Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052519 | MS.gene067898 | 0.852772 | 3.71E-61 | -1.69E-46 |
| MS.gene052863 | MS.gene067898 | 0.809806 | 1.58E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene067898 | 0.803318 | 3.69E-49 | -1.69E-46 |
| MS.gene053145 | MS.gene067898 | 0.803414 | 3.53E-49 | -1.69E-46 |
| MS.gene053146 | MS.gene067898 | 0.81021 | 1.29E-50 | -1.69E-46 |
| MS.gene053900 | MS.gene067898 | 0.80755 | 4.78E-50 | -1.69E-46 |
| MS.gene054582 | MS.gene067898 | 0.815378 | 9.53E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene067898 | 0.818288 | 2.12E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene067898 | 0.831661 | 1.47E-55 | -1.69E-46 |
| MS.gene058009 | MS.gene067898 | 0.810568 | 1.08E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene067898 | 0.810352 | 1.20E-50 | -1.69E-46 |
| MS.gene058783 | MS.gene067898 | 0.830781 | 2.42E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGTCTTTGGGGTTCTATT+CGG | 0.240004 | 8.2:+31978890 | MS.gene067898:CDS |
| GGTGGCCTGCTATTGTCTTT+GGG | 0.240339 | 8.2:+31978879 | MS.gene067898:CDS |
| TGGTGGCCTGCTATTGTCTT+TGG | 0.250761 | 8.2:+31978878 | MS.gene067898:CDS |
| TGTGGCATAGCAGGGTTCTT+TGG | 0.318245 | 8.2:+31978921 | MS.gene067898:CDS |
| TTGTCTTTGGGGTTCTATTC+GGG | 0.328544 | 8.2:+31978891 | MS.gene067898:CDS |
| GGTTAAGAGAAGAATTAGTT+TGG | 0.388719 | 8.2:+31978803 | MS.gene067898:intron |
| GGTGTTGACTGTGGCATAGC+AGG | 0.394517 | 8.2:+31978912 | MS.gene067898:CDS |
| TGCTGGGAGCTGGCTGCAGC+GGG | 0.406548 | 8.2:+31979040 | MS.gene067898:CDS |
| GTGGCCTGCTATTGTCTTTG+GGG | 0.438758 | 8.2:+31978880 | MS.gene067898:CDS |
| GTGAGGTGAATGGTGATTTG+AGG | 0.461694 | 8.2:+31978832 | MS.gene067898:CDS |
| TTATAGCCGTGGTGGCTTTG+TGG | 0.474791 | 8.2:+31979063 | MS.gene067898:CDS |
| TTGCTGGGAGCTGGCTGCAG+CGG | 0.522395 | 8.2:+31979039 | MS.gene067898:CDS |
| TTGGCCTCTTGTGAGGTGAA+TGG | 0.526844 | 8.2:+31978822 | MS.gene067898:CDS |
| AGAACCCCAAAGACAATAGC+AGG | 0.529164 | 8.2:-31978884 | None:intergenic |
| ATGCTTCTTATGAGCTGCAA+AGG | 0.533894 | 8.2:+31977896 | MS.gene067898:CDS |
| AATTAGTTTGGCCTCTTGTG+AGG | 0.555483 | 8.2:+31978815 | MS.gene067898:CDS |
| TGCACAATGGATGATAATCA+AGG | 0.575904 | 8.2:+31977498 | None:intergenic |
| GCTGCAGCGGGTTATAGCCG+TGG | 0.578545 | 8.2:+31979052 | MS.gene067898:CDS |
| GTGTTGACTGTGGCATAGCA+GGG | 0.585224 | 8.2:+31978913 | MS.gene067898:CDS |
| TTCTATTCGGGTGTTGACTG+TGG | 0.585419 | 8.2:+31978903 | MS.gene067898:CDS |
