Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06949.t1 | XP_003602219.1 | 96.8 | 593 | 18 | 1 | 1 | 593 | 1 | 592 | 2.40E-300 | 1040.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06949.t1 | G7J6T8 | 96.8 | 593 | 18 | 1 | 1 | 593 | 1 | 592 | 1.7e-300 | 1040.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049807 | MS.gene06949 | 0.81782 | 2.70E-52 | -1.69E-46 |
| MS.gene050359 | MS.gene06949 | 0.816249 | 6.10E-52 | -1.69E-46 |
| MS.gene050414 | MS.gene06949 | 0.805563 | 1.26E-49 | -1.69E-46 |
| MS.gene050551 | MS.gene06949 | 0.800447 | 1.44E-48 | -1.69E-46 |
| MS.gene051852 | MS.gene06949 | 0.825116 | 5.59E-54 | -1.69E-46 |
| MS.gene05224 | MS.gene06949 | 0.807869 | 4.09E-50 | -1.69E-46 |
| MS.gene052296 | MS.gene06949 | 0.806942 | 6.44E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene06949 | 0.81594 | 7.14E-52 | -1.69E-46 |
| MS.gene052463 | MS.gene06949 | 0.837929 | 3.89E-57 | -1.69E-46 |
| MS.gene055465 | MS.gene06949 | 0.815417 | 9.34E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene06949 | 0.83108 | 2.04E-55 | -1.69E-46 |
| MS.gene055513 | MS.gene06949 | 0.806151 | 9.46E-50 | -1.69E-46 |
| MS.gene055864 | MS.gene06949 | 0.824941 | 6.14E-54 | -1.69E-46 |
| MS.gene056285 | MS.gene06949 | 0.825718 | 4.02E-54 | -1.69E-46 |
| MS.gene058720 | MS.gene06949 | 0.806268 | 8.93E-50 | -1.69E-46 |
| MS.gene059170 | MS.gene06949 | 0.822894 | 1.86E-53 | -1.69E-46 |
| MS.gene060932 | MS.gene06949 | 0.806336 | 8.64E-50 | -1.69E-46 |
| MS.gene061006 | MS.gene06949 | 0.801356 | 9.36E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene06949 | 0.829764 | 4.29E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06949.t1 | MTR_3g091180 | 97.639 | 593 | 13 | 1 | 1 | 593 | 1 | 592 | 0.0 | 1202 |
| MS.gene06949.t1 | MTR_1g092540 | 64.901 | 604 | 192 | 7 | 1 | 592 | 1 | 596 | 0.0 | 776 |
| MS.gene06949.t1 | MTR_1g044135 | 37.763 | 617 | 320 | 18 | 1 | 581 | 1 | 589 | 1.36e-118 | 365 |
| MS.gene06949.t1 | MTR_1g061540 | 39.691 | 582 | 268 | 15 | 5 | 570 | 9 | 523 | 2.48e-113 | 348 |
| MS.gene06949.t1 | MTR_7g103490 | 37.767 | 609 | 297 | 10 | 1 | 586 | 5 | 554 | 1.78e-110 | 342 |
| MS.gene06949.t1 | MTR_0318s0020 | 50.431 | 232 | 94 | 4 | 364 | 582 | 343 | 566 | 7.75e-66 | 225 |
| MS.gene06949.t1 | MTR_0318s0020 | 35.573 | 253 | 144 | 3 | 15 | 248 | 117 | 369 | 8.29e-43 | 162 |
| MS.gene06949.t1 | MTR_1g073890 | 61.635 | 159 | 56 | 2 | 423 | 581 | 406 | 559 | 4.64e-59 | 207 |
| MS.gene06949.t1 | MTR_1g073890 | 35.659 | 258 | 143 | 5 | 1 | 237 | 1 | 256 | 1.74e-44 | 167 |
| MS.gene06949.t1 | MTR_6g461910 | 55.357 | 168 | 70 | 2 | 413 | 579 | 342 | 505 | 1.44e-51 | 185 |
| MS.gene06949.t1 | MTR_6g461910 | 41.589 | 214 | 123 | 2 | 19 | 230 | 29 | 242 | 1.38e-45 | 169 |
| MS.gene06949.t1 | MTR_4g021260 | 41.549 | 142 | 83 | 0 | 423 | 564 | 331 | 472 | 4.04e-31 | 127 |
| MS.gene06949.t1 | MTR_4g021260 | 27.485 | 342 | 202 | 9 | 15 | 327 | 8 | 332 | 3.95e-30 | 124 |
| MS.gene06949.t1 | MTR_7g063040 | 39.735 | 151 | 84 | 2 | 422 | 567 | 331 | 479 | 2.80e-27 | 115 |
| MS.gene06949.t1 | MTR_7g063040 | 22.936 | 327 | 219 | 4 | 21 | 327 | 20 | 333 | 1.80e-25 | 110 |
| MS.gene06949.t1 | MTR_1g096970 | 27.570 | 214 | 137 | 3 | 26 | 229 | 51 | 256 | 7.34e-16 | 81.3 |
| MS.gene06949.t1 | MTR_1g096970 | 29.747 | 158 | 94 | 2 | 426 | 573 | 270 | 420 | 1.25e-13 | 73.9 |
| MS.gene06949.t1 | MTR_3g114010 | 28.774 | 212 | 137 | 5 | 26 | 229 | 122 | 327 | 7.76e-14 | 75.1 |
| MS.gene06949.t1 | MTR_3g114010 | 32.237 | 152 | 86 | 3 | 424 | 565 | 340 | 484 | 3.13e-13 | 73.2 |
