AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069590.t1 MTR_8g099495 96.581 234 8 0 1 234 175 408 2.57e-156 439
MS.gene069590.t1 MTR_8g099515 48.430 223 104 5 17 228 174 396 9.35e-64 204
MS.gene069590.t1 MTR_3g091200 35.714 238 135 6 3 223 145 381 5.86e-48 162
MS.gene069590.t1 MTR_5g009200 42.453 212 109 6 18 219 188 396 5.10e-45 155
MS.gene069590.t1 MTR_5g009210 42.920 226 116 6 3 219 140 361 1.28e-44 153
MS.gene069590.t1 MTR_5g009190 40.870 230 114 7 3 219 120 340 9.63e-43 147
MS.gene069590.t1 MTR_8g027745 35.749 207 126 3 22 221 156 362 1.70e-34 127
MS.gene069590.t1 MTR_3g012550 41.912 136 73 2 92 221 21 156 3.49e-32 115
MS.gene069590.t1 MTR_7g117350 32.618 233 137 5 5 227 146 368 1.94e-31 119
MS.gene069590.t1 MTR_7g117350 31.535 241 137 5 5 227 146 376 1.60e-30 116
MS.gene069590.t1 MTR_7g079280 34.194 155 87 3 92 231 15 169 5.71e-24 94.7
MS.gene069590.t1 MTR_8g028465 47.826 92 41 2 143 231 88 175 3.67e-22 90.5
MS.gene069590.t1 MTR_1g070140 37.008 127 74 2 94 215 47 172 1.49e-17 80.5
MS.gene069590.t1 MTR_6g007737 38.281 128 69 3 94 215 78 201 1.70e-17 80.9
MS.gene069590.t1 MTR_7g079290 41.558 77 43 1 143 217 123 199 1.90e-17 78.2
MS.gene069590.t1 MTR_1g090480 31.746 126 82 2 96 218 23 147 2.76e-17 76.6
MS.gene069590.t1 MTR_1g492830 29.787 141 86 4 96 230 13 146 1.73e-16 75.9
MS.gene069590.t1 MTR_5g034790 48.611 72 35 1 145 216 116 185 1.87e-16 77.8
MS.gene069590.t1 MTR_8g063660 47.887 71 35 1 145 215 68 136 3.88e-16 76.6
MS.gene069590.t1 MTR_4g106680 33.333 132 70 5 94 215 51 174 4.50e-16 76.3
MS.gene069590.t1 MTR_7g096690 38.710 93 55 1 134 224 59 151 6.89e-16 75.9
MS.gene069590.t1 MTR_1g056350 37.500 104 61 2 132 231 61 164 7.41e-16 73.2
MS.gene069590.t1 MTR_1g074190 45.679 81 40 2 139 215 93 173 4.08e-15 73.6
MS.gene069590.t1 MTR_8g066780 30.345 145 92 3 96 231 58 202 6.93e-15 73.2
MS.gene069590.t1 MTR_1g043410 37.255 102 56 3 117 215 69 165 7.53e-15 72.8
MS.gene069590.t1 MTR_7g116220 41.772 79 33 2 145 216 70 142 1.11e-14 70.1
MS.gene069590.t1 MTR_3g078470 37.179 78 49 0 139 216 70 147 1.13e-14 70.9
MS.gene069590.t1 MTR_3g078450 37.179 78 49 0 139 216 70 147 1.59e-14 70.5
MS.gene069590.t1 MTR_3g100400 42.254 71 39 1 145 215 130 198 2.14e-14 72.0
MS.gene069590.t1 MTR_7g076250 29.787 141 93 3 96 233 13 150 2.99e-14 69.3
MS.gene069590.t1 MTR_4g030070 44.444 72 37 1 144 215 84 152 3.47e-14 69.7
MS.gene069590.t1 MTR_4g063070 34.259 108 60 2 119 215 271 378 4.12e-14 71.2
MS.gene069590.t1 MTR_8g074900 39.726 73 38 1 145 217 158 224 4.63e-14 69.3
MS.gene069590.t1 MTR_5g092790 33.333 75 50 0 143 217 155 229 6.22e-14 69.3
MS.gene069590.t1 MTR_1g090460 48.276 58 27 1 164 218 83 140 7.10e-14 67.4
MS.gene069590.t1 MTR_3g110190 51.020 49 24 0 173 221 92 140 7.44e-14 70.1
MS.gene069590.t1 MTR_8g092840 38.462 78 47 1 139 216 107 183 7.84e-14 68.6
MS.gene069590.t1 MTR_3g094000 38.889 72 44 0 145 216 91 162 9.43e-14 67.4
MS.gene069590.t1 MTR_8g092960 40.278 72 42 1 145 216 103 173 1.17e-13 68.2
MS.gene069590.t1 MTR_8g092985 40.278 72 42 1 145 216 111 181 1.39e-13 67.8
MS.gene069590.t1 MTR_4g108830 31.579 95 62 1 122 216 98 189 1.47e-13 67.4
MS.gene069590.t1 MTR_6g087150 28.346 127 84 3 93 216 40 162 1.70e-13 69.3
MS.gene069590.t1 MTR_5g030010 31.111 135 76 5 96 215 58 190 1.84e-13 68.9
MS.gene069590.t1 MTR_8g092930 40.278 72 42 1 145 216 112 182 2.31e-13 67.4
MS.gene069590.t1 MTR_3g071300 33.333 114 72 2 122 232 53 165 2.33e-13 66.2
