Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065261.t1 | RHN75073.1 | 91.6 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 5.40E-34 | 153.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065261.t1 | A0A072VB89 | 91.6 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 3.9e-34 | 153.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049647 | MS.gene065261 | 0.80383 | 2.89E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene065261 | 0.809102 | 2.23E-50 | -1.69E-46 |
| MS.gene050719 | MS.gene065261 | 0.838078 | 3.56E-57 | -1.69E-46 |
| MS.gene050721 | MS.gene065261 | 0.834271 | 3.30E-56 | -1.69E-46 |
| MS.gene050724 | MS.gene065261 | 0.828713 | 7.71E-55 | -1.69E-46 |
| MS.gene050844 | MS.gene065261 | 0.814404 | 1.57E-51 | -1.69E-46 |
| MS.gene051265 | MS.gene065261 | 0.807201 | 5.67E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene065261 | 0.805899 | 1.07E-49 | -1.69E-46 |
| MS.gene052190 | MS.gene065261 | 0.821923 | 3.12E-53 | -1.69E-46 |
| MS.gene053221 | MS.gene065261 | 0.803222 | 3.86E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene065261 | 0.809041 | 2.30E-50 | -1.69E-46 |
| MS.gene055356 | MS.gene065261 | 0.801381 | 9.25E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene065261 | 0.805671 | 1.19E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene065261 | 0.803407 | 3.54E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene065261 | 0.817226 | 3.68E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene065261 | 0.803843 | 2.87E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene065261 | 0.802551 | 5.32E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene065261 | 0.803007 | 4.28E-49 | -1.69E-46 |
| MS.gene059128 | MS.gene065261 | 0.818585 | 1.82E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene065261 | 0.920772 | 8.38E-88 | -1.69E-46 |
| MS.gene060322 | MS.gene065261 | 0.831574 | 1.55E-55 | -1.69E-46 |
| MS.gene060572 | MS.gene065261 | 0.808291 | 3.33E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065261.t1 | MTR_2g079000 | 91.566 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 4.55e-42 | 137 |
| MS.gene065261.t1 | MTR_5g012820 | 66.279 | 86 | 26 | 3 | 3 | 87 | 7 | 90 | 4.28e-24 | 93.6 |
| MS.gene065261.t1 | MTR_5g012820 | 67.949 | 78 | 23 | 2 | 11 | 87 | 4 | 80 | 6.44e-23 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065261.t1 | AT5G05210 | 57.627 | 59 | 24 | 1 | 29 | 87 | 24 | 81 | 1.91e-13 | 64.3 |
| MS.gene065261.t1 | AT5G05210 | 57.627 | 59 | 24 | 1 | 29 | 87 | 16 | 73 | 2.21e-13 | 63.9 |
Find 18 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACGAGAAGGCATGGTTTC+AGG | 0.217653 | 2.4:+19233214 | MS.gene065261:CDS |
| TTTATTACAATGAGAGATTC+CGG | 0.340961 | 2.4:+19233047 | None:intergenic |
| CAGAAGGGTGTTGCAGATAT+TGG | 0.354363 | 2.4:+19233092 | MS.gene065261:CDS |
| GTTGGTAGATAAAACTTAGC+TGG | 0.463279 | 2.4:-19233185 | None:intergenic |
| AACGAGAAGGCATGGTTTCA+GGG | 0.469679 | 2.4:+19233215 | MS.gene065261:CDS |
