Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071679.t1 | KEH27297.1 | 99.1 | 226 | 2 | 0 | 1 | 226 | 1 | 226 | 3.20E-120 | 441 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071679.t1 | Q8GYX8 | 86.7 | 226 | 29 | 1 | 1 | 226 | 1 | 225 | 1.7e-106 | 386.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071679.t1 | A0A072UCG3 | 99.1 | 226 | 2 | 0 | 1 | 226 | 1 | 226 | 2.3e-120 | 441.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049711 | MS.gene071679 | 0.801839 | 7.45E-49 | -1.69E-46 |
| MS.gene049744 | MS.gene071679 | 0.840585 | 7.96E-58 | -1.69E-46 |
| MS.gene050257 | MS.gene071679 | 0.840696 | 7.44E-58 | -1.69E-46 |
| MS.gene050258 | MS.gene071679 | 0.829041 | 6.42E-55 | -1.69E-46 |
| MS.gene050348 | MS.gene071679 | 0.829235 | 5.77E-55 | -1.69E-46 |
| MS.gene050646 | MS.gene071679 | 0.826349 | 2.85E-54 | -1.69E-46 |
| MS.gene051209 | MS.gene071679 | 0.809393 | 1.94E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene071679 | 0.801289 | 9.66E-49 | -1.69E-46 |
| MS.gene052037 | MS.gene071679 | 0.810757 | 9.84E-51 | -1.69E-46 |
| MS.gene052307 | MS.gene071679 | 0.83306 | 6.62E-56 | -1.69E-46 |
| MS.gene052514 | MS.gene071679 | 0.803556 | 3.29E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene071679 | 0.859951 | 2.90E-63 | -1.69E-46 |
| MS.gene052775 | MS.gene071679 | 0.802041 | 6.77E-49 | -1.69E-46 |
| MS.gene052776 | MS.gene071679 | 0.811724 | 6.07E-51 | -1.69E-46 |
| MS.gene053143 | MS.gene071679 | 0.804909 | 1.72E-49 | -1.69E-46 |
| MS.gene053620 | MS.gene071679 | 0.83623 | 1.06E-56 | -1.69E-46 |
| MS.gene054892 | MS.gene071679 | 0.805845 | 1.10E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene071679 | 0.805288 | 1.44E-49 | -1.69E-46 |
| MS.gene056507 | MS.gene071679 | 0.839965 | 1.16E-57 | -1.69E-46 |
| MS.gene056519 | MS.gene071679 | 0.867044 | 1.83E-65 | -1.69E-46 |
| MS.gene05686 | MS.gene071679 | 0.846684 | 1.86E-59 | -1.69E-46 |
| MS.gene056973 | MS.gene071679 | 0.828381 | 9.28E-55 | -1.69E-46 |
| MS.gene057174 | MS.gene071679 | 0.87695 | 9.29E-69 | -1.69E-46 |
| MS.gene058289 | MS.gene071679 | 0.846358 | 2.28E-59 | -1.69E-46 |
| MS.gene059634 | MS.gene071679 | 0.814399 | 1.57E-51 | -1.69E-46 |
| MS.gene060522 | MS.gene071679 | 0.806301 | 8.79E-50 | -1.69E-46 |
| MS.gene060785 | MS.gene071679 | 0.841239 | 5.36E-58 | -1.69E-46 |
| MS.gene060933 | MS.gene071679 | 0.857971 | 1.13E-62 | -1.69E-46 |
| MS.gene060937 | MS.gene071679 | 0.812367 | 4.39E-51 | -1.69E-46 |
| MS.gene061188 | MS.gene071679 | 0.811362 | 7.27E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071679.t1 | MTR_5g004570 | 99.115 | 226 | 2 | 0 | 1 | 226 | 1 | 226 | 4.25e-169 | 465 |
| MS.gene071679.t1 | MTR_1g029470 | 64.000 | 225 | 76 | 1 | 1 | 225 | 1 | 220 | 1.32e-104 | 307 |
| MS.gene071679.t1 | MTR_8g037810 | 61.404 | 228 | 86 | 1 | 1 | 226 | 1 | 228 | 1.15e-99 | 293 |
| MS.gene071679.t1 | MTR_1g029460 | 57.333 | 225 | 91 | 1 | 1 | 225 | 1 | 220 | 2.63e-88 | 266 |
