AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07162

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07162.t1 MTR_1g088995 95.513 312 7 2 1 305 1 312 0.0 597
MS.gene07162.t1 MTR_1g088995 89.030 237 19 2 1 230 1 237 5.83e-141 397
MS.gene07162.t1 MTR_1g090330 34.146 287 178 4 29 305 17 302 3.14e-54 179
MS.gene07162.t1 MTR_1g090330 28.718 195 126 5 18 201 104 296 2.14e-14 72.8
MS.gene07162.t1 MTR_7g066690 36.545 301 163 6 16 298 7 297 2.23e-51 171
MS.gene07162.t1 MTR_7g066690 27.778 180 120 4 34 207 129 304 1.63e-12 67.4
MS.gene07162.t1 MTR_4g018750 34.194 310 169 6 10 300 4 297 4.77e-51 171
MS.gene07162.t1 MTR_3g100570 33.553 304 172 4 26 299 6 309 1.91e-50 169
MS.gene07162.t1 MTR_3g462140 34.915 295 169 7 21 299 10 297 6.31e-44 152
MS.gene07162.t1 MTR_5g032420 30.351 313 179 6 26 299 6 318 5.93e-41 145
MS.gene07162.t1 MTR_4g018750 32.971 276 150 6 10 266 4 263 8.18e-41 144
MS.gene07162.t1 MTR_8g068890 31.046 306 179 5 26 299 6 311 1.43e-38 138
MS.gene07162.t1 MTR_8g086070 28.479 309 188 10 9 299 2 295 1.08e-29 114
MS.gene07162.t1 MTR_4g057850 29.348 276 162 8 41 296 37 299 5.64e-23 97.1
MS.gene07162.t1 MTR_1g097890 29.630 270 162 8 33 299 59 303 5.52e-20 88.6
MS.gene07162.t1 MTR_1g097890 29.670 182 120 3 25 204 131 306 3.68e-12 66.2
MS.gene07162.t1 MTR_7g109135 31.000 300 172 13 22 299 19 305 8.72e-20 88.2
MS.gene07162.t1 MTR_7g109135 29.341 167 105 4 118 274 18 181 5.83e-11 62.8
MS.gene07162.t1 MTR_8g076760 27.208 283 181 9 41 302 29 307 9.00e-20 88.2
MS.gene07162.t1 MTR_2g436930 25.407 307 180 9 30 294 19 318 1.55e-19 87.8
MS.gene07162.t1 MTR_2g436930 27.933 179 103 5 117 274 10 183 2.42e-11 63.9
MS.gene07162.t1 MTR_8g086070 29.680 219 123 8 9 210 2 206 9.45e-18 80.5
MS.gene07162.t1 MTR_5g030530 27.208 283 180 8 39 301 21 297 1.58e-17 81.6
MS.gene07162.t1 MTR_1g095780 26.429 280 169 8 30 298 529 782 1.90e-17 83.2
MS.gene07162.t1 MTR_8g092080 28.016 257 158 7 21 274 47 279 1.91e-17 81.6
MS.gene07162.t1 MTR_4g119770 25.402 311 181 10 30 296 18 321 2.51e-17 81.3
MS.gene07162.t1 MTR_5g055410 28.889 270 176 8 42 298 37 303 1.03e-16 79.7
MS.gene07162.t1 MTR_7g109135 29.730 296 174 13 22 299 19 298 2.12e-16 78.6
MS.gene07162.t1 MTR_7g084400 26.705 176 123 3 30 202 313 485 2.43e-16 79.3
MS.gene07162.t1 MTR_7g084400 25.773 291 194 8 23 303 210 488 7.46e-14 72.0
MS.gene07162.t1 MTR_2g102010 26.592 267 175 7 20 274 11 268 3.31e-16 77.8
MS.gene07162.t1 MTR_4g114320 29.670 182 117 4 27 204 222 396 3.91e-16 78.6
MS.gene07162.t1 MTR_4g114320 24.815 270 192 5 34 299 131 393 3.60e-13 69.7
MS.gene07162.t1 MTR_4g114320 28.571 182 122 3 119 298 120 295 6.32e-12 65.9
MS.gene07162.t1 MTR_3g025800 24.556 338 206 11 1 298 1 329 4.22e-16 78.2
MS.gene07162.t1 MTR_1g087840 29.670 273 172 8 42 299 35 302 6.73e-16 77.0
MS.gene07162.t1 MTR_7g064980 23.860 285 202 5 30 299 29 313 8.55e-16 77.0
MS.gene07162.t1 MTR_7g064980 29.798 198 113 8 121 299 24 214 2.45e-13 69.7
MS.gene07162.t1 MTR_7g064980 26.168 214 126 8 12 207 120 319 3.76e-12 66.2
MS.gene07162.t1 MTR_3g107510 28.571 182 118 4 27 203 220 394 8.64e-16 77.4
MS.gene07162.t1 MTR_3g107510 25.000 288 201 8 17 299 115 392 8.96e-15 74.7
MS.gene07162.t1 MTR_8g096050 25.952 289 195 8 25 303 226 505 8.94e-16 77.8
MS.gene07162.t1 MTR_8g096050 26.500 200 138 4 9 202 306 502 1.32e-13 71.2
MS.gene07162.t1 MTR_1g012030 25.087 287 202 7 17 299 132 409 1.93e-15 76.6
MS.gene07162.t1 MTR_1g012030 28.283 198 128 5 11 203 223 411 1.25e-14 74.3
MS.gene07162.t1 MTR_3g098460 25.175 286 201 6 25 303 204 483 2.39e-15 76.6
MS.gene07162.t1 MTR_8g066975 26.690 281 184 7 39 301 21 297 3.37e-15 74.7
MS.gene07162.t1 MTR_7g109135 30.769 234 141 8 80 299 1 227 5.11e-15 73.6
MS.gene07162.t1 MTR_8g027975 29.442 197 127 4 110 297 41 234 5.34e-15 75.1
MS.gene07162.t1 MTR_8g027975 27.749 191 115 4 35 202 163 353 4.18e-14 72.4
MS.gene07162.t1 MTR_7g109135 33.333 174 107 5 133 299 35 206 8.04e-15 72.4
MS.gene07162.t1 MTR_4g012850 27.037 270 173 8 25 281 15 273 3.30e-14 72.0
MS.gene07162.t1 MTR_4g012850 28.659 164 105 4 42 196 126 286 7.20e-11 62.4
MS.gene07162.t1 MTR_7g068340 24.074 270 184 6 38 299 99 355 4.58e-14 72.4
MS.gene07162.t1 MTR_4g094578 23.934 305 186 10 23 302 22 305 1.09e-13 70.9
MS.gene07162.t1 MTR_2g032610 25.436 287 184 10 10 274 15 293 1.27e-13 70.5
MS.gene07162.t1 MTR_5g009500 27.513 189 116 4 35 202 150 338 1.73e-13 70.5
MS.gene07162.t1 MTR_5g009500 28.108 185 123 3 122 297 38 221 4.74e-13 68.9
MS.gene07162.t1 MTR_5g009500 22.050 322 213 8 16 303 24 341 1.40e-11 64.7
MS.gene07162.t1 MTR_3g104600 23.313 326 214 9 4 302 15 331 2.10e-13 70.1
MS.gene07162.t1 MTR_6g033280 25.217 230 146 6 24 234 57 279 4.19e-13 69.3
MS.gene07162.t1 MTR_5g073040 28.821 229 147 6 25 243 34 256 6.72e-13 68.6
MS.gene07162.t1 MTR_6g034515 24.911 281 174 10 41 298 82 348 1.24e-12 68.2
MS.gene07162.t1 MTR_6g091800 24.607 191 130 5 121 298 18 207 4.92e-12 65.5
