Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065085.t1 | XP_013470234.1 | 97.3 | 598 | 12 | 2 | 1 | 597 | 1 | 595 | 0.00E+00 | 1117.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065085.t1 | F4I9E1 | 22.9 | 568 | 394 | 14 | 25 | 579 | 43 | 579 | 1.2e-24 | 116.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065085.t1 | A0A072VR59 | 97.3 | 598 | 12 | 2 | 1 | 597 | 1 | 595 | 0.0e+00 | 1117.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051275 | MS.gene065085 | 0.804669 | 1.93E-49 | -1.69E-46 |
| MS.gene05216 | MS.gene065085 | 0.801803 | 7.58E-49 | -1.69E-46 |
| MS.gene052242 | MS.gene065085 | -0.805558 | 1.26E-49 | -1.69E-46 |
| MS.gene053340 | MS.gene065085 | 0.813432 | 2.57E-51 | -1.69E-46 |
| MS.gene053726 | MS.gene065085 | 0.81018 | 1.31E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene065085 | 0.810354 | 1.20E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene065085 | 0.830791 | 2.41E-55 | -1.69E-46 |
| MS.gene054893 | MS.gene065085 | 0.832808 | 7.65E-56 | -1.69E-46 |
| MS.gene056068 | MS.gene065085 | 0.802662 | 5.04E-49 | -1.69E-46 |
| MS.gene058429 | MS.gene065085 | 0.803635 | 3.17E-49 | -1.69E-46 |
| MS.gene060850 | MS.gene065085 | 0.821689 | 3.53E-53 | -1.69E-46 |
| MS.gene060851 | MS.gene065085 | 0.823761 | 1.16E-53 | -1.69E-46 |
| MS.gene060852 | MS.gene065085 | 0.822044 | 2.92E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065085.t1 | MTR_1g111550 | 97.324 | 598 | 12 | 2 | 1 | 597 | 1 | 595 | 0.0 | 1167 |
| MS.gene065085.t1 | MTR_1g111510 | 75.086 | 582 | 139 | 3 | 11 | 591 | 12 | 588 | 0.0 | 877 |
| MS.gene065085.t1 | MTR_1g111540 | 73.288 | 584 | 144 | 3 | 11 | 593 | 12 | 584 | 0.0 | 863 |
| MS.gene065085.t1 | MTR_1g111530 | 72.241 | 580 | 149 | 3 | 15 | 593 | 12 | 580 | 0.0 | 846 |
| MS.gene065085.t1 | MTR_7g102880 | 63.946 | 588 | 197 | 6 | 14 | 591 | 18 | 600 | 0.0 | 729 |
| MS.gene065085.t1 | MTR_1g050550 | 58.966 | 580 | 228 | 3 | 13 | 585 | 12 | 588 | 0.0 | 680 |
| MS.gene065085.t1 | MTR_1g059080 | 56.326 | 577 | 239 | 3 | 13 | 585 | 12 | 579 | 0.0 | 652 |
| MS.gene065085.t1 | MTR_2g100400 | 34.159 | 565 | 321 | 7 | 22 | 566 | 4 | 537 | 5.87e-103 | 322 |
| MS.gene065085.t1 | MTR_7g029015 | 34.872 | 585 | 334 | 11 | 11 | 557 | 1 | 576 | 1.97e-100 | 317 |
| MS.gene065085.t1 | MTR_5g090570 | 32.849 | 551 | 334 | 5 | 25 | 567 | 11 | 533 | 5.01e-97 | 306 |
| MS.gene065085.t1 | MTR_3g099150 | 34.650 | 557 | 324 | 12 | 25 | 558 | 15 | 554 | 2.21e-92 | 295 |
| MS.gene065085.t1 | MTR_4g073690 | 35.609 | 542 | 319 | 10 | 25 | 558 | 15 | 534 | 2.64e-87 | 281 |
| MS.gene065085.t1 | MTR_5g022380 | 32.000 | 550 | 335 | 7 | 25 | 569 | 13 | 528 | 1.34e-80 | 263 |
| MS.gene065085.t1 | MTR_2g450710 | 31.341 | 552 | 349 | 9 | 26 | 558 | 13 | 553 | 5.35e-75 | 249 |
| MS.gene065085.t1 | MTR_4g116210 | 30.357 | 560 | 351 | 11 | 26 | 558 | 12 | 559 | 5.94e-75 | 249 |
| MS.gene065085.t1 | MTR_5g022380 | 31.858 | 452 | 269 | 7 | 123 | 569 | 1 | 418 | 1.56e-59 | 204 |
| MS.gene065085.t1 | MTR_4g073690 | 35.128 | 390 | 232 | 8 | 25 | 406 | 15 | 391 | 5.19e-58 | 199 |
| MS.gene065085.t1 | MTR_5g095240 | 84.507 | 71 | 11 | 0 | 494 | 564 | 2 | 72 | 1.65e-34 | 125 |
| MS.gene065085.t1 | MTR_2g104250 | 33.624 | 229 | 109 | 5 | 330 | 558 | 104 | 289 | 4.18e-32 | 126 |
| MS.gene065085.t1 | MTR_3g466040 | 26.838 | 544 | 357 | 13 | 22 | 558 | 4 | 513 | 7.13e-28 | 118 |
| MS.gene065085.t1 | MTR_7g083660 | 24.956 | 569 | 388 | 16 | 25 | 569 | 7 | 560 | 1.51e-27 | 117 |