| MS.gene071679.t1 | MTR_3g027580 | 53.623 | 69 | 32 | 0 | 6 | 74 | 89 | 157 | 6.30e-17 | 79.3 |
| MS.gene071679.t1 | MTR_4g023470 | 57.576 | 66 | 28 | 0 | 6 | 71 | 86 | 151 | 6.70e-16 | 76.3 |
| MS.gene071679.t1 | MTR_4g080350 | 38.793 | 116 | 67 | 3 | 6 | 120 | 72 | 184 | 1.31e-15 | 74.7 |
| MS.gene071679.t1 | MTR_2g083920 | 38.793 | 116 | 67 | 3 | 6 | 120 | 70 | 182 | 2.30e-15 | 73.6 |
| MS.gene071679.t1 | MTR_2g083920 | 38.793 | 116 | 67 | 3 | 6 | 120 | 70 | 182 | 6.79e-15 | 72.8 |
| MS.gene071679.t1 | MTR_8g006430 | 50.704 | 71 | 31 | 2 | 5 | 75 | 13 | 79 | 2.02e-14 | 72.0 |
| MS.gene071679.t1 | MTR_5g012530 | 45.977 | 87 | 38 | 3 | 6 | 84 | 12 | 97 | 2.58e-14 | 71.6 |
| MS.gene071679.t1 | MTR_2g104030 | 38.889 | 108 | 62 | 3 | 5 | 112 | 69 | 172 | 8.76e-14 | 70.1 |
| MS.gene071679.t1 | MTR_5g035770 | 45.833 | 72 | 39 | 0 | 8 | 79 | 27 | 98 | 1.11e-13 | 69.7 |
| MS.gene071679.t1 | MTR_1g110310 | 40.860 | 93 | 46 | 2 | 8 | 91 | 18 | 110 | 1.64e-13 | 69.3 |
| MS.gene071679.t1 | MTR_3g087030 | 47.222 | 72 | 37 | 1 | 4 | 75 | 80 | 150 | 1.73e-13 | 69.3 |
| MS.gene071679.t1 | MTR_7g109340 | 39.785 | 93 | 49 | 1 | 7 | 92 | 27 | 119 | 3.91e-13 | 67.8 |
| MS.gene071679.t1 | MTR_5g065160 | 33.577 | 137 | 76 | 3 | 4 | 125 | 81 | 217 | 4.38e-13 | 68.2 |
| MS.gene071679.t1 | MTR_3g108020 | 40.000 | 100 | 48 | 3 | 6 | 93 | 4 | 103 | 4.58e-13 | 67.4 |
| MS.gene071679.t1 | MTR_5g065160 | 33.577 | 137 | 76 | 3 | 4 | 125 | 80 | 216 | 4.59e-13 | 67.8 |
| MS.gene071679.t1 | MTR_5g065160 | 33.577 | 137 | 76 | 3 | 4 | 125 | 81 | 217 | 5.76e-13 | 67.8 |
| MS.gene071679.t1 | MTR_5g065160 | 33.577 | 137 | 76 | 3 | 4 | 125 | 74 | 210 | 5.88e-13 | 67.8 |
| MS.gene071679.t1 | MTR_2g014480 | 45.070 | 71 | 38 | 1 | 6 | 75 | 4 | 74 | 6.36e-13 | 67.4 |
| MS.gene071679.t1 | MTR_4g015840 | 45.070 | 71 | 38 | 1 | 6 | 75 | 4 | 74 | 1.71e-12 | 65.9 |
| MS.gene071679.t1 | MTR_7g092380 | 50.000 | 64 | 32 | 0 | 8 | 71 | 24 | 87 | 2.62e-12 | 65.1 |
| MS.gene071679.t1 | MTR_2g014470 | 45.070 | 71 | 38 | 1 | 6 | 75 | 4 | 74 | 4.28e-12 | 64.7 |
| MS.gene071679.t1 | MTR_1g105540 | 47.692 | 65 | 33 | 1 | 4 | 68 | 33 | 96 | 1.56e-11 | 63.2 |
| MS.gene071679.t1 | MTR_4g023490 | 51.515 | 66 | 31 | 1 | 6 | 71 | 16 | 80 | 2.46e-11 | 62.8 |
| MS.gene071679.t1 | MTR_1g052110 | 40.230 | 87 | 49 | 1 | 8 | 91 | 40 | 126 | 3.24e-11 | 62.0 |
| MS.gene071679.t1 | MTR_4g078850 | 43.662 | 71 | 39 | 1 | 6 | 75 | 4 | 74 | 5.58e-11 | 61.6 |
| MS.gene071679.t1 | MTR_2g090705 | 34.821 | 112 | 72 | 1 | 6 | 116 | 4 | 115 | 6.50e-11 | 61.2 |
| MS.gene071679.t1 | MTR_5g065860 | 39.024 | 82 | 50 | 0 | 1 | 82 | 43 | 124 | 6.55e-11 | 58.9 |
| MS.gene071679.t1 | MTR_7g112360 | 46.269 | 67 | 32 | 1 | 7 | 69 | 17 | 83 | 9.80e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071679.t1 | AT1G76700 | 86.726 | 226 | 29 | 1 | 1 | 226 | 1 | 225 | 6.83e-144 | 407 |
