AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054918


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054918.t1 MTR_1g061630 92.500 80 6 0 79 158 148 227 1.88e-44 152
MS.gene054918.t1 MTR_1g061630 92.500 80 6 0 79 158 164 243 2.13e-44 153
MS.gene054918.t1 MTR_7g103420 80.000 80 16 0 79 158 75 154 2.69e-39 138
MS.gene054918.t1 MTR_7g103420 80.000 80 16 0 79 158 164 243 8.42e-39 138
MS.gene054918.t1 MTR_7g103420 80.000 80 16 0 79 158 164 243 9.93e-39 138
MS.gene054918.t1 MTR_1g091040 70.000 80 23 1 79 158 164 242 1.30e-31 118
MS.gene054918.t1 MTR_1g091040 67.089 79 25 1 80 158 32 109 1.31e-29 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054918.t1 AT3G52990 70.886 79 23 0 79 157 111 189 2.64e-34 125
MS.gene054918.t1 AT3G52990 70.886 79 23 0 79 157 164 242 4.11e-34 125
MS.gene054918.t1 AT2G36580 70.000 80 24 0 79 158 164 243 4.45e-34 125

Find 37 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGATTTACCTACCCTTA+TGG 0.248767 7.3:+63554287 MS.gene054918:CDS
TCGTGCCAAGGCCAAGTTAA+TGG 0.289557 7.3:+63554146 MS.gene054918:CDS
CTAGAAACGGATTGATGATA+TGG 0.306700 7.3:-63553997 None:intergenic
CTCCTTGTCTTTCTCCATAA+GGG 0.360726 7.3:-63554301 None:intergenic
TTCAACATCCTTCTCTAATT+CGG 0.366468 7.3:-63554100 None:intergenic
CGATGGATCATTATATCCTT+CGG 0.374463 7.3:+63554029 MS.gene054918:CDS
GGCTCGAGACTTCCTTTCTT+AGG 0.375163 7.3:+63554404 MS.gene054918:CDS
CCTCCTTGTCTTTCTCCATA+AGG 0.387352 7.3:-63554302 None:intergenic
TATCCTTCGGGAATGGAATC+AGG 0.401428 7.3:+63554042 MS.gene054918:CDS
TTCTGTCATTGTCATATACT+AGG 0.404351 7.3:+63554361 MS.gene054918:CDS
GATGGATCATTATATCCTTC+GGG 0.434441 7.3:+63554030 MS.gene054918:CDS
GACAAGGAGGTTATAAGCAC+CGG 0.445526 7.3:+63554315 MS.gene054918:CDS
GTTTCTAGTTTCTACATCGA+TGG 0.479901 7.3:+63554012 MS.gene054918:CDS
AACTATGATTCGCCTAAGAA+AGG 0.485109 7.3:-63554416 None:intergenic
AAGAATTCAGCTATATTGAC+CGG 0.495338 7.3:+63554231 MS.gene054918:CDS
ATCATTATATCCTTCGGGAA+TGG 0.527875 7.3:+63554035 MS.gene054918:CDS
CCATAAGGGTAGGTAAATCA+AGG 0.534935 7.3:-63554287 None:intergenic
ACTTTAGATACTTCAAGCCA+TGG 0.549646 7.3:-63554180 None:intergenic
ACAAGGAGGTTATAAGCACC+GGG 0.561801 7.3:+63554316 MS.gene054918:CDS
CTTGAAGTATCTAAAGTCAA+AGG 0.563959 7.3:+63554186 MS.gene054918:CDS
ATCGATGTAGAAACTAGAAA+CGG 0.569695 7.3:-63554010 None:intergenic
TTGTCTTTCTCCATAAGGGT+AGG 0.580232 7.3:-63554297 None:intergenic
AGGAGATGAACTTCGTGCCA+AGG 0.591262 7.3:+63554134 MS.gene054918:CDS
TAAATCAAGGTGAATTTGAG+AGG 0.594745 7.3:-63554274 None:intergenic
CATCGATGCCGAATTAGAGA+AGG 0.606419 7.3:+63554092 MS.gene054918:CDS
TAATGGAAAAGAAATTGCCA+TGG 0.625221 7.3:+63554163 MS.gene054918:CDS
ATGCAAAGTGAACAATGACC+CGG 0.628706 7.3:-63554250 None:intergenic
CTTCCTGATTCCATTCCCGA+AGG 0.630450 7.3:-63554045 None:intergenic
GAATGGAATCAGGAAGCTCG+TGG 0.656961 7.3:+63554052 MS.gene054918:CDS
AGAATTCAGCTATATTGACC+GGG 0.657922 7.3:+63554232 MS.gene054918:CDS
AAGGAGGTTATAAGCACCGG+GGG 0.682904 7.3:+63554318 MS.gene054918:CDS
TACCCTTATGGAGAAAGACA+AGG 0.688386 7.3:+63554299 MS.gene054918:CDS
GATGTTGAACGTGTTCATCG+AGG 0.713960 7.3:+63554114 MS.gene054918:CDS
AAAGGAAGTCTCGAGCCTCG+TGG 0.715046 7.3:-63554398 None:intergenic
CCTTATGGAGAAAGACAAGG+AGG 0.717122 7.3:+63554302 MS.gene054918:CDS
CAAGGAGGTTATAAGCACCG+GGG 0.754336 7.3:+63554317 MS.gene054918:CDS
