AlfalfaGEDB | MS.gene063566

M. sativa cultivar XinJiangDaYe / MS.gene063566

KEGG: -
GO: -
PFAM: PF04852.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063566.t1	XP_013467094.1	93.7	189	11	1	1	188	1	189	7.10E-69	270.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063566.t1	Q9LW68	89.6	125	13	0	64	188	48	172	4.2e-62	239.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063566.t1	A0A072VGF5	93.7	189	11	1	1	188	1	189	5.1e-69	270.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050020	MS.gene063566	0.8426	2.34E-58	-1.69E-46
MS.gene050298	MS.gene063566	0.804949	1.69E-49	-1.69E-46
MS.gene050563	MS.gene063566	0.869019	4.24E-66	-1.69E-46
MS.gene050584	MS.gene063566	0.85545	6.26E-62	-1.69E-46
MS.gene050585	MS.gene063566	0.844984	5.39E-59	-1.69E-46
MS.gene050587	MS.gene063566	0.814455	1.53E-51	-1.69E-46
MS.gene05143	MS.gene063566	0.803904	2.79E-49	-1.69E-46
MS.gene051789	MS.gene063566	0.835983	1.22E-56	-1.69E-46
MS.gene052142	MS.gene063566	0.846723	1.82E-59	-1.69E-46
MS.gene053074	MS.gene063566	0.826939	2.06E-54	-1.69E-46
MS.gene053383	MS.gene063566	0.917566	4.54E-86	-1.69E-46
MS.gene05456	MS.gene063566	0.857227	1.88E-62	-1.69E-46
MS.gene055359	MS.gene063566	0.852837	3.55E-61	-1.69E-46
MS.gene058918	MS.gene063566	0.836621	8.41E-57	-1.69E-46
MS.gene059006	MS.gene063566	0.866527	2.67E-65	-1.69E-46
MS.gene059768	MS.gene063566	0.84264	2.29E-58	-1.69E-46
MS.gene060513	MS.gene063566	0.81532	9.82E-52	-1.69E-46
MS.gene060691	MS.gene063566	0.834777	2.46E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063566.t1	MTR_1g442860	97.664	214	4	1	1	213	1	214	3.92e-154	426
MS.gene063566.t1	MTR_1g075990	79.679	187	33	1	19	205	13	194	1.48e-91	267
MS.gene063566.t1	MTR_5g072510	80.282	142	28	0	54	195	27	168	9.57e-88	258
MS.gene063566.t1	MTR_4g094428	83.704	135	22	0	63	197	45	179	9.55e-87	256
MS.gene063566.t1	MTR_7g097030	73.711	194	51	0	1	194	1	194	8.06e-86	253
MS.gene063566.t1	MTR_1g069825	74.227	194	38	1	1	194	1	182	1.85e-84	249
MS.gene063566.t1	MTR_3g031830	71.345	171	37	2	37	195	3	173	1.99e-76	229
MS.gene063566.t1	MTR_2g092950	71.724	145	41	0	43	187	2	146	3.91e-75	224
MS.gene063566.t1	MTR_4g079930	74.436	133	34	0	55	187	20	152	1.23e-74	223
MS.gene063566.t1	MTR_2g076400	77.273	132	30	0	63	194	32	163	4.86e-73	219
MS.gene063566.t1	MTR_2g016110	72.662	139	38	0	56	194	27	165	9.10e-72	216
MS.gene063566.t1	MTR_7g115700	63.804	163	53	3	34	195	1	158	9.32e-71	213
MS.gene063566.t1	MTR_1g080210	84.000	125	20	0	64	188	40	164	2.36e-70	213
MS.gene063566.t1	MTR_8g086350	72.800	125	34	0	63	187	30	154	9.47e-58	181
MS.gene063566.t1	MTR_1g114020	78.431	51	11	0	57	107	64	114	7.77e-23	91.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063566.t1	AT1G07090	77.181	149	29	1	48	196	20	163	6.04e-87	255
MS.gene063566.t1	AT2G31160	84.277	159	24	1	38	195	27	185	1.59e-86	255
MS.gene063566.t1	AT3G23290	95.238	126	6	0	63	188	47	172	6.34e-82	242
MS.gene063566.t1	AT5G58500	80.741	135	25	1	62	195	17	151	9.43e-82	241
MS.gene063566.t1	AT5G28490	76.250	160	32	1	34	193	1	154	7.56e-76	227
MS.gene063566.t1	AT2G42610	68.182	154	43	1	34	187	1	148	6.27e-73	219
MS.gene063566.t1	AT2G42610	68.182	154	43	1	34	187	1	148	6.27e-73	219
MS.gene063566.t1	AT3G04510	69.753	162	49	0	32	193	1	162	1.13e-71	216
MS.gene063566.t1	AT1G78815	62.585	147	55	0	63	209	39	185	1.05e-66	204
MS.gene063566.t1	AT4G18610	69.841	126	37	1	63	187	36	161	8.13e-64	196
MS.gene063566.t1	AT1G16910	64.394	132	47	0	61	192	20	151	9.02e-62	190