| MS.gene071679.t1 | AT1G21080 | 83.186 | 226 | 36 | 2 | 1 | 226 | 1 | 224 | 1.60e-136 | 389 |
| MS.gene071679.t1 | AT1G21080 | 83.186 | 226 | 36 | 2 | 1 | 226 | 1 | 224 | 1.84e-136 | 389 |
| MS.gene071679.t1 | AT1G77020 | 65.778 | 225 | 75 | 1 | 1 | 225 | 1 | 223 | 1.17e-106 | 312 |
| MS.gene071679.t1 | AT4G39150 | 60.965 | 228 | 87 | 1 | 1 | 226 | 1 | 228 | 1.56e-99 | 293 |
| MS.gene071679.t1 | AT4G39150 | 60.965 | 228 | 87 | 1 | 1 | 226 | 1 | 228 | 1.56e-99 | 293 |
| MS.gene071679.t1 | AT4G39150 | 60.965 | 228 | 87 | 1 | 1 | 226 | 1 | 228 | 1.56e-99 | 293 |
| MS.gene071679.t1 | AT2G21510 | 57.895 | 228 | 94 | 1 | 1 | 226 | 1 | 228 | 4.15e-96 | 283 |
| MS.gene071679.t1 | AT2G21510 | 57.895 | 228 | 94 | 1 | 1 | 226 | 1 | 228 | 4.50e-96 | 284 |
| MS.gene071679.t1 | AT2G21510 | 57.895 | 228 | 94 | 1 | 1 | 226 | 1 | 228 | 4.50e-96 | 284 |
| MS.gene071679.t1 | AT2G21510 | 57.895 | 228 | 94 | 1 | 1 | 226 | 1 | 228 | 4.82e-96 | 284 |
| MS.gene071679.t1 | AT2G21510 | 57.895 | 228 | 94 | 1 | 1 | 226 | 1 | 228 | 4.82e-96 | 284 |
| MS.gene071679.t1 | AT1G21080 | 80.435 | 138 | 26 | 1 | 89 | 226 | 1 | 137 | 4.15e-76 | 232 |
| MS.gene071679.t1 | AT1G59980 | 41.935 | 93 | 45 | 2 | 8 | 91 | 25 | 117 | 2.92e-15 | 74.3 |
| MS.gene071679.t1 | AT1G59980 | 41.935 | 93 | 45 | 2 | 8 | 91 | 25 | 117 | 3.20e-15 | 73.9 |
| MS.gene071679.t1 | AT1G59725 | 46.575 | 73 | 37 | 1 | 6 | 76 | 4 | 76 | 1.58e-14 | 71.6 |
| MS.gene071679.t1 | AT2G42750 | 37.069 | 116 | 69 | 3 | 6 | 120 | 76 | 188 | 2.21e-14 | 71.6 |
| MS.gene071679.t1 | AT5G48030 | 51.515 | 66 | 32 | 0 | 6 | 71 | 94 | 159 | 2.33e-14 | 72.0 |
| MS.gene071679.t1 | AT5G25530 | 38.532 | 109 | 47 | 3 | 6 | 96 | 4 | 110 | 2.74e-14 | 71.2 |
| MS.gene071679.t1 | AT1G28210 | 50.000 | 62 | 31 | 0 | 7 | 68 | 49 | 110 | 4.59e-14 | 70.9 |
| MS.gene071679.t1 | AT1G28210 | 50.000 | 62 | 31 | 0 | 7 | 68 | 49 | 110 | 4.73e-14 | 70.9 |
| MS.gene071679.t1 | AT1G28210 | 50.000 | 62 | 31 | 0 | 7 | 68 | 49 | 110 | 5.12e-14 | 70.9 |
| MS.gene071679.t1 | AT1G10350 | 48.000 | 75 | 37 | 2 | 6 | 78 | 4 | 78 | 3.20e-13 | 68.2 |
| MS.gene071679.t1 | AT1G80030 | 36.154 | 130 | 72 | 4 | 6 | 135 | 75 | 193 | 5.54e-13 | 67.8 |
| MS.gene071679.t1 | AT1G80030 | 36.154 | 130 | 72 | 4 | 6 | 135 | 75 | 193 | 5.54e-13 | 67.8 |
| MS.gene071679.t1 | AT1G80030 | 36.154 | 130 | 72 | 4 | 6 | 135 | 75 | 193 | 5.54e-13 | 67.8 |
| MS.gene071679.t1 | AT1G80030 | 38.318 | 107 | 62 | 3 | 6 | 112 | 75 | 177 | 6.36e-13 | 67.8 |
| MS.gene071679.t1 | AT2G22360 | 40.426 | 94 | 49 | 2 | 4 | 91 | 84 | 176 | 7.52e-13 | 67.4 |
| MS.gene071679.t1 | AT4G28480 | 40.206 | 97 | 52 | 2 | 6 | 96 | 4 | 100 | 9.67e-13 | 66.2 |
| MS.gene071679.t1 | AT3G08910 | 45.333 | 75 | 40 | 1 | 6 | 79 | 4 | 78 | 9.78e-13 | 66.6 |
| MS.gene071679.t1 | AT4G28480 | 40.206 | 97 | 52 | 2 | 6 | 96 | 4 | 100 | 1.08e-12 | 66.6 |
| MS.gene071679.t1 | AT2G20560 | 46.479 | 71 | 37 | 1 | 6 | 75 | 4 | 74 | 1.48e-12 | 66.2 |