MS.gene07162.t1 MTR_6g091800 24.607 191 130 5 121 298 18 207 5.16e-12 65.5
MS.gene07162.t1 MTR_6g091800 23.413 252 162 7 16 242 2 247 9.96e-11 61.6
MS.gene07162.t1 MTR_4g088545 22.295 305 209 6 20 303 6 303 1.15e-11 64.7
MS.gene07162.t1 MTR_7g015870 25.521 192 109 2 36 204 136 316 1.34e-11 64.7
MS.gene07162.t1 MTR_8g099910 24.675 231 146 8 24 234 67 289 1.71e-11 64.7
MS.gene07162.t1 MTR_6g015860 25.521 192 109 2 36 204 128 308 3.41e-11 63.5
MS.gene07162.t1 MTR_4g119770 24.701 251 146 8 30 244 18 261 3.87e-11 62.8
MS.gene07162.t1 MTR_6g091800 23.715 253 162 7 16 243 2 248 5.70e-11 62.8
MS.gene07162.t1 MTR_7g015870 25.397 189 107 2 36 201 132 309 7.03e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07162.t1 AT4G03115 69.307 303 88 2 3 305 17 314 2.05e-148 419
MS.gene07162.t1 AT4G03115 69.307 303 88 2 3 305 49 346 7.68e-148 419
MS.gene07162.t1 AT4G03115 72.047 254 68 1 52 305 1 251 1.33e-130 372
MS.gene07162.t1 AT4G03115 72.385 239 63 1 67 305 8 243 1.18e-123 353
MS.gene07162.t1 AT4G03115 69.444 216 61 2 3 218 17 227 2.55e-99 291
MS.gene07162.t1 AT4G03115 70.093 214 59 2 3 216 17 225 5.01e-99 291
MS.gene07162.t1 AT3G54110 33.898 295 177 5 17 298 6 295 5.26e-49 166
MS.gene07162.t1 AT5G58970 34.130 293 163 8 26 299 16 297 8.06e-46 157
MS.gene07162.t1 AT5G58970 28.090 178 122 3 31 204 125 300 5.62e-15 74.3
MS.gene07162.t1 AT1G14140 31.034 290 184 4 29 305 19 305 2.36e-43 150
MS.gene07162.t1 AT4G24570 30.476 315 167 6 26 299 6 309 1.00e-42 149
MS.gene07162.t1 AT5G09470 30.211 331 171 6 23 297 3 329 1.65e-37 136
MS.gene07162.t1 AT2G22500 29.967 307 177 7 26 299 6 307 2.90e-34 127
MS.gene07162.t1 AT5G58970 33.333 240 136 7 26 246 16 250 1.26e-32 122
MS.gene07162.t1 AT5G58970 27.500 160 104 3 127 274 21 180 6.24e-11 62.4
MS.gene07162.t1 AT5G19760 28.428 299 186 9 17 299 6 292 1.92e-29 114
MS.gene07162.t1 AT5G01340 30.952 294 164 9 27 296 17 295 5.83e-25 102
MS.gene07162.t1 AT5G48970 27.036 307 176 10 31 294 27 328 5.89e-20 89.0
MS.gene07162.t1 AT5G66380 27.397 292 191 9 27 299 10 299 2.51e-19 86.7
MS.gene07162.t1 AT5G66380 25.778 225 139 9 9 210 89 308 1.99e-11 63.9
MS.gene07162.t1 AT3G53940 26.689 296 194 8 25 299 66 359 2.62e-19 87.4
MS.gene07162.t1 AT3G53940 28.077 260 150 8 69 297 5 258 9.81e-15 74.3
MS.gene07162.t1 AT3G53940 25.000 188 125 4 25 202 179 360 8.71e-11 62.4
MS.gene07162.t1 AT2G26360 26.882 279 171 8 30 298 119 374 1.86e-18 85.1
MS.gene07162.t1 AT1G07030 29.278 263 167 9 23 274 36 290 4.73e-18 83.6
MS.gene07162.t1 AT2G37890 24.662 296 198 7 23 299 42 331 7.72e-18 82.8
MS.gene07162.t1 AT3G21390 26.488 336 187 12 7 294 23 346 3.05e-17 81.6
MS.gene07162.t1 AT3G21390 26.518 313 171 11 30 294 23 324 3.38e-17 81.3
MS.gene07162.t1 AT1G25380 27.715 267 176 9 42 294 37 300 4.46e-17 81.3
MS.gene07162.t1 AT1G25380 27.715 267 176 9 42 294 37 300 4.46e-17 81.3
MS.gene07162.t1 AT5G51050 30.114 176 118 2 30 202 322 495 8.79e-17 80.9
MS.gene07162.t1 AT5G51050 25.704 284 191 7 24 298 221 493 4.52e-15 75.9
MS.gene07162.t1 AT2G47490 29.710 276 167 9 42 299 33 299 3.15e-16 78.2
MS.gene07162.t1 AT2G35800 25.368 272 167 8 30 290 549 795 3.19e-16 79.7
MS.gene07162.t1 AT1G34065 25.267 281 185 7 21 299 70 327 3.80e-16 78.2
MS.gene07162.t1 AT1G34065 25.267 281 185 7 21 299 77 334 4.51e-16 78.2
MS.gene07162.t1 AT1G74240 25.879 313 184 10 22 297 32 333 8.53e-16 77.4
MS.gene07162.t1 AT1G74240 25.253 198 124 6 125 299 39 235 7.16e-11 62.8
MS.gene07162.t1 AT4G01100 26.131 199 121 4 27 202 99 294 1.14e-15 76.3
MS.gene07162.t1 AT4G01100 23.129 294 190 8 42 303 8 297 1.93e-11 63.9
MS.gene07162.t1 AT3G55640 25.263 285 192 7 30 297 42 322 1.29e-15 76.6
MS.gene07162.t1 AT3G55640 29.319 191 117 5 121 297 37 223 4.60e-15 75.1
MS.gene07162.t1 AT3G55640 25.263 285 192 7 30 297 42 322 1.29e-15 76.6
MS.gene07162.t1 AT3G55640 29.319 191 117 5 121 297 37 223 4.60e-15 75.1
MS.gene07162.t1 AT4G01100 26.131 199 121 4 27 202 163 358 1.63e-15 76.6
MS.gene07162.t1 AT4G01100 23.256 344 210 10 10 303 22 361 4.37e-13 69.3
MS.gene07162.t1 AT3G55640 29.319 191 117 5 121 297 37 223 1.78e-15 75.1
MS.gene07162.t1 AT3G55640 29.319 191 117 5 121 297 37 223 1.78e-15 75.1
MS.gene07162.t1 AT4G01100 26.131 199 121 4 27 202 149 344 2.19e-15 76.3
MS.gene07162.t1 AT4G01100 23.030 330 214 9 10 303 22 347 8.93e-14 71.2
MS.gene07162.t1 AT4G39460 26.275 255 165 5 21 274 53 285 2.77e-15 75.5
MS.gene07162.t1 AT4G39460 26.275 255 165 5 21 274 53 285 2.77e-15 75.5
MS.gene07162.t1 AT4G39460 26.275 255 165 5 21 274 53 285 2.77e-15 75.5
MS.gene07162.t1 AT3G53940 28.077 260 150 8 69 297 5 258 4.30e-15 74.7
MS.gene07162.t1 AT5G46800 28.269 283 172 9 39 297 21 296 4.41e-15 74.7
MS.gene07162.t1 AT5G46800 26.154 195 128 3 121 299 7 201 1.84e-12 67.0
MS.gene07162.t1 AT5G46800 28.269 283 172 9 39 297 21 296 4.41e-15 74.7
MS.gene07162.t1 AT5G46800 26.154 195 128 3 121 299 7 201 1.84e-12 67.0
MS.gene07162.t1 AT5G46800 28.269 283 172 9 39 297 21 296 4.41e-15 74.7