MS.gene069590.t1 MTR_8g032690 47.619 63 33 0 171 233 17 79 2.41e-13 64.7
MS.gene069590.t1 MTR_4g108830 31.250 96 63 1 121 216 125 217 2.60e-13 67.0
MS.gene069590.t1 MTR_5g092750 34.667 75 49 0 143 217 155 229 2.76e-13 67.4
MS.gene069590.t1 MTR_7g113800 41.096 73 40 2 145 215 91 162 4.18e-13 68.2
MS.gene069590.t1 MTR_8g093010 40.278 72 42 1 145 216 107 177 4.25e-13 66.6
MS.gene069590.t1 MTR_4g129420 34.711 121 65 5 100 215 75 186 4.59e-13 68.2
MS.gene069590.t1 MTR_8g089880 39.759 83 46 2 135 215 115 195 4.68e-13 67.4
MS.gene069590.t1 MTR_7g088560 30.252 119 74 3 103 215 63 178 6.81e-13 65.5
MS.gene069590.t1 MTR_7g116210 48.000 50 26 0 167 216 29 78 7.27e-13 63.5
MS.gene069590.t1 MTR_7g116180 40.506 79 34 2 145 216 67 139 8.82e-13 65.1
MS.gene069590.t1 MTR_1g070425 41.096 73 42 1 145 216 68 140 1.18e-12 64.7
MS.gene069590.t1 MTR_2g096350 38.028 71 44 0 146 216 108 178 1.24e-12 65.1
MS.gene069590.t1 MTR_5g022920 36.620 71 45 0 145 215 161 231 1.31e-12 65.5
MS.gene069590.t1 MTR_3g091620 38.158 76 37 2 145 216 101 170 1.34e-12 66.6
MS.gene069590.t1 MTR_5g064990 43.056 72 40 1 145 215 114 185 1.35e-12 66.6
MS.gene069590.t1 MTR_7g116200 48.000 50 26 0 167 216 87 136 1.39e-12 64.3
MS.gene069590.t1 MTR_8g023400 46.774 62 29 1 173 234 117 174 1.78e-12 65.9
MS.gene069590.t1 MTR_4g120270 51.163 43 21 0 173 215 104 146 1.93e-12 65.5
MS.gene069590.t1 MTR_4g093830 37.349 83 48 2 135 215 114 194 2.41e-12 65.9
MS.gene069590.t1 MTR_7g073200 41.096 73 39 2 145 216 81 150 2.64e-12 63.5
MS.gene069590.t1 MTR_5g064980 40.278 72 42 1 145 215 108 179 2.68e-12 65.9
MS.gene069590.t1 MTR_3g095560 36.620 71 44 1 145 215 109 178 2.82e-12 64.7
MS.gene069590.t1 MTR_3g099650 50.000 58 26 2 173 227 136 193 3.04e-12 65.5
MS.gene069590.t1 MTR_4g056590 40.845 71 37 1 145 215 100 165 3.84e-12 63.5
MS.gene069590.t1 MTR_8g092990 51.724 58 27 1 159 215 126 183 4.04e-12 63.9
MS.gene069590.t1 MTR_5g026730 46.154 52 28 0 165 216 86 137 4.28e-12 62.8
MS.gene069590.t1 MTR_8g092970 51.724 58 27 1 159 215 116 173 4.42e-12 63.5
MS.gene069590.t1 MTR_3g081030 37.500 72 42 1 144 215 93 161 4.96e-12 63.5
MS.gene069590.t1 MTR_3g116000 32.174 115 69 3 103 217 43 148 5.08e-12 63.2
MS.gene069590.t1 MTR_1g011380 48.214 56 28 1 161 216 329 383 5.32e-12 65.1
MS.gene069590.t1 MTR_2g044570 52.273 44 21 0 173 216 83 126 5.38e-12 64.3
MS.gene069590.t1 MTR_5g064890 33.333 96 57 2 120 215 108 196 5.41e-12 64.7
MS.gene069590.t1 MTR_2g090905 43.750 64 31 2 154 215 53 113 5.87e-12 61.6
MS.gene069590.t1 MTR_1g032440 55.769 52 22 1 164 215 290 340 7.64e-12 64.3
MS.gene069590.t1 MTR_7g117540 53.488 43 20 0 173 215 101 143 8.05e-12 63.9
MS.gene069590.t1 MTR_2g079320 44.828 58 32 0 159 216 145 202 1.05e-11 62.4
MS.gene069590.t1 MTR_2g009310 30.392 102 66 3 116 215 105 203 1.08e-11 63.9
MS.gene069590.t1 MTR_1g082550 53.488 43 20 0 173 215 127 169 1.37e-11 63.5
MS.gene069590.t1 MTR_5g026720 52.273 44 21 0 173 216 93 136 1.75e-11 60.8
MS.gene069590.t1 MTR_5g066250 38.889 72 40 2 145 215 134 202 3.09e-11 62.8
MS.gene069590.t1 MTR_6g055030 40.000 60 35 1 173 231 126 185 5.56e-11 61.6
MS.gene069590.t1 MTR_2g055790 26.174 149 99 3 68 216 6 143 5.59e-11 60.5
MS.gene069590.t1 MTR_4g094725 33.333 75 50 0 145 219 78 152 5.95e-11 59.3
MS.gene069590.t1 MTR_5g024730 34.247 73 48 0 145 217 77 149 6.80e-11 60.5
MS.gene069590.t1 MTR_5g082940 30.682 88 61 0 145 232 75 162 7.24e-11 59.3
