Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064199.t1 | RYR68903.1 | 79.3 | 1064 | 215 | 3 | 1 | 1062 | 1 | 1061 | 0.00E+00 | 1751.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064199.t1 | M1BYJ7 | 68.5 | 546 | 169 | 2 | 7 | 549 | 4 | 549 | 2.0e-227 | 790.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064199.t1 | A0A445E0D3 | 79.3 | 1064 | 215 | 3 | 1 | 1062 | 1 | 1061 | 0.0e+00 | 1751.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene02306 | MS.gene064199 | PPI |
| MS.gene064199 | MS.gene048610 | PPI |
| MS.gene064199 | MS.gene052562 | PPI |
| MS.gene32211 | MS.gene064199 | PPI |
| MS.gene064199 | MS.gene009833 | PPI |
| MS.gene048480 | MS.gene064199 | PPI |
| MS.gene064199 | MS.gene57021 | PPI |
| MS.gene01745 | MS.gene064199 | PPI |
| MS.gene064199 | MS.gene53986 | PPI |
| MS.gene50180 | MS.gene064199 | PPI |
| MS.gene064199 | MS.gene03971 | PPI |
| MS.gene064199 | MS.gene066720 | PPI |
| MS.gene01336 | MS.gene064199 | PPI |
| MS.gene064199 | MS.gene002362 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064199.t1 | MTR_4g045997 | 97.268 | 549 | 15 | 0 | 1 | 549 | 1 | 549 | 0.0 | 1114 |
| MS.gene064199.t1 | MTR_4g045997 | 59.961 | 517 | 203 | 3 | 549 | 1062 | 30 | 545 | 0.0 | 657 |
| MS.gene064199.t1 | MTR_4g045997 | 97.268 | 549 | 15 | 0 | 1 | 549 | 1 | 549 | 0.0 | 1114 |
| MS.gene064199.t1 | MTR_4g045997 | 59.961 | 517 | 203 | 3 | 549 | 1062 | 30 | 545 | 0.0 | 657 |
| MS.gene064199.t1 | MTR_4g045993 | 96.693 | 514 | 10 | 1 | 549 | 1062 | 31 | 537 | 0.0 | 1029 |
| MS.gene064199.t1 | MTR_4g045993 | 58.377 | 567 | 222 | 5 | 1 | 565 | 1 | 555 | 0.0 | 687 |
| MS.gene064199.t1 | MTR_2g011730 | 68.533 | 518 | 157 | 3 | 549 | 1062 | 31 | 546 | 0.0 | 737 |
| MS.gene064199.t1 | MTR_2g011730 | 64.407 | 531 | 179 | 4 | 24 | 549 | 25 | 550 | 0.0 | 716 |
| MS.gene064199.t1 | MTR_4g091240 | 68.015 | 519 | 158 | 4 | 549 | 1062 | 31 | 546 | 0.0 | 736 |
| MS.gene064199.t1 | MTR_4g091240 | 61.273 | 550 | 202 | 5 | 6 | 549 | 6 | 550 | 0.0 | 707 |
| MS.gene064199.t1 | MTR_4g091240 | 69.159 | 428 | 128 | 2 | 639 | 1062 | 15 | 442 | 0.0 | 622 |
| MS.gene064199.t1 | MTR_4g091240 | 63.170 | 429 | 153 | 3 | 126 | 549 | 18 | 446 | 0.0 | 582 |
| MS.gene064199.t1 | MTR_4g090580 | 37.903 | 372 | 202 | 10 | 43 | 406 | 99 | 449 | 9.80e-65 | 229 |
| MS.gene064199.t1 | MTR_4g090580 | 34.400 | 375 | 208 | 10 | 563 | 913 | 95 | 455 | 3.63e-62 | 222 |
| MS.gene064199.t1 | MTR_5g071250 | 42.236 | 322 | 171 | 7 | 56 | 372 | 64 | 375 | 1.86e-63 | 222 |
| MS.gene064199.t1 | MTR_5g071250 | 39.881 | 336 | 180 | 8 | 563 | 889 | 53 | 375 | 3.99e-59 | 209 |
| MS.gene064199.t1 | MTR_5g071250 | 42.236 | 322 | 171 | 7 | 56 | 372 | 64 | 375 | 6.56e-63 | 223 |
| MS.gene064199.t1 | MTR_5g071250 | 39.881 | 336 | 180 | 8 | 563 | 889 | 53 | 375 | 2.29e-58 | 210 |
| MS.gene064199.t1 | MTR_3g464380 | 40.683 | 322 | 176 | 7 | 56 | 372 | 67 | 378 | 4.15e-60 | 215 |
| MS.gene064199.t1 | MTR_3g464380 | 37.883 | 359 | 196 | 10 | 569 | 916 | 66 | 408 | 3.05e-58 | 209 |
| MS.gene064199.t1 | MTR_0931s0010 | 29.814 | 322 | 204 | 9 | 55 | 374 | 234 | 535 | 3.11e-29 | 126 |
| MS.gene064199.t1 | MTR_0931s0010 | 30.124 | 322 | 202 | 8 | 572 | 891 | 235 | 535 | 1.19e-26 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064199.t1 | AT4G28220 | 72.358 | 492 | 135 | 1 | 59 | 549 | 52 | 543 | 0.0 | 754 |
| MS.gene064199.t1 | AT4G28220 | 61.885 | 488 | 186 | 0 | 575 | 1062 | 52 | 539 | 0.0 | 652 |