MS.gene07162.t1 AT5G46800 26.154 195 128 3 121 299 7 201 1.84e-12 67.0
MS.gene07162.t1 AT5G61810 28.249 177 119 4 30 202 160 332 5.97e-15 74.7
MS.gene07162.t1 AT4G26180 21.698 318 207 8 8 297 3 306 8.82e-15 73.9
MS.gene07162.t1 AT5G61810 28.249 177 119 4 30 202 303 475 1.18e-14 74.3
MS.gene07162.t1 AT4G26180 23.256 258 168 6 8 244 3 251 1.50e-14 73.2
MS.gene07162.t1 AT1G79900 29.940 167 106 5 34 196 118 277 3.15e-14 72.0
MS.gene07162.t1 AT1G79900 26.693 251 157 8 41 281 31 264 3.89e-14 72.0
MS.gene07162.t1 AT2G30160 25.357 280 190 8 34 299 46 320 6.43e-14 71.6
MS.gene07162.t1 AT4G32400 25.368 272 188 8 34 299 122 384 1.13e-13 71.2
MS.gene07162.t1 AT4G32400 28.161 174 114 4 37 204 219 387 1.47e-13 70.9
MS.gene07162.t1 AT1G74240 26.190 294 169 10 41 297 3 285 1.20e-13 70.9
MS.gene07162.t1 AT5G07320 27.011 174 121 3 30 200 304 474 1.43e-13 71.2
MS.gene07162.t1 AT5G48970 25.191 262 160 8 64 294 1 257 2.06e-13 69.3
MS.gene07162.t1 AT5G48970 26.570 207 123 6 26 205 64 268 5.49e-11 62.4
MS.gene07162.t1 AT2G39970 25.246 305 185 7 39 302 21 323 2.87e-13 69.7
MS.gene07162.t1 AT5G01500 25.641 273 176 8 38 299 131 387 5.48e-13 69.3
MS.gene07162.t1 AT3G51870 25.461 271 176 8 38 296 103 359 8.66e-13 68.6
MS.gene07162.t1 AT3G48850 26.829 246 151 8 25 251 66 301 2.91e-12 67.0
MS.gene07162.t1 AT1G14560 24.286 280 183 10 42 297 43 317 5.07e-12 65.9
MS.gene07162.t1 AT3G20240 22.896 297 200 6 24 299 50 338 6.84e-12 65.9

Find 91 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCGGGAATTCCCCACTTTC+AGG 0.234620 1.4:+70917094 MS.gene07162:CDS
AGGACCTAAGTCTTTATATC+TGG 0.241224 1.4:+70917943 MS.gene07162:CDS
TATCCATGGGAGCAGTTATA+AGG 0.254065 1.4:-70919374 None:intergenic
TTAACAAGGTCAGTTGTTTA+TGG 0.264230 1.4:+70917980 MS.gene07162:CDS
TGAACTACCATTCCACTTAA+AGG 0.268699 1.4:-70917810 None:intergenic
CAGGAGATGAGAAGCAAAAT+TGG 0.295563 1.4:+70917245 MS.gene07162:intron
CATGCATGTGATCTTGCTTT+TGG 0.305682 1.4:+70918037 MS.gene07162:CDS
TATAACTGCTCCCATGGATA+TGG 0.337685 1.4:+70919377 MS.gene07162:CDS
GCAGCGGCTCTGGCCATGGC+AGG 0.346797 1.4:-70918331 None:intergenic
TGCAGTCAAGGCAGCGGCTC+TGG 0.352494 1.4:-70918341 None:intergenic
ACCTGAAAGTGGGGAATTCC+CGG 0.362222 1.4:-70917095 None:intergenic
TACAGCTCAAGCACAGTTGC+AGG 0.372510 1.4:+70919339 MS.gene07162:intron
GGTCCAAGAGCACTTTATAA+AGG 0.396572 1.4:+70919790 MS.gene07162:CDS
CACCCTTTATAAAGTGCTCT+TGG 0.409133 1.4:-70919793 None:intergenic
GAAAACAGTGGAAGGGGACT+TGG 0.417299 1.4:-70917269 None:intergenic
ATTGCTTCGGGAATGTTTGC+TGG 0.424631 1.4:+70918082 MS.gene07162:CDS
CTTGAGAACTTCCATTGGAT+TGG 0.428326 1.4:-70918125 None:intergenic
GTCCTCCGGAGCTCTAAAAT+TGG 0.430225 1.4:-70918265 None:intergenic
GCTAAGGAAAATTGCTGGCT+TGG 0.440588 1.4:+70919974 MS.gene07162:CDS
TTTATATATTCTACCACCCT+TGG 0.448676 1.4:-70919430 None:intergenic
AAGATCACATGCATGCTTAG+AGG 0.450159 1.4:-70918029 None:intergenic
GGACCTAAGTCTTTATATCT+GGG 0.451211 1.4:+70917944 MS.gene07162:CDS
TTCACACTATTTGCAAGATT+AGG 0.452464 1.4:+70919915 MS.gene07162:CDS
TTCAATCACTTTGCAACAAG+TGG 0.457233 1.4:+70917289 MS.gene07162:CDS
ATCCATGGGAGCAGTTATAA+GGG 0.468553 1.4:-70919373 None:intergenic
GAGGAGCTAAGGAAAATTGC+TGG 0.470269 1.4:+70919969 MS.gene07162:CDS
AAGGAAAATTGCTGGCTTGG+AGG 0.471885 1.4:+70919977 MS.gene07162:CDS
GCAAAGTCGTAACCTGAAAG+TGG 0.477023 1.4:-70917106 None:intergenic
GAATCAAAGCTCTGTGGAAG+GGG 0.477216 1.4:+70918302 MS.gene07162:CDS
CAAGGCAGCGGCTCTGGCCA+TGG 0.478937 1.4:-70918335 None:intergenic
GTTTATGGAGGACTACGCTT+GGG 0.480206 1.4:+70917995 MS.gene07162:CDS
GTCCAAGAGCACTTTATAAA+GGG 0.488553 1.4:+70919791 MS.gene07162:CDS
GGTCAGAAGGGTCCTTTAAG+TGG 0.489706 1.4:+70917798 MS.gene07162:CDS
TTATATTATGTGAGGAGCTA+AGG 0.489794 1.4:+70919958 MS.gene07162:CDS
TTATGCAGATTCTAGTCAAA+TGG 0.493713 1.4:+70918492 MS.gene07162:intron
CTCACCTTGAGAACTTCCAT+TGG 0.501234 1.4:-70918130 None:intergenic
AGATAGAACAAAATAATGGC+CGG 0.505415 1.4:+70917076 None:intergenic
CAGCGGCTCTGGCCATGGCA+GGG 0.506305 1.4:-70918330 None:intergenic
AAACCCAGATATAAAGACTT+AGG 0.506639 1.4:-70917947 None:intergenic
AGGAGATGAGAAGCAAAATT+GGG 0.507075 1.4:+70917246 MS.gene07162:intron
ACTTTAGAAATTGACCTGCT+TGG 0.513231 1.4:-70918389 None:intergenic
TGGATTTAACTGTGCATACC+AGG 0.515008 1.4:+70919455 MS.gene07162:CDS
AAATGGACATCTCTCAAAGA+AGG 0.520401 1.4:+70918509 MS.gene07162:CDS
AGGCTACAAATGCAACTTGT+TGG 0.521186 1.4:+70917777 MS.gene07162:CDS
GGAATCAAAGCTCTGTGGAA+GGG 0.524389 1.4:+70918301 MS.gene07162:CDS
CACCCTTATAACTGCTCCCA+TGG 0.525209 1.4:+70919371 MS.gene07162:CDS
ACATAATATAAATGTAATTG+TGG 0.532518 1.4:-70919946 None:intergenic
TGCCTTGACTGCATCTCAGT+TGG 0.532941 1.4:+70918351 MS.gene07162:CDS
ATAAATGTAATTGTGGTTTG+TGG 0.535455 1.4:-70919939 None:intergenic
AGCCAACTGAGATGCAGTCA+AGG 0.539944 1.4:-70918353 None:intergenic
AGATCACATGCATGCTTAGA+GGG 0.540217 1.4:-70918028 None:intergenic
GTGATTGAAAACAGTGGAAG+GGG 0.540688 1.4:-70917275 None:intergenic