MS.gene069590.t1 MTR_3g107995 44.643 56 30 1 161 216 305 359 7.97e-11 61.6
MS.gene069590.t1 MTR_8g009190 48.837 43 22 0 173 215 105 147 8.61e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069590.t1 AT5G53110 38.914 221 121 3 14 220 157 377 1.48e-48 164
MS.gene069590.t1 AT5G07040 40.909 154 82 4 82 231 7 155 1.38e-34 121
MS.gene069590.t1 AT2G25410 37.853 177 103 5 49 218 196 372 8.58e-34 125
MS.gene069590.t1 AT2G46495 35.122 205 122 5 22 218 164 365 3.36e-33 123
MS.gene069590.t1 AT1G28040 33.333 207 123 5 22 216 93 296 3.09e-31 117
MS.gene069590.t1 AT2G46494 33.000 200 126 4 22 216 163 359 2.38e-29 113
MS.gene069590.t1 AT2G46495 33.990 203 99 6 22 218 164 337 3.59e-29 112
MS.gene069590.t1 AT2G46160 47.059 102 41 2 143 231 102 203 1.33e-23 94.7
MS.gene069590.t1 AT2G46160 47.059 102 41 2 143 231 102 203 1.33e-23 94.7
MS.gene069590.t1 AT2G46493 56.944 72 31 0 145 216 110 181 4.03e-23 92.8
MS.gene069590.t1 AT3G61550 42.991 107 45 2 141 231 95 201 4.03e-22 90.9
MS.gene069590.t1 AT2G35910 36.000 150 81 5 91 231 65 208 1.71e-18 81.3
MS.gene069590.t1 AT2G35000 31.624 117 76 2 103 215 61 177 8.20e-17 79.0
MS.gene069590.t1 AT5G06490 29.762 168 95 4 90 234 23 190 8.31e-17 76.3
MS.gene069590.t1 AT5G36001 28.000 200 102 6 20 219 153 310 2.89e-16 76.6
MS.gene069590.t1 AT1G23980 35.659 129 74 4 94 215 60 186 5.51e-16 76.3
MS.gene069590.t1 AT5G05810 45.833 72 35 2 145 215 120 188 1.10e-15 75.9
MS.gene069590.t1 AT1G04360 35.345 116 70 1 124 234 80 195 1.44e-15 75.5
MS.gene069590.t1 AT3G03550 64.444 45 16 0 173 217 159 203 4.06e-15 73.9
MS.gene069590.t1 AT5G66070 34.615 130 68 4 99 216 95 219 5.46e-15 72.0
MS.gene069590.t1 AT5G66070 34.615 130 68 4 99 216 95 219 5.46e-15 72.0
MS.gene069590.t1 AT2G20030 44.444 72 38 1 145 216 98 167 6.88e-15 73.6
MS.gene069590.t1 AT3G05200 40.860 93 41 3 145 229 99 185 8.32e-15 73.2
MS.gene069590.t1 AT5G43420 57.692 52 22 0 173 224 138 189 1.73e-14 72.0
MS.gene069590.t1 AT4G28890 43.056 72 39 1 145 216 97 166 1.87e-14 72.4
MS.gene069590.t1 AT5G66070 40.278 72 39 1 145 216 176 243 2.80e-14 70.5
MS.gene069590.t1 AT1G33480 45.833 72 36 1 144 215 74 142 3.12e-14 70.5
MS.gene069590.t1 AT5G17600 60.000 45 18 0 173 217 142 186 3.60e-14 71.2
MS.gene069590.t1 AT2G27940 34.694 98 60 3 120 216 89 183 4.38e-14 69.7
MS.gene069590.t1 AT1G72200 45.833 72 38 1 145 215 115 186 4.54e-14 71.2
MS.gene069590.t1 AT5G40250 43.210 81 34 2 145 215 107 185 7.67e-14 70.5
MS.gene069590.t1 AT4G40070 42.466 73 41 1 145 216 95 167 7.99e-14 70.1
MS.gene069590.t1 AT4G17905 45.070 71 39 0 145 215 153 223 9.06e-14 69.7
MS.gene069590.t1 AT4G17905 45.070 71 39 0 145 215 127 197 1.07e-13 69.3
MS.gene069590.t1 AT4G09130 43.056 72 40 1 145 215 91 162 1.10e-13 69.7
MS.gene069590.t1 AT5G57750 42.667 75 34 2 146 216 96 165 1.27e-13 67.8
MS.gene069590.t1 AT3G48030 44.048 84 40 3 145 227 102 179 1.46e-13 68.6
MS.gene069590.t1 AT3G48030 44.048 84 40 3 145 227 180 257 1.85e-13 68.9
MS.gene069590.t1 AT2G34990 40.541 74 43 1 143 215 65 138 2.33e-13 68.6
MS.gene069590.t1 AT2G34990 40.541 74 43 1 143 215 87 160 2.43e-13 68.6
MS.gene069590.t1 AT1G80400 50.000 66 32 1 161 226 343 407 2.70e-13 68.9
MS.gene069590.t1 AT5G27420 42.353 85 35 3 145 221 95 173 3.44e-13 68.6
MS.gene069590.t1 AT4G09100 40.278 72 42 1 145 215 56 127 5.39e-13 64.3
MS.gene069590.t1 AT4G09120 43.056 72 40 1 145 215 94 165 7.94e-13 67.0
MS.gene069590.t1 AT1G22500 40.909 88 49 2 145 229 89 176 8.13e-13 67.4