| MS.gene064199.t1 | AT4G21490 | 67.115 | 520 | 164 | 3 | 549 | 1062 | 30 | 548 | 0.0 | 716 |
| MS.gene064199.t1 | AT4G21490 | 61.818 | 550 | 203 | 4 | 5 | 549 | 5 | 552 | 0.0 | 689 |
| MS.gene064199.t1 | AT4G05020 | 67.686 | 523 | 158 | 4 | 549 | 1062 | 30 | 550 | 0.0 | 715 |
| MS.gene064199.t1 | AT4G05020 | 61.121 | 553 | 204 | 6 | 5 | 549 | 5 | 554 | 0.0 | 681 |
| MS.gene064199.t1 | AT4G21490 | 67.115 | 520 | 163 | 4 | 549 | 1062 | 30 | 547 | 0.0 | 712 |
| MS.gene064199.t1 | AT4G21490 | 61.818 | 550 | 202 | 5 | 5 | 549 | 5 | 551 | 0.0 | 684 |
| MS.gene064199.t1 | AT2G20800 | 65.169 | 534 | 174 | 5 | 538 | 1062 | 20 | 550 | 0.0 | 707 |
| MS.gene064199.t1 | AT2G20800 | 58.115 | 573 | 227 | 7 | 1 | 565 | 1 | 568 | 0.0 | 654 |
| MS.gene064199.t1 | AT4G05020 | 63.214 | 560 | 158 | 5 | 549 | 1062 | 30 | 587 | 0.0 | 696 |
| MS.gene064199.t1 | AT4G05020 | 57.288 | 590 | 204 | 7 | 5 | 549 | 5 | 591 | 0.0 | 662 |
| MS.gene064199.t1 | AT4G28220 | 70.861 | 453 | 131 | 1 | 59 | 510 | 52 | 504 | 0.0 | 678 |
| MS.gene064199.t1 | AT4G28220 | 60.573 | 454 | 179 | 0 | 575 | 1028 | 52 | 505 | 0.0 | 589 |
| MS.gene064199.t1 | AT2G29990 | 41.056 | 341 | 183 | 8 | 37 | 372 | 55 | 382 | 1.86e-61 | 219 |
| MS.gene064199.t1 | AT2G29990 | 39.118 | 340 | 183 | 9 | 560 | 889 | 57 | 382 | 5.71e-56 | 203 |
| MS.gene064199.t1 | AT1G07180 | 40.173 | 346 | 185 | 9 | 34 | 372 | 54 | 384 | 9.02e-59 | 211 |
| MS.gene064199.t1 | AT1G07180 | 38.485 | 330 | 181 | 8 | 567 | 889 | 70 | 384 | 3.26e-54 | 198 |
| MS.gene064199.t1 | AT5G08740 | 24.485 | 437 | 267 | 19 | 507 | 908 | 12 | 420 | 1.33e-12 | 72.0 |
Find 241 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAAAAGGTATAAGCATTT+TGG | 0.113669 | 4.2:-56085816 | MS.gene064199:intron |
| CTGACCACTTCCTCTTTCTT+TGG | 0.161319 | 4.2:+56081492 | None:intergenic |
| GAAATGGAGAAATAAAATTT+TGG | 0.187748 | 4.2:-56088792 | MS.gene064199:CDS |
| AGCACTACCACTTTCTTTCT+TGG | 0.213188 | 4.2:+56083084 | None:intergenic |
| TTCTCTGGATTATGACTTCT+TGG | 0.237305 | 4.2:-56088683 | MS.gene064199:CDS |
| TTCATAAAATCGAGTATTTC+AGG | 0.240335 | 4.2:+56081415 | None:intergenic |
| AGATGGAATTGATGTGAAAT+TGG | 0.251162 | 4.2:-56081554 | MS.gene064199:CDS |
| CGGAAGGCCCTCTAAGGTTT+AGG | 0.253205 | 4.2:-56080230 | MS.gene064199:CDS |
| TCCATAAAATCTCTTATAAC+TGG | 0.256424 | 4.2:+56087607 | None:intergenic |
| ACAAGTGACACTCGGTAATA+AGG | 0.258096 | 4.2:+56089318 | None:intergenic |
| TGGGGAAGGCGATTCGTATT+TGG | 0.259196 | 4.2:-56079502 | MS.gene064199:CDS |
| GAAAATCGTCGAGGTCCTTC+TGG | 0.265610 | 4.2:+56086552 | None:intergenic |
| ATAATTTCTTCAAATCTTCT+TGG | 0.269593 | 4.2:+56088131 | None:intergenic |
| TGCTTTATGTGCAGACTAAA+AGG | 0.276588 | 4.2:-56087371 | MS.gene064199:intron |
| TTCTGCTTCTTTGTACTATC+AGG | 0.282569 | 4.2:-56090537 | MS.gene064199:intron |
| GGGTGATGATCTTCAATATT+AGG | 0.302508 | 4.2:+56089463 | None:intergenic |
| TGATATCATGGACATATTTA+AGG | 0.307481 | 4.2:-56086991 | MS.gene064199:CDS |
| GGCTGCTACCAGTTTCCTTA+AGG | 0.325292 | 4.2:-56089404 | MS.gene064199:CDS |
| GATCGATGCTGCAGAGAAAA+AGG | 0.327450 | 4.2:-56088752 | MS.gene064199:CDS |
| TATAAATCAGCGTAGAATTA+TGG | 0.331406 | 4.2:-56081190 | MS.gene064199:intron |
| GGTCCGAAACATCATTAAAA+AGG | 0.335197 | 4.2:-56089263 | MS.gene064199:intron |
| TTCGAATTCTTCTACAGTTA+AGG | 0.336831 | 4.2:+56086944 | None:intergenic |