GCATGCTAAAGATGTCCACG+AGG 0.772571 7.3:+63554383 MS.gene054918:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATAAAAAATAATAATTTG+TGG + chr7.3:63553959-63553978 MS.gene054918:CDS 10.0%
!! CAATTTCTTTTCCATTAACT+TGG - chr7.3:63554160-63554179 None:intergenic 25.0%
AAGAATTCAGCTATATTGAC+CGG + chr7.3:63554231-63554250 MS.gene054918:CDS 30.0%
ATCGATGTAGAAACTAGAAA+CGG - chr7.3:63554013-63554032 None:intergenic 30.0%
TAAATCAAGGTGAATTTGAG+AGG - chr7.3:63554277-63554296 None:intergenic 30.0%
TAATGGAAAAGAAATTGCCA+TGG + chr7.3:63554163-63554182 MS.gene054918:CDS 30.0%
TTCAACATCCTTCTCTAATT+CGG - chr7.3:63554103-63554122 None:intergenic 30.0%
TTCTGTCATTGTCATATACT+AGG + chr7.3:63554361-63554380 MS.gene054918:CDS 30.0%
! CTTGAAGTATCTAAAGTCAA+AGG + chr7.3:63554186-63554205 MS.gene054918:CDS 30.0%
!!! AATTTTGTTTTTAACTCCCC+CGG - chr7.3:63554337-63554356 None:intergenic 30.0%
ACTTTAGATACTTCAAGCCA+TGG - chr7.3:63554183-63554202 None:intergenic 35.0%
AGAATTCAGCTATATTGACC+GGG + chr7.3:63554232-63554251 MS.gene054918:CDS 35.0%
ATCATTATATCCTTCGGGAA+TGG + chr7.3:63554035-63554054 MS.gene054918:CDS 35.0%
CGATGGATCATTATATCCTT+CGG + chr7.3:63554029-63554048 MS.gene054918:CDS 35.0%
GATGGATCATTATATCCTTC+GGG + chr7.3:63554030-63554049 MS.gene054918:CDS 35.0%
!! CTAGAAACGGATTGATGATA+TGG - chr7.3:63554000-63554019 None:intergenic 35.0%
!! GTTTCTAGTTTCTACATCGA+TGG + chr7.3:63554012-63554031 MS.gene054918:CDS 35.0%
ATGCAAAGTGAACAATGACC+CGG - chr7.3:63554253-63554272 None:intergenic 40.0%
CCATAAGGGTAGGTAAATCA+AGG - chr7.3:63554290-63554309 None:intergenic 40.0%
CCTTGATTTACCTACCCTTA+TGG + chr7.3:63554287-63554306 MS.gene054918:CDS 40.0%
CTCCTTGTCTTTCTCCATAA+GGG - chr7.3:63554304-63554323 None:intergenic 40.0%
TACCCTTATGGAGAAAGACA+AGG + chr7.3:63554299-63554318 MS.gene054918:CDS 40.0%
TTGTCTTTCTCCATAAGGGT+AGG - chr7.3:63554300-63554319 None:intergenic 40.0%
!! CTTTTCCATTAACTTGGCCT+TGG - chr7.3:63554154-63554173 None:intergenic 40.0%
ACAAGGAGGTTATAAGCACC+GGG + chr7.3:63554316-63554335 MS.gene054918:CDS 45.0%
CATCGATGCCGAATTAGAGA+AGG + chr7.3:63554092-63554111 MS.gene054918:CDS 45.0%
CCTCCTTGTCTTTCTCCATA+AGG - chr7.3:63554305-63554324 None:intergenic 45.0%
CCTTATGGAGAAAGACAAGG+AGG + chr7.3:63554302-63554321 MS.gene054918:CDS 45.0%
GACAAGGAGGTTATAAGCAC+CGG + chr7.3:63554315-63554334 MS.gene054918:CDS 45.0%
GATGTTGAACGTGTTCATCG+AGG + chr7.3:63554114-63554133 MS.gene054918:CDS 45.0%
TATCCTTCGGGAATGGAATC+AGG + chr7.3:63554042-63554061 MS.gene054918:CDS 45.0%
AGGAGATGAACTTCGTGCCA+AGG + chr7.3:63554134-63554153 MS.gene054918:CDS 50.0%
CTTCCTGATTCCATTCCCGA+AGG - chr7.3:63554048-63554067 None:intergenic 50.0%
GAATGGAATCAGGAAGCTCG+TGG + chr7.3:63554052-63554071 MS.gene054918:CDS 50.0%
GCATGCTAAAGATGTCCACG+AGG + chr7.3:63554383-63554402 MS.gene054918:CDS 50.0%
TCGTGCCAAGGCCAAGTTAA+TGG + chr7.3:63554146-63554165 MS.gene054918:CDS 50.0%
! CAAGGAGGTTATAAGCACCG+GGG + chr7.3:63554317-63554336 MS.gene054918:CDS 50.0%
!! AAGGAGGTTATAAGCACCGG+GGG + chr7.3:63554318-63554337 MS.gene054918:CDS 50.0%
AAAGGAAGTCTCGAGCCTCG+TGG - chr7.3:63554401-63554420 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 63553949 63554425 63553949 ID=MS.gene054918
chr7.3 mRNA 63553949 63554425 63553949 ID=MS.gene054918.t1;Parent=MS.gene054918
chr7.3 exon 63553949 63554425 63553949 ID=MS.gene054918.t1.exon1;Parent=MS.gene054918.t1
chr7.3 CDS 63553949 63554425 63553949 ID=cds.MS.gene054918.t1;Parent=MS.gene054918.t1
Gene Sequence

