AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05413


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05413.t1 MTR_6g083200 93.662 142 8 1 1 141 1 142 7.05e-94 270
MS.gene05413.t1 MTR_6g083270 65.839 161 37 3 1 143 1 161 3.72e-67 210
MS.gene05413.t1 MTR_6g083310 63.462 156 43 3 1 142 1 156 8.07e-64 192
MS.gene05413.t1 MTR_6g083290 63.333 150 43 4 1 142 1 146 1.24e-57 182
MS.gene05413.t1 MTR_1g080800 52.143 140 59 3 10 143 9 146 1.07e-48 153
MS.gene05413.t1 MTR_1g080780 52.985 134 57 3 13 143 11 141 2.16e-48 152
MS.gene05413.t1 MTR_1g080820 51.449 138 59 3 10 141 9 144 1.25e-46 148
MS.gene05413.t1 MTR_1g080810 48.905 137 62 3 11 141 10 144 2.22e-44 142
MS.gene05413.t1 MTR_7g115220 52.101 119 52 2 27 142 69 185 2.17e-40 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05413.t1 AT3G04720 48.780 123 58 2 23 142 72 192 3.83e-39 131

Find 32 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATCCTCATAAAATTAAA+TGG 0.257556 6.1:-73209113 MS.gene05413:CDS
TTGTCATGGCGTAGTGAATT+TGG 0.312460 6.1:-73209039 MS.gene05413:CDS
CAGGTGACAAACAAAGAAAA+TGG 0.324037 6.1:-73208139 MS.gene05413:CDS
AAGGTAACTTTGAGCATTCC+CGG 0.379943 6.1:+73208018 None:intergenic
GAATGCTCAAAGTTACCTTA+TGG 0.411312 6.1:-73208014 MS.gene05413:CDS
CCACAAACACCTTTGCCAAC+AGG 0.421503 6.1:+73208997 None:intergenic
AAATGGAACTTGAACACATC+TGG 0.431997 6.1:-73209096 MS.gene05413:CDS
TATTGATGTGTTTCAAAAGC+TGG 0.434145 6.1:-73208053 MS.gene05413:CDS
TTTCAAAAGCTGGATAGCTC+TGG 0.449489 6.1:-73208043 MS.gene05413:CDS
GATTATGAATTTGTGGATTG+TGG 0.458700 6.1:-73207989 MS.gene05413:CDS
TATGGTGGATTATGAATTTG+TGG 0.478849 6.1:-73207996 MS.gene05413:CDS
TGGTGTGTTCTGTGCAGCTC+AGG 0.478988 6.1:-73209076 MS.gene05413:CDS
TTCTGTGGACCTGTTGGCAA+AGG 0.480910 6.1:-73209006 MS.gene05413:CDS
CAAATTCATAATCCACCATA+AGG 0.490614 6.1:+73207999 None:intergenic
TTTGGCTGGACTGCCTTCTG+TGG 0.508213 6.1:-73209021 MS.gene05413:CDS
AGGTGACAAACAAAGAAAAT+GGG 0.510288 6.1:-73208138 MS.gene05413:CDS
CCTGTTGGCAAAGGTGTTTG+TGG 0.529107 6.1:-73208997 MS.gene05413:CDS
TGTTCTGTGCAGCTCAGGAT+GGG 0.532727 6.1:-73209071 MS.gene05413:CDS
AGCTGGATAGCTCTGGGACC+GGG 0.539373 6.1:-73208036 MS.gene05413:CDS
CATGGCGTAGTGAATTTGGC+TGG 0.539611 6.1:-73209035 MS.gene05413:CDS
ACTGCCTTCTGTGGACCTGT+TGG 0.548588 6.1:-73209012 MS.gene05413:CDS
AAGCTGGATAGCTCTGGGAC+CGG 0.549261 6.1:-73208037 MS.gene05413:CDS
GTGTTCTGTGCAGCTCAGGA+TGG 0.554622 6.1:-73209072 MS.gene05413:CDS
ATCTAACTCAAGCCCTCCGT+TGG 0.561496 6.1:+73208075 None:intergenic
TTCAAAAGCTGGATAGCTCT+GGG 0.561619 6.1:-73208042 MS.gene05413:CDS
TGCTCAAAGTTACCTTATGG+TGG 0.566436 6.1:-73208011 MS.gene05413:CDS
TTGATACATGCACCAACGGA+GGG 0.588242 6.1:-73208087 MS.gene05413:CDS
ATGGGAACAAATCATTGTCA+TGG 0.598375 6.1:-73209053 MS.gene05413:CDS
TTTGCCAACAGGTCCACAGA+AGG 0.647196 6.1:+73209008 None:intergenic
TCAAAGAGCTAACAACGTGA+AGG 0.649689 6.1:-73209151 MS.gene05413:CDS
GTTGATACATGCACCAACGG+AGG 0.651216 6.1:-73208088 MS.gene05413:CDS
