AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054582


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054582.t1 MTR_8g018100 70.000 130 18 2 101 227 87 198 3.09e-50 165
MS.gene054582.t1 MTR_8g018100 44.000 125 49 6 18 139 131 237 7.32e-13 66.6
MS.gene054582.t1 MTR_1g115220 80.882 68 10 1 157 221 21 88 1.04e-27 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGATGATTACCTTTGATTT+TGG 0.169382 4.1:+87209864 None:intergenic
AGATCTTCGCGAAGCTAAAT+TGG 0.247966 4.1:-87210767 MS.gene054582:CDS
CTAGTTGAATCAAAGCTTCT+TGG 0.282657 4.1:+87210249 None:intergenic
TCGCTTTCTAAATTTATATT+CGG 0.331894 4.1:-87210860 MS.gene054582:CDS
CCGAAGGATCATCCATTATA+CGG 0.375242 4.1:-87210886 MS.gene054582:CDS
TGCGTGTCAACGGGTGCAAC+AGG 0.392472 4.1:+87209904 None:intergenic
AGGAGAAGAACGAACCATTT+GGG 0.393055 4.1:+87210197 None:intergenic
AATCAAAGCTTCTTGGGTAA+TGG 0.393707 4.1:+87210256 None:intergenic
TCTTCCACCTCTCCTGGTGC+AGG 0.394878 4.1:+87209401 None:intergenic
AGAAAGCGACAACCGTATAA+TGG 0.406074 4.1:+87210874 None:intergenic
GGGATATGATAAGGAAGATA+AGG 0.437427 4.1:-87209450 MS.gene054582:CDS
CTACAATTGTGCGTGTCAAC+GGG 0.442078 4.1:+87209895 None:intergenic
ATGGCAGTGCTTTCCCCAAA+TGG 0.446026 4.1:-87210211 MS.gene054582:intron
CTTCCACCTCTCCTGGTGCA+GGG 0.450689 4.1:+87209402 None:intergenic
GTTAACTCTTCCACCTCTCC+TGG 0.451248 4.1:+87209395 None:intergenic
AGACTAAAATTTCACCTCTC+AGG 0.451846 4.1:+87210688 None:intergenic
CCTTGCCGCCACCATGGACT+TGG 0.453782 4.1:-87210914 None:intergenic
CCGTATAATGGATGATCCTT+CGG 0.457729 4.1:+87210886 None:intergenic
AAAAGAACCAACTGCGAAAA+GGG 0.458718 4.1:-87209588 MS.gene054582:CDS
TAGTTGAATCAAAGCTTCTT+GGG 0.458718 4.1:+87210250 None:intergenic
AAAAGAACCAACTGCGAAAA+GGG 0.458718 4.1:-87210579 MS.gene054582:CDS
ATATTCGGACGAGGCACTCT+TGG 0.459564 4.1:-87210845 MS.gene054582:CDS
TCACAAATCTTCTTTGCTGC+AGG 0.460278 4.1:+87210335 None:intergenic
GGATCAGGAGGGATATGATA+AGG 0.465606 4.1:-87209459 MS.gene054582:CDS
CAAAAGAACCAACTGCGAAA+AGG 0.465631 4.1:-87209589 MS.gene054582:CDS
CAAAAGAACCAACTGCGAAA+AGG 0.465631 4.1:-87210580 MS.gene054582:CDS
GGAAGGCCAATCTGATGATA+AGG 0.467371 4.1:-87209429 MS.gene054582:CDS
GCTACAATTGTGCGTGTCAA+CGG 0.476888 4.1:+87209894 None:intergenic
AGCCCCTGCACCAGGAGAGG+TGG 0.481468 4.1:-87209405 MS.gene054582:CDS
GGGCTTCCTTATCATCAGAT+TGG 0.481597 4.1:+87209423 None:intergenic
TTCCACCTCTCCTGGTGCAG+GGG 0.483421 4.1:+87209403 None:intergenic
