AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064162


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064162.t1 MTR_4g123700 95.349 301 13 1 1 301 1 300 0.0 553
MS.gene064162.t1 MTR_4g105550 35.374 294 162 10 2 280 10 290 6.32e-51 170
MS.gene064162.t1 MTR_4g132450 32.443 262 149 13 34 277 38 289 5.66e-28 110
MS.gene064162.t1 MTR_5g037310 29.592 294 184 10 13 288 8 296 1.03e-27 109
MS.gene064162.t1 MTR_5g037310 29.592 294 184 10 13 288 8 296 1.26e-27 109
MS.gene064162.t1 MTR_2g023970 33.203 256 135 13 32 265 22 263 1.45e-27 109
MS.gene064162.t1 MTR_8g090055 30.712 267 156 11 15 265 10 263 2.02e-27 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064162.t1 AT3G17350 63.934 244 79 4 32 267 29 271 5.50e-112 326
MS.gene064162.t1 AT3G17350 64.583 240 76 4 34 265 31 269 5.60e-111 323
MS.gene064162.t1 AT5G50290 33.582 268 160 7 13 264 1 266 6.47e-47 160
MS.gene064162.t1 AT1G10380 34.800 250 136 9 34 264 29 270 4.62e-32 121
MS.gene064162.t1 AT1G10380 34.800 250 136 9 34 264 29 270 5.13e-32 120
MS.gene064162.t1 AT1G11915 30.827 266 163 8 5 255 4 263 2.86e-29 114

Find 59 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCCTTTGGATTGGGTTTA+TGG 0.099494 8.4:+11096150 MS.gene064162:CDS
GTTTATGAGTATGAATATTT+TGG 0.176981 8.4:+11096077 MS.gene064162:CDS
GTCCTTTGGATTGGGTTTAT+GGG 0.234143 8.4:+11096151 MS.gene064162:CDS
TTATTCTTTCAAACTCCTTC+TGG 0.239774 8.4:+11095721 MS.gene064162:CDS
AAACCGAATGGGTAGTTTAT+AGG 0.261528 8.4:-11095640 None:intergenic
CACACTTGTGATGAGCTTTA+TGG 0.305918 8.4:+11095970 MS.gene064162:CDS
ATTGAGAGGTGTTGGTCCTT+TGG 0.340253 8.4:+11096137 MS.gene064162:CDS
TCCTTCTGATATCATGCTTC+CGG 0.381010 8.4:-11096788 None:intergenic
CAACTTTCACATCCAGTATC+AGG 0.395675 8.4:-11096195 None:intergenic
AAGGAGTGTACAATGCTCTT+TGG 0.408970 8.4:+11096806 MS.gene064162:CDS
CACAATCTGGTGGGATTTGT+GGG 0.413624 8.4:+11096220 MS.gene064162:CDS
TGGCTTGTAGTAGTTGTAGA+TGG 0.416349 8.4:-11095570 None:intergenic
TAAAACAAAGATATTTGTAA+TGG 0.422370 8.4:-11095936 None:intergenic
AGGTGTTGGTCCTTTGGATT+GGG 0.424565 8.4:+11096143 MS.gene064162:CDS
GAGGTGTTGGTCCTTTGGAT+TGG 0.424668 8.4:+11096142 MS.gene064162:CDS
TGTATCTCAGCTGCTGGAAC+CGG 0.428657 8.4:+11096769 MS.gene064162:intron
ATGGAGCAAGTTGACATTGA+TGG 0.431134 8.4:-11095799 None:intergenic
TATAAACTACCCATTCGGTT+TGG 0.432975 8.4:+11095642 MS.gene064162:CDS
TACCCATTCGGTTTGGAAGA+TGG 0.440399 8.4:+11095649 MS.gene064162:CDS
