCCTACTATCGTTGCAGTA+TGG - chr2.3:45153885-45153904 MS.gene062065:intron 45.0%
ATTGTAGGGCATGTTGGAGA+TGG + chr2.3:45155613-45155632 None:intergenic 45.0%
CCGCATCATTCTCAATAGAG+AGG + chr2.3:45154183-45154202 None:intergenic 45.0%
CCTACTATCGTTGCAGTATG+GGG - chr2.3:45153887-45153906 MS.gene062065:intron 45.0%
GCCTACTATCGTTGCAGTAT+GGG - chr2.3:45153886-45153905 MS.gene062065:intron 45.0%
TCATCAAAGAACTGCCGAGT+AGG + chr2.3:45153495-45153514 None:intergenic 45.0%
TCCATTTGTGAACGAGGACA+TGG + chr2.3:45155848-45155867 None:intergenic 45.0%
TGAGGGTAATGACCTTGGAA+TGG + chr2.3:45155712-45155731 None:intergenic 45.0%
! AGGACCAATCATCTGAGGTT+GGG - chr2.3:45153637-45153656 MS.gene062065:intron 45.0%
! GAGAGCTAGAGTGTCCATAA+GGG - chr2.3:45153665-45153684 MS.gene062065:intron 45.0%
! TTGCTCCCTTTTCCATTCCA+AGG - chr2.3:45155697-45155716 MS.gene062065:intron 45.0%
!! ACAGGTGCAAAGGTGTTTCA+AGG - chr2.3:45155372-45155391 MS.gene062065:CDS 45.0%
!! AGATGGTTGATGGGTGTCAA+TGG - chr2.3:45153818-45153837 MS.gene062065:intron 45.0%
AACTGCCGAGTAGGTAACAC+TGG + chr2.3:45153486-45153505 None:intergenic 50.0%
CCCCATACTGCAACGATAGT+AGG + chr2.3:45153890-45153909 None:intergenic 50.0%
GATAGTAGGCTCGTGGACAA+GGG + chr2.3:45153876-45153895 None:intergenic 50.0%
GATGGGAAAATCATGCAGCC+AGG - chr2.3:45152524-45152543 MS.gene062065:CDS 50.0%
GCCATGTCCTCGTTCACAAA+TGG - chr2.3:45155844-45155863 MS.gene062065:CDS 50.0%
TGCACCCAACCTCAGATGAT+TGG + chr2.3:45153644-45153663 None:intergenic 50.0%
TTCATGCCCAGTTCGTAAGC+AGG - chr2.3:45153912-45153931 MS.gene062065:intron 50.0%
! AATCATCTGAGGTTGGGTGC+AGG - chr2.3:45153643-45153662 MS.gene062065:intron 50.0%
! GAGGACCAATCATCTGAGGT+TGG - chr2.3:45153636-45153655 MS.gene062065:intron 50.0%
! GGAGAGCTAGAGTGTCCATA+AGG - chr2.3:45153664-45153683 MS.gene062065:intron 50.0%
!!! GCTATTGGTTTTGCTGCTGG+TGG + chr2.3:45155448-45155467 None:intergenic 50.0%
CGATAGTAGGCTCGTGGACA+AGG + chr2.3:45153877-45153896 None:intergenic 55.0%
CTGCAACGATAGTAGGCTCG+TGG + chr2.3:45153883-45153902 None:intergenic 55.0%
GGAAAGGTGGGAAGCGAACT+AGG - chr2.3:45154725-45154744 MS.gene062065:intron 55.0%
TCGTGGACAAGGGTTGCCCT+TGG + chr2.3:45153866-45153885 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 45152069 45155921 45152069 ID=MS.gene062065
chr2.3 mRNA 45152069 45155921 45152069 ID=MS.gene062065.t1;Parent=MS.gene062065
chr2.3 exon 45155823 45155921 45155823 ID=MS.gene062065.t1.exon1;Parent=MS.gene062065.t1
chr2.3 CDS 45155823 45155921 45155823 ID=cds.MS.gene062065.t1;Parent=MS.gene062065.t1
chr2.3 exon 45155365 45155448 45155365 ID=MS.gene062065.t1.exon2;Parent=MS.gene062065.t1
