AlfalfaGEDB | MS.gene067656

M. sativa cultivar XinJiangDaYe / MS.gene067656

KEGG: -
GO: GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0032259 GO:0034641 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044728 GO:0046483 GO:0071704 GO:0090304 GO:1901360
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067656.t1	KEH27550.1	93	100	7	0	1	100	429	528	5.80E-45	189.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067656.t1	Q9LXE5	63.5	96	35	0	1	96	524	619	9.3e-28	124.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067656.t1	G7KC46	93.0	100	7	0	1	100	491	590	4.2e-45	189.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050611	MS.gene067656	0.8173	3.54E-52	-1.69E-46
MS.gene051710	MS.gene067656	0.801358	9.35E-49	-1.69E-46
MS.gene052829	MS.gene067656	0.81574	7.92E-52	-1.69E-46
MS.gene052843	MS.gene067656	0.803805	2.93E-49	-1.69E-46
MS.gene052867	MS.gene067656	0.818648	1.76E-52	-1.69E-46
MS.gene053512	MS.gene067656	0.805683	1.19E-49	-1.69E-46
MS.gene053974	MS.gene067656	0.844009	9.86E-59	-1.69E-46
MS.gene054236	MS.gene067656	0.806478	8.07E-50	-1.69E-46
MS.gene058515	MS.gene067656	0.824769	6.75E-54	-1.69E-46
MS.gene059276	MS.gene067656	0.815337	9.73E-52	-1.69E-46
MS.gene059584	MS.gene067656	0.808441	3.09E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067656.t1	MTR_5g019420	93.000	100	7	0	1	100	429	528	4.18e-61	194
MS.gene067656.t1	MTR_5g019420	93.000	100	7	0	1	100	491	590	7.74e-61	195
MS.gene067656.t1	MTR_3g011150	89.000	100	11	0	1	100	491	590	2.60e-60	193
MS.gene067656.t1	MTR_6g005580	79.000	100	21	0	1	100	489	588	6.26e-53	174
MS.gene067656.t1	MTR_4g123600	35.354	99	61	2	1	98	626	722	2.58e-16	72.8

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067656.t1	AT5G15380	63.542	96	35	1	96	524	619	8.71e-36	128
MS.gene067656.t1	AT5G14620	61.702	94	36	1	94	528	621	1.47e-35	127
MS.gene067656.t1	AT3G17310	33.684	95	63	1	95	496	590	1.28e-14	68.2
MS.gene067656.t1	AT3G17310	33.684	95	63	1	95	615	709	1.44e-14	68.2
MS.gene067656.t1	AT3G17310	33.684	95	63	1	95	615	709	1.44e-14	68.2