TATTTGTAATGGTTAAGAAC+AGG 0.444608 8.4:-11095925 None:intergenic
ACACAATCTGGTGGGATTTG+TGG 0.447706 8.4:+11096219 MS.gene064162:CDS
TTCGGTTTGGAAGATGGTTG+TGG 0.449681 8.4:+11095655 MS.gene064162:CDS
GCAGGGTGAGAGTGTGTGAA+TGG 0.463159 8.4:-11095596 None:intergenic
ATTCCTATAAACTACCCATT+CGG 0.492935 8.4:+11095637 MS.gene064162:CDS
ATCATGATGAGGTTGAAGAA+TGG 0.498307 8.4:-11095818 None:intergenic
TGCTATTATTCCTCCATCAC+AGG 0.508874 8.4:+11095864 MS.gene064162:CDS
AACCATCTTCCAAACCGAAT+GGG 0.514516 8.4:-11095651 None:intergenic
TGGAGCAAGTTGACATTGAT+GGG 0.527863 8.4:-11095798 None:intergenic
TGTGAAAGTTGTACACAATC+TGG 0.535884 8.4:+11096207 MS.gene064162:CDS
AGTGATAAATTGAGAGGTGT+TGG 0.537265 8.4:+11096129 MS.gene064162:CDS
ATGAGTTTGAAGATTGTGAA+TGG 0.557897 8.4:-11095535 None:intergenic
CAGGGTGAGAGTGTGTGAAT+GGG 0.561055 8.4:-11095595 None:intergenic
CAAGTTTCACTAATTCAACA+AGG 0.561620 8.4:+11096280 MS.gene064162:CDS
TGAGAGTGTGTGAATGGGAA+TGG 0.572032 8.4:-11095590 None:intergenic
ACTAATTCAACAAGGGAATG+TGG 0.573682 8.4:+11096288 MS.gene064162:CDS
TATAGGAATGGTACCACATG+AGG 0.575471 8.4:-11095623 None:intergenic
CAACCATCTTCCAAACCGAA+TGG 0.581667 8.4:-11095652 None:intergenic
CTACAATAAGCAAAACCACT+TGG 0.586476 8.4:-11096837 None:intergenic
ATCCCATAAACCCAATCCAA+AGG 0.604071 8.4:-11096153 None:intergenic
ACCGGAAGCATGATATCAGA+AGG 0.604148 8.4:+11096787 MS.gene064162:CDS
AAAACAGTGTCCTGTGATGG+AGG 0.608306 8.4:-11095874 None:intergenic
TGTACAATGCTCTTTGGACA+CGG 0.610218 8.4:+11096812 MS.gene064162:CDS
CTCGTGAAACAACAAGGTGG+TGG 0.615967 8.4:-11096039 None:intergenic
GAATGGGTAGTTTATAGGAA+TGG 0.619390 8.4:-11095635 None:intergenic
TGAACTTTGTAAGAACCAGA+AGG 0.628532 8.4:-11095736 None:intergenic
TCACCCTGCAAAACCTCATG+TGG 0.633355 8.4:+11095610 MS.gene064162:CDS
TTTAATAGTGATAAATTGAG+AGG 0.647028 8.4:+11096123 MS.gene064162:CDS
TTGAACATGTTTCTGAACTG+TGG 0.654643 8.4:-11095682 None:intergenic
TTATAGCTCGTGAAACAACA+AGG 0.671730 8.4:-11096045 None:intergenic
TCTTTGGACACGGCACCAAG+TGG 0.673512 8.4:+11096822 MS.gene064162:CDS
TGATGGGTCGTAGAGTACCA+TGG 0.674328 8.4:-11095782 None:intergenic
ACTTGTGATGAGCTTTATGG+TGG 0.679797 8.4:+11095973 MS.gene064162:CDS
GGGTTTGATACTGAAACTGA+AGG 0.680082 8.4:+11096240 MS.gene064162:CDS
AAGTTTCACTAATTCAACAA+GGG 0.686221 8.4:+11096281 MS.gene064162:CDS
GAAAGTTGTACACAATCTGG+TGG 0.691833 8.4:+11096210 MS.gene064162:CDS
GTCATGAGAAAATCATGATG+AGG 0.693658 8.4:-11095829 None:intergenic
