Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069094.t1 | XP_003612898.1 | 90 | 120 | 12 | 0 | 1 | 120 | 1 | 120 | 8.20E-30 | 139.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069094.t1 | G7KF21 | 90.0 | 120 | 12 | 0 | 1 | 120 | 1 | 120 | 5.9e-30 | 139.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene069094 | 0.805209 | 1.49E-49 | -1.69E-46 |
| MS.gene049150 | MS.gene069094 | 0.812947 | 3.28E-51 | -1.69E-46 |
| MS.gene049153 | MS.gene069094 | 0.804903 | 1.73E-49 | -1.69E-46 |
| MS.gene05119 | MS.gene069094 | -0.808157 | 3.56E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene069094 | 0.802929 | 4.44E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene069094 | 0.806935 | 6.46E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene069094 | 0.807094 | 5.98E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene069094 | 0.81413 | 1.80E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene069094 | 0.810506 | 1.11E-50 | -1.69E-46 |
| MS.gene054542 | MS.gene069094 | 0.811885 | 5.60E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene069094 | 0.851879 | 6.66E-61 | -1.69E-46 |
| MS.gene056659 | MS.gene069094 | 0.800391 | 1.47E-48 | -1.69E-46 |
| MS.gene056723 | MS.gene069094 | 0.819359 | 1.21E-52 | -1.69E-46 |
| MS.gene058002 | MS.gene069094 | 0.802096 | 6.60E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene069094 | 0.804478 | 2.12E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene069094 | 0.810911 | 9.11E-51 | -1.69E-46 |
| MS.gene059285 | MS.gene069094 | 0.846764 | 1.77E-59 | -1.69E-46 |
| MS.gene059420 | MS.gene069094 | 0.803375 | 3.59E-49 | -1.69E-46 |
| MS.gene059899 | MS.gene069094 | 0.811508 | 6.76E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069094.t1 | MTR_5g430430 | 95.000 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 9.34e-80 | 230 |
| MS.gene069094.t1 | MTR_5g030290 | 95.000 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 9.34e-80 | 230 |
| MS.gene069094.t1 | MTR_5g030310 | 84.167 | 120 | 19 | 0 | 1 | 120 | 1 | 120 | 5.16e-69 | 203 |
| MS.gene069094.t1 | MTR_5g430440 | 83.333 | 120 | 20 | 0 | 1 | 120 | 1 | 120 | 5.77e-68 | 200 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCTCTTGAGTGCAGCTT+TGG | 0.250449 | 5.3:+19160011 | None:intergenic |
| TCACTGCTACTCTCAATTTC+AGG | 0.257468 | 5.3:+19159169 | None:intergenic |
| GAAGGCCCTGATGCAGATTC+AGG | 0.346257 | 5.3:-19159085 | MS.gene069094:CDS |
| TTGCTCTTGAGTGCAGCTTT+GGG | 0.361214 | 5.3:+19160012 | None:intergenic |
| CCCTTGCCTGAATCTGCATC+AGG | 0.393096 | 5.3:+19159079 | None:intergenic |
| GGCAATGTGTTGTGCAAGAC+TGG | 0.472681 | 5.3:-19159805 | MS.gene069094:CDS |
| TGTGTTATAACTAGGACTAC+TGG | 0.487878 | 5.3:-19159769 | MS.gene069094:CDS |
| TCTTGTGCATGCAGTGATGA+TGG | 0.527741 | 5.3:-19159202 | MS.gene069094:intron |
| AGGACTACTGGACCTGATGC+TGG | 0.536873 | 5.3:-19159757 | MS.gene069094:CDS |
| CAAAGCTGCACTCAAGAGCA+AGG | 0.561178 | 5.3:-19160010 | MS.gene069094:CDS |
| CCCTGATGCAGATTCAGGCA+AGG | 0.568742 | 5.3:-19159080 | MS.gene069094:CDS |
| GTTGGACATGTGTTATAACT+AGG | 0.581816 | 5.3:-19159777 | MS.gene069094:CDS |
| TCTGCAAGTGTGGTGAAGGA+TGG | 0.591787 | 5.3:-19159129 | MS.gene069094:CDS |
| CCTTGCCTGAATCTGCATCA+GGG | 0.592747 | 5.3:+19159080 | None:intergenic |
| ACATTCTGCAAGTGTGGTGA+AGG | 0.593456 | 5.3:-19159133 | MS.gene069094:CDS |
| CCTGATGCAGATTCAGGCAA+GGG | 0.596736 | 5.3:-19159079 | MS.gene069094:CDS |
