AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066758

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066758.t1 MTR_6g047800 97.735 309 6 1 1 308 1 309 0.0 624
MS.gene066758.t1 MTR_6g047800 98.000 300 5 1 10 308 1 300 0.0 607
MS.gene066758.t1 MTR_2g014590 77.056 231 53 0 1 231 2 232 1.83e-137 389
MS.gene066758.t1 MTR_2g014570 70.968 62 13 3 246 307 6 62 5.81e-19 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066758.t1 AT5G14600 73.968 315 75 2 1 308 1 315 3.93e-176 489

Find 69 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTTGTTGTTATGTATT+TGG 0.132518 2.2:+28551730 MS.gene066758:CDS
TCCTAATTCTTCTTCTGTTA+TGG 0.259735 2.2:+28553776 MS.gene066758:CDS
AACAATTAGTAACGGTGATT+TGG 0.271982 2.2:+28551481 MS.gene066758:CDS
TCCTGAAGAATTTGCTGGCT+TGG 0.291582 2.2:+28553323 MS.gene066758:CDS
CGAAGTTCGAGAAGGGAAAA+TGG 0.297664 2.2:+28553662 MS.gene066758:CDS
TTGTTTATGTGAAAGATATA+AGG 0.305535 2.2:+28553613 MS.gene066758:intron
GGTTGTGTCGTTCTTGAATC+TGG 0.320717 2.2:+28551764 MS.gene066758:CDS
AAAACTAAAGTCCACAATTC+AGG 0.328439 2.2:-28551671 None:intergenic
GATTGGATAGGAAAGTCATT+TGG 0.331486 2.2:+28551596 MS.gene066758:CDS
GTCTAAAGTATTCAGCAATA+AGG 0.337791 2.2:+28551619 MS.gene066758:CDS
GCCAAGCCAGCAAATTCTTC+AGG 0.357310 2.2:-28553324 None:intergenic
GCCATAACAGAAGAAGAATT+AGG 0.368158 2.2:-28553777 None:intergenic
GATCCCTCAGACACCGTTAC+TGG 0.378943 2.2:-28551536 None:intergenic
GGGTTTCCTGAAGAATTTGC+TGG 0.381718 2.2:+28553318 MS.gene066758:CDS
ATGTATTTGGAGATTGTTCC+TGG 0.385811 2.2:+28551743 MS.gene066758:CDS
AGGGATGACTTTGAGAAGAT+TGG 0.392112 2.2:+28553249 MS.gene066758:intron
AACGGTGATGACGGCTCTAA+TGG 0.392261 2.2:+28553699 MS.gene066758:CDS
CTTGAATCTGGTACTGGAAG+TGG 0.400748 2.2:+28551776 MS.gene066758:CDS
ATTGGATAGGAAAGTCATTT+GGG 0.422230 2.2:+28551597 MS.gene066758:CDS
TTGCAAGAGGAGGCAGCGTT+CGG 0.450518 2.2:+28553731 MS.gene066758:CDS
ATGGAAAGCTTAGAGATTAA+CGG 0.454257 2.2:+28553681 MS.gene066758:CDS
TCTAAAGTATTCAGCAATAA+GGG 0.459899 2.2:+28551620 MS.gene066758:CDS
GTCGTTCTTGAATCTGGTAC+TGG 0.478619 2.2:+28551770 MS.gene066758:CDS
CTTTAACTACTTCACTTGCA+AGG 0.505822 2.2:+28551801 MS.gene066758:CDS
AGGGGAAGTACCGGTGAAGT+TGG 0.508203 2.2:-28553484 None:intergenic
ATAAGCAGCTTAGTCACTGC+TGG 0.509748 2.2:+28553273 MS.gene066758:CDS
GCAAGGGCAGTTGCTCCTAC+AGG 0.518867 2.2:+28551818 MS.gene066758:CDS
GCTGGAGTGAGGGACATTCA+AGG 0.529002 2.2:+28553291 MS.gene066758:CDS
TTGAATCTGGTACTGGAAGT+GGG 0.536848 2.2:+28551777 MS.gene066758:CDS
CGTTGTACTTGTTCAATACA+CGG 0.539892 2.2:-28553441 None:intergenic
GAAGCCAGTAACGGTGTCTG+AGG 0.543073 2.2:+28551532 MS.gene066758:CDS
ATTTGGCAATTGTTTATGCA+AGG 0.545176 2.2:+28551498 MS.gene066758:CDS
TTCAAACATTCAGATTGGAT+AGG 0.552647 2.2:+28551584 MS.gene066758:CDS
GAACGCTGCCTCCTCTTGCA+AGG 0.565950 2.2:-28553729 None:intergenic
AGTAACGGTGTCTGAGGGAT+CGG 0.566481 2.2:+28551538 MS.gene066758:CDS
AAGAGGAGGCAGCGTTCGGA+TGG 0.567518 2.2:+28553735 MS.gene066758:CDS