| MS.gene06949.t1 | MTR_3g097240 | 25.980 | 204 | 148 | 1 | 26 | 229 | 141 | 341 | 2.37e-13 | 72.8 |
| MS.gene06949.t1 | MTR_3g097240 | 25.980 | 204 | 148 | 1 | 26 | 229 | 141 | 341 | 2.86e-13 | 72.4 |
| MS.gene06949.t1 | MTR_3g097240 | 25.980 | 204 | 148 | 1 | 26 | 229 | 141 | 341 | 5.11e-13 | 72.4 |
| MS.gene06949.t1 | MTR_1g025340 | 30.573 | 157 | 91 | 3 | 424 | 569 | 345 | 494 | 1.30e-12 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06949.t1 | AT5G04550 | 55.316 | 602 | 255 | 6 | 1 | 592 | 1 | 598 | 0.0 | 627 |
| MS.gene06949.t1 | AT5G04550 | 55.316 | 602 | 255 | 6 | 1 | 592 | 1 | 598 | 0.0 | 627 |
| MS.gene06949.t1 | AT3G23160 | 60.510 | 157 | 56 | 3 | 426 | 581 | 360 | 511 | 1.21e-55 | 197 |
| MS.gene06949.t1 | AT3G23160 | 41.063 | 207 | 121 | 1 | 23 | 229 | 40 | 245 | 1.42e-48 | 177 |
| MS.gene06949.t1 | AT5G51670 | 55.758 | 165 | 60 | 3 | 418 | 576 | 306 | 463 | 1.96e-51 | 184 |
| MS.gene06949.t1 | AT5G51670 | 35.349 | 215 | 127 | 1 | 26 | 228 | 37 | 251 | 8.30e-41 | 154 |
| MS.gene06949.t1 | AT5G51670 | 55.758 | 165 | 60 | 3 | 418 | 576 | 306 | 463 | 1.96e-51 | 184 |
| MS.gene06949.t1 | AT5G51670 | 35.349 | 215 | 127 | 1 | 26 | 228 | 37 | 251 | 8.30e-41 | 154 |
| MS.gene06949.t1 | AT5G08660 | 33.333 | 165 | 85 | 5 | 427 | 580 | 372 | 522 | 1.45e-14 | 77.4 |
| MS.gene06949.t1 | AT5G08660 | 27.103 | 214 | 138 | 3 | 26 | 229 | 149 | 354 | 7.06e-11 | 65.5 |
| MS.gene06949.t1 | AT5G08660 | 33.333 | 165 | 85 | 5 | 427 | 580 | 372 | 522 | 1.45e-14 | 77.4 |
| MS.gene06949.t1 | AT5G08660 | 27.103 | 214 | 138 | 3 | 26 | 229 | 149 | 354 | 7.06e-11 | 65.5 |
| MS.gene06949.t1 | AT5G08660 | 33.333 | 165 | 85 | 5 | 427 | 580 | 372 | 522 | 1.45e-14 | 77.4 |
| MS.gene06949.t1 | AT5G08660 | 27.103 | 214 | 138 | 3 | 26 | 229 | 149 | 354 | 7.06e-11 | 65.5 |
| MS.gene06949.t1 | AT1G34320 | 26.389 | 216 | 139 | 3 | 26 | 229 | 155 | 362 | 5.07e-14 | 75.9 |
| MS.gene06949.t1 | AT1G34320 | 29.697 | 165 | 95 | 4 | 418 | 569 | 368 | 524 | 4.53e-13 | 72.8 |
| MS.gene06949.t1 | AT1G34320 | 26.389 | 216 | 139 | 3 | 26 | 229 | 155 | 362 | 5.07e-14 | 75.9 |
| MS.gene06949.t1 | AT1G34320 | 29.697 | 165 | 95 | 4 | 418 | 569 | 368 | 524 | 4.53e-13 | 72.8 |
| MS.gene06949.t1 | AT5G08660 | 27.103 | 214 | 138 | 3 | 26 | 229 | 149 | 354 | 3.82e-11 | 66.2 |
Find 123 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTATGCTTTCCTAAAAT+TGG | 0.164702 | 3.1:-73177099 | None:intergenic |
| TGTCCAAGCTGGTTAATTTA+TGG | 0.173391 | 3.1:+73176272 | MS.gene06949:CDS |
| CTGAATTATGTCTGCAAATA+TGG | 0.209847 | 3.1:+73177843 | MS.gene06949:CDS |
| GCAGCTGCACCAAGGGTTTC+AGG | 0.229194 | 3.1:-73177432 | None:intergenic |
| GTTTAAGACACTCGAGTTAT+TGG | 0.231922 | 3.1:-73176878 | None:intergenic |
| AATGCAAGTGGCGTTCATTA+TGG | 0.240646 | 3.1:+73177336 | MS.gene06949:CDS |
| AAACACTCTTCCGCCATAAC+TGG | 0.252428 | 3.1:+73177216 | MS.gene06949:CDS |
| ATCTAGCAGTAATAGTATTA+AGG | 0.255713 | 3.1:-73176999 | None:intergenic |
| CTTAGAGAATATTGTGAATA+AGG | 0.272293 | 3.1:-73176809 | None:intergenic |
| GTCTTTGACACAAGTCTTGC+AGG | 0.283346 | 3.1:+73177627 | MS.gene06949:CDS |
| TGATTACACGATACATCTTT+TGG | 0.284499 | 3.1:+73176780 | MS.gene06949:CDS |
| AAAGCCAGCATTTCTCATTC+AGG | 0.310641 | 3.1:+73177045 | MS.gene06949:CDS |
| CGTAGTGATAAACCCATAAA+AGG | 0.312698 | 3.1:-73176500 | None:intergenic |
| TATGGAGATCGTTGAGAATA+TGG | 0.317599 | 3.1:+73176405 | MS.gene06949:CDS |
| GCTTTATTTGTTCTAACAAT+TGG | 0.326268 | 3.1:-73177027 | None:intergenic |
| TTCTCAAAACCTTTCAAAAT+CGG | 0.332274 | 3.1:-73176475 | None:intergenic |