MS.gene069590.t1 AT4G10150 31.343 134 84 3 87 215 23 153 9.28e-13 65.9
MS.gene069590.t1 AT2G17730 37.500 72 44 1 145 215 167 238 9.67e-13 65.9
MS.gene069590.t1 AT1G49220 41.667 72 41 1 145 216 108 178 1.03e-12 65.9
MS.gene069590.t1 AT5G01880 38.889 72 42 2 145 216 79 148 1.03e-12 64.3
MS.gene069590.t1 AT4G30400 53.488 43 20 0 173 215 134 176 1.15e-12 67.0
MS.gene069590.t1 AT4G10160 33.051 118 71 3 100 215 28 139 1.17e-12 65.5
MS.gene069590.t1 AT2G47560 38.028 71 44 0 145 215 80 150 1.23e-12 65.5
MS.gene069590.t1 AT4G35840 36.111 72 45 1 145 215 162 233 1.34e-12 65.5
MS.gene069590.t1 AT4G33565 38.554 83 47 2 135 215 179 259 1.47e-12 66.6
MS.gene069590.t1 AT2G18650 54.545 44 20 0 173 216 126 169 1.73e-12 66.6
MS.gene069590.t1 AT3G16720 48.936 47 24 0 173 219 119 165 2.04e-12 65.9
MS.gene069590.t1 AT1G72220 31.200 125 72 3 103 215 97 219 2.25e-12 66.2
MS.gene069590.t1 AT4G15975 51.064 47 23 0 173 219 76 122 2.60e-12 64.7
MS.gene069590.t1 AT3G20395 36.709 79 46 1 145 223 147 221 3.23e-12 64.3
MS.gene069590.t1 AT1G76410 32.520 123 62 6 100 217 42 148 3.63e-12 63.5
MS.gene069590.t1 AT1G72310 50.000 54 24 1 173 223 127 180 3.78e-12 65.1
MS.gene069590.t1 AT1G49210 38.889 72 43 1 145 216 108 178 4.21e-12 63.9
MS.gene069590.t1 AT4G09110 41.667 72 41 1 145 215 94 165 4.32e-12 64.7
MS.gene069590.t1 AT5G10380 55.814 43 19 0 173 215 135 177 5.63e-12 64.3
MS.gene069590.t1 AT4G32600 55.319 47 20 1 170 216 360 405 6.36e-12 64.7
MS.gene069590.t1 AT4G32600 55.319 47 20 1 170 216 360 405 6.36e-12 64.7
MS.gene069590.t1 AT1G20823 31.405 121 68 5 103 217 44 155 9.44e-12 62.4
MS.gene069590.t1 AT4G11680 56.250 48 20 1 168 215 333 379 1.62e-11 63.5
MS.gene069590.t1 AT3G61180 48.214 56 28 1 160 215 310 364 1.64e-11 63.5
MS.gene069590.t1 AT2G35420 30.952 126 81 3 90 215 26 145 1.76e-11 62.8
MS.gene069590.t1 AT1G49200 36.111 72 45 1 145 216 109 179 2.97e-11 61.6
MS.gene069590.t1 AT3G14320 50.000 44 22 0 173 216 88 131 3.27e-11 61.2
MS.gene069590.t1 AT2G42350 32.292 96 60 2 124 219 56 146 3.66e-11 61.2
MS.gene069590.t1 AT3G62690 48.837 43 22 0 173 215 113 155 4.02e-11 61.6
MS.gene069590.t1 AT3G18773 37.500 72 44 1 145 216 103 173 4.67e-11 60.8
MS.gene069590.t1 AT1G35330 53.488 43 20 0 173 215 129 171 4.79e-11 62.0
MS.gene069590.t1 AT3G60220 52.273 44 21 0 173 216 117 160 4.95e-11 62.0
MS.gene069590.t1 AT1G53820 50.000 44 22 0 173 216 120 163 5.34e-11 61.6
MS.gene069590.t1 AT2G17450 34.615 78 46 2 145 217 69 146 5.64e-11 60.1
MS.gene069590.t1 AT3G10910 39.437 71 41 2 145 215 88 156 5.91e-11 60.1
MS.gene069590.t1 AT1G53010 39.506 81 43 3 145 219 97 177 7.46e-11 59.7

Find 53 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGTCCTCAATACTTCATT+TGG 0.314741 4.4:+4825712 None:intergenic
AATGATAATTGTTAAGAAGT+TGG 0.336693 4.4:+4825562 None:intergenic
AAGAGACGAATTGGTTGTTT+TGG 0.346013 4.4:-4827023 MS.gene069590:CDS
TTGAAAATTCAGTGCTTGTT+TGG 0.351667 4.4:+4825881 None:intergenic
TTTCATGTGAATTGTATAGA+TGG 0.358575 4.4:-4825670 MS.gene069590:CDS
GAGGAAGATGTGGATTGGTT+GGG 0.360207 4.4:-4826933 MS.gene069590:CDS
TGTTAAGAAGTTGGAGATAC+AGG 0.363370 4.4:+4825571 None:intergenic
ATCTTCCACTTTCACCAAGC+TGG 0.369581 4.4:+4825785 None:intergenic
CTTTCAAGGAAAGTCAAGTA+TGG 0.374180 4.4:-4826000 MS.gene069590:CDS
GTATGGTTTAAGCCTAGGTT+TGG 0.409546 4.4:-4825983 MS.gene069590:CDS
TATGGTTTAAGCCTAGGTTT+GGG 0.424366 4.4:-4825982 MS.gene069590:CDS
GCTTCACAATTTCCACAATC+AGG 0.434686 4.4:+4826961 None:intergenic