chr2.3 CDS 45155365 45155448 45155365 ID=cds.MS.gene062065.t1;Parent=MS.gene062065.t1
chr2.3 exon 45154979 45155149 45154979 ID=MS.gene062065.t1.exon3;Parent=MS.gene062065.t1
chr2.3 CDS 45154979 45155149 45154979 ID=cds.MS.gene062065.t1;Parent=MS.gene062065.t1
chr2.3 exon 45154283 45154534 45154283 ID=MS.gene062065.t1.exon4;Parent=MS.gene062065.t1
chr2.3 CDS 45154283 45154534 45154283 ID=cds.MS.gene062065.t1;Parent=MS.gene062065.t1
chr2.3 exon 45154057 45154170 45154057 ID=MS.gene062065.t1.exon5;Parent=MS.gene062065.t1
chr2.3 CDS 45154057 45154170 45154057 ID=cds.MS.gene062065.t1;Parent=MS.gene062065.t1
chr2.3 exon 45152069 45152614 45152069 ID=MS.gene062065.t1.exon6;Parent=MS.gene062065.t1
chr2.3 CDS 45152069 45152614 45152069 ID=cds.MS.gene062065.t1;Parent=MS.gene062065.t1
Gene Sequence

>MS.gene062065

ATGAAATTGCTGGAAGTTGATGAAAGGCCACCATATGATAATAACAATAAAGATCTACAAAACAAGATCGATGAATCCTCTTCCTTCCATAAGAATACTGTAAGTTTTCATTTATGCCATGTTTGTGTATACTGGATTAATTTCCATATTTGATATTTGATTATTAGCCTCGATGATCTCGATTCATACATAAAATTTACAATTTTGTTCATTGCTTTATTTCCTAATGCTTTATAAAGAGTCATATACTTTGTTTAGGTAAATAGACTTGAGCATCTAATGCTTTACAGGTTGAAGTAGTTTTTTTTCTTTTTAAAGAAGTTGAAGTAGTTTTTGTTCTTTTGTTAGCTTATGATGAAAATCATGGGTGTGGACAAAGATTTCATCTCATCATTCTTTTATCTCAATTACTTATTTTGTTAACAAAAAAACCTAACCTAATAACTTGACATTTTGATGGGAAAATCATGCAGCCAGGATCTACTCTTTTATTAAGTTTGAGCCTTAATAACACAACACAACTTCCCCATCAAATTCAAGGAAATCCCCAAATCCAAGTAAGTGATTAATCTTATTAGTGAATTTAAAGCATGTTTTTTTTAATTAACTTTCACACATAAATTTTAATTTATTTATATGAGAGTATATAAAGTAATTAATCTTATTAGTGAATTAAGAGCATGTGTTATTTTTAATTAACTTTCACACATAAATTTAAATTTATTTATATGATAGTATAAAAGTAATTTTTTGATTTGATAATTGCTCTAAGATTGGCAATATACGAGTGAAGCTTGATGAAGCAAAGAAGGAGAATGAAAATTTAAGAGGTATGCTAAATCTGGTCAACGATCGATGCAATGTTCTTCAGAATCGTTTACTTTTGGCTATGCATATGCATCAATCATCTTCCTTGCCTCAAAATAATCACAACTTGCTGCTGGTAAGTTTGACTTCAAAATTTTGTTCACATATCCTTATTTGATTCACACTCATTTTTTTGTTCTATTAAATTTGCTTGAAATATTTAATGATCTCATGTATTTGGTCAACACGTCTTAATATTTTCAATACTTATAGCTACAATTAATTAATTATATCCCGTCTACTTACAAATAGATAAACACAAAAAATAAACAAATAATTAATGATAATAAAAAATTTGAAACGTATAATTTTTTGTTGTTTATGTAATTAACATGGCCTATATGTTGTAGTGTTGTCGATCATCAATTGGTTAGACACATTCAATATGATAAATTAAACTCTAATTTTGGATTTGGTGAATGTGGTCAATTAAACTTTTTCAACCTATGATATTTTCAACTTGTAATAAATATAATTGCATATATTAACCTTTGTACATTTTTTCTTCATAATTAATTAGAAGGGAAATACACAAGATGCGGAGAAACCAGTGTTACCTACTCGGCAGTTCTTTGATGAGCCTTCACCTTCAGATTGTAGCAAAAATAATGGATTTGCCATAGTGGAAAACAATGAAAACAACATGGGAAGAAATCTTGCATGTGAGTACATTAATGAAGGTGAAATAAATAGCAAAATAGAGGACCAATCATCTGAGGTTGGGTGCAGGAGAGCTAGAGTGTCCATAAGGGCACGATCAGATTTTGCATTTGTAAGGACATGCATGCAACTCTTCGTTTTTTAATTAGTACAATAATCTAATAATATAGTAATCAATAATGATTAATTTGTATTTTAAGTGTTAATTAATTTGTAATGTTTAGATGGTTGATGGGTGTCAATGGAGAAAATATGGACAAAAGACAGCCAAGGGCAACCCTTGTCCACGAGCCTACTATCGTTGCAGTATGGGGACTTCATGCCCAGTTCGTAAGCAGGTAAACTATTATTTAATTTCATAGATCGTACCATTTTGATATGATATAACCTAAAAACTATCATAAGTTCGAGTTTTTTTTAAATCTTATAAATCGATTTTATAGAAGTGAGTTGGCTTTTATGCAAAATATAAGAATTTCAATTGGAAATCGTTAAATGTTAGCCCAACCTAAATTCTAAAAAATGTTATTTCAAAAATTGAACAATTTTTGTTTTTTATGTTTGTGGTATCACTTCACTTGTTTCCTCTCTATTGAGAATGATGCGGACAAACTATTAATTTGAGGGGAAAATTAAAGAAAAAAATACAATGTTGTCAATATATTTTTGTTTTGCAATTTTTTTTGTCTTGTTTTTATATGTATAATAGAAATGGAGAAAGTACAATATTTTATATCTTGATATTTGAGCAAATATGTTACTTTGTTCTGGTGTTCGTCCTTAATTGATGATGCACTACAATGAGACATCACAACAATTTTAATGCATTGAAAAAGGGGAGGAACATTCCAAAGCACTTCAAACTTAGGCTCCAATTACATTACAAAAACTTTTTCAGTTTTTATCTTTTAAAATTTTATGTTCATTTTAAATTTCTATCAAAGATATGGAAGACTATTAGTTCTTACTAGATGGAGGTGAAAGAATAGTTCAATTGATAATATATGCAAAGGTCTGGGGTTCGAACCCAAACACAACCAAAAAAGATGGAGGTGAAAGTTTCGTATTAATAACATGATAACCGAACAATAAATAAAATGGAAGACTCATGTAATGAATGTTTGTGAAAGGAAAGGTGGGAAGCGAACTAGGAAGAACACATTTTCAAAGTTAATGAAAAAAGATGTATTTAGCATTTTCAAATATTTCGAAAATGGGGTTCATCTATTTTCAAATATTATAAGGGTATTGTTTCTCATATTAGGGTCTTTTTAAACGAGTGTTCTAAAGACACTCTTTAAGGATTCTCATTTAATAAACTATCTATTTAAAGTTCTTTATGCCACTAATTAGCATTATAAGGAAGATGATGATGGGAAGGAAACAATTATTTATTTGAGCTTTGATACCTACACATTCAACCTAAAAGAATGAAATGAGAATTTCTATAGCTCATATTCTTGCAGTAATTGATTATCCTCCAATAGTTAGTTCTTGAATTTTGCAACACTCTGAATAAACATTATTTTAACAAATTTTTCACTTATTTGTAGTCTCAAATATGTACTTCTGTTCAAAACCATGAAAACTTGTCTCATTTACTAATTGTAAGTGCACTTTTCATAAAAAGTCATGAATACATCATAAGGAATAATGATGAGACTATTTTAAGGATCAAATTATTGTTGTGTAATTCTGTTTTAAAATGGTGTCTATATGAATGAATTTTTTTGCATATTGTTAACTCAATATATGATATTAATTACATTGCAATTAACAGGTGCAAAGGTGTTTCAAGGATGAGAGTGTGTTCATCACAACCTATGAAGGGAGCCATAATCATCAACTTCCACCAGCAGCAAAACCAATAGCAAACCTAACATCATCAGCACTCAACACATTCCTTCCTACCTCATCAACAAATCTACAACAATATGGCAACAATCTAACAAATACATTCCTCTTCTCTTCACCATTATCTCCACCAAACTCAAATGTTATAGCTACTTTCTCTCCATCTCCAACATGCCCTACAATTACACTTGATTTTACACTCCCTCCAAGTAACTATTTACAATTCAAAAATCATAAACAATCATCATTGCTCCCTTTTCCATTCCAAGGTCATTACCCTCAATCATTTGAGGTGTTTCCAAATATGATAAACAATGAGAGAAAACTTGCTTTGGTTGATGTTGTGAGTGAAGCTCTAGAGAAAGATCCTTCTCTCAAGGAAGCGTTATTTTCAGCCATGTCCTCGTTCACAAATGGAGATTCTTCAAATATTAATAACCAAAGCCAATTACCTAGTAAGAGTAGTGGTTAA
Protein sequence

>MS.gene062065.t1

MKLLEVDERPPYDNNNKDLQNKIDESSSFHKNTPGSTLLLSLSLNNTTQLPHQIQGNPQIQIGNIRVKLDEAKKENENLRGMLNLVNDRCNVLQNRLLLAMHMHQSSSLPQNNHNLLLKGNTQDAEKPVLPTRQFFDEPSPSDCSKNNGFAIVENNENNMGRNLACEYINEGEINSKIEDQSSEVGCRRARVSIRARSDFAFMVDGCQWRKYGQKTAKGNPCPRAYYRCSMGTSCPVRKQVQRCFKDESVFITTYEGSHNHQLPPAAKPIANLTSSALNTFLPTSSTNLQQYGNNLTNTFLFSSPLSPPNSNVIATFSPSPTCPTITLDFTLPPSNYLQFKNHKQSSLLPFPFQGHYPQSFEVFPNMINNERKLALVDVVSEALEKDPSLKEALFSAMSSFTNGDSSNINNQSQLPSKSSG