| CTTCGTATATGGCTTCAATT+TGG | 0.333531 | 3.1:-73177586 | None:intergenic |
| GATGCCAAGGTCTTCGTATA+TGG | 0.340969 | 3.1:-73177597 | None:intergenic |
| TGGCTTCAATTTGGCCTTGA+GGG | 0.346452 | 3.1:-73177577 | None:intergenic |
| TGAGTATGATGAACTACTTT+TGG | 0.346467 | 3.1:-73177369 | None:intergenic |
| ATGCAAGTGGCGTTCATTAT+GGG | 0.351200 | 3.1:+73177337 | MS.gene06949:CDS |
| TACCAGCAGCACATTTGTTC+GGG | 0.351505 | 3.1:-73177757 | None:intergenic |
| GCGCCATCCGACTCATTATT+AGG | 0.353064 | 3.1:-73176670 | None:intergenic |
| CAAGTTAATTCCCACCCATA+TGG | 0.362762 | 3.1:-73176532 | None:intergenic |
| TTGTATGCTTTCCTAAAATT+GGG | 0.365181 | 3.1:-73177098 | None:intergenic |
| CGAAGCAATGTCAAGCATAT+TGG | 0.366671 | 3.1:+73177659 | MS.gene06949:CDS |
| GTGAGTTTCCTTCCCAGTTA+TGG | 0.383182 | 3.1:-73177229 | None:intergenic |
| GTACCAGCAGCACATTTGTT+CGG | 0.401978 | 3.1:-73177758 | None:intergenic |
| TTCTTGATTTGCAAAGTAAA+GGG | 0.402434 | 3.1:-73177784 | None:intergenic |
| TTTCTTGATTTGCAAAGTAA+AGG | 0.404302 | 3.1:-73177785 | None:intergenic |
| CATTGAACGATTGATCTGTA+TGG | 0.406973 | 3.1:+73176387 | MS.gene06949:CDS |
| GATGGAGGTGCTTGTTGATC+TGG | 0.409544 | 3.1:+73176624 | MS.gene06949:CDS |
| GAGGGCAATTCTCACACGTC+TGG | 0.409757 | 3.1:-73177559 | None:intergenic |
| AAGGGGTCCTGAAGAAAATC+TGG | 0.414678 | 3.1:-73176980 | None:intergenic |
| TGGACCTTTCAAAGGATGTA+TGG | 0.419035 | 3.1:+73177278 | MS.gene06949:CDS |
| TCTAGCAGTAATAGTATTAA+GGG | 0.420518 | 3.1:-73176998 | None:intergenic |
| GGTGACTCATCCACAAAATC+AGG | 0.427673 | 3.1:+73177075 | MS.gene06949:CDS |
| ATTTCTCATTCAGGTCCTCT+TGG | 0.429289 | 3.1:+73177054 | MS.gene06949:CDS |
| CACCAAGGGTTTCAGGTGGA+GGG | 0.429974 | 3.1:-73177425 | None:intergenic |
| ACACAAGTCTTGCAGGAGAA+TGG | 0.437247 | 3.1:+73177634 | MS.gene06949:CDS |
| GAGGAGATCACAAATTCAAT+TGG | 0.437515 | 3.1:+73176331 | MS.gene06949:CDS |
| GGGATGTTTCTCTGTGGAAC+AGG | 0.445839 | 3.1:+73176752 | MS.gene06949:CDS |
| AGAAGAAAGTTGCATGGAAA+AGG | 0.452080 | 3.1:+73176710 | MS.gene06949:CDS |
| GCACCAAGGGTTTCAGGTGG+AGG | 0.459060 | 3.1:-73177426 | None:intergenic |
| AAGCAATGGTTGCGGAATCA+TGG | 0.461314 | 3.1:+73176158 | None:intergenic |
| AATTCTGAGAAGGAGGTGAT+TGG | 0.466034 | 3.1:+73176220 | MS.gene06949:CDS |
| GTCAAGCATATTGGAATGGT+TGG | 0.470687 | 3.1:+73177668 | MS.gene06949:CDS |
| GAACGCCACTTGCATTTGAA+TGG | 0.471089 | 3.1:-73177329 | None:intergenic |
| CTTTCAAAGGATGTATGGCT+TGG | 0.472012 | 3.1:+73177283 | MS.gene06949:CDS |
| GGAGCTCAATGCAAAATCTC+TGG | 0.491321 | 3.1:+73177869 | MS.gene06949:CDS |
| TTTCAAAGGATGTATGGCTT+GGG | 0.497824 | 3.1:+73177284 | MS.gene06949:CDS |
| GCATGAGGTGAAGAACTTGC+GGG | 0.500274 | 3.1:+73176732 | MS.gene06949:CDS |
| ACTACGGGTACAGATCCATA+TGG | 0.502018 | 3.1:+73176517 | MS.gene06949:CDS |
| AGGACCTGAATGAGAAATGC+TGG | 0.505217 | 3.1:-73177049 | None:intergenic |
| AACCTTTCAAAATCGGATCA+TGG | 0.507399 | 3.1:-73176468 | None:intergenic |
| TTGATTCATGTTCCTTCCAA+GGG | 0.512029 | 3.1:-73177142 | None:intergenic |
| ATTGATTCATGTTCCTTCCA+AGG | 0.513777 | 3.1:-73177143 | None:intergenic |
| AATACTATTACTGCTAGATC+AGG | 0.517795 | 3.1:+73177003 | MS.gene06949:CDS |
| ATTATGTCTGCAAATATGGC+AGG | 0.518317 | 3.1:+73177847 | MS.gene06949:CDS |
| TTGCCATAAATTAACCAGCT+TGG | 0.526727 | 3.1:-73176275 | None:intergenic |
| TTTACTCAGGTCCCCTTGGA+AGG | 0.533412 | 3.1:+73177130 | MS.gene06949:CDS |
| GTAGTCTGTGGAGGACTCCT+CGG | 0.540847 | 3.1:+73176185 | MS.gene06949:CDS |
| AACACTCTTCCGCCATAACT+GGG | 0.544435 | 3.1:+73177217 | MS.gene06949:CDS |
