AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063159

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063159.t1 MTR_3g116770 90.000 400 23 4 1 397 1 386 0.0 737
MS.gene063159.t1 MTR_3g116770 89.750 400 23 5 1 397 1 385 0.0 730
MS.gene063159.t1 MTR_4g070320 52.228 404 106 19 1 392 1 329 1.49e-113 335
MS.gene063159.t1 MTR_5g048860 44.727 275 119 9 132 394 290 543 1.01e-53 187
MS.gene063159.t1 MTR_7g053410 43.945 289 138 8 114 394 235 507 7.82e-52 181
MS.gene063159.t1 MTR_7g053410 43.945 289 138 8 114 394 235 507 1.34e-51 181
MS.gene063159.t1 MTR_1g017350 64.062 128 46 0 162 289 220 347 1.88e-49 172
MS.gene063159.t1 MTR_1g017350 64.062 128 46 0 162 289 220 347 2.16e-49 172
MS.gene063159.t1 MTR_8g065740 56.287 167 60 3 132 289 230 392 2.17e-49 173
MS.gene063159.t1 MTR_1g017350 46.445 211 78 4 172 382 230 405 2.82e-49 172
MS.gene063159.t1 MTR_1g017350 44.796 221 87 4 162 382 220 405 3.24e-49 172
MS.gene063159.t1 MTR_8g012290 43.144 299 130 11 114 396 211 485 7.24e-49 172
MS.gene063159.t1 MTR_8g012290 50.691 217 77 7 114 321 211 406 3.87e-48 171
MS.gene063159.t1 MTR_8g062240 58.904 146 48 3 164 303 108 247 4.18e-42 149
MS.gene063159.t1 MTR_5g037250 51.852 162 64 4 135 290 41 194 7.39e-42 147
MS.gene063159.t1 MTR_3g498825 80.899 89 17 0 186 274 131 219 3.48e-41 148
MS.gene063159.t1 MTR_7g092510 61.017 118 38 1 178 295 157 266 4.46e-41 148
MS.gene063159.t1 MTR_8g099880 82.558 86 14 1 197 281 140 225 9.89e-41 147
MS.gene063159.t1 MTR_1g059270 65.347 101 35 0 195 295 153 253 6.37e-40 145
MS.gene063159.t1 MTR_1g059270 67.010 97 32 0 195 291 153 249 6.67e-40 142
MS.gene063159.t1 MTR_6g084120 60.227 88 24 2 198 283 151 229 5.07e-25 103
MS.gene063159.t1 MTR_4g131160 72.727 66 17 1 202 267 196 260 1.95e-22 98.6
MS.gene063159.t1 MTR_4g131160 72.727 66 17 1 202 267 199 263 2.15e-22 98.2
MS.gene063159.t1 MTR_1g080890 69.697 66 19 1 202 267 193 257 3.03e-21 94.4
MS.gene063159.t1 MTR_4g065870 41.071 112 60 2 161 266 308 419 8.33e-16 79.0
MS.gene063159.t1 MTR_2g098310 55.224 67 30 0 200 266 330 396 1.39e-15 78.2
MS.gene063159.t1 MTR_4g033295 55.385 65 29 0 202 266 118 182 3.23e-15 73.9
MS.gene063159.t1 MTR_4g065870 40.367 109 59 2 161 263 308 416 3.90e-15 77.0
MS.gene063159.t1 MTR_2g098310 56.061 66 29 0 200 265 330 395 4.13e-15 77.0
MS.gene063159.t1 MTR_1g069155 52.174 69 32 1 204 272 319 386 5.29e-15 77.0
MS.gene063159.t1 MTR_4g033295 53.731 67 30 1 202 267 118 184 6.53e-15 72.8
MS.gene063159.t1 MTR_7g092540 34.615 156 89 6 131 275 163 316 7.61e-15 75.1
MS.gene063159.t1 MTR_1g052470 53.623 69 32 0 202 270 298 366 1.40e-14 75.1
MS.gene063159.t1 MTR_1g084980 45.205 73 39 1 204 276 464 535 1.46e-14 75.9
MS.gene063159.t1 MTR_1g070870 65.152 66 22 1 202 267 120 184 2.19e-14 73.6
MS.gene063159.t1 MTR_1g070870 65.152 66 22 1 202 267 123 187 2.26e-14 73.6
MS.gene063159.t1 MTR_7g092540 34.932 146 82 6 131 265 163 306 3.05e-14 73.2
MS.gene063159.t1 MTR_7g099540 50.000 68 33 1 205 272 255 321 2.14e-13 72.0
MS.gene063159.t1 MTR_3g028520 46.753 77 41 0 190 266 310 386 4.89e-13 70.5
MS.gene063159.t1 MTR_3g027650 46.753 77 41 0 190 266 310 386 4.89e-13 70.5
MS.gene063159.t1 MTR_8g036235 49.231 65 33 0 202 266 316 380 1.91e-12 68.6
MS.gene063159.t1 MTR_3g027650 47.297 74 39 0 190 263 310 383 2.40e-12 68.6
