AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060525


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060525.t1 MTR_8g027255 100.000 106 0 0 1 106 1 106 4.12e-77 222
MS.gene060525.t1 MTR_3g091840 67.619 105 34 0 1 105 1 105 1.81e-50 155
MS.gene060525.t1 MTR_1g077750 63.736 91 33 0 1 91 1 91 1.24e-42 135
MS.gene060525.t1 MTR_5g082490 50.505 99 49 0 8 106 12 110 1.75e-33 113
MS.gene060525.t1 MTR_3g070330 52.128 94 45 0 8 101 35 128 2.35e-33 113
MS.gene060525.t1 MTR_3g070330 52.747 91 43 0 8 98 35 125 4.37e-32 109
MS.gene060525.t1 MTR_4g088505 50.000 100 49 1 3 102 8 106 1.05e-30 105
MS.gene060525.t1 MTR_7g068250 48.454 97 50 0 10 106 14 110 5.58e-30 103
MS.gene060525.t1 MTR_2g078670 48.421 95 49 0 8 102 12 106 9.53e-29 100
MS.gene060525.t1 MTR_8g058785 44.211 95 53 0 8 102 12 106 2.42e-26 95.1
MS.gene060525.t1 MTR_8g061270 44.211 95 53 0 8 102 12 106 2.42e-26 95.1
MS.gene060525.t1 MTR_4g088505 48.101 79 40 1 3 81 8 85 3.41e-21 81.6
MS.gene060525.t1 MTR_1g077760 67.273 55 18 0 44 98 1 55 1.47e-20 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060525.t1 AT4G27745 66.667 102 34 0 1 102 1 102 1.56e-47 147
MS.gene060525.t1 AT4G27745 66.667 102 34 0 1 102 1 102 1.56e-47 147
MS.gene060525.t1 AT4G27745 66.667 102 34 0 1 102 1 102 1.56e-47 147
MS.gene060525.t1 AT4G27745 66.667 102 34 0 1 102 1 102 1.56e-47 147
MS.gene060525.t1 AT3G11230 50.505 99 49 0 8 106 12 110 1.18e-34 115
MS.gene060525.t1 AT3G11230 50.505 99 49 0 8 106 45 143 4.30e-34 115
MS.gene060525.t1 AT3G11230 52.174 92 44 0 8 99 12 103 1.69e-33 112
MS.gene060525.t1 AT4G27740 51.064 94 46 0 9 102 9 102 2.61e-33 112
MS.gene060525.t1 AT3G55890 52.475 101 47 1 1 101 6 105 3.09e-33 112
MS.gene060525.t1 AT3G55890 52.475 101 47 1 1 101 6 105 3.09e-33 112
MS.gene060525.t1 AT3G11230 52.747 91 43 0 8 98 45 135 5.78e-33 112
MS.gene060525.t1 AT5G53940 50.526 95 47 0 8 102 12 106 2.16e-32 110
MS.gene060525.t1 AT2G40110 49.495 99 50 0 8 106 12 110 3.76e-32 109
MS.gene060525.t1 AT3G55890 52.577 97 45 1 1 97 6 101 4.29e-32 108
MS.gene060525.t1 AT3G55890 52.577 97 45 1 1 97 6 101 4.29e-32 108
MS.gene060525.t1 AT3G08990 50.000 102 50 1 1 102 6 106 8.69e-32 108
MS.gene060525.t1 AT3G08990 50.000 102 50 1 1 102 6 106 8.69e-32 108
MS.gene060525.t1 AT3G08990 50.000 102 50 1 1 102 6 106 8.69e-32 108
MS.gene060525.t1 AT2G40110 50.000 92 46 0 8 99 12 103 8.60e-31 105
MS.gene060525.t1 AT2G40110 45.205 73 40 0 8 80 12 84 1.25e-21 82.0
MS.gene060525.t1 AT2G40110 45.205 73 40 0 8 80 12 84 1.25e-21 82.0