Find 48 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCGTTACCTCGACCAATT+TGG	0.269006	1.3:+25602132	MS.gene063566:CDS
TGGATTTGGATGTCCATAAA+AGG	0.277270	1.3:-25602184	None:intergenic
GGACATGGTGCAGGTGGATT+TGG	0.320133	1.3:-25602198	None:intergenic
GACTTTACCTTCGCGAAGTT+AGG	0.323181	1.3:+25602337	MS.gene063566:CDS
GGGTTTGTTATGTTGATCAT+AGG	0.347871	1.3:-25601892	None:intergenic
AAGCCAGAAGCTAATCCTTT+TGG	0.361490	1.3:+25602300	MS.gene063566:CDS
GTGGTGGTCTATGGTTTCTT+AGG	0.368488	1.3:-25602068	None:intergenic
GGTGGTGGTGGTGGCGGCTG+AGG	0.370057	1.3:-25602417	None:intergenic
GTCGTTGTTGATGTTGTTGA+TGG	0.375417	1.3:-25601991	None:intergenic
AAATTCTTGAAGAGAATTCA+TGG	0.377394	1.3:-25601830	None:intergenic
AGACGAGATTGGAACACATT+TGG	0.384522	1.3:+25602039	MS.gene063566:CDS
ACTTTACCTTCGCGAAGTTA+GGG	0.398859	1.3:+25602338	MS.gene063566:CDS
GCACCAAAAGGATTAGCTTC+TGG	0.418214	1.3:-25602303	None:intergenic
GTTGTAGTTGCATTATTTGA+TGG	0.419514	1.3:-25602438	None:intergenic
GGTTTGTTATGTTGATCATA+GGG	0.430093	1.3:-25601891	None:intergenic
GGTAGTCTTGATGCACTCAT+TGG	0.434097	1.3:+25602243	MS.gene063566:CDS
CTTGTCCTCTTCGCCAAGCG+TGG	0.459831	1.3:+25602220	MS.gene063566:CDS
GGTGGTGGTGGCGGCTGAGG+CGG	0.465046	1.3:-25602414	None:intergenic
AGAATCAAAAGAGACGAGAT+TGG	0.470010	1.3:+25602028	MS.gene063566:CDS
GCATTATTTGATGGTGGTGG+TGG	0.471088	1.3:-25602429	None:intergenic
TTATTTGATGGTGGTGGTGG+TGG	0.487231	1.3:-25602426	None:intergenic
TTGTCCTCTTCGCCAAGCGT+GGG	0.488923	1.3:+25602221	MS.gene063566:CDS
CGAGGTAACGCAAGAATTCA+AGG	0.492274	1.3:-25602122	None:intergenic
AGAGTGAAAGTGGTGGTCTA+TGG	0.493036	1.3:-25602077	None:intergenic
GTCATCGTCATCGATGATGA+TGG	0.500415	1.3:-25601913	None:intergenic
GTAGTCTTGATGCACTCATT+GGG	0.507513	1.3:+25602244	MS.gene063566:CDS
GTTGCATTATTTGATGGTGG+TGG	0.513644	1.3:-25602432	None:intergenic
CGAACAGCACGAGCACCAAA+AGG	0.514487	1.3:-25602315	None:intergenic
CACGCTTGGCGAAGAGGACA+AGG	0.527749	1.3:-25602219	None:intergenic
CTTTCACTCTCACGTTGTAG+TGG	0.531680	1.3:+25602090	MS.gene063566:CDS
GGATTTGGATGTCCATAAAA+GGG	0.532805	1.3:-25602183	None:intergenic
TGATGGTGGTGATGTTGTTG+TGG	0.537367	1.3:-25601974	None:intergenic
TGGTGATGTTGTTGTGGACG+AGG	0.542069	1.3:-25601968	None:intergenic
CATCAAGACTACCCCACGCT+TGG	0.543485	1.3:-25602233	None:intergenic
GTAGTTGCATTATTTGATGG+TGG	0.544780	1.3:-25602435	None:intergenic
GTTGTTGATGTTGTTGATGG+TGG	0.556628	1.3:-25601988	None:intergenic
AGAGGACAAGGACATGGTGC+AGG	0.565916	1.3:-25602207	None:intergenic
CTACCCCACGCTTGGCGAAG+AGG	0.567363	1.3:-25602225	None:intergenic
CTACAACGTGAGAGTGAAAG+TGG	0.580478	1.3:-25602087	None:intergenic
GGACAAGGACATGGTGCAGG+TGG	0.588264	1.3:-25602204	None:intergenic