GTGAGGGACATTCAAGGTGA+GGG 0.570540 2.2:+28553297 MS.gene066758:CDS
GGAATAGCTAGCCAAGGTTG+AGG 0.572196 2.2:-28553369 None:intergenic
TAGCACCTACTCCTGAATTG+TGG 0.573848 2.2:+28551660 MS.gene066758:CDS
ATGGTTCTCTTCCTTGCAAG+AGG 0.575002 2.2:+28553718 MS.gene066758:CDS
TTTAACTACTTCACTTGCAA+GGG 0.578833 2.2:+28551802 MS.gene066758:CDS
ACAATGCAATCCAACTTCAC+CGG 0.584109 2.2:+28553474 MS.gene066758:CDS
GTTCTCTTCCTTGCAAGAGG+AGG 0.584259 2.2:+28553721 MS.gene066758:CDS
GTTGTACTTGTTCAATACAC+GGG 0.584848 2.2:-28553440 None:intergenic
GATTCAAGAACGACACAACC+AGG 0.585429 2.2:-28551761 None:intergenic
CTTAGTCACTGCTGGAGTGA+GGG 0.585877 2.2:+28553281 MS.gene066758:CDS
TTAGAGATTAACGGTGATGA+CGG 0.592052 2.2:+28553690 MS.gene066758:CDS
AAAGTCCACAATTCAGGAGT+AGG 0.594923 2.2:-28551665 None:intergenic
TTTAATAGAACAATTAGTAA+CGG 0.596180 2.2:+28551473 MS.gene066758:CDS
GCTTAGTCACTGCTGGAGTG+AGG 0.596199 2.2:+28553280 MS.gene066758:CDS
AACGTATAAACATGTCCTGT+AGG 0.611682 2.2:-28551833 None:intergenic
CGGACATACGAAGTTCGAGA+AGG 0.611846 2.2:+28553654 MS.gene066758:CDS
CTCGAACTTCGTATGTCCGT+AGG 0.612530 2.2:-28553650 None:intergenic
TGACCTCTAGCTTCACCACA+TGG 0.618356 2.2:-28553804 None:intergenic
GGACATACGAAGTTCGAGAA+GGG 0.625372 2.2:+28553655 MS.gene066758:CDS
TGAGGGACATTCAAGGTGAG+GGG 0.627956 2.2:+28553298 MS.gene066758:CDS
GCGTTCGGATGGAGCTAGTG+TGG 0.628213 2.2:+28553746 MS.gene066758:CDS
TCTGTTATGGCTAGACCATG+TGG 0.628693 2.2:+28553789 MS.gene066758:CDS
AACAGAAGAAGAATTAGGAT+AGG 0.630818 2.2:-28553772 None:intergenic
AACAAAGATCTGGATCAGCT+AGG 0.636973 2.2:+28551867 MS.gene066758:CDS
AAGCCAGTAACGGTGTCTGA+GGG 0.637458 2.2:+28551533 MS.gene066758:CDS
AGACCATGTGGTGAAGCTAG+AGG 0.638065 2.2:+28553801 MS.gene066758:CDS
GCTGAAGGAATAGCTAGCCA+AGG 0.647664 2.2:-28553375 None:intergenic
CATATAGAATCTGTGTCCTG+TGG 0.649958 2.2:-28551697 None:intergenic
AGTGAGGGACATTCAAGGTG+AGG 0.655644 2.2:+28553296 MS.gene066758:CDS
TGACAACATGAAGCCAGTAA+CGG 0.667095 2.2:+28551523 MS.gene066758:CDS
GGTGAAGCTAGAGGTCACAC+TGG 0.683965 2.2:+28553810 MS.gene066758:CDS
AAAGTATTCAGCAATAAGGG+CGG 0.684692 2.2:+28551623 MS.gene066758:CDS
TTGTACTTGTTCAATACACG+GGG 0.704021 2.2:-28553439 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATATAAAAGAAGAAAAA+AGG - chr2.2:28553507-28553526 None:intergenic 15.0%
!! ACATATAAAAGAAGAAAAAA+GGG - chr2.2:28553506-28553525 None:intergenic 15.0%
!! AGTCAATTATATATAAAACA+AGG - chr2.2:28552943-28552962 None:intergenic 15.0%
!! ATAGCTTATATATTGTATTA+CGG - chr2.2:28552881-28552900 None:intergenic 15.0%
!! TTTAATAGAACAATTAGTAA+CGG + chr2.2:28551473-28551492 MS.gene066758:CDS 15.0%
!! ATAATATTACTTGCAATTAC+TGG - chr2.2:28553093-28553112 None:intergenic 20.0%
!! ATATCTTTCACATAAACAAA+CGG - chr2.2:28553612-28553631 None:intergenic 20.0%
!! CATACAAATAAAAAAAGGAA+TGG - chr2.2:28552913-28552932 None:intergenic 20.0%
!! CATATAAAAGAAGAAAAAAG+GGG - chr2.2:28553505-28553524 None:intergenic 20.0%
!! GTAATTGCAAGTAATATTAT+TGG + chr2.2:28553093-28553112 MS.gene066758:intron 20.0%