| MS.gene071679.t1 | AT5G01390 | 44.444 | 72 | 39 | 1 | 6 | 76 | 4 | 75 | 1.73e-12 | 65.1 |
| MS.gene071679.t1 | AT5G25530 | 36.752 | 117 | 46 | 4 | 6 | 96 | 4 | 118 | 3.23e-12 | 65.1 |
| MS.gene071679.t1 | AT5G01390 | 44.444 | 72 | 39 | 1 | 6 | 76 | 4 | 75 | 3.87e-12 | 63.9 |
| MS.gene071679.t1 | AT2G35720 | 43.210 | 81 | 43 | 2 | 6 | 83 | 15 | 95 | 4.01e-12 | 65.5 |
| MS.gene071679.t1 | AT3G47940 | 44.156 | 77 | 41 | 1 | 6 | 80 | 4 | 80 | 4.11e-12 | 65.1 |
| MS.gene071679.t1 | AT5G01390 | 44.444 | 72 | 39 | 1 | 6 | 76 | 4 | 75 | 5.81e-12 | 63.9 |
| MS.gene071679.t1 | AT1G68370 | 39.785 | 93 | 47 | 2 | 8 | 91 | 19 | 111 | 6.53e-12 | 64.7 |
| MS.gene071679.t1 | AT5G01390 | 44.444 | 72 | 39 | 1 | 6 | 76 | 4 | 75 | 9.53e-12 | 63.9 |
| MS.gene071679.t1 | AT4G21180 | 45.333 | 75 | 36 | 3 | 1 | 71 | 91 | 164 | 1.04e-11 | 64.3 |
| MS.gene071679.t1 | AT4G21180 | 45.333 | 75 | 36 | 3 | 1 | 71 | 11 | 84 | 1.14e-11 | 63.9 |
| MS.gene071679.t1 | AT1G24120 | 45.312 | 64 | 35 | 0 | 8 | 71 | 22 | 85 | 1.25e-11 | 63.9 |
| MS.gene071679.t1 | AT3G12170 | 37.363 | 91 | 54 | 1 | 4 | 91 | 45 | 135 | 2.17e-11 | 62.4 |
| MS.gene071679.t1 | AT4G39960 | 43.056 | 72 | 40 | 1 | 4 | 75 | 83 | 153 | 2.19e-11 | 63.2 |
| MS.gene071679.t1 | AT4G39960 | 43.056 | 72 | 40 | 1 | 4 | 75 | 83 | 153 | 2.19e-11 | 63.2 |
| MS.gene071679.t1 | AT3G12170 | 37.363 | 91 | 54 | 1 | 4 | 91 | 9 | 99 | 2.40e-11 | 62.0 |
| MS.gene071679.t1 | AT3G62600 | 35.294 | 102 | 56 | 1 | 7 | 98 | 27 | 128 | 3.76e-11 | 62.0 |
Find 50 sgRNAs with CRISPR-Local
Find 146 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGTTTCCCTGGACATAC+TGG | 0.285874 | 5.3:-130265 | None:intergenic |
| TATGATGCACATGGCAAATC+TGG | 0.306958 | 5.3:+129098 | MS.gene071679:CDS |
| CACCTGTTGCCGCTGTAACT+TGG | 0.313148 | 5.3:-130797 | None:intergenic |
| GGGCAGCAAGAGGGTCATTT+GGG | 0.335034 | 5.3:-128901 | None:intergenic |
| CTAAACCACTCAGCAATAAA+AGG | 0.342495 | 5.3:-130750 | None:intergenic |
| TGGGCAGCAAGAGGGTCATT+TGG | 0.353660 | 5.3:-128902 | None:intergenic |
| CCATTGATGCCATGGCTAAC+TGG | 0.362537 | 5.3:-129289 | None:intergenic |
| AGGCTTGTAGAGATACTAAA+AGG | 0.364783 | 5.3:+130233 | MS.gene071679:CDS |
| AGCTTTCTTAATCTCAGATT+CGG | 0.380678 | 5.3:-128717 | None:intergenic |
| ACCTGTTGCCGCTGTAACTT+GGG | 0.383531 | 5.3:-130796 | None:intergenic |
| GTTGTCATTATACAGCTTAT+GGG | 0.384762 | 5.3:+130643 | MS.gene071679:intron |
| CATAAGCTTGCCTTTGAGTT+GGG | 0.400101 | 5.3:-129079 | None:intergenic |
| CAAAGTCTTCTTTGTTTCCC+TGG | 0.407211 | 5.3:-130274 | None:intergenic |
| TCATAAGCTTGCCTTTGAGT+TGG | 0.415070 | 5.3:-129080 | None:intergenic |
| TCGTACCTGCATTGGACAGC+CGG | 0.441407 | 5.3:-130322 | None:intergenic |
| TCCCAAGTTACAGCGGCAAC+AGG | 0.444241 | 5.3:+130795 | MS.gene071679:CDS |
| CCAGTTAGCCATGGCATCAA+TGG | 0.450422 | 5.3:+129289 | MS.gene071679:CDS |
| ACAGGCAAGGCAAGTGCATC+CGG | 0.467108 | 5.3:+128871 | MS.gene071679:intron |