GTTATATAAGTACGAAGATA+AGG 0.511157 4.1:-87210821 MS.gene054582:CDS
AGTTAACAGATGAGGAGTAG+TGG 0.515594 4.1:-87209379 None:intergenic
GATAAGGAAGCCCCTGCACC+AGG 0.518868 4.1:-87209413 MS.gene054582:CDS
CTGCGAAAAGGGCTAAAGTT+GGG 0.522171 4.1:-87210568 MS.gene054582:CDS
ACTGCGAAAAGGGCTAAAGT+TGG 0.541934 4.1:-87210569 MS.gene054582:CDS
ACTGCGAAAAGGGCTAAAGT+TGG 0.558971 4.1:-87209578 MS.gene054582:CDS
GGAAGCCCCTGCACCAGGAG+AGG 0.576902 4.1:-87209408 MS.gene054582:CDS
GGTGGAAGAGTTAACAGATG+AGG 0.581307 4.1:-87209387 MS.gene054582:CDS
AAAAGGGCTAAAGTTGGGAA+AGG 0.581404 4.1:-87210563 MS.gene054582:CDS
CTTCGGCAACTCCAAGTCCA+TGG 0.589099 4.1:+87210903 None:intergenic
TATTTATGTTTAGGATCAGG+AGG 0.589442 4.1:-87209471 MS.gene054582:intron
TCTAAATTTATATTCGGACG+AGG 0.591042 4.1:-87210854 MS.gene054582:CDS
AGCCTTGAAGCCAAAATCAA+AGG 0.601595 4.1:-87209874 MS.gene054582:intron
CATGGACTTGGAGTTGCCGA+AGG 0.601960 4.1:-87210902 MS.gene054582:CDS
CTAGAAATAAGTGACAAAGA+TGG 0.605930 4.1:-87210230 MS.gene054582:CDS
ATTTATGTTTAGGATCAGGA+GGG 0.610871 4.1:-87209470 MS.gene054582:intron
AAAGGGCTAAAGTTGGGAAA+GGG 0.618574 4.1:-87210562 MS.gene054582:CDS
TATGATAAGGAAGATAAGGA+AGG 0.621270 4.1:-87209446 MS.gene054582:CDS
GGAGAAGAACGAACCATTTG+GGG 0.661729 4.1:+87210198 None:intergenic
CTGAAGTGAAGAAACCTGAG+AGG 0.670696 4.1:-87210702 MS.gene054582:intron
ATATAAGTACGAAGATAAGG+TGG 0.691646 4.1:-87210818 MS.gene054582:CDS
CTGAAGTGAAGAAACCCGAG+AGG 0.719762 4.1:-87209721 MS.gene054582:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTTGATTTTATTTAATT+TGG - chr4.1:87210507-87210526 MS.gene054582:intron 10.0%
!!! TTTAGGTTTATTTTTTTTTT+TGG - chr4.1:87209656-87209675 MS.gene054582:intron 10.0%
!!! TTTTTTTTTTGTTAGTATTT+AGG - chr4.1:87209639-87209658 MS.gene054582:intron 10.0%
!! AATATTCACAAACTAAAATT+GGG + chr4.1:87210600-87210619 None:intergenic 15.0%
!!! AAGTATGTTTTCTTTTAATT+TGG - chr4.1:87209775-87209794 MS.gene054582:intron 15.0%
!!! AGTATGTTTTCTTTTAATTT+GGG - chr4.1:87209776-87209795 MS.gene054582:intron 15.0%
!!! TTGATAATAATTCCTAATTA+GGG + chr4.1:87210458-87210477 None:intergenic 15.0%
!!! TTTAGGTTTATCTATTTTTT+TGG - chr4.1:87210647-87210666 MS.gene054582:intron 15.0%
!!! TTTGATAATAATTCCTAATT+AGG + chr4.1:87210459-87210478 None:intergenic 15.0%
!!! TTTTTTTTTTGGAATTTTGT+AGG - chr4.1:87209667-87209686 MS.gene054582:intron 15.0%
!! ATATTCACAAACTAAAATTG+GGG + chr4.1:87210599-87210618 None:intergenic 20.0%