TCTTCCACTTTCACCAAGCT+GGG 0.464098 4.4:+4825786 None:intergenic
AGCCTAGGTTTGGGAATACC+TGG 0.477958 4.4:-4825973 MS.gene069590:CDS
GGAGGAAGATGTGGATTGGT+TGG 0.478263 4.4:-4826934 MS.gene069590:CDS
TACCGGTGGAAGAGACGAAT+TGG 0.478685 4.4:-4827032 MS.gene069590:CDS
GTCAAGTATGGTTTAAGCCT+AGG 0.480880 4.4:-4825988 MS.gene069590:CDS
GTCTGCATGTAGGTCTTTCA+AGG 0.481860 4.4:-4826014 MS.gene069590:intron
CTGGAAAATTTCGACACAAA+GGG 0.482093 4.4:+4825623 None:intergenic
TAGGTCTAATTGTGTTTACA+TGG 0.490881 4.4:-4825945 MS.gene069590:CDS
AGGAAGATGTGGATTGGTTG+GGG 0.491695 4.4:-4826932 MS.gene069590:CDS
AAAACCCAGCTTGGTGAAAG+TGG 0.493961 4.4:-4825790 MS.gene069590:CDS
TTGGGAATACCTGGACTCCT+AGG 0.494340 4.4:-4825964 MS.gene069590:CDS
ATTGACTATTTCACCTTGGG+TGG 0.497251 4.4:+4826876 None:intergenic
GAAAACGAAGTAGATCTCTC+TGG 0.499315 4.4:+4825604 None:intergenic
CAAATGTTGTCACTTGGCCT+AGG 0.507133 4.4:+4825760 None:intergenic
GATGTCCACGGTGTAGCAAA+AGG 0.511204 4.4:+4827084 None:intergenic
GAACATGAATTATTCGATTG+TGG 0.526227 4.4:-4827124 None:intergenic
TATTTCGTCAGCTTTGATAC+CGG 0.536087 4.4:-4827049 MS.gene069590:CDS
AACCAATTCGTCTCTTCCAC+CGG 0.549752 4.4:+4827030 None:intergenic
ACAATTAGACCTAGGAGTCC+AGG 0.561924 4.4:+4825955 None:intergenic
TTCTAACATACCGTTGCAGT+GGG 0.563982 4.4:-4826989 MS.gene069590:CDS
TGTTATGATCTTCCCACCCA+AGG 0.578358 4.4:-4826889 MS.gene069590:intron
ATCAGCCAAATGAAGTATTG+AGG 0.579109 4.4:-4825717 MS.gene069590:CDS
AGATACCCTAAAACCCAGCT+TGG 0.586664 4.4:-4825799 MS.gene069590:CDS
ATGTGAATTGTATAGATGGA+TGG 0.587383 4.4:-4825666 MS.gene069590:CDS
ATTCTAACATACCGTTGCAG+TGG 0.592135 4.4:-4826990 MS.gene069590:CDS
TCTGGAAAATTTCGACACAA+AGG 0.592383 4.4:+4825622 None:intergenic
CAGTGGGATGATCCTGATTG+TGG 0.594321 4.4:-4826973 MS.gene069590:CDS
TTCGTCAGCTTTGATACCGG+TGG 0.597171 4.4:-4827046 MS.gene069590:CDS
TTGACTATTTCACCTTGGGT+GGG 0.599087 4.4:+4826877 None:intergenic
GTGAAAGTGGAAGATTGCCT+AGG 0.611829 4.4:-4825777 MS.gene069590:CDS
ATAGAGCAAATGTTGTCACT+TGG 0.619697 4.4:+4825754 None:intergenic
TGAAAATAGTGATTACACTC+AGG 0.636381 4.4:+4825688 None:intergenic
AATTCACATAATGATGTCCA+CGG 0.636628 4.4:+4827072 None:intergenic
TCAGGATCATCCCACTGCAA+CGG 0.649351 4.4:+4826979 None:intergenic
GAAGCACGTGGAGGAAGATG+TGG 0.650882 4.4:-4826943 MS.gene069590:CDS
ATGTAAACACAATTAGACCT+AGG 0.654229 4.4:+4825947 None:intergenic
ACGTGGAGGAAGATGTGGAT+TGG 0.656368 4.4:-4826938 MS.gene069590:CDS
GTCCACGGTGTAGCAAAAGG+AGG 0.695275 4.4:+4827087 None:intergenic
GTCCAGGTATTCCCAAACCT+AGG 0.704709 4.4:+4825971 None:intergenic
TGTGGAAATTGTGAAGCACG+TGG 0.742242 4.4:-4826955 MS.gene069590:CDS
GGAAATTGTGAAGCACGTGG+AGG 0.769055 4.4:-4826952 MS.gene069590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATATCAAAATAGATA+CGG - chr4.4:4825968-4825987 MS.gene069590:CDS 10.0%
!! AACAAGTTATGTAATTAATT+AGG + chr4.4:4826627-4826646 None:intergenic 15.0%
!!! TTTTGATATTTTTTTTTCCA+AGG + chr4.4:4825962-4825981 None:intergenic 15.0%
!! AAAAAACATGGAGTTTAATT+TGG - chr4.4:4826074-4826093 MS.gene069590:intron 20.0%
!! AAAATCAAATGAAACAAATG+CGG - chr4.4:4826117-4826136 MS.gene069590:intron 20.0%
!!! AGTTTCAATTTAGATTAGTT+CGG - chr4.4:4826287-4826306 MS.gene069590:intron 20.0%
!!! TGCAAATTTAGATCATATTT+TGG - chr4.4:4825864-4825883 MS.gene069590:CDS 20.0%
!!! TTTGGTTTTCTTTTAACTTT+TGG - chr4.4:4825882-4825901 MS.gene069590:CDS 20.0%
!!! TTTTTTTTCCAAGGATTATT+TGG + chr4.4:4825953-4825972 None:intergenic 20.0%