| ATCACCATTCACCTCACAAG+AGG | 0.594909 | 8.2:-31978826 | None:intergenic |
| CGATGAGCAGCAGAAAGAGA+AGG | 0.612760 | 8.2:+31977924 | MS.gene067898:CDS |
| GCAGCGGGTTATAGCCGTGG+TGG | 0.619905 | 8.2:+31979055 | MS.gene067898:CDS |
| GACTAGCCACAAAGCCACCA+CGG | 0.651795 | 8.2:-31979069 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAAAAACTTAAAAAAT+AGG | + | chr8.2:31978478-31978497 | MS.gene067898:intron | 10.0% |
| !! | AATATATAATAAAACCTAAA+AGG | - | chr8.2:31978577-31978596 | None:intergenic | 10.0% |
| !!! | ATTTTTTTTTTATATGTAGA+AGG | + | chr8.2:31978964-31978983 | MS.gene067898:intron | 10.0% |
| !!! | TTTTAGGTTTTATTATATAT+TGG | + | chr8.2:31978576-31978595 | MS.gene067898:intron | 10.0% |
| !!! | ATAGTAATTAATTTGAAGTA+TGG | - | chr8.2:31978731-31978750 | None:intergenic | 15.0% |
| !! | AACTCTTATAATACTTATTC+AGG | + | chr8.2:31977865-31977884 | MS.gene067898:intron | 20.0% |
| !! | AATTGAAATTATGAAACATG+AGG | - | chr8.2:31978378-31978397 | None:intergenic | 20.0% |
| !! | ACTCTTATAATACTTATTCA+GGG | + | chr8.2:31977866-31977885 | MS.gene067898:intron | 20.0% |
| !! | CTGTTAGATAATAAGTTATT+AGG | + | chr8.2:31978510-31978529 | MS.gene067898:intron | 20.0% |
| !! | TATCATAACTCATTAAACTA+AGG | - | chr8.2:31978610-31978629 | None:intergenic | 20.0% |
| !! | TCATGTTTCATAATTTCAAT+TGG | + | chr8.2:31978377-31978396 | MS.gene067898:intron | 20.0% |
| !!! | TAAGTTGGATAATTATTTGA+TGG | + | chr8.2:31977525-31977544 | MS.gene067898:intron | 20.0% |
| ! | AGCCATAAACATGATTAAAT+CGG | - | chr8.2:31978770-31978789 | None:intergenic | 25.0% |
| ! | AGGATAGAATGGTATTAAAA+GGG | + | chr8.2:31977605-31977624 | MS.gene067898:intron | 25.0% |
| ! | ATAGTTATTCTATTGAAACC+TGG | + | chr8.2:31978629-31978648 | MS.gene067898:intron | 25.0% |
| ! | CTCTTATAATACTTATTCAG+GGG | + | chr8.2:31977867-31977886 | MS.gene067898:intron | 25.0% |
| ! | TCATCTTATAAACATGTAGT+AGG | + | chr8.2:31977977-31977996 | MS.gene067898:intron | 25.0% |
| ! | TTCCGATTTAATCATGTTTA+TGG | + | chr8.2:31978765-31978784 | MS.gene067898:intron | 25.0% |
| !! | CTTTGAAAATGAAATTAGCA+AGG | - | chr8.2:31977733-31977752 | None:intergenic | 25.0% |
| !!! | TTCATTGATTTTTCCTCATT+TGG | - | chr8.2:31978352-31978371 | None:intergenic | 25.0% |
| AAATGAAATTAGCAAGGCAA+AGG | - | chr8.2:31977727-31977746 | None:intergenic | 30.0% | |
| AATTTCTTCTGTGGAATCAA+TGG | + | chr8.2:31978129-31978148 | MS.gene067898:intron | 30.0% | |
| ACATGAAATTAGTAAAGCAG+AGG | - | chr8.2:31977809-31977828 | None:intergenic | 30.0% | |
