AlfalfaGEDB | MS.gene067310

M. sativa cultivar XinJiangDaYe / MS.gene067310

KEGG: -
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067310.t1	XP_003627497.1	98.1	108	1	1	1	107	1	108	2.90E-55	224.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067310.t1	Q9ZUZ3	59.0	122	30	3	1	105	1	119	2.5e-31	136.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067310.t1	B7FME9	98.1	108	1	1	1	107	1	108	2.1e-55	224.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene067310	0.835619	1.51E-56	-1.69E-46
MS.gene049669	MS.gene067310	0.805325	1.41E-49	-1.69E-46
MS.gene050750	MS.gene067310	0.841915	3.56E-58	-1.69E-46
MS.gene050953	MS.gene067310	0.832753	7.89E-56	-1.69E-46
MS.gene051064	MS.gene067310	0.850507	1.62E-60	-1.69E-46
MS.gene051368	MS.gene067310	0.809878	1.52E-50	-1.69E-46
MS.gene052176	MS.gene067310	0.807748	4.34E-50	-1.69E-46
MS.gene052333	MS.gene067310	0.86276	4.03E-64	-1.69E-46
MS.gene052353	MS.gene067310	0.80323	3.85E-49	-1.69E-46
MS.gene052389	MS.gene067310	0.859576	3.76E-63	-1.69E-46
MS.gene052586	MS.gene067310	0.815077	1.11E-51	-1.69E-46
MS.gene052590	MS.gene067310	0.812888	3.38E-51	-1.69E-46
MS.gene054291	MS.gene067310	-0.814852	1.25E-51	-1.69E-46
MS.gene054405	MS.gene067310	0.8299	3.97E-55	-1.69E-46
MS.gene054468	MS.gene067310	0.808907	2.46E-50	-1.69E-46
MS.gene055225	MS.gene067310	0.823028	1.73E-53	-1.69E-46
MS.gene055367	MS.gene067310	0.822982	1.77E-53	-1.69E-46
MS.gene055701	MS.gene067310	0.865051	7.81E-65	-1.69E-46
MS.gene05595	MS.gene067310	0.818644	1.76E-52	-1.69E-46
MS.gene057026	MS.gene067310	0.801212	1.00E-48	-1.69E-46
MS.gene057156	MS.gene067310	-0.852253	5.21E-61	-1.69E-46
MS.gene058220	MS.gene067310	0.807128	5.88E-50	-1.69E-46
MS.gene059958	MS.gene067310	0.812421	4.28E-51	-1.69E-46
MS.gene060879	MS.gene067310	0.850427	1.71E-60	-1.69E-46
MS.gene061114	MS.gene067310	-0.854675	1.05E-61	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067310.t1	MTR_8g026730	98.148	108	1	1	1	107	1	108	5.12e-73	212
MS.gene067310.t1	MTR_7g118010	72.477	109	24	2	1	107	1	105	3.42e-49	152
MS.gene067310.t1	MTR_3g092220	62.000	100	34	2	10	105	13	112	2.64e-37	122
MS.gene067310.t1	MTR_0388s0020	55.882	68	29	1	26	92	74	141	7.35e-20	79.0
MS.gene067310.t1	MTR_4g025830	53.333	75	34	1	27	100	79	153	1.49e-19	78.6
MS.gene067310.t1	MTR_4g072300	48.529	68	32	2	27	93	26	91	7.04e-18	72.4
MS.gene067310.t1	MTR_4g072640	44.737	76	39	2	19	93	17	90	2.01e-17	71.2
MS.gene067310.t1	MTR_2g043960	44.156	77	41	1	10	86	59	133	2.42e-17	72.8
MS.gene067310.t1	MTR_5g008370	38.889	90	52	2	8	96	6	93	6.48e-17	71.2