TCATCGTCATCGATGATGAT+GGG	0.604263	1.3:-25601912	None:intergenic
TGTCCTCTTCGCCAAGCGTG+GGG	0.612011	1.3:+25602222	MS.gene063566:CDS
TGTTGTAGATGACGATGGTA+TGG	0.613461	1.3:-25601935	None:intergenic
TGGCGAAGAGGACAAGGACA+TGG	0.629659	1.3:-25602213	None:intergenic
GATTCTCAAGCTAAAGCTAG+AGG	0.638819	1.3:+25602360	MS.gene063566:CDS
GAGAATCCCTAACTTCGCGA+AGG	0.649287	1.3:-25602344	None:intergenic
GCTGATGTTGTAGATGACGA+TGG	0.727039	1.3:-25601940	None:intergenic
CAACGTGAGAGTGAAAGTGG+TGG	0.746060	1.3:-25602084	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTGTAGTTGCATTATTTGA+TGG	-	chr1.3:25602441-25602460	None:intergenic	30.0%
!!	GGTTTGTTATGTTGATCATA+GGG	-	chr1.3:25601894-25601913	None:intergenic	30.0%
!!	TTTTGATTCTCGTATCTACT+TGG	-	chr1.3:25602018-25602037	None:intergenic	30.0%
!!!	GAACCTTTGTTTTTCCAAAT+TGG	-	chr1.3:25602149-25602168	None:intergenic	30.0%
!!!	GTTCATCATGTCTTTGTTTT+TGG	-	chr1.3:25601866-25601885	None:intergenic	30.0%
	AGAATCAAAAGAGACGAGAT+TGG	+	chr1.3:25602028-25602047	MS.gene063566:CDS	35.0%
	CGACCAATTTGGAAAAACAA+AGG	+	chr1.3:25602143-25602162	MS.gene063566:CDS	35.0%
	GGATTTGGATGTCCATAAAA+GGG	-	chr1.3:25602186-25602205	None:intergenic	35.0%
	GTAGTTGCATTATTTGATGG+TGG	-	chr1.3:25602438-25602457	None:intergenic	35.0%
	TGGATTTGGATGTCCATAAA+AGG	-	chr1.3:25602187-25602206	None:intergenic	35.0%
!	ACTCAGATTTGTCCCTTTTA+TGG	+	chr1.3:25602171-25602190	MS.gene063566:CDS	35.0%
!!	GGGTTTGTTATGTTGATCAT+AGG	-	chr1.3:25601895-25601914	None:intergenic	35.0%
!!	TTGTTTTTCCAAATTGGTCG+AGG	-	chr1.3:25602143-25602162	None:intergenic	35.0%
	ACTTTACCTTCGCGAAGTTA+GGG	+	chr1.3:25602338-25602357	MS.gene063566:CDS	40.0%
	AGACGAGATTGGAACACATT+TGG	+	chr1.3:25602039-25602058	MS.gene063566:CDS	40.0%
	GATTCTCAAGCTAAAGCTAG+AGG	+	chr1.3:25602360-25602379	MS.gene063566:CDS	40.0%
	GTCGTTGTTGATGTTGTTGA+TGG	-	chr1.3:25601994-25602013	None:intergenic	40.0%
	TCATCGTCATCGATGATGAT+GGG	-	chr1.3:25601915-25601934	None:intergenic	40.0%
	TGTTGTAGATGACGATGGTA+TGG	-	chr1.3:25601938-25601957	None:intergenic	40.0%
!	AAGCCAGAAGCTAATCCTTT+TGG	+	chr1.3:25602300-25602319	MS.gene063566:CDS	40.0%
!	GTAGTCTTGATGCACTCATT+GGG	+	chr1.3:25602244-25602263	MS.gene063566:CDS	40.0%
!	GTTGTTGATGTTGTTGATGG+TGG	-	chr1.3:25601991-25602010	None:intergenic	40.0%
!!	CTGCTTTTGAAGAGAATGGT+GGG	+	chr1.3:25602277-25602296	MS.gene063566:CDS	40.0%
!!	GTTGCATTATTTGATGGTGG+TGG	-	chr1.3:25602435-25602454	None:intergenic	40.0%
!!!	AGAGCTGCTTTTGAAGAGAA+TGG	+	chr1.3:25602273-25602292	MS.gene063566:CDS	40.0%
	AGAGTGAAAGTGGTGGTCTA+TGG	-	chr1.3:25602080-25602099	None:intergenic	45.0%
	CGAGGTAACGCAAGAATTCA+AGG	-	chr1.3:25602125-25602144	None:intergenic	45.0%
