AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071556


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071556.t1 MTR_4g061010 97.458 118 3 0 1 118 54 171 3.73e-81 239
MS.gene071556.t1 MTR_6g033335 46.610 118 57 1 1 118 53 164 2.57e-31 112
MS.gene071556.t1 MTR_4g129670 47.458 118 60 1 1 118 53 168 3.35e-31 111
MS.gene071556.t1 MTR_1g095530 44.068 118 60 1 1 118 53 164 6.24e-23 90.5
MS.gene071556.t1 MTR_8g066790 39.474 114 65 1 5 118 57 166 7.92e-21 84.7
MS.gene071556.t1 MTR_8g097280 29.915 117 78 1 2 118 55 167 4.29e-18 77.8
MS.gene071556.t1 MTR_4g112570 36.735 98 62 0 1 98 56 153 3.11e-17 75.1
MS.gene071556.t1 MTR_8g101600 39.316 117 67 1 2 118 54 166 3.34e-16 72.4
MS.gene071556.t1 MTR_5g030070 40.367 109 61 1 10 118 62 166 3.31e-15 69.7
MS.gene071556.t1 MTR_2g056130 35.484 93 60 0 2 94 54 146 1.25e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071556.t1 AT3G45600 77.119 118 27 0 1 118 54 171 2.06e-66 199
MS.gene071556.t1 AT3G45600 77.119 118 27 0 1 118 54 171 3.81e-65 199
MS.gene071556.t1 AT5G60220 65.217 115 40 0 4 118 57 171 1.04e-49 160
MS.gene071556.t1 AT2G23810 44.915 118 63 1 1 118 54 169 7.42e-30 108
MS.gene071556.t1 AT3G12090 44.915 118 59 2 1 118 53 164 8.88e-26 97.8
MS.gene071556.t1 AT4G30430 45.263 95 52 0 1 95 54 148 2.93e-24 93.6
MS.gene071556.t1 AT4G28050 38.983 118 70 1 1 118 54 169 1.89e-23 91.3
MS.gene071556.t1 AT5G46700 41.379 116 58 3 6 118 58 166 3.74e-21 85.5
MS.gene071556.t1 AT1G63260 32.479 117 75 1 2 118 55 167 2.95e-20 82.0
MS.gene071556.t1 AT1G63260 32.479 117 75 1 2 118 55 167 4.74e-20 81.6
MS.gene071556.t1 AT1G63260 32.479 117 75 1 2 118 55 167 9.74e-20 82.0
MS.gene071556.t1 AT1G63260 32.479 117 75 1 2 118 55 167 9.74e-20 82.0
MS.gene071556.t1 AT1G63260 32.479 117 75 1 2 118 55 167 1.13e-19 81.3
MS.gene071556.t1 AT1G63260 32.479 117 75 1 2 118 55 167 1.15e-19 81.6
MS.gene071556.t1 AT1G18520 37.607 117 70 2 2 118 56 169 3.52e-19 80.5
MS.gene071556.t1 AT2G19580 40.517 116 64 2 4 118 56 167 8.16e-17 73.9
MS.gene071556.t1 AT4G23410 34.783 115 69 1 4 118 56 164 1.74e-16 73.2

