Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065262.t1 | KEH38713.1 | 61 | 187 | 63 | 2 | 5 | 191 | 49 | 225 | 1.60E-40 | 176 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065262.t1 | A0A072VB89 | 61.0 | 187 | 63 | 2 | 5 | 191 | 49 | 225 | 1.2e-40 | 176.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050227 | MS.gene065262 | 0.805692 | 1.18E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene065262 | 0.808812 | 2.58E-50 | -1.69E-46 |
| MS.gene050303 | MS.gene065262 | 0.800116 | 1.68E-48 | -1.69E-46 |
| MS.gene050330 | MS.gene065262 | -0.803929 | 2.76E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene065262 | 0.80055 | 1.37E-48 | -1.69E-46 |
| MS.gene050719 | MS.gene065262 | 0.811269 | 7.62E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene065262 | 0.809581 | 1.76E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene065262 | 0.816297 | 5.95E-52 | -1.69E-46 |
| MS.gene052358 | MS.gene065262 | -0.806088 | 9.75E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene065262 | 0.802406 | 5.70E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene065262 | 0.805396 | 1.36E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene065262 | 0.810738 | 9.93E-51 | -1.69E-46 |
| MS.gene053008 | MS.gene065262 | 0.805432 | 1.34E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene065262 | 0.805537 | 1.27E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene065262 | 0.833654 | 4.71E-56 | -1.69E-46 |
| MS.gene056506 | MS.gene065262 | 0.81964 | 1.04E-52 | -1.69E-46 |
| MS.gene057026 | MS.gene065262 | 0.817388 | 3.38E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene065262 | 0.804319 | 2.29E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene065262 | 0.96083 | 5.29E-119 | -1.69E-46 |
| MS.gene060322 | MS.gene065262 | 0.846687 | 1.86E-59 | -1.69E-46 |
| MS.gene060572 | MS.gene065262 | 0.822527 | 2.26E-53 | -1.69E-46 |
| MS.gene060683 | MS.gene065262 | 0.815583 | 8.58E-52 | -1.69E-46 |
| MS.gene060685 | MS.gene065262 | 0.812881 | 3.39E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene065262 | 0.802062 | 6.71E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene065262 | -0.832832 | 7.55E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065262.t1 | MTR_2g079000 | 79.021 | 143 | 28 | 1 | 49 | 191 | 85 | 225 | 2.24e-68 | 208 |
| MS.gene065262.t1 | MTR_5g012820 | 61.929 | 197 | 48 | 4 | 10 | 179 | 109 | 305 | 2.42e-65 | 204 |
| MS.gene065262.t1 | MTR_5g012820 | 61.929 | 197 | 48 | 4 | 10 | 179 | 119 | 315 | 4.75e-65 | 204 |
| MS.gene065262.t1 | MTR_2g079040 | 87.500 | 40 | 5 | 0 | 133 | 172 | 1 | 40 | 7.42e-16 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065262.t1 | AT5G05210 | 46.078 | 204 | 78 | 6 | 1 | 179 | 144 | 340 | 1.25e-41 | 144 |