Find 25 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGGAACAAACTAATCAAAA+AGG	0.357878	4.1:+68877772	MS.gene067656:CDS
ATAACCTGGCTGGAAGCAAC+AGG	0.392905	4.1:+68877902	MS.gene067656:CDS
ACAGCTAGATGCTGAGCGTT+TGG	0.394226	4.1:+68877822	MS.gene067656:CDS
ATAGGAACATTGTTAGAAGT+TGG	0.454909	4.1:+68877749	MS.gene067656:CDS
TTCGAAAGTAATCGCAAAAG+AGG	0.482160	4.1:-68877960	None:intergenic
AGCAACAGGGTTAGACGGAT+TGG	0.486406	4.1:+68877916	MS.gene067656:CDS
CTTCCAGCCAGGTTATTGCA+TGG	0.501006	4.1:-68877895	None:intergenic
TAACCCTGTTGCTTCCAGCC+AGG	0.508180	4.1:-68877906	None:intergenic
AGGGTTAGACGGATTGGACT+CGG	0.508374	4.1:+68877922	MS.gene067656:CDS
TTTAGACTTGGTGAAAGCTA+TGG	0.511549	4.1:+68877984	MS.gene067656:CDS
GGGTTAGACGGATTGGACTC+GGG	0.511894	4.1:+68877923	MS.gene067656:CDS
GTCCCTCTAAATAATGTTGT+CGG	0.517042	4.1:+68877708	None:intergenic
GGAACATTGTTAGAAGTTGG+TGG	0.526472	4.1:+68877752	MS.gene067656:CDS
ATACAATTTCATTGAAACCT+CGG	0.528933	4.1:-68878009	None:intergenic
AGTCCATGCAATAACCTGGC+TGG	0.538292	4.1:+68877892	MS.gene067656:CDS
GGTTAGACGGATTGGACTCG+GGG	0.565804	4.1:+68877924	MS.gene067656:CDS
GAACATTGTTAGAAGTTGGT+GGG	0.581341	4.1:+68877753	MS.gene067656:CDS
CTGGAAGCAACAGGGTTAGA+CGG	0.593430	4.1:+68877911	MS.gene067656:CDS
TGGGAGTCCATGCAATAACC+TGG	0.607993	4.1:+68877888	MS.gene067656:CDS
TCGAAAGTAATCGCAAAAGA+GGG	0.613902	4.1:-68877959	None:intergenic
GAGCAGCTCATGAGTGCATG+TGG	0.636691	4.1:+68877844	MS.gene067656:CDS
CAGCTCATGAGTGCATGTGG+TGG	0.640731	4.1:+68877847	MS.gene067656:CDS
TGGTGAAAGCTATGGCGCCG+AGG	0.661770	4.1:+68877992	MS.gene067656:CDS
TAACCTGGCTGGAAGCAACA+GGG	0.687399	4.1:+68877903	MS.gene067656:CDS
GTTAGACGGATTGGACTCGG+GGG	0.743368	4.1:+68877925	MS.gene067656:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGAAAAATCTAAAGTGAAT+AGG	+	chr4.1:68877731-68877750	MS.gene067656:CDS	20.0%
!!	TTACTTTCGAATTTTAGACT+TGG	+	chr4.1:68877972-68877991	MS.gene067656:CDS	25.0%
	ATAGGAACATTGTTAGAAGT+TGG	+	chr4.1:68877749-68877768	MS.gene067656:CDS	30.0%
	TGGGAACAAACTAATCAAAA+AGG	+	chr4.1:68877772-68877791	MS.gene067656:CDS	30.0%
	TCGAAAGTAATCGCAAAAGA+GGG	-	chr4.1:68877962-68877981	None:intergenic	35.0%
	TTCGAAAGTAATCGCAAAAG+AGG	-	chr4.1:68877963-68877982	None:intergenic	35.0%
!	GAACATTGTTAGAAGTTGGT+GGG	+	chr4.1:68877753-68877772	MS.gene067656:CDS	35.0%
!!	TTTAGACTTGGTGAAAGCTA+TGG	+	chr4.1:68877984-68878003	MS.gene067656:CDS	35.0%
	GGAACATTGTTAGAAGTTGG+TGG	+	chr4.1:68877752-68877771	MS.gene067656:CDS	40.0%
!!	GTTTTGATCTCGTTGTTGGT+GGG	+	chr4.1:68877869-68877888	MS.gene067656:CDS	40.0%
!!	GGTTTTGATCTCGTTGTTGG+TGG	+	chr4.1:68877868-68877887	MS.gene067656:CDS	45.0%
!!!	GGTGGTTTTGATCTCGTTGT+TGG	+	chr4.1:68877865-68877884	MS.gene067656:CDS	45.0%
	ACAGCTAGATGCTGAGCGTT+TGG	+	chr4.1:68877822-68877841	MS.gene067656:CDS	50.0%
	AGCAACAGGGTTAGACGGAT+TGG	+	chr4.1:68877916-68877935	MS.gene067656:CDS	50.0%
	AGTCCATGCAATAACCTGGC+TGG	+	chr4.1:68877892-68877911	MS.gene067656:CDS	50.0%
	ATAACCTGGCTGGAAGCAAC+AGG	+	chr4.1:68877902-68877921	MS.gene067656:CDS	50.0%
	CTGGAAGCAACAGGGTTAGA+CGG	+	chr4.1:68877911-68877930	MS.gene067656:CDS	50.0%
	CTTCCAGCCAGGTTATTGCA+TGG	-	chr4.1:68877898-68877917	None:intergenic	50.0%
	TAACCTGGCTGGAAGCAACA+GGG	+	chr4.1:68877903-68877922	MS.gene067656:CDS	50.0%
	TGGGAGTCCATGCAATAACC+TGG	+	chr4.1:68877888-68877907	MS.gene067656:CDS	50.0%
!!	AGGGTTAGACGGATTGGACT+CGG	+	chr4.1:68877922-68877941	MS.gene067656:CDS	50.0%
	CAGCTCATGAGTGCATGTGG+TGG	+	chr4.1:68877847-68877866	MS.gene067656:CDS	55.0%
	GAGCAGCTCATGAGTGCATG+TGG	+	chr4.1:68877844-68877863	MS.gene067656:CDS	55.0%
!	TAACCCTGTTGCTTCCAGCC+AGG	-	chr4.1:68877909-68877928	None:intergenic	55.0%
!!	GGGTTAGACGGATTGGACTC+GGG	+	chr4.1:68877923-68877942	MS.gene067656:CDS	55.0%
!!	GGTTAGACGGATTGGACTCG+GGG	+	chr4.1:68877924-68877943	MS.gene067656:CDS	55.0%
!!	GTTAGACGGATTGGACTCGG+GGG	+	chr4.1:68877925-68877944	MS.gene067656:CDS	55.0%
	TGGTGAAAGCTATGGCGCCG+AGG	+	chr4.1:68877992-68878011	MS.gene067656:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	68877721	68878023	68877721	ID=MS.gene067656
chr4.1	mRNA	68877721	68878023	68877721	ID=MS.gene067656.t1;Parent=MS.gene067656
chr4.1	exon	68877721	68878023	68877721	ID=MS.gene067656.t1.exon1;Parent=MS.gene067656.t1
chr4.1	CDS	68877721	68878023	68877721	ID=cds.MS.gene067656.t1;Parent=MS.gene067656.t1

Gene Sequence	>MS.gene067656 ATGTTGTCGGTTGAAAAATCTAAAGTGAATAGGAACATTGTTAGAAGTTGGTGGGAACAAACTAATCAAAAAGGTAACTTGATTGAAATCGATGATGTGCAACAGCTAGATGCTGAGCGTTTGGAGCAGCTCATGAGTGCATGTGGTGGTTTTGATCTCGTTGTTGGTGGGAGTCCATGCAATAACCTGGCTGGAAGCAACAGGGTTAGACGGATTGGACTCGGGGGAACAGAGTCAGCCCTCTTTTGCGATTACTTTCGAATTTTAGACTTGGTGAAAGCTATGGCGCCGAGGTTTCAATGA
Protein sequence	>MS.gene067656.t1 MLSVEKSKVNRNIVRSWWEQTNQKGNLIEIDDVQQLDAERLEQLMSACGGFDLVVGGSPCNNLAGSNRVRRIGLGGTESALFCDYFRILDLVKAMAPRFQ