| ACTCAAGTTGGACCTTTCAA+AGG | 0.547610 | 3.1:+73177270 | MS.gene06949:CDS |
| AGGGCAATTCTCACACGTCT+GGG | 0.550322 | 3.1:-73177558 | None:intergenic |
| AACCCTCCACCTGAAACCCT+TGG | 0.551082 | 3.1:+73177423 | MS.gene06949:CDS |
| TGAGTGATAAGCATATATCT+AGG | 0.551088 | 3.1:+73176302 | MS.gene06949:CDS |
| ATGTGGCCGAGTCCGTGGCT+AGG | 0.552509 | 3.1:+73176431 | MS.gene06949:CDS |
| TTCCCGAACAAATGTGCTGC+TGG | 0.552642 | 3.1:+73177755 | MS.gene06949:CDS |
| CATAGTCGAATGACTCAAGT+TGG | 0.553493 | 3.1:+73177258 | MS.gene06949:CDS |
| ACGTGTGAGAATTGCCCTCA+AGG | 0.554129 | 3.1:+73177563 | MS.gene06949:CDS |
| GTCTGGGTAACATGTTGTAC+AGG | 0.554326 | 3.1:-73177542 | None:intergenic |
| AGCAAGCTTGATGTCCAAGC+TGG | 0.554952 | 3.1:+73176261 | MS.gene06949:CDS |
| ATAATCACCGAGCTTCTTGT+GGG | 0.555663 | 3.1:+73177819 | MS.gene06949:CDS |
| TATTATTATTCTGTGATGAG+TGG | 0.557224 | 3.1:-73176954 | None:intergenic |
| AATAATCACCGAGCTTCTTG+TGG | 0.558759 | 3.1:+73177818 | MS.gene06949:CDS |
| GGCACATGTGGCCGAGTCCG+TGG | 0.569052 | 3.1:+73176426 | MS.gene06949:CDS |
| GCAGCTTCTCCACACTTGAT+CGG | 0.571839 | 3.1:+73177501 | MS.gene06949:CDS |
| GCATATATCTAGGTTGAAAG+AGG | 0.572414 | 3.1:+73176312 | MS.gene06949:CDS |
| ATGGCTTCAATTTGGCCTTG+AGG | 0.575637 | 3.1:-73177578 | None:intergenic |
| TGCAAGTCTGTATCAAGAGA+TGG | 0.582369 | 3.1:+73176606 | MS.gene06949:CDS |
| TGAAAGCCTAGCCACGGACT+CGG | 0.586035 | 3.1:-73176437 | None:intergenic |
| GGCATGAGGTGAAGAACTTG+CGG | 0.586790 | 3.1:+73176731 | MS.gene06949:CDS |
| GACTGCCATTCAAATGCAAG+TGG | 0.587500 | 3.1:+73177324 | MS.gene06949:CDS |
| AATCATGGTTTCGTAGTCTG+TGG | 0.589487 | 3.1:+73176173 | MS.gene06949:CDS |
| CGTTGAGAATATGGCACATG+TGG | 0.590918 | 3.1:+73176414 | MS.gene06949:CDS |
| TCTCTTGAATCCCGAAAACA+TGG | 0.590944 | 3.1:-73176837 | None:intergenic |
| CTCTTCCGCCATAACTGGGA+AGG | 0.593341 | 3.1:+73177221 | MS.gene06949:CDS |
| CAATGTCAAGCATATTGGAA+TGG | 0.593748 | 3.1:+73177664 | MS.gene06949:CDS |
| ATTCAGACCCACAAGAAGCT+CGG | 0.597225 | 3.1:-73177826 | None:intergenic |
| CGTATCATGTTATGAGCAAG+CGG | 0.599166 | 3.1:-73177693 | None:intergenic |
| CAAGAGATGGTAGATGATGG+TGG | 0.602012 | 3.1:+73176853 | MS.gene06949:CDS |
| ATTCAAGAGATGGTAGATGA+TGG | 0.602934 | 3.1:+73176850 | MS.gene06949:CDS |
| TGATAAAGCAGCTGCACCAA+GGG | 0.605605 | 3.1:-73177439 | None:intergenic |
| GCATGATGCTAATTCTGAGA+AGG | 0.608544 | 3.1:+73176210 | MS.gene06949:CDS |
| AGTATCAGAAGAAAGTTGCA+TGG | 0.611702 | 3.1:+73176704 | MS.gene06949:CDS |
| CAAGCCATACATCCTTTGAA+AGG | 0.613969 | 3.1:-73177282 | None:intergenic |
| TCCTAGATAAGCAATGGTTG+CGG | 0.617445 | 3.1:+73176150 | None:intergenic |
| GTGATAAAGCAGCTGCACCA+AGG | 0.620790 | 3.1:-73177440 | None:intergenic |
| TTTCTTTGAAAGCCTAGCCA+CGG | 0.624883 | 3.1:-73176443 | None:intergenic |
| AGTGAAGCCTAATAATGAGT+CGG | 0.624980 | 3.1:+73176663 | MS.gene06949:CDS |
| AAAGACTGATGACGATGCCA+AGG | 0.625890 | 3.1:-73177610 | None:intergenic |
| CGGGTACAGATCCATATGGG+TGG | 0.627773 | 3.1:+73176521 | MS.gene06949:CDS |
| CTACGGGTACAGATCCATAT+GGG | 0.628821 | 3.1:+73176518 | MS.gene06949:CDS |
| GCAAAATCTCTGGCAGAGTG+TGG | 0.631334 | 3.1:+73177879 | MS.gene06949:CDS |
| AAGCCTAATAATGAGTCGGA+TGG | 0.631568 | 3.1:+73176667 | MS.gene06949:CDS |
| AGTTTCCTTCCCAGTTATGG+CGG | 0.632458 | 3.1:-73177226 | None:intergenic |
| GAATTAGCATCATGCTTCCG+AGG | 0.632742 | 3.1:-73176202 | None:intergenic |
| GCGTCGAGACCGATCAAGTG+TGG | 0.634080 | 3.1:-73177510 | None:intergenic |
| GCTGCACCAAGGGTTTCAGG+TGG | 0.635184 | 3.1:-73177429 | None:intergenic |