| AGAGAACAAAACAAAAACGA+CGG | - | chr8.2:31978655-31978674 | None:intergenic | 30.0% | |
| CAAGAATGAAATGCAAACTA+TGG | + | chr8.2:31977642-31977661 | MS.gene067898:intron | 30.0% | |
| CTAATTTCATGTCCTGTATT+CGG | + | chr8.2:31977817-31977836 | MS.gene067898:intron | 30.0% | |
| GAGGATAGAATGGTATTAAA+AGG | + | chr8.2:31977604-31977623 | MS.gene067898:intron | 30.0% | |
| GGTTAAGAGAAGAATTAGTT+TGG | + | chr8.2:31978803-31978822 | MS.gene067898:intron | 30.0% | |
| TAATTTCATGTCCTGTATTC+GGG | + | chr8.2:31977818-31977837 | MS.gene067898:intron | 30.0% | |
| TGATGGAAAGAATCCTAAAT+CGG | + | chr8.2:31977953-31977972 | MS.gene067898:intron | 30.0% | |
| TTCTCTTAACCTAAAAGAAG+AGG | - | chr8.2:31978794-31978813 | None:intergenic | 30.0% | |
| TTGATTCCACAGAAGAAATT+GGG | - | chr8.2:31978129-31978148 | None:intergenic | 30.0% | |
| ! | AGAAGACTTCAACTCTAAAA+TGG | - | chr8.2:31978409-31978428 | None:intergenic | 30.0% |
| ! | ATTGATTCCACAGAAGAAAT+TGG | - | chr8.2:31978130-31978149 | None:intergenic | 30.0% |
| ! | ATTTTTCCTCATTTGGTTAG+TGG | - | chr8.2:31978345-31978364 | None:intergenic | 30.0% |
| ! | TAAGACAAAGTTCACTCTTT+TGG | - | chr8.2:31978293-31978312 | None:intergenic | 30.0% |
| ! | TACTTCAAACTTTGAAGATG+AGG | - | chr8.2:31978056-31978075 | None:intergenic | 30.0% |
| ! | TTTATAAGATGAGCCGATTT+AGG | - | chr8.2:31977969-31977988 | None:intergenic | 30.0% |
| ! | TTTTTCCTCATTTGGTTAGT+GGG | - | chr8.2:31978344-31978363 | None:intergenic | 30.0% |
| !!! | GTTTTTTGAATTCTGTTGCA+TGG | + | chr8.2:31978990-31979009 | MS.gene067898:CDS | 30.0% |
| AATTTCATGTCCTGTATTCG+GGG | + | chr8.2:31977819-31977838 | MS.gene067898:intron | 35.0% | |
| ACAATACATAGAGCTAGAGA+CGG | - | chr8.2:31978685-31978704 | None:intergenic | 35.0% | |
| ACCAAACTTCATCATGCAAT+AGG | - | chr8.2:31978251-31978270 | None:intergenic | 35.0% | |
| AGTTATTAGGCTCATGTTGT+TGG | + | chr8.2:31978523-31978542 | MS.gene067898:intron | 35.0% | |
| CAAGTCTATCAATTCTCTAG+AGG | + | chr8.2:31977585-31977604 | MS.gene067898:intron | 35.0% | |
| GAAAGAGAAGGTAGAATTGA+TGG | + | chr8.2:31977936-31977955 | MS.gene067898:intron | 35.0% | |
| GAATGAAATGCAAACTATGG+AGG | + | chr8.2:31977645-31977664 | MS.gene067898:intron | 35.0% | |
| GTTATTAGGCTCATGTTGTT+GGG | + | chr8.2:31978524-31978543 | MS.gene067898:intron | 35.0% | |
| TCCTATTGCATGATGAAGTT+TGG | + | chr8.2:31978247-31978266 | MS.gene067898:intron | 35.0% | |
| TGATTCCACAGAAGAAATTG+GGG | - | chr8.2:31978128-31978147 | None:intergenic | 35.0% | |