!! TTGTTTATGTGAAAGATATA+AGG + chr2.2:28553613-28553632 MS.gene066758:intron 20.0%
!! TTTCTAATGTTGTCAAATAT+TGG + chr2.2:28551965-28551984 MS.gene066758:intron 20.0%
!!! AATATTGTTTTTCTTCTTGT+AGG + chr2.2:28553229-28553248 MS.gene066758:intron 20.0%
!!! ATATTGTTTTTCTTCTTGTA+GGG + chr2.2:28553230-28553249 MS.gene066758:intron 20.0%
!!! CCTTTTTTTATTTGTATGTA+GGG + chr2.2:28552915-28552934 MS.gene066758:intron 20.0%
!!! TCCTTTTTTTATTTGTATGT+AGG + chr2.2:28552914-28552933 MS.gene066758:intron 20.0%
!!! TTTTTACAATGGAAAAACTT+AGG + chr2.2:28552828-28552847 MS.gene066758:intron 20.0%
! ACAAATAAAAAAAGGAATGG+AGG - chr2.2:28552910-28552929 None:intergenic 25.0%
! ATATACATGTGCTTATCTTT+AGG + chr2.2:28551924-28551943 MS.gene066758:intron 25.0%
! CCCTACATACAAATAAAAAA+AGG - chr2.2:28552918-28552937 None:intergenic 25.0%
! CTACCAAAAAACTAGAATAA+CGG - chr2.2:28552054-28552073 None:intergenic 25.0%
! TGAAATAGAAGATAGTAACA+CGG - chr2.2:28552999-28553018 None:intergenic 25.0%
!! TCTAAAGTATTCAGCAATAA+GGG + chr2.2:28551620-28551639 MS.gene066758:CDS 25.0%
!! TTGTCAAATTGATTCAATAG+CGG + chr2.2:28552611-28552630 MS.gene066758:intron 25.0%
!!! TCACCAAAAATACGTTTTTT+AGG - chr2.2:28552171-28552190 None:intergenic 25.0%
AAAACTAAAGTCCACAATTC+AGG - chr2.2:28551674-28551693 None:intergenic 30.0%
AAAATCACGATCAAACTCAT+GGG - chr2.2:28552367-28552386 None:intergenic 30.0%
AACAATTAGTAACGGTGATT+TGG + chr2.2:28551481-28551500 MS.gene066758:CDS 30.0%
AACAGAAGAAGAATTAGGAT+AGG - chr2.2:28553775-28553794 None:intergenic 30.0%
ACATAAACAAACGGATACAA+AGG - chr2.2:28553603-28553622 None:intergenic 30.0%
ATGGAAAGCTTAGAGATTAA+CGG + chr2.2:28553681-28553700 MS.gene066758:CDS 30.0%
ATTGGATAGGAAAGTCATTT+GGG + chr2.2:28551597-28551616 MS.gene066758:CDS 30.0%
ATTGTAAAAATAAGCCACGT+TGG - chr2.2:28552818-28552837 None:intergenic 30.0%
ATTTGGCAATTGTTTATGCA+AGG + chr2.2:28551498-28551517 MS.gene066758:CDS 30.0%
GAAATAGAAGATAGTAACAC+GGG - chr2.2:28552998-28553017 None:intergenic 30.0%
GACTTATTTCAACTGTTATG+CGG + chr2.2:28552959-28552978 MS.gene066758:intron 30.0%
TATTGTATTACGGTGAATAC+AGG - chr2.2:28552871-28552890 None:intergenic 30.0%
TCCTAATTCTTCTTCTGTTA+TGG + chr2.2:28553776-28553795 MS.gene066758:CDS 30.0%
TTCAAACATTCAGATTGGAT+AGG + chr2.2:28551584-28551603 MS.gene066758:CDS 30.0%
TTGTAAAAATAAGCCACGTT+GGG - chr2.2:28552817-28552836 None:intergenic 30.0%
TTTAACTACTTCACTTGCAA+GGG + chr2.2:28551802-28551821 MS.gene066758:CDS 30.0%
TTTCCGTAATATGCTATTGA+TGG + chr2.2:28553028-28553047 MS.gene066758:intron 30.0%
! AGCAGAAAAAAGAGACTTTT+AGG + chr2.2:28552090-28552109 MS.gene066758:intron 30.0%
! ATTTGATTTCCATGAGCATA+GGG + chr2.2:28552497-28552516 MS.gene066758:intron 30.0%
! GAAAGATATAAGGACTTTTG+AGG + chr2.2:28553623-28553642 MS.gene066758:intron 30.0%
! GTCTAAAGTATTCAGCAATA+AGG + chr2.2:28551619-28551638 MS.gene066758:CDS 30.0%
! GTGTTTTCAAACATTCAGAT+TGG + chr2.2:28551579-28551598 MS.gene066758:CDS 30.0%
!! ATCTTGCAATTCATTGATTC+AGG - chr2.2:28552243-28552262 None:intergenic 30.0%
