Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064563.t1 | KEH19492.1 | 83.5 | 158 | 7 | 3 | 44 | 182 | 43 | 200 | 6.30E-66 | 260.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064563.t1 | A0A072TQ21 | 83.5 | 158 | 7 | 3 | 44 | 182 | 43 | 200 | 4.6e-66 | 260.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene064563 | 0.808423 | 3.12E-50 | -1.69E-46 |
| MS.gene052954 | MS.gene064563 | 0.804258 | 2.36E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene064563 | 0.808311 | 3.30E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene064563 | 0.815654 | 8.28E-52 | -1.69E-46 |
| MS.gene055623 | MS.gene064563 | 0.800812 | 1.21E-48 | -1.69E-46 |
| MS.gene055841 | MS.gene064563 | 0.813616 | 2.34E-51 | -1.69E-46 |
| MS.gene057079 | MS.gene064563 | 0.802567 | 5.28E-49 | -1.69E-46 |
| MS.gene05795 | MS.gene064563 | 0.824588 | 7.44E-54 | -1.69E-46 |
| MS.gene059830 | MS.gene064563 | 0.819373 | 1.20E-52 | -1.69E-46 |
| MS.gene060297 | MS.gene064563 | 0.829387 | 5.30E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064563.t1 | MTR_8g461300 | 85.938 | 192 | 8 | 3 | 10 | 182 | 9 | 200 | 4.39e-115 | 325 |
| MS.gene064563.t1 | MTR_4g055800 | 52.096 | 167 | 66 | 6 | 24 | 180 | 130 | 292 | 1.48e-42 | 144 |
| MS.gene064563.t1 | MTR_3g080680 | 39.024 | 164 | 73 | 8 | 36 | 180 | 43 | 198 | 1.49e-19 | 82.0 |
| MS.gene064563.t1 | MTR_3g082420 | 33.175 | 211 | 107 | 8 | 1 | 180 | 14 | 221 | 1.93e-13 | 66.2 |
| MS.gene064563.t1 | MTR_3g083050 | 32.335 | 167 | 84 | 8 | 32 | 180 | 51 | 206 | 4.66e-12 | 62.4 |
| MS.gene064563.t1 | MTR_3g083080 | 34.483 | 174 | 83 | 10 | 24 | 180 | 46 | 205 | 3.55e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTTAAAAGTAATGTAATA+TGG | 0.262097 | 3.1:+41208370 | None:intergenic |
| TGGTTCTTTGTATACGAATA+AGG | 0.284069 | 3.1:-41208847 | MS.gene064563:CDS |
| GAATCTTCTGGATCCGGTTT+CGG | 0.312704 | 3.1:+41208948 | None:intergenic |
| TCACGACGCTTATACCAAAA+TGG | 0.333746 | 3.1:+41208870 | None:intergenic |
| TCACTATACTCAGAATCTTC+TGG | 0.339930 | 3.1:+41208936 | None:intergenic |
| ATGAAACACTCTTTGACAAC+AGG | 0.409120 | 3.1:+41208271 | None:intergenic |
| GCTGAAATCGGAACCGAAAC+CGG | 0.414648 | 3.1:-41208961 | MS.gene064563:CDS |
| CTACTTCTTTATATGCTCCT+AGG | 0.435411 | 3.1:-41208393 | MS.gene064563:CDS |
| TACTCAGAATCTTCTGGATC+CGG | 0.439841 | 3.1:+41208942 | None:intergenic |
| TGCAGAGGGGTTTGAGAGTA+CGG | 0.440533 | 3.1:+41208596 | None:intergenic |
| GGAGGAGAAACGGCTGAAAT+CGG | 0.450703 | 3.1:-41208973 | MS.gene064563:CDS |
| TTCATCTGTGGAGGAGGAGG+AGG | 0.455235 | 3.1:-41208991 | MS.gene064563:CDS |
| CACTGTCCACCACGTTGTGC+TGG | 0.467693 | 3.1:-41208655 | MS.gene064563:CDS |
