AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053146


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053146.t1 MTR_8g018100 70.000 130 18 2 101 227 87 198 3.09e-50 165
MS.gene053146.t1 MTR_8g018100 44.000 125 49 6 18 139 131 237 7.32e-13 66.6
MS.gene053146.t1 MTR_1g115220 80.882 68 10 1 157 221 21 88 1.04e-27 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGATGATTACCTTTGATTT+TGG 0.169382 4.2:+91660604 None:intergenic
AGATCTTCGCGAAGCTAAAT+TGG 0.247966 4.2:-91661512 MS.gene053146:CDS
CTAGTTGAATCAAAGCTTCT+TGG 0.282657 4.2:+91660992 None:intergenic
TCGCTTTCTAAATTTATATT+CGG 0.331894 4.2:-91661605 MS.gene053146:CDS
CCGAAGGATCATCCATTATA+CGG 0.375242 4.2:-91661631 MS.gene053146:CDS
TGCGTGTCAACGGGTGCAAC+AGG 0.392472 4.2:+91660644 None:intergenic
AGGAGAAGAACGAACCATTT+GGG 0.393055 4.2:+91660940 None:intergenic
AATCAAAGCTTCTTGGGTAA+TGG 0.393707 4.2:+91660999 None:intergenic
TCTTCCACCTCTCCTGGTGC+AGG 0.394878 4.2:+91660141 None:intergenic
AGAAAGCGACAACCGTATAA+TGG 0.406074 4.2:+91661619 None:intergenic
GGGATATGATAAGGAAGATA+AGG 0.437427 4.2:-91660190 MS.gene053146:CDS
CTACAATTGTGCGTGTCAAC+GGG 0.442078 4.2:+91660635 None:intergenic
ATGGCAGTGCTTTCCCCAAA+TGG 0.446026 4.2:-91660954 MS.gene053146:intron
CTTCCACCTCTCCTGGTGCA+GGG 0.450689 4.2:+91660142 None:intergenic
GTTAACTCTTCCACCTCTCC+TGG 0.451248 4.2:+91660135 None:intergenic
AGACTAAAATTTCACCTCTC+AGG 0.451846 4.2:+91661433 None:intergenic
CCTTGCCGCCACCATGGACT+TGG 0.453782 4.2:-91661659 None:intergenic
CCGTATAATGGATGATCCTT+CGG 0.457729 4.2:+91661631 None:intergenic
AAAAGAACCAACTGCGAAAA+GGG 0.458718 4.2:-91660328 MS.gene053146:CDS
TAGTTGAATCAAAGCTTCTT+GGG 0.458718 4.2:+91660993 None:intergenic
AAAAGAACCAACTGCGAAAA+GGG 0.458718 4.2:-91661324 MS.gene053146:CDS
ATATTCGGACGAGGCACTCT+TGG 0.459564 4.2:-91661590 MS.gene053146:CDS
TCACAAATCTTCTTTGCTGC+AGG 0.460278 4.2:+91661078 None:intergenic
GGATCAGGAGGGATATGATA+AGG 0.465606 4.2:-91660199 MS.gene053146:CDS
CAAAAGAACCAACTGCGAAA+AGG 0.465631 4.2:-91660329 MS.gene053146:CDS
CAAAAGAACCAACTGCGAAA+AGG 0.465631 4.2:-91661325 MS.gene053146:CDS
GGAAGGCCAATCTGATGATA+AGG 0.467371 4.2:-91660169 MS.gene053146:CDS
GCTACAATTGTGCGTGTCAA+CGG 0.476888 4.2:+91660634 None:intergenic
AGCCCCTGCACCAGGAGAGG+TGG 0.481468 4.2:-91660145 MS.gene053146:CDS
GGGCTTCCTTATCATCAGAT+TGG 0.481597 4.2:+91660163 None:intergenic
TTCCACCTCTCCTGGTGCAG+GGG 0.483421 4.2:+91660143 None:intergenic