	CTACAACGTGAGAGTGAAAG+TGG	-	chr1.3:25602090-25602109	None:intergenic	45.0%
	CTTTCACTCTCACGTTGTAG+TGG	+	chr1.3:25602090-25602109	MS.gene063566:CDS	45.0%
	GACTTTACCTTCGCGAAGTT+AGG	+	chr1.3:25602337-25602356	MS.gene063566:CDS	45.0%
	GCTGATGTTGTAGATGACGA+TGG	-	chr1.3:25601943-25601962	None:intergenic	45.0%
	GTCATCGTCATCGATGATGA+TGG	-	chr1.3:25601916-25601935	None:intergenic	45.0%
	GTGGTGGTCTATGGTTTCTT+AGG	-	chr1.3:25602071-25602090	None:intergenic	45.0%
	TTGCGTTACCTCGACCAATT+TGG	+	chr1.3:25602132-25602151	MS.gene063566:CDS	45.0%
!	GGTAGTCTTGATGCACTCAT+TGG	+	chr1.3:25602243-25602262	MS.gene063566:CDS	45.0%
!!	GCACCAAAAGGATTAGCTTC+TGG	-	chr1.3:25602306-25602325	None:intergenic	45.0%
!!	GCATTATTTGATGGTGGTGG+TGG	-	chr1.3:25602432-25602451	None:intergenic	45.0%
!!	TGATGGTGGTGATGTTGTTG+TGG	-	chr1.3:25601977-25601996	None:intergenic	45.0%
!!	TTATTTGATGGTGGTGGTGG+TGG	-	chr1.3:25602429-25602448	None:intergenic	45.0%
!!!	GCTGCTTTTGAAGAGAATGG+TGG	+	chr1.3:25602276-25602295	MS.gene063566:CDS	45.0%
	CAACGTGAGAGTGAAAGTGG+TGG	-	chr1.3:25602087-25602106	None:intergenic	50.0%
	GAGAATCCCTAACTTCGCGA+AGG	-	chr1.3:25602347-25602366	None:intergenic	50.0%
!	TGGTGATGTTGTTGTGGACG+AGG	-	chr1.3:25601971-25601990	None:intergenic	50.0%
	AGAGGACAAGGACATGGTGC+AGG	-	chr1.3:25602210-25602229	None:intergenic	55.0%
	CATCAAGACTACCCCACGCT+TGG	-	chr1.3:25602236-25602255	None:intergenic	55.0%
	CGAACAGCACGAGCACCAAA+AGG	-	chr1.3:25602318-25602337	None:intergenic	55.0%
	TGGCGAAGAGGACAAGGACA+TGG	-	chr1.3:25602216-25602235	None:intergenic	55.0%
	TTGTCCTCTTCGCCAAGCGT+GGG	+	chr1.3:25602221-25602240	MS.gene063566:CDS	55.0%
!	GGACATGGTGCAGGTGGATT+TGG	-	chr1.3:25602201-25602220	None:intergenic	55.0%
!!	TTTGATGGTGGTGGTGGTGG+CGG	-	chr1.3:25602426-25602445	None:intergenic	55.0%
	CACGCTTGGCGAAGAGGACA+AGG	-	chr1.3:25602222-25602241	None:intergenic	60.0%
	CTTGTCCTCTTCGCCAAGCG+TGG	+	chr1.3:25602220-25602239	MS.gene063566:CDS	60.0%
	GGACAAGGACATGGTGCAGG+TGG	-	chr1.3:25602207-25602226	None:intergenic	60.0%
	TGTCCTCTTCGCCAAGCGTG+GGG	+	chr1.3:25602222-25602241	MS.gene063566:CDS	60.0%
	CTACCCCACGCTTGGCGAAG+AGG	-	chr1.3:25602228-25602247	None:intergenic	65.0%
!!	GGTGGTGGTGGCGGCTGAGG+CGG	-	chr1.3:25602417-25602436	None:intergenic	75.0%
!!	GGTGGTGGTGGTGGCGGCTG+AGG	-	chr1.3:25602420-25602439	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	25601832	25602473	25601832	ID=MS.gene063566
chr1.3	mRNA	25601832	25602473	25601832	ID=MS.gene063566.t1;Parent=MS.gene063566
chr1.3	exon	25601832	25602473	25601832	ID=MS.gene063566.t1.exon1;Parent=MS.gene063566.t1
chr1.3	CDS	25601832	25602473	25601832	ID=cds.MS.gene063566.t1;Parent=MS.gene063566.t1