| MS.gene065085.t1 | MTR_3g466110 | 26.419 | 511 | 336 | 12 | 23 | 527 | 5 | 481 | 4.48e-26 | 112 |
| MS.gene065085.t1 | MTR_5g026350 | 25.391 | 575 | 380 | 15 | 25 | 566 | 7 | 565 | 2.07e-23 | 104 |
| MS.gene065085.t1 | MTR_8g071920 | 23.232 | 594 | 410 | 14 | 11 | 572 | 34 | 613 | 1.92e-20 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065085.t1 | AT2G39210 | 68.165 | 556 | 175 | 2 | 15 | 568 | 11 | 566 | 0.0 | 777 |
| MS.gene065085.t1 | AT2G28120 | 60.276 | 579 | 222 | 4 | 12 | 587 | 4 | 577 | 0.0 | 692 |
| MS.gene065085.t1 | AT1G74780 | 36.713 | 572 | 301 | 9 | 19 | 568 | 1 | 533 | 6.89e-110 | 340 |
| MS.gene065085.t1 | AT1G18940 | 34.177 | 553 | 310 | 6 | 20 | 558 | 4 | 516 | 5.92e-102 | 319 |
| MS.gene065085.t1 | AT5G14120 | 33.901 | 587 | 347 | 9 | 12 | 567 | 3 | 579 | 5.42e-101 | 318 |
| MS.gene065085.t1 | AT3G01930 | 32.598 | 589 | 353 | 8 | 14 | 567 | 5 | 584 | 1.51e-97 | 309 |
| MS.gene065085.t1 | AT2G34355 | 34.875 | 562 | 308 | 10 | 19 | 564 | 1 | 520 | 4.50e-97 | 306 |
| MS.gene065085.t1 | AT5G50520 | 34.369 | 547 | 321 | 9 | 21 | 557 | 12 | 530 | 1.83e-96 | 305 |
| MS.gene065085.t1 | AT5G50630 | 34.369 | 547 | 321 | 9 | 21 | 557 | 12 | 530 | 1.83e-96 | 305 |
| MS.gene065085.t1 | AT2G34350 | 33.932 | 557 | 310 | 7 | 25 | 564 | 7 | 522 | 5.95e-96 | 303 |
| MS.gene065085.t1 | AT2G34350 | 33.932 | 557 | 310 | 7 | 25 | 564 | 7 | 522 | 5.95e-96 | 303 |
| MS.gene065085.t1 | AT4G34950 | 33.979 | 568 | 341 | 7 | 25 | 576 | 16 | 565 | 3.05e-95 | 303 |
| MS.gene065085.t1 | AT2G16660 | 35.754 | 537 | 320 | 6 | 25 | 557 | 14 | 529 | 6.41e-91 | 291 |
| MS.gene065085.t1 | AT3G01930 | 31.250 | 480 | 286 | 8 | 123 | 567 | 1 | 471 | 4.33e-69 | 231 |
| MS.gene065085.t1 | AT1G80530 | 28.881 | 554 | 350 | 11 | 26 | 558 | 14 | 544 | 6.42e-67 | 228 |
| MS.gene065085.t1 | AT3G01930 | 32.037 | 437 | 256 | 6 | 14 | 415 | 5 | 435 | 8.10e-64 | 216 |
| MS.gene065085.t1 | AT2G16660 | 35.567 | 388 | 234 | 5 | 25 | 408 | 14 | 389 | 2.07e-59 | 203 |
| MS.gene065085.t1 | AT2G34355 | 33.333 | 411 | 224 | 8 | 162 | 564 | 2 | 370 | 2.32e-59 | 202 |
| MS.gene065085.t1 | AT3G01630 | 23.921 | 556 | 367 | 12 | 25 | 558 | 41 | 562 | 4.32e-28 | 119 |
| MS.gene065085.t1 | AT3G01630 | 23.921 | 556 | 367 | 12 | 25 | 558 | 23 | 544 | 4.97e-28 | 119 |
| MS.gene065085.t1 | AT4G19450 | 25.458 | 546 | 351 | 15 | 25 | 533 | 7 | 533 | 1.66e-24 | 108 |
| MS.gene065085.t1 | AT1G31470 | 22.456 | 570 | 394 | 13 | 25 | 579 | 43 | 579 | 2.04e-24 | 108 |
| MS.gene065085.t1 | AT5G45275 | 24.180 | 579 | 384 | 17 | 25 | 570 | 7 | 563 | 9.44e-23 | 102 |
| MS.gene065085.t1 | AT2G30300 | 26.391 | 557 | 319 | 18 | 26 | 558 | 8 | 497 | 2.86e-21 | 97.8 |
| MS.gene065085.t1 | AT4G19450 | 26.173 | 405 | 267 | 9 | 25 | 401 | 7 | 407 | 2.06e-20 | 94.4 |
Find 106 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCAAATAGCTCCGAAATTA+TGG | 0.179888 | 1.2:-82589365 | None:intergenic |
| GTCCTCTCTTGCCGTCATTT+TGG | 0.200061 | 1.2:-82589729 | None:intergenic |
| TTGTTTCATTAGTGAGTATT+TGG | 0.206900 | 1.2:+82589137 | MS.gene065085:CDS |
| GCCATAATTTCGGAGCTATT+TGG | 0.234809 | 1.2:+82589364 | MS.gene065085:CDS |
| ATTGATAATTTAGGCCAAAT+TGG | 0.239998 | 1.2:+82589076 | MS.gene065085:CDS |
| AAAAGGAGTACAAAGAAAAT+TGG | 0.243039 | 1.2:+82589510 | MS.gene065085:CDS |
| AACACAACACCCCGAGTTTC+CGG | 0.275488 | 1.2:-82588431 | None:intergenic |
| ATCACTGCAGCTACTTTGTT+TGG | 0.283099 | 1.2:+82589586 | MS.gene065085:CDS |