MS.gene063159.t1 MTR_1g019240 47.692 65 33 1 204 268 87 150 2.40e-12 67.0
MS.gene063159.t1 MTR_4g107320 47.368 76 38 2 220 293 60 135 2.47e-12 64.3
MS.gene063159.t1 MTR_1g018090 44.828 87 45 2 180 266 254 337 4.42e-12 67.4
MS.gene063159.t1 MTR_3g449770 42.857 91 49 3 179 267 284 373 1.40e-11 66.2
MS.gene063159.t1 MTR_7g099540 49.206 63 31 1 205 267 255 316 1.56e-11 66.2
MS.gene063159.t1 MTR_3g103030 36.567 134 72 3 133 266 172 292 3.82e-11 64.3
MS.gene063159.t1 MTR_7g110810 26.423 246 143 9 33 278 70 277 4.00e-11 64.3
MS.gene063159.t1 MTR_7g111320 39.806 103 57 3 169 267 395 496 4.68e-11 65.1
MS.gene063159.t1 MTR_5g017040 53.571 56 25 1 213 268 116 170 6.71e-11 63.5
MS.gene063159.t1 MTR_5g005110 50.000 64 31 1 203 266 225 287 6.90e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063159.t1 AT4G34530 38.441 372 157 12 22 377 15 330 1.24e-56 189
MS.gene063159.t1 AT1G68920 45.560 259 108 8 142 390 234 469 4.40e-53 184
MS.gene063159.t1 AT1G68920 45.560 259 108 8 142 390 234 469 4.40e-53 184
MS.gene063159.t1 AT1G68920 45.560 259 108 8 142 390 234 469 4.40e-53 184
MS.gene063159.t1 AT1G68920 50.239 209 80 6 183 390 283 468 6.62e-53 183
MS.gene063159.t1 AT3G07340 53.927 191 77 4 198 385 254 436 1.28e-51 179
MS.gene063159.t1 AT2G18300 43.836 292 106 13 90 378 91 327 4.14e-51 174
MS.gene063159.t1 AT3G07340 53.158 190 80 3 198 384 254 437 5.39e-51 177
MS.gene063159.t1 AT4G36540 41.993 281 100 11 22 291 2 230 2.38e-50 172
MS.gene063159.t1 AT2G18300 52.500 200 73 7 90 287 91 270 4.23e-50 172
MS.gene063159.t1 AT1G10120 46.396 222 73 6 160 381 171 346 9.02e-50 172
MS.gene063159.t1 AT1G10120 46.396 222 73 6 160 381 171 346 9.02e-50 172
MS.gene063159.t1 AT2G18300 52.500 200 73 7 90 287 91 270 1.15e-49 172
MS.gene063159.t1 AT4G36540 42.238 277 97 11 22 287 2 226 1.71e-49 169
MS.gene063159.t1 AT3G23690 61.111 144 49 4 148 289 140 278 6.38e-48 167
MS.gene063159.t1 AT1G26260 58.503 147 48 1 143 289 126 259 1.21e-46 163
MS.gene063159.t1 AT5G48560 68.595 121 36 2 198 317 297 416 1.92e-46 166
MS.gene063159.t1 AT1G26260 58.503 147 48 1 143 289 155 288 2.21e-46 163
MS.gene063159.t1 AT5G48560 68.595 121 36 2 198 317 297 416 3.26e-46 166
MS.gene063159.t1 AT1G26260 58.503 147 48 1 143 289 177 310 4.71e-46 163
MS.gene063159.t1 AT1G26260 58.503 147 48 1 143 289 177 310 4.71e-46 163
MS.gene063159.t1 AT1G26260 58.503 147 48 1 143 289 177 310 4.71e-46 163
MS.gene063159.t1 AT1G26260 58.503 147 48 1 143 289 177 310 4.71e-46 163
MS.gene063159.t1 AT5G50915 63.359 131 43 3 154 281 88 216 2.89e-45 157
MS.gene063159.t1 AT5G50915 63.359 131 43 3 154 281 88 216 2.89e-45 157
MS.gene063159.t1 AT5G50915 64.516 124 41 2 159 281 95 216 1.23e-44 156
MS.gene063159.t1 AT5G50915 64.516 124 41 2 159 281 95 216 1.23e-44 156
MS.gene063159.t1 AT1G59640 52.198 182 61 6 139 318 79 236 7.89e-43 150
MS.gene063159.t1 AT1G59640 54.938 162 63 3 139 298 79 232 3.55e-42 151
MS.gene063159.t1 AT3G57800 50.296 169 76 2 168 329 170 337 5.20e-42 152
MS.gene063159.t1 AT2G42300 70.968 93 27 0 196 288 179 271 1.19e-41 148
MS.gene063159.t1 AT2G42300 63.810 105 38 0 196 300 179 283 3.58e-40 145
MS.gene063159.t1 AT5G62610 81.579 76 14 0 198 273 149 224 3.26e-37 136
MS.gene063159.t1 AT5G62610 81.579 76 14 0 198 273 149 224 3.26e-37 136