MS.gene067310.t1	MTR_4g072500	48.529	68	32	2	27	93	25	90	1.16e-16	69.3
MS.gene067310.t1	MTR_4g072330	40.000	80	45	2	15	93	11	88	1.68e-16	68.9
MS.gene067310.t1	MTR_4g072220	50.794	63	28	2	27	88	26	86	1.94e-16	68.6
MS.gene067310.t1	MTR_4g072240	45.588	68	34	2	27	93	26	91	2.97e-16	68.2
MS.gene067310.t1	MTR_4g124750	40.260	77	44	1	27	103	69	143	3.43e-16	69.7
MS.gene067310.t1	MTR_1g009150	45.588	68	35	1	26	93	70	135	3.87e-16	70.1
MS.gene067310.t1	MTR_4g072530	50.000	68	31	2	27	93	25	90	4.06e-16	67.8
MS.gene067310.t1	MTR_1g063290	37.895	95	52	1	1	88	6	100	6.14e-16	67.8
MS.gene067310.t1	MTR_4g005320	45.455	77	37	2	27	100	70	144	6.35e-16	69.3
MS.gene067310.t1	MTR_4g072570	45.588	68	34	2	27	93	25	90	8.75e-16	67.0
MS.gene067310.t1	MTR_1g063300	38.542	96	49	2	3	88	5	100	1.13e-15	67.0
MS.gene067310.t1	MTR_4g057810	38.095	84	52	0	5	88	16	99	1.52e-15	67.4
MS.gene067310.t1	MTR_4g072940	49.206	63	29	2	27	88	57	117	2.30e-15	67.0
MS.gene067310.t1	MTR_4g072600	42.500	80	41	3	18	94	9	86	3.57e-15	65.5
MS.gene067310.t1	MTR_3g117640	50.000	66	31	2	26	90	33	97	5.00e-15	65.5
MS.gene067310.t1	MTR_3g109160	39.024	82	48	1	26	107	68	147	7.07e-15	66.6
MS.gene067310.t1	MTR_4g072250	44.118	68	34	2	27	93	26	90	7.43e-15	64.7
MS.gene067310.t1	MTR_3g117610	44.118	68	36	1	27	94	45	110	9.63e-15	64.7
MS.gene067310.t1	MTR_7g101890	43.548	62	35	0	27	88	52	113	1.05e-14	65.1
MS.gene067310.t1	MTR_4g072860	45.588	68	34	2	27	93	26	91	1.06e-14	64.3
MS.gene067310.t1	MTR_4g072980	43.902	82	36	3	18	90	10	90	1.15e-14	64.3
MS.gene067310.t1	MTR_4g072890	44.118	68	35	2	27	93	26	91	1.21e-14	63.9
MS.gene067310.t1	MTR_2g066830	50.000	62	29	1	27	88	58	117	1.36e-14	65.5
MS.gene067310.t1	MTR_3g084250	51.667	60	26	2	27	85	25	82	1.40e-14	63.9
MS.gene067310.t1	MTR_1g063260	35.052	97	54	1	1	88	6	102	1.85e-14	63.9
MS.gene067310.t1	MTR_8g094980	36.585	82	41	1	27	97	3	84	2.15e-14	63.9
MS.gene067310.t1	MTR_4g072880	47.619	63	30	2	27	88	26	86	3.12e-14	63.2
MS.gene067310.t1	MTR_4g072850	47.619	63	30	2	27	88	27	87	3.93e-14	62.8
MS.gene067310.t1	MTR_4g072230	46.032	63	29	2	27	88	26	84	4.12e-14	62.8
MS.gene067310.t1	MTR_1g024085	47.826	69	34	1	27	95	37	103	4.29e-14	63.9
MS.gene067310.t1	MTR_1g059770	40.506	79	43	2	20	96	40	116	4.29e-14	63.5
MS.gene067310.t1	MTR_4g072910	35.106	94	58	2	1	93	9	100	4.33e-14	62.8
MS.gene067310.t1	MTR_4g072870	48.333	60	28	2	27	85	26	83	6.49e-14	62.4
MS.gene067310.t1	MTR_4g072580	40.000	80	43	3	18	94	9	86	7.31e-14	62.0
MS.gene067310.t1	MTR_3g097120	40.000	75	39	1	27	95	9	83	7.44e-14	62.4
MS.gene067310.t1	MTR_4g072310	42.647	68	36	2	27	93	26	91	1.05e-13	61.6