| ATAAGCAAAATCAAAGCTTT+TGG | 0.288180 | 1.2:-82588530 | None:intergenic |
| AAGAGGCAATTGATTGAAAA+AGG | 0.294262 | 1.2:+82589493 | MS.gene065085:CDS |
| TTAACAGCAATTGATAATTT+AGG | 0.309990 | 1.2:+82589067 | MS.gene065085:CDS |
| GAAACTCGGGGTGTTGTGTT+GGG | 0.317245 | 1.2:+82588434 | MS.gene065085:CDS |
| TTTCTAATCATGTCAGCTTC+AGG | 0.321020 | 1.2:+82588080 | MS.gene065085:CDS |
| CCATAATTTCGGAGCTATTT+GGG | 0.325331 | 1.2:+82589365 | MS.gene065085:CDS |
| CCTTATGTTGTTCTTGCAAT+TGG | 0.327850 | 1.2:+82588248 | MS.gene065085:CDS |
| AGACACATTTGCCAAACTTT+TGG | 0.333591 | 1.2:-82588335 | None:intergenic |
| ATGTGTCTTTACATTTGTAT+AGG | 0.337463 | 1.2:+82588350 | MS.gene065085:CDS |
| AAAGGTTATGTTGGTCTTAG+TGG | 0.349558 | 1.2:+82588464 | MS.gene065085:CDS |
| ATCCAAGTGAGGTTCCAATT+TGG | 0.354689 | 1.2:-82589090 | None:intergenic |
| ATGTTATTGCTATTCTTTGC+TGG | 0.355250 | 1.2:+82589022 | MS.gene065085:CDS |
| GTTAAGAGTTGTTTGGTCAT+AGG | 0.356138 | 1.2:-82588153 | None:intergenic |
| CACTAAAATAAGTGAAATAA+TGG | 0.361527 | 1.2:-82589611 | None:intergenic |
| AAATACCAAAACCAAAAGTT+TGG | 0.367844 | 1.2:+82588324 | MS.gene065085:CDS |
| ACACAACACCCCGAGTTTCC+GGG | 0.369260 | 1.2:-82588430 | None:intergenic |
| GTAAAATCTTCACCTTGTTC+AGG | 0.370366 | 1.2:-82588971 | None:intergenic |
| CTGTGATAACTTGGATTATG+AGG | 0.371769 | 1.2:-82588031 | None:intergenic |
| ATAGCCTTTGATGTGCAAAA+TGG | 0.396133 | 1.2:+82589280 | MS.gene065085:CDS |
| GGAAACTCGGGGTGTTGTGT+TGG | 0.398139 | 1.2:+82588433 | MS.gene065085:CDS |
| TTTCTTAATGTTAGGGTCGC+TGG | 0.402287 | 1.2:+82589448 | MS.gene065085:CDS |
| TATATTTCGCTTGGTCTAGC+TGG | 0.405217 | 1.2:+82588656 | MS.gene065085:CDS |
| TATTCAACTTTGATGAACTT+TGG | 0.405251 | 1.2:+82589397 | MS.gene065085:CDS |
| TCAGGAGCCACCTACATGTT+TGG | 0.407429 | 1.2:+82588098 | MS.gene065085:CDS |
| ATAACATATCTATGCTGAAA+AGG | 0.416968 | 1.2:-82589006 | None:intergenic |
| CTTAGTTTCTTCAAAGACTT+AGG | 0.418305 | 1.2:+82588179 | MS.gene065085:CDS |
| TTTAAACAGAGTGAGTATAT+TGG | 0.420191 | 1.2:+82588719 | MS.gene065085:CDS |
| AGTTCGTTGTTCTGTTTAAC+CGG | 0.420863 | 1.2:-82588611 | None:intergenic |
| GGTGGGGTAGCTAGTCCAAT+TGG | 0.428284 | 1.2:+82589418 | MS.gene065085:CDS |
| ACCATCGTCCTGTGATAACT+TGG | 0.432297 | 1.2:-82588040 | None:intergenic |
| TAAGGAGGTTAAGAGTTGTT+TGG | 0.437311 | 1.2:-82588160 | None:intergenic |
| TTAAGAAAATATAATCCAAT+TGG | 0.438575 | 1.2:-82589433 | None:intergenic |
| GGACAGGAAGCAAAAGCTAC+AGG | 0.443209 | 1.2:+82589748 | MS.gene065085:CDS |
| GGCCAAATTGGAACCTCACT+TGG | 0.443606 | 1.2:+82589088 | MS.gene065085:CDS |
| AAAGGAGTACAAAGAAAATT+GGG | 0.445150 | 1.2:+82589511 | MS.gene065085:CDS |
| CTAAGTCTTTGAAGAAACTA+AGG | 0.454900 | 1.2:-82588178 | None:intergenic |
| CAACTTTGATGAACTTTGGT+GGG | 0.470861 | 1.2:+82589401 | MS.gene065085:CDS |
| TAACATATCTATGCTGAAAA+GGG | 0.471235 | 1.2:-82589005 | None:intergenic |
| TTGTTTGAGATAACCTCAAC+GGG | 0.472230 | 1.2:-82588004 | None:intergenic |
| TTTGCTGGCACATGTGGTGT+TGG | 0.472238 | 1.2:+82589037 | MS.gene065085:CDS |
| AAAAGAAAGGAAATTGCTGC+TGG | 0.480135 | 1.2:-82588563 | None:intergenic |
| TCAGAGCAGGTTGAAGTTGA+AGG | 0.480550 | 1.2:+82589682 | MS.gene065085:CDS |
| AACACAAGTAACAAGTGAAC+CGG | 0.486587 | 1.2:-82588399 | None:intergenic |
| AAACTCGGGGTGTTGTGTTG+GGG | 0.490414 | 1.2:+82588435 | MS.gene065085:CDS |
| CTATTCTTTGCTGGCACATG+TGG | 0.497313 | 1.2:+82589031 | MS.gene065085:CDS |
| GCTGGCACATGTGGTGTTGG+TGG | 0.499413 | 1.2:+82589040 | MS.gene065085:CDS |