! ACATTTGATTCAGATGTATT+TGG + chr4.4:4826258-4826277 None:intergenic 25.0%
! AGAAATATCCAAATAATCCT+TGG - chr4.4:4825942-4825961 MS.gene069590:CDS 25.0%
! TGATCTAAATTTGCAAGAAA+TGG + chr4.4:4825859-4825878 None:intergenic 25.0%
! TGTAATTAATTAGGATCCTT+AGG + chr4.4:4826618-4826637 None:intergenic 25.0%
! TTTCATGTGAATTGTATAGA+TGG - chr4.4:4827023-4827042 MS.gene069590:CDS 25.0%
!!! CAATTTTTTTGTGTTTAGCT+CGG - chr4.4:4826171-4826190 MS.gene069590:intron 25.0%
!!! TCCATGTTTTTTTTATCTTG+CGG + chr4.4:4826066-4826085 None:intergenic 25.0%
!!! TTTGGTTTTGTTTGATTTTG+AGG - chr4.4:4826023-4826042 MS.gene069590:intron 25.0%
AACTAAACGATTGTGTTTCA+TGG + chr4.4:4826402-4826421 None:intergenic 30.0%
AAGACCCATAACAAAAATTG+GGG + chr4.4:4826856-4826875 None:intergenic 30.0%
AATTCACATAATGATGTCCA+CGG + chr4.4:4825624-4825643 None:intergenic 30.0%
ACCGCAAGATAAAAAAAACA+TGG - chr4.4:4826062-4826081 MS.gene069590:intron 30.0%
ATGTAAACACAATTAGACCT+AGG + chr4.4:4826749-4826768 None:intergenic 30.0%
ATGTGAATTGTATAGATGGA+TGG - chr4.4:4827027-4827046 MS.gene069590:CDS 30.0%
ATTGTCCTCAATACTTCATT+TGG + chr4.4:4826984-4827003 None:intergenic 30.0%
CAAGACCCATAACAAAAATT+GGG + chr4.4:4826857-4826876 None:intergenic 30.0%
TAGGTCTAATTGTGTTTACA+TGG - chr4.4:4826748-4826767 MS.gene069590:intron 30.0%
TATGCAAAAAGAAGAACACA+AGG - chr4.4:4826775-4826794 MS.gene069590:intron 30.0%
TCAAGACCCATAACAAAAAT+TGG + chr4.4:4826858-4826877 None:intergenic 30.0%
TGAAAATAGTGATTACACTC+AGG + chr4.4:4827008-4827027 None:intergenic 30.0%
TGAAACACAATCGTTTAGTT+TGG - chr4.4:4826402-4826421 MS.gene069590:intron 30.0%
TTGAAAATTCAGTGCTTGTT+TGG + chr4.4:4826815-4826834 None:intergenic 30.0%
! ATAGTTGTGTTGTTCTCATT+TGG + chr4.4:4826354-4826373 None:intergenic 30.0%
! ATCATTGACTATTTCACCTT+GGG + chr4.4:4825823-4825842 None:intergenic 30.0%
! GAAACACAATCGTTTAGTTT+GGG - chr4.4:4826403-4826422 MS.gene069590:intron 30.0%
! TAGTTGTGTTGTTCTCATTT+GGG + chr4.4:4826353-4826372 None:intergenic 30.0%
! TTCTTTTCCGTTGTTCTTTT+GGG - chr4.4:4826656-4826675 MS.gene069590:intron 30.0%
! TTTCTTTTCCGTTGTTCTTT+TGG - chr4.4:4826655-4826674 MS.gene069590:intron 30.0%
!! ACATGGAGTTTAATTTGGTT+CGG - chr4.4:4826079-4826098 MS.gene069590:intron 30.0%
!!! ATTCATAGTGCATTTTGGTT+TGG - chr4.4:4826005-4826024 MS.gene069590:CDS 30.0%
!!! GTTTTTCTATTGTGTTGGTT+TGG - chr4.4:4826316-4826335 MS.gene069590:intron 30.0%
AAAATCCGCTTTAGTTGCAA+AGG + chr4.4:4826433-4826452 None:intergenic 35.0%
AATCTCCACTATAAGTCCTA+AGG - chr4.4:4826599-4826618 MS.gene069590:intron 35.0%
AGACCCATAACAAAAATTGG+GGG + chr4.4:4826855-4826874 None:intergenic 35.0%
ATAGAGCAAATGTTGTCACT+TGG + chr4.4:4826942-4826961 None:intergenic 35.0%
CAAATGAAACAAATGCGGTT+TGG - chr4.4:4826122-4826141 MS.gene069590:intron 35.0%
CTGGAAAATTTCGACACAAA+GGG + chr4.4:4827073-4827092 None:intergenic 35.0%
CTTTCAAGGAAAGTCAAGTA+TGG - chr4.4:4826693-4826712 MS.gene069590:intron 35.0%
TATGGTTTAAGCCTAGGTTT+GGG - chr4.4:4826711-4826730 MS.gene069590:intron 35.0%
TCTGGAAAATTTCGACACAA+AGG + chr4.4:4827074-4827093 None:intergenic 35.0%
! AAGAGACGAATTGGTTGTTT+TGG - chr4.4:4825670-4825689 MS.gene069590:CDS 35.0%
! AGTTGTGTTGTTCTCATTTG+GGG + chr4.4:4826352-4826371 None:intergenic 35.0%
! ATTTAGATTAGTTCGGTTCG+CGG - chr4.4:4826294-4826313 MS.gene069590:intron 35.0%
! CATCATTGACTATTTCACCT+TGG + chr4.4:4825824-4825843 None:intergenic 35.0%
! GAGATCTACTTCGTTTTCTT+CGG - chr4.4:4827094-4827113 MS.gene069590:CDS 35.0%