AAAGTTGTACACAATCTGGT+GGG 0.724462 8.4:+11096211 MS.gene064162:CDS
AGTTTGAAAGAATAACTCAG+TGG 0.732725 8.4:-11095713 None:intergenic
TAGCTCGTGAAACAACAAGG+TGG 0.804087 8.4:-11096042 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTTTAGAAATTAAAA+AGG + chr8.4:11096446-11096465 MS.gene064162:intron 10.0%
!! TAAAACAAAGATATTTGTAA+TGG - chr8.4:11095939-11095958 None:intergenic 15.0%
!! TTTAATAGTGATAAATTGAG+AGG + chr8.4:11096123-11096142 MS.gene064162:CDS 20.0%
!!! GTTTATGAGTATGAATATTT+TGG + chr8.4:11096077-11096096 MS.gene064162:CDS 20.0%
!!! TATCTTTGTTTTAACTTTGA+AGG + chr8.4:11095946-11095965 MS.gene064162:CDS 20.0%
!!! TATTTGGATTTTGAATCATT+TGG + chr8.4:11096331-11096350 MS.gene064162:intron 20.0%
! AAATAGAATACGGAAACTTA+CGG - chr8.4:11096749-11096768 None:intergenic 25.0%
! AAGTTTCACTAATTCAACAA+GGG + chr8.4:11096281-11096300 MS.gene064162:CDS 25.0%
! CTGAGATACAAAATAGAATA+CGG - chr8.4:11096759-11096778 None:intergenic 25.0%
! TATTTGTAATGGTTAAGAAC+AGG - chr8.4:11095928-11095947 None:intergenic 25.0%
!!! CTTTTTTAGTTGATAACAGT+AGG + chr8.4:11096613-11096632 MS.gene064162:intron 25.0%
!!! TAAGTTTTATCTGAGTTGTT+AGG + chr8.4:11096641-11096660 MS.gene064162:intron 25.0%
AGTTTGAAAGAATAACTCAG+TGG - chr8.4:11095716-11095735 None:intergenic 30.0%
ATGAGTTTGAAGATTGTGAA+TGG - chr8.4:11095538-11095557 None:intergenic 30.0%
ATTCCTATAAACTACCCATT+CGG + chr8.4:11095637-11095656 MS.gene064162:CDS 30.0%
CAAGTTTCACTAATTCAACA+AGG + chr8.4:11096280-11096299 MS.gene064162:CDS 30.0%
CTACTACTTGGTTTCTTATT+TGG + chr8.4:11096315-11096334 MS.gene064162:intron 30.0%
GGTGCTAGTTAATATGAATA+GGG + chr8.4:11096352-11096371 MS.gene064162:intron 30.0%
TGACTACAACAAAAAAACCA+TGG + chr8.4:11095765-11095784 MS.gene064162:CDS 30.0%
TTATTCTTTCAAACTCCTTC+TGG + chr8.4:11095721-11095740 MS.gene064162:CDS 30.0%
! AGTTTTTCTTCACAAGATGA+GGG + chr8.4:11096858-11096877 MS.gene064162:CDS 30.0%
! TAGTTTTTCTTCACAAGATG+AGG + chr8.4:11096857-11096876 MS.gene064162:CDS 30.0%
! TCACCAACAACAATTTTGAA+AGG - chr8.4:11096581-11096600 None:intergenic 30.0%
! TGGTGCTAGTTAATATGAAT+AGG + chr8.4:11096351-11096370 MS.gene064162:intron 30.0%
!! CACCAACAACAATTTTGAAA+GGG - chr8.4:11096580-11096599 None:intergenic 30.0%
!!! GGTTTTTTTGTTGTAGTCAA+TGG - chr8.4:11095764-11095783 None:intergenic 30.0%
AAACCGAATGGGTAGTTTAT+AGG - chr8.4:11095643-11095662 None:intergenic 35.0%
AAAGTTGTACACAATCTGGT+GGG + chr8.4:11096211-11096230 MS.gene064162:CDS 35.0%