| GACACCAAAATGGCGGTTGA+AGG | 0.501902 | 1.2:+82587975 | None:intergenic |
| TTAAGAATTTCCCGGAAACT+CGG | 0.505956 | 1.2:+82588420 | MS.gene065085:CDS |
| TAAGAATTTCCCGGAAACTC+GGG | 0.508351 | 1.2:+82588421 | MS.gene065085:CDS |
| TATATGACAAAGAAGCTAAG+AGG | 0.509800 | 1.2:+82589476 | MS.gene065085:CDS |
| AGTTGTTTGGTCATAGGCTA+AGG | 0.514651 | 1.2:-82588147 | None:intergenic |
| AACCATTCGATACATGAAAC+CGG | 0.515497 | 1.2:+82588592 | MS.gene065085:CDS |
| TACTTGTGTTAAGAATTTCC+CGG | 0.516653 | 1.2:+82588412 | MS.gene065085:CDS |
| AACCGGTTTCATGTATCGAA+TGG | 0.520822 | 1.2:-82588594 | None:intergenic |
| AGTACAAAGAAAATTGGGTC+AGG | 0.524550 | 1.2:+82589516 | MS.gene065085:CDS |
| CCAATTGCAAGAACAACATA+AGG | 0.528707 | 1.2:-82588248 | None:intergenic |
| GTTAAAATAGTAACTGATCA+AGG | 0.530498 | 1.2:+82588851 | MS.gene065085:CDS |
| GTTAAACAGAACAACGAACT+TGG | 0.531720 | 1.2:+82588614 | MS.gene065085:CDS |
| GAAATTATGGCGAAAACCAA+TGG | 0.538856 | 1.2:-82589352 | None:intergenic |
| TCAACTTTGATGAACTTTGG+TGG | 0.540207 | 1.2:+82589400 | MS.gene065085:CDS |
| TACATGTTTGGTCTTTACTC+CGG | 0.541203 | 1.2:+82588110 | MS.gene065085:CDS |
| TGATTACTGAAGCAAAATAG+AGG | 0.545938 | 1.2:-82589303 | None:intergenic |
| AAATCTTCACCTTGTTCAGG+TGG | 0.547378 | 1.2:-82588968 | None:intergenic |
| AAAGACTTAGGTGGCAACCT+TGG | 0.554717 | 1.2:+82588191 | MS.gene065085:CDS |
| AAATGACGGCAAGAGAGGAC+AGG | 0.559480 | 1.2:+82589732 | MS.gene065085:CDS |
| GTGAGTATTTGGAATTACCT+AGG | 0.563575 | 1.2:+82589148 | MS.gene065085:CDS |
| TATCACAGGACGATGGTTCG+TGG | 0.571165 | 1.2:+82588046 | MS.gene065085:CDS |
| CTCATAATCCAAGTTATCAC+AGG | 0.573175 | 1.2:+82588032 | MS.gene065085:CDS |
| CAAAGACAACGGCAAGAGGT+AGG | 0.574047 | 1.2:-82588772 | None:intergenic |
| GTCCAAAATGACGGCAAGAG+AGG | 0.575323 | 1.2:+82589727 | MS.gene065085:CDS |
| CAAGAGGTACCGATCAGAGC+AGG | 0.579521 | 1.2:+82589669 | MS.gene065085:CDS |
| AGGAGCTACGGCTGAGATGA+CGG | 0.582623 | 1.2:+82589702 | MS.gene065085:CDS |
| AGTTTCTTCAAAGACTTAGG+TGG | 0.582691 | 1.2:+82588182 | MS.gene065085:CDS |
| AACTTTGATGAACTTTGGTG+GGG | 0.586311 | 1.2:+82589402 | MS.gene065085:CDS |
| AAATTGTGATACTAATGCAA+TGG | 0.591899 | 1.2:+82588895 | MS.gene065085:CDS |
| CAACTTCAACCTGCTCTGAT+CGG | 0.592162 | 1.2:-82589678 | None:intergenic |
| ATGACGGTGGTCCAAAATGA+CGG | 0.592384 | 1.2:+82589718 | MS.gene065085:CDS |
| GGTTGAAGTTGAAGGAGCTA+CGG | 0.593890 | 1.2:+82589690 | MS.gene065085:CDS |
| AATGTGAACATGAGTGGTCG+TGG | 0.597649 | 1.2:-82589223 | None:intergenic |
| GGTCAGGAATTGAATTGTGT+AGG | 0.604873 | 1.2:+82589532 | MS.gene065085:CDS |
| TATCAATAAGTGACCGACAC+AGG | 0.607344 | 1.2:-82589260 | None:intergenic |
| AAGAATTTCCCGGAAACTCG+GGG | 0.607797 | 1.2:+82588422 | MS.gene065085:CDS |
| GACAGGAAGCAAAAGCTACA+GGG | 0.610641 | 1.2:+82589749 | MS.gene065085:CDS |
| TTTGTTTGAGATAACCTCAA+CGG | 0.614309 | 1.2:-82588005 | None:intergenic |
| AATCTTCACCTTGTTCAGGT+GGG | 0.618597 | 1.2:-82588967 | None:intergenic |
| TTGAAGGTGTAAGCCCGTTG+AGG | 0.620655 | 1.2:+82587991 | MS.gene065085:CDS |
| AGTCTTTGAAGAAACTAAGG+AGG | 0.622224 | 1.2:-82588175 | None:intergenic |
| ATAATCAATGTGAACATGAG+TGG | 0.627737 | 1.2:-82589229 | None:intergenic |
| AAAGAAAGGAAATTGCTGCT+GGG | 0.629241 | 1.2:-82588562 | None:intergenic |
| TCAACAAAGACAACGGCAAG+AGG | 0.636863 | 1.2:-82588776 | None:intergenic |
| GGAGTAAAGACCAAACATGT+AGG | 0.637378 | 1.2:-82588108 | None:intergenic |
| TTTCTGTTCAACAAAGACAA+CGG | 0.650362 | 1.2:-82588783 | None:intergenic |