! TATTTCGTCAGCTTTGATAC+CGG - chr4.4:4825644-4825663 MS.gene069590:CDS 35.0%
! TCTTTTCCGTTGTTCTTTTG+GGG - chr4.4:4826657-4826676 MS.gene069590:intron 35.0%
!! ATCAGCCAAATGAAGTATTG+AGG - chr4.4:4826976-4826995 MS.gene069590:CDS 35.0%
!! GAGGTATTCATAGTGCATTT+TGG - chr4.4:4826000-4826019 MS.gene069590:CDS 35.0%
!! TTTTGTTATGGGTCTTGACA+GGG - chr4.4:4826860-4826879 MS.gene069590:intron 35.0%
!! TTTTTGTTATGGGTCTTGAC+AGG - chr4.4:4826859-4826878 MS.gene069590:intron 35.0%
AGGATCCTTAGGACTTATAG+TGG + chr4.4:4826607-4826626 None:intergenic 40.0%
ATTCTAACATACCGTTGCAG+TGG - chr4.4:4825703-4825722 MS.gene069590:CDS 40.0%
ATTGACTATTTCACCTTGGG+TGG + chr4.4:4825820-4825839 None:intergenic 40.0%
GAAAACGAAGTAGATCTCTC+TGG + chr4.4:4827092-4827111 None:intergenic 40.0%
GACCCATAACAAAAATTGGG+GGG + chr4.4:4826854-4826873 None:intergenic 40.0%
GCAAATTGAACTGAACCATG+CGG - chr4.4:4826212-4826231 MS.gene069590:intron 40.0%
GCATGGTTCAGTTCAATTTG+CGG + chr4.4:4826213-4826232 None:intergenic 40.0%
GCTTCACAATTTCCACAATC+AGG + chr4.4:4825735-4825754 None:intergenic 40.0%
GTATGGTTTAAGCCTAGGTT+TGG - chr4.4:4826710-4826729 MS.gene069590:intron 40.0%
GTCAAGTATGGTTTAAGCCT+AGG - chr4.4:4826705-4826724 MS.gene069590:intron 40.0%
GTCTACCTTTGCAACTAAAG+CGG - chr4.4:4826425-4826444 MS.gene069590:intron 40.0%
TTCTAACATACCGTTGCAGT+GGG - chr4.4:4825704-4825723 MS.gene069590:CDS 40.0%
TTGACTATTTCACCTTGGGT+GGG + chr4.4:4825819-4825838 None:intergenic 40.0%
! GTTGTGTTGTTCTCATTTGG+GGG + chr4.4:4826351-4826370 None:intergenic 40.0%
! TCGCGGTTTTTCTATTGTGT+TGG - chr4.4:4826311-4826330 MS.gene069590:intron 40.0%
! TTTCACCAAGCTGGGTTTTA+GGG + chr4.4:4826902-4826921 None:intergenic 40.0%
!! CACCCCCCAATTTTTGTTAT+GGG - chr4.4:4826849-4826868 MS.gene069590:intron 40.0%
!! GAAACAAATGCGGTTTGGTT+TGG - chr4.4:4826127-4826146 MS.gene069590:intron 40.0%
!!! CTTTTTTTTACTCAGCCGCA+TGG + chr4.4:4826230-4826249 None:intergenic 40.0%
AACCAATTCGTCTCTTCCAC+CGG + chr4.4:4825666-4825685 None:intergenic 45.0%
ACAATTAGACCTAGGAGTCC+AGG + chr4.4:4826741-4826760 None:intergenic 45.0%
AGATACCCTAAAACCCAGCT+TGG - chr4.4:4826894-4826913 MS.gene069590:CDS 45.0%
ATAGATACGGCGAGAACTAG+AGG - chr4.4:4825981-4826000 MS.gene069590:CDS 45.0%
ATCTTCCACTTTCACCAAGC+TGG + chr4.4:4826911-4826930 None:intergenic 45.0%
GTCTGCATGTAGGTCTTTCA+AGG - chr4.4:4826679-4826698 MS.gene069590:intron 45.0%
GTGAAAGTGGAAGATTGCCT+AGG - chr4.4:4826916-4826935 MS.gene069590:CDS 45.0%
TCTTCCACTTTCACCAAGCT+GGG + chr4.4:4826910-4826929 None:intergenic 45.0%
TGCAGACCCCAAAAGAACAA+CGG + chr4.4:4826666-4826685 None:intergenic 45.0%
TGTGGAAATTGTGAAGCACG+TGG - chr4.4:4825738-4825757 MS.gene069590:CDS 45.0%
TGTTATGATCTTCCCACCCA+AGG - chr4.4:4825804-4825823 MS.gene069590:CDS 45.0%
! AAAACCCAGCTTGGTGAAAG+TGG - chr4.4:4826903-4826922 MS.gene069590:CDS 45.0%
! CAAATGTTGTCACTTGGCCT+AGG + chr4.4:4826936-4826955 None:intergenic 45.0%
! CTTTCACCAAGCTGGGTTTT+AGG + chr4.4:4826903-4826922 None:intergenic 45.0%
! GCACCCCCCAATTTTTGTTA+TGG - chr4.4:4826848-4826867 MS.gene069590:intron 45.0%
!! AGGAAGATGTGGATTGGTTG+GGG - chr4.4:4825761-4825780 MS.gene069590:CDS 45.0%
!! GAGGAAGATGTGGATTGGTT+GGG - chr4.4:4825760-4825779 MS.gene069590:CDS 45.0%
!! GCGGTTTGGTTTGGTTGTTA+CGG - chr4.4:4826136-4826155 MS.gene069590:intron 45.0%
!!! TTCTTTTGGGGTCTGCATGT+AGG - chr4.4:4826669-4826688 MS.gene069590:intron 45.0%
ACGTGGAGGAAGATGTGGAT+TGG - chr4.4:4825755-4825774 MS.gene069590:CDS 50.0%
