Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068383.t1 | GAU47189.1 | 99.1 | 106 | 1 | 0 | 1 | 106 | 1 | 106 | 1.50E-56 | 228.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068383.t1 | Q9T096 | 66.7 | 102 | 34 | 0 | 1 | 102 | 1 | 102 | 1.7e-35 | 149.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068383.t1 | A0A2Z6PL80 | 99.1 | 106 | 1 | 0 | 1 | 106 | 1 | 106 | 1.1e-56 | 228.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene068383 | 0.808803 | 2.59E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene068383 | 0.829713 | 4.41E-55 | -1.69E-46 |
| MS.gene050559 | MS.gene068383 | 0.85848 | 8.00E-63 | -1.69E-46 |
| MS.gene050561 | MS.gene068383 | 0.841223 | 5.41E-58 | -1.69E-46 |
| MS.gene050562 | MS.gene068383 | 0.845403 | 4.15E-59 | -1.69E-46 |
| MS.gene051201 | MS.gene068383 | 0.814266 | 1.68E-51 | -1.69E-46 |
| MS.gene051279 | MS.gene068383 | 0.822948 | 1.80E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene068383 | 0.820488 | 6.68E-53 | -1.69E-46 |
| MS.gene052533 | MS.gene068383 | 0.835171 | 1.96E-56 | -1.69E-46 |
| MS.gene05686 | MS.gene068383 | 0.847723 | 9.66E-60 | -1.69E-46 |
| MS.gene057102 | MS.gene068383 | 0.819728 | 9.97E-53 | -1.69E-46 |
| MS.gene059634 | MS.gene068383 | 0.802609 | 5.17E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene068383 | -0.833732 | 4.50E-56 | -1.69E-46 |
| MS.gene060525 | MS.gene068383 | 0.880815 | 4.02E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068383.t1 | MTR_8g027255 | 99.057 | 106 | 1 | 0 | 1 | 106 | 1 | 106 | 9.92e-77 | 221 |
| MS.gene068383.t1 | MTR_3g091840 | 68.571 | 105 | 33 | 0 | 1 | 105 | 1 | 105 | 5.67e-51 | 156 |
| MS.gene068383.t1 | MTR_1g077750 | 64.835 | 91 | 32 | 0 | 1 | 91 | 1 | 91 | 2.82e-43 | 137 |
| MS.gene068383.t1 | MTR_5g082490 | 49.495 | 99 | 50 | 0 | 8 | 106 | 12 | 110 | 6.70e-33 | 111 |
| MS.gene068383.t1 | MTR_3g070330 | 51.064 | 94 | 46 | 0 | 8 | 101 | 35 | 128 | 1.16e-32 | 111 |
| MS.gene068383.t1 | MTR_3g070330 | 51.648 | 91 | 44 | 0 | 8 | 98 | 35 | 125 | 1.95e-31 | 107 |
| MS.gene068383.t1 | MTR_4g088505 | 49.000 | 100 | 50 | 1 | 3 | 102 | 8 | 106 | 3.30e-30 | 104 |
| MS.gene068383.t1 | MTR_7g068250 | 47.423 | 97 | 51 | 0 | 10 | 106 | 14 | 110 | 2.28e-29 | 102 |
| MS.gene068383.t1 | MTR_2g078670 | 47.368 | 95 | 50 | 0 | 8 | 102 | 12 | 106 | 3.34e-28 | 99.8 |
| MS.gene068383.t1 | MTR_8g058785 | 43.158 | 95 | 54 | 0 | 8 | 102 | 12 | 106 | 8.29e-26 | 93.6 |
| MS.gene068383.t1 | MTR_8g061270 | 43.158 | 95 | 54 | 0 | 8 | 102 | 12 | 106 | 8.29e-26 | 93.6 |
| MS.gene068383.t1 | MTR_1g077760 | 69.091 | 55 | 17 | 0 | 44 | 98 | 1 | 55 | 6.26e-21 | 81.3 |
| MS.gene068383.t1 | MTR_4g088505 | 46.835 | 79 | 41 | 1 | 3 | 81 | 8 | 85 | 1.13e-20 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068383.t1 | AT4G27745 | 66.667 | 102 | 34 | 0 | 1 | 102 | 1 | 102 | 1.53e-47 | 147 |
| MS.gene068383.t1 | AT4G27745 | 66.667 | 102 | 34 | 0 | 1 | 102 | 1 | 102 | 1.53e-47 | 147 |
| MS.gene068383.t1 | AT4G27745 | 66.667 | 102 | 34 | 0 | 1 | 102 | 1 | 102 | 1.53e-47 | 147 |
| MS.gene068383.t1 | AT4G27745 | 66.667 | 102 | 34 | 0 | 1 | 102 | 1 | 102 | 1.53e-47 | 147 |
| MS.gene068383.t1 | AT3G11230 | 51.515 | 99 | 48 | 0 | 8 | 106 | 12 | 110 | 4.92e-35 | 117 |
| MS.gene068383.t1 | AT3G11230 | 53.191 | 94 | 44 | 0 | 8 | 101 | 45 | 138 | 1.56e-34 | 116 |
| MS.gene068383.t1 | AT3G11230 | 53.261 | 92 | 43 | 0 | 8 | 99 | 12 | 103 | 6.83e-34 | 113 |
| MS.gene068383.t1 | AT3G11230 | 53.846 | 91 | 42 | 0 | 8 | 98 | 45 | 135 | 1.78e-33 | 113 |
| MS.gene068383.t1 | AT4G27740 | 51.064 | 94 | 46 | 0 | 9 | 102 | 9 | 102 | 3.39e-33 | 111 |
| MS.gene068383.t1 | AT3G55890 | 51.485 | 101 | 48 | 1 | 1 | 101 | 6 | 105 | 1.12e-32 | 110 |
| MS.gene068383.t1 | AT3G55890 | 51.485 | 101 | 48 | 1 | 1 | 101 | 6 | 105 | 1.12e-32 | 110 |
| MS.gene068383.t1 | AT5G53940 | 49.474 | 95 | 48 | 0 | 8 | 102 | 12 | 106 | 7.68e-32 | 108 |
| MS.gene068383.t1 | AT3G55890 | 51.546 | 97 | 46 | 1 | 1 | 97 | 6 | 101 | 1.49e-31 | 107 |
| MS.gene068383.t1 | AT3G55890 | 51.546 | 97 | 46 | 1 | 1 | 97 | 6 | 101 | 1.49e-31 | 107 |
| MS.gene068383.t1 | AT2G40110 | 48.485 | 99 | 51 | 0 | 8 | 106 | 12 | 110 | 1.70e-31 | 108 |
| MS.gene068383.t1 | AT3G08990 | 49.020 | 102 | 51 | 1 | 1 | 102 | 6 | 106 | 3.29e-31 | 107 |
| MS.gene068383.t1 | AT3G08990 | 49.020 | 102 | 51 | 1 | 1 | 102 | 6 | 106 | 3.29e-31 | 107 |
| MS.gene068383.t1 | AT3G08990 | 49.020 | 102 | 51 | 1 | 1 | 102 | 6 | 106 | 3.29e-31 | 107 |
| MS.gene068383.t1 | AT2G40110 | 48.913 | 92 | 47 | 0 | 8 | 99 | 12 | 103 | 3.56e-30 | 103 |
| MS.gene068383.t1 | AT2G40110 | 43.836 | 73 | 41 | 0 | 8 | 80 | 12 | 84 | 5.21e-21 | 80.5 |
| MS.gene068383.t1 | AT2G40110 | 43.836 | 73 | 41 | 0 | 8 | 80 | 12 | 84 | 5.21e-21 | 80.5 |
Find 20 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTTTCCTCATAAGCTTTA+AGG | 0.102549 | 8.3:-67128333 | None:intergenic |
| TGAGAAGTTTAAGATTGTTT+TGG | 0.258269 | 8.3:+67128384 | MS.gene068383:CDS |
| AAAGTTCACCTGAAAATCTT+TGG | 0.258418 | 8.3:-67127429 | None:intergenic |
| ATAAGCTGTCTATCTTCTTT+AGG | 0.260634 | 8.3:-67128244 | None:intergenic |
| ATCATTAATGGCCGATTTGA+TGG | 0.329137 | 8.3:+67127343 | None:intergenic |
| CATGGAAAGCAACAAGGTTC+TGG | 0.376925 | 8.3:-67127394 | None:intergenic |