| GTGTGTGACATACAGTGTGC+AGG | 0.603372 | 5.3:+19159702 | None:intergenic |
| ATTGAGAGTAGCAGTGACAA+TGG | 0.620823 | 5.3:-19159163 | MS.gene069094:CDS |
| GTGTTGTGCAAGACTGGTAA+AGG | 0.626364 | 5.3:-19159799 | MS.gene069094:CDS |
| AAAGCTGCACTCAAGAGCAA+GGG | 0.647133 | 5.3:-19160009 | MS.gene069094:intron |
| ACAACACATTGCCCAAACCC+AGG | 0.649057 | 5.3:+19159815 | None:intergenic |
| TGTGCAAGACTGGTAAAGGT+TGG | 0.662647 | 5.3:-19159795 | MS.gene069094:CDS |
| AGTGAAACATTCTGCAAGTG+TGG | 0.671634 | 5.3:-19159139 | MS.gene069094:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAAATATAATTCATTTT+TGG | - | chr5.3:19159784-19159803 | MS.gene069094:CDS | 10.0% |
| !! | AAAACTATATAGATGAAATA+AGG | + | chr5.3:19159096-19159115 | None:intergenic | 15.0% |
| !!! | TTCATTTTTTAGATTGAATT+GGG | - | chr5.3:19159544-19159563 | MS.gene069094:intron | 15.0% |
| !!! | TTTCATTTTTTAGATTGAAT+TGG | - | chr5.3:19159543-19159562 | MS.gene069094:intron | 15.0% |
| !! | ATTAACTCTTGAGATAAAAT+CGG | - | chr5.3:19159714-19159733 | MS.gene069094:intron | 20.0% |
| !! | GATGGTAGTAAAATTAAAAT+AGG | + | chr5.3:19159387-19159406 | None:intergenic | 20.0% |
| !! | GATTTATTTATGCTTGAAAA+TGG | + | chr5.3:19159160-19159179 | None:intergenic | 20.0% |
| !! | GTAAAATTAAAATAGGCAAA+AGG | + | chr5.3:19159380-19159399 | None:intergenic | 20.0% |
| !!! | AGTTAATTCTGAAAAACAAT+AGG | + | chr5.3:19159701-19159720 | None:intergenic | 20.0% |
| !!! | TTTTTCAAAATCGAATGAAT+TGG | - | chr5.3:19159454-19159473 | MS.gene069094:intron | 20.0% |
| ! | TAAATATAAGCCTAGATTTG+TGG | + | chr5.3:19159765-19159784 | None:intergenic | 25.0% |
| ! | TGTTGTAACAATAGTTGAAT+CGG | + | chr5.3:19159652-19159671 | None:intergenic | 25.0% |
| ! | TTTGATCAAAATCATTTCGA+TGG | + | chr5.3:19159405-19159424 | None:intergenic | 25.0% |
| AATGAAACTGCAAAGTAACA+AGG | + | chr5.3:19159530-19159549 | None:intergenic | 30.0% | |
| AGATTTGTGGCATATTTGAA+TGG | + | chr5.3:19159752-19159771 | None:intergenic | 30.0% | |
| ATAGTTCAAGACAAAAACTC+AGG | - | chr5.3:19159214-19159233 | MS.gene069094:intron | 30.0% | |
| ATCTAGCAGTGCAATATATA+TGG | + | chr5.3:19159820-19159839 | None:intergenic | 30.0% | |
| TCAAAATCGAATGAATTGGT+TGG | - | chr5.3:19159458-19159477 | MS.gene069094:intron | 30.0% | |
| TTCAAATATGCCACAAATCT+AGG | - | chr5.3:19159752-19159771 | MS.gene069094:CDS | 30.0% | |
| ! | AAATGGAACATGAAGAGTAT+TGG | + | chr5.3:19159143-19159162 | None:intergenic | 30.0% |
| ! | GGCAAAGTTTTTGTTAAATG+CGG | - | chr5.3:19159316-19159335 | MS.gene069094:intron | 30.0% |
| AACAAGGATCAAATCGATTG+AGG | + | chr5.3:19159514-19159533 | None:intergenic | 35.0% | |
| GTTGGACATGTGTTATAACT+AGG | - | chr5.3:19159275-19159294 | MS.gene069094:intron | 35.0% | |
| TCTGAAAAACAATAGGACTC+TGG | + | chr5.3:19159694-19159713 | None:intergenic | 35.0% | |
| TGTGTTATAACTAGGACTAC+TGG | - | chr5.3:19159283-19159302 | MS.gene069094:intron | 35.0% | |
| ! | AATAGGACTCTGGTTCAAAT+CGG | + | chr5.3:19159684-19159703 | None:intergenic | 35.0% |
| ! | AATGAATTGGTTGGTTCAAC+TGG | - | chr5.3:19159467-19159486 | MS.gene069094:intron | 35.0% |
| ! | TAAACCAATAAGCTTTGTGC+AGG | - | chr5.3:19159185-19159204 | MS.gene069094:CDS | 35.0% |
| !! | GATGGAGTTGTTCCATTTTT+AGG | - | chr5.3:19159941-19159960 | MS.gene069094:intron | 35.0% |
| !!! | GCGCAACTAATTGTCATTTT+AGG | - | chr5.3:19159587-19159606 | MS.gene069094:intron | 35.0% |
| !!! | TGTTCCATTTTTAGGACTGA+AGG | - | chr5.3:19159949-19159968 | MS.gene069094:intron | 35.0% |