Find 19 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTTTCCTCATAAGCTTTA+AGG 0.102549 8.1:-75153013 None:intergenic
TGAGAAGTTTAAGATTGTTT+TGG 0.258269 8.1:+75153064 MS.gene060525:CDS
AAAGTTCACCTGAAAATCTT+TGG 0.258418 8.1:-75152112 None:intergenic
ATAAGCTGTCTATCTTCTTT+AGG 0.260634 8.1:-75152924 None:intergenic
ATCATTAATGGCCGATTTGA+TGG 0.329137 8.1:+75152026 None:intergenic
CATGGAAAGCAACAAGGTTC+TGG 0.376925 8.1:-75152077 None:intergenic
TGAGGAAACACAGAAGTATA+AGG 0.390368 8.1:+75153025 MS.gene060525:CDS
GAAATACCTTAAAGCTTATG+AGG 0.433394 8.1:+75153007 MS.gene060525:CDS
GGCCGATTTGATGGGATCAC+GGG 0.488237 8.1:+75152035 MS.gene060525:CDS
TGCCCGTGATCCCATCAAAT+CGG 0.500160 8.1:-75152037 None:intergenic
TCATTAATGGCCGATTTGAT+GGG 0.534012 8.1:+75152027 None:intergenic
TGGCCGATTTGATGGGATCA+CGG 0.537450 8.1:+75152034 MS.gene060525:CDS
TATGTTTAACAGGCAAGCAA+TGG 0.540111 8.1:+75152858 MS.gene060525:intron
CTTTGGAAACAATATCATCA+TGG 0.545782 8.1:-75152095 None:intergenic
TCTGATTGTGGAGAAGAACT+TGG 0.556503 8.1:+75152981 MS.gene060525:CDS
ATTGTGGAGAAGAACTTGGT+TGG 0.558548 8.1:+75152985 MS.gene060525:CDS
GAAGATAGACAGCTTATGAC+TGG 0.563935 8.1:+75152930 MS.gene060525:CDS
GAAACACAGAAGTATAAGGA+AGG 0.612715 8.1:+75153029 MS.gene060525:CDS
TATCATCATGGAAAGCAACA+AGG 0.636612 8.1:-75152083 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTTGTTTAAATCAAA+TGG + chr8.1:75152267-75152286 MS.gene060525:intron 10.0%
!!! AATTTACAATATGATTTTTT+TGG - chr8.1:75152675-75152694 None:intergenic 10.0%
!!! TTAATTTGTTGAATATTAAT+TGG + chr8.1:75152772-75152791 MS.gene060525:intron 10.0%
!!! AATTTTTTCTCTAAATCTAA+CGG - chr8.1:75152254-75152273 None:intergenic 15.0%
!!! ACTTTTCTTTCTTTCAATTT+TGG + chr8.1:75152130-75152149 MS.gene060525:intron 20.0%
! AGAAGAAATGAAACAATCAT+TGG + chr8.1:75152574-75152593 MS.gene060525:intron 25.0%
! AGAGAAGTGAAATTAATTTC+AGG - chr8.1:75152485-75152504 None:intergenic 25.0%
! ATTAATTTCACTTCTCTCAA+TGG + chr8.1:75152488-75152507 MS.gene060525:intron 25.0%
! TGAATATTAATTGGACAAGA+GGG + chr8.1:75152781-75152800 MS.gene060525:intron 25.0%
! TTCATTTCTTCTGATCAATT+CGG - chr8.1:75152566-75152585 None:intergenic 25.0%
! TTGAATATTAATTGGACAAG+AGG + chr8.1:75152780-75152799 MS.gene060525:intron 25.0%
! TTTCAAAGGTTATGTTTAAC+AGG + chr8.1:75152848-75152867 MS.gene060525:intron 25.0%
!! TAACCTCAAAAGATTTTCAA+AGG + chr8.1:75152834-75152853 MS.gene060525:intron 25.0%
!! TATTAGATGATCCATTTTCA+TGG - chr8.1:75152203-75152222 None:intergenic 25.0%
!! TATTGTTTCCAAAGATTTTC+AGG + chr8.1:75152104-75152123 MS.gene060525:CDS 25.0%
!!! GAGAAGTTTAAGATTGTTTT+GGG + chr8.1:75153065-75153084 MS.gene060525:CDS 25.0%
!!! TAACCTTTGAAAATCTTTTG+AGG - chr8.1:75152840-75152859 None:intergenic 25.0%
!!! TGAGAAGTTTAAGATTGTTT+TGG + chr8.1:75153064-75153083 MS.gene060525:CDS 25.0%
!!! TTAAGATTGTTTTGGGAAAT+GGG + chr8.1:75153072-75153091 MS.gene060525:CDS 25.0%
!!! TTTAAGATTGTTTTGGGAAA+TGG + chr8.1:75153071-75153090 MS.gene060525:CDS 25.0%
AAACGATTCATCCATGAAAA+TGG + chr8.1:75152189-75152208 MS.gene060525:intron 30.0%
AAAGTTCACCTGAAAATCTT+TGG - chr8.1:75152115-75152134 None:intergenic 30.0%
AAATGGATCATCTAATACTG+TGG + chr8.1:75152206-75152225 MS.gene060525:intron 30.0%
AGCTTTCATCGTGATTATAT+TGG - chr8.1:75152452-75152471 None:intergenic 30.0%
ATAAGCTGTCTATCTTCTTT+AGG - chr8.1:75152927-75152946 None:intergenic 30.0%
GAAATACCTTAAAGCTTATG+AGG + chr8.1:75153007-75153026 MS.gene060525:CDS 30.0%
GCTTTCATCGTGATTATATT+GGG - chr8.1:75152451-75152470 None:intergenic 30.0%
TAAAGGAAATGGACAAAATC+CGG - chr8.1:75152629-75152648 None:intergenic 30.0%