MS.gene063159.t1 AT1G73830 48.630 146 62 1 134 279 92 224 5.03e-35 130
MS.gene063159.t1 AT1G73830 48.630 146 62 1 134 279 92 224 5.60e-35 130
MS.gene063159.t1 AT1G18400 75.000 80 20 0 200 279 143 222 6.59e-35 129
MS.gene063159.t1 AT1G18400 75.000 80 20 0 200 279 143 222 1.28e-34 129
MS.gene063159.t1 AT1G25330 65.217 92 32 0 198 289 100 191 1.82e-33 125
MS.gene063159.t1 AT3G57800 39.352 216 76 3 168 329 170 384 4.15e-33 129
MS.gene063159.t1 AT5G58010 65.000 80 23 2 202 277 99 177 2.09e-23 99.8
MS.gene063159.t1 AT2G24260 71.212 66 18 1 202 267 35 99 5.92e-23 97.1
MS.gene063159.t1 AT4G30980 72.727 66 17 1 202 267 130 194 1.41e-22 97.4
MS.gene063159.t1 AT4G30980 72.727 66 17 1 202 267 178 242 2.26e-22 97.8
MS.gene063159.t1 AT2G24260 71.212 66 18 1 202 267 138 202 4.24e-22 96.7
MS.gene063159.t1 AT2G42300 76.087 46 11 0 196 241 179 224 5.38e-18 82.8
MS.gene063159.t1 AT2G42300 76.087 46 11 0 196 241 179 224 5.38e-18 82.8
MS.gene063159.t1 AT2G42300 76.087 46 11 0 196 241 179 224 6.37e-18 82.8
MS.gene063159.t1 AT2G20180 39.423 104 57 2 164 267 174 271 1.02e-15 78.6
MS.gene063159.t1 AT2G20180 39.423 104 57 2 164 267 174 271 1.02e-15 78.6
MS.gene063159.t1 AT2G20180 39.423 104 57 2 164 267 174 271 1.02e-15 78.6
MS.gene063159.t1 AT2G20180 39.423 104 57 2 164 267 245 342 1.45e-15 78.6
MS.gene063159.t1 AT2G20180 39.423 104 57 2 164 267 245 342 1.45e-15 78.6
MS.gene063159.t1 AT2G20180 39.423 104 57 2 164 267 245 342 1.45e-15 78.6
MS.gene063159.t1 AT2G20180 39.423 104 57 2 164 267 245 342 1.45e-15 78.6
MS.gene063159.t1 AT2G42280 35.878 131 60 3 144 265 227 342 1.74e-15 77.8
MS.gene063159.t1 AT2G42280 36.842 133 58 4 144 265 227 344 1.86e-15 77.4
MS.gene063159.t1 AT4G28790 27.451 255 117 10 87 337 195 385 1.35e-14 75.5
MS.gene063159.t1 AT1G51140 50.633 79 35 2 191 265 289 367 2.45e-14 74.3
MS.gene063159.t1 AT1G03040 60.274 73 28 1 195 267 132 203 4.36e-14 72.8
MS.gene063159.t1 AT4G09180 50.746 67 33 0 200 266 182 248 4.47e-14 72.4
MS.gene063159.t1 AT1G03040 60.274 73 28 1 195 267 137 208 5.69e-14 72.4
MS.gene063159.t1 AT1G09530 50.000 64 31 1 204 267 339 401 5.86e-14 73.9
MS.gene063159.t1 AT1G09530 50.000 64 31 1 204 267 339 401 5.86e-14 73.9
MS.gene063159.t1 AT1G09530 50.000 64 31 1 204 267 339 401 5.86e-14 73.9
MS.gene063159.t1 AT1G09530 50.000 64 31 1 204 267 339 401 5.86e-14 73.9
MS.gene063159.t1 AT1G09530 50.000 64 31 1 204 267 339 401 5.86e-14 73.9
MS.gene063159.t1 AT1G09530 50.000 64 31 1 204 267 339 401 5.86e-14 73.9
MS.gene063159.t1 AT4G28800 33.803 142 69 3 205 346 252 368 6.68e-14 73.6
MS.gene063159.t1 AT1G35460 52.308 65 31 0 202 266 181 245 6.89e-14 71.6
MS.gene063159.t1 AT2G20180 38.614 101 56 2 164 264 245 339 6.96e-14 72.8
MS.gene063159.t1 AT4G02590 63.636 66 23 1 202 267 83 147 7.78e-14 71.2
MS.gene063159.t1 AT1G03040 60.274 73 28 1 195 267 137 208 1.01e-13 72.0
MS.gene063159.t1 AT4G28815 56.897 58 24 1 205 262 71 127 1.02e-13 70.5
MS.gene063159.t1 AT4G02590 63.636 66 23 1 202 267 146 210 1.05e-13 71.6
MS.gene063159.t1 AT4G02590 63.636 66 23 1 202 267 146 210 1.05e-13 71.6
MS.gene063159.t1 AT4G28815 56.897 58 24 1 205 262 147 203 3.34e-13 70.1
MS.gene063159.t1 AT4G28790 28.962 183 90 5 87 269 195 337 4.08e-12 67.4