!! CAATATTCACAAACTAAAAT+TGG + chr4.1:87210601-87210620 None:intergenic 20.0%
!! TCGCTTTCTAAATTTATATT+CGG - chr4.1:87209423-87209442 MS.gene054582:CDS 20.0%
!!! CTATTTTTTTGGAATTTTGT+AGG - chr4.1:87210658-87210677 MS.gene054582:intron 20.0%
! AAAAATTCTCACAAAAGTCT+TGG - chr4.1:87209989-87210008 MS.gene054582:intron 25.0%
! ATTACTCATAAACCCTAATT+AGG - chr4.1:87210443-87210462 MS.gene054582:intron 25.0%
! GAATCATTTCTTCAAAAACA+TGG + chr4.1:87209923-87209942 None:intergenic 25.0%
! GTTATATAAGTACGAAGATA+AGG - chr4.1:87209462-87209481 MS.gene054582:intron 25.0%
! TTGAGTGTAATTATAAGTGT+CGG - chr4.1:87210185-87210204 MS.gene054582:intron 25.0%
! TTTCAAATTATTAGCTGTGT+CGG + chr4.1:87210153-87210172 None:intergenic 25.0%
!! CTATATTTATGTTTAGGATC+AGG - chr4.1:87210809-87210828 MS.gene054582:CDS 25.0%
!! TCTTGTTTGTGTTAGTATTT+AGG - chr4.1:87210624-87210643 MS.gene054582:intron 25.0%
!! TTGTGTTAGTATTTAGGTTT+AGG - chr4.1:87210630-87210649 MS.gene054582:intron 25.0%
!! TTTTAGTCTGTGAATATTGT+TGG - chr4.1:87209609-87209628 MS.gene054582:intron 25.0%
ATATAAGTACGAAGATAAGG+TGG - chr4.1:87209465-87209484 MS.gene054582:intron 30.0%
CTAGAAATAAGTGACAAAGA+TGG - chr4.1:87210053-87210072 MS.gene054582:intron 30.0%
TAGTTGAATCAAAGCTTCTT+GGG + chr4.1:87210036-87210055 None:intergenic 30.0%
TATGATAAGGAAGATAAGGA+AGG - chr4.1:87210837-87210856 MS.gene054582:CDS 30.0%
TCTAAATTTATATTCGGACG+AGG - chr4.1:87209429-87209448 MS.gene054582:CDS 30.0%
TGAGTGTAATTATAAGTGTC+GGG - chr4.1:87210186-87210205 MS.gene054582:intron 30.0%
! AGTTGGAGTATGTTTTCTTT+TGG - chr4.1:87210722-87210741 MS.gene054582:CDS 30.0%
! ATTTATGTTTAGGATCAGGA+GGG - chr4.1:87210813-87210832 MS.gene054582:CDS 30.0%
! GTTGGAGTATGTTTTCTTTT+GGG - chr4.1:87210723-87210742 MS.gene054582:CDS 30.0%
! TATTTATGTTTAGGATCAGG+AGG - chr4.1:87210812-87210831 MS.gene054582:CDS 30.0%
!! ACGATGATTACCTTTGATTT+TGG + chr4.1:87210422-87210441 None:intergenic 30.0%
!! CTGGTGCTATATTTATGTTT+AGG - chr4.1:87210803-87210822 MS.gene054582:CDS 30.0%
AAAAGAACCAACTGCGAAAA+GGG - chr4.1:87209704-87209723 MS.gene054582:intron 35.0%
AATCAAAGCTTCTTGGGTAA+TGG + chr4.1:87210030-87210049 None:intergenic 35.0%
AGACTAAAATTTCACCTCTC+AGG + chr4.1:87209598-87209617 None:intergenic 35.0%
CTAGTTGAATCAAAGCTTCT+TGG + chr4.1:87210037-87210056 None:intergenic 35.0%
GGGATATGATAAGGAAGATA+AGG - chr4.1:87210833-87210852 MS.gene054582:CDS 35.0%