ACTAATTCAACAAGGGAATG+TGG + chr8.4:11096288-11096307 MS.gene064162:CDS 35.0%
ATCATGATGAGGTTGAAGAA+TGG - chr8.4:11095821-11095840 None:intergenic 35.0%
CACCCTTTCAAAATTGTTGT+TGG + chr8.4:11096575-11096594 MS.gene064162:intron 35.0%
CTACAATAAGCAAAACCACT+TGG - chr8.4:11096840-11096859 None:intergenic 35.0%
GAAAATCATACAGCACTAAC+TGG + chr8.4:11096468-11096487 MS.gene064162:intron 35.0%
GAATGGGTAGTTTATAGGAA+TGG - chr8.4:11095638-11095657 None:intergenic 35.0%
GTCATGAGAAAATCATGATG+AGG - chr8.4:11095832-11095851 None:intergenic 35.0%
TATAAACTACCCATTCGGTT+TGG + chr8.4:11095642-11095661 MS.gene064162:CDS 35.0%
TGAACTTTGTAAGAACCAGA+AGG - chr8.4:11095739-11095758 None:intergenic 35.0%
TGTGAAAGTTGTACACAATC+TGG + chr8.4:11096207-11096226 MS.gene064162:CDS 35.0%
TTATAGCTCGTGAAACAACA+AGG - chr8.4:11096048-11096067 None:intergenic 35.0%
! AGTGATAAATTGAGAGGTGT+TGG + chr8.4:11096129-11096148 MS.gene064162:CDS 35.0%
! GTTTTTCTTCACAAGATGAG+GGG + chr8.4:11096859-11096878 MS.gene064162:CDS 35.0%
! TTGAACATGTTTCTGAACTG+TGG - chr8.4:11095685-11095704 None:intergenic 35.0%
! TTTTTCTTCACAAGATGAGG+GGG + chr8.4:11096860-11096879 MS.gene064162:CDS 35.0%
AACCATCTTCCAAACCGAAT+GGG - chr8.4:11095654-11095673 None:intergenic 40.0%
ACAAAAACAGTGTCCTGTGA+TGG - chr8.4:11095880-11095899 None:intergenic 40.0%
ATCCCATAAACCCAATCCAA+AGG - chr8.4:11096156-11096175 None:intergenic 40.0%
CAACTTTCACATCCAGTATC+AGG - chr8.4:11096198-11096217 None:intergenic 40.0%
CACACTTGTGATGAGCTTTA+TGG + chr8.4:11095970-11095989 MS.gene064162:CDS 40.0%
GAAAGTTGTACACAATCTGG+TGG + chr8.4:11096210-11096229 MS.gene064162:CDS 40.0%
GAATACGGAAACTTACGGTT+AGG - chr8.4:11096744-11096763 None:intergenic 40.0%
GAATGTGGTAAGCTACTACT+TGG + chr8.4:11096303-11096322 MS.gene064162:intron 40.0%
GGGTTTGATACTGAAACTGA+AGG + chr8.4:11096240-11096259 MS.gene064162:CDS 40.0%
TATAGGAATGGTACCACATG+AGG - chr8.4:11095626-11095645 None:intergenic 40.0%
TCCTTCTGATATCATGCTTC+CGG - chr8.4:11096791-11096810 None:intergenic 40.0%
TGCTATTATTCCTCCATCAC+AGG + chr8.4:11095864-11095883 MS.gene064162:CDS 40.0%
TGTACAATGCTCTTTGGACA+CGG + chr8.4:11096812-11096831 MS.gene064162:CDS 40.0%
TTGCTTCGAGCTTAGAGTTA+AGG - chr8.4:11096701-11096720 None:intergenic 40.0%
! AAGGAGTGTACAATGCTCTT+TGG + chr8.4:11096806-11096825 MS.gene064162:CDS 40.0%
! ACTTGTGATGAGCTTTATGG+TGG + chr8.4:11095973-11095992 MS.gene064162:CDS 40.0%