MS.gene063159.t1 AT2G46970 40.000 100 53 3 180 274 166 263 4.48e-12 67.4
MS.gene063159.t1 AT4G28800 52.459 61 28 1 205 265 264 323 4.74e-12 67.8
MS.gene063159.t1 AT4G33880 41.935 93 51 2 176 266 238 329 4.80e-12 67.4
MS.gene063159.t1 AT2G46970 40.000 100 53 3 180 274 197 294 4.80e-12 67.4
MS.gene063159.t1 AT2G46970 40.000 100 53 3 180 274 175 272 4.86e-12 67.4
MS.gene063159.t1 AT4G00050 50.769 65 31 1 202 266 105 168 1.83e-11 65.1
MS.gene063159.t1 AT2G43140 50.000 64 32 0 202 265 134 197 2.40e-11 63.2
MS.gene063159.t1 AT2G43140 50.000 64 32 0 202 265 231 294 3.27e-11 64.3
MS.gene063159.t1 AT4G00050 50.769 65 31 1 202 266 207 270 3.53e-11 64.7
MS.gene063159.t1 AT4G00050 50.769 65 31 1 202 266 207 270 3.83e-11 64.7
MS.gene063159.t1 AT4G36930 50.820 61 29 1 208 268 197 256 4.31e-11 64.3
MS.gene063159.t1 AT1G27740 51.562 64 30 1 203 266 168 230 4.61e-11 63.2

Find 68 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACAGCAATTTGTTCCTTA+TGG 0.249421 3.4:+99385792 MS.gene063159:CDS
AATTGAAGTTGTATAGGTTT+TGG 0.253509 3.4:-99386055 None:intergenic
TCTCAATCTCAGTTATTTAC+AGG 0.262036 3.4:+99386107 MS.gene063159:CDS
CTCATTCACTCTTCAATTGC+TGG 0.307759 3.4:+99384706 MS.gene063159:CDS
AATTGAAGAGTGAATGAGTT+TGG 0.318103 3.4:-99384701 None:intergenic
AAAGTTTGAGGAATGGATAT+AGG 0.336253 3.4:-99385873 None:intergenic
GATCAAAATTGAAGTTGTAT+AGG 0.339897 3.4:-99386061 None:intergenic
ATTGGGATTCCCGGTGATCT+TGG 0.342453 3.4:-99384973 None:intergenic
AAGTTTGAGGAATGGATATA+GGG 0.345771 3.4:-99385872 None:intergenic
ATTGAAGAGTGAATGAGTTT+GGG 0.357408 3.4:-99384700 None:intergenic
TGGAATGAACCCTACAGATA+TGG 0.359058 3.4:+99385827 MS.gene063159:CDS
TTGAATACTACGACTAGTTC+CGG 0.373329 3.4:+99384403 MS.gene063159:CDS
CCAGGATGCAACAAAATTAC+AGG 0.386637 3.4:+99385302 MS.gene063159:CDS
AAAGACAAGAGGATCAAAAT+AGG 0.388939 3.4:+99384933 MS.gene063159:CDS
TATGTATGATGCAGGTTAGA+AGG 0.402202 3.4:+99385237 MS.gene063159:intron
TTGCTTGTTGTTTAGGCTGT+TGG 0.409201 3.4:+99384897 MS.gene063159:intron
CTTCTAAGTCCCATATCTGT+AGG 0.410368 3.4:-99385837 None:intergenic
TCACCTTCCCATGTTGTAGA+AGG 0.416145 3.4:-99386029 None:intergenic
AGCTATGGCTGTCGGTGGCT+TGG 0.431984 3.4:-99385127 None:intergenic
ACTCTCAGTTGCTAAATTCC+CGG 0.447140 3.4:-99384422 None:intergenic
CACGCACACACCTTGAGCTT+AGG 0.457558 3.4:-99384784 None:intergenic
TGCTCGAACATGAATGTAAT+CGG 0.461397 3.4:-99385096 None:intergenic
GAAGAATCAATAAAAGTTTG+AGG 0.465715 3.4:-99385885 None:intergenic
AGAGTTGGCATTTGAAGAGT+TGG 0.474811 3.4:-99384619 None:intergenic
TGAATACTACGACTAGTTCC+GGG 0.477955 3.4:+99384404 MS.gene063159:CDS
ATCAATAAAAGTTTGAGGAA+TGG 0.483827 3.4:-99385880 None:intergenic
AACAAAATTACAGGAAAAGC+TGG 0.485342 3.4:+99385311 MS.gene063159:CDS
GAGTGAATGAGTTTGGGGTC+TGG 0.491043 3.4:-99384694 None:intergenic
GGAATGAACCCTACAGATAT+GGG 0.494720 3.4:+99385828 MS.gene063159:CDS
ATGTATGATGCAGGTTAGAA+GGG 0.501649 3.4:+99385238 MS.gene063159:intron
CTACAGATATGGGACTTAGA+AGG 0.505239 3.4:+99385838 MS.gene063159:CDS
TTTGCATTTGCATCACAAGT+AGG 0.509857 3.4:-99385705 None:intergenic
TTCTAAGTCCCATATCTGTA+GGG 0.511108 3.4:-99385836 None:intergenic
TCATTCACTCTTCAATTGCT+GGG 0.512372 3.4:+99384707 MS.gene063159:CDS
TCAATATTGAAATCAAGCCT+TGG 0.519925 3.4:-99385531 None:intergenic
CCAGCATCTAATCCACCATA+AGG 0.520595 3.4:-99385807 None:intergenic