! GAGTGTAATTATAAGTGTCG+GGG - chr4.1:87210187-87210206 MS.gene054582:intron 35.0%
! TTGTTTAAAATCGAAGCAGC+AGG - chr4.1:87209881-87209900 MS.gene054582:CDS 35.0%
!! GCAGTTGGTTCTTTTGAATT+TGG + chr4.1:87209699-87209718 None:intergenic 35.0%
!!! TACCTTTGATTTTGGCTTCA+AGG + chr4.1:87210414-87210433 None:intergenic 35.0%
AAAAGGGCTAAAGTTGGGAA+AGG - chr4.1:87209720-87209739 MS.gene054582:intron 40.0%
AAAGGGCTAAAGTTGGGAAA+GGG - chr4.1:87209721-87209740 MS.gene054582:intron 40.0%
AAGGAGAAGAACGAACCATT+TGG + chr4.1:87210090-87210109 None:intergenic 40.0%
AGAAAGCGACAACCGTATAA+TGG + chr4.1:87209412-87209431 None:intergenic 40.0%
AGATCTTCGCGAAGCTAAAT+TGG - chr4.1:87209516-87209535 MS.gene054582:intron 40.0%
AGCCTTGAAGCCAAAATCAA+AGG - chr4.1:87210409-87210428 MS.gene054582:intron 40.0%
AGGAGAAGAACGAACCATTT+GGG + chr4.1:87210089-87210108 None:intergenic 40.0%
CAAAAGAACCAACTGCGAAA+AGG - chr4.1:87209703-87209722 MS.gene054582:intron 40.0%
CCGAAGGATCATCCATTATA+CGG - chr4.1:87209397-87209416 MS.gene054582:CDS 40.0%
CTGTGAATATTGTTGGACTC+TGG - chr4.1:87209616-87209635 MS.gene054582:intron 40.0%
GGGAAAAAATTTCACCTCTC+GGG + chr4.1:87210579-87210598 None:intergenic 40.0%
GGGCTTCTATGAATTGTGTT+TGG - chr4.1:87209796-87209815 MS.gene054582:intron 40.0%
GGGGAAAAAATTTCACCTCT+CGG + chr4.1:87210580-87210599 None:intergenic 40.0%
TATGAAGCATGGACACTTCT+CGG + chr4.1:87210222-87210241 None:intergenic 40.0%
TCACAAATCTTCTTTGCTGC+AGG + chr4.1:87209951-87209970 None:intergenic 40.0%
! TTTAAAATCGAAGCAGCAGG+AGG - chr4.1:87209884-87209903 MS.gene054582:CDS 40.0%
!! CCGTATAATGGATGATCCTT+CGG + chr4.1:87209400-87209419 None:intergenic 40.0%
!! TGTAATTATAAGTGTCGGGG+CGG - chr4.1:87210190-87210209 MS.gene054582:intron 40.0%
!!! TTTGCTCTGTTTTCTGAAGC+TGG - chr4.1:87210784-87210803 MS.gene054582:CDS 40.0%
ACTGCGAAAAGGGCTAAAGT+TGG - chr4.1:87209714-87209733 MS.gene054582:intron 45.0%
CTACAATTGTGCGTGTCAAC+GGG + chr4.1:87210391-87210410 None:intergenic 45.0%
CTGAAGTGAAGAAACCTGAG+AGG - chr4.1:87209581-87209600 MS.gene054582:CDS 45.0%
CTGCGAAAAGGGCTAAAGTT+GGG - chr4.1:87209715-87209734 MS.gene054582:intron 45.0%
GATCAGAGTCCTATGAAGCA+TGG + chr4.1:87210233-87210252 None:intergenic 45.0%
GCTACAATTGTGCGTGTCAA+CGG + chr4.1:87210392-87210411 None:intergenic 45.0%
GGAAGGCCAATCTGATGATA+AGG - chr4.1:87210854-87210873 MS.gene054582:CDS 45.0%
GGAGAAGAACGAACCATTTG+GGG + chr4.1:87210088-87210107 None:intergenic 45.0%