| TACGCTGCAATACATGAGTC+TGG | 0.467899 | 3.1:+41208305 | None:intergenic |
| AAAGTAATGTAATATGGCCT+AGG | 0.487724 | 3.1:+41208376 | None:intergenic |
| CAATCAAACAAATATTAAGC+AGG | 0.490074 | 3.1:-41208554 | MS.gene064563:intron |
| TAAAGCAAAAGCAGAAGCAA+AGG | 0.500294 | 3.1:-41208353 | MS.gene064563:CDS |
| AGATTCATCTGTGGAGGAGG+AGG | 0.522683 | 3.1:-41208994 | MS.gene064563:CDS |
| GTCTGGAAAGTTGTTAGAGC+AGG | 0.523457 | 3.1:+41208322 | None:intergenic |
| GTTGTCAAGTGTACCCATCC+TGG | 0.532074 | 3.1:-41208418 | MS.gene064563:intron |
| AAAGCAAAAGCAGAAGCAAA+GGG | 0.538089 | 3.1:-41208352 | MS.gene064563:CDS |
| CTTTGTATACGAATAAGGCT+TGG | 0.542721 | 3.1:-41208842 | MS.gene064563:CDS |
| ACAAGATTCATCTGTGGAGG+AGG | 0.546974 | 3.1:-41208997 | MS.gene064563:CDS |
| CACATCATCGCTTAAGTCGA+GGG | 0.551344 | 3.1:+41208621 | None:intergenic |
| CTTCAAAGCAGCATTGCAGA+GGG | 0.559702 | 3.1:+41208582 | None:intergenic |
| GTATACGAATAAGGCTTGGA+AGG | 0.564676 | 3.1:-41208838 | MS.gene064563:CDS |
| GCATATAAAGAAGTAGCACC+AGG | 0.567371 | 3.1:+41208400 | None:intergenic |
| TGTCCACCACGTTGTGCTGG+CGG | 0.572730 | 3.1:-41208652 | MS.gene064563:CDS |
| TAAGGCTTGGAAGGATCGTA+AGG | 0.585446 | 3.1:-41208829 | MS.gene064563:CDS |
| TAAAGAAGTAGCACCAGGAT+GGG | 0.590468 | 3.1:+41208405 | None:intergenic |
| GCACATCATCGCTTAAGTCG+AGG | 0.599300 | 3.1:+41208620 | None:intergenic |
| ATAAAGAAGTAGCACCAGGA+TGG | 0.610411 | 3.1:+41208404 | None:intergenic |
| CAGCACAACGTGGTGGACAG+TGG | 0.610706 | 3.1:+41208656 | None:intergenic |
| GAAACAAGATTCATCTGTGG+AGG | 0.613961 | 3.1:-41209000 | MS.gene064563:CDS |
| TCTTCAAAGCAGCATTGCAG+AGG | 0.630589 | 3.1:+41208581 | None:intergenic |
| AGAAGATTCTGAGTATAGTG+AGG | 0.652619 | 3.1:-41208934 | MS.gene064563:CDS |
| GCAAAAGCAGAAGCAAAGGG+AGG | 0.667232 | 3.1:-41208349 | MS.gene064563:CDS |
| TGAGTATAGTGAGGAAGACG+AGG | 0.671624 | 3.1:-41208925 | MS.gene064563:CDS |
| CATGAAACAAGATTCATCTG+TGG | 0.677002 | 3.1:-41209003 | MS.gene064563:CDS |
| ATACCGCCAGCACAACGTGG+TGG | 0.689012 | 3.1:+41208649 | None:intergenic |
| ACATCATCGCTTAAGTCGAG+GGG | 0.690101 | 3.1:+41208622 | None:intergenic |
| TTCAAAGCAGCATTGCAGAG+GGG | 0.703047 | 3.1:+41208583 | None:intergenic |
| ACAATACCGCCAGCACAACG+TGG | 0.758364 | 3.1:+41208646 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GCTTTAAAAGTAATGTAATA+TGG | + | chr3.1:41208893-41208912 | None:intergenic | 20.0% |
| ! | ACTTTATCAAACACAAAAGT+TGG | + | chr3.1:41208824-41208843 | None:intergenic | 25.0% |
| ! | CAATCAAACAAATATTAAGC+AGG | - | chr3.1:41208706-41208725 | MS.gene064563:intron | 25.0% |