GTTATATAAGTACGAAGATA+AGG 0.511157 4.2:-91661566 MS.gene053146:CDS
AGTTAACAGATGAGGAGTAG+TGG 0.515594 4.2:-91660119 None:intergenic
GATAAGGAAGCCCCTGCACC+AGG 0.518868 4.2:-91660153 MS.gene053146:CDS
CTGCGAAAAGGGCTAAAGTT+GGG 0.522171 4.2:-91661313 MS.gene053146:CDS
ACTGCGAAAAGGGCTAAAGT+TGG 0.541934 4.2:-91661314 MS.gene053146:CDS
ACTGCGAAAAGGGCTAAAGT+TGG 0.558971 4.2:-91660318 MS.gene053146:CDS
GGAAGCCCCTGCACCAGGAG+AGG 0.576902 4.2:-91660148 MS.gene053146:CDS
GGTGGAAGAGTTAACAGATG+AGG 0.581307 4.2:-91660127 MS.gene053146:CDS
AAAAGGGCTAAAGTTGGGAA+AGG 0.581404 4.2:-91661308 MS.gene053146:CDS
CTTCGGCAACTCCAAGTCCA+TGG 0.589099 4.2:+91661648 None:intergenic
TATTTATGTTTAGGATCAGG+AGG 0.589442 4.2:-91660211 MS.gene053146:intron
TCTAAATTTATATTCGGACG+AGG 0.591042 4.2:-91661599 MS.gene053146:CDS
AGCCTTGAAGCCAAAATCAA+AGG 0.601595 4.2:-91660614 MS.gene053146:intron
CATGGACTTGGAGTTGCCGA+AGG 0.601960 4.2:-91661647 MS.gene053146:CDS
CTAGAAATAAGTGACAAAGA+TGG 0.605930 4.2:-91660973 MS.gene053146:CDS
ATTTATGTTTAGGATCAGGA+GGG 0.610871 4.2:-91660210 MS.gene053146:intron
AAAGGGCTAAAGTTGGGAAA+GGG 0.618574 4.2:-91661307 MS.gene053146:CDS
TATGATAAGGAAGATAAGGA+AGG 0.621270 4.2:-91660186 MS.gene053146:CDS
GGAGAAGAACGAACCATTTG+GGG 0.661729 4.2:+91660941 None:intergenic
CTGAAGTGAAGAAACCTGAG+AGG 0.670696 4.2:-91661447 MS.gene053146:intron
ATATAAGTACGAAGATAAGG+TGG 0.691646 4.2:-91661563 MS.gene053146:CDS
CTGAAGTGAAGAAACCCGAG+AGG 0.719762 4.2:-91660461 MS.gene053146:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTTGATTTTATTTAATT+TGG - chr4.2:91661252-91661271 MS.gene053146:intron 10.0%
!!! TTAGGTTTATTTATTTATTT+TGG - chr4.2:91660396-91660415 MS.gene053146:intron 10.0%
!! AATATTCACAAACTAAAATT+GGG + chr4.2:91661345-91661364 None:intergenic 15.0%
!!! AAGTATGTTTTCTTTTAATT+TGG - chr4.2:91660515-91660534 MS.gene053146:intron 15.0%
!!! GTTTTTTTTTGTTAGTATTT+AGG - chr4.2:91660378-91660397 MS.gene053146:intron 15.0%
!!! TATTTATTTTGGAATTTTGT+AGG - chr4.2:91660407-91660426 MS.gene053146:intron 15.0%
!!! TTGATAATAATTCCTAATTA+GGG + chr4.2:91661203-91661222 None:intergenic 15.0%
!!! TTTAGGTTTATCTATTTTTT+TGG - chr4.2:91661392-91661411 MS.gene053146:intron 15.0%
!!! TTTGATAATAATTCCTAATT+AGG + chr4.2:91661204-91661223 None:intergenic 15.0%
!! ATATTCACAAACTAAAATTG+GGG + chr4.2:91661344-91661363 None:intergenic 20.0%
!! CAATATTCACAAACTAAAAT+TGG + chr4.2:91661346-91661365 None:intergenic 20.0%