GGATCAGGAGGGATATGATA+AGG - chr4.1:87210824-87210843 MS.gene054582:CDS 45.0%
GGGCTTCCTTATCATCAGAT+TGG + chr4.1:87210863-87210882 None:intergenic 45.0%
GGTGGAAGAGTTAACAGATG+AGG - chr4.1:87210896-87210915 MS.gene054582:CDS 45.0%
! ACTTTAGCCCTTTTCGCAGT+TGG + chr4.1:87209714-87209733 None:intergenic 45.0%
! TCTGGTGTCAGTAGAAGAGA+AGG + chr4.1:87210109-87210128 None:intergenic 45.0%
!! GAGAAGTGTCCATGCTTCAT+AGG - chr4.1:87210221-87210240 MS.gene054582:CDS 45.0%
ATGGCAGTGCTTTCCCCAAA+TGG - chr4.1:87210072-87210091 MS.gene054582:intron 50.0%
CTGAAGTGAAGAAACCCGAG+AGG - chr4.1:87210562-87210581 MS.gene054582:CDS 50.0%
GATTGTGTCCATGTCGTGTC+TGG + chr4.1:87210127-87210146 None:intergenic 50.0%
GCATGGACACTTCTCGGATT+AGG + chr4.1:87210216-87210235 None:intergenic 50.0%
GTTAACTCTTCCACCTCTCC+TGG + chr4.1:87210891-87210910 None:intergenic 50.0%
TACTGACACCAGACACGACA+TGG - chr4.1:87210116-87210135 MS.gene054582:intron 50.0%
! ATATTCGGACGAGGCACTCT+TGG - chr4.1:87209438-87209457 MS.gene054582:CDS 50.0%
CTTCCACCTCTCCTGGTGCA+GGG + chr4.1:87210884-87210903 None:intergenic 60.0%
GATAAGGAAGCCCCTGCACC+AGG - chr4.1:87210870-87210889 MS.gene054582:CDS 60.0%
TCTTCCACCTCTCCTGGTGC+AGG + chr4.1:87210885-87210904 None:intergenic 60.0%
TGCGTGTCAACGGGTGCAAC+AGG + chr4.1:87210382-87210401 None:intergenic 60.0%
TTCCACCTCTCCTGGTGCAG+GGG + chr4.1:87210883-87210902 None:intergenic 60.0%
AGCCCCTGCACCAGGAGAGG+TGG - chr4.1:87210878-87210897 MS.gene054582:CDS 70.0%
GGAAGCCCCTGCACCAGGAG+AGG - chr4.1:87210875-87210894 MS.gene054582:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 87209382 87210923 87209382 ID=MS.gene054582
chr4.1 mRNA 87209382 87210923 87209382 ID=MS.gene054582.t1;Parent=MS.gene054582
chr4.1 exon 87210703 87210923 87210703 ID=MS.gene054582.t1.exon1;Parent=MS.gene054582.t1
chr4.1 CDS 87210703 87210923 87210703 ID=cds.MS.gene054582.t1;Parent=MS.gene054582.t1
chr4.1 exon 87210529 87210616 87210529 ID=MS.gene054582.t1.exon2;Parent=MS.gene054582.t1
chr4.1 CDS 87210529 87210616 87210529 ID=cds.MS.gene054582.t1;Parent=MS.gene054582.t1
chr4.1 exon 87210212 87210405 87210212 ID=MS.gene054582.t1.exon3;Parent=MS.gene054582.t1
chr4.1 CDS 87210212 87210405 87210212 ID=cds.MS.gene054582.t1;Parent=MS.gene054582.t1
chr4.1 exon 87209875 87209948 87209875 ID=MS.gene054582.t1.exon4;Parent=MS.gene054582.t1
chr4.1 CDS 87209875 87209948 87209875 ID=cds.MS.gene054582.t1;Parent=MS.gene054582.t1
