Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071034.t1 | KEH44502.1 | 65.5 | 139 | 23 | 2 | 12 | 125 | 4 | 142 | 4.50E-37 | 164.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071034.t1 | A0A072VRV0 | 65.5 | 139 | 23 | 2 | 12 | 125 | 4 | 142 | 3.2e-37 | 164.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene071034 | 0.814586 | 1.43E-51 | -1.69E-46 |
| MS.gene050828 | MS.gene071034 | 0.801953 | 7.06E-49 | -1.69E-46 |
| MS.gene051832 | MS.gene071034 | 0.815248 | 1.02E-51 | -1.69E-46 |
| MS.gene052685 | MS.gene071034 | 0.823657 | 1.23E-53 | -1.69E-46 |
| MS.gene05365 | MS.gene071034 | 0.80816 | 3.55E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene071034 | 0.807364 | 5.24E-50 | -1.69E-46 |
| MS.gene054222 | MS.gene071034 | 0.812956 | 3.27E-51 | -1.69E-46 |
| MS.gene05548 | MS.gene071034 | 0.800664 | 1.30E-48 | -1.69E-46 |
| MS.gene055841 | MS.gene071034 | 0.804928 | 1.71E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene071034 | 0.815268 | 1.01E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene071034 | 0.817813 | 2.71E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene071034 | -0.82141 | 4.10E-53 | -1.69E-46 |
| MS.gene059935 | MS.gene071034 | 0.807412 | 5.12E-50 | -1.69E-46 |
| MS.gene060365 | MS.gene071034 | 0.81525 | 1.02E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071034.t1 | MTR_1g115520 | 65.468 | 139 | 23 | 2 | 12 | 125 | 4 | 142 | 1.43e-55 | 170 |
| MS.gene071034.t1 | MTR_7g051400 | 37.126 | 167 | 48 | 2 | 1 | 110 | 253 | 419 | 4.08e-27 | 105 |
| MS.gene071034.t1 | MTR_1g105785 | 34.731 | 167 | 52 | 2 | 1 | 110 | 275 | 441 | 4.92e-23 | 93.6 |
| MS.gene071034.t1 | MTR_7g110100 | 31.548 | 168 | 56 | 3 | 1 | 109 | 275 | 442 | 6.28e-20 | 84.7 |
| MS.gene071034.t1 | MTR_1g492690 | 28.662 | 157 | 64 | 1 | 1 | 109 | 78 | 234 | 9.94e-18 | 77.8 |
| MS.gene071034.t1 | MTR_4g032585 | 29.885 | 174 | 63 | 3 | 1 | 115 | 19 | 192 | 3.53e-17 | 75.5 |
| MS.gene071034.t1 | MTR_4g032565 | 29.885 | 174 | 63 | 3 | 1 | 115 | 19 | 192 | 3.53e-17 | 75.5 |
| MS.gene071034.t1 | MTR_4g060433 | 79.545 | 44 | 9 | 0 | 1 | 44 | 19 | 62 | 6.46e-17 | 73.6 |
| MS.gene071034.t1 | MTR_8g104590 | 63.636 | 44 | 16 | 0 | 1 | 44 | 8 | 51 | 3.01e-14 | 66.2 |
| MS.gene071034.t1 | MTR_3g452170 | 34.862 | 109 | 57 | 2 | 1 | 109 | 275 | 369 | 3.75e-14 | 68.2 |
| MS.gene071034.t1 | MTR_8g064300 | 42.373 | 59 | 34 | 0 | 51 | 109 | 169 | 227 | 1.89e-11 | 60.1 |
| MS.gene071034.t1 | MTR_3g046650 | 33.333 | 93 | 36 | 1 | 1 | 67 | 110 | 202 | 3.68e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACACCATTCATTATATTAT+AGG | 0.291633 | 7.3:-34902305 | None:intergenic |
| TCAAGTGAATCTGATGTTCT+TGG | 0.373509 | 7.3:+34902230 | MS.gene071034:CDS |
| TATAAGCAAGCATAACCTTA+AGG | 0.380702 | 7.3:+34902119 | MS.gene071034:CDS |
| TTTGGTTTGCAGGCATGTCT+TGG | 0.387416 | 7.3:+34901512 | MS.gene071034:intron |
| CTGGTGTCGAATGGTATTTA+AGG | 0.443085 | 7.3:+34901567 | MS.gene071034:CDS |
| GACAATTGAAGATTTGATTG+AGG | 0.465748 | 7.3:+34901409 | MS.gene071034:CDS |
| AGACAAACAGACGTTGTTCA+AGG | 0.500909 | 7.3:+34902008 | MS.gene071034:CDS |