GAAGTAGTAACATCAAGAGT+TGG 0.521259 3.4:-99384634 None:intergenic
AACAGTGTTTGAAAGACAAA+GGG 0.521570 3.4:+99384459 MS.gene063159:CDS
CAGCAATTTGTTCCTTATGG+TGG 0.522613 3.4:+99385795 MS.gene063159:CDS
TTACTCTTTCAGCTAAGCTA+TGG 0.530789 3.4:-99385142 None:intergenic
AGAAGATACTTCAAACTCAA+AGG 0.553682 3.4:+99385043 MS.gene063159:CDS
TTCAGCTAAGCTATGGCTGT+CGG 0.556309 3.4:-99385135 None:intergenic
TAACAGTGTTTGAAAGACAA+AGG 0.558772 3.4:+99384458 MS.gene063159:CDS
CCTTATGGTGGATTAGATGC+TGG 0.561668 3.4:+99385807 MS.gene063159:CDS
TATCTGCAAGATTTAGTACC+AGG 0.562856 3.4:+99385284 MS.gene063159:CDS
AATTATACCTTCTACAACAT+GGG 0.569534 3.4:+99386022 MS.gene063159:CDS
GACGGCGAATCCAAGATCAC+CGG 0.574848 3.4:+99384963 MS.gene063159:CDS
AGTTTGGGGTCTGGAAGAAG+AGG 0.575334 3.4:-99384685 None:intergenic
TCATCATAATCCTAAGCTCA+AGG 0.584084 3.4:+99384774 MS.gene063159:CDS
GTAAATAACTGAGATTGAGA+AGG 0.584711 3.4:-99386104 None:intergenic
TAAATCAGCAGCAAGTGAAG+AGG 0.586756 3.4:-99384593 None:intergenic
TTCAAACACTGTTATGTCAG+AGG 0.587410 3.4:-99384449 None:intergenic
GGTGAATTGAACTGAAGATA+AGG 0.590017 3.4:-99385771 None:intergenic
AAATTATACCTTCTACAACA+TGG 0.591829 3.4:+99386021 MS.gene063159:CDS
TTGGGGTCTGGAAGAAGAGG+TGG 0.593623 3.4:-99384682 None:intergenic
AGCTAAGCTATGGCTGTCGG+TGG 0.605688 3.4:-99385132 None:intergenic
AATTAGCTACTGTTAATCCA+AGG 0.613412 3.4:+99385514 MS.gene063159:CDS
ATACCTTCTACAACATGGGA+AGG 0.615789 3.4:+99386026 MS.gene063159:CDS
ACGGCGAATCCAAGATCACC+GGG 0.628691 3.4:+99384964 MS.gene063159:CDS
ATCAAAATAGGTGCTGAGGA+CGG 0.629527 3.4:+99384945 MS.gene063159:CDS
TTGAAGAGTGAATGAGTTTG+GGG 0.630480 3.4:-99384699 None:intergenic
GACAAAGGGAAAGAATGAAG+TGG 0.633675 3.4:+99384473 MS.gene063159:CDS
GAGGATCAAAATAGGTGCTG+AGG 0.635802 3.4:+99384941 MS.gene063159:CDS
AGTTTGAGGAATGGATATAG+GGG 0.638076 3.4:-99385871 None:intergenic
AAGTTGAGAACAAAGACAAG+AGG 0.638388 3.4:+99384922 MS.gene063159:CDS
ATTCATGTTCGAGCACGTCG+TGG 0.642577 3.4:+99385104 MS.gene063159:CDS
TTGAGCTTAGGATTATGATG+AGG 0.647091 3.4:-99384772 None:intergenic
CCATAAGGAACAAATTGCTG+TGG 0.664587 3.4:-99385792 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAAGTGAATTAAT+TGG + chr3.4:99384841-99384860 MS.gene063159:intron 10.0%
!! TAAGTAGTAATAATATACTT+TGG + chr3.4:99385162-99385181 MS.gene063159:intron 15.0%
!!! TAACATAAACAAATTTGTTA+TGG - chr3.4:99385468-99385487 None:intergenic 15.0%
!! AAAATTAAAACACTAGTAAC+GGG - chr3.4:99385950-99385969 None:intergenic 20.0%
!! ATTAAGCTAAAATACAGATT+AGG - chr3.4:99385615-99385634 None:intergenic 20.0%
!! CAAAATTAAAACACTAGTAA+CGG - chr3.4:99385951-99385970 None:intergenic 20.0%
!! TAATTATATATGTATGATGC+AGG + chr3.4:99385229-99385248 MS.gene063159:intron 20.0%
!!! ATAAACAAATTTGTTATGGA+TGG - chr3.4:99385464-99385483 None:intergenic 20.0%
!!! GTGTTTTAATTTTGTTGTAT+TGG + chr3.4:99385957-99385976 MS.gene063159:intron 20.0%
! AAATTATACCTTCTACAACA+TGG + chr3.4:99386021-99386040 MS.gene063159:CDS 25.0%
! AATTATACCTTCTACAACAT+GGG + chr3.4:99386022-99386041 MS.gene063159:CDS 25.0%
! ACAAAAATATTAACAGAGAG+TGG - chr3.4:99385927-99385946 None:intergenic 25.0%
! ATCAATAAAAGTTTGAGGAA+TGG - chr3.4:99385883-99385902 None:intergenic 25.0%
! GAAGAATCAATAAAAGTTTG+AGG - chr3.4:99385888-99385907 None:intergenic 25.0%