GAAGGGTCCTTTAAGTGGAA+TGG 0.541076 1.4:+70917803 MS.gene07162:CDS
GGGAATCAAAGCTCTGTGGA+AGG 0.543413 1.4:+70918300 MS.gene07162:CDS
TGTTTATGGAGGACTACGCT+TGG 0.543442 1.4:+70917994 MS.gene07162:CDS
GAGCAGTTATAAGGGTGCTC+AGG 0.543472 1.4:-70919365 None:intergenic
CATGTTGCAACGAGAATCCA+AGG 0.552643 1.4:+70919413 MS.gene07162:CDS
GATAGAACAAAATAATGGCC+GGG 0.553927 1.4:+70917077 None:intergenic
CAATCCAATGGAAGTTCTCA+AGG 0.555333 1.4:+70918126 MS.gene07162:CDS
ATGCAACTTGTTGGTCAGAA+GGG 0.561302 1.4:+70917786 MS.gene07162:CDS
CCTCTTCAGATATAGTCCTC+CGG 0.564864 1.4:-70918279 None:intergenic
AAGTGATTGAAAACAGTGGA+AGG 0.571611 1.4:-70917277 None:intergenic
AATGCAACTTGTTGGTCAGA+AGG 0.572639 1.4:+70917785 MS.gene07162:CDS
TACTTATGATGAAACCAAGC+AGG 0.576672 1.4:+70918375 MS.gene07162:CDS
CCGGAGGACTATATCTGAAG+AGG 0.578111 1.4:+70918279 MS.gene07162:CDS
CAAAGTCGTAACCTGAAAGT+GGG 0.581145 1.4:-70917105 None:intergenic
GTTTGACACCTGCTTTAACA+AGG 0.581556 1.4:+70917966 MS.gene07162:CDS
ACAACTGACCTTGTTAAAGC+AGG 0.582971 1.4:-70917974 None:intergenic
CTGAGATGCAGTCAAGGCAG+CGG 0.589986 1.4:-70918347 None:intergenic
ACATCAATTACTCATCCTCT+AGG 0.590063 1.4:+70917331 MS.gene07162:CDS
TTTCAGGTCCTACTTACAGA+GGG 0.596108 1.4:+70919769 MS.gene07162:intron
AATGAATTCAGACATGGGGA+AGG 0.600607 1.4:+70918237 MS.gene07162:CDS
ACAAGGTCAGTTGTTTATGG+AGG 0.600975 1.4:+70917983 MS.gene07162:CDS
CTCTTGGACCCTCTGTAAGT+AGG 0.603008 1.4:-70919777 None:intergenic
CGGAGGACTATATCTGAAGA+GGG 0.606262 1.4:+70918280 MS.gene07162:CDS
TGCAAATGAATTCAGACATG+GGG 0.616704 1.4:+70918233 MS.gene07162:CDS
TTTGCAAATGAATTCAGACA+TGG 0.617094 1.4:+70918231 MS.gene07162:CDS
AATTACATTTATATTATGTG+AGG 0.620137 1.4:+70919950 MS.gene07162:CDS
AATCAAAGCTCTGTGGAAGG+GGG 0.622362 1.4:+70918303 MS.gene07162:CDS
TTGCAAATGAATTCAGACAT+GGG 0.626451 1.4:+70918232 MS.gene07162:CDS
AACTGCATTCACCAATCCAA+TGG 0.630939 1.4:+70918114 MS.gene07162:CDS
GTGCAGATGTCTTGAAAGTG+AGG 0.638032 1.4:+70917757 MS.gene07162:intron
ATGAATTCAGACATGGGGAA+GGG 0.639473 1.4:+70918238 MS.gene07162:CDS
AGTGATTGAAAACAGTGGAA+GGG 0.640136 1.4:-70917276 None:intergenic
AATTCAGACATGGGGAAGGG+CGG 0.648781 1.4:+70918241 MS.gene07162:CDS
GTTTCAGGTCCTACTTACAG+AGG 0.649161 1.4:+70919768 MS.gene07162:intron
AAGAGGGAATCAAAGCTCTG+TGG 0.664897 1.4:+70918296 MS.gene07162:CDS
TGCAAAGTGATTGAAAACAG+TGG 0.674194 1.4:-70917281 None:intergenic
AAAGTCGTAACCTGAAAGTG+GGG 0.692819 1.4:-70917104 None:intergenic
TTGCAACGAGAATCCAAGGG+TGG 0.712682 1.4:+70919417 MS.gene07162:CDS
ATGTTGCAACGAGAATCCAA+GGG 0.732517 1.4:+70919414 MS.gene07162:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATTGAAAGTAAATTA+GGG - chr1.4:70918442-70918461 None:intergenic 10.0%
!! TTTCTAATAAAATACAATAA+GGG + chr1.4:70919047-70919066 MS.gene07162:intron 10.0%
!!! TTTTCTAATAAAATACAATA+AGG + chr1.4:70919046-70919065 MS.gene07162:intron 10.0%
!! AATTACATTTATATTATGTG+AGG + chr1.4:70919950-70919969 MS.gene07162:CDS 15.0%
!! ACATAATATAAATGTAATTG+TGG - chr1.4:70919949-70919968 None:intergenic 15.0%
!! ATTATTATAAAGAGAAAAAC+AGG - chr1.4:70917363-70917382 None:intergenic 15.0%
!! GATTTAATTGAAAGTAAATT+AGG - chr1.4:70918443-70918462 None:intergenic 15.0%
!! TGTTGTATATTATATAGTTA+GGG + chr1.4:70917424-70917443 MS.gene07162:intron 15.0%
!! TTGTTGTATATTATATAGTT+AGG + chr1.4:70917423-70917442 MS.gene07162:intron 15.0%
!!! ATTTTATTCTTATTCTTTCT+GGG + chr1.4:70917551-70917570 MS.gene07162:intron 15.0%
!!! GTTTTGTATTATAATGTAAT+AGG - chr1.4:70917866-70917885 None:intergenic 15.0%
!!! TATTTTATTCTTATTCTTTC+TGG + chr1.4:70917550-70917569 MS.gene07162:intron 15.0%
!! AAAGCTAATAACTAAGAATT+AGG - chr1.4:70919642-70919661 None:intergenic 20.0%
!! GTATTATAATGTAATAGGTA+AGG - chr1.4:70917861-70917880 None:intergenic 20.0%
!! GTTAAATAGCATTAAAGTTT+AGG - chr1.4:70919870-70919889 None:intergenic 20.0%
!! TAAAGATGTTATCATTAAGT+TGG + chr1.4:70917617-70917636 MS.gene07162:intron 20.0%
!! TCTTCTCAACATATTATTAA+AGG - chr1.4:70917660-70917679 None:intergenic 20.0%
!! TGAAAAATAATGAATGTATC+AGG + chr1.4:70917226-70917245 MS.gene07162:intron 20.0%
!!! AAAGAAGTATAGAACTTATA+TGG - chr1.4:70919590-70919609 None:intergenic 20.0%
!!! AGCAATTTTAACTAAAACAT+TGG - chr1.4:70918068-70918087 None:intergenic 20.0%
!!! ATATCATTGTTTTACATGAT+TGG - chr1.4:70919699-70919718 None:intergenic 20.0%
!!! GAGAAGAAACTATTTTATTT+GGG + chr1.4:70917673-70917692 MS.gene07162:intron 20.0%
!!! GTATTTTATTAGAAAACACA+AGG - chr1.4:70919042-70919061 None:intergenic 20.0%
!!! TATCATTGTTTTACATGATT+GGG - chr1.4:70919698-70919717 None:intergenic 20.0%
!!! TCTTAGTTATTAGCTTTTAA+TGG + chr1.4:70919645-70919664 MS.gene07162:intron 20.0%
!!! TGAGAAGAAACTATTTTATT+TGG + chr1.4:70917672-70917691 MS.gene07162:intron 20.0%
!!! TGTTTTAGTTAAAATTGCTT+CGG + chr1.4:70918069-70918088 MS.gene07162:CDS 20.0%
!!! TTTTTTTTTGCTTAATGTAC+AGG - chr1.4:70918171-70918190 None:intergenic 20.0%
! AAATGGGTCAAAGAAATAAA+AGG - chr1.4:70917133-70917152 None:intergenic 25.0%