>MS.gene054918

ATGACGACAGCAATAAAAAATAATAATTTGTGGCTGTTAGCGTGTTATCCATATCATCAATCCGTTTCTAGTTTCTACATCGATGGATCATTATATCCTTCGGGAATGGAATCAGGAAGCTCGTGGTTCAAGAGTTCTCATTACATCGATGCCGAATTAGAGAAGGATGTTGAACGTGTTCATCGAGGAGATGAACTTCGTGCCAAGGCCAAGTTAATGGAAAAGAAATTGCCATGGCTTGAAGTATCTAAAGTCAAAGGAAACGATGTTGTGTGTATTATAAAGAATTCAGCTATATTGACCGGGTCATTGTTCACTTTGCATGCCTCTCAAATTCACCTTGATTTACCTACCCTTATGGAGAAAGACAAGGAGGTTATAAGCACCGGGGGAGTTAAAAACAAAATTGATTTTCTGTCATTGTCATATACTAGGCATGCTAAAGATGTCCACGAGGCTCGAGACTTCCTTTCTTAG

Protein sequence

>MS.gene054918.t1

MTTAIKNNNLWLLACYPYHQSVSSFYIDGSLYPSGMESGSSWFKSSHYIDAELEKDVERVHRGDELRAKAKLMEKKLPWLEVSKVKGNDVVCIIKNSAILTGSLFTLHASQIHLDLPTLMEKDKEVISTGGVKNKIDFLSLSYTRHAKDVHEARDFLS