| MS.gene065262.t1 | AT5G05210 | 46.078 | 204 | 78 | 6 | 1 | 179 | 136 | 332 | 1.25e-41 | 144 |
| MS.gene065262.t1 | AT2G27750 | 37.356 | 174 | 75 | 6 | 12 | 179 | 3 | 148 | 5.84e-21 | 85.9 |
Find 44 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTGAAGCTATTGCTAAAA+AGG | 0.287342 | 2.4:+19233739 | MS.gene065262:CDS |
| ATACTTCTCTTTATCAGTTT+AGG | 0.301257 | 2.4:-19233815 | None:intergenic |
| TGAAAGAAAGATAGAGTTTC+CGG | 0.308185 | 2.4:+19233553 | MS.gene065262:CDS |
| AGGTCCTAACTGTGCAAATC+TGG | 0.332531 | 2.4:+19233511 | MS.gene065262:CDS |
| GCAGTTAAGCCTTTCATGTC+GGG | 0.372922 | 2.4:+19233410 | MS.gene065262:CDS |
| AGCAGAAAATCGCAGTGAAA+TGG | 0.374864 | 2.4:+19233858 | MS.gene065262:CDS |
| TGAAAGAGCTAAGAAGTTAG+AGG | 0.400614 | 2.4:+19233691 | MS.gene065262:CDS |
| GTATACAGAAATGGAAGAAT+AGG | 0.408150 | 2.4:+19233834 | MS.gene065262:CDS |
| GCAATAGCTTCACCCTTCTC+AGG | 0.419537 | 2.4:-19233731 | None:intergenic |
| AGCAGCAATGAATAGAGCTT+CGG | 0.425070 | 2.4:+19233772 | MS.gene065262:CDS |
| GCAGCAATGAATAGAGCTTC+GGG | 0.426903 | 2.4:+19233773 | MS.gene065262:CDS |
| TCTTTAAAAGAGCTTGTGTT+TGG | 0.437000 | 2.4:+19233605 | MS.gene065262:CDS |
| TCCTTTACCACCTTCTCCTC+CGG | 0.440386 | 2.4:-19233572 | None:intergenic |
| ATCTGGAAAATGCTAATAAG+AGG | 0.444036 | 2.4:+19233528 | MS.gene065262:CDS |
| TAAAGAGAAGTATACAGAAA+TGG | 0.461758 | 2.4:+19233825 | MS.gene065262:CDS |
| GCAGAAAATCGCAGTGAAAT+GGG | 0.472714 | 2.4:+19233859 | MS.gene065262:CDS |
| TCTTTCAAGTTCCTTGTGCT+TGG | 0.476271 | 2.4:-19233675 | None:intergenic |
| GGCAATGTTAAACTTCAGAA+TGG | 0.487362 | 2.4:+19233626 | MS.gene065262:CDS |
| GATAGAGTTTCCGGAGGAGA+AGG | 0.496889 | 2.4:+19233562 | MS.gene065262:CDS |
| GAATAGAGCTTCGGGAATCA+AGG | 0.497557 | 2.4:+19233781 | MS.gene065262:CDS |
| TCCGGAGGAGAAGGTGGTAA+AGG | 0.513098 | 2.4:+19233571 | MS.gene065262:CDS |
| TCAAACAAAGGATCAGTTGA+AGG | 0.525525 | 2.4:+19233899 | MS.gene065262:CDS |
| AAACTTGAAGAGTTTCAGTC+AGG | 0.526291 | 2.4:+19233491 | MS.gene065262:CDS |
| TAAGCCTTTCATGTCGGGGT+TGG | 0.538275 | 2.4:+19233415 | MS.gene065262:CDS |
| TGCAGTTAAGCCTTTCATGT+CGG | 0.538494 | 2.4:+19233409 | MS.gene065262:CDS |
| CCTTTCGCGTAGTTCTTCAA+AGG | 0.546785 | 2.4:-19233459 | None:intergenic |
| CGCAGTGAAATGGGAAGAGA+GGG | 0.547718 | 2.4:+19233868 | MS.gene065262:CDS |
| GCTTCCAACCCCGACATGAA+AGG | 0.556004 | 2.4:-19233419 | None:intergenic |
| CGGGGTTGGAAGCAGATGAT+CGG | 0.561569 | 2.4:+19233429 | MS.gene065262:CDS |
| CTTCAGAATGGTGAAATGCA+AGG | 0.562609 | 2.4:+19233638 | MS.gene065262:CDS |
| TTCAGAATGGTGAAATGCAA+GGG | 0.568164 | 2.4:+19233639 | MS.gene065262:CDS |
| AGAGTTTCCGGAGGAGAAGG+TGG | 0.570130 | 2.4:+19233565 | MS.gene065262:CDS |
| AATGACAGCGATGGAGAGGA+AGG | 0.580649 | 2.4:+19233386 | MS.gene065262:CDS |