| CATGGCATCAATGGCTTCCA+TGG | 0.469034 | 5.3:+129298 | MS.gene071679:CDS |
| GATTGCAGCAGGATCAATAA+TGG | 0.492454 | 5.3:-129222 | None:intergenic |
| TAAGAAAGCTTATTACATCA+AGG | 0.495025 | 5.3:+128730 | MS.gene071679:CDS |
| ATCTCAGATTCGGATGCGGT+TGG | 0.495393 | 5.3:-128707 | None:intergenic |
| GAGTTGGGTCACTGAGAACT+TGG | 0.499446 | 5.3:-129064 | None:intergenic |
| AGGCAGGCAGCAAAAGAGCT+AGG | 0.503290 | 5.3:+130702 | MS.gene071679:CDS |
| TTAGGGCATCGTACCTGCAT+TGG | 0.506072 | 5.3:-130330 | None:intergenic |
| CTTCCGCCAGGTTGTTCAGA+AGG | 0.520281 | 5.3:+130199 | MS.gene071679:intron |
| CTGAGTGGTTTAGAAACAAA+GGG | 0.533228 | 5.3:+130760 | MS.gene071679:CDS |
| CAGACTCAACCAGTATGTCC+AGG | 0.533241 | 5.3:+130256 | MS.gene071679:CDS |
| TTCAGAAGGAGAGAGAAGAA+AGG | 0.535912 | 5.3:+130213 | MS.gene071679:CDS |
| CTTAATCTCAGATTCGGATG+CGG | 0.535962 | 5.3:-128711 | None:intergenic |
| GTTGATATGTTGAATACCAT+TGG | 0.536608 | 5.3:+130666 | MS.gene071679:CDS |
| AGCATGGCAAAGATTGCAGC+AGG | 0.536765 | 5.3:-129233 | None:intergenic |
| ATGCAGAAGCTGAAGTAGCC+CGG | 0.542752 | 5.3:+130303 | MS.gene071679:CDS |
| CGTACCTGCATTGGACAGCC+GGG | 0.553498 | 5.3:-130321 | None:intergenic |
| AGCGAATACTATGACGTGCT+TGG | 0.565955 | 5.3:+128677 | MS.gene071679:CDS |
| GTAGCCCGGCTGTCCAATGC+AGG | 0.566818 | 5.3:+130317 | MS.gene071679:CDS |
| TTGTCATTATACAGCTTATG+GGG | 0.568921 | 5.3:+130644 | MS.gene071679:intron |
| TGGCTACATATACGCAAGGC+AGG | 0.571362 | 5.3:+130686 | MS.gene071679:CDS |
| GCTGAGTGGTTTAGAAACAA+AGG | 0.575329 | 5.3:+130759 | MS.gene071679:CDS |
| TTCTCAGTGACCCAACTCAA+AGG | 0.583766 | 5.3:+129069 | MS.gene071679:CDS |
| GGAAAGATCAATCATGGTGA+AGG | 0.584644 | 5.3:+128652 | None:intergenic |
| GGCAGGCAGCAAAAGAGCTA+GGG | 0.591181 | 5.3:+130703 | MS.gene071679:CDS |
| AAAATTGCAAGACAAAATGC+GGG | 0.593137 | 5.3:+129355 | MS.gene071679:CDS |
| ATATATAGGCCAGTTAGCCA+TGG | 0.607750 | 5.3:+129280 | MS.gene071679:CDS |
| CATGGAAGCCATTGATGCCA+TGG | 0.610809 | 5.3:-129297 | None:intergenic |
| AGGCAAGCTTATGATGCACA+TGG | 0.629597 | 5.3:+129089 | MS.gene071679:CDS |
| AGACTCAACCAGTATGTCCA+GGG | 0.639373 | 5.3:+130257 | MS.gene071679:CDS |
| CCTTGCGTATATGTAGCCAA+TGG | 0.645015 | 5.3:-130682 | None:intergenic |
| CCATTGGCTACATATACGCA+AGG | 0.657826 | 5.3:+130682 | MS.gene071679:CDS |
| CATCAAATCCCAAGTTACAG+CGG | 0.686887 | 5.3:+130788 | MS.gene071679:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTCATTAAATTTATCAAT+AGG | + | chr5.3:129543-129562 | MS.gene071679:intron | 10.0% |
| !! | TAATTAATTAAAAAAAAGAG+AGG | - | chr5.3:129156-129175 | None:intergenic | 10.0% |
| !! | TTTCATTAAATTTATCAATA+GGG | + | chr5.3:129544-129563 | MS.gene071679:intron | 10.0% |
| !! | TGTTCAATTATTTATTTAAC+AGG | + | chr5.3:128853-128872 | MS.gene071679:intron | 15.0% |
| !! | TTCATTAAATTTATCAATAG+GGG | + | chr5.3:129545-129564 | MS.gene071679:intron | 15.0% |
| !!! | ATTCTTGTAATGTAATTTTA+CGG | - | chr5.3:130401-130420 | None:intergenic | 15.0% |