MS.gene067310.t1	MTR_4g072370	36.585	82	49	2	13	93	9	88	1.16e-13	61.6
MS.gene067310.t1	MTR_3g084240	41.772	79	41	3	10	85	6	82	1.19e-13	61.6
MS.gene067310.t1	MTR_4g072480	45.588	68	34	2	27	93	25	90	1.23e-13	61.6
MS.gene067310.t1	MTR_7g101920	40.625	64	38	0	25	88	33	96	1.31e-13	62.0
MS.gene067310.t1	MTR_4g072820	48.333	60	28	2	27	85	20	77	1.41e-13	61.2
MS.gene067310.t1	MTR_8g461420	42.029	69	37	2	27	94	25	91	1.84e-13	61.2
MS.gene067310.t1	MTR_4g072730	42.105	76	41	2	19	93	17	90	1.92e-13	61.2
MS.gene067310.t1	MTR_4g050700	45.070	71	36	2	19	88	18	86	2.27e-13	60.8
MS.gene067310.t1	MTR_2g012410	45.070	71	36	2	19	88	18	86	2.27e-13	60.8
MS.gene067310.t1	MTR_3g084190	42.029	69	37	2	27	94	25	91	2.75e-13	60.5
MS.gene067310.t1	MTR_3g084170	37.975	79	46	2	8	85	6	82	3.38e-13	60.5
MS.gene067310.t1	MTR_7g405740	41.250	80	42	2	25	104	65	139	3.52e-13	62.0
MS.gene067310.t1	MTR_3g084150	42.254	71	38	2	19	88	17	85	3.53e-13	60.5
MS.gene067310.t1	MTR_4g072660	45.588	68	34	2	27	93	20	85	3.64e-13	60.1
MS.gene067310.t1	MTR_3g084180	42.254	71	38	2	19	88	17	85	3.65e-13	60.5
MS.gene067310.t1	MTR_3g117630	45.588	68	34	2	19	85	17	82	3.77e-13	60.5
MS.gene067310.t1	MTR_4g072190	42.424	66	36	1	27	92	36	99	4.42e-13	60.5
MS.gene067310.t1	MTR_4g072260	46.667	60	29	2	27	85	26	83	4.54e-13	60.1
MS.gene067310.t1	MTR_1g063280	37.634	93	45	2	2	81	4	96	4.93e-13	60.5
MS.gene067310.t1	MTR_4g072620	44.118	68	35	2	27	93	25	90	4.99e-13	60.1
MS.gene067310.t1	MTR_7g104940	46.429	56	30	0	38	93	59	114	5.14e-13	60.8
MS.gene067310.t1	MTR_8g461410	40.845	71	39	2	19	88	17	85	5.16e-13	60.1
MS.gene067310.t1	MTR_5g008360	38.158	76	44	2	19	93	8	81	6.06e-13	59.7
MS.gene067310.t1	MTR_4g072610	36.782	87	50	3	11	94	1	85	6.45e-13	59.7
MS.gene067310.t1	MTR_4g072670	42.647	68	36	2	27	93	21	86	6.73e-13	59.3
MS.gene067310.t1	MTR_3g098970	48.387	62	30	1	27	88	53	112	7.49e-13	60.8
MS.gene067310.t1	MTR_4g072270	41.176	68	37	2	27	93	26	91	8.06e-13	59.3
MS.gene067310.t1	MTR_3g084220	40.541	74	41	2	8	80	6	77	8.68e-13	59.3
MS.gene067310.t1	MTR_3g084230	42.029	69	37	2	27	94	25	91	1.11e-12	58.9
MS.gene067310.t1	MTR_4g072560	42.029	69	38	2	26	93	31	98	1.19e-12	59.3
MS.gene067310.t1	MTR_8g096440	42.029	69	38	1	17	85	35	101	1.24e-12	60.1
MS.gene067310.t1	MTR_4g072540	43.077	65	34	2	27	90	21	83	1.44e-12	58.5
MS.gene067310.t1	MTR_3g110042	41.333	75	41	2	19	92	17	89	1.48e-12	58.9
MS.gene067310.t1	MTR_4g072450	40.580	69	38	2	26	93	19	85	1.58e-12	58.5
MS.gene067310.t1	MTR_4g072770	43.478	69	36	2	27	94	15	81	1.80e-12	58.2
MS.gene067310.t1	MTR_4g072630	46.032	63	31	2	27	88	21	81	2.17e-12	58.2