| AGAAAAGAAATAGACATAGA+AGG | 0.337515 | 4.2:-56086813 | MS.gene064199:CDS |
| TGTTTATGTGATAGATTAAT+CGG | 0.340797 | 4.2:-56081287 | MS.gene064199:intron |
| TACCAGTTTCCTTAAGGGTT+TGG | 0.342817 | 4.2:-56089398 | MS.gene064199:CDS |
| CAGCTCAGCTTCCGGGAGAT+TGG | 0.344513 | 4.2:-56079719 | MS.gene064199:CDS |
| ACAAACAGCAGCTCAGCTTC+CGG | 0.350319 | 4.2:-56079727 | MS.gene064199:CDS |
| AAAGAATTTCAAAACGTTGT+TGG | 0.350665 | 4.2:-56080774 | MS.gene064199:CDS |
| AGCTCAGCTTCCGGGAGATT+GGG | 0.355497 | 4.2:-56079718 | MS.gene064199:CDS |
| AGCTTTGGTGGTATCCGATT+GGG | 0.357196 | 4.2:-56079521 | MS.gene064199:CDS |
| GGAGGCTTGCTCTTTCAAAA+AGG | 0.357921 | 4.2:+56082107 | None:intergenic |
| TGCCTCATGGGATGGTAGTT+TGG | 0.358988 | 4.2:-56081459 | MS.gene064199:CDS |
| GCTGCTACCAGTTTCCTTAA+GGG | 0.367418 | 4.2:-56089403 | MS.gene064199:CDS |
| ATTCATACCTGTGCAGTTGC+TGG | 0.368331 | 4.2:+56086744 | None:intergenic |
| CCGGGAGATTGGGTTTCAAT+TGG | 0.368827 | 4.2:-56079708 | MS.gene064199:CDS |
| GCCATCAGTTTCATGCCTTC+AGG | 0.369539 | 4.2:-56080197 | MS.gene064199:intron |
| TGCCCCATGGATTGATTATC+TGG | 0.373588 | 4.2:-56087651 | MS.gene064199:CDS |
| TTCTTTAAGATCTAACATTC+CGG | 0.374413 | 4.2:+56080791 | None:intergenic |
| GTCACAGCACCCAATGGCTT+TGG | 0.376829 | 4.2:-56085728 | MS.gene064199:CDS |
| TTTCAATACCCCTGGTGTTA+AGG | 0.376934 | 4.2:-56088632 | MS.gene064199:CDS |
| GGAAGGCCCTCTAAGGTTTA+GGG | 0.378458 | 4.2:-56080229 | MS.gene064199:CDS |
| TTTGGACAGTTTGCTCCTCT+CGG | 0.382469 | 4.2:-56079756 | MS.gene064199:CDS |
| TTGGACAGTTTGCTCCTCTC+GGG | 0.386811 | 4.2:-56079755 | MS.gene064199:CDS |
| CAGTCAGTGGTTGTGGTATT+CGG | 0.389525 | 4.2:-56079679 | MS.gene064199:intron |
| TCAATGTCCACTTCTGTAGT+AGG | 0.392680 | 4.2:+56080657 | None:intergenic |
| ATCCAAACCCTTAAGGAAAC+TGG | 0.396227 | 4.2:+56089396 | None:intergenic |
| TACGAATCGCCTTCCCCAAT+CGG | 0.399877 | 4.2:+56079507 | None:intergenic |
| AATATCCGGAAGGCCCTCTA+AGG | 0.400293 | 4.2:-56080236 | MS.gene064199:CDS |
| AAAGTCGTGGTCCTTGGAAC+TGG | 0.400385 | 4.2:-56089430 | MS.gene064199:CDS |
| CCTTTCTGTTGCAGATTCCC+AGG | 0.415308 | 4.2:-56086778 | MS.gene064199:CDS |
| CAAACAGCAGCTCAGCTTCC+GGG | 0.415661 | 4.2:-56079726 | MS.gene064199:CDS |
| GGGATGGTAGTTTGGTCAAC+TGG | 0.419171 | 4.2:-56081451 | MS.gene064199:CDS |
| GAAACATATACTTGATTTGA+GGG | 0.422160 | 4.2:-56086865 | MS.gene064199:CDS |
| ATCATCGGTTTCGTCCCTTC+CGG | 0.425142 | 4.2:-56086518 | MS.gene064199:intron |
| CAAGTGACACTCGGTAATAA+GGG | 0.426656 | 4.2:+56089319 | None:intergenic |
| ATTGAAACAGTCTGCAAGAT+AGG | 0.432669 | 4.2:+56080278 | None:intergenic |
| ATTGTTGGTGGTGGTCCAAC+TGG | 0.433243 | 4.2:-56082040 | MS.gene064199:CDS |
| GAAAAGAAATAGACATAGAA+GGG | 0.433481 | 4.2:-56086812 | MS.gene064199:CDS |
| AAGATAACACTAATTCAATC+TGG | 0.440272 | 4.2:-56088085 | MS.gene064199:CDS |
| CTAAACCTTAGAGGGCCTTC+CGG | 0.445137 | 4.2:+56080231 | None:intergenic |
| CCTGTTCCCCTAAACCTTAG+AGG | 0.445725 | 4.2:+56080222 | None:intergenic |
| AGCAGAAAGTTGGAAGAGAA+AGG | 0.447754 | 4.2:+56090553 | None:intergenic |
| GTGCTTGGAACTGGTTGGGC+TGG | 0.448822 | 4.2:-56083066 | MS.gene064199:CDS |
| TGATGTGAAATTGGGATCAA+TGG | 0.449893 | 4.2:-56081545 | MS.gene064199:CDS |
| CAGGTTGTTACTATGCCTCA+TGG | 0.451645 | 4.2:-56081472 | MS.gene064199:CDS |
| CCTTCTTCAACAAAGCCTCC+AGG | 0.456795 | 4.2:-56090591 | MS.gene064199:CDS |
| CAGCAGAACTACCTGGAGAC+TGG | 0.457833 | 4.2:-56085761 | MS.gene064199:CDS |