| GAAGCCATATACGAAGACCT+TGG | 0.636162 | 3.1:+73177593 | MS.gene06949:CDS |
| TGATGCTAATTCTGAGAAGG+AGG | 0.639412 | 3.1:+73176213 | MS.gene06949:CDS |
| GGGTACAGATCCATATGGGT+GGG | 0.644087 | 3.1:+73176522 | MS.gene06949:CDS |
| CATGGTTTCGTAGTCTGTGG+AGG | 0.648414 | 3.1:+73176176 | MS.gene06949:CDS |
| TTATGTCTGCAAATATGGCA+GGG | 0.650727 | 3.1:+73177848 | MS.gene06949:CDS |
| TGATTCATGTTCCTTCCAAG+GGG | 0.662062 | 3.1:-73177141 | None:intergenic |
| CTAGCAGTAATAGTATTAAG+GGG | 0.675941 | 3.1:-73176997 | None:intergenic |
| AAGTCTGTATCAAGAGATGG+AGG | 0.682935 | 3.1:+73176609 | MS.gene06949:CDS |
| TTTGTGGATGAGTCACCAAG+AGG | 0.684677 | 3.1:-73177069 | None:intergenic |
| TTGCTCATAACATGATACGA+TGG | 0.690679 | 3.1:+73177697 | MS.gene06949:CDS |
| ACTTGCGGGATGTTTCTCTG+TGG | 0.694225 | 3.1:+73176746 | MS.gene06949:CDS |
| AGTTGCATGGAAAAGGCATG+AGG | 0.701910 | 3.1:+73176717 | MS.gene06949:CDS |
| TGAATGGCAGTCAATGACAG+AGG | 0.711575 | 3.1:-73177313 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCTAGCAGTAATAGTATTA+AGG | - | chr3.1:73177002-73177021 | None:intergenic | 25.0% |
| ! | CAAAAAAAATGAGCAAGATT+TGG | + | chr3.1:73177184-73177203 | MS.gene06949:CDS | 25.0% |
| ! | CTTAGAGAATATTGTGAATA+AGG | - | chr3.1:73176812-73176831 | None:intergenic | 25.0% |
| ! | TATTATTATTCTGTGATGAG+TGG | - | chr3.1:73176957-73176976 | None:intergenic | 25.0% |
| ! | TCTAGCAGTAATAGTATTAA+GGG | - | chr3.1:73177001-73177020 | None:intergenic | 25.0% |
| ! | TTCTCAAAACCTTTCAAAAT+CGG | - | chr3.1:73176478-73176497 | None:intergenic | 25.0% |
| ! | TTGTATGCTTTCCTAAAATT+GGG | - | chr3.1:73177101-73177120 | None:intergenic | 25.0% |
| ! | TTTGTATGCTTTCCTAAAAT+TGG | - | chr3.1:73177102-73177121 | None:intergenic | 25.0% |
| !! | ACAAATGTCAATTTTTACTC+AGG | + | chr3.1:73177117-73177136 | MS.gene06949:CDS | 25.0% |
| !!! | ATTTTTTTTGTTCTAGTGAG+TGG | - | chr3.1:73177174-73177193 | None:intergenic | 25.0% |
| !!! | GCTTTATTTGTTCTAACAAT+TGG | - | chr3.1:73177030-73177049 | None:intergenic | 25.0% |
| !!! | TTCTTGATTTGCAAAGTAAA+GGG | - | chr3.1:73177787-73177806 | None:intergenic | 25.0% |
| !!! | TTTCTTGATTTGCAAAGTAA+AGG | - | chr3.1:73177788-73177807 | None:intergenic | 25.0% |
| !!! | TTTTTTTTGTTCTAGTGAGT+GGG | - | chr3.1:73177173-73177192 | None:intergenic | 25.0% |
| AATACTATTACTGCTAGATC+AGG | + | chr3.1:73177003-73177022 | MS.gene06949:CDS | 30.0% | |
| CTAGCAGTAATAGTATTAAG+GGG | - | chr3.1:73177000-73177019 | None:intergenic | 30.0% | |
| CTGAATTATGTCTGCAAATA+TGG | + | chr3.1:73177843-73177862 | MS.gene06949:CDS | 30.0% | |
| TGAGTGATAAGCATATATCT+AGG | + | chr3.1:73176302-73176321 | MS.gene06949:CDS | 30.0% | |
| ! | AATATTGCCAGATTTTCTTC+AGG | + | chr3.1:73176973-73176992 | MS.gene06949:CDS | 30.0% |
| ! | CTTTTATGGGTTTATCACTA+CGG | + | chr3.1:73176501-73176520 | MS.gene06949:CDS | 30.0% |
| ! | TGAGTATGATGAACTACTTT+TGG | - | chr3.1:73177372-73177391 | None:intergenic | 30.0% |
| ! | TGATTACACGATACATCTTT+TGG | + | chr3.1:73176780-73176799 | MS.gene06949:CDS | 30.0% |
| ! | TTTTATGGGTTTATCACTAC+GGG | + | chr3.1:73176502-73176521 | MS.gene06949:CDS | 30.0% |
| !! | GGAATTAACTTGCAAAAAGA+TGG | + | chr3.1:73176543-73176562 | MS.gene06949:CDS | 30.0% |
| !! | TCTAAGATCAACCATGTTTT+CGG | + | chr3.1:73176826-73176845 | MS.gene06949:CDS | 30.0% |
| !!! | GTTTTGAGAACACCTTTTAT+GGG | + | chr3.1:73176488-73176507 | MS.gene06949:CDS | 30.0% |
| AACCTTTCAAAATCGGATCA+TGG | - | chr3.1:73176471-73176490 | None:intergenic | 35.0% | |
| AGAAGAAAGTTGCATGGAAA+AGG | + | chr3.1:73176710-73176729 | MS.gene06949:CDS | 35.0% | |