| !! | AAAAGTAAAAACTAACAGAT+CGG | - | chr5.3:129436-129455 | None:intergenic | 20.0% |
| !! | AATAAATGTGAAAAAGGTAA+AGG | + | chr5.3:129815-129834 | MS.gene071679:intron | 20.0% |
| !! | AATTATTTATTTAACAGGCA+AGG | + | chr5.3:128858-128877 | MS.gene071679:intron | 20.0% |
| !! | ATAAATGTGAAAAAGGTAAA+GGG | + | chr5.3:129816-129835 | MS.gene071679:intron | 20.0% |
| !! | CATGTTTAAAAAATCATGAA+TGG | + | chr5.3:130517-130536 | MS.gene071679:intron | 20.0% |
| !! | GTTTATATTTATTCTGTCTT+AGG | + | chr5.3:130138-130157 | MS.gene071679:intron | 20.0% |
| !! | TTAATTAAAAAAAAGAGAGG+AGG | - | chr5.3:129153-129172 | None:intergenic | 20.0% |
| !! | TTTCGTAATAAATGTGAAAA+AGG | + | chr5.3:129809-129828 | MS.gene071679:intron | 20.0% |
| !!! | GTTAGTTTTTACTTTTGTAA+TGG | + | chr5.3:129440-129459 | MS.gene071679:intron | 20.0% |
| ! | AAAAATTGCAAGACAAAATG+CGG | + | chr5.3:129354-129373 | MS.gene071679:CDS | 25.0% |
| ! | AAAATAATTTCTGACATGCA+TGG | + | chr5.3:130351-130370 | MS.gene071679:intron | 25.0% |
| ! | AAATACTTGAGAACATGTAA+AGG | - | chr5.3:130576-130595 | None:intergenic | 25.0% |
| ! | AGTTAACTCTTACGATAATA+AGG | + | chr5.3:130600-130619 | MS.gene071679:intron | 25.0% |
| ! | ATCATCAATATAACACAAAC+TGG | - | chr5.3:128813-128832 | None:intergenic | 25.0% |
| ! | GCATCTAGTAATTTGAATAA+GGG | - | chr5.3:129639-129658 | None:intergenic | 25.0% |
| ! | TAAAAAAGAAAGAAAGAAGC+AGG | - | chr5.3:128763-128782 | None:intergenic | 25.0% |
| ! | TAAGAAAGCTTATTACATCA+AGG | + | chr5.3:128730-128749 | MS.gene071679:CDS | 25.0% |
| ! | TGCATCTAGTAATTTGAATA+AGG | - | chr5.3:129640-129659 | None:intergenic | 25.0% |
| ! | TTACAAGAATGCAATGTTAA+TGG | + | chr5.3:130411-130430 | MS.gene071679:intron | 25.0% |
| ! | TTCATGCTTTGATACATATT+TGG | + | chr5.3:129955-129974 | MS.gene071679:intron | 25.0% |
| !! | TATTAATTAGGGGTATGTTT+AGG | - | chr5.3:129677-129696 | None:intergenic | 25.0% |
| !! | TGCATGTCAGAAATTATTTT+AGG | - | chr5.3:130351-130370 | None:intergenic | 25.0% |
| !! | TTTTCCTTCAAATTTCTTGT+TGG | - | chr5.3:129474-129493 | None:intergenic | 25.0% |
| !!! | AAGTTGGTAGTATTAATTAG+GGG | - | chr5.3:129687-129706 | None:intergenic | 25.0% |
| !!! | ACAAGTTGGTAGTATTAATT+AGG | - | chr5.3:129689-129708 | None:intergenic | 25.0% |
| !!! | AGATTTTGACCTGAAAATTT+TGG | - | chr5.3:128925-128944 | None:intergenic | 25.0% |
| !!! | AGATTTTTTGTAGTATAAGC+AGG | + | chr5.3:129879-129898 | MS.gene071679:intron | 25.0% |
| !!! | CAAGTTGGTAGTATTAATTA+GGG | - | chr5.3:129688-129707 | None:intergenic | 25.0% |
| !!! | GATTTTGACCTGAAAATTTT+GGG | - | chr5.3:128924-128943 | None:intergenic | 25.0% |
| !!! | GATTTTTTGTAGTATAAGCA+GGG | + | chr5.3:129880-129899 | MS.gene071679:intron | 25.0% |
| !!! | GCATGTCAGAAATTATTTTA+GGG | - | chr5.3:130350-130369 | None:intergenic | 25.0% |
| AAAATTGCAAGACAAAATGC+GGG | + | chr5.3:129355-129374 | MS.gene071679:CDS | 30.0% | |