| ACAAAAACTTTGCCAGCATC+AGG | + | chr5.3:19159310-19159329 | None:intergenic | 40.0% | |
| AGTGAAACATTCTGCAAGTG+TGG | - | chr5.3:19159913-19159932 | MS.gene069094:intron | 40.0% | |
| ATTGAGAGTAGCAGTGACAA+TGG | - | chr5.3:19159889-19159908 | MS.gene069094:intron | 40.0% | |
| CTTTCCTGCACAAAGCTTAT+TGG | + | chr5.3:19159192-19159211 | None:intergenic | 40.0% | |
| TCACTGCTACTCTCAATTTC+AGG | + | chr5.3:19159886-19159905 | None:intergenic | 40.0% | |
| AAAAACTCAGGCCTGGGTTT+GGG | - | chr5.3:19159226-19159245 | MS.gene069094:intron | 45.0% | |
| AAAGCTGCACTCAAGAGCAA+GGG | - | chr5.3:19159043-19159062 | MS.gene069094:CDS | 45.0% | |
| ACATTCTGCAAGTGTGGTGA+AGG | - | chr5.3:19159919-19159938 | MS.gene069094:intron | 45.0% | |
| AGGGCCTTCAGTCCTAAAAA+TGG | + | chr5.3:19159956-19159975 | None:intergenic | 45.0% | |
| CAAGACAAAAACTCAGGCCT+GGG | - | chr5.3:19159220-19159239 | MS.gene069094:intron | 45.0% | |
| GTGTTGTGCAAGACTGGTAA+AGG | - | chr5.3:19159253-19159272 | MS.gene069094:intron | 45.0% | |
| TCAAGACAAAAACTCAGGCC+TGG | - | chr5.3:19159219-19159238 | MS.gene069094:intron | 45.0% | |
| TCTTGTGCATGCAGTGATGA+TGG | - | chr5.3:19159850-19159869 | MS.gene069094:intron | 45.0% | |
| TGTGCAAGACTGGTAAAGGT+TGG | - | chr5.3:19159257-19159276 | MS.gene069094:intron | 45.0% | |
| TTGCTCTTGAGTGCAGCTTT+GGG | + | chr5.3:19159043-19159062 | None:intergenic | 45.0% | |
| ACAACACATTGCCCAAACCC+AGG | + | chr5.3:19159240-19159259 | None:intergenic | 50.0% | |
| CAAAAACTCAGGCCTGGGTT+TGG | - | chr5.3:19159225-19159244 | MS.gene069094:intron | 50.0% | |
| CAAAGCTGCACTCAAGAGCA+AGG | - | chr5.3:19159042-19159061 | MS.gene069094:CDS | 50.0% | |
| CCTGATGCAGATTCAGGCAA+GGG | - | chr5.3:19159973-19159992 | MS.gene069094:intron | 50.0% | |
| CCTTGCCTGAATCTGCATCA+GGG | + | chr5.3:19159975-19159994 | None:intergenic | 50.0% | |
| CTTGCTCTTGAGTGCAGCTT+TGG | + | chr5.3:19159044-19159063 | None:intergenic | 50.0% | |
| GGCAATGTGTTGTGCAAGAC+TGG | - | chr5.3:19159247-19159266 | MS.gene069094:intron | 50.0% | |
| GTGTGTGACATACAGTGTGC+AGG | + | chr5.3:19159353-19159372 | None:intergenic | 50.0% | |
| TCTGCAAGTGTGGTGAAGGA+TGG | - | chr5.3:19159923-19159942 | MS.gene069094:intron | 50.0% | |
| AGGACTACTGGACCTGATGC+TGG | - | chr5.3:19159295-19159314 | MS.gene069094:intron | 55.0% | |
| CCCTGATGCAGATTCAGGCA+AGG | - | chr5.3:19159972-19159991 | MS.gene069094:intron | 55.0% | |
| CCCTTGCCTGAATCTGCATC+AGG | + | chr5.3:19159976-19159995 | None:intergenic | 55.0% | |
| GAAGGCCCTGATGCAGATTC+AGG | - | chr5.3:19159967-19159986 | MS.gene069094:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 19159033 | 19160041 | 19159033 | ID=MS.gene069094 |
| chr5.3 | mRNA | 19159033 | 19160041 | 19159033 | ID=MS.gene069094.t1;Parent=MS.gene069094 |
| chr5.3 | exon | 19160010 | 19160041 | 19160010 | ID=MS.gene069094.t1.exon1;Parent=MS.gene069094.t1 |
| chr5.3 | CDS | 19160010 | 19160041 | 19160010 | ID=cds.MS.gene069094.t1;Parent=MS.gene069094.t1 |
| chr5.3 | exon | 19159715 | 19159867 | 19159715 | ID=MS.gene069094.t1.exon2;Parent=MS.gene069094.t1 |
| chr5.3 | CDS | 19159715 | 19159867 | 19159715 | ID=cds.MS.gene069094.t1;Parent=MS.gene069094.t1 |
| chr5.3 | exon | 19159033 | 19159210 | 19159033 | ID=MS.gene069094.t1.exon3;Parent=MS.gene069094.t1 |
| chr5.3 | CDS | 19159033 | 19159210 | 19159033 | ID=cds.MS.gene069094.t1;Parent=MS.gene069094.t1 |
| Gene Sequence |
| Protein sequence |