!! TCGCTTTCTAAATTTATATT+CGG - chr4.2:91660163-91660182 MS.gene053146:CDS 20.0%
!!! CAATTTTAGTTTGTGAATAT+TGG - chr4.2:91661344-91661363 MS.gene053146:intron 20.0%
!!! CTATTTTTTTGGAATTTTGT+AGG - chr4.2:91661403-91661422 MS.gene053146:intron 20.0%
! AAAAATTCTCACAAAAGTCT+TGG - chr4.2:91660731-91660750 MS.gene053146:intron 25.0%
! ATTACTCATAAACCCTAATT+AGG - chr4.2:91661188-91661207 MS.gene053146:intron 25.0%
! GAATCATTTCTTCAAAAACA+TGG + chr4.2:91660665-91660684 None:intergenic 25.0%
! GTTATATAAGTACGAAGATA+AGG - chr4.2:91660202-91660221 MS.gene053146:intron 25.0%
! TTTCAAATTATTAGCTGTGT+CGG + chr4.2:91660895-91660914 None:intergenic 25.0%
!! CTATATTTATGTTTAGGATC+AGG - chr4.2:91661554-91661573 MS.gene053146:CDS 25.0%
!! TCTTGTTTGTGTTAGTATTT+AGG - chr4.2:91661369-91661388 MS.gene053146:intron 25.0%
!! TTGTGTTAGTATTTAGGTTT+AGG - chr4.2:91661375-91661394 MS.gene053146:intron 25.0%
!! TTTTAGTCTGTGAATATTGT+TGG - chr4.2:91660349-91660368 MS.gene053146:intron 25.0%
ATATAAGTACGAAGATAAGG+TGG - chr4.2:91660205-91660224 MS.gene053146:intron 30.0%
CTAGAAATAAGTGACAAAGA+TGG - chr4.2:91660795-91660814 MS.gene053146:intron 30.0%
TACCAAACACAATTCATAGA+AGG + chr4.2:91660541-91660560 None:intergenic 30.0%
TAGTTGAATCAAAGCTTCTT+GGG + chr4.2:91660778-91660797 None:intergenic 30.0%
TATGATAAGGAAGATAAGGA+AGG - chr4.2:91661582-91661601 MS.gene053146:CDS 30.0%
TCTAAATTTATATTCGGACG+AGG - chr4.2:91660169-91660188 MS.gene053146:CDS 30.0%
TTGAGTGTAATTGTAAGTGT+CGG - chr4.2:91660927-91660946 MS.gene053146:intron 30.0%
! AGTTGGAGTATGTTTTCTTT+TGG - chr4.2:91661467-91661486 MS.gene053146:CDS 30.0%
! ATTTATGTTTAGGATCAGGA+GGG - chr4.2:91661558-91661577 MS.gene053146:CDS 30.0%
! GTTGGAGTATGTTTTCTTTT+GGG - chr4.2:91661468-91661487 MS.gene053146:CDS 30.0%
! TATTTATGTTTAGGATCAGG+AGG - chr4.2:91661557-91661576 MS.gene053146:CDS 30.0%
!! ACGATGATTACCTTTGATTT+TGG + chr4.2:91661167-91661186 None:intergenic 30.0%
!! CTGGTGCTATATTTATGTTT+AGG - chr4.2:91661548-91661567 MS.gene053146:CDS 30.0%
AAAAGAACCAACTGCGAAAA+GGG - chr4.2:91660444-91660463 MS.gene053146:intron 35.0%
AATCAAAGCTTCTTGGGTAA+TGG + chr4.2:91660772-91660791 None:intergenic 35.0%
AGACTAAAATTTCACCTCTC+AGG + chr4.2:91660338-91660357 None:intergenic 35.0%
CTAGTTGAATCAAAGCTTCT+TGG + chr4.2:91660779-91660798 None:intergenic 35.0%
GGGATATGATAAGGAAGATA+AGG - chr4.2:91661578-91661597 MS.gene053146:CDS 35.0%
TGAGTGTAATTGTAAGTGTC+GGG - chr4.2:91660928-91660947 MS.gene053146:intron 35.0%