| ATAAAGGTGATATATACAAG+AGG | 0.658630 | 1.2:+82589653 | MS.gene065085:CDS |
| GTAAAGACCAAACATGTAGG+TGG | 0.688543 | 1.2:-82588105 | None:intergenic |
| TCCAAGTTATCACAGGACGA+TGG | 0.698322 | 1.2:+82588039 | MS.gene065085:CDS |
| ATTGCAAGAACAACATAAGG+CGG | 0.705972 | 1.2:-82588245 | None:intergenic |
| AGCTACGGCTGAGATGACGG+TGG | 0.773314 | 1.2:+82589705 | MS.gene065085:CDS |
| TTAAGCCAGAGAAAACACCA+AGG | 0.797705 | 1.2:-82588208 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATATTTTCTTAATGTTA+GGG | + | chr1.2:82589441-82589460 | MS.gene065085:CDS | 10.0% |
| !! | TTAAGAAAATATAATCCAAT+TGG | - | chr1.2:82589436-82589455 | None:intergenic | 15.0% |
| !!! | AGAACAATTAAGTTTTATAA+AGG | + | chr1.2:82589637-82589656 | MS.gene065085:CDS | 15.0% |
| !!! | GATTATATTTTCTTAATGTT+AGG | + | chr1.2:82589440-82589459 | MS.gene065085:CDS | 15.0% |
| !! | AGTAACCAAAAAAATTAAGA+AGG | - | chr1.2:82588277-82588296 | None:intergenic | 20.0% |
| !! | CACTAAAATAAGTGAAATAA+TGG | - | chr1.2:82589614-82589633 | None:intergenic | 20.0% |
| !! | GTAACCAAAAAAATTAAGAA+GGG | - | chr1.2:82588276-82588295 | None:intergenic | 20.0% |
| !!! | CATTATTTCACTTATTTTAG+TGG | + | chr1.2:82589612-82589631 | MS.gene065085:CDS | 20.0% |
| !!! | TTAACAGCAATTGATAATTT+AGG | + | chr1.2:82589067-82589086 | MS.gene065085:CDS | 20.0% |
| !!! | TTTTGGTTTTGGTATTTTTT+TGG | - | chr1.2:82588321-82588340 | None:intergenic | 20.0% |
| ! | AAATACCAAAACCAAAAGTT+TGG | + | chr1.2:82588324-82588343 | MS.gene065085:CDS | 25.0% |
| ! | AAATTGTGATACTAATGCAA+TGG | + | chr1.2:82588895-82588914 | MS.gene065085:CDS | 25.0% |
| ! | AATGGTTCGAATAAAAAGAA+AGG | - | chr1.2:82588579-82588598 | None:intergenic | 25.0% |
| ! | ATAAAGGTGATATATACAAG+AGG | + | chr1.2:82589653-82589672 | MS.gene065085:CDS | 25.0% |
| ! | ATAACATATCTATGCTGAAA+AGG | - | chr1.2:82589009-82589028 | None:intergenic | 25.0% |
| ! | ATGTGTCTTTACATTTGTAT+AGG | + | chr1.2:82588350-82588369 | MS.gene065085:CDS | 25.0% |
| ! | GTTAAAATAGTAACTGATCA+AGG | + | chr1.2:82588851-82588870 | MS.gene065085:CDS | 25.0% |
| ! | TAACATATCTATGCTGAAAA+GGG | - | chr1.2:82589008-82589027 | None:intergenic | 25.0% |
| ! | TATTCAACTTTGATGAACTT+TGG | + | chr1.2:82589397-82589416 | MS.gene065085:CDS | 25.0% |
| ! | TTGAAGAAAAAGAAACTAGA+TGG | + | chr1.2:82588927-82588946 | MS.gene065085:CDS | 25.0% |
| ! | TTGTTTCATTAGTGAGTATT+TGG | + | chr1.2:82589137-82589156 | MS.gene065085:CDS | 25.0% |
| ! | TTTAAACAGAGTGAGTATAT+TGG | + | chr1.2:82588719-82588738 | MS.gene065085:CDS | 25.0% |
| !! | AAAAGGAGTACAAAGAAAAT+TGG | + | chr1.2:82589510-82589529 | MS.gene065085:CDS | 25.0% |
| !! | AAAGGAGTACAAAGAAAATT+GGG | + | chr1.2:82589511-82589530 | MS.gene065085:CDS | 25.0% |
| !! | AAGTTTTTGTATATTTCGCT+TGG | + | chr1.2:82588647-82588666 | MS.gene065085:CDS | 25.0% |
| !! | ATAAGCAAAATCAAAGCTTT+TGG | - | chr1.2:82588533-82588552 | None:intergenic | 25.0% |
| !! | ATTGATAATTTAGGCCAAAT+TGG | + | chr1.2:82589076-82589095 | MS.gene065085:CDS | 25.0% |
| !! | TATTTTGCTTCAGTAATCAT+TGG | + | chr1.2:82589307-82589326 | MS.gene065085:CDS | 25.0% |
| !!! | ATTATGTTTTTATCCTGTGT+CGG | + | chr1.2:82589247-82589266 | MS.gene065085:CDS | 25.0% |
| !!! | GTAATCATTGGATTTTGTTT+TGG | + | chr1.2:82589319-82589338 | MS.gene065085:CDS | 25.0% |
| !!! | GTTACTATTTTAACCGAAAA+TGG | - | chr1.2:82588845-82588864 | None:intergenic | 25.0% |
| !!! | TTTTTGGTTACTTCATGATA+TGG | + | chr1.2:82588285-82588304 | MS.gene065085:CDS | 25.0% |