ATTGTTGATACATGCACCAA+CGG 0.654994 6.1:-73208091 MS.gene05413:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTTTTTTTTTTTTTA+AGG + chr6.1:73208408-73208427 None:intergenic 0.0%
!! AAAAAGTTATATATTTGTTT+GGG - chr6.1:73208872-73208891 MS.gene05413:intron 10.0%
!! AATTTCAATATAAACAAATT+TGG + chr6.1:73208674-73208693 None:intergenic 10.0%
!! AATTTACTTAAGATTTAAGA+CGG + chr6.1:73208349-73208368 None:intergenic 15.0%
!! CAAAAAGTTATATATTTGTT+TGG - chr6.1:73208871-73208890 MS.gene05413:intron 15.0%
!! TATTGTTTACTTCATATATA+TGG - chr6.1:73208973-73208992 MS.gene05413:intron 15.0%
!! TTAGTTATTCCTAATAAATA+GGG + chr6.1:73208534-73208553 None:intergenic 15.0%
!! TTATTAATCAATAAAGTTCT+AGG + chr6.1:73209009-73209028 None:intergenic 15.0%
!!! AAAAAGCATTTTACATATTA+CGG - chr6.1:73208735-73208754 MS.gene05413:intron 15.0%
!!! GCTTAAGTATTTTTTTTTTA+AGG + chr6.1:73208382-73208401 None:intergenic 15.0%
!! AATAACTAACACTATTGAAT+TGG - chr6.1:73208545-73208564 MS.gene05413:intron 20.0%
!! ACAATCCTCATAAAATTAAA+TGG - chr6.1:73208085-73208104 MS.gene05413:CDS 20.0%
!! GTTAGTTATTCCTAATAAAT+AGG + chr6.1:73208535-73208554 None:intergenic 20.0%
!! TAGTTATTCCTAATAAATAG+GGG + chr6.1:73208533-73208552 None:intergenic 20.0%
!! TATATTTACAACATCAAACT+TGG + chr6.1:73208599-73208618 None:intergenic 20.0%
!!! AAGTTCCATTTAATTTTATG+AGG + chr6.1:73208093-73208112 None:intergenic 20.0%
! CAATATAAACAAATTTGGCA+AGG + chr6.1:73208669-73208688 None:intergenic 25.0%
! CTATTGAATTGGATACTATA+AGG - chr6.1:73208556-73208575 MS.gene05413:intron 25.0%
! TGAGAGAATAAAACGATAAT+TGG + chr6.1:73208627-73208646 None:intergenic 25.0%
AGGTGACAAACAAAGAAAAT+GGG - chr6.1:73209060-73209079 MS.gene05413:CDS 30.0%
ATTCCTAATAAATAGGGGAT+CGG + chr6.1:73208528-73208547 None:intergenic 30.0%
CAAATTCATAATCCACCATA+AGG + chr6.1:73209202-73209221 None:intergenic 30.0%
CAACATCAAACTTGGTAAAT+TGG + chr6.1:73208591-73208610 None:intergenic 30.0%
TATTGATGTGTTTCAAAAGC+TGG - chr6.1:73209145-73209164 MS.gene05413:CDS 30.0%
! AGCTGGTCTAATTAGAAAAT+TGG - chr6.1:73208291-73208310 MS.gene05413:intron 30.0%
! GATTATGAATTTGTGGATTG+TGG - chr6.1:73209209-73209228 MS.gene05413:CDS 30.0%
!! ATCGTCTAATTTTGATCAAC+CGG - chr6.1:73208446-73208465 MS.gene05413:intron 30.0%
!! TATGGTGGATTATGAATTTG+TGG - chr6.1:73209202-73209221 MS.gene05413:CDS 30.0%
AAATGGAACTTGAACACATC+TGG - chr6.1:73208102-73208121 MS.gene05413:CDS 35.0%
AGCAATCAACAATGACAATG+GGG - chr6.1:73208497-73208516 MS.gene05413:intron 35.0%
ATGGGAACAAATCATTGTCA+TGG - chr6.1:73208145-73208164 MS.gene05413:intron 35.0%
ATTGTTGATACATGCACCAA+CGG - chr6.1:73209107-73209126 MS.gene05413:CDS 35.0%
CAGGTGACAAACAAAGAAAA+TGG - chr6.1:73209059-73209078 MS.gene05413:CDS 35.0%
GAATGCTCAAAGTTACCTTA+TGG - chr6.1:73209184-73209203 MS.gene05413:CDS 35.0%
GAGCAATCAACAATGACAAT+GGG - chr6.1:73208496-73208515 MS.gene05413:intron 35.0%
TCTATTGTGCTTAAATCAGC+TGG - chr6.1:73208274-73208293 MS.gene05413:intron 35.0%
TGAGCAATCAACAATGACAA+TGG - chr6.1:73208495-73208514 MS.gene05413:intron 35.0%