TGTTTAAAAATCGAAGCAGC+AGG - chr4.2:91660623-91660642 MS.gene053146:CDS 35.0%
!! GCAGTTGGTTCTTTTGAATT+TGG + chr4.2:91660439-91660458 None:intergenic 35.0%
!!! TACCTTTGATTTTGGCTTCA+AGG + chr4.2:91661159-91661178 None:intergenic 35.0%
AAAAGGGCTAAAGTTGGGAA+AGG - chr4.2:91660460-91660479 MS.gene053146:intron 40.0%
AAAGGGCTAAAGTTGGGAAA+GGG - chr4.2:91660461-91660480 MS.gene053146:intron 40.0%
AAGGAGAAGAACGAACCATT+TGG + chr4.2:91660832-91660851 None:intergenic 40.0%
AGAAAGCGACAACCGTATAA+TGG + chr4.2:91660152-91660171 None:intergenic 40.0%
AGATCTTCGCGAAGCTAAAT+TGG - chr4.2:91660256-91660275 MS.gene053146:intron 40.0%
AGCCTTGAAGCCAAAATCAA+AGG - chr4.2:91661154-91661173 MS.gene053146:intron 40.0%
AGGAGAAGAACGAACCATTT+GGG + chr4.2:91660831-91660850 None:intergenic 40.0%
CAAAAGAACCAACTGCGAAA+AGG - chr4.2:91660443-91660462 MS.gene053146:intron 40.0%
CCGAAGGATCATCCATTATA+CGG - chr4.2:91660137-91660156 MS.gene053146:CDS 40.0%
CTGTGAATATTGTTGGACTC+TGG - chr4.2:91660356-91660375 MS.gene053146:intron 40.0%
GGCCTTCTATGAATTGTGTT+TGG - chr4.2:91660536-91660555 MS.gene053146:intron 40.0%
GGGAAAAAATTTCACCTCTC+GGG + chr4.2:91661324-91661343 None:intergenic 40.0%
GGGGAAAAAATTTCACCTCT+CGG + chr4.2:91661325-91661344 None:intergenic 40.0%
TATGAAGCATGGACACTTCT+CGG + chr4.2:91660964-91660983 None:intergenic 40.0%
TCACAAATCTTCTTTGCTGC+AGG + chr4.2:91660693-91660712 None:intergenic 40.0%
TTAAAAATCGAAGCAGCAGG+AGG - chr4.2:91660626-91660645 MS.gene053146:CDS 40.0%
! GAGTGTAATTGTAAGTGTCG+GGG - chr4.2:91660929-91660948 MS.gene053146:intron 40.0%
!! CCGTATAATGGATGATCCTT+CGG + chr4.2:91660140-91660159 None:intergenic 40.0%
!!! TTTGCTCTGTTTTCTGAAGC+TGG - chr4.2:91661529-91661548 MS.gene053146:CDS 40.0%
ACTGCGAAAAGGGCTAAAGT+TGG - chr4.2:91660454-91660473 MS.gene053146:intron 45.0%
CTACAATTGTGCGTGTCAAC+GGG + chr4.2:91661136-91661155 None:intergenic 45.0%
CTGAAGTGAAGAAACCTGAG+AGG - chr4.2:91660321-91660340 MS.gene053146:CDS 45.0%
CTGCGAAAAGGGCTAAAGTT+GGG - chr4.2:91660455-91660474 MS.gene053146:intron 45.0%
GATCAGAGTCCTATGAAGCA+TGG + chr4.2:91660975-91660994 None:intergenic 45.0%
GCTACAATTGTGCGTGTCAA+CGG + chr4.2:91661137-91661156 None:intergenic 45.0%
GGAAGGCCAATCTGATGATA+AGG - chr4.2:91661599-91661618 MS.gene053146:CDS 45.0%
GGAGAAGAACGAACCATTTG+GGG + chr4.2:91660830-91660849 None:intergenic 45.0%
GGATCAGGAGGGATATGATA+AGG - chr4.2:91661569-91661588 MS.gene053146:CDS 45.0%