| AAGAAAAAGAAACTAGATGG+TGG | + | chr1.2:82588930-82588949 | MS.gene065085:CDS | 30.0% | |
| AAGAGGCAATTGATTGAAAA+AGG | + | chr1.2:82589493-82589512 | MS.gene065085:CDS | 30.0% | |
| ATAATCAATGTGAACATGAG+TGG | - | chr1.2:82589232-82589251 | None:intergenic | 30.0% | |
| CTTAGTTTCTTCAAAGACTT+AGG | + | chr1.2:82588179-82588198 | MS.gene065085:CDS | 30.0% | |
| TACTTGTGTTAAGAATTTCC+CGG | + | chr1.2:82588412-82588431 | MS.gene065085:CDS | 30.0% | |
| TATATGACAAAGAAGCTAAG+AGG | + | chr1.2:82589476-82589495 | MS.gene065085:CDS | 30.0% | |
| TGATTACTGAAGCAAAATAG+AGG | - | chr1.2:82589306-82589325 | None:intergenic | 30.0% | |
| TTTCTGTTCAACAAAGACAA+CGG | - | chr1.2:82588786-82588805 | None:intergenic | 30.0% | |
| ! | ATGTTATTGCTATTCTTTGC+TGG | + | chr1.2:82589022-82589041 | MS.gene065085:CDS | 30.0% |
| ! | CTAAGTCTTTGAAGAAACTA+AGG | - | chr1.2:82588181-82588200 | None:intergenic | 30.0% |
| ! | TTTGTTTGAGATAACCTCAA+CGG | - | chr1.2:82588008-82588027 | None:intergenic | 30.0% |
| !! | AATGTGCTTATGCTTGTTTT+TGG | - | chr1.2:82589118-82589137 | None:intergenic | 30.0% |
| !! | ATAGGCTAAGGATGTTTTTA+TGG | - | chr1.2:82588138-82588157 | None:intergenic | 30.0% |
| !! | ATGTGCTTATGCTTGTTTTT+GGG | - | chr1.2:82589117-82589136 | None:intergenic | 30.0% |
| !! | CTTTGATTTTGCTTATTGCT+TGG | + | chr1.2:82588537-82588556 | MS.gene065085:CDS | 30.0% |
| !! | GGGATTTTAAAAGGTTATGT+TGG | + | chr1.2:82588455-82588474 | MS.gene065085:CDS | 30.0% |
| !! | TAAGGATGTTTTTATGGTAC+CGG | - | chr1.2:82588132-82588151 | None:intergenic | 30.0% |
| !! | TGCATTTGTTGATCCATTTT+CGG | + | chr1.2:82588829-82588848 | MS.gene065085:CDS | 30.0% |
| !!! | ATTGGTTTTCGCCATAATTT+CGG | + | chr1.2:82589354-82589373 | MS.gene065085:CDS | 30.0% |
| !!! | ATTTGCCAAACTTTTGGTTT+TGG | - | chr1.2:82588332-82588351 | None:intergenic | 30.0% |
| !!! | GGTTCCCTTCTTAATTTTTT+TGG | + | chr1.2:82588269-82588288 | MS.gene065085:CDS | 30.0% |
| AAAAGAAAGGAAATTGCTGC+TGG | - | chr1.2:82588566-82588585 | None:intergenic | 35.0% | |
| AAAGAAAGGAAATTGCTGCT+GGG | - | chr1.2:82588565-82588584 | None:intergenic | 35.0% | |
| AACACAAGTAACAAGTGAAC+CGG | - | chr1.2:82588402-82588421 | None:intergenic | 35.0% | |
| AACCAGAAAAAACTCTTCCT+AGG | - | chr1.2:82589168-82589187 | None:intergenic | 35.0% | |
| AACCATTCGATACATGAAAC+CGG | + | chr1.2:82588592-82588611 | MS.gene065085:CDS | 35.0% | |
| AACTTTGATGAACTTTGGTG+GGG | + | chr1.2:82589402-82589421 | MS.gene065085:CDS | 35.0% | |
| AGTACAAAGAAAATTGGGTC+AGG | + | chr1.2:82589516-82589535 | MS.gene065085:CDS | 35.0% | |
| AGTTCGTTGTTCTGTTTAAC+CGG | - | chr1.2:82588614-82588633 | None:intergenic | 35.0% | |
| AGTTTCTTCAAAGACTTAGG+TGG | + | chr1.2:82588182-82588201 | MS.gene065085:CDS | 35.0% | |
| ATAGCCTTTGATGTGCAAAA+TGG | + | chr1.2:82589280-82589299 | MS.gene065085:CDS | 35.0% | |
| ATTGCAAGAACAACATAAGG+CGG | - | chr1.2:82588248-82588267 | None:intergenic | 35.0% | |
| CAACTTTGATGAACTTTGGT+GGG | + | chr1.2:82589401-82589420 | MS.gene065085:CDS | 35.0% | |
| CCAATTGCAAGAACAACATA+AGG | - | chr1.2:82588251-82588270 | None:intergenic | 35.0% | |
| CCATAATTTCGGAGCTATTT+GGG | + | chr1.2:82589365-82589384 | MS.gene065085:CDS | 35.0% | |
| CCTTATGTTGTTCTTGCAAT+TGG | + | chr1.2:82588248-82588267 | MS.gene065085:CDS | 35.0% | |
| CTCATAATCCAAGTTATCAC+AGG | + | chr1.2:82588032-82588051 | MS.gene065085:CDS | 35.0% | |
| CTGTGATAACTTGGATTATG+AGG | - | chr1.2:82588034-82588053 | None:intergenic | 35.0% | |