!! CAGCTTTGTTGTTATGTATT+TGG + chr2.2:28551730-28551749 MS.gene066758:CDS 30.0%
!!! AAACGTATTTTTGGTGAAAC+TGG + chr2.2:28552174-28552193 MS.gene066758:intron 30.0%
!!! AACGTATTTTTGGTGAAACT+GGG + chr2.2:28552175-28552194 MS.gene066758:intron 30.0%
!!! AACGTGGCTTATTTTTACAA+TGG + chr2.2:28552817-28552836 MS.gene066758:intron 30.0%
!!! AGTGTTGCAGTTTTTTTTAG+CGG - chr2.2:28552019-28552038 None:intergenic 30.0%
!!! CCAAAAATACGTTTTTTAGG+CGG - chr2.2:28552168-28552187 None:intergenic 30.0%
!!! TTGCCGTTATTCTAGTTTTT+TGG + chr2.2:28552048-28552067 MS.gene066758:intron 30.0%
AACGTATAAACATGTCCTGT+AGG - chr2.2:28551836-28551855 None:intergenic 35.0%
ACACATGTATCAAACATCAG+AGG - chr2.2:28552552-28552571 None:intergenic 35.0%
ACTTGAGATGTTCAGTGTAA+AGG + chr2.2:28553191-28553210 MS.gene066758:intron 35.0%
ATAAACATGCCCTTCACAAA+TGG - chr2.2:28552475-28552494 None:intergenic 35.0%
ATCCCTTCATAAATTGGCTA+TGG + chr2.2:28552409-28552428 MS.gene066758:intron 35.0%
ATGTATTTGGAGATTGTTCC+TGG + chr2.2:28551743-28551762 MS.gene066758:CDS 35.0%
CAAAATCACGATCAAACTCA+TGG - chr2.2:28552368-28552387 None:intergenic 35.0%
CAATGGAAAAACTTAGGAGT+CGG + chr2.2:28552834-28552853 MS.gene066758:intron 35.0%
CGTAGTAGTTATGCAACTAA+TGG + chr2.2:28553550-28553569 MS.gene066758:intron 35.0%
CGTTGTACTTGTTCAATACA+CGG - chr2.2:28553444-28553463 None:intergenic 35.0%
CTATCTTCTATTTCACGTAG+CGG + chr2.2:28553004-28553023 MS.gene066758:intron 35.0%
CTTTAACTACTTCACTTGCA+AGG + chr2.2:28551801-28551820 MS.gene066758:CDS 35.0%
GATTGGATAGGAAAGTCATT+TGG + chr2.2:28551596-28551615 MS.gene066758:CDS 35.0%
GCAAAAATGTTACGACATGA+CGG + chr2.2:28553399-28553418 MS.gene066758:CDS 35.0%
GCCATAACAGAAGAAGAATT+AGG - chr2.2:28553780-28553799 None:intergenic 35.0%
GGTTATCTTCCTTGAAATTC+CGG - chr2.2:28552222-28552241 None:intergenic 35.0%
GTGCCATCAATAGCATATTA+CGG - chr2.2:28553034-28553053 None:intergenic 35.0%
GTTATCTTCCTTGAAATTCC+GGG - chr2.2:28552221-28552240 None:intergenic 35.0%
GTTGTACTTGTTCAATACAC+GGG - chr2.2:28553443-28553462 None:intergenic 35.0%
TTAGAGATTAACGGTGATGA+CGG + chr2.2:28553690-28553709 MS.gene066758:CDS 35.0%
TTGTACTTGTTCAATACACG+GGG - chr2.2:28553442-28553461 None:intergenic 35.0%
! AAAGTATTCAGCAATAAGGG+CGG + chr2.2:28551623-28551642 MS.gene066758:CDS 35.0%
! CATTTGATTTCCATGAGCAT+AGG + chr2.2:28552496-28552515 MS.gene066758:intron 35.0%
! CCGCCTAAAAAACGTATTTT+TGG + chr2.2:28552165-28552184 MS.gene066758:intron 35.0%
! GATTTTCACGAACAAAGATC+TGG + chr2.2:28551857-28551876 MS.gene066758:CDS 35.0%
! TCGGTTTTACAGAATCGATT+CGG + chr2.2:28551557-28551576 MS.gene066758:CDS 35.0%
!!! ATACGTTTTTTAGGCGGTAA+TGG - chr2.2:28552162-28552181 None:intergenic 35.0%
AAAGTCCACAATTCAGGAGT+AGG - chr2.2:28551668-28551687 None:intergenic 40.0%
AACAAAGATCTGGATCAGCT+AGG + chr2.2:28551867-28551886 MS.gene066758:CDS 40.0%
ACAATGCAATCCAACTTCAC+CGG + chr2.2:28553474-28553493 MS.gene066758:CDS 40.0%
ACCGCAGAAAATTGTAGGTA+CGG - chr2.2:28552270-28552289 None:intergenic 40.0%
