AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057825


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057825.t1 MTR_4g076940 98.824 85 1 0 1 85 360 444 4.41e-52 171
MS.gene057825.t1 MTR_4g076940 98.824 85 1 0 1 85 453 537 1.18e-51 171
MS.gene057825.t1 MTR_4g076940 98.824 85 1 0 1 85 493 577 2.15e-51 171
MS.gene057825.t1 MTR_4g076900 87.059 85 11 0 1 85 429 513 4.24e-47 158
MS.gene057825.t1 MTR_4g076970 58.824 85 35 0 1 85 451 535 1.46e-28 107
MS.gene057825.t1 MTR_6g066240 55.263 76 34 0 1 76 464 539 1.05e-22 90.5
MS.gene057825.t1 MTR_4g094050 44.578 83 46 0 2 84 465 547 1.85e-21 87.0
MS.gene057825.t1 MTR_4g094010 47.619 84 44 0 1 84 443 526 2.11e-21 86.7
MS.gene057825.t1 MTR_4g094060 45.238 84 46 0 1 84 464 547 4.77e-20 82.8
MS.gene057825.t1 MTR_4g054020 44.048 84 47 0 1 84 362 445 4.33e-18 77.4
MS.gene057825.t1 MTR_4g094090 40.476 84 50 0 1 84 420 503 3.32e-16 72.0
MS.gene057825.t1 MTR_4g093845 34.524 84 55 0 1 84 450 533 4.67e-15 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057825.t1 AT3G21090 71.765 85 24 0 1 85 445 529 1.19e-36 130
MS.gene057825.t1 AT1G51500 71.053 76 22 0 1 76 446 521 6.50e-31 114
MS.gene057825.t1 AT1G17840 55.263 76 34 0 1 76 467 542 3.87e-24 94.7
MS.gene057825.t1 AT1G51460 44.706 85 47 0 1 85 440 524 9.59e-20 82.0

Find 12 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATTCCCATGAGAAAATT+TGG 0.154614 4.4:+36285933 None:intergenic
TCTTTCATCATTCCCATTCT+TGG 0.167734 4.4:-36286102 MS.gene057825:CDS
TTTCTCATGGGAATCATTAC+AGG 0.293620 4.4:-36285927 MS.gene057825:CDS
CATATAACATGGTGAAATTC+AGG 0.319419 4.4:-36286049 MS.gene057825:CDS
GAAAGGATGAATGGATATTA+TGG 0.342617 4.4:-36286152 None:intergenic
AGAGCAATAGCAACCAAGAA+TGG 0.439146 4.4:+36286089 None:intergenic
ATGGGAATCATTACAGGAGC+TGG 0.460308 4.4:-36285921 MS.gene057825:CDS
GAGCAATAGCAACCAAGAAT+GGG 0.509341 4.4:+36286090 None:intergenic
AACGCATAGTGAATGAATCC+TGG 0.547925 4.4:+36286026 None:intergenic
AACATGGTGAAATTCAGGCC+AGG 0.575455 4.4:-36286044 MS.gene057825:CDS
AGTGATTGAGAGTCTCATGA+TGG 0.667256 4.4:-36285973 MS.gene057825:CDS
TTGCACAATCACATATAACA+TGG 0.677547 4.4:-36286060 MS.gene057825:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTTCACTGTTAACATCTA+TGG - chr4.4:36286055-36286074 MS.gene057825:CDS 25.0%
ATGATTCCCATGAGAAAATT+TGG + chr4.4:36286130-36286149 None:intergenic 30.0%
CATATAACATGGTGAAATTC+AGG - chr4.4:36286011-36286030 MS.gene057825:CDS 30.0%
TTGCACAATCACATATAACA+TGG - chr4.4:36286000-36286019 MS.gene057825:CDS 30.0%
! ACTTGTTCCAAATTTTCTCA+TGG - chr4.4:36286120-36286139 MS.gene057825:CDS 30.0%
! CTTGTTCCAAATTTTCTCAT+GGG - chr4.4:36286121-36286140 MS.gene057825:CDS 30.0%
TCTTTCATCATTCCCATTCT+TGG - chr4.4:36285958-36285977 MS.gene057825:CDS 35.0%
TTTCTCATGGGAATCATTAC+AGG - chr4.4:36286133-36286152 MS.gene057825:CDS 35.0%
AACGCATAGTGAATGAATCC+TGG + chr4.4:36286037-36286056 None:intergenic 40.0%
AGAGCAATAGCAACCAAGAA+TGG + chr4.4:36285974-36285993 None:intergenic 40.0%
AGTGATTGAGAGTCTCATGA+TGG - chr4.4:36286087-36286106 MS.gene057825:CDS 40.0%
GAGCAATAGCAACCAAGAAT+GGG + chr4.4:36285973-36285992 None:intergenic 40.0%
AACATGGTGAAATTCAGGCC+AGG - chr4.4:36286016-36286035 MS.gene057825:CDS 45.0%
ATGGGAATCATTACAGGAGC+TGG - chr4.4:36286139-36286158 MS.gene057825:CDS 45.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 36285914 36286168 36285914 ID=MS.gene057825
chr4.4 mRNA 36285914 36286168 36285914 ID=MS.gene057825.t1;Parent=MS.gene057825
chr4.4 exon 36285914 36286168 36285914 ID=MS.gene057825.t1.exon1;Parent=MS.gene057825.t1
chr4.4 CDS 36285914 36286168 36285914 ID=cds.MS.gene057825.t1;Parent=MS.gene057825.t1
Gene Sequence

>MS.gene057825

ATGAATGGATATTATGGTGTAGCAGCATTTATTTTATCAAACTTTCTTTCATCATTCCCATTCTTGGTTGCTATTGCTCTTACTTCTTGCACAATCACATATAACATGGTGAAATTCAGGCCAGGATTCATTCACTATGCGTTTTTCACTGTTAACATCTATGGATGCATCTCAGTGATTGAGAGTCTCATGATGGTTGTAGCTGCACTTGTTCCAAATTTTCTCATGGGAATCATTACAGGAGCTGGAATCATT

Protein sequence

>MS.gene057825.t1

MNGYYGVAAFILSNFLSSFPFLVAIALTSCTITYNMVKFRPGFIHYAFFTVNIYGCISVIESLMMVVAALVPNFLMGIITGAGII