chr4.1 exon 87209722 87209767 87209722 ID=MS.gene054582.t1.exon5;Parent=MS.gene054582.t1
chr4.1 CDS 87209722 87209767 87209722 ID=cds.MS.gene054582.t1;Parent=MS.gene054582.t1
chr4.1 exon 87209577 87209625 87209577 ID=MS.gene054582.t1.exon6;Parent=MS.gene054582.t1
chr4.1 CDS 87209577 87209625 87209577 ID=cds.MS.gene054582.t1;Parent=MS.gene054582.t1
chr4.1 exon 87209382 87209480 87209382 ID=MS.gene054582.t1.exon7;Parent=MS.gene054582.t1
chr4.1 CDS 87209382 87209480 87209382 ID=cds.MS.gene054582.t1;Parent=MS.gene054582.t1
Gene Sequence

>MS.gene054582

ATGGACTTGGAGTTGCCGAAGGATCATCCATTATACGGTTGTCGCTTTCTAAATTTATATTCGGACGAGGCACTCTTGGAGTTATATAAGTACGAAGATAAGGTGGACGCTAAATTTGATCTGTGTCTTTTTGAAGATCTTCGCGAAGCTAAATTGGAAAAAGATGAACAAAATCTTTGTTTAGATTCTGAGCAAGCTTCTGAAGTGAAGAAACCTGAGAGGTGAAATTTTAGTCTGTGAATATTGTTGGACTCTGGTTTTTTTTTTGTTAGTATTTAGGTTTATTTTTTTTTTTGGAATTTTGTAGGAAGAAACCAAATTCAAAAGAACCAACTGCGAAAAGGGCTAAAGTTGGGAAAGGGAAAAATGTTAATGAATCTAATGCTGAAAATGAAGTATGTTTTCTTTTAATTTGGGCTTCTATGAATTGTGTTTGGTATTGTTAATTTTCAAAACAAAGCTAGTGTAGTGCTATTGAATTTGTTGTTAATTCGATTTGTTGTTTAAAATCGAAGCAGCAGGAGGCTTGTGCAGTATACCATGTTTTTGAAGAAATGATTCAGTTACCTGCAGCAAAGAAGATTTGTGATGAGCATAATGTTGATGAAAAAATTCTCACAAAAGTCTTGGAGAATTTGAAGAATTCCATTACCCAAGAAGCTTTGATTCAACTAGAAATAAGTGACAAAGATGGCAGTGCTTTCCCCAAATGGTTCGTTCTTCTCCTTCTCTTCTACTGACACCAGACACGACATGGACACAATCATGCCGACACAGCTAATAATTTGAAACAAATCATATTATTGAGTGTAATTATAAGTGTCGGGGCGGCCTAATCCGAGAAGTGTCCATGCTTCATAGGACTCTGATCTCTTTTTTTTTTTTTTTCTTTTCTCTCAGATTCTTCAATAGTTTTTTATTTTATTTACTAATTTTATGATTTTTTTTTATTTTTTTTTTTGTAAATTTTTTCAGTGATGAGAGTAAGAAAGATGTTCCTGTTGCACCCGTTGACACGCACAATTGTAGCCTTGAAGCCAAAATCAAAGGTAATCATCGTTATTACTCATAAACCCTAATTAGGAATTATTATCAAAATCTAAATAACTACGCTTGTTTATTTTCGTATTTGATTTTATTTAATTTGGTTGTTTAGATTCTCATGCTGTTGAGCAAGCTGCTGAAGTGAAGAAACCCGAGAGGTGAAATTTTTTCCCCAATTTTAGTTTGTGAATATTGAACTCTTGTTTGTGTTAGTATTTAGGTTTAGGTTTATCTATTTTTTTGGAATTTTGTAGGAAGAAACCAAATTCAAAAGAACCAACTGCGAAAAGGGCTAAAGTTGGAGTATGTTTTCTTTTGGGCTACTACTAAGTTTGTTTCATAATATGCTTTTGTTTATTTTGCTCTGTTTTCTGAAGCTGGTGCTATATTTATGTTTAGGATCAGGAGGGATATGATAAGGAAGATAAGGAAGGCCAATCTGATGATAAGGAAGCCCCTGCACCAGGAGAGGTGGAAGAGTTAACAGATGAGGAGTAG

Protein sequence

>MS.gene054582.t1

MDLELPKDHPLYGCRFLNLYSDEALLELYKYEDKVDAKFDLCLFEDLREAKLEKDEQNLCLDSEQASEVKKPERKKPNSKEPTAKRAKVGKGKNVNESNAENEQEACAVYHVFEEMIQLPAAKKICDEHNVDEKILTKVLENLKNSITQEALIQLEISDKDGSAFPKCDESKKDVPVAPVDTHNCSLEAKIKDSHAVEQAAEVKKPERKKPNSKEPTAKRAKVGDQEGYDKEDKEGQSDDKEAPAPGEVEELTDEE