! ATAAATGTAATTGTGGTTTG+TGG - chr1.4:70919942-70919961 None:intergenic 25.0%
! ATAAGAACACAAATTCTTCA+CGG - chr1.4:70918999-70919018 None:intergenic 25.0%
! ATGTTATGTTAGAGAGAAAT+GGG - chr1.4:70917149-70917168 None:intergenic 25.0%
! ATTACAATTACAACTTGTGA+TGG - chr1.4:70917190-70917209 None:intergenic 25.0%
! CAACAAGATATCAATCAAAT+TGG - chr1.4:70917409-70917428 None:intergenic 25.0%
! CTTCATTATTCTTCACTATA+TGG - chr1.4:70918663-70918682 None:intergenic 25.0%
! GGTGGTAGAATATATAAAAA+TGG + chr1.4:70919435-70919454 MS.gene07162:CDS 25.0%
! TGAGAAAACAATTTAATACC+TGG - chr1.4:70919476-70919495 None:intergenic 25.0%
! TTATATGAAACAATGTCAAG+AGG + chr1.4:70918933-70918952 MS.gene07162:intron 25.0%
! TTTCATCTACATCTTATGTA+TGG + chr1.4:70918533-70918552 MS.gene07162:intron 25.0%
!! ATAGTTAGTTTGACATTAGT+TGG + chr1.4:70918874-70918893 MS.gene07162:intron 25.0%
!! CAATGATATATTTTCTGATC+TGG + chr1.4:70919710-70919729 MS.gene07162:intron 25.0%
!! GAAACAATGATTTTCAACAT+TGG + chr1.4:70918813-70918832 MS.gene07162:intron 25.0%
!! TACATTCTTTAACTTTGACT+AGG + chr1.4:70919612-70919631 MS.gene07162:intron 25.0%
!! TAGTTAGTTTGACATTAGTT+GGG + chr1.4:70918875-70918894 MS.gene07162:intron 25.0%
!!! GTTTTAGTTAAAATTGCTTC+GGG + chr1.4:70918070-70918089 MS.gene07162:CDS 25.0%
AAACCCAGATATAAAGACTT+AGG - chr1.4:70917950-70917969 None:intergenic 30.0%
CAAAGATTGACCAAAATCAT+GGG + chr1.4:70918626-70918645 MS.gene07162:intron 30.0%
CAATTCCAACTGTTACAATT+AGG - chr1.4:70919076-70919095 None:intergenic 30.0%
CATGTTATGTTAGAGAGAAA+TGG - chr1.4:70917150-70917169 None:intergenic 30.0%
GATGATTTATTGATACATCG+AGG + chr1.4:70917509-70917528 MS.gene07162:intron 30.0%
TTAACAAGGTCAGTTGTTTA+TGG + chr1.4:70917980-70917999 MS.gene07162:CDS 30.0%
TTAAGTCACATCTCTGATAA+TGG - chr1.4:70919298-70919317 None:intergenic 30.0%
TTAAGTGTGGTGAAAAATGA+AGG + chr1.4:70917923-70917942 MS.gene07162:CDS 30.0%
TTATATTATGTGAGGAGCTA+AGG + chr1.4:70919958-70919977 MS.gene07162:CDS 30.0%
TTATGCAGATTCTAGTCAAA+TGG + chr1.4:70918492-70918511 MS.gene07162:intron 30.0%
TTCACACTATTTGCAAGATT+AGG + chr1.4:70919915-70919934 MS.gene07162:CDS 30.0%
TTGCAAATGAATTCAGACAT+GGG + chr1.4:70918232-70918251 MS.gene07162:CDS 30.0%
TTTATATATTCTACCACCCT+TGG - chr1.4:70919433-70919452 None:intergenic 30.0%
TTTGCAAATGAATTCAGACA+TGG + chr1.4:70918231-70918250 MS.gene07162:CDS 30.0%
! ATCCATTTAGAATTCATTGC+AGG - chr1.4:70919170-70919189 None:intergenic 30.0%
! GTCAATTAAGCATTTTTCCA+GGG + chr1.4:70917890-70917909 MS.gene07162:intron 30.0%
! TGTCAATTAAGCATTTTTCC+AGG + chr1.4:70917889-70917908 MS.gene07162:intron 30.0%
! TTAATGCTATTTAACATCCG+AGG + chr1.4:70919875-70919894 MS.gene07162:intron 30.0%
!! AAAGGGTGAGTTTTATGTTT+GGG + chr1.4:70919808-70919827 MS.gene07162:intron 30.0%
!! ACATCTCTGATAATGGAAAA+TGG - chr1.4:70919291-70919310 None:intergenic 30.0%
!! CATGATTTTGGTCAATCTTT+GGG - chr1.4:70918627-70918646 None:intergenic 30.0%
!! TAAAGGGTGAGTTTTATGTT+TGG + chr1.4:70919807-70919826 MS.gene07162:intron 30.0%
!!! CTCTGCTTCTTTATTTTAAG+TGG + chr1.4:70919535-70919554 MS.gene07162:intron 30.0%
AAATGGACATCTCTCAAAGA+AGG + chr1.4:70918509-70918528 MS.gene07162:CDS 35.0%
AAGTGATTGAAAACAGTGGA+AGG - chr1.4:70917280-70917299 None:intergenic 35.0%
ACATCAATTACTCATCCTCT+AGG + chr1.4:70917331-70917350 MS.gene07162:CDS 35.0%
AGCCTGCAATGAATTCTAAA+TGG + chr1.4:70919165-70919184 MS.gene07162:intron 35.0%
AGGACCTAAGTCTTTATATC+TGG + chr1.4:70917943-70917962 MS.gene07162:CDS 35.0%
AGGAGATGAGAAGCAAAATT+GGG + chr1.4:70917246-70917265 MS.gene07162:intron 35.0%
AGTCTGCATTAAGAGAGATA+TGG - chr1.4:70918581-70918600 None:intergenic 35.0%
AGTGATTGAAAACAGTGGAA+GGG - chr1.4:70917279-70917298 None:intergenic 35.0%
CACTTAAAAACAATTGTCCC+TGG - chr1.4:70917910-70917929 None:intergenic 35.0%
CCAAAGATTGACCAAAATCA+TGG + chr1.4:70918625-70918644 MS.gene07162:intron 35.0%
CTAATGAATTCAGACTTGTG+TGG - chr1.4:70919243-70919262 None:intergenic 35.0%
CTACTACTACTCATTAGTGA+TGG + chr1.4:70917472-70917491 MS.gene07162:intron 35.0%
CTTGTTGCAAATGCAAGATT+AGG + chr1.4:70919137-70919156 MS.gene07162:intron 35.0%
GAAAGTAAATTAGGGAGATG+AGG - chr1.4:70918434-70918453 None:intergenic 35.0%
GGAAACCTAATTGTAACAGT+TGG + chr1.4:70919068-70919087 MS.gene07162:intron 35.0%
GGACCTAAGTCTTTATATCT+GGG + chr1.4:70917944-70917963 MS.gene07162:CDS 35.0%
GTAATAGGTAAGGTCTAAAG+AGG - chr1.4:70917851-70917870 None:intergenic 35.0%
TACTTATGATGAAACCAAGC+AGG + chr1.4:70918375-70918394 MS.gene07162:CDS 35.0%
TGAACTACCATTCCACTTAA+AGG - chr1.4:70917813-70917832 None:intergenic 35.0%
TGCAAAGTGATTGAAAACAG+TGG - chr1.4:70917284-70917303 None:intergenic 35.0%
TGCAAATGAATTCAGACATG+GGG + chr1.4:70918233-70918252 MS.gene07162:CDS 35.0%
TTCAATCACTTTGCAACAAG+TGG + chr1.4:70917289-70917308 MS.gene07162:CDS 35.0%
TTGACATTAGTTGGGAACTT+GGG + chr1.4:70918883-70918902 MS.gene07162:intron 35.0%
TTTGACATTAGTTGGGAACT+TGG + chr1.4:70918882-70918901 MS.gene07162:intron 35.0%
! ACATCGAGGATAGATCTTTT+AGG + chr1.4:70917523-70917542 MS.gene07162:intron 35.0%