! CATGCCATGAACATATTTTT+GGG + chr8.1:75152900-75152919 MS.gene060525:CDS 30.0%
! CTTTGGAAACAATATCATCA+TGG - chr8.1:75152098-75152117 None:intergenic 30.0%
! TCATGCCATGAACATATTTT+TGG + chr8.1:75152899-75152918 MS.gene060525:CDS 30.0%
!!! GATGTTTTTTGTTCTGATTG+TGG + chr8.1:75152969-75152988 MS.gene060525:CDS 30.0%
!!! TTGGTTTTAATTTAACCCTC+TGG - chr8.1:75152656-75152675 None:intergenic 30.0%
ACCCAAAAAATGTCTTCTAG+TGG - chr8.1:75152350-75152369 None:intergenic 35.0%
AGGTCCCAAAAATATGTTCA+TGG - chr8.1:75152907-75152926 None:intergenic 35.0%
ATATTAATTGGACAAGAGGG+TGG + chr8.1:75152784-75152803 MS.gene060525:intron 35.0%
CCCAAAAAATGTCTTCTAGT+GGG - chr8.1:75152349-75152368 None:intergenic 35.0%
GTGTTTCCTCATAAGCTTTA+AGG - chr8.1:75153016-75153035 None:intergenic 35.0%
TATCATCATGGAAAGCAACA+AGG - chr8.1:75152086-75152105 None:intergenic 35.0%
TATGTTTAACAGGCAAGCAA+TGG + chr8.1:75152858-75152877 MS.gene060525:intron 35.0%
TGCTTAACGAGCTTCAATTA+AGG + chr8.1:75152540-75152559 MS.gene060525:intron 35.0%
! AAAATGTCTTCTAGTGGGTA+TGG - chr8.1:75152344-75152363 None:intergenic 35.0%
! GAAACACAGAAGTATAAGGA+AGG + chr8.1:75153029-75153048 MS.gene060525:CDS 35.0%
! GGATTTTGTCCATTTCCTTT+AGG + chr8.1:75152628-75152647 MS.gene060525:intron 35.0%
! TGAGGAAACACAGAAGTATA+AGG + chr8.1:75153025-75153044 MS.gene060525:CDS 35.0%
!!! ACCCACTAGAAGACATTTTT+TGG + chr8.1:75152345-75152364 MS.gene060525:intron 35.0%
!!! CCCACTAGAAGACATTTTTT+GGG + chr8.1:75152346-75152365 MS.gene060525:intron 35.0%
AATCAAATGGTTCCTACCAC+AGG + chr8.1:75152280-75152299 MS.gene060525:intron 40.0%
ATCAAATGGTTCCTACCACA+GGG + chr8.1:75152281-75152300 MS.gene060525:intron 40.0%
ATTGTGGAGAAGAACTTGGT+TGG + chr8.1:75152985-75153004 MS.gene060525:CDS 40.0%
CAGGGATGTTCTTCTACTTA+TGG + chr8.1:75152299-75152318 MS.gene060525:intron 40.0%
CTTACTTTGTGACTGAACTC+CGG + chr8.1:75152607-75152626 MS.gene060525:intron 40.0%
GAAGATAGACAGCTTATGAC+TGG + chr8.1:75152930-75152949 MS.gene060525:CDS 40.0%
TAAGTAGAAGAACATCCCTG+TGG - chr8.1:75152299-75152318 None:intergenic 40.0%
TCTGATTGTGGAGAAGAACT+TGG + chr8.1:75152981-75153000 MS.gene060525:CDS 40.0%
TGGGTATGGATGAACGAAAT+TGG - chr8.1:75152330-75152349 None:intergenic 40.0%
! CATTTCCTTTAGGAACCAGA+GGG + chr8.1:75152638-75152657 MS.gene060525:intron 40.0%
! TTTAACCCTCTGGTTCCTAA+AGG - chr8.1:75152646-75152665 None:intergenic 40.0%
CATGGAAAGCAACAAGGTTC+TGG - chr8.1:75152080-75152099 None:intergenic 45.0%
TAATTGGACAAGAGGGTGGT+TGG + chr8.1:75152788-75152807 MS.gene060525:intron 45.0%
TAGAAGAACATCCCTGTGGT+AGG - chr8.1:75152295-75152314 None:intergenic 45.0%
! ACTGTGGAATAGTGTTGCTG+CGG + chr8.1:75152222-75152241 MS.gene060525:intron 45.0%
! CCATTTCCTTTAGGAACCAG+AGG + chr8.1:75152637-75152656 MS.gene060525:intron 45.0%
! CCTCTGGTTCCTAAAGGAAA+TGG - chr8.1:75152640-75152659 None:intergenic 45.0%
TGCCCGTGATCCCATCAAAT+CGG - chr8.1:75152040-75152059 None:intergenic 50.0%
!! TGGCCGATTTGATGGGATCA+CGG + chr8.1:75152034-75152053 MS.gene060525:CDS 50.0%
!! GGCCGATTTGATGGGATCAC+GGG + chr8.1:75152035-75152054 MS.gene060525:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 75152033 75153097 75152033 ID=MS.gene060525
chr8.1 mRNA 75152033 75153097 75152033 ID=MS.gene060525.t1;Parent=MS.gene060525
chr8.1 exon 75152033 75152125 75152033 ID=MS.gene060525.t1.exon1;Parent=MS.gene060525.t1
chr8.1 CDS 75152033 75152125 75152033 ID=cds.MS.gene060525.t1;Parent=MS.gene060525.t1
chr8.1 exon 75152870 75153097 75152870 ID=MS.gene060525.t1.exon2;Parent=MS.gene060525.t1
chr8.1 CDS 75152870 75153097 75152870 ID=cds.MS.gene060525.t1;Parent=MS.gene060525.t1
Gene Sequence