| CCTCTAAGGTTTAGGGGAAC+AGG | 0.458851 | 4.2:-56080222 | MS.gene064199:CDS |
| GATGGAATTGATGTGAAATT+GGG | 0.458874 | 4.2:-56081553 | MS.gene064199:CDS |
| GACACAGCAGTCAGTGGTTG+TGG | 0.459107 | 4.2:-56079686 | MS.gene064199:CDS |
| CTGGAGAACAAGAGCTTTGG+TGG | 0.459744 | 4.2:-56079533 | MS.gene064199:CDS |
| TGCAGACTGTTTCAATCGCA+TGG | 0.462033 | 4.2:-56080271 | MS.gene064199:CDS |
| TTTCATCTGAGAATCAACTT+TGG | 0.463383 | 4.2:+56080614 | None:intergenic |
| AGCACGGAGCATCGTAGAAC+CGG | 0.466245 | 4.2:-56089284 | MS.gene064199:CDS |
| GTCTAATATTGAAAACTTAG+TGG | 0.466929 | 4.2:-56088719 | MS.gene064199:CDS |
| GTAGTTTGGTCAACTGGTAT+TGG | 0.467998 | 4.2:-56081445 | MS.gene064199:CDS |
| ACAATGAAGGTGAAATCAAC+GGG | 0.470147 | 4.2:-56087688 | MS.gene064199:CDS |
| TACTAAGTAATCATAATCAA+TGG | 0.471810 | 4.2:+56082672 | None:intergenic |
| CCTGGAGGCTTTGTTGAAGA+AGG | 0.475102 | 4.2:+56090591 | None:intergenic |
| GGAACATGCCTACTTCTTGA+AGG | 0.476442 | 4.2:-56082602 | MS.gene064199:intron |
| ATGAACCATCCAAACCCTTA+AGG | 0.478727 | 4.2:+56089389 | None:intergenic |
| TTTAAATAAATGTCCACTTG+TGG | 0.478762 | 4.2:+56080732 | None:intergenic |
| AGGGTGAATGGTGAGGGCCC+TGG | 0.480488 | 4.2:+56090573 | None:intergenic |
| TTCTCTTCCACTGGTACAGA+AGG | 0.480719 | 4.2:+56082085 | None:intergenic |
| GGTAGTGCTTGGAACTGGTT+GGG | 0.480820 | 4.2:-56083070 | MS.gene064199:CDS |
| GAATGTACCTGAGCAGTTGC+TGG | 0.481172 | 4.2:+56080585 | None:intergenic |
| TATCTTGACTTTATCTTGAA+CGG | 0.481241 | 4.2:+56088102 | None:intergenic |
| GATGAATGGTTGAGAGTAAA+GGG | 0.482352 | 4.2:-56087333 | MS.gene064199:CDS |
| AGATGGTATAGAAGTTCAAA+CGG | 0.484698 | 4.2:-56087746 | MS.gene064199:CDS |
| TTGCTGGAAGAGTCTTCACC+TGG | 0.485095 | 4.2:+56086760 | None:intergenic |
| TGATGAATGGTTGAGAGTAA+AGG | 0.485744 | 4.2:-56087334 | MS.gene064199:CDS |
| AGTACAAAGAAGCAGAAAGT+TGG | 0.485887 | 4.2:+56090543 | None:intergenic |
| TTGACTTTATCTTGAACGGT+TGG | 0.486007 | 4.2:+56088106 | None:intergenic |
| TATTACCGAGTGTCACTTGT+GGG | 0.486394 | 4.2:-56089315 | MS.gene064199:CDS |
| GGATCAATGGTTGTGAAAGT+TGG | 0.491130 | 4.2:-56081532 | MS.gene064199:CDS |
| CAGCTGGAGAACAAGAGCTT+TGG | 0.491737 | 4.2:-56079536 | MS.gene064199:CDS |
| AGTTTCCTTAAGGGTTTGGA+TGG | 0.492808 | 4.2:-56089394 | MS.gene064199:CDS |
| ATTGCTTACCTTCAAGAAGT+AGG | 0.493966 | 4.2:+56082594 | None:intergenic |
| GATATCTCATCCAAAGAAAG+AGG | 0.496111 | 4.2:-56081502 | MS.gene064199:CDS |
| CAAGCAGCAGCAGAACTACC+TGG | 0.498700 | 4.2:-56085768 | MS.gene064199:CDS |
| GCGGTTTGCTCGGTGCCCCA+TGG | 0.498753 | 4.2:-56087664 | MS.gene064199:CDS |
| GTGTGACAACATCTATGCTC+TGG | 0.499109 | 4.2:-56081226 | MS.gene064199:CDS |
| TTTAGATAGTATTCAACTTG+AGG | 0.503159 | 4.2:+56086891 | None:intergenic |
| GAATTGCAGTGGTGGAGGTG+TGG | 0.503907 | 4.2:-56089521 | MS.gene064199:intron |
| GTTGTTGGAGGAGGTCCAAC+TGG | 0.504579 | 4.2:-56088187 | MS.gene064199:CDS |
| TTGGATAAAATCATGCAGTT+CGG | 0.505426 | 4.2:+56088150 | None:intergenic |
| GGAATTGTGTGAGAAATATC+CGG | 0.505970 | 4.2:-56080250 | MS.gene064199:CDS |
| GAGCTTTGGTGGTATCCGAT+TGG | 0.506991 | 4.2:-56079522 | MS.gene064199:CDS |
| ATCAACGGGAGCGGTTTGCT+CGG | 0.508720 | 4.2:-56087674 | MS.gene064199:CDS |
| CAATGTCCACTTCTGTAGTA+GGG | 0.509746 | 4.2:+56080658 | None:intergenic |
| GCAGAAAGTTGGAAGAGAAA+GGG | 0.510279 | 4.2:+56090554 | None:intergenic |
| TTTGGGCAATTTGCTCCACT+GGG | 0.512737 | 4.2:-56085798 | MS.gene064199:CDS |