| GAAATTATGGCGAAAACCAA+TGG | - | chr1.2:82589355-82589374 | None:intergenic | 35.0% | |
| GTAAAATCTTCACCTTGTTC+AGG | - | chr1.2:82588974-82588993 | None:intergenic | 35.0% | |
| GTTAAACAGAACAACGAACT+TGG | + | chr1.2:82588614-82588633 | MS.gene065085:CDS | 35.0% | |
| GTTAAGAGTTGTTTGGTCAT+AGG | - | chr1.2:82588156-82588175 | None:intergenic | 35.0% | |
| TAAGGAGGTTAAGAGTTGTT+TGG | - | chr1.2:82588163-82588182 | None:intergenic | 35.0% | |
| TCAACTTTGATGAACTTTGG+TGG | + | chr1.2:82589400-82589419 | MS.gene065085:CDS | 35.0% | |
| TTAAGAATTTCCCGGAAACT+CGG | + | chr1.2:82588420-82588439 | MS.gene065085:CDS | 35.0% | |
| TTTCTAATCATGTCAGCTTC+AGG | + | chr1.2:82588080-82588099 | MS.gene065085:CDS | 35.0% | |
| ! | AGACACATTTGCCAAACTTT+TGG | - | chr1.2:82588338-82588357 | None:intergenic | 35.0% |
| ! | AGTCTTTGAAGAAACTAAGG+AGG | - | chr1.2:82588178-82588197 | None:intergenic | 35.0% |
| ! | GTGAGTATTTGGAATTACCT+AGG | + | chr1.2:82589148-82589167 | MS.gene065085:CDS | 35.0% |
| ! | GTTGTGTTGGGGATTTTAAA+AGG | + | chr1.2:82588446-82588465 | MS.gene065085:CDS | 35.0% |
| ! | TACATGTTTGGTCTTTACTC+CGG | + | chr1.2:82588110-82588129 | MS.gene065085:CDS | 35.0% |
| ! | TTGTTTGAGATAACCTCAAC+GGG | - | chr1.2:82588007-82588026 | None:intergenic | 35.0% |
| !! | AAAGGTTATGTTGGTCTTAG+TGG | + | chr1.2:82588464-82588483 | MS.gene065085:CDS | 35.0% |
| !!! | TACCTAGGAAGAGTTTTTTC+TGG | + | chr1.2:82589163-82589182 | MS.gene065085:CDS | 35.0% |
| !!! | TCTCAGTCTTTTTCAAACAC+CGG | + | chr1.2:82588380-82588399 | MS.gene065085:CDS | 35.0% |
| !!! | TGGTCTAGCTGGTTTTTTAA+TGG | + | chr1.2:82588667-82588686 | MS.gene065085:CDS | 35.0% |
| AAATCTTCACCTTGTTCAGG+TGG | - | chr1.2:82588971-82588990 | None:intergenic | 40.0% | |
| AATCTTCACCTTGTTCAGGT+GGG | - | chr1.2:82588970-82588989 | None:intergenic | 40.0% | |
| AGTTGTTTGGTCATAGGCTA+AGG | - | chr1.2:82588150-82588169 | None:intergenic | 40.0% | |
| ATCCAAGTGAGGTTCCAATT+TGG | - | chr1.2:82589093-82589112 | None:intergenic | 40.0% | |
| CCCAAATAGCTCCGAAATTA+TGG | - | chr1.2:82589368-82589387 | None:intergenic | 40.0% | |
| GCCATAATTTCGGAGCTATT+TGG | + | chr1.2:82589364-82589383 | MS.gene065085:CDS | 40.0% | |
| GGAGTAAAGACCAAACATGT+AGG | - | chr1.2:82588111-82588130 | None:intergenic | 40.0% | |
| GGTCAGGAATTGAATTGTGT+AGG | + | chr1.2:82589532-82589551 | MS.gene065085:CDS | 40.0% | |
| GTAAAGACCAAACATGTAGG+TGG | - | chr1.2:82588108-82588127 | None:intergenic | 40.0% | |
| TAAGAATTTCCCGGAAACTC+GGG | + | chr1.2:82588421-82588440 | MS.gene065085:CDS | 40.0% | |
| TATATTTCGCTTGGTCTAGC+TGG | + | chr1.2:82588656-82588675 | MS.gene065085:CDS | 40.0% | |
| TATCAATAAGTGACCGACAC+AGG | - | chr1.2:82589263-82589282 | None:intergenic | 40.0% | |
| TTAAGCCAGAGAAAACACCA+AGG | - | chr1.2:82588211-82588230 | None:intergenic | 40.0% | |
| TTTCTTAATGTTAGGGTCGC+TGG | + | chr1.2:82589448-82589467 | MS.gene065085:CDS | 40.0% | |
| ! | AACCGGTTTCATGTATCGAA+TGG | - | chr1.2:82588597-82588616 | None:intergenic | 40.0% |
| ! | ATCACTGCAGCTACTTTGTT+TGG | + | chr1.2:82589586-82589605 | MS.gene065085:CDS | 40.0% |
| !!! | ATTTTTAGCCCACCTGAACA+AGG | + | chr1.2:82588959-82588978 | MS.gene065085:CDS | 40.0% |
| !!! | GATTTTGTTTTGGTGCACAG+TGG | + | chr1.2:82589329-82589348 | MS.gene065085:CDS | 40.0% |
| !!! | TGTTTTTGGGTATCCAAGTG+AGG | - | chr1.2:82589104-82589123 | None:intergenic | 40.0% |
| AAAGACTTAGGTGGCAACCT+TGG | + | chr1.2:82588191-82588210 | MS.gene065085:CDS | 45.0% | |
| AAGAATTTCCCGGAAACTCG+GGG | + | chr1.2:82588422-82588441 | MS.gene065085:CDS | 45.0% | |