| AGTATCAGAAGAAAGTTGCA+TGG | + | chr3.1:73176704-73176723 | MS.gene06949:CDS | 35.0% | |
| AGTGAAGCCTAATAATGAGT+CGG | + | chr3.1:73176663-73176682 | MS.gene06949:CDS | 35.0% | |
| ATTATGTCTGCAAATATGGC+AGG | + | chr3.1:73177847-73177866 | MS.gene06949:CDS | 35.0% | |
| ATTCAAGAGATGGTAGATGA+TGG | + | chr3.1:73176850-73176869 | MS.gene06949:CDS | 35.0% | |
| CAATGTCAAGCATATTGGAA+TGG | + | chr3.1:73177664-73177683 | MS.gene06949:CDS | 35.0% | |
| CATTGAACGATTGATCTGTA+TGG | + | chr3.1:73176387-73176406 | MS.gene06949:CDS | 35.0% | |
| CGTAGTGATAAACCCATAAA+AGG | - | chr3.1:73176503-73176522 | None:intergenic | 35.0% | |
| CTTCGTATATGGCTTCAATT+TGG | - | chr3.1:73177589-73177608 | None:intergenic | 35.0% | |
| GAGGAGATCACAAATTCAAT+TGG | + | chr3.1:73176331-73176350 | MS.gene06949:CDS | 35.0% | |
| TATGGAGATCGTTGAGAATA+TGG | + | chr3.1:73176405-73176424 | MS.gene06949:CDS | 35.0% | |
| TGTCCAAGCTGGTTAATTTA+TGG | + | chr3.1:73176272-73176291 | MS.gene06949:CDS | 35.0% | |
| TTATGTCTGCAAATATGGCA+GGG | + | chr3.1:73177848-73177867 | MS.gene06949:CDS | 35.0% | |
| TTGATTCATGTTCCTTCCAA+GGG | - | chr3.1:73177145-73177164 | None:intergenic | 35.0% | |
| TTGCCATAAATTAACCAGCT+TGG | - | chr3.1:73176278-73176297 | None:intergenic | 35.0% | |
| TTGCTCATAACATGATACGA+TGG | + | chr3.1:73177697-73177716 | MS.gene06949:CDS | 35.0% | |
| TTTCAAAGGATGTATGGCTT+GGG | + | chr3.1:73177284-73177303 | MS.gene06949:CDS | 35.0% | |
| ! | ACAAAATCAGGCCCAATTTT+AGG | + | chr3.1:73177087-73177106 | MS.gene06949:CDS | 35.0% |
| ! | ATTGATTCATGTTCCTTCCA+AGG | - | chr3.1:73177146-73177165 | None:intergenic | 35.0% |
| ! | CTAAGATCAACCATGTTTTC+GGG | + | chr3.1:73176827-73176846 | MS.gene06949:CDS | 35.0% |
| ! | GCATATATCTAGGTTGAAAG+AGG | + | chr3.1:73176312-73176331 | MS.gene06949:CDS | 35.0% |
| ! | GTTTAAGACACTCGAGTTAT+TGG | - | chr3.1:73176881-73176900 | None:intergenic | 35.0% |
| ! | TAAAATTGGGCCTGATTTTG+TGG | - | chr3.1:73177088-73177107 | None:intergenic | 35.0% |
| !!! | GGTTTTGAGAACACCTTTTA+TGG | + | chr3.1:73176487-73176506 | MS.gene06949:CDS | 35.0% |
| AAAGCCAGCATTTCTCATTC+AGG | + | chr3.1:73177045-73177064 | MS.gene06949:CDS | 40.0% | |
| AAGCCTAATAATGAGTCGGA+TGG | + | chr3.1:73176667-73176686 | MS.gene06949:CDS | 40.0% | |
| AAGTCTGTATCAAGAGATGG+AGG | + | chr3.1:73176609-73176628 | MS.gene06949:CDS | 40.0% | |
| AATAATCACCGAGCTTCTTG+TGG | + | chr3.1:73177818-73177837 | MS.gene06949:CDS | 40.0% | |
| AATCATGGTTTCGTAGTCTG+TGG | + | chr3.1:73176173-73176192 | MS.gene06949:CDS | 40.0% | |
| AATGCAAGTGGCGTTCATTA+TGG | + | chr3.1:73177336-73177355 | MS.gene06949:CDS | 40.0% | |
| ATAATCACCGAGCTTCTTGT+GGG | + | chr3.1:73177819-73177838 | MS.gene06949:CDS | 40.0% | |
| ATGCAAGTGGCGTTCATTAT+GGG | + | chr3.1:73177337-73177356 | MS.gene06949:CDS | 40.0% | |
| ATTTCTCATTCAGGTCCTCT+TGG | + | chr3.1:73177054-73177073 | MS.gene06949:CDS | 40.0% | |
| CAAGCCATACATCCTTTGAA+AGG | - | chr3.1:73177285-73177304 | None:intergenic | 40.0% | |
| CAAGTTAATTCCCACCCATA+TGG | - | chr3.1:73176535-73176554 | None:intergenic | 40.0% | |
| CATAGTCGAATGACTCAAGT+TGG | + | chr3.1:73177258-73177277 | MS.gene06949:CDS | 40.0% | |
| CGAAGCAATGTCAAGCATAT+TGG | + | chr3.1:73177659-73177678 | MS.gene06949:CDS | 40.0% | |
| CGTATCATGTTATGAGCAAG+CGG | - | chr3.1:73177696-73177715 | None:intergenic | 40.0% | |
| CTTTCAAAGGATGTATGGCT+TGG | + | chr3.1:73177283-73177302 | MS.gene06949:CDS | 40.0% | |
| GTCAAGCATATTGGAATGGT+TGG | + | chr3.1:73177668-73177687 | MS.gene06949:CDS | 40.0% | |