| TGATTACTTAGTAATAGCTA+TGG | 0.512988 | 4.2:-56082662 | MS.gene064199:CDS |
| GAACAAAAGTTCAAAAGAGA+TGG | 0.513759 | 4.2:-56081571 | MS.gene064199:CDS |
| AGAAACATATACTTGATTTG+AGG | 0.514547 | 4.2:-56086866 | MS.gene064199:CDS |
| AATAAATCAACGTAAAATCA+TGG | 0.516150 | 4.2:-56087274 | MS.gene064199:intron |
| TCGTGGTCCTTGGAACTGGA+TGG | 0.516860 | 4.2:-56089426 | MS.gene064199:CDS |
| CCTGGAGACTGGGTTTCCAT+TGG | 0.517640 | 4.2:-56085750 | MS.gene064199:CDS |
| TGGTGGTATCCGATTGGGGA+AGG | 0.520931 | 4.2:-56079516 | MS.gene064199:CDS |
| GGTGGAAGTTTAGCTGCATA+TGG | 0.521005 | 4.2:-56083147 | MS.gene064199:CDS |
| ATTGAAAACTTAGTGGGAAG+TGG | 0.521615 | 4.2:-56088712 | MS.gene064199:CDS |
| CTTGGGAATTGCAGTGGTGG+AGG | 0.522174 | 4.2:-56089526 | MS.gene064199:intron |
| TCTAATATTGAAAACTTAGT+GGG | 0.522285 | 4.2:-56088718 | MS.gene064199:CDS |
| CATGCAGCAAACTAGTCAGC+TGG | 0.522547 | 4.2:-56079552 | MS.gene064199:intron |
| TTGCAGTGGTGGAGGTGTGG+TGG | 0.523840 | 4.2:-56089518 | MS.gene064199:intron |
| AAGATTACTCTCCTCGAGGC+TGG | 0.525300 | 4.2:-56081938 | MS.gene064199:CDS |
| AGAAAGAAAGTGGTAGTGCT+TGG | 0.526927 | 4.2:-56083081 | MS.gene064199:CDS |
| TACAGAATACCAAAGCCATT+GGG | 0.527108 | 4.2:+56085719 | None:intergenic |
| CTGCTTCTAGGATGATATCA+TGG | 0.528268 | 4.2:-56087003 | MS.gene064199:intron |
| GGATTGATTATCTGGTCCAC+TGG | 0.529835 | 4.2:-56087643 | MS.gene064199:CDS |
| AAGGCAGACAAGGACAGTTC+CGG | 0.530156 | 4.2:-56080810 | MS.gene064199:CDS |
| TCATCCAAAGAAAGAGGAAG+TGG | 0.530620 | 4.2:-56081496 | MS.gene064199:CDS |
| CTGTTCCCCTAAACCTTAGA+GGG | 0.533525 | 4.2:+56080223 | None:intergenic |
| GTTGGAAGAGAAAGGGTGAA+TGG | 0.534449 | 4.2:+56090561 | None:intergenic |
| ATTTGGGTGAATATGACGAT+TGG | 0.536274 | 4.2:-56090616 | None:intergenic |
| GAGAAGAAGTTTACAAGAGA+TGG | 0.536920 | 4.2:-56087763 | MS.gene064199:CDS |
| CAAAGAAAGAGGAAGTGGTC+AGG | 0.537184 | 4.2:-56081491 | MS.gene064199:CDS |
| CCAATGGAAACCCAGTCTCC+AGG | 0.538744 | 4.2:+56085750 | None:intergenic |
| ATACAGAATACCAAAGCCAT+TGG | 0.539544 | 4.2:+56085718 | None:intergenic |
| TACTTGATTTGAGGGTTCTG+TGG | 0.539810 | 4.2:-56086857 | MS.gene064199:CDS |
| TGACAAGGAAATTACAATGA+AGG | 0.542053 | 4.2:-56087701 | MS.gene064199:CDS |
| CTGGGAATCTGCAACAGAAA+GGG | 0.543531 | 4.2:+56086779 | None:intergenic |
| TGCTCCTCTTCAGATTGACT+CGG | 0.543713 | 4.2:+56088232 | None:intergenic |
| AGCAGAACTACCTGGAGACT+GGG | 0.544027 | 4.2:-56085760 | MS.gene064199:CDS |
| AAATCTACCAGCAACTGCTC+AGG | 0.544227 | 4.2:-56080592 | MS.gene064199:intron |
| CCTGGGAATCTGCAACAGAA+AGG | 0.545647 | 4.2:+56086778 | None:intergenic |
| TTGAGATTTACTTAGCAAAG+TGG | 0.547247 | 4.2:+56080686 | None:intergenic |
| CTTCTTCAACAAAGCCTCCA+GGG | 0.547343 | 4.2:-56090590 | MS.gene064199:CDS |
| GCAGACTAAAAGGCATGTAC+TGG | 0.547750 | 4.2:-56087361 | MS.gene064199:intron |
| GCTGCTTGCTCTCCACCCAG+TGG | 0.548772 | 4.2:+56085783 | None:intergenic |
| TGGACCAGATAATCAATCCA+TGG | 0.551908 | 4.2:+56087647 | None:intergenic |
| CCAATTGAAACCCAATCTCC+CGG | 0.553076 | 4.2:+56079708 | None:intergenic |
| CGTGGTCCTTGGAACTGGAT+GGG | 0.554266 | 4.2:-56089425 | MS.gene064199:CDS |
| TTTCAGGATCTGGACATCAT+CGG | 0.554643 | 4.2:-56086533 | MS.gene064199:CDS |
| GACTTCTTGGTTGTAGCAGT+AGG | 0.555062 | 4.2:-56088670 | MS.gene064199:CDS |
| GATGAATGGCTAAGGGTGGA+AGG | 0.556348 | 4.2:-56081249 | MS.gene064199:CDS |