| !!! | CACTTAATTTTGATTGATTC+TGG | - | chr3.1:41208548-41208567 | MS.gene064563:intron | 25.0% |
| AAAGTAATGTAATATGGCCT+AGG | + | chr3.1:41208887-41208906 | None:intergenic | 30.0% | |
| CAAACAAATATTAAGCAGGT+TGG | - | chr3.1:41208710-41208729 | MS.gene064563:intron | 30.0% | |
| TGGTTCTTTGTATACGAATA+AGG | - | chr3.1:41208413-41208432 | MS.gene064563:intron | 30.0% | |
| ! | ACAATGACAGTTTTCCATTT+TGG | - | chr3.1:41208376-41208395 | MS.gene064563:CDS | 30.0% |
| AAAGCAAAAGCAGAAGCAAA+GGG | - | chr3.1:41208908-41208927 | MS.gene064563:CDS | 35.0% | |
| AGAAGATTCTGAGTATAGTG+AGG | - | chr3.1:41208326-41208345 | MS.gene064563:CDS | 35.0% | |
| AGCAAACTAAGTGTAAGTGT+AGG | - | chr3.1:41208483-41208502 | MS.gene064563:intron | 35.0% | |
| ATGAAACACTCTTTGACAAC+AGG | + | chr3.1:41208992-41209011 | None:intergenic | 35.0% | |
| CTACTTCTTTATATGCTCCT+AGG | - | chr3.1:41208867-41208886 | MS.gene064563:CDS | 35.0% | |
| CTTTGTATACGAATAAGGCT+TGG | - | chr3.1:41208418-41208437 | MS.gene064563:intron | 35.0% | |
| TAAAGCAAAAGCAGAAGCAA+AGG | - | chr3.1:41208907-41208926 | MS.gene064563:CDS | 35.0% | |
| TCACTATACTCAGAATCTTC+TGG | + | chr3.1:41208327-41208346 | None:intergenic | 35.0% | |
| TGGACAATACAATACAAAGC+AGG | - | chr3.1:41208568-41208587 | MS.gene064563:CDS | 35.0% | |
| ATAAAGAAGTAGCACCAGGA+TGG | + | chr3.1:41208859-41208878 | None:intergenic | 40.0% | |
| GAAACAAGATTCATCTGTGG+AGG | - | chr3.1:41208260-41208279 | MS.gene064563:CDS | 40.0% | |
| GCATATAAAGAAGTAGCACC+AGG | + | chr3.1:41208863-41208882 | None:intergenic | 40.0% | |
| GTATACGAATAAGGCTTGGA+AGG | - | chr3.1:41208422-41208441 | MS.gene064563:intron | 40.0% | |
| TAAAGAAGTAGCACCAGGAT+GGG | + | chr3.1:41208858-41208877 | None:intergenic | 40.0% | |
| TACTCAGAATCTTCTGGATC+CGG | + | chr3.1:41208321-41208340 | None:intergenic | 40.0% | |
| TCACGACGCTTATACCAAAA+TGG | + | chr3.1:41208393-41208412 | None:intergenic | 40.0% | |
| !! | TTTTGGTATAAGCGTCGTGA+TGG | - | chr3.1:41208393-41208412 | MS.gene064563:CDS | 40.0% |
| ACAAGATTCATCTGTGGAGG+AGG | - | chr3.1:41208263-41208282 | MS.gene064563:CDS | 45.0% | |
| ACATCATCGCTTAAGTCGAG+GGG | + | chr3.1:41208641-41208660 | None:intergenic | 45.0% | |
| CACATCATCGCTTAAGTCGA+GGG | + | chr3.1:41208642-41208661 | None:intergenic | 45.0% | |
| CTTCAAAGCAGCATTGCAGA+GGG | + | chr3.1:41208681-41208700 | None:intergenic | 45.0% | |
| GAATCTTCTGGATCCGGTTT+CGG | + | chr3.1:41208315-41208334 | None:intergenic | 45.0% | |
| GTCTGGAAAGTTGTTAGAGC+AGG | + | chr3.1:41208941-41208960 | None:intergenic | 45.0% | |
| TAAGGCTTGGAAGGATCGTA+AGG | - | chr3.1:41208431-41208450 | MS.gene064563:intron | 45.0% | |