| AATAAATTTATTCTTCCGCC+AGG | + | chr5.3:130187-130206 | MS.gene071679:intron | 30.0% | |
| AGAGAAAGAAATCATGAACA+TGG | - | chr5.3:129989-130008 | None:intergenic | 30.0% | |
| AGATTGTCTTTACATGACAT+TGG | + | chr5.3:130545-130564 | MS.gene071679:intron | 30.0% | |
| AGCTTTCTTAATCTCAGATT+CGG | - | chr5.3:128720-128739 | None:intergenic | 30.0% | |
| AGTTGTCATTATACAGCTTA+TGG | + | chr5.3:130642-130661 | MS.gene071679:intron | 30.0% | |
| ATCAATATAACACAAACTGG+TGG | - | chr5.3:128810-128829 | None:intergenic | 30.0% | |
| CTAGTAATTTGAATAAGGGA+CGG | - | chr5.3:129635-129654 | None:intergenic | 30.0% | |
| GTTGATATGTTGAATACCAT+TGG | + | chr5.3:130666-130685 | MS.gene071679:CDS | 30.0% | |
| GTTGTCATTATACAGCTTAT+GGG | + | chr5.3:130643-130662 | MS.gene071679:intron | 30.0% | |
| TCAATATAACACAAACTGGT+GGG | - | chr5.3:128809-128828 | None:intergenic | 30.0% | |
| TGTATCAAAGCATGAATAGA+CGG | - | chr5.3:129951-129970 | None:intergenic | 30.0% | |
| TTGTCATTATACAGCTTATG+GGG | + | chr5.3:130644-130663 | MS.gene071679:intron | 30.0% | |
| TTGTTTGCGAAAAAACTCAA+AGG | - | chr5.3:130082-130101 | None:intergenic | 30.0% | |
| TTTCACATTTCAAGACAAGT+TGG | - | chr5.3:129703-129722 | None:intergenic | 30.0% | |
| TTTGCCAACAAGAAATTTGA+AGG | + | chr5.3:129467-129486 | MS.gene071679:intron | 30.0% | |
| ! | CGATTTTGTGCAGTATAAAA+AGG | - | chr5.3:130014-130033 | None:intergenic | 30.0% |
| ! | TTTTATACTGCACAAAATCG+TGG | + | chr5.3:130014-130033 | MS.gene071679:intron | 30.0% |
| !! | CTTTGATACATATTTGGTGT+GGG | + | chr5.3:129961-129980 | MS.gene071679:intron | 30.0% |
| !! | GAGCTTTTTGAGGAATATAT+AGG | + | chr5.3:129266-129285 | MS.gene071679:CDS | 30.0% |
| !! | TTTGATACATATTTGGTGTG+GGG | + | chr5.3:129962-129981 | MS.gene071679:intron | 30.0% |
| !! | TTTGCTTTGCTTTAGGTTTT+GGG | + | chr5.3:129032-129051 | MS.gene071679:intron | 30.0% |
| AAAACAAAGCCTTGAGAGTA+AGG | - | chr5.3:128972-128991 | None:intergenic | 35.0% | |
| AGGCTTGTAGAGATACTAAA+AGG | + | chr5.3:130233-130252 | MS.gene071679:CDS | 35.0% | |
| AGGTAAAGGGACTTATGAAT+AGG | + | chr5.3:129829-129848 | MS.gene071679:intron | 35.0% | |
| CTAAACCACTCAGCAATAAA+AGG | - | chr5.3:130753-130772 | None:intergenic | 35.0% | |
| CTGAGTGGTTTAGAAACAAA+GGG | + | chr5.3:130760-130779 | MS.gene071679:CDS | 35.0% | |
| GACAATGGCAAAAGTTTACA+TGG | - | chr5.3:130106-130125 | None:intergenic | 35.0% | |
| GGTAAAGGGACTTATGAATA+GGG | + | chr5.3:129830-129849 | MS.gene071679:intron | 35.0% | |
| GTTAATGGTTACAGCTCATT+TGG | + | chr5.3:130426-130445 | MS.gene071679:intron | 35.0% | |
| TAATTTGAATAAGGGACGGA+GGG | - | chr5.3:129631-129650 | None:intergenic | 35.0% | |
| TCAGCTAAAATAGAAGCTGA+TGG | - | chr5.3:129411-129430 | None:intergenic | 35.0% | |
| TCATCACAAATATCAACCTG+TGG | + | chr5.3:130458-130477 | MS.gene071679:intron | 35.0% | |
| ! | AGGAAAGTATGAAAGTGGAA+AGG | - | chr5.3:130062-130081 | None:intergenic | 35.0% |
| ! | CATGGACATTTTTACTGAAG+GGG | + | chr5.3:129316-129335 | MS.gene071679:CDS | 35.0% |