! ATGGAGCAAGTTGACATTGA+TGG - chr8.4:11095802-11095821 None:intergenic 40.0%
! ATGGTGGTTGCAATGCTTTT+AGG + chr8.4:11095989-11096008 MS.gene064162:CDS 40.0%
! CTATTTTGTATCTCAGCTGC+TGG + chr8.4:11096763-11096782 MS.gene064162:intron 40.0%
! GTCCTTTGGATTGGGTTTAT+GGG + chr8.4:11096151-11096170 MS.gene064162:CDS 40.0%
! TCTTTTAGTGTGCCTGATAC+TGG + chr8.4:11096183-11096202 MS.gene064162:CDS 40.0%
! TGGAGCAAGTTGACATTGAT+GGG - chr8.4:11095801-11095820 None:intergenic 40.0%
! TGGCTTGTAGTAGTTGTAGA+TGG - chr8.4:11095573-11095592 None:intergenic 40.0%
! TGGTGGTTGCAATGCTTTTA+GGG + chr8.4:11095990-11096009 MS.gene064162:CDS 40.0%
AAAACAGTGTCCTGTGATGG+AGG - chr8.4:11095877-11095896 None:intergenic 45.0%
ACACAATCTGGTGGGATTTG+TGG + chr8.4:11096219-11096238 MS.gene064162:CDS 45.0%
CAACCATCTTCCAAACCGAA+TGG - chr8.4:11095655-11095674 None:intergenic 45.0%
CACAATCTGGTGGGATTTGT+GGG + chr8.4:11096220-11096239 MS.gene064162:CDS 45.0%
GCAATTGAGCTACCAAAACC+TGG + chr8.4:11096717-11096736 MS.gene064162:intron 45.0%
TAGCTCGTGAAACAACAAGG+TGG - chr8.4:11096045-11096064 None:intergenic 45.0%
! ACCGGAAGCATGATATCAGA+AGG + chr8.4:11096787-11096806 MS.gene064162:CDS 45.0%
! GGTCCTTTGGATTGGGTTTA+TGG + chr8.4:11096150-11096169 MS.gene064162:CDS 45.0%
! GTACCACATGAGGTTTTGCA+GGG - chr8.4:11095616-11095635 None:intergenic 45.0%
! TGAGAGTGTGTGAATGGGAA+TGG - chr8.4:11095593-11095612 None:intergenic 45.0%
! TTACGGTTAGGACCAGGTTT+TGG - chr8.4:11096732-11096751 None:intergenic 45.0%
!! AGGTGTTGGTCCTTTGGATT+GGG + chr8.4:11096143-11096162 MS.gene064162:CDS 45.0%
!! ATTGAGAGGTGTTGGTCCTT+TGG + chr8.4:11096137-11096156 MS.gene064162:CDS 45.0%
!! TACCCATTCGGTTTGGAAGA+TGG + chr8.4:11095649-11095668 MS.gene064162:CDS 45.0%
!! TTCGGTTTGGAAGATGGTTG+TGG + chr8.4:11095655-11095674 MS.gene064162:CDS 45.0%
CTCGTGAAACAACAAGGTGG+TGG - chr8.4:11096042-11096061 None:intergenic 50.0%
GGAAACTTACGGTTAGGACC+AGG - chr8.4:11096738-11096757 None:intergenic 50.0%
TCACCCTGCAAAACCTCATG+TGG + chr8.4:11095610-11095629 MS.gene064162:CDS 50.0%
TGTATCTCAGCTGCTGGAAC+CGG + chr8.4:11096769-11096788 MS.gene064162:intron 50.0%
! CAGGGTGAGAGTGTGTGAAT+GGG - chr8.4:11095598-11095617 None:intergenic 50.0%
! GGTACCACATGAGGTTTTGC+AGG - chr8.4:11095617-11095636 None:intergenic 50.0%
! TGATGGGTCGTAGAGTACCA+TGG - chr8.4:11095785-11095804 None:intergenic 50.0%
!! GAGGTGTTGGTCCTTTGGAT+TGG + chr8.4:11096142-11096161 MS.gene064162:CDS 50.0%