>MS.gene060525

ATGGCCGATTTGATGGGATCACGGGCATACTGCTGCTTCAAGTGCCAGAACCTTGTTGCTTTCCATGATGATATTGTTTCCAAAGATTTTCAGGTGAACTTTTCTTTCTTTCAATTTTGGCTTTTTCTTCATCTTTATGCTAGATTTCTATATTGTAAACGATTCATCCATGAAAATGGATCATCTAATACTGTGGAATAGTGTTGCTGCGGTAGAAACCGTTAGATTTAGAGAAAAAATTTGTTTAAATCAAATGGTTCCTACCACAGGGATGTTCTTCTACTTATGGATGATCCAATTTCGTTCATCCATACCCACTAGAAGACATTTTTTGGGTTTAGAATAGAATCAGTAAAGAACAAGACTTTGAAGTTTACTTGCTGAGATGAAGATGAAGATTTATATAATAACTAAGCCCAATATAATCACGATGAAAGCTTTTCAATCATCCTGAAATTAATTTCACTTCTCTCAATGGAATCATTTCATTTCATATATTATAAATTATGCTTAACGAGCTTCAATTAAGGCCGAATTGATCAGAAGAAATGAAACAATCATTGGTCAGACTTTACTTACTTTGTGACTGAACTCCGGATTTTGTCCATTTCCTTTAGGAACCAGAGGGTTAAATTAAAACCAAAAAAATCATATTGTAAATTTTCATTCAAATTTGTTTGTAACATATGATAATGATACTATTGTTGATAGTTTTGTTGTTATCTTCTGCTGAAATGAATTAATTTGTTGAATATTAATTGGACAAGAGGGTGGTTGGTGAAGATATTTGAAGTTTCTTTTTAACCTCAAAAGATTTTCAAAGGTTATGTTTAACAGGCAAGCAATGGAAGAGCTTTTCTGTTTTCTCATGCCATGAACATATTTTTGGGACCTAAAGAAGATAGACAGCTTATGACTGGTCTTCACACAGTTGCTGATGTTTTTTGTTCTGATTGTGGAGAAGAACTTGGTTGGAAATACCTTAAAGCTTATGAGGAAACACAGAAGTATAAGGAAGGAAAATGTGTGCTTGAGAAGTTTAAGATTGTTTTGGGAAATGGGTAG

Protein sequence

>MS.gene060525.t1

MADLMGSRAYCCFKCQNLVAFHDDIVSKDFQASNGRAFLFSHAMNIFLGPKEDRQLMTGLHTVADVFCSDCGEELGWKYLKAYEETQKYKEGKCVLEKFKIVLGNG