AGCCTAGGTTTGGGAATACC+TGG - chr4.4:4826720-4826739 MS.gene069590:intron 50.0%
CAGTGGGATGATCCTGATTG+TGG - chr4.4:4825720-4825739 MS.gene069590:CDS 50.0%
GATGTCCACGGTGTAGCAAA+AGG + chr4.4:4825612-4825631 None:intergenic 50.0%
GGAAATTGTGAAGCACGTGG+AGG - chr4.4:4825741-4825760 MS.gene069590:CDS 50.0%
GTCCAGGTATTCCCAAACCT+AGG + chr4.4:4826725-4826744 None:intergenic 50.0%
TACCGGTGGAAGAGACGAAT+TGG - chr4.4:4825661-4825680 MS.gene069590:CDS 50.0%
TCAGGATCATCCCACTGCAA+CGG + chr4.4:4825717-4825736 None:intergenic 50.0%
TTGGGAATACCTGGACTCCT+AGG - chr4.4:4826729-4826748 MS.gene069590:intron 50.0%
! ATCCTCCTTTTGCTACACCG+TGG - chr4.4:4825604-4825623 MS.gene069590:CDS 50.0%
! TGGGGGGTGCGTCATAATAA+AGG + chr4.4:4826838-4826857 None:intergenic 50.0%
! TTCGTCAGCTTTGATACCGG+TGG - chr4.4:4825647-4825666 MS.gene069590:CDS 50.0%
!! GGAGGAAGATGTGGATTGGT+TGG - chr4.4:4825759-4825778 MS.gene069590:CDS 50.0%
GAAGCACGTGGAGGAAGATG+TGG - chr4.4:4825750-4825769 MS.gene069590:CDS 55.0%
GTCCACGGTGTAGCAAAAGG+AGG + chr4.4:4825609-4825628 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 4825573 4827142 4825573 ID=MS.gene069590
chr4.4 mRNA 4825573 4827142 4825573 ID=MS.gene069590.t1;Parent=MS.gene069590
chr4.4 exon 4826890 4827142 4826890 ID=MS.gene069590.t1.exon1;Parent=MS.gene069590.t1
chr4.4 CDS 4826890 4827142 4826890 ID=cds.MS.gene069590.t1;Parent=MS.gene069590.t1
chr4.4 exon 4825573 4826024 4825573 ID=MS.gene069590.t1.exon2;Parent=MS.gene069590.t1
chr4.4 CDS 4825573 4826024 4825573 ID=cds.MS.gene069590.t1;Parent=MS.gene069590.t1
Gene Sequence

>MS.gene069590

ATGAATTATTCGATTGTGGCTATGATGTCTGATCCTCCTTTTGCTACACCGTGGACATCATTATGTGAATTTATTTCGTCAGCTTTGATACCGGTGGAAGAGACGAATTGGTTGTTTTGGACTGATTATTATTCTAACATACCGTTGCAGTGGGATGATCCTGATTGTGGAAATTGTGAAGCACGTGGAGGAAGATGTGGATTGGTTGGGGATGATGCTCTTCGTCTTGCTTGTTATGATCTTCCCACCCAAGGTGAAATAGTCAATGATGTTTTATTTTCAACCATTTCTTGCAAATTTAGATCATATTTTGGTTTTCTTTTAACTTTTGGCGAATTCTTATTTTTAGACTTTTTTTTTTTACTTATTAGAAATATCCAAATAATCCTTGGAAAAAAAAATATCAAAATAGATACGGCGAGAACTAGAGGTATTCATAGTGCATTTTGGTTTGGTTTTGTTTGATTTTGAGGTTAAAAAGTCATATTAACCGCAAGATAAAAAAAACATGGAGTTTAATTTGGTTCGGTTGACTTTTAAAATGAAAATCAAATGAAACAAATGCGGTTTGGTTTGGTTGTTACGGTTTTTTGCGACACAATTTTTTTGTGTTTAGCTCGGTTGCTAAAATAAAAAACCGCAAATTGAACTGAACCATGCGGCTGAGTAAAAAAAAGAAGATCCAAATACATCTGAATCAAATGTCGTTTTTGCAGTTTCAATTTAGATTAGTTCGGTTCGCGGTTTTTCTATTGTGTTGGTTTGGTTTTGAACACCCCCAAATGAGAACAACACAACTATTCATAGAAGACTGAAGAAAACACAACCATGAAACACAATCGTTTAGTTTGGGTCTACCTTTGCAACTAAAGCGGATTTTTTTTTTTGAAAATTTTTGTACAAAAAATTATCTTGTTTAAATGTTTATGAGAAAATAAAATAAAAATTATTGATTAAAAGATATTATTTTTTGCTCTAATTAACTCAACTTTTTTTCTTACACGAGAAAATTAACAACTGACTTAGAATCTCCACTATAAGTCCTAAGGATCCTAATTAATTACATAACTTGTTTTTTTCTTTTTCTTTTCCGTTGTTCTTTTGGGGTCTGCATGTAGGTCTTTCAAGGAAAGTCAAGTATGGTTTAAGCCTAGGTTTGGGAATACCTGGACTCCTAGGTCTAATTGTGTTTACATGGATGTTATGCAAAAAGAAGAACACAAGGAATCAAGTACAACGCCAAACAAGCACTGAATTTTCAACCTTTATTATGACGCACCCCCCAATTTTTGTTATGGGTCTTGACAGGGCAACAATCGAAAGATACCCTAAAACCCAGCTTGGTGAAAGTGGAAGATTGCCTAGGCCAAGTGACAACATTTGCTCTATATGTCTTTGTGAGTATCAGCCAAATGAAGTATTGAGGACAATTCCTGAGTGTAATCACTATTTTCATGTGAATTGTATAGATGGATGGCTCAAGACAAATGCTACTTGCCCTTTGTGTCGAAATTTTCCAGAGAGATCTACTTCGTTTTCTTCGGTTTTGCCTGTATCTCCAACTTCTTAA
Protein sequence

>MS.gene069590.t1

MNYSIVAMMSDPPFATPWTSLCEFISSALIPVEETNWLFWTDYYSNIPLQWDDPDCGNCEARGGRCGLVGDDALRLACYDLPTQGLSRKVKYGLSLGLGIPGLLGLIVFTWMLCKKKNTRNQVQRQTSTEFSTFIMTHPPIFVMGLDRATIERYPKTQLGESGRLPRPSDNICSICLCEYQPNEVLRTIPECNHYFHVNCIDGWLKTNATCPLCRNFPERSTSFSSVLPVSPTS