| TGAGGAAACACAGAAGTATA+AGG | 0.390368 | 8.3:+67128345 | MS.gene068383:CDS |
| GAAATACCTTAAAGCTTATG+AGG | 0.433394 | 8.3:+67128327 | MS.gene068383:CDS |
| GGCCGATTTGATGGGATCAC+GGG | 0.488237 | 8.3:+67127352 | MS.gene068383:CDS |
| TGCCCGTGATCCCATCAAAT+CGG | 0.500160 | 8.3:-67127354 | None:intergenic |
| GATGTTTATTGTTCTGATTG+TGG | 0.528378 | 8.3:+67128289 | MS.gene068383:CDS |
| TCATTAATGGCCGATTTGAT+GGG | 0.534012 | 8.3:+67127344 | None:intergenic |
| TGGCCGATTTGATGGGATCA+CGG | 0.537450 | 8.3:+67127351 | MS.gene068383:CDS |
| TATGTTTAACAGGCAAGCAA+TGG | 0.540111 | 8.3:+67128178 | MS.gene068383:intron |
| CTTTGGAAACAATATCATCA+TGG | 0.545782 | 8.3:-67127412 | None:intergenic |
| TCTGATTGTGGAGAAGAACT+TGG | 0.556503 | 8.3:+67128301 | MS.gene068383:CDS |
| ATTGTGGAGAAGAACTTGGT+TGG | 0.558548 | 8.3:+67128305 | MS.gene068383:CDS |
| GAAGATAGACAGCTTATGAC+TGG | 0.563935 | 8.3:+67128250 | MS.gene068383:CDS |
| GAAACACAGAAGTATAAGGA+AGG | 0.612715 | 8.3:+67128349 | MS.gene068383:CDS |
| TATCATCATGGAAAGCAACA+AGG | 0.636612 | 8.3:-67127400 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAATTTGTTTAAATCAAA+TGG | + | chr8.3:67127584-67127603 | MS.gene068383:intron | 10.0% |
| !!! | AATTTACAATATGATTTTTT+TGG | - | chr8.3:67127995-67128014 | None:intergenic | 10.0% |
| !!! | TTAATTTGTTGAATATTAAT+TGG | + | chr8.3:67128092-67128111 | MS.gene068383:intron | 10.0% |
| !!! | AATTTTTTCTCTAAATCTAA+CGG | - | chr8.3:67127571-67127590 | None:intergenic | 15.0% |
| !!! | ACTTTTCTTTCTTTCAATTT+TGG | + | chr8.3:67127447-67127466 | MS.gene068383:intron | 20.0% |
| ! | AGAAGAAATGAAACAATCAT+TGG | + | chr8.3:67127894-67127913 | MS.gene068383:intron | 25.0% |
| ! | AGAGAAGTGAAATTAATTTC+AGG | - | chr8.3:67127805-67127824 | None:intergenic | 25.0% |
| ! | ATTAATTTCACTTCTCTCAA+TGG | + | chr8.3:67127808-67127827 | MS.gene068383:intron | 25.0% |
| ! | TGAATATTAATTGGACAAGA+GGG | + | chr8.3:67128101-67128120 | MS.gene068383:intron | 25.0% |
| ! | TTCATTTCTTCTGATCAATT+CGG | - | chr8.3:67127886-67127905 | None:intergenic | 25.0% |
| ! | TTGAATATTAATTGGACAAG+AGG | + | chr8.3:67128100-67128119 | MS.gene068383:intron | 25.0% |
| ! | TTTCAAAGGTTATGTTTAAC+AGG | + | chr8.3:67128168-67128187 | MS.gene068383:intron | 25.0% |
| !! | TAACCTCAAAAGATTTTCAA+AGG | + | chr8.3:67128154-67128173 | MS.gene068383:intron | 25.0% |
| !! | TATTAGATGATCCATTTTCA+TGG | - | chr8.3:67127520-67127539 | None:intergenic | 25.0% |
| !! | TATTGTTTCCAAAGATTTTC+AGG | + | chr8.3:67127421-67127440 | MS.gene068383:CDS | 25.0% |
| !!! | GAGAAGTTTAAGATTGTTTT+GGG | + | chr8.3:67128385-67128404 | MS.gene068383:CDS | 25.0% |