GGGCTTCCTTATCATCAGAT+TGG + chr4.2:91661608-91661627 None:intergenic 45.0%
GGTGGAAGAGTTAACAGATG+AGG - chr4.2:91661641-91661660 MS.gene053146:CDS 45.0%
! ACTTTAGCCCTTTTCGCAGT+TGG + chr4.2:91660454-91660473 None:intergenic 45.0%
! TCTGGTGTCAGTAGAAGAGA+AGG + chr4.2:91660851-91660870 None:intergenic 45.0%
!! GAGAAGTGTCCATGCTTCAT+AGG - chr4.2:91660963-91660982 MS.gene053146:CDS 45.0%
!! TGTAATTGTAAGTGTCGGGG+CGG - chr4.2:91660932-91660951 MS.gene053146:intron 45.0%
ATGGCAGTGCTTTCCCCAAA+TGG - chr4.2:91660814-91660833 MS.gene053146:intron 50.0%
CTGAAGTGAAGAAACCCGAG+AGG - chr4.2:91661307-91661326 MS.gene053146:CDS 50.0%
GATTGTGTCCATGTCGTGTC+TGG + chr4.2:91660869-91660888 None:intergenic 50.0%
GCATGGACACTTCTCGGATT+AGG + chr4.2:91660958-91660977 None:intergenic 50.0%
GTTAACTCTTCCACCTCTCC+TGG + chr4.2:91661636-91661655 None:intergenic 50.0%
TACTGACACCAGACACGACA+TGG - chr4.2:91660858-91660877 MS.gene053146:intron 50.0%
! ATATTCGGACGAGGCACTCT+TGG - chr4.2:91660178-91660197 MS.gene053146:CDS 50.0%
CTTCCACCTCTCCTGGTGCA+GGG + chr4.2:91661629-91661648 None:intergenic 60.0%
GATAAGGAAGCCCCTGCACC+AGG - chr4.2:91661615-91661634 MS.gene053146:CDS 60.0%
TCTTCCACCTCTCCTGGTGC+AGG + chr4.2:91661630-91661649 None:intergenic 60.0%
TGCGTGTCAACGGGTGCAAC+AGG + chr4.2:91661127-91661146 None:intergenic 60.0%
TTCCACCTCTCCTGGTGCAG+GGG + chr4.2:91661628-91661647 None:intergenic 60.0%
AGCCCCTGCACCAGGAGAGG+TGG - chr4.2:91661623-91661642 MS.gene053146:CDS 70.0%
GGAAGCCCCTGCACCAGGAG+AGG - chr4.2:91661620-91661639 MS.gene053146:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 91660122 91661668 91660122 ID=MS.gene053146
chr4.2 mRNA 91660122 91661668 91660122 ID=MS.gene053146.t1;Parent=MS.gene053146
chr4.2 exon 91661448 91661668 91661448 ID=MS.gene053146.t1.exon1;Parent=MS.gene053146.t1
chr4.2 CDS 91661448 91661668 91661448 ID=cds.MS.gene053146.t1;Parent=MS.gene053146.t1
chr4.2 exon 91661274 91661361 91661274 ID=MS.gene053146.t1.exon2;Parent=MS.gene053146.t1
chr4.2 CDS 91661274 91661361 91661274 ID=cds.MS.gene053146.t1;Parent=MS.gene053146.t1
chr4.2 exon 91660955 91661148 91660955 ID=MS.gene053146.t1.exon3;Parent=MS.gene053146.t1
chr4.2 CDS 91660955 91661148 91660955 ID=cds.MS.gene053146.t1;Parent=MS.gene053146.t1
chr4.2 exon 91660615 91660688 91660615 ID=MS.gene053146.t1.exon4;Parent=MS.gene053146.t1
chr4.2 CDS 91660615 91660688 91660615 ID=cds.MS.gene053146.t1;Parent=MS.gene053146.t1
