Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065762.t1 | RHN52760.1 | 93.7 | 142 | 8 | 1 | 1 | 141 | 1 | 142 | 2.20E-69 | 271.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065762.t1 | P09761 | 54.1 | 122 | 51 | 2 | 25 | 143 | 80 | 199 | 1.3e-35 | 150.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065762.t1 | A0A072UMD3 | 93.7 | 142 | 8 | 1 | 1 | 141 | 1 | 142 | 1.6e-69 | 271.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051525 | MS.gene065762 | 0.838661 | 2.52E-57 | -1.69E-46 |
| MS.gene051582 | MS.gene065762 | 0.821107 | 4.81E-53 | -1.69E-46 |
| MS.gene052253 | MS.gene065762 | 0.803542 | 3.32E-49 | -1.69E-46 |
| MS.gene052501 | MS.gene065762 | 0.81635 | 5.79E-52 | -1.69E-46 |
| MS.gene052505 | MS.gene065762 | 0.819104 | 1.38E-52 | -1.69E-46 |
| MS.gene052982 | MS.gene065762 | 0.828027 | 1.13E-54 | -1.69E-46 |
| MS.gene053330 | MS.gene065762 | 0.813453 | 2.54E-51 | -1.69E-46 |
| MS.gene053873 | MS.gene065762 | 0.806047 | 9.94E-50 | -1.69E-46 |
| MS.gene054102 | MS.gene065762 | 0.867817 | 1.03E-65 | -1.69E-46 |
| MS.gene054110 | MS.gene065762 | 0.840367 | 9.08E-58 | -1.69E-46 |
| MS.gene05412 | MS.gene065762 | 0.85081 | 1.33E-60 | -1.69E-46 |
| MS.gene05413 | MS.gene065762 | 0.935301 | 1.05E-96 | -1.69E-46 |
| MS.gene054719 | MS.gene065762 | 0.80637 | 8.50E-50 | -1.69E-46 |
| MS.gene054918 | MS.gene065762 | 0.81846 | 1.94E-52 | -1.69E-46 |
| MS.gene054920 | MS.gene065762 | 0.816402 | 5.63E-52 | -1.69E-46 |
| MS.gene055369 | MS.gene065762 | 0.806362 | 8.53E-50 | -1.69E-46 |
| MS.gene056497 | MS.gene065762 | 0.816022 | 6.85E-52 | -1.69E-46 |
| MS.gene056514 | MS.gene065762 | 0.84997 | 2.30E-60 | -1.69E-46 |
| MS.gene057048 | MS.gene065762 | 0.803143 | 4.01E-49 | -1.69E-46 |
| MS.gene057825 | MS.gene065762 | 0.818068 | 2.38E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene065762 | 0.800715 | 1.27E-48 | -1.69E-46 |
| MS.gene058444 | MS.gene065762 | 0.81539 | 9.47E-52 | -1.69E-46 |
| MS.gene059420 | MS.gene065762 | 0.806013 | 1.01E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene065762 | 0.802548 | 5.33E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065762.t1 | MTR_6g083200 | 93.662 | 142 | 8 | 1 | 1 | 141 | 1 | 142 | 7.05e-94 | 270 |
| MS.gene065762.t1 | MTR_6g083270 | 65.839 | 161 | 37 | 3 | 1 | 143 | 1 | 161 | 3.72e-67 | 210 |
| MS.gene065762.t1 | MTR_6g083310 | 63.462 | 156 | 43 | 3 | 1 | 142 | 1 | 156 | 8.07e-64 | 192 |
| MS.gene065762.t1 | MTR_6g083290 | 63.333 | 150 | 43 | 4 | 1 | 142 | 1 | 146 | 1.24e-57 | 182 |