| TCTCTTGAATCCCGAAAACA+TGG | - | chr3.1:73176840-73176859 | None:intergenic | 40.0% | |
| TGATTCATGTTCCTTCCAAG+GGG | - | chr3.1:73177144-73177163 | None:intergenic | 40.0% | |
| TGCAAGTCTGTATCAAGAGA+TGG | + | chr3.1:73176606-73176625 | MS.gene06949:CDS | 40.0% | |
| TGGACCTTTCAAAGGATGTA+TGG | + | chr3.1:73177278-73177297 | MS.gene06949:CDS | 40.0% | |
| ! | AATTCTGAGAAGGAGGTGAT+TGG | + | chr3.1:73176220-73176239 | MS.gene06949:CDS | 40.0% |
| ! | AATTTTTACTCAGGTCCCCT+TGG | + | chr3.1:73177126-73177145 | MS.gene06949:CDS | 40.0% |
| ! | GCATGATGCTAATTCTGAGA+AGG | + | chr3.1:73176210-73176229 | MS.gene06949:CDS | 40.0% |
| ! | TGATGCTAATTCTGAGAAGG+AGG | + | chr3.1:73176213-73176232 | MS.gene06949:CDS | 40.0% |
| ! | TGCCATGATCCGATTTTGAA+AGG | + | chr3.1:73176466-73176485 | MS.gene06949:CDS | 40.0% |
| ! | TTTCTTTGAAAGCCTAGCCA+CGG | - | chr3.1:73176446-73176465 | None:intergenic | 40.0% |
| !! | ACTCAAGTTGGACCTTTCAA+AGG | + | chr3.1:73177270-73177289 | MS.gene06949:CDS | 40.0% |
| !! | TGTTTTCGGGATTCAAGAGA+TGG | + | chr3.1:73176840-73176859 | MS.gene06949:CDS | 40.0% |
| AAACACTCTTCCGCCATAAC+TGG | + | chr3.1:73177216-73177235 | MS.gene06949:CDS | 45.0% | |
| AACACTCTTCCGCCATAACT+GGG | + | chr3.1:73177217-73177236 | MS.gene06949:CDS | 45.0% | |
| AAGGGGTCCTGAAGAAAATC+TGG | - | chr3.1:73176983-73177002 | None:intergenic | 45.0% | |
| ACACAAGTCTTGCAGGAGAA+TGG | + | chr3.1:73177634-73177653 | MS.gene06949:CDS | 45.0% | |
| ACTACGGGTACAGATCCATA+TGG | + | chr3.1:73176517-73176536 | MS.gene06949:CDS | 45.0% | |
| AGGACCTGAATGAGAAATGC+TGG | - | chr3.1:73177052-73177071 | None:intergenic | 45.0% | |
| AGTTTCCTTCCCAGTTATGG+CGG | - | chr3.1:73177229-73177248 | None:intergenic | 45.0% | |
| ATTCAGACCCACAAGAAGCT+CGG | - | chr3.1:73177829-73177848 | None:intergenic | 45.0% | |
| CGTTGAGAATATGGCACATG+TGG | + | chr3.1:73176414-73176433 | MS.gene06949:CDS | 45.0% | |
| CTACGGGTACAGATCCATAT+GGG | + | chr3.1:73176518-73176537 | MS.gene06949:CDS | 45.0% | |
| GAACGCCACTTGCATTTGAA+TGG | - | chr3.1:73177332-73177351 | None:intergenic | 45.0% | |
| GAAGCCATATACGAAGACCT+TGG | + | chr3.1:73177593-73177612 | MS.gene06949:CDS | 45.0% | |
| GAATTAGCATCATGCTTCCG+AGG | - | chr3.1:73176205-73176224 | None:intergenic | 45.0% | |
| GACTGCCATTCAAATGCAAG+TGG | + | chr3.1:73177324-73177343 | MS.gene06949:CDS | 45.0% | |
| GATGCCAAGGTCTTCGTATA+TGG | - | chr3.1:73177600-73177619 | None:intergenic | 45.0% | |
| GGAGCTCAATGCAAAATCTC+TGG | + | chr3.1:73177869-73177888 | MS.gene06949:CDS | 45.0% | |
| GGTGACTCATCCACAAAATC+AGG | + | chr3.1:73177075-73177094 | MS.gene06949:CDS | 45.0% | |
| GTACCAGCAGCACATTTGTT+CGG | - | chr3.1:73177761-73177780 | None:intergenic | 45.0% | |
| GTCTGGGTAACATGTTGTAC+AGG | - | chr3.1:73177545-73177564 | None:intergenic | 45.0% | |
| GTCTTTGACACAAGTCTTGC+AGG | + | chr3.1:73177627-73177646 | MS.gene06949:CDS | 45.0% | |
| GTGAGTTTCCTTCCCAGTTA+TGG | - | chr3.1:73177232-73177251 | None:intergenic | 45.0% | |
| TACCAGCAGCACATTTGTTC+GGG | - | chr3.1:73177760-73177779 | None:intergenic | 45.0% | |
| TGAATGGCAGTCAATGACAG+AGG | - | chr3.1:73177316-73177335 | None:intergenic | 45.0% | |
| TGATAAAGCAGCTGCACCAA+GGG | - | chr3.1:73177442-73177461 | None:intergenic | 45.0% | |
| TTTGTGGATGAGTCACCAAG+AGG | - | chr3.1:73177072-73177091 | None:intergenic | 45.0% | |
| ! | AAAGACTGATGACGATGCCA+AGG | - | chr3.1:73177613-73177632 | None:intergenic | 45.0% |
| ! | AGTTGCATGGAAAAGGCATG+AGG | + | chr3.1:73176717-73176736 | MS.gene06949:CDS | 45.0% |