ACTATTGTGCTATCACATGG+TGG + chr2.2:28552635-28552654 MS.gene066758:intron 40.0%
ATATTGGCGCTATAGCATAG+CGG + chr2.2:28551981-28552000 MS.gene066758:intron 40.0%
ATCAAACATCAGAGGAAGTC+TGG - chr2.2:28552544-28552563 None:intergenic 40.0%
CATATAGAATCTGTGTCCTG+TGG - chr2.2:28551700-28551719 None:intergenic 40.0%
CCTTCATAAATTGGCTATGG+CGG + chr2.2:28552412-28552431 MS.gene066758:intron 40.0%
CGCCATAGCCAATTTATGAA+GGG - chr2.2:28552414-28552433 None:intergenic 40.0%
CTTCATAAATTGGCTATGGC+GGG + chr2.2:28552413-28552432 MS.gene066758:intron 40.0%
GTATGGTCGGAATCAGAATT+TGG - chr2.2:28552310-28552329 None:intergenic 40.0%
TGACAACATGAAGCCAGTAA+CGG + chr2.2:28551523-28551542 MS.gene066758:CDS 40.0%
TTAGGCTGTGTTGTCAATTG+TGG + chr2.2:28552108-28552127 MS.gene066758:intron 40.0%
! AGGGATGACTTTGAGAAGAT+TGG + chr2.2:28553249-28553268 MS.gene066758:intron 40.0%
! CGTAACATTTTTGCAGCTGA+AGG - chr2.2:28553393-28553412 None:intergenic 40.0%
! GAAGAAAAAAGGGGAAGTAC+CGG - chr2.2:28553496-28553515 None:intergenic 40.0%
! TGCTATTGATGGCACTTTCA+TGG + chr2.2:28553039-28553058 MS.gene066758:intron 40.0%
! TTGAATCTGGTACTGGAAGT+GGG + chr2.2:28551777-28551796 MS.gene066758:CDS 40.0%
! TTTTGGATCTACCTCAACCT+TGG + chr2.2:28553358-28553377 MS.gene066758:CDS 40.0%
!!! CTTGGCTGATGCAGTTTTTT+TGG + chr2.2:28553341-28553360 MS.gene066758:CDS 40.0%
!!! GATCGTGATTTTGACTTCCT+TGG + chr2.2:28552375-28552394 MS.gene066758:intron 40.0%
!!! GGACTTTAGTTTTAAGCCAC+AGG + chr2.2:28551681-28551700 MS.gene066758:CDS 40.0%
AACATGCCCTTCACAAATGG+TGG - chr2.2:28552472-28552491 None:intergenic 45.0%
ATAAGCAGCTTAGTCACTGC+TGG + chr2.2:28553273-28553292 MS.gene066758:CDS 45.0%
ATGATCCCCTATGAAAACCG+CGG + chr2.2:28552664-28552683 MS.gene066758:intron 45.0%
ATGGTTCTCTTCCTTGCAAG+AGG + chr2.2:28553718-28553737 MS.gene066758:CDS 45.0%
CCGCCATAGCCAATTTATGA+AGG - chr2.2:28552415-28552434 None:intergenic 45.0%
CGAAGTTCGAGAAGGGAAAA+TGG + chr2.2:28553662-28553681 MS.gene066758:CDS 45.0%
CTATCCCAGTTTCGCTATCA+GGG + chr2.2:28552689-28552708 MS.gene066758:intron 45.0%
GATTCAAGAACGACACAACC+AGG - chr2.2:28551764-28551783 None:intergenic 45.0%
GCAGAAAATTGTAGGTACGG+CGG - chr2.2:28552267-28552286 None:intergenic 45.0%
GCAGGCATCCCTTCATAAAT+TGG + chr2.2:28552403-28552422 MS.gene066758:intron 45.0%
GCATCACCGCAGAAAATTGT+AGG - chr2.2:28552275-28552294 None:intergenic 45.0%
GCTGTGTTGTCAATTGTGGA+AGG + chr2.2:28552112-28552131 MS.gene066758:intron 45.0%
GGACATACGAAGTTCGAGAA+GGG + chr2.2:28553655-28553674 MS.gene066758:CDS 45.0%
GGCACTATTGTGCTATCACA+TGG + chr2.2:28552632-28552651 MS.gene066758:intron 45.0%
GGGTTTCCTGAAGAATTTGC+TGG + chr2.2:28553318-28553337 MS.gene066758:CDS 45.0%
GGTCGGAATCAGAATTTGGT+GGG - chr2.2:28552306-28552325 None:intergenic 45.0%
GTCGTTCTTGAATCTGGTAC+TGG + chr2.2:28551770-28551789 MS.gene066758:CDS 45.0%
TAGCACCTACTCCTGAATTG+TGG + chr2.2:28551660-28551679 MS.gene066758:CDS 45.0%
TCTGTTATGGCTAGACCATG+TGG + chr2.2:28553789-28553808 MS.gene066758:CDS 45.0%