| MS.gene065762.t1 | MTR_1g080800 | 52.143 | 140 | 59 | 3 | 10 | 143 | 9 | 146 | 1.07e-48 | 153 |
| MS.gene065762.t1 | MTR_1g080780 | 52.985 | 134 | 57 | 3 | 13 | 143 | 11 | 141 | 2.16e-48 | 152 |
| MS.gene065762.t1 | MTR_1g080820 | 51.449 | 138 | 59 | 3 | 10 | 141 | 9 | 144 | 1.25e-46 | 148 |
| MS.gene065762.t1 | MTR_1g080810 | 48.905 | 137 | 62 | 3 | 11 | 141 | 10 | 144 | 2.22e-44 | 142 |
| MS.gene065762.t1 | MTR_7g115220 | 52.101 | 119 | 52 | 2 | 27 | 142 | 69 | 185 | 2.17e-40 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065762.t1 | AT3G04720 | 48.780 | 123 | 58 | 2 | 23 | 142 | 72 | 192 | 3.83e-39 | 131 |
Find 32 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAATCCTCATAAAATTAAA+TGG | 0.257556 | 6.2:-82100405 | MS.gene065762:CDS |
| TTGTCATGGCGTAGTGAATT+TGG | 0.312460 | 6.2:-82100331 | MS.gene065762:CDS |
| CAGGTGACAAACAAAGAAAA+TGG | 0.324037 | 6.2:-82099424 | MS.gene065762:CDS |
| AAGGTAACTTTGAGCATTCC+CGG | 0.379943 | 6.2:+82099303 | None:intergenic |
| GAATGCTCAAAGTTACCTTA+TGG | 0.411312 | 6.2:-82099299 | MS.gene065762:CDS |
| CCACAAACACCTTTGCCAAC+AGG | 0.421503 | 6.2:+82100289 | None:intergenic |
| AAATGGAACTTGAACACATC+TGG | 0.431997 | 6.2:-82100388 | MS.gene065762:CDS |
| TATTGATGTGTTTCAAAAGC+TGG | 0.434145 | 6.2:-82099338 | MS.gene065762:CDS |
| TTTCAAAAGCTGGATAGCTC+TGG | 0.449489 | 6.2:-82099328 | MS.gene065762:CDS |
| GATTATGAATTTGTGGATTG+TGG | 0.458700 | 6.2:-82099274 | MS.gene065762:CDS |
| TATGGTGGATTATGAATTTG+TGG | 0.478849 | 6.2:-82099281 | MS.gene065762:CDS |
| TGGTGTGTTCTGTGCAGCTC+AGG | 0.478988 | 6.2:-82100368 | MS.gene065762:CDS |
| TTCTGTGGACCTGTTGGCAA+AGG | 0.480910 | 6.2:-82100298 | MS.gene065762:CDS |
| CAAATTCATAATCCACCATA+AGG | 0.490614 | 6.2:+82099284 | None:intergenic |
| TTTGGCTGGACTGCCTTCTG+TGG | 0.508213 | 6.2:-82100313 | MS.gene065762:CDS |
| AGGTGACAAACAAAGAAAAT+GGG | 0.510288 | 6.2:-82099423 | MS.gene065762:CDS |
| CCTGTTGGCAAAGGTGTTTG+TGG | 0.529107 | 6.2:-82100289 | MS.gene065762:CDS |
| TGTTCTGTGCAGCTCAGGAT+GGG | 0.532727 | 6.2:-82100363 | MS.gene065762:CDS |
| AGCTGGATAGCTCTGGGACC+GGG | 0.539373 | 6.2:-82099321 | MS.gene065762:CDS |
| CATGGCGTAGTGAATTTGGC+TGG | 0.539611 | 6.2:-82100327 | MS.gene065762:CDS |
| ACTGCCTTCTGTGGACCTGT+TGG | 0.548588 | 6.2:-82100304 | MS.gene065762:CDS |
| AAGCTGGATAGCTCTGGGAC+CGG | 0.549261 | 6.2:-82099322 | MS.gene065762:CDS |
| GTGTTCTGTGCAGCTCAGGA+TGG | 0.554622 | 6.2:-82100364 | MS.gene065762:CDS |
| ATCTAACTCAAGCCCTCCGT+TGG | 0.561496 | 6.2:+82099360 | None:intergenic |