| !!! | TAACCTTTGAAAATCTTTTG+AGG | - | chr8.3:67128160-67128179 | None:intergenic | 25.0% |
| !!! | TGAGAAGTTTAAGATTGTTT+TGG | + | chr8.3:67128384-67128403 | MS.gene068383:CDS | 25.0% |
| !!! | TTAAGATTGTTTTGGGAAAT+GGG | + | chr8.3:67128392-67128411 | MS.gene068383:CDS | 25.0% |
| !!! | TTTAAGATTGTTTTGGGAAA+TGG | + | chr8.3:67128391-67128410 | MS.gene068383:CDS | 25.0% |
| AAACGATTCATCCATGAAAA+TGG | + | chr8.3:67127506-67127525 | MS.gene068383:intron | 30.0% | |
| AAAGTTCACCTGAAAATCTT+TGG | - | chr8.3:67127432-67127451 | None:intergenic | 30.0% | |
| AAATGGATCATCTAATACTG+TGG | + | chr8.3:67127523-67127542 | MS.gene068383:intron | 30.0% | |
| AGCTTTCATCGTGATTATAT+TGG | - | chr8.3:67127769-67127788 | None:intergenic | 30.0% | |
| ATAAGCTGTCTATCTTCTTT+AGG | - | chr8.3:67128247-67128266 | None:intergenic | 30.0% | |
| GAAATACCTTAAAGCTTATG+AGG | + | chr8.3:67128327-67128346 | MS.gene068383:CDS | 30.0% | |
| GATGTTTATTGTTCTGATTG+TGG | + | chr8.3:67128289-67128308 | MS.gene068383:CDS | 30.0% | |
| GCTTTCATCGTGATTATATT+GGG | - | chr8.3:67127768-67127787 | None:intergenic | 30.0% | |
| TAAAGGAAATGGACAAAATC+TGG | - | chr8.3:67127949-67127968 | None:intergenic | 30.0% | |
| ! | AGATTTTGTCCATTTCCTTT+AGG | + | chr8.3:67127948-67127967 | MS.gene068383:intron | 30.0% |
| ! | CATGCCATGAACATATTTTT+GGG | + | chr8.3:67128220-67128239 | MS.gene068383:CDS | 30.0% |
| ! | CTTTGGAAACAATATCATCA+TGG | - | chr8.3:67127415-67127434 | None:intergenic | 30.0% |
| ! | TCATGCCATGAACATATTTT+TGG | + | chr8.3:67128219-67128238 | MS.gene068383:CDS | 30.0% |
| !!! | TTGGTTTTAATTTAACCCTC+TGG | - | chr8.3:67127976-67127995 | None:intergenic | 30.0% |
| ACCCAAAAAATGTCTTCTAG+TGG | - | chr8.3:67127667-67127686 | None:intergenic | 35.0% | |
| AGGTCCCAAAAATATGTTCA+TGG | - | chr8.3:67128227-67128246 | None:intergenic | 35.0% | |
| ATATTAATTGGACAAGAGGG+TGG | + | chr8.3:67128104-67128123 | MS.gene068383:intron | 35.0% | |
| CCCAAAAAATGTCTTCTAGT+GGG | - | chr8.3:67127666-67127685 | None:intergenic | 35.0% | |
| GTGTTTCCTCATAAGCTTTA+AGG | - | chr8.3:67128336-67128355 | None:intergenic | 35.0% | |
| TATCATCATGGAAAGCAACA+AGG | - | chr8.3:67127403-67127422 | None:intergenic | 35.0% | |
| TATGTTTAACAGGCAAGCAA+TGG | + | chr8.3:67128178-67128197 | MS.gene068383:intron | 35.0% | |
| TGCTTAACGAGCTTCAATTA+AGG | + | chr8.3:67127860-67127879 | MS.gene068383:intron | 35.0% | |
| ! | AAAATGTCTTCTAGTGGGTA+TGG | - | chr8.3:67127661-67127680 | None:intergenic | 35.0% |
| ! | GAAACACAGAAGTATAAGGA+AGG | + | chr8.3:67128349-67128368 | MS.gene068383:CDS | 35.0% |
| ! | TGAGGAAACACAGAAGTATA+AGG | + | chr8.3:67128345-67128364 | MS.gene068383:CDS | 35.0% |