TGGTCGGAATCAGAATTTGG+TGG - chr2.2:28552307-28552326 None:intergenic 45.0%
TTTCGCTATCAGGGCAATAG+CGG + chr2.2:28552698-28552717 MS.gene066758:intron 45.0%
! ACAATTTTCTGCGGTGATGC+TGG + chr2.2:28552275-28552294 MS.gene066758:intron 45.0%
! ATTGCCCTGATAGCGAAACT+GGG - chr2.2:28552696-28552715 None:intergenic 45.0%
! CTTGAATCTGGTACTGGAAG+TGG + chr2.2:28551776-28551795 MS.gene066758:CDS 45.0%
! GCCGTACCTACAATTTTCTG+CGG + chr2.2:28552266-28552285 MS.gene066758:intron 45.0%
! GGTTGTGTCGTTCTTGAATC+TGG + chr2.2:28551764-28551783 MS.gene066758:CDS 45.0%
! TATTGCCCTGATAGCGAAAC+TGG - chr2.2:28552697-28552716 None:intergenic 45.0%
!! TCCTGAAGAATTTGCTGGCT+TGG + chr2.2:28553323-28553342 MS.gene066758:CDS 45.0%
!! TTTTTGGTGAAACTGGGTCC+TGG + chr2.2:28552181-28552200 MS.gene066758:intron 45.0%
!!! GATTTTGACTTCCTTGGCCA+TGG + chr2.2:28552381-28552400 MS.gene066758:intron 45.0%
!!! GGACTTTTGAGGTACTCCTA+CGG + chr2.2:28553634-28553653 MS.gene066758:CDS 45.0%
AAGCCAGTAACGGTGTCTGA+GGG + chr2.2:28551533-28551552 MS.gene066758:CDS 50.0%
ACCATACTCCAGCATGCCAA+GGG + chr2.2:28552323-28552342 MS.gene066758:intron 50.0%
AGACCATGTGGTGAAGCTAG+AGG + chr2.2:28553801-28553820 MS.gene066758:CDS 50.0%
AGTAACGGTGTCTGAGGGAT+CGG + chr2.2:28551538-28551557 MS.gene066758:CDS 50.0%
AGTGAGGGACATTCAAGGTG+AGG + chr2.2:28553296-28553315 MS.gene066758:CDS 50.0%
CGGACATACGAAGTTCGAGA+AGG + chr2.2:28553654-28553673 MS.gene066758:CDS 50.0%
CTCGAACTTCGTATGTCCGT+AGG - chr2.2:28553653-28553672 None:intergenic 50.0%
CTTAGTCACTGCTGGAGTGA+GGG + chr2.2:28553281-28553300 MS.gene066758:CDS 50.0%
GATTTCCATGAGCATAGGGC+TGG + chr2.2:28552501-28552520 MS.gene066758:intron 50.0%
GCCAAGCCAGCAAATTCTTC+AGG - chr2.2:28553327-28553346 None:intergenic 50.0%
GCTATCCCAGTTTCGCTATC+AGG + chr2.2:28552688-28552707 MS.gene066758:intron 50.0%
GCTGAAGGAATAGCTAGCCA+AGG - chr2.2:28553378-28553397 None:intergenic 50.0%
GGAATAGCTAGCCAAGGTTG+AGG - chr2.2:28553372-28553391 None:intergenic 50.0%
GTGAGGGACATTCAAGGTGA+GGG + chr2.2:28553297-28553316 MS.gene066758:CDS 50.0%
GTTCTCTTCCTTGCAAGAGG+AGG + chr2.2:28553721-28553740 MS.gene066758:CDS 50.0%
TCCTTGAAATTCCGGGTTCG+CGG - chr2.2:28552214-28552233 None:intergenic 50.0%
TGAGGGACATTCAAGGTGAG+GGG + chr2.2:28553298-28553317 MS.gene066758:CDS 50.0%
TTATGAAGGGATGCCTGCCA+TGG - chr2.2:28552401-28552420 None:intergenic 50.0%
TTGGCATGCTGGAGTATGGT+CGG - chr2.2:28552323-28552342 None:intergenic 50.0%
! ATAGCGCCGCGGTTTTCATA+GGG - chr2.2:28552673-28552692 None:intergenic 50.0%
! CGCATGATTTTGCTACCCCT+TGG - chr2.2:28552342-28552361 None:intergenic 50.0%
! TGACCTCTAGCTTCACCACA+TGG - chr2.2:28553807-28553826 None:intergenic 50.0%
!! AACGGTGATGACGGCTCTAA+TGG + chr2.2:28553699-28553718 MS.gene066758:CDS 50.0%
AAATTCCGGGTTCGCGGGTT+GGG - chr2.2:28552208-28552227 None:intergenic 55.0%
ACCGTGGCCACCATTTGTGA+AGG + chr2.2:28552462-28552481 MS.gene066758:intron 55.0%
ATCTGCATGTGTGCCCAACG+TGG + chr2.2:28552801-28552820 MS.gene066758:intron 55.0%