| TACGCTGCAATACATGAGTC+TGG | + | chr3.1:41208958-41208977 | None:intergenic | 45.0% | |
| TCTTCAAAGCAGCATTGCAG+AGG | + | chr3.1:41208682-41208701 | None:intergenic | 45.0% | |
| TGAGTATAGTGAGGAAGACG+AGG | - | chr3.1:41208335-41208354 | MS.gene064563:CDS | 45.0% | |
| TTCAAAGCAGCATTGCAGAG+GGG | + | chr3.1:41208680-41208699 | None:intergenic | 45.0% | |
| !! | TACTTATTTATTATTAATTA+GGG | - | chr3.1:41208797-41208816 | MS.gene064563:CDS | 5.0% |
| !! | TTACTTATTTATTATTAATT+AGG | - | chr3.1:41208796-41208815 | MS.gene064563:CDS | 5.0% |
| AGATTCATCTGTGGAGGAGG+AGG | - | chr3.1:41208266-41208285 | MS.gene064563:CDS | 50.0% | |
| GCAAAAGCAGAAGCAAAGGG+AGG | - | chr3.1:41208911-41208930 | MS.gene064563:CDS | 50.0% | |
| GCACATCATCGCTTAAGTCG+AGG | + | chr3.1:41208643-41208662 | None:intergenic | 50.0% | |
| GCTGAAATCGGAACCGAAAC+CGG | - | chr3.1:41208299-41208318 | MS.gene064563:CDS | 50.0% | |
| GGAGGAGAAACGGCTGAAAT+CGG | - | chr3.1:41208287-41208306 | MS.gene064563:CDS | 50.0% | |
| GTTGTCAAGTGTACCCATCC+TGG | - | chr3.1:41208842-41208861 | MS.gene064563:CDS | 50.0% | |
| TGCAGAGGGGTTTGAGAGTA+CGG | + | chr3.1:41208667-41208686 | None:intergenic | 50.0% | |
| ACAATACCGCCAGCACAACG+TGG | + | chr3.1:41208617-41208636 | None:intergenic | 55.0% | |
| TGGAGGAGGAGGAGGAGAAA+CGG | - | chr3.1:41208277-41208296 | MS.gene064563:CDS | 55.0% | |
| TTCATCTGTGGAGGAGGAGG+AGG | - | chr3.1:41208269-41208288 | MS.gene064563:CDS | 55.0% | |
| ATACCGCCAGCACAACGTGG+TGG | + | chr3.1:41208614-41208633 | None:intergenic | 60.0% | |
| CACTGTCCACCACGTTGTGC+TGG | - | chr3.1:41208605-41208624 | MS.gene064563:CDS | 60.0% | |
| TGTCCACCACGTTGTGCTGG+CGG | - | chr3.1:41208608-41208627 | MS.gene064563:CDS | 60.0% | |
| !! | CAGCACAACGTGGTGGACAG+TGG | + | chr3.1:41208607-41208626 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 41208258 | 41209024 | 41208258 | ID=MS.gene064563 |
| chr3.1 | mRNA | 41208258 | 41209024 | 41208258 | ID=MS.gene064563.t1;Parent=MS.gene064563 |
| chr3.1 | exon | 41208788 | 41209024 | 41208788 | ID=MS.gene064563.t1.exon1;Parent=MS.gene064563.t1 |
| chr3.1 | CDS | 41208788 | 41209024 | 41208788 | ID=cds.MS.gene064563.t1;Parent=MS.gene064563.t1 |
| chr3.1 | exon | 41208555 | 41208692 | 41208555 | ID=MS.gene064563.t1.exon2;Parent=MS.gene064563.t1 |
| chr3.1 | CDS | 41208555 | 41208692 | 41208555 | ID=cds.MS.gene064563.t1;Parent=MS.gene064563.t1 |
| chr3.1 | exon | 41208258 | 41208431 | 41208258 | ID=MS.gene064563.t1.exon3;Parent=MS.gene064563.t1 |
| chr3.1 | CDS | 41208258 | 41208431 | 41208258 | ID=cds.MS.gene064563.t1;Parent=MS.gene064563.t1 |
| Gene Sequence |
| Protein sequence |