! ACTTTAGAAATTGACCTGCT+TGG - chr1.4:70918392-70918411 None:intergenic 35.0%
! ATTTAGAATTCATTGCAGGC+TGG - chr1.4:70919166-70919185 None:intergenic 35.0%
!! CCATGATTTTGGTCAATCTT+TGG - chr1.4:70918628-70918647 None:intergenic 35.0%
!! GTCCAAGAGCACTTTATAAA+GGG + chr1.4:70919791-70919810 MS.gene07162:CDS 35.0%
!! TAGAACAAACAAAGTACTGC+AGG - chr1.4:70919508-70919527 None:intergenic 35.0%
!!! ACGTGTTTTAACCATATCCA+TGG - chr1.4:70919391-70919410 None:intergenic 35.0%
!!! CGTGTTTTAACCATATCCAT+GGG - chr1.4:70919390-70919409 None:intergenic 35.0%
!!! GGGACAATTGTTTTTAAGTG+TGG + chr1.4:70917910-70917929 MS.gene07162:intron 35.0%
AAAAACAGGAACGAACCTAG+AGG - chr1.4:70917349-70917368 None:intergenic 40.0%
AAAACCATATTCCCGCAATC+AGG + chr1.4:70918204-70918223 MS.gene07162:intron 40.0%
AAAGTCGTAACCTGAAAGTG+GGG - chr1.4:70917107-70917126 None:intergenic 40.0%
AACTGCATTCACCAATCCAA+TGG + chr1.4:70918114-70918133 MS.gene07162:CDS 40.0%
AACTGTGCTTGAGCTGTATA+AGG - chr1.4:70919337-70919356 None:intergenic 40.0%
AAGATCACATGCATGCTTAG+AGG - chr1.4:70918032-70918051 None:intergenic 40.0%
AATGAATTCAGACATGGGGA+AGG + chr1.4:70918237-70918256 MS.gene07162:CDS 40.0%
ACAACTGACCTTGTTAAAGC+AGG - chr1.4:70917977-70917996 None:intergenic 40.0%
ACAAGGTCAGTTGTTTATGG+AGG + chr1.4:70917983-70918002 MS.gene07162:CDS 40.0%
ACTGAGATAGTCTAAAGGCA+GGG + chr1.4:70918906-70918925 MS.gene07162:intron 40.0%
AGATCACATGCATGCTTAGA+GGG - chr1.4:70918031-70918050 None:intergenic 40.0%
AGGCTACAAATGCAACTTGT+TGG + chr1.4:70917777-70917796 MS.gene07162:CDS 40.0%
AGGTAAGGTCTAAAGAGGAA+AGG - chr1.4:70917846-70917865 None:intergenic 40.0%
ATCCATGGGAGCAGTTATAA+GGG - chr1.4:70919376-70919395 None:intergenic 40.0%
ATGAATTCAGACATGGGGAA+GGG + chr1.4:70918238-70918257 MS.gene07162:CDS 40.0%
ATGTTGCAACGAGAATCCAA+GGG + chr1.4:70919414-70919433 MS.gene07162:CDS 40.0%
CAAAGTCGTAACCTGAAAGT+GGG - chr1.4:70917108-70917127 None:intergenic 40.0%
CAATCCAATGGAAGTTCTCA+AGG + chr1.4:70918126-70918145 MS.gene07162:CDS 40.0%
CAGGAGATGAGAAGCAAAAT+TGG + chr1.4:70917245-70917264 MS.gene07162:intron 40.0%
CCCTCAACTAATAGACCTAA+GGG - chr1.4:70918842-70918861 None:intergenic 40.0%
CCCTTAGGTCTATTAGTTGA+GGG + chr1.4:70918839-70918858 MS.gene07162:intron 40.0%
CCTCAACTAATAGACCTAAG+GGG - chr1.4:70918841-70918860 None:intergenic 40.0%
CTTGAGAACTTCCATTGGAT+TGG - chr1.4:70918128-70918147 None:intergenic 40.0%
GAAACAATGTCAAGAGGTGA+GGG + chr1.4:70918939-70918958 MS.gene07162:intron 40.0%
GTGATTGAAAACAGTGGAAG+GGG - chr1.4:70917278-70917297 None:intergenic 40.0%
GTTTGACACCTGCTTTAACA+AGG + chr1.4:70917966-70917985 MS.gene07162:CDS 40.0%
TAACAGTTGGAATTGTTGCC+AGG + chr1.4:70919081-70919100 MS.gene07162:intron 40.0%
TATAACTGCTCCCATGGATA+TGG + chr1.4:70919377-70919396 MS.gene07162:CDS 40.0%
TATCCATGGGAGCAGTTATA+AGG - chr1.4:70919377-70919396 None:intergenic 40.0%
TCACTTTCAAGACATCTGCA+CGG - chr1.4:70917758-70917777 None:intergenic 40.0%
TCCCTCAACTAATAGACCTA+AGG - chr1.4:70918843-70918862 None:intergenic 40.0%
TGAAACAATGTCAAGAGGTG+AGG + chr1.4:70918938-70918957 MS.gene07162:intron 40.0%
TGGATTTAACTGTGCATACC+AGG + chr1.4:70919455-70919474 MS.gene07162:CDS 40.0%
TTGGGACTGAGATAGTCTAA+AGG + chr1.4:70918901-70918920 MS.gene07162:intron 40.0%
! CACCCTTTATAAAGTGCTCT+TGG - chr1.4:70919796-70919815 None:intergenic 40.0%
! CATGCATGTGATCTTGCTTT+TGG + chr1.4:70918037-70918056 MS.gene07162:CDS 40.0%
! GAAGTTGTTGCCCATGATTT+TGG - chr1.4:70918639-70918658 None:intergenic 40.0%
! GGTGTGCTGATAGAAAACAT+AGG - chr1.4:70919222-70919241 None:intergenic 40.0%
! GTTTGGGATGCATTTGTACA+CGG + chr1.4:70919824-70919843 MS.gene07162:intron 40.0%
! TTTCAGGTCCTACTTACAGA+GGG + chr1.4:70919769-70919788 MS.gene07162:intron 40.0%
!! AATGCAACTTGTTGGTCAGA+AGG + chr1.4:70917785-70917804 MS.gene07162:CDS 40.0%
!! ATGCAACTTGTTGGTCAGAA+GGG + chr1.4:70917786-70917805 MS.gene07162:CDS 40.0%
!! GGTCCAAGAGCACTTTATAA+AGG + chr1.4:70919790-70919809 MS.gene07162:CDS 40.0%
!! TTAAGAGCATTTGTCGTGTG+TGG + chr1.4:70919742-70919761 MS.gene07162:intron 40.0%
!!! TTGCGGGAATATGGTTTTGA+AGG - chr1.4:70918202-70918221 None:intergenic 40.0%
AAGAGGGAATCAAAGCTCTG+TGG + chr1.4:70918296-70918315 MS.gene07162:CDS 45.0%
AATCAAAGCTCTGTGGAAGG+GGG + chr1.4:70918303-70918322 MS.gene07162:CDS 45.0%
AGGTAATCCGATGCTGACAA+AGG - chr1.4:70919202-70919221 None:intergenic 45.0%
ATCCGATGCTGACAAAGGAT+TGG - chr1.4:70919197-70919216 None:intergenic 45.0%
ATTGCTTCGGGAATGTTTGC+TGG + chr1.4:70918082-70918101 MS.gene07162:CDS 45.0%
CATGTTGCAACGAGAATCCA+AGG + chr1.4:70919413-70919432 MS.gene07162:CDS 45.0%
CCCCTTAGGTCTATTAGTTG+AGG + chr1.4:70918838-70918857 MS.gene07162:intron 45.0%
CCTCTTCAGATATAGTCCTC+CGG - chr1.4:70918282-70918301 None:intergenic 45.0%
CGGAGGACTATATCTGAAGA+GGG + chr1.4:70918280-70918299 MS.gene07162:CDS 45.0%
CTCACCTTGAGAACTTCCAT+TGG - chr1.4:70918133-70918152 None:intergenic 45.0%
GAAGGGTCCTTTAAGTGGAA+TGG + chr1.4:70917803-70917822 MS.gene07162:CDS 45.0%