TGAGCTAAAACAAGACAAGA+GGG - chr6.1:73208787-73208806 MS.gene05413:intron 35.0%
TTCCTAATAAATAGGGGATC+GGG + chr6.1:73208527-73208546 None:intergenic 35.0%
!! ATTAGTCATGTACTGATTGC+TGG - chr6.1:73209029-73209048 MS.gene05413:CDS 35.0%
!! GCAGCTTTTTCTATTGGATA+AGG - chr6.1:73207990-73208009 MS.gene05413:CDS 35.0%
AAGGTAACTTTGAGCATTCC+CGG + chr6.1:73209183-73209202 None:intergenic 40.0%
ATCCCGATCCCCTATTTATT+AGG - chr6.1:73208522-73208541 MS.gene05413:intron 40.0%
GTGAGCTAAAACAAGACAAG+AGG - chr6.1:73208786-73208805 MS.gene05413:intron 40.0%
TCAAAGAGCTAACAACGTGA+AGG - chr6.1:73208047-73208066 MS.gene05413:CDS 40.0%
TGCTCAAAGTTACCTTATGG+TGG - chr6.1:73209187-73209206 MS.gene05413:CDS 40.0%
TTCAAAAGCTGGATAGCTCT+GGG - chr6.1:73209156-73209175 MS.gene05413:CDS 40.0%
TTGTCATGGCGTAGTGAATT+TGG - chr6.1:73208159-73208178 MS.gene05413:intron 40.0%
TTTCAAAAGCTGGATAGCTC+TGG - chr6.1:73209155-73209174 MS.gene05413:CDS 40.0%
!! GTCGAAGCAGCTTTTTCTAT+TGG - chr6.1:73207984-73208003 MS.gene05413:CDS 40.0%
TTGATACATGCACCAACGGA+GGG - chr6.1:73209111-73209130 MS.gene05413:CDS 45.0%
!!! TTAATATTTATTTTATTCTT+AGG - chr6.1:73208701-73208720 MS.gene05413:intron 5.0%
ACTGATTGCTGGTCGATTGC+AGG - chr6.1:73209040-73209059 MS.gene05413:CDS 50.0%
ATCTAACTCAAGCCCTCCGT+TGG + chr6.1:73209126-73209145 None:intergenic 50.0%
CATGGCGTAGTGAATTTGGC+TGG - chr6.1:73208163-73208182 MS.gene05413:intron 50.0%
CCACAAACACCTTTGCCAAC+AGG + chr6.1:73208204-73208223 None:intergenic 50.0%
GTTGATACATGCACCAACGG+AGG - chr6.1:73209110-73209129 MS.gene05413:CDS 50.0%
GTTGTTTGTGACTGCGTGAC+CGG + chr6.1:73208468-73208487 None:intergenic 50.0%
TGTTCTGTGCAGCTCAGGAT+GGG - chr6.1:73208127-73208146 MS.gene05413:CDS 50.0%
TTCTGTGGACCTGTTGGCAA+AGG - chr6.1:73208192-73208211 MS.gene05413:intron 50.0%
TTTGCCAACAGGTCCACAGA+AGG + chr6.1:73208193-73208212 None:intergenic 50.0%
!! CCTGTTGGCAAAGGTGTTTG+TGG - chr6.1:73208201-73208220 MS.gene05413:intron 50.0%
ACTGCCTTCTGTGGACCTGT+TGG - chr6.1:73208186-73208205 MS.gene05413:intron 55.0%
GTGTTCTGTGCAGCTCAGGA+TGG - chr6.1:73208126-73208145 MS.gene05413:CDS 55.0%
TGGTGTGTTCTGTGCAGCTC+AGG - chr6.1:73208122-73208141 MS.gene05413:CDS 55.0%
TTTGGCTGGACTGCCTTCTG+TGG - chr6.1:73208177-73208196 MS.gene05413:intron 55.0%
!! AAGCTGGATAGCTCTGGGAC+CGG - chr6.1:73209161-73209180 MS.gene05413:CDS 55.0%
!! AGCTGGATAGCTCTGGGACC+GGG - chr6.1:73209162-73209181 MS.gene05413:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 73207982 73209238 73207982 ID=MS.gene05413
chr6.1 mRNA 73207982 73209238 73207982 ID=MS.gene05413.t1;Parent=MS.gene05413
chr6.1 exon 73208984 73209238 73208984 ID=MS.gene05413.t1.exon1;Parent=MS.gene05413.t1
chr6.1 CDS 73208984 73209238 73208984 ID=cds.MS.gene05413.t1;Parent=MS.gene05413.t1
chr6.1 exon 73207982 73208158 73207982 ID=MS.gene05413.t1.exon2;Parent=MS.gene05413.t1
chr6.1 CDS 73207982 73208158 73207982 ID=cds.MS.gene05413.t1;Parent=MS.gene05413.t1
Gene Sequence