| !!! | ACCCACTAGAAGACATTTTT+TGG | + | chr8.3:67127662-67127681 | MS.gene068383:intron | 35.0% |
| !!! | CCCACTAGAAGACATTTTTT+GGG | + | chr8.3:67127663-67127682 | MS.gene068383:intron | 35.0% |
| AATCAAATGGTTCCTACCAC+AGG | + | chr8.3:67127597-67127616 | MS.gene068383:intron | 40.0% | |
| ATCAAATGGTTCCTACCACA+GGG | + | chr8.3:67127598-67127617 | MS.gene068383:intron | 40.0% | |
| ATTGTGGAGAAGAACTTGGT+TGG | + | chr8.3:67128305-67128324 | MS.gene068383:CDS | 40.0% | |
| CAGGGATGTTCTTCTACTTA+TGG | + | chr8.3:67127616-67127635 | MS.gene068383:intron | 40.0% | |
| GAAGATAGACAGCTTATGAC+TGG | + | chr8.3:67128250-67128269 | MS.gene068383:CDS | 40.0% | |
| TAAGTAGAAGAACATCCCTG+TGG | - | chr8.3:67127616-67127635 | None:intergenic | 40.0% | |
| TCTGATTGTGGAGAAGAACT+TGG | + | chr8.3:67128301-67128320 | MS.gene068383:CDS | 40.0% | |
| TGGGTATGGATGAACGAAAT+TGG | - | chr8.3:67127647-67127666 | None:intergenic | 40.0% | |
| ! | CATTTCCTTTAGGAACCAGA+GGG | + | chr8.3:67127958-67127977 | MS.gene068383:intron | 40.0% |
| ! | TTTAACCCTCTGGTTCCTAA+AGG | - | chr8.3:67127966-67127985 | None:intergenic | 40.0% |
| CATGGAAAGCAACAAGGTTC+TGG | - | chr8.3:67127397-67127416 | None:intergenic | 45.0% | |
| TAATTGGACAAGAGGGTGGT+TGG | + | chr8.3:67128108-67128127 | MS.gene068383:intron | 45.0% | |
| TAGAAGAACATCCCTGTGGT+AGG | - | chr8.3:67127612-67127631 | None:intergenic | 45.0% | |
| ! | ACTGTGGAATAGTGTTGCTG+CGG | + | chr8.3:67127539-67127558 | MS.gene068383:intron | 45.0% |
| ! | CCATTTCCTTTAGGAACCAG+AGG | + | chr8.3:67127957-67127976 | MS.gene068383:intron | 45.0% |
| ! | CCTCTGGTTCCTAAAGGAAA+TGG | - | chr8.3:67127960-67127979 | None:intergenic | 45.0% |
| TGCCCGTGATCCCATCAAAT+CGG | - | chr8.3:67127357-67127376 | None:intergenic | 50.0% | |
| !! | TGGCCGATTTGATGGGATCA+CGG | + | chr8.3:67127351-67127370 | MS.gene068383:CDS | 50.0% |
| !! | GGCCGATTTGATGGGATCAC+GGG | + | chr8.3:67127352-67127371 | MS.gene068383:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 67127350 | 67128417 | 67127350 | ID=MS.gene068383 |
| chr8.3 | mRNA | 67127350 | 67128417 | 67127350 | ID=MS.gene068383.t1;Parent=MS.gene068383 |
| chr8.3 | exon | 67127350 | 67127442 | 67127350 | ID=MS.gene068383.t1.exon1;Parent=MS.gene068383.t1 |
| chr8.3 | CDS | 67127350 | 67127442 | 67127350 | ID=cds.MS.gene068383.t1;Parent=MS.gene068383.t1 |
| chr8.3 | exon | 67128190 | 67128417 | 67128190 | ID=MS.gene068383.t1.exon2;Parent=MS.gene068383.t1 |
| chr8.3 | CDS | 67128190 | 67128417 | 67128190 | ID=cds.MS.gene068383.t1;Parent=MS.gene068383.t1 |
| Gene Sequence |
| Protein sequence |