MS.gene067310.t1	MTR_4g072550	47.619	63	30	2	27	88	36	96	2.18e-12	58.5
MS.gene067310.t1	MTR_4g072160	45.588	68	35	1	27	94	37	102	2.22e-12	59.3
MS.gene067310.t1	MTR_3g084210	42.647	68	36	2	27	93	25	90	2.22e-12	58.2
MS.gene067310.t1	MTR_4g072590	46.667	60	29	2	27	85	20	77	2.28e-12	58.2
MS.gene067310.t1	MTR_4g072720	44.615	65	33	2	27	90	21	83	2.37e-12	58.2
MS.gene067310.t1	MTR_7g104950	42.857	56	32	0	38	93	59	114	2.62e-12	58.9
MS.gene067310.t1	MTR_3g110045	42.029	69	37	2	27	94	84	150	2.71e-12	59.7
MS.gene067310.t1	MTR_4g072680	47.619	63	30	2	27	88	25	85	3.01e-12	58.2
MS.gene067310.t1	MTR_3g084160	42.029	69	37	2	27	94	25	91	3.36e-12	57.8
MS.gene067310.t1	MTR_2g095430	38.824	85	50	2	3	85	21	105	3.47e-12	58.9
MS.gene067310.t1	MTR_3g110038	35.366	82	50	2	8	88	6	85	3.62e-12	57.8
MS.gene067310.t1	MTR_4g072460	47.619	63	30	2	27	88	25	85	3.90e-12	57.8
MS.gene067310.t1	MTR_3g113310	39.394	66	38	1	27	92	42	105	3.96e-12	58.2
MS.gene067310.t1	MTR_4g072830	36.145	83	50	2	13	94	9	89	3.97e-12	57.8
MS.gene067310.t1	MTR_1g063330	35.135	74	48	0	15	88	20	93	4.40e-12	57.8
MS.gene067310.t1	MTR_3g110032	44.118	68	35	2	19	85	18	83	4.50e-12	57.4
MS.gene067310.t1	MTR_4g072740	41.176	68	37	2	27	93	20	85	4.82e-12	57.4
MS.gene067310.t1	MTR_4g072750	51.111	45	20	1	27	71	27	69	5.04e-12	57.4
MS.gene067310.t1	MTR_3g084260	44.444	63	32	2	19	80	18	78	6.23e-12	57.4
MS.gene067310.t1	MTR_4g072510	43.077	65	34	2	27	90	21	83	7.14e-12	57.0
MS.gene067310.t1	MTR_1g061570	45.763	59	28	2	27	85	19	73	7.36e-12	57.8
MS.gene067310.t1	MTR_8g096470	40.299	67	38	1	28	94	47	111	8.63e-12	57.4
MS.gene067310.t1	MTR_4g072470	39.130	69	39	2	26	93	19	85	9.86e-12	56.6
MS.gene067310.t1	MTR_4g072490	41.538	65	35	2	27	90	20	82	1.12e-11	56.2
MS.gene067310.t1	MTR_8g096500	44.068	59	31	1	27	85	49	105	1.53e-11	57.8
MS.gene067310.t1	MTR_8g461210	39.130	69	40	1	27	95	35	101	2.11e-11	56.6
MS.gene067310.t1	MTR_4g072650	33.333	87	53	3	11	94	1	85	2.22e-11	55.5
MS.gene067310.t1	MTR_8g076040	42.424	66	36	1	27	92	41	104	2.44e-11	55.8
MS.gene067310.t1	MTR_4g072690	42.188	64	34	2	26	88	19	80	2.73e-11	55.5
MS.gene067310.t1	MTR_4g072280	54.545	44	18	1	27	70	26	67	3.35e-11	55.1
MS.gene067310.t1	MTR_4g021810	43.836	73	37	3	27	97	26	96	3.88e-11	55.5
MS.gene067310.t1	MTR_1g063950	39.437	71	41	1	27	97	48	116	4.05e-11	55.8
MS.gene067310.t1	MTR_1g063340	33.784	74	49	0	15	88	20	93	4.16e-11	55.5
MS.gene067310.t1	MTR_4g057800	36.250	80	49	2	9	86	20	99	4.82e-11	55.8
MS.gene067310.t1	MTR_7g118000	67.647	34	11	0	55	88	27	60	5.73e-11	55.5
MS.gene067310.t1	MTR_5g008350	48.333	60	26	3	25	81	16	73	6.65e-11	54.3