Gene Sequence

>MS.gene063566

ATGAATTCTCTTCAAGAATTTGAATCATCAACCAAAAACAAAGACATGATGAACACAAACCCTATGATCAACATAACAAACCCATCATCATCGATGACGATGACCATACCATCGTCATCTACAACATCAGCATCATCCTCGTCCACAACAACATCACCACCATCAACAACATCAACAACGACACCAAGTAGATACGAGAATCAAAAGAGACGAGATTGGAACACATTTGGACAATACCTAAGAAACCATAGACCACCACTTTCACTCTCACGTTGTAGTGGAGCTCATGTCCTTGAATTCTTGCGTTACCTCGACCAATTTGGAAAAACAAAGGTTCACACTCAGATTTGTCCCTTTTATGGACATCCAAATCCACCTGCACCATGTCCTTGTCCTCTTCGCCAAGCGTGGGGTAGTCTTGATGCACTCATTGGGAGACTTAGAGCTGCTTTTGAAGAGAATGGTGGGAAGCCAGAAGCTAATCCTTTTGGTGCTCGTGCTGTTCGACTTTACCTTCGCGAAGTTAGGGATTCTCAAGCTAAAGCTAGAGGTATTAGTTATGAGAAAAAGAAGCGCAAGCGTCCGCCTCAGCCGCCACCACCACCACCATCAAATAATGCAACTACAACTGATCTTCACTAG

Protein sequence

>MS.gene063566.t1

MNSLQEFESSTKNKDMMNTNPMINITNPSSSMTMTIPSSSTTSASSSSTTTSPPSTTSTTTPSRYENQKRRDWNTFGQYLRNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHTQICPFYGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPEANPFGARAVRLYLREVRDSQAKARGISYEKKKRKRPPQPPPPPPSNNATTTDLH