| ACCATTCATTATATTATAGG+TGG | 0.511076 | 7.3:-34902302 | None:intergenic |
| TAGGGAATGTGAATTATAAG+TGG | 0.530274 | 7.3:+34902043 | MS.gene071034:CDS |
| ACAGACGTTGTTCAAGGTGG+AGG | 0.574823 | 7.3:+34902014 | MS.gene071034:CDS |
| ACAATTTGCGAGATAGAGAC+TGG | 0.578498 | 7.3:+34901548 | MS.gene071034:CDS |
| TTCAAGGTGGAGGTTAGCAT+AGG | 0.581457 | 7.3:+34902024 | MS.gene071034:CDS |
| TCAAGGTGGAGGTTAGCATA+GGG | 0.582104 | 7.3:+34902025 | MS.gene071034:CDS |
| GGATTGCAGGCACGTAGTCA+AGG | 0.584383 | 7.3:+34901955 | MS.gene071034:intron |
| AGTCTCTATCTCGCAAATTG+TGG | 0.586282 | 7.3:-34901546 | None:intergenic |
| CCATTCATTATATTATAGGT+GGG | 0.597709 | 7.3:-34902301 | None:intergenic |
| AGATAGAGACTGGTGTCGAA+TGG | 0.611331 | 7.3:+34901558 | MS.gene071034:CDS |
| AGTGAATCTGATGTTCTTGG+TGG | 0.615328 | 7.3:+34902233 | MS.gene071034:CDS |
| GGAATGTGAATTATAAGTGG+AGG | 0.629082 | 7.3:+34902046 | MS.gene071034:CDS |
| CAAACAGACGTTGTTCAAGG+TGG | 0.667936 | 7.3:+34902011 | MS.gene071034:CDS |
| ATGCACGTCATTAGTGACTG+TGG | 0.672817 | 7.3:+34901601 | MS.gene071034:CDS |
| AGTCAAGAAAGCTACAGCTG+AGG | 0.711734 | 7.3:+34902077 | MS.gene071034:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAACTATGATACAAACAAT+TGG | - | chr7.3:34901481-34901500 | None:intergenic | 20.0% |
| !!! | AATCTTTTTCAAAATGACAT+GGG | + | chr7.3:34901902-34901921 | MS.gene071034:intron | 20.0% |
| !!! | AATTAAACGAATTTTTTTGC+AGG | + | chr7.3:34901934-34901953 | MS.gene071034:intron | 20.0% |
| !!! | CGAATTGTTTCTAATTTTAT+CGG | + | chr7.3:34901817-34901836 | MS.gene071034:intron | 20.0% |
| !!! | TAATCTTTTTCAAAATGACA+TGG | + | chr7.3:34901901-34901920 | MS.gene071034:intron | 20.0% |
| !!! | TAGTTATAATGCAATCATTT+TGG | + | chr7.3:34901494-34901513 | MS.gene071034:intron | 20.0% |
| ! | AACACATCAAAAAAGAAATG+CGG | - | chr7.3:34902182-34902201 | None:intergenic | 25.0% |
| ! | TGAAAAAGTTGTTGAAAATC+AGG | + | chr7.3:34902271-34902290 | MS.gene071034:CDS | 25.0% |
| !! | GATATTTGAAATTGCATTGT+AGG | + | chr7.3:34901734-34901753 | MS.gene071034:intron | 25.0% |
| AAAAACATGTCAAATTCAGC+TGG | - | chr7.3:34901988-34902007 | None:intergenic | 30.0% | |
| ACACATCAAAAAAGAAATGC+GGG | - | chr7.3:34902181-34902200 | None:intergenic | 30.0% | |
| ACTATGATACAAACAATTGG+AGG | - | chr7.3:34901478-34901497 | None:intergenic | 30.0% | |
| GACAATTGAAGATTTGATTG+AGG | + | chr7.3:34901409-34901428 | MS.gene071034:CDS | 30.0% | |
| TAGGGAATGTGAATTATAAG+TGG | + | chr7.3:34902043-34902062 | MS.gene071034:CDS | 30.0% | |
| TATAAGCAAGCATAACCTTA+AGG | + | chr7.3:34902119-34902138 | MS.gene071034:CDS | 30.0% | |
| GAGTGCAAACGAACAAAAAT+AGG | - | chr7.3:34902159-34902178 | None:intergenic | 35.0% | |
| GGAATGTGAATTATAAGTGG+AGG | + | chr7.3:34902046-34902065 | MS.gene071034:CDS | 35.0% | |
| TCAAGTGAATCTGATGTTCT+TGG | + | chr7.3:34902230-34902249 | MS.gene071034:CDS | 35.0% | |
| TCATTAGTGACTGTGGTTTA+TGG | + | chr7.3:34901608-34901627 | MS.gene071034:intron | 35.0% | |
| !!! | ATGCAATCATTTTGGTTTGC+AGG | + | chr7.3:34901502-34901521 | MS.gene071034:intron | 35.0% |
| !!! | GAATTTTTTTGCAGGATTGC+AGG | + | chr7.3:34901942-34901961 | MS.gene071034:intron | 35.0% |