MS.gene067310.t1	MTR_1g063310	37.097	62	39	0	27	88	26	87	7.30e-11	54.7
MS.gene067310.t1	MTR_6g077850	33.333	69	43	2	27	94	34	100	9.54e-11	54.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067310.t1	AT2G46690	67.000	100	29	2	1	99	1	97	1.86e-40	130
MS.gene067310.t1	AT4G00880	69.231	91	27	1	7	97	9	98	5.10e-38	124
MS.gene067310.t1	AT3G61900	62.745	102	34	2	1	98	1	102	6.46e-36	119
MS.gene067310.t1	AT5G53590	54.167	72	30	1	27	95	46	117	1.70e-20	80.5
MS.gene067310.t1	AT3G60690	52.941	68	31	1	27	93	88	155	1.49e-18	76.3
MS.gene067310.t1	AT4G22620	55.556	63	27	1	27	88	79	141	6.00e-18	74.3
MS.gene067310.t1	AT4G12410	50.725	69	33	1	21	88	70	138	7.12e-18	74.3
MS.gene067310.t1	AT5G20810	45.679	81	41	2	27	106	79	157	2.59e-17	72.8
MS.gene067310.t1	AT4G38850	47.297	74	37	2	21	92	15	88	4.31e-17	70.5
MS.gene067310.t1	AT5G20810	48.649	74	35	2	27	99	79	150	5.92e-17	72.4
MS.gene067310.t1	AT3G43120	48.529	68	33	1	19	86	71	136	8.52e-17	71.6
MS.gene067310.t1	AT2G45210	52.239	67	31	1	27	92	79	145	8.89e-17	71.6
MS.gene067310.t1	AT3G03850	50.769	65	31	1	25	88	23	87	1.48e-16	68.9
MS.gene067310.t1	AT2G21210	47.826	69	35	1	18	85	19	87	1.84e-16	68.9
MS.gene067310.t1	AT2G24400	42.667	75	40	2	23	97	60	131	2.23e-16	70.9
MS.gene067310.t1	AT4G34770	40.000	90	53	1	5	93	13	102	6.25e-16	67.8
MS.gene067310.t1	AT2G21210	47.826	69	35	1	18	85	86	154	9.45e-16	68.9
MS.gene067310.t1	AT4G34780	42.500	80	44	2	26	103	27	106	2.16e-15	66.6
MS.gene067310.t1	AT4G31320	40.278	72	41	1	26	97	79	148	2.84e-15	68.2
MS.gene067310.t1	AT5G18050	46.154	65	33	2	25	88	22	85	3.11e-15	65.5
MS.gene067310.t1	AT4G34790	55.000	60	26	1	27	85	39	98	4.90e-15	65.5
MS.gene067310.t1	AT3G12830	42.857	70	38	1	19	88	42	109	6.42e-15	65.9
MS.gene067310.t1	AT3G03840	38.710	93	56	1	1	92	1	93	6.83e-15	64.7
MS.gene067310.t1	AT5G18020	43.662	71	38	2	25	94	22	91	9.14e-15	64.3
MS.gene067310.t1	AT5G18080	47.692	65	32	2	25	88	22	85	1.36e-14	63.9
MS.gene067310.t1	AT4G38825	43.056	72	39	2	18	88	14	84	2.03e-14	63.5
MS.gene067310.t1	AT4G34750	39.474	76	44	1	19	94	29	102	3.18e-14	64.7
MS.gene067310.t1	AT4G34750	39.474	76	44	1	19	94	29	102	3.18e-14	64.7
MS.gene067310.t1	AT1G56150	43.836	73	34	2	27	94	38	108	3.85e-14	63.5
MS.gene067310.t1	AT4G34800	49.275	69	33	2	19	85	13	81	4.59e-14	62.8
MS.gene067310.t1	AT5G18060	48.387	62	30	2	28	88	25	85	5.23e-14	62.4
MS.gene067310.t1	AT4G38840	53.333	60	26	2	27	85	33	91	6.17e-14	62.4
MS.gene067310.t1	AT5G18030	38.961	77	45	2	13	88	8	83	9.23e-14	61.6
MS.gene067310.t1	AT3G03820	48.529	68	33	2	27	92	27	94	2.22e-13	61.2
MS.gene067310.t1	AT1G16510	44.118	68	36	1	21	88	48	113	3.01e-13	62.0