| TTCAAAAGCTGGATAGCTCT+GGG | 0.561619 | 6.2:-82099327 | MS.gene065762:CDS |
| TGCTCAAAGTTACCTTATGG+TGG | 0.566436 | 6.2:-82099296 | MS.gene065762:CDS |
| TTGATACATGCACCAACGGA+GGG | 0.588242 | 6.2:-82099372 | MS.gene065762:CDS |
| ATGGGAACAAATCATTGTCA+TGG | 0.598375 | 6.2:-82100345 | MS.gene065762:CDS |
| TTTGCCAACAGGTCCACAGA+AGG | 0.647196 | 6.2:+82100300 | None:intergenic |
| TCAAAGAGCTAACAACGTGA+AGG | 0.649689 | 6.2:-82100443 | MS.gene065762:CDS |
| GTTGATACATGCACCAACGG+AGG | 0.651216 | 6.2:-82099373 | MS.gene065762:CDS |
| ATTGTTGATACATGCACCAA+CGG | 0.654994 | 6.2:-82099376 | MS.gene065762:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAGTTATATATTTGTTT+GGG | - | chr6.2:82100164-82100183 | MS.gene065762:intron | 10.0% |
| !! | AATTTCAATATAAACAAATT+TGG | + | chr6.2:82099966-82099985 | None:intergenic | 10.0% |
| !! | AATTTACTTAAGATTTAAGA+CGG | + | chr6.2:82099634-82099653 | None:intergenic | 15.0% |
| !! | CAAAAAGTTATATATTTGTT+TGG | - | chr6.2:82100163-82100182 | MS.gene065762:intron | 15.0% |
| !! | TATTGTTTACTTCATATATA+TGG | - | chr6.2:82100265-82100284 | MS.gene065762:intron | 15.0% |
| !! | TTATTAATCAATAAAGTTCT+AGG | + | chr6.2:82100301-82100320 | None:intergenic | 15.0% |
| !!! | AAAAAGCATTTTACATATTA+CGG | - | chr6.2:82100027-82100046 | MS.gene065762:intron | 15.0% |
| !!! | GCTTAAGTATTTTTTTTTTA+AGG | + | chr6.2:82099667-82099686 | None:intergenic | 15.0% |
| !! | AATAACTAACACTATTGAAT+TGG | - | chr6.2:82099837-82099856 | MS.gene065762:intron | 20.0% |
| !! | ACAATCCTCATAAAATTAAA+TGG | - | chr6.2:82099370-82099389 | MS.gene065762:CDS | 20.0% |
| !! | TATATTTACAACATCAAACT+TGG | + | chr6.2:82099891-82099910 | None:intergenic | 20.0% |
| !! | TCCTAAATTCCTAATAAATA+GGG | + | chr6.2:82099818-82099837 | None:intergenic | 20.0% |
| !! | TTCCTAAATTCCTAATAAAT+AGG | + | chr6.2:82099819-82099838 | None:intergenic | 20.0% |
| !!! | AAGTTCCATTTAATTTTATG+AGG | + | chr6.2:82099378-82099397 | None:intergenic | 20.0% |
| ! | CAATATAAACAAATTTGGCA+AGG | + | chr6.2:82099961-82099980 | None:intergenic | 25.0% |
| ! | CCTAAATTCCTAATAAATAG+GGG | + | chr6.2:82099817-82099836 | None:intergenic | 25.0% |
| ! | CTATTGAATTGGATACTATA+AGG | - | chr6.2:82099848-82099867 | MS.gene065762:intron | 25.0% |
| ! | TGAGAGAATAAAACGATAAT+TGG | + | chr6.2:82099919-82099938 | None:intergenic | 25.0% |
| AGGTGACAAACAAAGAAAAT+GGG | - | chr6.2:82100352-82100371 | MS.gene065762:CDS | 30.0% | |
| ATTCCTAATAAATAGGGGAT+CGG | + | chr6.2:82099812-82099831 | None:intergenic | 30.0% | |