! GATCAAAATTGAAGTTGTAT+AGG - chr3.4:99386064-99386083 None:intergenic 25.0%
! TGATGATCTATTTGAAAAAG+AGG + chr3.4:99385551-99385570 MS.gene063159:CDS 25.0%
!! AATTGAAGTTGTATAGGTTT+TGG - chr3.4:99386058-99386077 None:intergenic 25.0%
!!! AAATGCAAATTTTCAAGCTA+TGG + chr3.4:99385719-99385738 MS.gene063159:CDS 25.0%
!!! AATGCAAATTTTCAAGCTAT+GGG + chr3.4:99385720-99385739 MS.gene063159:CDS 25.0%
!!! TAATTTGTTGCTTGTTGTTT+AGG + chr3.4:99384890-99384909 MS.gene063159:intron 25.0%
!!! TTGTTGTTCTTTTTGGTATT+GGG - chr3.4:99384993-99385012 None:intergenic 25.0%
AAAGACAAGAGGATCAAAAT+AGG + chr3.4:99384933-99384952 MS.gene063159:CDS 30.0%
AAAGTTTGAGGAATGGATAT+AGG - chr3.4:99385876-99385895 None:intergenic 30.0%
AACAAAATTACAGGAAAAGC+TGG + chr3.4:99385311-99385330 MS.gene063159:CDS 30.0%
AACAGTGTTTGAAAGACAAA+GGG + chr3.4:99384459-99384478 MS.gene063159:CDS 30.0%
AAGTTTGAGGAATGGATATA+GGG - chr3.4:99385875-99385894 None:intergenic 30.0%
AATTAGCTACTGTTAATCCA+AGG + chr3.4:99385514-99385533 MS.gene063159:CDS 30.0%
AATTGAAGAGTGAATGAGTT+TGG - chr3.4:99384704-99384723 None:intergenic 30.0%
AGAAGATACTTCAAACTCAA+AGG + chr3.4:99385043-99385062 MS.gene063159:CDS 30.0%
ATTGAAGAGTGAATGAGTTT+GGG - chr3.4:99384703-99384722 None:intergenic 30.0%
CATAGATAGATCAATGTGAT+AGG - chr3.4:99385439-99385458 None:intergenic 30.0%
GGTAATAAAGAATATGCAGA+TGG + chr3.4:99385572-99385591 MS.gene063159:intron 30.0%
GTAAATAACTGAGATTGAGA+AGG - chr3.4:99386107-99386126 None:intergenic 30.0%
TAACAGTGTTTGAAAGACAA+AGG + chr3.4:99384458-99384477 MS.gene063159:CDS 30.0%
TCAATATTGAAATCAAGCCT+TGG - chr3.4:99385534-99385553 None:intergenic 30.0%
!! ACTTCAATTTTGATCAAGCA+AGG + chr3.4:99386069-99386088 MS.gene063159:CDS 30.0%
!! CTTCAATTTTGATCAAGCAA+GGG + chr3.4:99386070-99386089 MS.gene063159:CDS 30.0%
!! GTTGTTGTTCTTTTTGGTAT+TGG - chr3.4:99384994-99385013 None:intergenic 30.0%
!! GTTGTTGTTGTTGTTCTTTT+TGG - chr3.4:99385000-99385019 None:intergenic 30.0%
!! TTTGTGTTGATTCCTTACAT+AGG + chr3.4:99385677-99385696 MS.gene063159:intron 30.0%
AAGTAGGAAACACCTATGTA+AGG - chr3.4:99385692-99385711 None:intergenic 35.0%
AAGTTGAGAACAAAGACAAG+AGG + chr3.4:99384922-99384941 MS.gene063159:CDS 35.0%
AGTTTGAGGAATGGATATAG+GGG - chr3.4:99385874-99385893 None:intergenic 35.0%
ATGTATGATGCAGGTTAGAA+GGG + chr3.4:99385238-99385257 MS.gene063159:intron 35.0%
CAACAACAACAACAACAACA+AGG + chr3.4:99384496-99384515 MS.gene063159:CDS 35.0%
GAAGTAGTAACATCAAGAGT+TGG - chr3.4:99384637-99384656 None:intergenic 35.0%
GGTGAATTGAACTGAAGATA+AGG - chr3.4:99385774-99385793 None:intergenic 35.0%
GTCTCTTCAAAAACAAGTTG+AGG + chr3.4:99385364-99385383 MS.gene063159:CDS 35.0%
TATCTGCAAGATTTAGTACC+AGG + chr3.4:99385284-99385303 MS.gene063159:CDS 35.0%
TATGTATGATGCAGGTTAGA+AGG + chr3.4:99385237-99385256 MS.gene063159:intron 35.0%
TCATCATAATCCTAAGCTCA+AGG + chr3.4:99384774-99384793 MS.gene063159:CDS 35.0%
TCATTCACTCTTCAATTGCT+GGG + chr3.4:99384707-99384726 MS.gene063159:CDS 35.0%
TGCTCGAACATGAATGTAAT+CGG - chr3.4:99385099-99385118 None:intergenic 35.0%
TTACTCTTTCAGCTAAGCTA+TGG - chr3.4:99385145-99385164 None:intergenic 35.0%
TTCAAACACTGTTATGTCAG+AGG - chr3.4:99384452-99384471 None:intergenic 35.0%
TTCTAAGTCCCATATCTGTA+GGG - chr3.4:99385839-99385858 None:intergenic 35.0%