TCTTTGGACACGGCACCAAG+TGG + chr8.4:11096822-11096841 MS.gene064162:CDS 55.0%
! GCAGGGTGAGAGTGTGTGAA+TGG - chr8.4:11095599-11095618 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 11095517 11096891 11095517 ID=MS.gene064162
chr8.4 mRNA 11095517 11096891 11095517 ID=MS.gene064162.t1;Parent=MS.gene064162
chr8.4 exon 11095517 11096309 11095517 ID=MS.gene064162.t1.exon1;Parent=MS.gene064162.t1
chr8.4 CDS 11095517 11096309 11095517 ID=cds.MS.gene064162.t1;Parent=MS.gene064162.t1
chr8.4 exon 11096779 11096891 11096779 ID=MS.gene064162.t1.exon2;Parent=MS.gene064162.t1
chr8.4 CDS 11096779 11096891 11096779 ID=cds.MS.gene064162.t1;Parent=MS.gene064162.t1
Gene Sequence

>MS.gene064162

ATGCATCATCTTCTAACACCATTCACAATCTTCAAACTCATTATACTAACACTCCATCTACAACTACTACAAGCCATTCCCATTCACACACTCTCACCCTGCAAAACCTCATGTGGTACCATTCCTATAAACTACCCATTCGGTTTGGAAGATGGTTGTGGAGCACCACAGTTCAGAAACATGTTCAACTGCACAACCACTGAGTTATTCTTTCAAACTCCTTCTGGTTCTTACAAAGTTCAATCCATTGACTACAACAAAAAAACCATGGTACTCTACGACCCATCAATGTCAACTTGCTCCATTCTTCAACCTCATCATGATTTTCTCATGACAGATATTCAATCTGCTATTATTCCTCCATCACAGGACACTGTTTTTGTTCTGTTAAATTGCTCTATAGACTCTCCTGTTCTTAACCATTACAAATATCTTTGTTTTAACTTTGAAGGACACACTTGTGATGAGCTTTATGGTGGTTGCAATGCTTTTAGGGTGTTTCATTTGATGACAAATAGCTCACCACCACCTTGTTGTTTCACGAGCTATAATACTGTGAAGTTTATGAGTATGAATATTTTGGATTGTACACATTATACTAGTGTGTTTAATAGTGATAAATTGAGAGGTGTTGGTCCTTTGGATTGGGTTTATGGGATTAAGCTTTCTTTTAGTGTGCCTGATACTGGATGTGAAAGTTGTACACAATCTGGTGGGATTTGTGGGTTTGATACTGAAACTGAAGGTCTTCTTTGTCTCTGCTCAAGTTTCACTAATTCAACAAGGGAATGTGGTAAGCTACTACTTGGTTTCTTATTTGGATTTTGAATCATTTGGTGCTAGTTAATATGAATAGGGTTGTATAGCACACTAATTTGAAAGAAAAAAATAAGAAATTGTTTTTCAAAACTAGTTAATCAATTTTTCTTTTCTTTTTTAGAAATTAAAAAGGAAAATCATACAGCACTAACTGGTTTTAGAGTGAAATGTTAGTATATTATAGATGCAAGAACTAAACTTAGACGCATTTTGTTTTCTTTCAAAAGACAACTTTTTTACACCCTTTCAAAATTGTTGTTGGTGACAATGATACTATCTTTTTTAGTTGATAACAGTAGGAATACTAAGTTTTATCTGAGTTGTTAGGTTAATATTTTCTTCTTTATCAACTCTTTCAACATCCTTAACTCTAAGCTCGAAGCAATTGAGCTACCAAAACCTGGTCCTAACCGTAAGTTTCCGTATTCTATTTTGTATCTCAGCTGCTGGAACCGGAAGCATGATATCAGAAGGAGTGTACAATGCTCTTTGGACACGGCACCAAGTGGTTTTGCTTATTGTAGTTTTTCTTCACAAGATGAGGGGGCTAATTTGA

Protein sequence

>MS.gene064162.t1

MHHLLTPFTIFKLIILTLHLQLLQAIPIHTLSPCKTSCGTIPINYPFGLEDGCGAPQFRNMFNCTTTELFFQTPSGSYKVQSIDYNKKTMVLYDPSMSTCSILQPHHDFLMTDIQSAIIPPSQDTVFVLLNCSIDSPVLNHYKYLCFNFEGHTCDELYGGCNAFRVFHLMTNSSPPPCCFTSYNTVKFMSMNILDCTHYTSVFNSDKLRGVGPLDWVYGIKLSFSVPDTGCESCTQSGGICGFDTETEGLLCLCSSFTNSTRECAAGTGSMISEGVYNALWTRHQVVLLIVVFLHKMRGLI