| TTCTATAACAGCACTGTATC+TGG | + | chr8.2:31978021-31978040 | MS.gene067898:intron | 35.0% | |
| ! | CAATTCTCTAGAGGATAGAA+TGG | + | chr8.2:31977594-31977613 | MS.gene067898:intron | 35.0% |
| ! | GAAGTTAGTAGACTCCTTTT+AGG | + | chr8.2:31978560-31978579 | MS.gene067898:intron | 35.0% |
| ! | TTATGGCTTCCTCTTCTTTT+AGG | + | chr8.2:31978782-31978801 | MS.gene067898:intron | 35.0% |
| !! | ATTGTCTTTGGGGTTCTATT+CGG | + | chr8.2:31978890-31978909 | MS.gene067898:CDS | 35.0% |
| !! | TTGAGGCTTGTTCTTTTACT+TGG | + | chr8.2:31978849-31978868 | MS.gene067898:CDS | 35.0% |
| !!! | ACTTTTCTGGTTTTGTTGCT+GGG | + | chr8.2:31979024-31979043 | MS.gene067898:CDS | 35.0% |
| ATGCTTCTTATGAGCTGCAA+AGG | + | chr8.2:31977896-31977915 | MS.gene067898:CDS | 40.0% | |
| CAAACTAATACCCCGAATAC+AGG | - | chr8.2:31977832-31977851 | None:intergenic | 40.0% | |
| GATAATCAAGGTGGCTAAGT+TGG | + | chr8.2:31977510-31977529 | MS.gene067898:intron | 40.0% | |
| ! | AAACTTTGAAGATGAGGCAC+AGG | - | chr8.2:31978050-31978069 | None:intergenic | 40.0% |
| ! | AATTAGTTTGGCCTCTTGTG+AGG | + | chr8.2:31978815-31978834 | MS.gene067898:CDS | 40.0% |
| ! | GCTTGTTCTTTTACTTGGAG+GGG | + | chr8.2:31978854-31978873 | MS.gene067898:CDS | 40.0% |
| !! | AGGCTTGTTCTTTTACTTGG+AGG | + | chr8.2:31978852-31978871 | MS.gene067898:CDS | 40.0% |
| !! | GGCTTGTTCTTTTACTTGGA+GGG | + | chr8.2:31978853-31978872 | MS.gene067898:CDS | 40.0% |
| !! | GGTCTTCTGATTCACTTTTC+TGG | + | chr8.2:31979011-31979030 | MS.gene067898:CDS | 40.0% |
| !! | TTGTCTTTGGGGTTCTATTC+GGG | + | chr8.2:31978891-31978910 | MS.gene067898:CDS | 40.0% |
| !!! | CACTTTTCTGGTTTTGTTGC+TGG | + | chr8.2:31979023-31979042 | MS.gene067898:CDS | 40.0% |
| ACAAAACAAAAACGACGGCC+AGG | - | chr8.2:31978650-31978669 | None:intergenic | 45.0% | |
| AGAACCCCAAAGACAATAGC+AGG | - | chr8.2:31978887-31978906 | None:intergenic | 45.0% | |
| ATCACCATTCACCTCACAAG+AGG | - | chr8.2:31978829-31978848 | None:intergenic | 45.0% | |
| CTCATTTGGTTAGTGGGAGA+AGG | - | chr8.2:31978338-31978357 | None:intergenic | 45.0% | |
| CTTCTCCCACTAACCAAATG+AGG | + | chr8.2:31978336-31978355 | MS.gene067898:intron | 45.0% | |
| GTGAGGTGAATGGTGATTTG+AGG | + | chr8.2:31978832-31978851 | MS.gene067898:CDS | 45.0% | |
| TTCTGCCCCAATTTCTTCTG+TGG | + | chr8.2:31978120-31978139 | MS.gene067898:intron | 45.0% | |
| ! | GTTCTTTTACTTGGAGGGGT+TGG | + | chr8.2:31978858-31978877 | MS.gene067898:CDS | 45.0% |
| !! | TTCTATTCGGGTGTTGACTG+TGG | + | chr8.2:31978903-31978922 | MS.gene067898:CDS | 45.0% |