TTGAAGAGTGAATGAGTTTG+GGG - chr3.4:99384702-99384721 None:intergenic 35.0%
TTGAGCTTAGGATTATGATG+AGG - chr3.4:99384775-99384794 None:intergenic 35.0%
TTTGCATTTGCATCACAAGT+AGG - chr3.4:99385708-99385727 None:intergenic 35.0%
! GATTCTTCTTGTTTCTCTGT+AGG + chr3.4:99385900-99385919 MS.gene063159:intron 35.0%
! GGATTATGATGAGGTTTTTC+AGG - chr3.4:99384766-99384785 None:intergenic 35.0%
! TTGAATACTACGACTAGTTC+CGG + chr3.4:99384403-99384422 MS.gene063159:CDS 35.0%
ACTCTCAGTTGCTAAATTCC+CGG - chr3.4:99384425-99384444 None:intergenic 40.0%
AGAGTTGGCATTTGAAGAGT+TGG - chr3.4:99384622-99384641 None:intergenic 40.0%
ATACCTTCTACAACATGGGA+AGG + chr3.4:99386026-99386045 MS.gene063159:CDS 40.0%
CCAGGATGCAACAAAATTAC+AGG + chr3.4:99385302-99385321 MS.gene063159:CDS 40.0%
CCATAAGGAACAAATTGCTG+TGG - chr3.4:99385795-99385814 None:intergenic 40.0%
CTACAGATATGGGACTTAGA+AGG + chr3.4:99385838-99385857 MS.gene063159:CDS 40.0%
CTCATTCACTCTTCAATTGC+TGG + chr3.4:99384706-99384725 MS.gene063159:CDS 40.0%
CTTCTAAGTCCCATATCTGT+AGG - chr3.4:99385840-99385859 None:intergenic 40.0%
GACAAAGGGAAAGAATGAAG+TGG + chr3.4:99384473-99384492 MS.gene063159:CDS 40.0%
GGAATGAACCCTACAGATAT+GGG + chr3.4:99385828-99385847 MS.gene063159:CDS 40.0%
TAAATCAGCAGCAAGTGAAG+AGG - chr3.4:99384596-99384615 None:intergenic 40.0%
TGGAATGAACCCTACAGATA+TGG + chr3.4:99385827-99385846 MS.gene063159:CDS 40.0%
! CAGCAATTTGTTCCTTATGG+TGG + chr3.4:99385795-99385814 MS.gene063159:CDS 40.0%
! CCACAGCAATTTGTTCCTTA+TGG + chr3.4:99385792-99385811 MS.gene063159:CDS 40.0%
! CCTGTAATTTTGTTGCATCC+TGG - chr3.4:99385305-99385324 None:intergenic 40.0%
! TGAATACTACGACTAGTTCC+GGG + chr3.4:99384404-99384423 MS.gene063159:CDS 40.0%
!! ATCAAAATAGGTGCTGAGGA+CGG + chr3.4:99384945-99384964 MS.gene063159:CDS 40.0%
!! CTTTTTGGTATTGGGATTCC+CGG - chr3.4:99384985-99385004 None:intergenic 40.0%
!! TTGCTTGTTGTTTAGGCTGT+TGG + chr3.4:99384897-99384916 MS.gene063159:intron 40.0%
CCAGCATCTAATCCACCATA+AGG - chr3.4:99385810-99385829 None:intergenic 45.0%
TCACCTTCCCATGTTGTAGA+AGG - chr3.4:99386032-99386051 None:intergenic 45.0%
TTCAGCTAAGCTATGGCTGT+CGG - chr3.4:99385138-99385157 None:intergenic 45.0%
! GAGGATCAAAATAGGTGCTG+AGG + chr3.4:99384941-99384960 MS.gene063159:CDS 45.0%
!! CCTTATGGTGGATTAGATGC+TGG + chr3.4:99385807-99385826 MS.gene063159:CDS 45.0%
ATTCATGTTCGAGCACGTCG+TGG + chr3.4:99385104-99385123 MS.gene063159:CDS 50.0%
ATTGGGATTCCCGGTGATCT+TGG - chr3.4:99384976-99384995 None:intergenic 50.0%
! AGTTTGGGGTCTGGAAGAAG+AGG - chr3.4:99384688-99384707 None:intergenic 50.0%
! GAGTGAATGAGTTTGGGGTC+TGG - chr3.4:99384697-99384716 None:intergenic 50.0%
ACGGCGAATCCAAGATCACC+GGG + chr3.4:99384964-99384983 MS.gene063159:CDS 55.0%
AGCTAAGCTATGGCTGTCGG+TGG - chr3.4:99385135-99385154 None:intergenic 55.0%
CACGCACACACCTTGAGCTT+AGG - chr3.4:99384787-99384806 None:intergenic 55.0%
GACGGCGAATCCAAGATCAC+CGG + chr3.4:99384963-99384982 MS.gene063159:CDS 55.0%
TTGGGGTCTGGAAGAAGAGG+TGG - chr3.4:99384685-99384704 None:intergenic 55.0%
AGCTATGGCTGTCGGTGGCT+TGG - chr3.4:99385130-99385149 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 99384391 99386128 99384391 ID=MS.gene063159
chr3.4 mRNA 99384391 99386128 99384391 ID=MS.gene063159.t1;Parent=MS.gene063159
