Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069271.t1 | XP_024633871.1 | 83.1 | 65 | 11 | 0 | 1 | 65 | 69 | 133 | 5.50E-20 | 106.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069271.t1 | G7KTR1 | 57.8 | 64 | 27 | 0 | 1 | 64 | 461 | 524 | 7.8e-08 | 65.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene069271 | 0.824213 | 9.12E-54 | -1.69E-46 |
| MS.gene049853 | MS.gene069271 | 0.858182 | 9.82E-63 | -1.69E-46 |
| MS.gene052447 | MS.gene069271 | 0.815598 | 8.52E-52 | -1.69E-46 |
| MS.gene054174 | MS.gene069271 | 0.840341 | 9.22E-58 | -1.69E-46 |
| MS.gene054175 | MS.gene069271 | 0.843377 | 1.46E-58 | -1.69E-46 |
| MS.gene055017 | MS.gene069271 | 0.804123 | 2.51E-49 | -1.69E-46 |
| MS.gene055058 | MS.gene069271 | 0.809649 | 1.70E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene069271 | 0.815417 | 9.34E-52 | -1.69E-46 |
| MS.gene058515 | MS.gene069271 | 0.800623 | 1.32E-48 | -1.69E-46 |
| MS.gene058580 | MS.gene069271 | 0.810174 | 1.31E-50 | -1.69E-46 |
| MS.gene059458 | MS.gene069271 | 0.810405 | 1.17E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069271.t1 | MTR_4g032565 | 58.065 | 62 | 26 | 0 | 4 | 65 | 224 | 285 | 5.88e-15 | 67.0 |
| MS.gene069271.t1 | MTR_4g032585 | 58.065 | 62 | 26 | 0 | 4 | 65 | 224 | 285 | 6.25e-15 | 66.6 |
| MS.gene069271.t1 | MTR_7g110100 | 57.812 | 64 | 27 | 0 | 1 | 64 | 461 | 524 | 8.45e-14 | 64.3 |
| MS.gene069271.t1 | MTR_3g452170 | 57.812 | 64 | 27 | 0 | 1 | 64 | 388 | 451 | 4.25e-13 | 62.0 |
| MS.gene069271.t1 | MTR_8g064300 | 50.794 | 63 | 31 | 0 | 2 | 64 | 247 | 309 | 4.78e-12 | 58.9 |
| MS.gene069271.t1 | MTR_5g093790 | 50.794 | 63 | 31 | 0 | 2 | 64 | 321 | 383 | 5.19e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGAAAGAGATCTGCTGATT+TGG | 0.291847 | 3.3:+7895759 | MS.gene069271:CDS |
| ACCTTGCTACACGGAGTAAT+CGG | 0.310589 | 3.3:-7895733 | None:intergenic |
| ATCTTTGTTGACATCAGTCT+TGG | 0.386824 | 3.3:-7895704 | None:intergenic |
| TTCACACATTTCAGCTTGGC+AGG | 0.413396 | 3.3:-7895835 | None:intergenic |
| AGATCGAAAAGGATGATTGA+TGG | 0.473069 | 3.3:+7895857 | MS.gene069271:CDS |
| ACTCCGTGTAGCAAGGTGTC+TGG | 0.492172 | 3.3:+7895739 | MS.gene069271:CDS |
| TTGGATGATTGTGATATTGT+CGG | 0.498738 | 3.3:+7895778 | MS.gene069271:CDS |
| CCTTGCTACACGGAGTAATC+GGG | 0.528739 | 3.3:-7895732 | None:intergenic |
| CCCGATTACTCCGTGTAGCA+AGG | 0.584861 | 3.3:+7895732 | MS.gene069271:CDS |
| GATCTTCACACATTTCAGCT+TGG | 0.600610 | 3.3:-7895839 | None:intergenic |
| TTTCCAGACACCTTGCTACA+CGG | 0.601622 | 3.3:-7895742 | None:intergenic |
| CAGGTACAATTGCTTGAGGA+AGG | 0.604139 | 3.3:+7895673 | None:intergenic |
| GCTCTAATGATGATGGTGAA+AGG | 0.620732 | 3.3:+7895800 | MS.gene069271:CDS |
| ATTGTCGGCTCTAATGATGA+TGG | 0.644857 | 3.3:+7895793 | MS.gene069271:CDS |
| TGTACAGGTACAATTGCTTG+AGG | 0.649475 | 3.3:+7895669 | None:intergenic |
| GAAATGTGTGAAGATCGAAA+AGG | 0.682154 | 3.3:+7895846 | MS.gene069271:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGGATGATTGTGATATTGT+CGG | + | chr3.3:7895778-7895797 | MS.gene069271:CDS | 30.0% | |
| ATCTTTGTTGACATCAGTCT+TGG | - | chr3.3:7895707-7895726 | None:intergenic | 35.0% | |
| GAAATGTGTGAAGATCGAAA+AGG | + | chr3.3:7895846-7895865 | MS.gene069271:CDS | 35.0% | |
| GATCTTCACACATTTCAGCT+TGG | - | chr3.3:7895842-7895861 | None:intergenic | 40.0% | |
| ! | TGGAAAGAGATCTGCTGATT+TGG | + | chr3.3:7895759-7895778 | MS.gene069271:CDS | 40.0% |
| !! | ATTGTCGGCTCTAATGATGA+TGG | + | chr3.3:7895793-7895812 | MS.gene069271:CDS | 40.0% |
| !! | GCTCTAATGATGATGGTGAA+AGG | + | chr3.3:7895800-7895819 | MS.gene069271:CDS | 40.0% |
| ACCTTGCTACACGGAGTAAT+CGG | - | chr3.3:7895736-7895755 | None:intergenic | 45.0% | |
| TTCACACATTTCAGCTTGGC+AGG | - | chr3.3:7895838-7895857 | None:intergenic | 45.0% | |
| TTTCCAGACACCTTGCTACA+CGG | - | chr3.3:7895745-7895764 | None:intergenic | 45.0% | |
| CCTTGCTACACGGAGTAATC+GGG | - | chr3.3:7895735-7895754 | None:intergenic | 50.0% | |
| CCCGATTACTCCGTGTAGCA+AGG | + | chr3.3:7895732-7895751 | MS.gene069271:CDS | 55.0% | |
| ! | ACTCCGTGTAGCAAGGTGTC+TGG | + | chr3.3:7895739-7895758 | MS.gene069271:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 7895676 | 7895876 | 7895676 | ID=MS.gene069271 |
| chr3.3 | mRNA | 7895676 | 7895876 | 7895676 | ID=MS.gene069271.t1;Parent=MS.gene069271 |
| chr3.3 | exon | 7895676 | 7895876 | 7895676 | ID=MS.gene069271.t1.exon1;Parent=MS.gene069271.t1 |
| chr3.3 | CDS | 7895676 | 7895876 | 7895676 | ID=cds.MS.gene069271.t1;Parent=MS.gene069271.t1 |
| Gene Sequence |
| Protein sequence |