| TTCCACCCTTAGCCATTCAT+CGG | 0.557506 | 4.2:+56081251 | None:intergenic |
| TCAAAGACCAAGAAAGAAAG+TGG | 0.557967 | 4.2:-56083091 | MS.gene064199:CDS |
| GATGGTATAGAAGTTCAAAC+GGG | 0.560775 | 4.2:-56087745 | MS.gene064199:CDS |
| TTATTACCGAGTGTCACTTG+TGG | 0.560816 | 4.2:-56089316 | MS.gene064199:CDS |
| CCATTGGTCACAGCACCCAA+TGG | 0.560994 | 4.2:-56085734 | MS.gene064199:CDS |
| TGTGAAGATGTGTTTGCCAT+TGG | 0.561141 | 4.2:-56087309 | MS.gene064199:CDS |
| TGGTAGTGCTTGGAACTGGT+TGG | 0.561852 | 4.2:-56083071 | MS.gene064199:CDS |
| GACTCTTCCAGCAACTGCAC+AGG | 0.562506 | 4.2:-56086751 | MS.gene064199:intron |
| TGTTACTATGCCTCATGGGA+TGG | 0.563772 | 4.2:-56081467 | MS.gene064199:CDS |
| GTAAATACTTTCAATACCCC+TGG | 0.564589 | 4.2:-56088640 | MS.gene064199:CDS |
| AGAAGAATTCGAAGAAGTGA+TGG | 0.565316 | 4.2:-56086934 | MS.gene064199:CDS |
| AGGTTGTTACTATGCCTCAT+GGG | 0.565524 | 4.2:-56081471 | MS.gene064199:CDS |
| GGACCAGATAATCAATCCAT+GGG | 0.568461 | 4.2:+56087648 | None:intergenic |
| AGACATAGAAGGGTTTAAGC+TGG | 0.568776 | 4.2:-56086802 | MS.gene064199:CDS |
| GCTGCAAATTCAACACCAGT+TGG | 0.569812 | 4.2:+56088172 | None:intergenic |
| TTTGGTATTCTGTATATGCA+AGG | 0.572972 | 4.2:-56085710 | MS.gene064199:intron |
| AGAATGCCCTACTACAGAAG+TGG | 0.574073 | 4.2:-56080664 | MS.gene064199:CDS |
| TACAATGAAGGTGAAATCAA+CGG | 0.574139 | 4.2:-56087689 | MS.gene064199:CDS |
| GCAGCAAACTCTACTCCAGT+TGG | 0.575986 | 4.2:+56082025 | None:intergenic |
| CCATTGGGTGCTGTGACCAA+TGG | 0.577682 | 4.2:+56085734 | None:intergenic |
| GATTACTTAGTAATAGCTAT+GGG | 0.579165 | 4.2:-56082661 | MS.gene064199:CDS |
| GTGCATTGGTGACCGATGAA+TGG | 0.583214 | 4.2:-56081263 | MS.gene064199:CDS |
| TGGTGACCGATGAATGGCTA+AGG | 0.583842 | 4.2:-56081257 | MS.gene064199:CDS |
| CAATTGGACACAGCAGTCAG+TGG | 0.584037 | 4.2:-56079692 | MS.gene064199:CDS |
| AAAGTGGTAGTGCTTGGAAC+TGG | 0.584146 | 4.2:-56083075 | MS.gene064199:CDS |
| GCTTTGGTGGTATCCGATTG+GGG | 0.584873 | 4.2:-56079520 | MS.gene064199:CDS |
| TATTGATGAACAATCACCAA+TGG | 0.585097 | 4.2:+56087293 | None:intergenic |
| CAAGTAACACTTGGTAACAA+AGG | 0.589434 | 4.2:+56082964 | None:intergenic |
| AGAGAAAGGGTGAATGGTGA+GGG | 0.590542 | 4.2:+56090567 | None:intergenic |
| GAAGGCCCTCTAAGGTTTAG+GGG | 0.591118 | 4.2:-56080228 | MS.gene064199:CDS |
| GGGCAATTTGCTCCACTGGG+TGG | 0.593754 | 4.2:-56085795 | MS.gene064199:CDS |
| CGAGGAGAGTAATCTTGACA+TGG | 0.595304 | 4.2:+56081945 | None:intergenic |
| GTGAATGGTGAGGGCCCTGG+AGG | 0.595344 | 4.2:+56090576 | None:intergenic |
| GTCAATCTGAAGAGGAGCAA+AGG | 0.596000 | 4.2:-56088228 | MS.gene064199:CDS |
| TTGACATGGTCTATAAGAGA+AGG | 0.596187 | 4.2:+56081959 | None:intergenic |
| ATAGATTAATCGGCGTGCAT+TGG | 0.598331 | 4.2:-56081277 | MS.gene064199:intron |
| ATGTACTGGCAACTGATGAA+TGG | 0.599584 | 4.2:-56087347 | MS.gene064199:CDS |
| CGCGATTAAAGCAACTTGCT+AGG | 0.599701 | 4.2:+56086595 | None:intergenic |
| TGGACAGTTTGCTCCTCTCG+GGG | 0.600681 | 4.2:-56079754 | MS.gene064199:CDS |
| AAGATAGGGGTTTACCGGAA+GGG | 0.601174 | 4.2:+56086504 | None:intergenic |
| TGTAATAGATTGTCTTGAGA+AGG | 0.601188 | 4.2:-56088263 | MS.gene064199:CDS |
| TGACAGTTCTCCTTAACACC+AGG | 0.601556 | 4.2:+56088622 | None:intergenic |
| GAAGGTGAAATCAACGGGAG+CGG | 0.608655 | 4.2:-56087683 | MS.gene064199:CDS |
| TTGTTACCAAGTGTTACTTG+TGG | 0.614398 | 4.2:-56082961 | MS.gene064199:CDS |
| TTCTAGGTAGCTGCTCAACA+AGG | 0.618079 | 4.2:-56080303 | MS.gene064199:intron |