MS.gene067310.t1	AT3G20220	44.444	63	34	1	26	88	46	107	3.16e-13	61.2
MS.gene067310.t1	AT2G21220	42.424	66	36	1	27	92	40	103	3.60e-13	60.8
MS.gene067310.t1	AT1G79130	43.750	64	34	1	25	88	46	107	4.78e-13	61.2
MS.gene067310.t1	AT2G21200	47.541	61	30	2	26	85	20	79	5.56e-13	59.7
MS.gene067310.t1	AT4G34810	50.000	62	28	2	27	85	48	109	5.72e-13	60.5
MS.gene067310.t1	AT5G66260	40.845	71	40	1	23	93	30	98	9.33e-13	59.7
MS.gene067310.t1	AT4G13790	42.647	68	38	1	26	92	24	91	1.01e-12	59.3
MS.gene067310.t1	AT4G34760	42.424	66	36	1	27	92	43	106	2.08e-12	58.9
MS.gene067310.t1	AT5G18010	43.077	65	35	2	25	88	22	85	2.56e-12	58.2
MS.gene067310.t1	AT2G28085	47.458	59	30	1	31	88	60	118	3.76e-12	58.5
MS.gene067310.t1	AT1G75580	41.791	67	37	1	26	92	43	107	1.23e-11	57.0
MS.gene067310.t1	AT5G10990	41.429	70	39	1	27	96	41	108	1.39e-11	57.8
MS.gene067310.t1	AT1G19840	47.458	59	29	1	27	85	41	97	2.17e-11	57.4
MS.gene067310.t1	AT5G50760	41.463	82	38	3	22	97	44	121	3.84e-11	57.0
MS.gene067310.t1	AT4G38860	41.791	67	36	2	27	92	40	104	5.61e-11	55.1
MS.gene067310.t1	AT3G03830	44.118	68	36	2	27	92	24	91	6.64e-11	54.7

Find 20 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAACATGTTGGTTGTTT+TGG	0.174789	8.4:+70089732	MS.gene067310:CDS
TTTATCGCCATAAAACCTTT+TGG	0.276868	8.4:-70089519	None:intergenic
GAATATGGATTTGATCAAAA+AGG	0.314878	8.4:+70089636	MS.gene067310:CDS
CTATGCAAATGAAGACTCTT+TGG	0.322112	8.4:-70089459	None:intergenic
AAAGAGGCAGAAGAAGAATA+TGG	0.327570	8.4:+70089621	MS.gene067310:CDS
TTCAATAGTTGAATGAAAAG+AGG	0.392056	8.4:-70089600	None:intergenic
ATGGCGATAAAAGTTGGTCT+AGG	0.406481	8.4:+70089531	MS.gene067310:CDS
TTTCATTCAACTATTGAAAG+AGG	0.462076	8.4:+70089605	MS.gene067310:CDS
TGGCGATAAAAGTTGGTCTA+GGG	0.473696	8.4:+70089532	MS.gene067310:CDS
TACTATTCCTTGTCATGTTG+AGG	0.479799	8.4:+70089665	MS.gene067310:CDS
GAGTTCAAAGGTGTTCCAAA+AGG	0.492578	8.4:+70089504	MS.gene067310:CDS
ACAACAAAGGTTTGTTGTGC+CGG	0.497789	8.4:+70089560	MS.gene067310:CDS
TCAATAGTTGAATGAAAAGA+GGG	0.540406	8.4:-70089599	None:intergenic
GAGGAATTTAGAAACGTTCG+TGG	0.549061	8.4:+70089684	MS.gene067310:CDS
CTAAATTCCTCAACATGACA+AGG	0.555962	8.4:-70089672	None:intergenic
CAACATAAGCAAGAGTTCAA+AGG	0.569608	8.4:+70089492	MS.gene067310:CDS
AACATGACAAGGAATAGTAA+TGG	0.599614	8.4:-70089661	None:intergenic
GGCGATAAAAGTTGGTCTAG+GGG	0.616753	8.4:+70089533	MS.gene067310:CDS
GGGTGATTGAAGTACATCAC+CGG	0.643631	8.4:-70089579	None:intergenic
GTCTAGGGGAAGAACAACAA+AGG	0.657155	8.4:+70089547	MS.gene067310:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAATCTTCATCAACATGT+TGG	+	chr8.4:70089723-70089742	MS.gene067310:CDS	25.0%