Find 0 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATTTGCTTATGTTGTGA+CGG + 33285:8566-8585 MS.gene071556:CDS 25.0%
! GTATTTGGATTATTATTTGC+AGG + 33285:8623-8642 MS.gene071556:CDS 25.0%
! TATTATTTGCAGGATTATTC+TGG + 33285:8633-8652 MS.gene071556:CDS 25.0%
!! GAAGATTAGTTCTTGTATAA+GGG + 33285:8695-8714 MS.gene071556:CDS 25.0%
! GGAAGATTAGTTCTTGTATA+AGG + 33285:8694-8713 MS.gene071556:CDS 30.0%
!! AACGTAACAAGAAAGTGTTA+CGG - 33285:8497-8516 MS.gene071556:intergenic 30.0%
ATTTGCAGGATTATTCTGGT+TGG + 33285:8637-8656 MS.gene071556:CDS 35.0%
GAAGAAGGGTTATGAATAGA+GGG + 33285:8601-8620 MS.gene071556:CDS 35.0%
GTTGTCATGTTCTTAGTCAT+TGG + 33285:8525-8544 MS.gene071556:CDS 35.0%
TGACTAAGAACATGACAACA+AGG - 33285:8524-8543 MS.gene071556:intergenic 35.0%
TTAGTCATTGGTGTGCTTAT+TGG + 33285:8537-8556 MS.gene071556:CDS 35.0%
!! CTTATGTTGTGACGGATAAA+GGG + 33285:8574-8593 MS.gene071556:CDS 35.0%
!! GGTTATGAATAGAGGGTATT+TGG + 33285:8608-8627 MS.gene071556:CDS 35.0%
!! TAAGGGATTCTAAAGCTTGT+AGG + 33285:8712-8731 MS.gene071556:CDS 35.0%
!! TTCTAAAGCTTGTAGGAAGT+TGG + 33285:8719-8738 MS.gene071556:CDS 35.0%
AAGAACATATCAGCAGTCTC+AGG - 33285:8765-8784 MS.gene071555:intergenic 40.0%
AGTGGCTAGTGATGAGTATT+GGG + 33285:8671-8690 MS.gene071556:CDS 40.0%
GGAAGAAGGGTTATGAATAG+AGG + 33285:8600-8619 MS.gene071556:CDS 40.0%
!! GCTTATGTTGTGACGGATAA+AGG + 33285:8573-8592 MS.gene071556:CDS 40.0%
!! TTTCATTAGCCGGTTTTGCA+GGG + 33285:8463-8482 MS.gene071556:CDS 40.0%
AAGCTTGTAGGAAGTTGGCT+AGG + 33285:8724-8743 MS.gene071556:CDS 45.0%
GAGTGGCTAGTGATGAGTAT+TGG + 33285:8670-8689 MS.gene071556:CDS 45.0%
GGATAAAGGGTCTGGAAGAA+GGG + 33285:8587-8606 MS.gene071556:CDS 45.0%
! GCAGGATTATTCTGGTTGGT+TGG + 33285:8641-8660 MS.gene071556:CDS 45.0%
! GTGGCTAGTGATGAGTATTG+GGG + 33285:8672-8691 MS.gene071556:CDS 45.0%
!! GTTTCATTAGCCGGTTTTGC+AGG + 33285:8462-8481 MS.gene071556:CDS 45.0%
!! TGGCTAGTGATGAGTATTGG+GGG + 33285:8673-8692 MS.gene071556:CDS 45.0%
!! TTCATTAGCCGGTTTTGCAG+GGG + 33285:8464-8483 MS.gene071556:CDS 45.0%
CGGATAAAGGGTCTGGAAGA+AGG + 33285:8586-8605 MS.gene071556:CDS 50.0%
! TGGTTGGTTGGAAGAGAGAG+TGG + 33285:8653-8672 MS.gene071556:CDS 50.0%
!! GTTGTGACGGATAAAGGGTC+TGG + 33285:8579-8598 MS.gene071556:CDS 50.0%
GTAACACGCCCCTGCAAAAC+CGG - 33285:8475-8494 MS.gene071556:intergenic 55.0%


Chromosome Type Strat End Strand Name
33285 gene 8456 8809 8456 ID=MS.gene071556
33285 mRNA 8456 8809 8456 ID=MS.gene071556.t1;Parent=MS.gene071556
33285 exon 8456 8809 8456 ID=MS.gene071556.t1.exon1;Parent=MS.gene071556.t1
33285 CDS 8456 8809 8456 ID=cds.MS.gene071556.t1;Parent=MS.gene071556.t1
Gene Sequence

>MS.gene071556

ATGGTTGTTTCATTAGCCGGTTTTGCAGGGGCGTGTTACCGTAACACTTTCTTGTTACGTTTCTACCTTGTTGTCATGTTCTTAGTCATTGGTGTGCTTATTGGATTTATAATATTTGCTTATGTTGTGACGGATAAAGGGTCTGGAAGAAGGGTTATGAATAGAGGGTATTTGGATTATTATTTGCAGGATTATTCTGGTTGGTTGGAAGAGAGAGTGGCTAGTGATGAGTATTGGGGGAAGATTAGTTCTTGTATAAGGGATTCTAAAGCTTGTAGGAAGTTGGCTAGGAATTTTAATGATGTTCCTGAGACTGCTGATATGTTCTTTCAAAGAAAACTCAATCCTATTCAG

Protein sequence

>MS.gene071556.t1

MVVSLAGFAGACYRNTFLLRFYLVVMFLVIGVLIGFIIFAYVVTDKGSGRRVMNRGYLDYYLQDYSGWLEERVASDEYWGKISSCIRDSKACRKLARNFNDVPETADMFFQRKLNPIQ