| CAAATTCATAATCCACCATA+AGG | + | chr6.2:82100494-82100513 | None:intergenic | 30.0% | |
| CAACATCAAACTTGGTAAAT+TGG | + | chr6.2:82099883-82099902 | None:intergenic | 30.0% | |
| CCCCTATTTATTAGGAATTT+AGG | - | chr6.2:82099814-82099833 | MS.gene065762:intron | 30.0% | |
| TATTGATGTGTTTCAAAAGC+TGG | - | chr6.2:82100437-82100456 | MS.gene065762:CDS | 30.0% | |
| ! | AGCTGGTCTAATTAGAAAAT+TGG | - | chr6.2:82099576-82099595 | MS.gene065762:intron | 30.0% |
| ! | GATTATGAATTTGTGGATTG+TGG | - | chr6.2:82100501-82100520 | MS.gene065762:CDS | 30.0% |
| !! | ATCGTCTAATTTTGATCAAC+CGG | - | chr6.2:82099730-82099749 | MS.gene065762:intron | 30.0% |
| !! | TATGGTGGATTATGAATTTG+TGG | - | chr6.2:82100494-82100513 | MS.gene065762:CDS | 30.0% |
| AAATGGAACTTGAACACATC+TGG | - | chr6.2:82099387-82099406 | MS.gene065762:CDS | 35.0% | |
| ATGGGAACAAATCATTGTCA+TGG | - | chr6.2:82099430-82099449 | MS.gene065762:intron | 35.0% | |
| ATTGTTGATACATGCACCAA+CGG | - | chr6.2:82100399-82100418 | MS.gene065762:CDS | 35.0% | |
| CAGGTGACAAACAAAGAAAA+TGG | - | chr6.2:82100351-82100370 | MS.gene065762:CDS | 35.0% | |
| GAATGCTCAAAGTTACCTTA+TGG | - | chr6.2:82100476-82100495 | MS.gene065762:CDS | 35.0% | |
| GAGCAATCAACAATGACAAT+GGG | - | chr6.2:82099780-82099799 | MS.gene065762:intron | 35.0% | |
| TCTATTGTGCTTAAATCAGC+TGG | - | chr6.2:82099559-82099578 | MS.gene065762:intron | 35.0% | |
| TGAGCAATCAACAATGACAA+TGG | - | chr6.2:82099779-82099798 | MS.gene065762:intron | 35.0% | |
| TGAGCTAAAACAAGACAAGA+GGG | - | chr6.2:82100079-82100098 | MS.gene065762:intron | 35.0% | |
| TTCCTAATAAATAGGGGATC+GGG | + | chr6.2:82099811-82099830 | None:intergenic | 35.0% | |
| !! | ATTAGTCATGTACTGATTGC+TGG | - | chr6.2:82100321-82100340 | MS.gene065762:CDS | 35.0% |
| !! | GCAGCTTTTTCTATTGGATA+AGG | - | chr6.2:82099275-82099294 | MS.gene065762:CDS | 35.0% |
| AAGGTAACTTTGAGCATTCC+CGG | + | chr6.2:82100475-82100494 | None:intergenic | 40.0% | |
| ATCCCGATCCCCTATTTATT+AGG | - | chr6.2:82099806-82099825 | MS.gene065762:intron | 40.0% | |
| GTGAGCTAAAACAAGACAAG+AGG | - | chr6.2:82100078-82100097 | MS.gene065762:intron | 40.0% | |
| TCAAAGAGCTAACAACGTGA+AGG | - | chr6.2:82099332-82099351 | MS.gene065762:CDS | 40.0% | |
| TGCTCAAAGTTACCTTATGG+TGG | - | chr6.2:82100479-82100498 | MS.gene065762:CDS | 40.0% | |
| TTCAAAAGCTGGATAGCTCT+GGG | - | chr6.2:82100448-82100467 | MS.gene065762:CDS | 40.0% | |
| TTGTCATGGCGTAGTGAATT+TGG | - | chr6.2:82099444-82099463 | MS.gene065762:intron | 40.0% | |