| TATTGGTGCTTGTCAAGACT+TGG | 0.497123 | 2.4:+19233109 | MS.gene065261:CDS |
| GTTAGGGTCTGAACTCTAAA+CGG | 0.521104 | 2.4:-19233307 | None:intergenic |
| GCCTTCTCGTTATCATCAGT+TGG | 0.521411 | 2.4:-19233203 | None:intergenic |
| AATGAGAGATTCCGGAGTGA+TGG | 0.528245 | 2.4:+19233055 | None:intergenic |
| GGAGATTAAGAAGAAGCAGA+AGG | 0.532482 | 2.4:+19233076 | MS.gene065261:CDS |
| GAGATTAAGAAGAAGCAGAA+GGG | 0.542455 | 2.4:+19233077 | MS.gene065261:CDS |
| AAGCAAGGGTGCAAAAGCTA+AGG | 0.564446 | 2.4:+19233241 | MS.gene065261:CDS |
| TAGACAATATTATGAAGTCT+CGG | 0.586642 | 2.4:+19233279 | MS.gene065261:CDS |
| ATGGTTTCAGGGTCTAAGCA+AGG | 0.591717 | 2.4:+19233226 | MS.gene065261:CDS |
| CTTCTTAATCTCCATCACTC+CGG | 0.599988 | 2.4:-19233066 | None:intergenic |
| CTGATGATAACGAGAAGGCA+TGG | 0.624704 | 2.4:+19233207 | MS.gene065261:CDS |
| TGGTTTCAGGGTCTAAGCAA+GGG | 0.633440 | 2.4:+19233227 | MS.gene065261:CDS |
| ACCAACTGATGATAACGAGA+AGG | 0.659759 | 2.4:+19233202 | MS.gene065261:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATCAATTTGTCGAAAAAAT+GGG | - | chr2.4:19233156-19233175 | None:intergenic | 25.0% |
| ! | TAGACAATATTATGAAGTCT+CGG | + | chr2.4:19233279-19233298 | MS.gene065261:CDS | 25.0% |
| CGATCAATTTGTCGAAAAAA+TGG | - | chr2.4:19233157-19233176 | None:intergenic | 30.0% | |
| GAGATTAAGAAGAAGCAGAA+GGG | + | chr2.4:19233077-19233096 | MS.gene065261:CDS | 35.0% | |
| ! | GTTGGTAGATAAAACTTAGC+TGG | - | chr2.4:19233188-19233207 | None:intergenic | 35.0% |
| CTTCTTAATCTCCATCACTC+CGG | - | chr2.4:19233069-19233088 | None:intergenic | 40.0% | |
| GGAGATTAAGAAGAAGCAGA+AGG | + | chr2.4:19233076-19233095 | MS.gene065261:CDS | 40.0% | |
| ! | ACCAACTGATGATAACGAGA+AGG | + | chr2.4:19233202-19233221 | MS.gene065261:CDS | 40.0% |
| ! | TATTGGTGCTTGTCAAGACT+TGG | + | chr2.4:19233109-19233128 | MS.gene065261:CDS | 40.0% |
| ATGGTTTCAGGGTCTAAGCA+AGG | + | chr2.4:19233226-19233245 | MS.gene065261:CDS | 45.0% | |
| GCCTTCTCGTTATCATCAGT+TGG | - | chr2.4:19233206-19233225 | None:intergenic | 45.0% | |
| TGGTTTCAGGGTCTAAGCAA+GGG | + | chr2.4:19233227-19233246 | MS.gene065261:CDS | 45.0% | |
| ! | AACGAGAAGGCATGGTTTCA+GGG | + | chr2.4:19233215-19233234 | MS.gene065261:CDS | 45.0% |
| ! | AAGCAAGGGTGCAAAAGCTA+AGG | + | chr2.4:19233241-19233260 | MS.gene065261:CDS | 45.0% |
| ! | CTGATGATAACGAGAAGGCA+TGG | + | chr2.4:19233207-19233226 | MS.gene065261:CDS | 45.0% |
| ! | TAACGAGAAGGCATGGTTTC+AGG | + | chr2.4:19233214-19233233 | MS.gene065261:CDS | 45.0% |
| !! | CAGAAGGGTGTTGCAGATAT+TGG | + | chr2.4:19233092-19233111 | MS.gene065261:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 19233056 | 19233328 | 19233056 | ID=MS.gene065261 |
| chr2.4 | mRNA | 19233056 | 19233328 | 19233056 | ID=MS.gene065261.t1;Parent=MS.gene065261 |
| chr2.4 | exon | 19233056 | 19233328 | 19233056 | ID=MS.gene065261.t1.exon1;Parent=MS.gene065261.t1 |
| chr2.4 | CDS | 19233056 | 19233328 | 19233056 | ID=cds.MS.gene065261.t1;Parent=MS.gene065261.t1 |
| Gene Sequence |
| Protein sequence |