>MS.gene05413

ATGTCGAAGCAGCTTTTTCTATTGGATAAGGTGTTGATATTTTGTGTTCTAGCACTTGCATCTGCTCAAAGAGCTAACAACGTGAAGGCTACATATAAATCATACAATCCTCATAAAATTAAATGGAACTTGAACACATCTGGTGTGTTCTGTGCAGCTCAGGATGGGAACAAATCATTGTCATGGCGTAGTGAATTTGGCTGGACTGCCTTCTGTGGACCTGTTGGCAAAGGTGTTTGTGGCAAGTGCTTAAATGTATGTATGCATATGCTTTTGTTACTTTAGCTGTTTTTCTATTGTGCTTAAATCAGCTGGTCTAATTAGAAAATTGGATTCTCATTCACGCAGTCAAATGATTGCGTGACCGTCTTAAATCTTAAGTAAATTTAATTTTTTTCCTTAAAAAAAAAATACTTAAGCTTTCCTTAAAAAAAAAAAAAATAATACTTAAGCTTGAGATTTAAATCGTCTAATTTTGATCAACCGGTCACGCAGTCACAAACAACATAAATGTGAGCAATCAACAATGACAATGGGGAAATCCCGATCCCCTATTTATTAGGAATAACTAACACTATTGAATTGGATACTATAAGGACTAAATTACCAATTTACCAAGTTTGATGTTGTAAATATAAATTACCAATTATCGTTTTATTCTCTCAATTACATAATTTTTTTAGTCCTTGCCAAATTTGTTTATATTGAAATTGATCTTTTTAATATTTATTTTATTCTTAGGAACTAATTTAAAAAAAGCATTTTACATATTACGGAGATCAATTTAATATGCATAAATTTTGTGTGAGCTAAAACAAGACAAGAGGGACGAAAAGAAAAAAGTTATATATTTACAAGAACTAAAAGTAAAATATTATAGAGATTAAAACAAAAAGTTATATATTTGTTTGGGCTAAAAAAATTAAACTTCAATATTTTCAAATAAATTTAAAATATTGTTCACTTCATATATTTTAAAATAAATTTAAAATATTGTTTACTTCATATATATGGTTGATAATAGCCTAGAACTTTATTGATTAATAAATTAGTCATGTACTGATTGCTGGTCGATTGCAGGTGACAAACAAAGAAAATGGGATTATAGTCTCTAAAATAGTAAGAATTGTTGATACATGCACCAACGGAGGGCTTGAGTTAGATATTGATGTGTTTCAAAAGCTGGATAGCTCTGGGACCGGGAATGCTCAAAGTTACCTTATGGTGGATTATGAATTTGTGGATTGTGGTGAGTAG

Protein sequence

>MS.gene05413.t1

MSKQLFLLDKVLIFCVLALASAQRANNVKATYKSYNPHKIKWNLNTSGVFCAAQDGNKSLSWRSEFGWTAFCGPVGKGVCGKCLNVTNKENGIIVSKIVRIVDTCTNGGLELDIDVFQKLDSSGTGNAQSYLMVDYEFVDCGE