| AATGAATGACAGCGATGGAG+AGG | 0.585376 | 2.4:+19233382 | None:intergenic |
| GAAGAAGAATGATCCTGAGA+AGG | 0.589096 | 2.4:+19233718 | MS.gene065262:CDS |
| AAGAAGAATGATCCTGAGAA+GGG | 0.591026 | 2.4:+19233719 | MS.gene065262:CDS |
| CAGTTAAGCCTTTCATGTCG+GGG | 0.594657 | 2.4:+19233411 | MS.gene065262:CDS |
| TCTGGAAAATGCTAATAAGA+GGG | 0.594774 | 2.4:+19233529 | MS.gene065262:CDS |
| TCGCAGTGAAATGGGAAGAG+AGG | 0.597560 | 2.4:+19233867 | MS.gene065262:CDS |
| CCTTTGAAGAACTACGCGAA+AGG | 0.632563 | 2.4:+19233459 | MS.gene065262:CDS |
| AGGGTTCAAAGTTCAAACAA+AGG | 0.644899 | 2.4:+19233887 | MS.gene065262:CDS |
| GAGAAAAGTTTCCAAGCACA+AGG | 0.645702 | 2.4:+19233664 | MS.gene065262:CDS |
| GGAGGAGAAGGTGGTAAAGG+AGG | 0.646856 | 2.4:+19233574 | MS.gene065262:CDS |
| AAGAAAGATAGAGTTTCCGG+AGG | 0.655190 | 2.4:+19233556 | MS.gene065262:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATACTTCTCTTTATCAGTTT+AGG | - | chr2.4:19233818-19233837 | None:intergenic | 25.0% |
| !! | TAAAGAGAAGTATACAGAAA+TGG | + | chr2.4:19233825-19233844 | MS.gene065262:CDS | 25.0% |
| ATCTGGAAAATGCTAATAAG+AGG | + | chr2.4:19233528-19233547 | MS.gene065262:CDS | 30.0% | |
| GTATACAGAAATGGAAGAAT+AGG | + | chr2.4:19233834-19233853 | MS.gene065262:CDS | 30.0% | |
| TCTGGAAAATGCTAATAAGA+GGG | + | chr2.4:19233529-19233548 | MS.gene065262:CDS | 30.0% | |
| TGAAAGAAAGATAGAGTTTC+CGG | + | chr2.4:19233553-19233572 | MS.gene065262:CDS | 30.0% | |
| ! | TCTTTAAAAGAGCTTGTGTT+TGG | + | chr2.4:19233605-19233624 | MS.gene065262:CDS | 30.0% |
| AAACTTGAAGAGTTTCAGTC+AGG | + | chr2.4:19233491-19233510 | MS.gene065262:CDS | 35.0% | |
| AAGAAGAATGATCCTGAGAA+GGG | + | chr2.4:19233719-19233738 | MS.gene065262:CDS | 35.0% | |
| AGGCAGAGAAACAACAAAAA+AGG | + | chr2.4:19233919-19233938 | MS.gene065262:CDS | 35.0% | |
| AGGGTTCAAAGTTCAAACAA+AGG | + | chr2.4:19233887-19233906 | MS.gene065262:CDS | 35.0% | |
| CTATTGCTAAAAAGGAAGCA+TGG | + | chr2.4:19233747-19233766 | MS.gene065262:CDS | 35.0% | |
| GGCAATGTTAAACTTCAGAA+TGG | + | chr2.4:19233626-19233645 | MS.gene065262:CDS | 35.0% | |
| TCAAACAAAGGATCAGTTGA+AGG | + | chr2.4:19233899-19233918 | MS.gene065262:CDS | 35.0% | |
| TGAAAGAGCTAAGAAGTTAG+AGG | + | chr2.4:19233691-19233710 | MS.gene065262:CDS | 35.0% | |
| TTCAGAATGGTGAAATGCAA+GGG | + | chr2.4:19233639-19233658 | MS.gene065262:CDS | 35.0% | |
| ! | TTTTCCAGATTTGCACAGTT+AGG | - | chr2.4:19233518-19233537 | None:intergenic | 35.0% |
| !! | AAGCTCTTTTAAAGACTCTG+TGG | - | chr2.4:19233600-19233619 | None:intergenic | 35.0% |
| AAGAAAGATAGAGTTTCCGG+AGG | + | chr2.4:19233556-19233575 | MS.gene065262:CDS | 40.0% | |
| AGCAGAAAATCGCAGTGAAA+TGG | + | chr2.4:19233858-19233877 | MS.gene065262:CDS | 40.0% | |
| AGCAGCAATGAATAGAGCTT+CGG | + | chr2.4:19233772-19233791 | MS.gene065262:CDS | 40.0% | |