chr3.4 exon 99384391 99384795 99384391 ID=MS.gene063159.t1.exon1;Parent=MS.gene063159.t1
chr3.4 CDS 99384391 99384795 99384391 ID=cds.MS.gene063159.t1;Parent=MS.gene063159.t1
chr3.4 exon 99384912 99385160 99384912 ID=MS.gene063159.t1.exon2;Parent=MS.gene063159.t1
chr3.4 CDS 99384912 99385160 99384912 ID=cds.MS.gene063159.t1;Parent=MS.gene063159.t1
chr3.4 exon 99385251 99385385 99385251 ID=MS.gene063159.t1.exon3;Parent=MS.gene063159.t1
chr3.4 CDS 99385251 99385385 99385251 ID=cds.MS.gene063159.t1;Parent=MS.gene063159.t1
chr3.4 exon 99385501 99385572 99385501 ID=MS.gene063159.t1.exon4;Parent=MS.gene063159.t1
chr3.4 CDS 99385501 99385572 99385501 ID=cds.MS.gene063159.t1;Parent=MS.gene063159.t1
chr3.4 exon 99385699 99385917 99385699 ID=MS.gene063159.t1.exon5;Parent=MS.gene063159.t1
chr3.4 CDS 99385699 99385917 99385699 ID=cds.MS.gene063159.t1;Parent=MS.gene063159.t1
chr3.4 exon 99386017 99386128 99386017 ID=MS.gene063159.t1.exon6;Parent=MS.gene063159.t1
chr3.4 CDS 99386017 99386128 99386017 ID=cds.MS.gene063159.t1;Parent=MS.gene063159.t1
Gene Sequence

>MS.gene063159

ATGTTGCACTGTTTGAATACTACGACTAGTTCCGGGAATTTAGCAACTGAGAGTTGCTCCTCTGACATAACAGTGTTTGAAAGACAAAGGGAAAGAATGAAGTGGCAACAACAACAACAACAACAAGGTTACTTCAACGCTGCTGCTATGTTCTGTTCTTCTCTGCAACAACAACAACAACAACAAGTTGACAATTCTGAAGCCTCTTCACTTGCTGCTGATTTACTGCCAACTCTTCAAATGCCAACTCTTGATGTTACTACTTCTGCTATTTCAAGAACTTTCAGCTGTCCACCTCTTCTTCCAGACCCCAAACTCATTCACTCTTCAATTGCTGGGAAAGACAAACACAATTCTTCTAAGAAAAGAAAACCTGAAAAACCTCATCATAATCCTAAGCTCAAGGTGTGTGCGTGCAATTTTGCATAGCAATATCATTGATCTTCAAGAAATAAATAAAGTGAATTAATTGGCTTAAATAACTATAAGAAATTATATTTAATTTGTTGCTTGTTGTTTAGGCTGTTGGTGAAGTTGAGAACAAAGACAAGAGGATCAAAATAGGTGCTGAGGACGGCGAATCCAAGATCACCGGGAATCCCAATACCAAAAAGAACAACAACAACAACAACAACAAAGAAAACTGTGTAGCAGAAGATACTTCAAACTCAAAGGAAAATTCAAAAGTTTCTGATGTTCAAAAGACCGATTACATTCATGTTCGAGCACGTCGTGGCCAAGCCACCGACAGCCATAGCTTAGCTGAAAGAGTAAGTAGTAATAATATACTTTGGTTTCTGTCTGACTGAGAAAAGTAAAATCAAAGTATCTGAATAATTAATTATATATGTATGATGCAGGTTAGAAGGGAAAAGATTAGTGAGAGAATGAAGTATCTGCAAGATTTAGTACCAGGATGCAACAAAATTACAGGAAAAGCTGGAATGCTTGATGAAATCATCAACTATGTTCAGTCTCTTCAAAAACAAGTTGAGGTAATTAAAAGAGTTAATTAATTAAGTTCATTACATTGATTCTTAATTTGCCTATCACATTGATCTATCTATGATCCATCCATAACAAATTTGTTTATGTTAATTTTCTTTGCAGTTCTTGTCAATGAAATTAGCTACTGTTAATCCAAGGCTTGATTTCAATATTGATGATCTATTTGAAAAAGAGGTAATAAAGAATATGCAGATGGAAGAATTTCACTTTTCACCTAATCTGTATTTTAGCTTAATTTGAAATCTGTAATATAATATTAATATTAATATATTCTTCTATTTGTGTTGATTCCTTACATAGGTGTTTCCTACTTGTGATGCAAATGCAAATTTTCAAGCTATGGGAATGTCAACAGAATTGAATAGTAACAATCCTTATCTTCAGTTCAATTCACCACAGCAATTTGTTCCTTATGGTGGATTAGATGCTGGAATGAACCCTACAGATATGGGACTTAGAAGGAGCATAAGTGCCCCTATATCCATTCCTCAAACTTTTATTGATTCTTCTTGTTTCTCTGTAGGTCCACTCTCTGTTAATATTTTTGTCCCGTTACTAGTGTTTTAATTTTGTTGTATTGGTTTATTTTATTAATTTTATCTTTGTTTATGTGTGTAGCAGCAAATTATACCTTCTACAACATGGGAAGGTGATTTCCAAAACCTATACAACTTCAATTTTGATCAAGCAAGGGCAACATCTTTTCCTTCTCAATCTCAGTTATTTACAG
Protein sequence

>MS.gene063159.t1

MLHCLNTTTSSGNLATESCSSDITVFERQRERMKWQQQQQQQGYFNAAAMFCSSLQQQQQQQVDNSEASSLAADLLPTLQMPTLDVTTSAISRTFSCPPLLPDPKLIHSSIAGKDKHNSSKKRKPEKPHHNPKLKAVGEVENKDKRIKIGAEDGESKITGNPNTKKNNNNNNNKENCVAEDTSNSKENSKVSDVQKTDYIHVRARRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQKQVEFLSMKLATVNPRLDFNIDDLFEKEVFPTCDANANFQAMGMSTELNSNNPYLQFNSPQQFVPYGGLDAGMNPTDMGLRRSISAPISIPQTFIDSSCFSQQIIPSTTWEGDFQNLYNFNFDQARATSFPSQSQLFT