| TGTGACAACATCTATGCTCT+GGG | 0.620185 | 4.2:-56081225 | MS.gene064199:CDS |
| AAGAGAAAGGGTGAATGGTG+AGG | 0.623100 | 4.2:+56090566 | None:intergenic |
| CTTCCACTGGTACAGAAGGG+AGG | 0.623592 | 4.2:+56082089 | None:intergenic |
| TCTTCCGAGTCAATCTGAAG+AGG | 0.623685 | 4.2:-56088236 | MS.gene064199:CDS |
| ATATAGGTTGCTGCGCAACA+AGG | 0.625684 | 4.2:-56086624 | MS.gene064199:intron |
| ATGAATGGCTAAGGGTGGAA+GGG | 0.629064 | 4.2:-56081248 | MS.gene064199:CDS |
| GCTGTTTGTTCTGCCCCGAG+AGG | 0.629289 | 4.2:+56079741 | None:intergenic |
| TCTCTTCCACTGGTACAGAA+GGG | 0.629584 | 4.2:+56082086 | None:intergenic |
| TAGCAGCCCATCCAGTTCCA+AGG | 0.629847 | 4.2:+56089419 | None:intergenic |
| CTTGTGGGTCAGTTGAAGCA+CGG | 0.632171 | 4.2:-56089300 | MS.gene064199:CDS |
| AAGCCTCCCTTCTGTACCAG+TGG | 0.633348 | 4.2:-56082092 | MS.gene064199:CDS |
| TGTTACTTGTGGCACTGTCG+AGG | 0.634203 | 4.2:-56082950 | MS.gene064199:CDS |
| TGCTGGAAGAGTCTTCACCT+GGG | 0.634925 | 4.2:+56086761 | None:intergenic |
| TAGGCATGTTCCTCAACACC+AGG | 0.635257 | 4.2:+56082613 | None:intergenic |
| GATATTTAACAAGGCAGACA+AGG | 0.635838 | 4.2:-56080820 | MS.gene064199:CDS |
| TATTGAGAGGTATCCACAAG+TGG | 0.637539 | 4.2:-56080745 | MS.gene064199:CDS |
| ACCTGAAGGCATGAAACTGA+TGG | 0.638521 | 4.2:+56080196 | None:intergenic |
| GATACTGAAGAGCATCCAGA+AGG | 0.642109 | 4.2:-56086567 | MS.gene064199:CDS |
| TATAGCTGTGATATTTAACA+AGG | 0.657322 | 4.2:-56080829 | MS.gene064199:CDS |
| GACAGTTCTCCTTAACACCA+GGG | 0.658869 | 4.2:+56088623 | None:intergenic |
| TTGTTGGCGACATTATTGAG+AGG | 0.659255 | 4.2:-56080758 | MS.gene064199:CDS |
| GAAGAATCAAATGAAAGACA+TGG | 0.659440 | 4.2:-56080709 | MS.gene064199:CDS |
| ACAGTGCCACAAGTAACACT+TGG | 0.664843 | 4.2:+56082955 | None:intergenic |
| GACCGATGAATGGCTAAGGG+TGG | 0.672269 | 4.2:-56081253 | MS.gene064199:CDS |
| ACTGACCCACAAGTGACACT+CGG | 0.681921 | 4.2:+56089310 | None:intergenic |
| TGTCATCAACACTCATAACA+CGG | 0.684459 | 4.2:+56087719 | None:intergenic |
| AGATATGATCACCAGCCTCG+AGG | 0.684689 | 4.2:+56081927 | None:intergenic |
| TAATCACTTATAGAATGTCG+AGG | 0.687947 | 4.2:-56088308 | MS.gene064199:intron |
| TTGTGTGAGAAATATCCGGA+AGG | 0.690898 | 4.2:-56080246 | MS.gene064199:CDS |
| TGTTATGAGTGTTGATGACA+AGG | 0.693444 | 4.2:-56087716 | MS.gene064199:CDS |
| GGTGACCGATGAATGGCTAA+GGG | 0.698885 | 4.2:-56081256 | MS.gene064199:CDS |
| TGGAAGAGTAGAAATGCCAG+TGG | 0.705182 | 4.2:+56087627 | None:intergenic |
| ACAGTTCTCCTTAACACCAG+GGG | 0.714844 | 4.2:+56088624 | None:intergenic |
| TGTCAAGATTACTCTCCTCG+AGG | 0.721601 | 4.2:-56081942 | MS.gene064199:CDS |
| GACCAAACTACCATCCCATG+AGG | 0.728671 | 4.2:+56081457 | None:intergenic |
| GTAAATGCAAAGTAGTTACG+AGG | 0.734579 | 4.2:+56089343 | None:intergenic |
| GTTCTGTGGAACGACCCACA+AGG | 0.743834 | 4.2:-56086843 | MS.gene064199:CDS |
| CCAGATCCTGAAAATCGTCG+AGG | 0.744477 | 4.2:+56086543 | None:intergenic |
| GTGAATGCAAAATAGTTACG+TGG | 0.750279 | 4.2:+56082988 | None:intergenic |
| GACCAGATAATCAATCCATG+GGG | 0.756544 | 4.2:+56087649 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 56079433 | 56090626 | 56079433 | ID=MS.gene064199 |
| chr4.2 | mRNA | 56079433 | 56090626 | 56079433 | ID=MS.gene064199.t1;Parent=MS.gene064199 |