| CTTCAGAATGGTGAAATGCA+AGG | + | chr2.4:19233638-19233657 | MS.gene065262:CDS | 40.0% | |
| GAAGAAGAATGATCCTGAGA+AGG | + | chr2.4:19233718-19233737 | MS.gene065262:CDS | 40.0% | |
| GAGAAAAGTTTCCAAGCACA+AGG | + | chr2.4:19233664-19233683 | MS.gene065262:CDS | 40.0% | |
| GCAGAAAATCGCAGTGAAAT+GGG | + | chr2.4:19233859-19233878 | MS.gene065262:CDS | 40.0% | |
| GGGTGAAGCTATTGCTAAAA+AGG | + | chr2.4:19233739-19233758 | MS.gene065262:CDS | 40.0% | |
| TGCAGTTAAGCCTTTCATGT+CGG | + | chr2.4:19233409-19233428 | MS.gene065262:CDS | 40.0% | |
| !! | TCTTTCAAGTTCCTTGTGCT+TGG | - | chr2.4:19233678-19233697 | None:intergenic | 40.0% |
| AGGTCCTAACTGTGCAAATC+TGG | + | chr2.4:19233511-19233530 | MS.gene065262:CDS | 45.0% | |
| CAGTTAAGCCTTTCATGTCG+GGG | + | chr2.4:19233411-19233430 | MS.gene065262:CDS | 45.0% | |
| CCTTTCGCGTAGTTCTTCAA+AGG | - | chr2.4:19233462-19233481 | None:intergenic | 45.0% | |
| GAATAGAGCTTCGGGAATCA+AGG | + | chr2.4:19233781-19233800 | MS.gene065262:CDS | 45.0% | |
| GCAGCAATGAATAGAGCTTC+GGG | + | chr2.4:19233773-19233792 | MS.gene065262:CDS | 45.0% | |
| GCAGTTAAGCCTTTCATGTC+GGG | + | chr2.4:19233410-19233429 | MS.gene065262:CDS | 45.0% | |
| ! | CCTTTGAAGAACTACGCGAA+AGG | + | chr2.4:19233459-19233478 | MS.gene065262:CDS | 45.0% |
| AATGACAGCGATGGAGAGGA+AGG | + | chr2.4:19233386-19233405 | MS.gene065262:CDS | 50.0% | |
| CGCAGTGAAATGGGAAGAGA+GGG | + | chr2.4:19233868-19233887 | MS.gene065262:CDS | 50.0% | |
| GATAGAGTTTCCGGAGGAGA+AGG | + | chr2.4:19233562-19233581 | MS.gene065262:CDS | 50.0% | |
| GCAATAGCTTCACCCTTCTC+AGG | - | chr2.4:19233734-19233753 | None:intergenic | 50.0% | |
| TAAGCCTTTCATGTCGGGGT+TGG | + | chr2.4:19233415-19233434 | MS.gene065262:CDS | 50.0% | |
| TCCTTTACCACCTTCTCCTC+CGG | - | chr2.4:19233575-19233594 | None:intergenic | 50.0% | |
| TCGCAGTGAAATGGGAAGAG+AGG | + | chr2.4:19233867-19233886 | MS.gene065262:CDS | 50.0% | |
| AGAGTTTCCGGAGGAGAAGG+TGG | + | chr2.4:19233565-19233584 | MS.gene065262:CDS | 55.0% | |
| CGGGGTTGGAAGCAGATGAT+CGG | + | chr2.4:19233429-19233448 | MS.gene065262:CDS | 55.0% | |
| GCTTCCAACCCCGACATGAA+AGG | - | chr2.4:19233422-19233441 | None:intergenic | 55.0% | |
| GGAGGAGAAGGTGGTAAAGG+AGG | + | chr2.4:19233574-19233593 | MS.gene065262:CDS | 55.0% | |
| TCCGGAGGAGAAGGTGGTAA+AGG | + | chr2.4:19233571-19233590 | MS.gene065262:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 19233383 | 19233958 | 19233383 | ID=MS.gene065262 |
| chr2.4 | mRNA | 19233383 | 19233958 | 19233383 | ID=MS.gene065262.t1;Parent=MS.gene065262 |
| chr2.4 | exon | 19233383 | 19233958 | 19233383 | ID=MS.gene065262.t1.exon1;Parent=MS.gene065262.t1 |
| chr2.4 | CDS | 19233383 | 19233958 | 19233383 | ID=cds.MS.gene065262.t1;Parent=MS.gene065262.t1 |
| Gene Sequence |
| Protein sequence |