CCATACTCCAGCATGCCAAG+GGG + chr2.2:28552324-28552343 MS.gene066758:intron 55.0%
CCCTTCACAAATGGTGGCCA+CGG - chr2.2:28552466-28552485 None:intergenic 55.0%
CCGTGGCCACCATTTGTGAA+GGG + chr2.2:28552463-28552482 MS.gene066758:intron 55.0%
CCTTGAAATTCCGGGTTCGC+GGG - chr2.2:28552213-28552232 None:intergenic 55.0%
CTCATCCAGCCCTATGCTCA+TGG - chr2.2:28552509-28552528 None:intergenic 55.0%
GAAGCCAGTAACGGTGTCTG+AGG + chr2.2:28551532-28551551 MS.gene066758:CDS 55.0%
GACCATACTCCAGCATGCCA+AGG + chr2.2:28552322-28552341 MS.gene066758:intron 55.0%
GATCCCTCAGACACCGTTAC+TGG - chr2.2:28551539-28551558 None:intergenic 55.0%
GCTGGAGTGAGGGACATTCA+AGG + chr2.2:28553291-28553310 MS.gene066758:CDS 55.0%
GCTTAGTCACTGCTGGAGTG+AGG + chr2.2:28553280-28553299 MS.gene066758:CDS 55.0%
GGTGAAGCTAGAGGTCACAC+TGG + chr2.2:28553810-28553829 MS.gene066758:CDS 55.0%
TCTGCCTCACAATTCCACCG+TGG + chr2.2:28552446-28552465 MS.gene066758:intron 55.0%
TTCACAAATGGTGGCCACGG+TGG - chr2.2:28552463-28552482 None:intergenic 55.0%
TTGACTTCCTTGGCCATGGC+AGG + chr2.2:28552385-28552404 MS.gene066758:intron 55.0%
TTGCAAGAGGAGGCAGCGTT+CGG + chr2.2:28553731-28553750 MS.gene066758:CDS 55.0%
TTTGCTACCCCTTGGCATGC+TGG - chr2.2:28552334-28552353 None:intergenic 55.0%
! GATAGCGCCGCGGTTTTCAT+AGG - chr2.2:28552674-28552693 None:intergenic 55.0%
! TAGCGCCGCGGTTTTCATAG+GGG - chr2.2:28552672-28552691 None:intergenic 55.0%
!! AGGGGAAGTACCGGTGAAGT+TGG - chr2.2:28553487-28553506 None:intergenic 55.0%
AAGAGGAGGCAGCGTTCGGA+TGG + chr2.2:28553735-28553754 MS.gene066758:CDS 60.0%
CCCCTTGGCATGCTGGAGTA+TGG - chr2.2:28552327-28552346 None:intergenic 60.0%
CCCGCGAACCCGGAATTTCA+AGG + chr2.2:28552210-28552229 MS.gene066758:intron 60.0%
GAAATTCCGGGTTCGCGGGT+TGG - chr2.2:28552209-28552228 None:intergenic 60.0%
GAACGCTGCCTCCTCTTGCA+AGG - chr2.2:28553732-28553751 None:intergenic 60.0%
GCAAGGGCAGTTGCTCCTAC+AGG + chr2.2:28551818-28551837 MS.gene066758:CDS 60.0%
GCGTTCGGATGGAGCTAGTG+TGG + chr2.2:28553746-28553765 MS.gene066758:CDS 60.0%
GTGGCCACGGTGGAATTGTG+AGG - chr2.2:28552453-28552472 None:intergenic 60.0%
AGGGATGCCTGCCATGGCCA+AGG - chr2.2:28552395-28552414 None:intergenic 65.0%
GCGAAACTGGGATAGCGCCG+CGG - chr2.2:28552684-28552703 None:intergenic 65.0%
CTGGACCCAACCCGCGAACC+CGG + chr2.2:28552200-28552219 MS.gene066758:intron 70.0%
!! CGGGTTCGCGGGTTGGGTCC+AGG - chr2.2:28552202-28552221 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 28551440 28553860 28551440 ID=MS.gene066758
chr2.2 mRNA 28551440 28553860 28551440 ID=MS.gene066758.t1;Parent=MS.gene066758
chr2.2 exon 28551440 28551888 28551440 ID=MS.gene066758.t1.exon1;Parent=MS.gene066758.t1
chr2.2 CDS 28551440 28551888 28551440 ID=cds.MS.gene066758.t1;Parent=MS.gene066758.t1
chr2.2 exon 28553251 28553495 28553251 ID=MS.gene066758.t1.exon2;Parent=MS.gene066758.t1
chr2.2 CDS 28553251 28553495 28553251 ID=cds.MS.gene066758.t1;Parent=MS.gene066758.t1
chr2.2 exon 28553628 28553860 28553628 ID=MS.gene066758.t1.exon3;Parent=MS.gene066758.t1