| CGATGAGCAGCAGAAAGAGA+AGG | + | chr8.2:31977924-31977943 | MS.gene067898:CDS | 50.0% | |
| GGTGGCCTGCTATTGTCTTT+GGG | + | chr8.2:31978879-31978898 | MS.gene067898:CDS | 50.0% | |
| GTGGCCTGCTATTGTCTTTG+GGG | + | chr8.2:31978880-31978899 | MS.gene067898:CDS | 50.0% | |
| TGGTGGCCTGCTATTGTCTT+TGG | + | chr8.2:31978878-31978897 | MS.gene067898:CDS | 50.0% | |
| TGTGGCATAGCAGGGTTCTT+TGG | + | chr8.2:31978921-31978940 | MS.gene067898:CDS | 50.0% | |
| TTGGCCTCTTGTGAGGTGAA+TGG | + | chr8.2:31978822-31978841 | MS.gene067898:CDS | 50.0% | |
| ! | CTTTTACTTGGAGGGGTTGG+TGG | + | chr8.2:31978861-31978880 | MS.gene067898:CDS | 50.0% |
| ! | TTATAGCCGTGGTGGCTTTG+TGG | + | chr8.2:31979063-31979082 | MS.gene067898:CDS | 50.0% |
| !! | GTGTTGACTGTGGCATAGCA+GGG | + | chr8.2:31978913-31978932 | MS.gene067898:CDS | 50.0% |
| ! | GACTAGCCACAAAGCCACCA+CGG | - | chr8.2:31979072-31979091 | None:intergenic | 55.0% |
| !! | GGTGTTGACTGTGGCATAGC+AGG | + | chr8.2:31978912-31978931 | MS.gene067898:CDS | 55.0% |
| !!! | CTGGTTTTGTTGCTGGGAGC+TGG | + | chr8.2:31979030-31979049 | MS.gene067898:CDS | 55.0% |
| TTGCTGGGAGCTGGCTGCAG+CGG | + | chr8.2:31979039-31979058 | MS.gene067898:CDS | 65.0% | |
| !! | GCAGCGGGTTATAGCCGTGG+TGG | + | chr8.2:31979055-31979074 | MS.gene067898:CDS | 65.0% |
| !! | GCTGCAGCGGGTTATAGCCG+TGG | + | chr8.2:31979052-31979071 | MS.gene067898:CDS | 65.0% |
| TGCTGGGAGCTGGCTGCAGC+GGG | + | chr8.2:31979040-31979059 | MS.gene067898:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 31977504 | 31979105 | 31977504 | ID=MS.gene067898 |
| chr8.2 | mRNA | 31977504 | 31979105 | 31977504 | ID=MS.gene067898.t1;Parent=MS.gene067898 |
| chr8.2 | exon | 31977504 | 31977519 | 31977504 | ID=MS.gene067898.t1.exon1;Parent=MS.gene067898.t1 |
| chr8.2 | CDS | 31977504 | 31977519 | 31977504 | ID=cds.MS.gene067898.t1;Parent=MS.gene067898.t1 |
| chr8.2 | exon | 31977887 | 31977945 | 31977887 | ID=MS.gene067898.t1.exon2;Parent=MS.gene067898.t1 |
| chr8.2 | CDS | 31977887 | 31977945 | 31977887 | ID=cds.MS.gene067898.t1;Parent=MS.gene067898.t1 |
| chr8.2 | exon | 31978804 | 31978947 | 31978804 | ID=MS.gene067898.t1.exon3;Parent=MS.gene067898.t1 |
| chr8.2 | CDS | 31978804 | 31978947 | 31978804 | ID=cds.MS.gene067898.t1;Parent=MS.gene067898.t1 |
| chr8.2 | exon | 31978983 | 31979105 | 31978983 | ID=MS.gene067898.t1.exon4;Parent=MS.gene067898.t1 |
| chr8.2 | CDS | 31978983 | 31979105 | 31978983 | ID=cds.MS.gene067898.t1;Parent=MS.gene067898.t1 |
| Gene Sequence |
| Protein sequence |