| AATGTGAACATGAGTGGTCG+TGG | - | chr1.2:82589226-82589245 | None:intergenic | 45.0% | |
| ACCATCGTCCTGTGATAACT+TGG | - | chr1.2:82588043-82588062 | None:intergenic | 45.0% | |
| ATGACGGTGGTCCAAAATGA+CGG | + | chr1.2:82589718-82589737 | MS.gene065085:CDS | 45.0% | |
| CAACTTCAACCTGCTCTGAT+CGG | - | chr1.2:82589681-82589700 | None:intergenic | 45.0% | |
| CTATTCTTTGCTGGCACATG+TGG | + | chr1.2:82589031-82589050 | MS.gene065085:CDS | 45.0% | |
| GACAGGAAGCAAAAGCTACA+GGG | + | chr1.2:82589749-82589768 | MS.gene065085:CDS | 45.0% | |
| GGTTGAAGTTGAAGGAGCTA+CGG | + | chr1.2:82589690-82589709 | MS.gene065085:CDS | 45.0% | |
| TCAACAAAGACAACGGCAAG+AGG | - | chr1.2:82588779-82588798 | None:intergenic | 45.0% | |
| TCCAAGTTATCACAGGACGA+TGG | + | chr1.2:82588039-82588058 | MS.gene065085:CDS | 45.0% | |
| ! | TCAGAGCAGGTTGAAGTTGA+AGG | + | chr1.2:82589682-82589701 | MS.gene065085:CDS | 45.0% |
| !! | GAGGCCATTTTGCACATCAA+AGG | - | chr1.2:82589287-82589306 | None:intergenic | 45.0% |
| AAATGACGGCAAGAGAGGAC+AGG | + | chr1.2:82589732-82589751 | MS.gene065085:CDS | 50.0% | |
| AACACAACACCCCGAGTTTC+CGG | - | chr1.2:82588434-82588453 | None:intergenic | 50.0% | |
| CAAAGACAACGGCAAGAGGT+AGG | - | chr1.2:82588775-82588794 | None:intergenic | 50.0% | |
| GGACAGGAAGCAAAAGCTAC+AGG | + | chr1.2:82589748-82589767 | MS.gene065085:CDS | 50.0% | |
| GGCCAAATTGGAACCTCACT+TGG | + | chr1.2:82589088-82589107 | MS.gene065085:CDS | 50.0% | |
| GTCCAAAATGACGGCAAGAG+AGG | + | chr1.2:82589727-82589746 | MS.gene065085:CDS | 50.0% | |
| TATCACAGGACGATGGTTCG+TGG | + | chr1.2:82588046-82588065 | MS.gene065085:CDS | 50.0% | |
| TCAGGAGCCACCTACATGTT+TGG | + | chr1.2:82588098-82588117 | MS.gene065085:CDS | 50.0% | |
| TTTGCTGGCACATGTGGTGT+TGG | + | chr1.2:82589037-82589056 | MS.gene065085:CDS | 50.0% | |
| ! | GTCCTCTCTTGCCGTCATTT+TGG | - | chr1.2:82589732-82589751 | None:intergenic | 50.0% |
| ! | TTGAAGGTGTAAGCCCGTTG+AGG | + | chr1.2:82587991-82588010 | MS.gene065085:CDS | 50.0% |
| !! | AAACTCGGGGTGTTGTGTTG+GGG | + | chr1.2:82588435-82588454 | MS.gene065085:CDS | 50.0% |
| !! | GAAACTCGGGGTGTTGTGTT+GGG | + | chr1.2:82588434-82588453 | MS.gene065085:CDS | 50.0% |
| !! | TTTTGGTGCACAGTGGCCAT+TGG | + | chr1.2:82589336-82589355 | MS.gene065085:CDS | 50.0% |
| !!! | GGCAACCTTGGTGTTTTCTC+TGG | + | chr1.2:82588203-82588222 | MS.gene065085:CDS | 50.0% |
| ACACAACACCCCGAGTTTCC+GGG | - | chr1.2:82588433-82588452 | None:intergenic | 55.0% | |
| AGGAGCTACGGCTGAGATGA+CGG | + | chr1.2:82589702-82589721 | MS.gene065085:CDS | 55.0% | |
| GGTGGGGTAGCTAGTCCAAT+TGG | + | chr1.2:82589418-82589437 | MS.gene065085:CDS | 55.0% | |
| !! | CAAGAGGTACCGATCAGAGC+AGG | + | chr1.2:82589669-82589688 | MS.gene065085:CDS | 55.0% |
| !! | GGAAACTCGGGGTGTTGTGT+TGG | + | chr1.2:82588433-82588452 | MS.gene065085:CDS | 55.0% |
| ! | AGCTACGGCTGAGATGACGG+TGG | + | chr1.2:82589705-82589724 | MS.gene065085:CDS | 60.0% |
| ! | GCTGGCACATGTGGTGTTGG+TGG | + | chr1.2:82589040-82589059 | MS.gene065085:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 82587984 | 82589777 | 82587984 | ID=MS.gene065085 |
| chr1.2 | mRNA | 82587984 | 82589777 | 82587984 | ID=MS.gene065085.t1;Parent=MS.gene065085 |
| chr1.2 | exon | 82587984 | 82589777 | 82587984 | ID=MS.gene065085.t1.exon1;Parent=MS.gene065085.t1 |
| chr1.2 | CDS | 82587984 | 82589777 | 82587984 | ID=cds.MS.gene065085.t1;Parent=MS.gene065085.t1 |
| Gene Sequence |
| Protein sequence |