| AATGCAAGAGTGATGTGCTA+AGG | + | chr7.3:34901657-34901676 | MS.gene071034:intron | 40.0% | |
| ACAATTTGCGAGATAGAGAC+TGG | + | chr7.3:34901548-34901567 | MS.gene071034:CDS | 40.0% | |
| AGACAAACAGACGTTGTTCA+AGG | + | chr7.3:34902008-34902027 | MS.gene071034:CDS | 40.0% | |
| AGTCTCTATCTCGCAAATTG+TGG | - | chr7.3:34901549-34901568 | None:intergenic | 40.0% | |
| AGTGAATCTGATGTTCTTGG+TGG | + | chr7.3:34902233-34902252 | MS.gene071034:CDS | 40.0% | |
| GAAGATCTGCTTGAGGTATA+TGG | + | chr7.3:34901850-34901869 | MS.gene071034:intron | 40.0% | |
| TAGCACATCACTCTTGCATT+GGG | - | chr7.3:34901657-34901676 | None:intergenic | 40.0% | |
| TCGATCGAACAACACAAATG+TGG | - | chr7.3:34901800-34901819 | None:intergenic | 40.0% | |
| TTAGCACATCACTCTTGCAT+TGG | - | chr7.3:34901658-34901677 | None:intergenic | 40.0% | |
| TTCTGTTGAAGATCTGCTTG+AGG | + | chr7.3:34901843-34901862 | MS.gene071034:intron | 40.0% | |
| ! | ACGTGTTTTCATATGCTGAC+AGG | + | chr7.3:34902205-34902224 | MS.gene071034:intron | 40.0% |
| ! | CTGGTGTCGAATGGTATTTA+AGG | + | chr7.3:34901567-34901586 | MS.gene071034:CDS | 40.0% |
| AGTCAAGAAAGCTACAGCTG+AGG | + | chr7.3:34902077-34902096 | MS.gene071034:CDS | 45.0% | |
| ATGCACGTCATTAGTGACTG+TGG | + | chr7.3:34901601-34901620 | MS.gene071034:CDS | 45.0% | |
| CAAACAGACGTTGTTCAAGG+TGG | + | chr7.3:34902011-34902030 | MS.gene071034:CDS | 45.0% | |
| GTGCGCGTTTGTAAACCTTA+AGG | - | chr7.3:34902137-34902156 | None:intergenic | 45.0% | |
| TCAAGGTGGAGGTTAGCATA+GGG | + | chr7.3:34902025-34902044 | MS.gene071034:CDS | 45.0% | |
| TTCAAGGTGGAGGTTAGCAT+AGG | + | chr7.3:34902024-34902043 | MS.gene071034:CDS | 45.0% | |
| ! | AGATAGAGACTGGTGTCGAA+TGG | + | chr7.3:34901558-34901577 | MS.gene071034:CDS | 45.0% |
| !! | TTTGGTTTGCAGGCATGTCT+TGG | + | chr7.3:34901512-34901531 | MS.gene071034:intron | 45.0% |
| ACAGACGTTGTTCAAGGTGG+AGG | + | chr7.3:34902014-34902033 | MS.gene071034:CDS | 50.0% | |
| GGATTGCAGGCACGTAGTCA+AGG | + | chr7.3:34901955-34901974 | MS.gene071034:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 34901407 | 34902316 | 34901407 | ID=MS.gene071034 |
| chr7.3 | mRNA | 34901407 | 34902316 | 34901407 | ID=MS.gene071034.t1;Parent=MS.gene071034 |
| chr7.3 | exon | 34901407 | 34901439 | 34901407 | ID=MS.gene071034.t1.exon1;Parent=MS.gene071034.t1 |
| chr7.3 | CDS | 34901407 | 34901439 | 34901407 | ID=cds.MS.gene071034.t1;Parent=MS.gene071034.t1 |
| chr7.3 | exon | 34901524 | 34901622 | 34901524 | ID=MS.gene071034.t1.exon2;Parent=MS.gene071034.t1 |
| chr7.3 | CDS | 34901524 | 34901622 | 34901524 | ID=cds.MS.gene071034.t1;Parent=MS.gene071034.t1 |
| chr7.3 | exon | 34901964 | 34902140 | 34901964 | ID=MS.gene071034.t1.exon3;Parent=MS.gene071034.t1 |
| chr7.3 | CDS | 34901964 | 34902140 | 34901964 | ID=cds.MS.gene071034.t1;Parent=MS.gene071034.t1 |
| chr7.3 | exon | 34902227 | 34902316 | 34902227 | ID=MS.gene071034.t1.exon4;Parent=MS.gene071034.t1 |
| chr7.3 | CDS | 34902227 | 34902316 | 34902227 | ID=cds.MS.gene071034.t1;Parent=MS.gene071034.t1 |
| Gene Sequence |
| Protein sequence |