| TTTCAAAAGCTGGATAGCTC+TGG | - | chr6.2:82100447-82100466 | MS.gene065762:CDS | 40.0% | |
| !! | GTCGAAGCAGCTTTTTCTAT+TGG | - | chr6.2:82099269-82099288 | MS.gene065762:CDS | 40.0% |
| TTGATACATGCACCAACGGA+GGG | - | chr6.2:82100403-82100422 | MS.gene065762:CDS | 45.0% | |
| !!! | GTATTATTTTTTTTTTTTTA+AGG | + | chr6.2:82099693-82099712 | None:intergenic | 5.0% |
| !!! | TTAATATTTATTTTATTCTT+AGG | - | chr6.2:82099993-82100012 | MS.gene065762:intron | 5.0% |
| ACTGATTGCTGGTCGATTGC+AGG | - | chr6.2:82100332-82100351 | MS.gene065762:CDS | 50.0% | |
| ATCTAACTCAAGCCCTCCGT+TGG | + | chr6.2:82100418-82100437 | None:intergenic | 50.0% | |
| CATGGCGTAGTGAATTTGGC+TGG | - | chr6.2:82099448-82099467 | MS.gene065762:intron | 50.0% | |
| CCACAAACACCTTTGCCAAC+AGG | + | chr6.2:82099489-82099508 | None:intergenic | 50.0% | |
| GTTGATACATGCACCAACGG+AGG | - | chr6.2:82100402-82100421 | MS.gene065762:CDS | 50.0% | |
| GTTGTTTGTGACTGCGTGAC+CGG | + | chr6.2:82099752-82099771 | None:intergenic | 50.0% | |
| TGTTCTGTGCAGCTCAGGAT+GGG | - | chr6.2:82099412-82099431 | MS.gene065762:CDS | 50.0% | |
| TTCTGTGGACCTGTTGGCAA+AGG | - | chr6.2:82099477-82099496 | MS.gene065762:intron | 50.0% | |
| TTTGCCAACAGGTCCACAGA+AGG | + | chr6.2:82099478-82099497 | None:intergenic | 50.0% | |
| !! | CCTGTTGGCAAAGGTGTTTG+TGG | - | chr6.2:82099486-82099505 | MS.gene065762:intron | 50.0% |
| ACTGCCTTCTGTGGACCTGT+TGG | - | chr6.2:82099471-82099490 | MS.gene065762:intron | 55.0% | |
| GTGTTCTGTGCAGCTCAGGA+TGG | - | chr6.2:82099411-82099430 | MS.gene065762:CDS | 55.0% | |
| TGGTGTGTTCTGTGCAGCTC+AGG | - | chr6.2:82099407-82099426 | MS.gene065762:CDS | 55.0% | |
| TTTGGCTGGACTGCCTTCTG+TGG | - | chr6.2:82099462-82099481 | MS.gene065762:intron | 55.0% | |
| !! | AAGCTGGATAGCTCTGGGAC+CGG | - | chr6.2:82100453-82100472 | MS.gene065762:CDS | 55.0% |
| !! | AGCTGGATAGCTCTGGGACC+GGG | - | chr6.2:82100454-82100473 | MS.gene065762:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 82099267 | 82100530 | 82099267 | ID=MS.gene065762 |
| chr6.2 | mRNA | 82099267 | 82100530 | 82099267 | ID=MS.gene065762.t1;Parent=MS.gene065762 |
| chr6.2 | exon | 82100276 | 82100530 | 82100276 | ID=MS.gene065762.t1.exon1;Parent=MS.gene065762.t1 |
| chr6.2 | CDS | 82100276 | 82100530 | 82100276 | ID=cds.MS.gene065762.t1;Parent=MS.gene065762.t1 |
| chr6.2 | exon | 82099267 | 82099443 | 82099267 | ID=MS.gene065762.t1.exon2;Parent=MS.gene065762.t1 |
| chr6.2 | CDS | 82099267 | 82099443 | 82099267 | ID=cds.MS.gene065762.t1;Parent=MS.gene065762.t1 |
| Gene Sequence |
| Protein sequence |