chr4.2 exon 91660462 91660507 91660462 ID=MS.gene053146.t1.exon5;Parent=MS.gene053146.t1
chr4.2 CDS 91660462 91660507 91660462 ID=cds.MS.gene053146.t1;Parent=MS.gene053146.t1
chr4.2 exon 91660317 91660365 91660317 ID=MS.gene053146.t1.exon6;Parent=MS.gene053146.t1
chr4.2 CDS 91660317 91660365 91660317 ID=cds.MS.gene053146.t1;Parent=MS.gene053146.t1
chr4.2 exon 91660122 91660220 91660122 ID=MS.gene053146.t1.exon7;Parent=MS.gene053146.t1
chr4.2 CDS 91660122 91660220 91660122 ID=cds.MS.gene053146.t1;Parent=MS.gene053146.t1
Gene Sequence

>MS.gene053146

ATGGACTTGGAGTTGCCGAAGGATCATCCATTATACGGTTGTCGCTTTCTAAATTTATATTCGGACGAGGCACTCTTGGAGTTATATAAGTACGAAGATAAGGTGGACGCTAAATTTGATCTGTGTCTTTTTGAAGATCTTCGCGAAGCTAAATTGGAAAAAGATGAACAAAATCTTTGTTTAGATTCTGAGCAAGCTTCTGAAGTGAAGAAACCTGAGAGGTGAAATTTTAGTCTGTGAATATTGTTGGACTCTGGTTTTTTTTTGTTAGTATTTAGGTTTATTTATTTATTTTGGAATTTTGTAGGAAGAAACCAAATTCAAAAGAACCAACTGCGAAAAGGGCTAAAGTTGGGAAAGGGAAAAATGTTAATGAATCTAATGCTGAAAATGAAGTATGTTTTCTTTTAATTTGGCCTTCTATGAATTGTGTTTGGTATTGTTAATTTTCAAAACAAAGCTAGTGTAGTGCTATTGAATTTGTTGTTAATTCGATTTTGTTGTTTAAAAATCGAAGCAGCAGGAGGCTTGTGCAGTATACCATGTTTTTGAAGAAATGATTCAGTTACCTGCAGCAAAGAAGATTTGTGATGAGCATAATGTTGATGAAAAAATTCTCACAAAAGTCTTGGAGAATTTGAAGAATTCCATTACCCAAGAAGCTTTGATTCAACTAGAAATAAGTGACAAAGATGGCAGTGCTTTCCCCAAATGGTTCGTTCTTCTCCTTCTCTTCTACTGACACCAGACACGACATGGACACAATCATGCCGACACAGCTAATAATTTGAAACAAATCATATTATTGAGTGTAATTGTAAGTGTCGGGGCGGCCTAATCCGAGAAGTGTCCATGCTTCATAGGACTCTGATCTCTCTTTTTTTTTTTTTTTCTTTTCTCTCAGATTCTTCAATAGTTTTTTATTTTATTTACTAATTTTATGATTTTTATTTTTTTTTTTTGTTTGTAAATTTTTTCAGTGATGAGAGTAAGAAAGATGTTCCTGTTGCACCCGTTGACACGCACAATTGTAGCCTTGAAGCCAAAATCAAAGGTAATCATCGTTATTACTCATAAACCCTAATTAGGAATTATTATCAAAATCTAAATAACTACGCTTGTTTATTTTCGTATTTGATTTTATTTAATTTGGTTGTTTAGATTCTCATGCTGTTGAGCAAGCTGCTGAAGTGAAGAAACCCGAGAGGTGAAATTTTTTCCCCAATTTTAGTTTGTGAATATTGGACTCTTGTTTGTGTTAGTATTTAGGTTTAGGTTTATCTATTTTTTTGGAATTTTGTAGGAAGAAACCAAATTCAAAAGAACCAACTGCGAAAAGGGCTAAAGTTGGAGTATGTTTTCTTTTGGGCTACTACTAAGTTTGTTTCATAATATGCTTTTGTTTATTTTGCTCTGTTTTCTGAAGCTGGTGCTATATTTATGTTTAGGATCAGGAGGGATATGATAAGGAAGATAAGGAAGGCCAATCTGATGATAAGGAAGCCCCTGCACCAGGAGAGGTGGAAGAGTTAACAGATGAGGAGTAG

Protein sequence

>MS.gene053146.t1

MDLELPKDHPLYGCRFLNLYSDEALLELYKYEDKVDAKFDLCLFEDLREAKLEKDEQNLCLDSEQASEVKKPERKKPNSKEPTAKRAKVGKGKNVNESNAENEQEACAVYHVFEEMIQLPAAKKICDEHNVDEKILTKVLENLKNSITQEALIQLEISDKDGSAFPKCDESKKDVPVAPVDTHNCSLEAKIKDSHAVEQAAEVKKPERKKPNSKEPTAKRAKVGDQEGYDKEDKEGQSDDKEAPAPGEVEELTDEE