chr2.2 CDS 28553628 28553860 28553628 ID=cds.MS.gene066758.t1;Parent=MS.gene066758.t1
Gene Sequence

>MS.gene066758

ATGTTGGCTACTGATTCTACAAGAAAGATGTCATTTAATAGAACAATTAGTAACGGTGATTTGGCAATTGTTTATGCAAGGCATGACAACATGAAGCCAGTAACGGTGTCTGAGGGATCGGTTTTACAGAATCGATTCGGTGTTTTCAAACATTCAGATTGGATAGGAAAGTCATTTGGGTCTAAAGTATTCAGCAATAAGGGCGGTTTTGTTTACTTATTAGCACCTACTCCTGAATTGTGGACTTTAGTTTTAAGCCACAGGACACAGATTCTATATGTTGCAGATATCAGCTTTGTTGTTATGTATTTGGAGATTGTTCCTGGTTGTGTCGTTCTTGAATCTGGTACTGGAAGTGGGTCTTTAACTACTTCACTTGCAAGGGCAGTTGCTCCTACAGGACATGTTTATACGTTTGATTTTCACGAACAAAGATCTGGATCAGCTAGGTAATACTGCACTTTTCTTTTTCTGACGATGTTTGATATACATGTGCTTATCTTTAGGCAATGTTGTATGTGTTATTTTCTAATGTTGTCAAATATTGGCGCTATAGCATAGCGGATTTTGATGAATCCGCTAAAAAAAACTGCAACACTATCATAGTTTTGCCGTTATTCTAGTTTTTTGGTAGTTGATGTAGCAACAATAGCAGAAAAAAGAGACTTTTAGGCTGTGTTGTCAATTGTGGAAGGCTAAAAATCTGTCATATAAACACGCCATTACCGCCTAAAAAACGTATTTTTGGTGAAACTGGGTCCTGGACCCAACCCGCGAACCCGGAATTTCAAGGAAGATAACCTGAATCAATGAATTGCAAGATTCCGCCGTACCTACAATTTTCTGCGGTGATGCTGGCTGCACCCACCAAATTCTGATTCCGACCATACTCCAGCATGCCAAGGGGTAGCAAAATCATGCGATCCCATGAGTTTGATCGTGATTTTGACTTCCTTGGCCATGGCAGGCATCCCTTCATAAATTGGCTATGGCGGGCGTAAAAAAATCTGCCTCACAATTCCACCGTGGCCACCATTTGTGAAGGGCATGTTTATACATTTGATTTCCATGAGCATAGGGCTGGATGAGCTATGCAATACACCAGACTTCCTCTGATGTTTGATACATGTGTGTTTATCTTTAGCAAATGTCGAGTATGTTTTTTTCAATGTTGTCAAATTGATTCAATAGCGGCACTATTGTGCTATCACATGGTGGATTTTGATGATCCCCTATGAAAACCGCGGCGCTATCCCAGTTTCGCTATCAGGGCAATAGCGGCAGCTATTAAAGTTTTGCTAGCTGATATACGAGCAATAGTGTAATTTTTTTTCATTTCTTTTAAATAATAAAATTGAAGAATCTGCATGTGTGCCCAACGTGGCTTATTTTTACAATGGAAAAACTTAGGAGTCGGTGAGTATTACTCCTGTATTCACCGTAATACAATATATAAGCTATGCTCTGCCTCCATTCCTTTTTTTATTTGTATGTAGGGTGCCTTGTTTTATATATAATTGACTTATTTCAACTGTTATGCGGTTTCTGCTATTTGCCCGTGTTACTATCTTCTATTTCACGTAGCGGTTTTCCGTAATATGCTATTGATGGCACTTTCATGGCTTTGCATTATATTGTGTTCTCTTTTTTCCAGTAATTGCAAGTAATATTATTGGTGATCTAATACGCTACTGTTCAGAATGTCAACGCTCAAGTTCAAATTTATTTAACAGTACTCAATGCGTTGATAAACTTGAGATGTTCAGTGTAAAGGTAAATTACAGATATAAATATTGTTTTTCTTCTTGTAGGGATGACTTTGAGAAGATTGGCATAAGCAGCTTAGTCACTGCTGGAGTGAGGGACATTCAAGGTGAGGGGTTTCCTGAAGAATTTGCTGGCTTGGCTGATGCAGTTTTTTTGGATCTACCTCAACCTTGGCTAGCTATTCCTTCAGCTGCAAAAATGTTACGACATGACGGCACTTTATGTTCTTTCTCCCCGTGTATTGAACAAGTACAACGATCATGTGAAACAATGCAATCCAACTTCACCGGTACTTCCCCTTTTTTCTTCTTTTATATGTTTCTTATTTTCGCGCTCTCTCACACGTAGTAGTTATGCAACTAATGGTAATTTTATTTAAGCACTGACAAAACTCCTTTGTATCCGTTTGTTTATGTGAAAGATATAAGGACTTTTGAGGTACTCCTACGGACATACGAAGTTCGAGAAGGGAAAATGGAAAGCTTAGAGATTAACGGTGATGACGGCTCTAATGGTTCTCTTCCTTGCAAGAGGAGGCAGCGTTCGGATGGAGCTAGTGTGGTTACCTATCCTAATTCTTCTTCTGTTATGGCTAGACCATGTGGTGAAGCTAGAGGTCACACTGGTTATTTGACATTTGCAAGACTTAATTAA
Protein sequence

>MS.gene066758.t1

MLATDSTRKMSFNRTISNGDLAIVYARHDNMKPVTVSEGSVLQNRFGVFKHSDWIGKSFGSKVFSNKGGFVYLLAPTPELWTLVLSHRTQILYVADISFVVMYLEIVPGCVVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRSGSARDDFEKIGISSLVTAGVRDIQGEGFPEEFAGLADAVFLDLPQPWLAIPSAAKMLRHDGTLCSFSPCIEQVQRSCETMQSNFTDIRTFEVLLRTYEVREGKMESLEINGDDGSNGSLPCKRRQRSDGASVVTYPNSSSVMARPCGEARGHTGYLTFARLN