| chr4.2 | exon | 56090538 | 56090626 | 56090538 | ID=MS.gene064199.t1.exon1;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56090538 | 56090626 | 56090538 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56089264 | 56089534 | 56089264 | ID=MS.gene064199.t1.exon2;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56089264 | 56089534 | 56089264 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56088612 | 56088815 | 56088612 | ID=MS.gene064199.t1.exon3;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56088612 | 56088815 | 56088612 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56088067 | 56088317 | 56088067 | ID=MS.gene064199.t1.exon4;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56088067 | 56088317 | 56088067 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56087594 | 56087813 | 56087594 | ID=MS.gene064199.t1.exon5;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56087594 | 56087813 | 56087594 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56087275 | 56087379 | 56087275 | ID=MS.gene064199.t1.exon6;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56087275 | 56087379 | 56087275 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56086752 | 56087015 | 56086752 | ID=MS.gene064199.t1.exon7;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56086752 | 56087015 | 56086752 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56086519 | 56086640 | 56086519 | ID=MS.gene064199.t1.exon8;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56086519 | 56086640 | 56086519 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56085711 | 56085830 | 56085711 | ID=MS.gene064199.t1.exon9;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56085711 | 56085830 | 56085711 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56082909 | 56083170 | 56082909 | ID=MS.gene064199.t1.exon10;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56082909 | 56083170 | 56082909 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56082603 | 56082809 | 56082603 | ID=MS.gene064199.t1.exon11;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56082603 | 56082809 | 56082603 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56081920 | 56082170 | 56081920 | ID=MS.gene064199.t1.exon12;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56081920 | 56082170 | 56081920 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56081402 | 56081621 | 56081402 | ID=MS.gene064199.t1.exon13;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56081402 | 56081621 | 56081402 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56081191 | 56081295 | 56081191 | ID=MS.gene064199.t1.exon14;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56081191 | 56081295 | 56081191 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56080593 | 56080856 | 56080593 | ID=MS.gene064199.t1.exon15;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56080593 | 56080856 | 56080593 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56080198 | 56080319 | 56080198 | ID=MS.gene064199.t1.exon16;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56080198 | 56080319 | 56080198 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56079680 | 56079788 | 56079680 | ID=MS.gene064199.t1.exon17;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56079680 | 56079788 | 56079680 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| chr4.2 | exon | 56079433 | 56079567 | 56079433 | ID=MS.gene064199.t1.exon18;Parent=MS.gene064199.t1 |
| chr4.2 | CDS | 56079433 | 56079567 | 56079433 | ID=cds.MS.gene064199.t1;Parent=MS.gene064199.t1 |
| Gene Sequence |
| Protein sequence |