GAATCAAAGCTCTGTGGAAG+GGG + chr1.4:70918302-70918321 MS.gene07162:CDS 45.0%
GACCAATCCTTTGTCAGCAT+CGG + chr1.4:70919192-70919211 MS.gene07162:intron 45.0%
GACTGAGATAGTCTAAAGGC+AGG + chr1.4:70918905-70918924 MS.gene07162:intron 45.0%
GAGGAGCTAAGGAAAATTGC+TGG + chr1.4:70919969-70919988 MS.gene07162:CDS 45.0%
GCAAAGTCGTAACCTGAAAG+TGG - chr1.4:70917109-70917128 None:intergenic 45.0%
GGAATCAAAGCTCTGTGGAA+GGG + chr1.4:70918301-70918320 MS.gene07162:CDS 45.0%
GTGCAGATGTCTTGAAAGTG+AGG + chr1.4:70917757-70917776 MS.gene07162:intron 45.0%
GTTTATGGAGGACTACGCTT+GGG + chr1.4:70917995-70918014 MS.gene07162:CDS 45.0%
TGTTTATGGAGGACTACGCT+TGG + chr1.4:70917994-70918013 MS.gene07162:CDS 45.0%
TTTCAACATTGGCACCCCTT+AGG + chr1.4:70918824-70918843 MS.gene07162:intron 45.0%
TTTGCAAACGCACCTGATTG+CGG - chr1.4:70918219-70918238 None:intergenic 45.0%
! ATCCGAGGTACGTTTTGTCA+GGG + chr1.4:70919890-70919909 MS.gene07162:intron 45.0%
! GATGCTGACAAAGGATTGGT+CGG - chr1.4:70919193-70919212 None:intergenic 45.0%
! GCTAAGGAAAATTGCTGGCT+TGG + chr1.4:70919974-70919993 MS.gene07162:CDS 45.0%
! GTTTCAGGTCCTACTTACAG+AGG + chr1.4:70919768-70919787 MS.gene07162:intron 45.0%
!! AAGGAAAATTGCTGGCTTGG+AGG + chr1.4:70919977-70919996 MS.gene07162:CDS 45.0%
!! CAAAGTACTGCAGGTTTGAG+AGG - chr1.4:70919499-70919518 None:intergenic 45.0%
!!! ATTAAATATAGATATTTTTA+AGG - chr1.4:70917722-70917741 None:intergenic 5.0%
AATTCAGACATGGGGAAGGG+CGG + chr1.4:70918241-70918260 MS.gene07162:CDS 50.0%
ACCTGAAAGTGGGGAATTCC+CGG - chr1.4:70917098-70917117 None:intergenic 50.0%
AGCCAACTGAGATGCAGTCA+AGG - chr1.4:70918356-70918375 None:intergenic 50.0%
CACCCTTATAACTGCTCCCA+TGG + chr1.4:70919371-70919390 MS.gene07162:CDS 50.0%
CACGGTTACATTGACCTACC+TGG - chr1.4:70918981-70919000 None:intergenic 50.0%
CCGGAGGACTATATCTGAAG+AGG + chr1.4:70918279-70918298 MS.gene07162:CDS 50.0%
CTCTTGGACCCTCTGTAAGT+AGG - chr1.4:70919780-70919799 None:intergenic 50.0%
GAAAACAGTGGAAGGGGACT+TGG - chr1.4:70917272-70917291 None:intergenic 50.0%
GAGCATTTGTCGTGTGTGGT+GGG + chr1.4:70919746-70919765 MS.gene07162:intron 50.0%
GGAATTGTTGCCAGGAGACT+AGG + chr1.4:70919089-70919108 MS.gene07162:intron 50.0%
GGGAATCAAAGCTCTGTGGA+AGG + chr1.4:70918300-70918319 MS.gene07162:CDS 50.0%
GGTCAGAAGGGTCCTTTAAG+TGG + chr1.4:70917798-70917817 MS.gene07162:CDS 50.0%
GTCAAGAGGTGAGGGAAATC+CGG + chr1.4:70918947-70918966 MS.gene07162:intron 50.0%
TACAGCTCAAGCACAGTTGC+AGG + chr1.4:70919339-70919358 MS.gene07162:intron 50.0%
TCAAGAGGTGAGGGAAATCC+GGG + chr1.4:70918948-70918967 MS.gene07162:intron 50.0%
TGCCTTGACTGCATCTCAGT+TGG + chr1.4:70918351-70918370 MS.gene07162:CDS 50.0%
TTGCAAACGCACCTGATTGC+GGG - chr1.4:70918218-70918237 None:intergenic 50.0%
TTGCAACGAGAATCCAAGGG+TGG + chr1.4:70919417-70919436 MS.gene07162:CDS 50.0%
! AGAGCATTTGTCGTGTGTGG+TGG + chr1.4:70919745-70919764 MS.gene07162:intron 50.0%
! CATCCGAGGTACGTTTTGTC+AGG + chr1.4:70919889-70919908 MS.gene07162:intron 50.0%
! TCCGAGGTACGTTTTGTCAG+GGG + chr1.4:70919891-70919910 MS.gene07162:intron 50.0%
!! GAGCAGTTATAAGGGTGCTC+AGG - chr1.4:70919368-70919387 None:intergenic 50.0%
!! GTCCTCCGGAGCTCTAAAAT+TGG - chr1.4:70918268-70918287 None:intergenic 50.0%
!!! GACCAATTTTAGAGCTCCGG+AGG + chr1.4:70918263-70918282 MS.gene07162:CDS 50.0%
!!! GCGGACCAATTTTAGAGCTC+CGG + chr1.4:70918260-70918279 MS.gene07162:CDS 50.0%
AAAGCTCTGTGGAAGGGGGT+GGG + chr1.4:70918307-70918326 MS.gene07162:CDS 55.0%
AGATCCGTGACCTAGTCTCC+TGG - chr1.4:70919102-70919121 None:intergenic 55.0%
CGCACCTGATTGCGGGAATA+TGG - chr1.4:70918211-70918230 None:intergenic 55.0%
CTGAGATGCAGTCAAGGCAG+CGG - chr1.4:70918350-70918369 None:intergenic 55.0%
GCCCCTGACAAAACGTACCT+CGG - chr1.4:70919895-70919914 None:intergenic 55.0%
GTGAGGGAAATCCGGGTCTT+GGG + chr1.4:70918955-70918974 MS.gene07162:intron 55.0%
GTTGCCAGGAGACTAGGTCA+CGG + chr1.4:70919095-70919114 MS.gene07162:intron 55.0%
!! TGTCGTGTGTGGTGGGTTTC+AGG + chr1.4:70919753-70919772 MS.gene07162:intron 55.0%
CAAAGCTCTGTGGAAGGGGG+TGG + chr1.4:70918306-70918325 MS.gene07162:CDS 60.0%
GCCGGGAATTCCCCACTTTC+AGG + chr1.4:70917094-70917113 MS.gene07162:CDS 60.0%
GGTGAGGGAAATCCGGGTCT+TGG + chr1.4:70918954-70918973 MS.gene07162:intron 60.0%
ATCCGGGTCTTGGGCCAGGT+AGG + chr1.4:70918964-70918983 MS.gene07162:intron 65.0%
GACCTACCTGGCCCAAGACC+CGG - chr1.4:70918969-70918988 None:intergenic 65.0%
GGAAATCCGGGTCTTGGGCC+AGG + chr1.4:70918960-70918979 MS.gene07162:intron 65.0%
TGCAGTCAAGGCAGCGGCTC+TGG - chr1.4:70918344-70918363 None:intergenic 65.0%
! CAAGGCAGCGGCTCTGGCCA+TGG - chr1.4:70918338-70918357 None:intergenic 70.0%
! CAGCGGCTCTGGCCATGGCA+GGG - chr1.4:70918333-70918352 None:intergenic 70.0%
GAAGGGGGTGGGCCCTGCCA+TGG + chr1.4:70918318-70918337 MS.gene07162:CDS 75.0%
! GCAGCGGCTCTGGCCATGGC+AGG - chr1.4:70918334-70918353 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 70917091 70920007 70917091 ID=MS.gene07162
chr1.4 mRNA 70917091 70920007 70917091 ID=MS.gene07162.t1;Parent=MS.gene07162