!	GAATATGGATTTGATCAAAA+AGG	+	chr8.4:70089636-70089655	MS.gene067310:CDS	25.0%
!	TCAATAGTTGAATGAAAAGA+GGG	-	chr8.4:70089602-70089621	None:intergenic	25.0%
!	TTCAATAGTTGAATGAAAAG+AGG	-	chr8.4:70089603-70089622	None:intergenic	25.0%
!	TTTCATTCAACTATTGAAAG+AGG	+	chr8.4:70089605-70089624	MS.gene067310:CDS	25.0%
	AACATGACAAGGAATAGTAA+TGG	-	chr8.4:70089664-70089683	None:intergenic	30.0%
!	TTTATCGCCATAAAACCTTT+TGG	-	chr8.4:70089522-70089541	None:intergenic	30.0%
	AAAGAGGCAGAAGAAGAATA+TGG	+	chr8.4:70089621-70089640	MS.gene067310:CDS	35.0%
	CAACATAAGCAAGAGTTCAA+AGG	+	chr8.4:70089492-70089511	MS.gene067310:CDS	35.0%
	CTAAATTCCTCAACATGACA+AGG	-	chr8.4:70089675-70089694	None:intergenic	35.0%
	CTATGCAAATGAAGACTCTT+TGG	-	chr8.4:70089462-70089481	None:intergenic	35.0%
	TACTATTCCTTGTCATGTTG+AGG	+	chr8.4:70089665-70089684	MS.gene067310:CDS	35.0%
!	AGGTGTTCCAAAAGGTTTTA+TGG	+	chr8.4:70089512-70089531	MS.gene067310:CDS	35.0%
!!	CATCAACATGTTGGTTGTTT+TGG	+	chr8.4:70089732-70089751	MS.gene067310:CDS	35.0%
!!	GGTTTTATGGCGATAAAAGT+TGG	+	chr8.4:70089525-70089544	MS.gene067310:CDS	35.0%
	GAGGAATTTAGAAACGTTCG+TGG	+	chr8.4:70089684-70089703	MS.gene067310:CDS	40.0%
!	ATGGCGATAAAAGTTGGTCT+AGG	+	chr8.4:70089531-70089550	MS.gene067310:CDS	40.0%
!	TGGCGATAAAAGTTGGTCTA+GGG	+	chr8.4:70089532-70089551	MS.gene067310:CDS	40.0%
!!	ACAACAAAGGTTTGTTGTGC+CGG	+	chr8.4:70089560-70089579	MS.gene067310:CDS	40.0%
!!	GAGTTCAAAGGTGTTCCAAA+AGG	+	chr8.4:70089504-70089523	MS.gene067310:CDS	40.0%
	GGGTGATTGAAGTACATCAC+CGG	-	chr8.4:70089582-70089601	None:intergenic	45.0%
	GTCTAGGGGAAGAACAACAA+AGG	+	chr8.4:70089547-70089566	MS.gene067310:CDS	45.0%
!	GGCGATAAAAGTTGGTCTAG+GGG	+	chr8.4:70089533-70089552	MS.gene067310:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	70089438	70089761	70089438	ID=MS.gene067310
chr8.4	mRNA	70089438	70089761	70089438	ID=MS.gene067310.t1;Parent=MS.gene067310
chr8.4	exon	70089438	70089761	70089438	ID=MS.gene067310.t1.exon1;Parent=MS.gene067310.t1
chr8.4	CDS	70089438	70089761	70089438	ID=cds.MS.gene067310.t1;Parent=MS.gene067310.t1

Gene Sequence	>MS.gene067310 ATGGGTTTGGTTGAAAAGAGTCCAAAGAGTCTTCATTTGCATAGACATCATCTTCAACATAAGCAAGAGTTCAAAGGTGTTCCAAAAGGTTTTATGGCGATAAAAGTTGGTCTAGGGGAAGAACAACAAAGGTTTGTTGTGCCGGTGATGTACTTCAATCACCCTCTTTTCATTCAACTATTGAAAGAGGCAGAAGAAGAATATGGATTTGATCAAAAAGGAACCATTACTATTCCTTGTCATGTTGAGGAATTTAGAAACGTTCGTGGTTTGATTGATAGAGACAAAAATCTTCATCAACATGTTGGTTGTTTTGGTTTGTGA
Protein sequence	>MS.gene067310.t1 MGLVEKSPKSLHLHRHHLQHKQEFKGVPKGFMAIKVGLGEEQQRFVVPVMYFNHPLFIQLLKEAEEEYGFDQKGTITIPCHVEEFRNVRGLIDRDKNLHQHVGCFGL