| ! | CAAGAGATGGTAGATGATGG+TGG | + | chr3.1:73176853-73176872 | MS.gene06949:CDS | 45.0% |
| !! | ATGGCTTCAATTTGGCCTTG+AGG | - | chr3.1:73177581-73177600 | None:intergenic | 45.0% |
| !! | TGGCTTCAATTTGGCCTTGA+GGG | - | chr3.1:73177580-73177599 | None:intergenic | 45.0% |
| ACGTGTGAGAATTGCCCTCA+AGG | + | chr3.1:73177563-73177582 | MS.gene06949:CDS | 50.0% | |
| ACTTGCGGGATGTTTCTCTG+TGG | + | chr3.1:73176746-73176765 | MS.gene06949:CDS | 50.0% | |
| AGCAAGCTTGATGTCCAAGC+TGG | + | chr3.1:73176261-73176280 | MS.gene06949:CDS | 50.0% | |
| AGGGCAATTCTCACACGTCT+GGG | - | chr3.1:73177561-73177580 | None:intergenic | 50.0% | |
| CATGGTTTCGTAGTCTGTGG+AGG | + | chr3.1:73176176-73176195 | MS.gene06949:CDS | 50.0% | |
| GCAAAATCTCTGGCAGAGTG+TGG | + | chr3.1:73177879-73177898 | MS.gene06949:CDS | 50.0% | |
| GCAGCTTCTCCACACTTGAT+CGG | + | chr3.1:73177501-73177520 | MS.gene06949:CDS | 50.0% | |
| GCATGAGGTGAAGAACTTGC+GGG | + | chr3.1:73176732-73176751 | MS.gene06949:CDS | 50.0% | |
| GCGCCATCCGACTCATTATT+AGG | - | chr3.1:73176673-73176692 | None:intergenic | 50.0% | |
| GGCATGAGGTGAAGAACTTG+CGG | + | chr3.1:73176731-73176750 | MS.gene06949:CDS | 50.0% | |
| GGGATGTTTCTCTGTGGAAC+AGG | + | chr3.1:73176752-73176771 | MS.gene06949:CDS | 50.0% | |
| GGGTACAGATCCATATGGGT+GGG | + | chr3.1:73176522-73176541 | MS.gene06949:CDS | 50.0% | |
| GTGATAAAGCAGCTGCACCA+AGG | - | chr3.1:73177443-73177462 | None:intergenic | 50.0% | |
| TTCCCGAACAAATGTGCTGC+TGG | + | chr3.1:73177755-73177774 | MS.gene06949:CDS | 50.0% | |
| TTTACTCAGGTCCCCTTGGA+AGG | + | chr3.1:73177130-73177149 | MS.gene06949:CDS | 50.0% | |
| ! | GGCAGAGTGTGGCAGTTTTA+GGG | + | chr3.1:73177890-73177909 | MS.gene06949:CDS | 50.0% |
| ! | TGGCAGAGTGTGGCAGTTTT+AGG | + | chr3.1:73177889-73177908 | MS.gene06949:CDS | 50.0% |
| !! | GATGGAGGTGCTTGTTGATC+TGG | + | chr3.1:73176624-73176643 | MS.gene06949:CDS | 50.0% |
| !!! | GAGTGTGGCAGTTTTAGGGT+TGG | + | chr3.1:73177894-73177913 | MS.gene06949:CDS | 50.0% |
| AACCCTCCACCTGAAACCCT+TGG | + | chr3.1:73177423-73177442 | MS.gene06949:CDS | 55.0% | |
| CGGGTACAGATCCATATGGG+TGG | + | chr3.1:73176521-73176540 | MS.gene06949:CDS | 55.0% | |
| CTCTTCCGCCATAACTGGGA+AGG | + | chr3.1:73177221-73177240 | MS.gene06949:CDS | 55.0% | |
| GAGGGCAATTCTCACACGTC+TGG | - | chr3.1:73177562-73177581 | None:intergenic | 55.0% | |
| GTAGTCTGTGGAGGACTCCT+CGG | + | chr3.1:73176185-73176204 | MS.gene06949:CDS | 55.0% | |
| TGAAAGCCTAGCCACGGACT+CGG | - | chr3.1:73176440-73176459 | None:intergenic | 55.0% | |
| ! | CACCAAGGGTTTCAGGTGGA+GGG | - | chr3.1:73177428-73177447 | None:intergenic | 55.0% |
| GCAGCTGCACCAAGGGTTTC+AGG | - | chr3.1:73177435-73177454 | None:intergenic | 60.0% | |
| GCGTCGAGACCGATCAAGTG+TGG | - | chr3.1:73177513-73177532 | None:intergenic | 60.0% | |
| GCTGCACCAAGGGTTTCAGG+TGG | - | chr3.1:73177432-73177451 | None:intergenic | 60.0% | |
| ! | GCACCAAGGGTTTCAGGTGG+AGG | - | chr3.1:73177429-73177448 | None:intergenic | 60.0% |
| ATGTGGCCGAGTCCGTGGCT+AGG | + | chr3.1:73176431-73176450 | MS.gene06949:CDS | 65.0% | |
| GGCACATGTGGCCGAGTCCG+TGG | + | chr3.1:73176426-73176445 | MS.gene06949:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 73176163 | 73177944 | 73176163 | ID=MS.gene06949 |
| chr3.1 | mRNA | 73176163 | 73177944 | 73176163 | ID=MS.gene06949.t1;Parent=MS.gene06949 |
| chr3.1 | exon | 73176163 | 73177944 | 73176163 | ID=MS.gene06949.t1.exon1;Parent=MS.gene06949.t1 |
| chr3.1 | CDS | 73176163 | 73177944 | 73176163 | ID=cds.MS.gene06949.t1;Parent=MS.gene06949.t1 |
| Gene Sequence |
| Protein sequence |