chr1.4 exon 70917091 70917115 70917091 ID=MS.gene07162.t1.exon1;Parent=MS.gene07162.t1
chr1.4 CDS 70917091 70917115 70917091 ID=cds.MS.gene07162.t1;Parent=MS.gene07162.t1
chr1.4 exon 70917248 70917352 70917248 ID=MS.gene07162.t1.exon2;Parent=MS.gene07162.t1
chr1.4 CDS 70917248 70917352 70917248 ID=cds.MS.gene07162.t1;Parent=MS.gene07162.t1
chr1.4 exon 70917763 70917824 70917763 ID=MS.gene07162.t1.exon3;Parent=MS.gene07162.t1
chr1.4 CDS 70917763 70917824 70917763 ID=cds.MS.gene07162.t1;Parent=MS.gene07162.t1
chr1.4 exon 70917911 70918147 70917911 ID=MS.gene07162.t1.exon4;Parent=MS.gene07162.t1
chr1.4 CDS 70917911 70918147 70917911 ID=cds.MS.gene07162.t1;Parent=MS.gene07162.t1
chr1.4 exon 70918226 70918396 70918226 ID=MS.gene07162.t1.exon5;Parent=MS.gene07162.t1
chr1.4 CDS 70918226 70918396 70918226 ID=cds.MS.gene07162.t1;Parent=MS.gene07162.t1
chr1.4 exon 70918500 70918549 70918500 ID=MS.gene07162.t1.exon6;Parent=MS.gene07162.t1
chr1.4 CDS 70918500 70918549 70918500 ID=cds.MS.gene07162.t1;Parent=MS.gene07162.t1
chr1.4 exon 70919344 70919476 70919344 ID=MS.gene07162.t1.exon7;Parent=MS.gene07162.t1
chr1.4 CDS 70919344 70919476 70919344 ID=cds.MS.gene07162.t1;Parent=MS.gene07162.t1
chr1.4 exon 70919775 70919812 70919775 ID=MS.gene07162.t1.exon8;Parent=MS.gene07162.t1
chr1.4 CDS 70919775 70919812 70919775 ID=cds.MS.gene07162.t1;Parent=MS.gene07162.t1
chr1.4 exon 70919911 70920007 70919911 ID=MS.gene07162.t1.exon9;Parent=MS.gene07162.t1
chr1.4 CDS 70919911 70920007 70919911 ID=cds.MS.gene07162.t1;Parent=MS.gene07162.t1
Gene Sequence

>MS.gene07162

ATGGCCGGGAATTCCCCACTTTCAGGTTACGACTTTGCTCCTTTTATTTCTTTGACCCATTTCTCTCTAACATAACATGTTAACAATGCATTATCACCATCACAAGTTGTAATTGTAATATTATATTGATGAAGATGAAAAATAATGAATGTATCAGGAGATGAGAAGCAAAATTGGGCCAAGTCCCCTTCCACTGTTTTCAATCACTTTGCAACAAGTGGACTTTCTGTCGCAGTTGCAACATCAATTACTCATCCTCTAGGTTCGTTCCTGTTTTTCTCTTTATAATAATAATATGAACGTGATATCAAATGACCAATTTGATTGATATCTTGTTGTATATTATATAGTTAGGGATAGATTTTGAGACTCAATTCAATACTACTACTACTCATTAGTGATGGCAAATGAATTCTGTGATGATTTATTGATACATCGAGGATAGATCTTTTAGGTAGTTATTTTATTCTTATTCTTTCTGGGTAGTCACACTTCACATGTCAAGTCAGCAGCTGTAGTAATTAAATAAAGATGTTATCATTAAGTTGGTGAAGTTCACATTGAGACCTTTAATAATATGTTGAGAAGAAACTATTTTATTTGGGTTTTGCAAAAGTAAATGACAAATCCTTAAAAATATCTATATTTAATTTGTTTCTACATTCCGTGCAGATGTCTTGAAAGTGAGGCTACAAATGCAACTTGTTGGTCAGAAGGGTCCTTTAAGTGGAATGGTAGTTCACTGAGTTCAACCTTTCCTCTTTAGACCTTACCTATTACATTATAATACAAAACTTTTGTCAATTAAGCATTTTTCCAGGGACAATTGTTTTTAAGTGTGGTGAAAAATGAAGGACCTAAGTCTTTATATCTGGGTTTGACACCTGCTTTAACAAGGTCAGTTGTTTATGGAGGACTACGCTTGGGTTTGTACGAACCCTCTAAGCATGCATGTGATCTTGCTTTTGGTTCTTCCAATGTTTTAGTTAAAATTGCTTCGGGAATGTTTGCTGGTGCTTTTGCAACTGCATTCACCAATCCAATGGAAGTTCTCAAGGTGAGCTATCATATCTTTTACCTGTACATTAAGCAAAAAAAAATTGTTTCTCCTTCAAAACCATATTCCCGCAATCAGGTGCGTTTGCAAATGAATTCAGACATGGGGAAGGGCGGACCAATTTTAGAGCTCCGGAGGACTATATCTGAAGAGGGAATCAAAGCTCTGTGGAAGGGGGTGGGCCCTGCCATGGCCAGAGCCGCTGCCTTGACTGCATCTCAGTTGGCTACTTATGATGAAACCAAGCAGGTCAATTTCTAAAGTGTATTTTTGTTCATCTATTCCTCATCTCCCTAATTTACTTTCAATTAAATCATTGTTTTTCTAAATTTGTTTTGCTTTGCTTATGCAGATTCTAGTCAAATGGACATCTCTCAAAGAAGGATTTCATCTACATCTTATGTATGGCTCTCTCGTCATTTTTATTTCTCCATATCTCTCTTAATGCAGACTGAACTAATGAAGCATCATTAGAGCCCAAAGATTGACCAAAATCATGGGCAACAACTTCACCATATAGTGAAGAATAATGAAGATTGACATATCTTGAAAAATGAAATTCGCATAACTAATGTTTGTTTTATAATTGTTTTTGCGTGCATAAAATATATTCAAACTGAAGTGCATATTTTCTTACAAAACTAGTTTTGTTTGTCTAATTAATTGAAACAATGATTTTCAACATTGGCACCCCTTAGGTCTATTAGTTGAGGGAGAGAAAGTTAGATAGTTAGTTTGACATTAGTTGGGAACTTGGGACTGAGATAGTCTAAAGGCAGGGATTGTTATATGAAACAATGTCAAGAGGTGAGGGAAATCCGGGTCTTGGGCCAGGTAGGTCAATGTAACCGTGAAGAATTTGTGTTCTTATCTGTAGTCAGCGAAATTCTTCCTTGTGTTTTCTAATAAAATACAATAAGGGAAACCTAATTGTAACAGTTGGAATTGTTGCCAGGAGACTAGGTCACGGATCTAAGTTGTGACATTAACTTGTTGCAAATGCAAGATTAGGCAGCCAGCCTGCAATGAATTCTAAATGGATCCGACCAATCCTTTGTCAGCATCGGATTACCTATGTTTTCTATCAGCACACCACACAAGTCTGAATTCATTAGAATTATTACTATTTTGAAATCAAAACCATTTTCCATTATCAGAGATGTGACTTAACTGATTGTGTTATGTACCTTATACAGCTCAAGCACAGTTGCAGGCATCCTGAGCACCCTTATAACTGCTCCCATGGATATGGTTAAAACACGTCTCATGTTGCAACGAGAATCCAAGGGTGGTAGAATATATAAAAATGGATTTAACTGTGCATACCAGGTATTAAATTGTTTTCTCACCTCTCAAACCTGCAGTACTTTGTTTGTTCTATACTGATCTCTGCTTCTTTATTTTAAGTGGTTGACTTTAGTTCATTACTTTGTACTGTGCCATATAAGTTCTATACTTCTTTCATACATTCTTTAACTTTGACTAGGCTTTCCTAATTCTTAGTTATTAGCTTTTAATGGAGTGATAACGAAATGACTTTCGTAAAACCCAATCATGTAAAACAATGATATATTTTCTGATCTGGATTCACTTATTAAGAGCATTTGTCGTGTGTGGTGGGTTTCAGGTCCTACTTACAGAGGGTCCAAGAGCACTTTATAAAGGGTGAGTTTTATGTTTGGGATGCATTTGTACACGGAGCTTGTTTGTTTAATTCATCCTAAACTTTAATGCTATTTAACATCCGAGGTACGTTTTGTCAGGGGCTTCACACTATTTGCAAGATTAGGACCACAAACCACAATTACATTTATATTATGTGAGGAGCTAAGGAAAATTGCTGGCTTGGAGGCAATTTAG
Protein sequence

>MS.gene07162.t1

MAGNSPLSGDEKQNWAKSPSTVFNHFATSGLSVAVATSITHPLDVLKVRLQMQLVGQKGPLSGMGQLFLSVVKNEGPKSLYLGLTPALTRSVVYGGLRLGLYEPSKHACDLAFGSSNVLVKIASGMFAGAFATAFTNPMEVLKVRLQMNSDMGKGGPILELRRTISEEGIKALWKGVGPAMARAAALTASQLATYDETKQILVKWTSLKEGFHLHLISSTVAGILSTLITAPMDMVKTRLMLQRESKGGRIYKNGFNCAYQVLLTEGPRALYKGGFTLFARLGPQTTITFILCEELRKIAGLEAI