| ! | CCATGGACATTTTTACTGAA+GGG | + | chr5.3:129315-129334 | MS.gene071679:CDS | 35.0% |
| ! | CTCAAAGGAAAGTATGAAAG+TGG | - | chr5.3:130067-130086 | None:intergenic | 35.0% |
| ! | GATGATGATGATGATGATGA+TGG | + | chr5.3:128827-128846 | MS.gene071679:intron | 35.0% |
| ! | GCTTTGATACATATTTGGTG+TGG | + | chr5.3:129960-129979 | MS.gene071679:intron | 35.0% |
| ! | TCCATGGACATTTTTACTGA+AGG | + | chr5.3:129314-129333 | MS.gene071679:CDS | 35.0% |
| ! | TTTTTACAAGCTACAACCAC+AGG | - | chr5.3:130477-130496 | None:intergenic | 35.0% |
| !! | CTTTGCTTTGCTTTAGGTTT+TGG | + | chr5.3:129031-129050 | MS.gene071679:intron | 35.0% |
| !! | GCAGGGCTTTTTAGTATTTT+CGG | + | chr5.3:129897-129916 | MS.gene071679:intron | 35.0% |
| !!! | AGGGAAGAAAGCGATTTTTT+TGG | + | chr5.3:130722-130741 | MS.gene071679:CDS | 35.0% |
| !!! | GGGAAGAAAGCGATTTTTTT+GGG | + | chr5.3:130723-130742 | MS.gene071679:CDS | 35.0% |
| !!! | TTTTGGTAGTGAGCTTTTTG+AGG | + | chr5.3:129256-129275 | MS.gene071679:CDS | 35.0% |
| AAGCTCACTACCAAAAAGCA+TGG | - | chr5.3:129252-129271 | None:intergenic | 40.0% | |
| AAGGGACTTATGAATAGGGA+CGG | + | chr5.3:129834-129853 | MS.gene071679:intron | 40.0% | |
| ACTGTTCATCCTTACTCTCA+AGG | + | chr5.3:128960-128979 | MS.gene071679:intron | 40.0% | |
| ATATAACACAAACTGGTGGG+TGG | - | chr5.3:128806-128825 | None:intergenic | 40.0% | |
| ATATATAGGCCAGTTAGCCA+TGG | + | chr5.3:129280-129299 | MS.gene071679:CDS | 40.0% | |
| CAAAGCAGTGGTAGATAGAT+GGG | - | chr5.3:129006-129025 | None:intergenic | 40.0% | |
| CATCAAATCCCAAGTTACAG+CGG | + | chr5.3:130788-130807 | MS.gene071679:CDS | 40.0% | |
| CCCTTCAGTAAAAATGTCCA+TGG | - | chr5.3:129318-129337 | None:intergenic | 40.0% | |
| CTTAATCTCAGATTCGGATG+CGG | - | chr5.3:128714-128733 | None:intergenic | 40.0% | |
| GATTGCAGCAGGATCAATAA+TGG | - | chr5.3:129225-129244 | None:intergenic | 40.0% | |
| GCTGAGTGGTTTAGAAACAA+AGG | + | chr5.3:130759-130778 | MS.gene071679:CDS | 40.0% | |
| GCTTTGCTTTGCTTTGCTTT+AGG | + | chr5.3:129020-129039 | MS.gene071679:intron | 40.0% | |
| GTAATTTGAATAAGGGACGG+AGG | - | chr5.3:129632-129651 | None:intergenic | 40.0% | |
| TATGATGCACATGGCAAATC+TGG | + | chr5.3:129098-129117 | MS.gene071679:CDS | 40.0% | |
| TCACTGCAAAGAAAGGAAGA+AGG | - | chr5.3:129200-129219 | None:intergenic | 40.0% | |
| TCATAAGCTTGCCTTTGAGT+TGG | - | chr5.3:129083-129102 | None:intergenic | 40.0% | |
| TTCAGAAGGAGAGAGAAGAA+AGG | + | chr5.3:130213-130232 | MS.gene071679:CDS | 40.0% | |
| ! | CAAAGTCTTCTTTGTTTCCC+TGG | - | chr5.3:130277-130296 | None:intergenic | 40.0% |
| ! | CATAAGCTTGCCTTTGAGTT+GGG | - | chr5.3:129082-129101 | None:intergenic | 40.0% |
| ! | GAAAATTTTGGGCAGCAAGA+GGG | - | chr5.3:128913-128932 | None:intergenic | 40.0% |
| ! | GCAATCTTTGCCATGCTTTT+TGG | + | chr5.3:129239-129258 | MS.gene071679:CDS | 40.0% |
| ! | TATAACACAAACTGGTGGGT+GGG | - | chr5.3:128805-128824 | None:intergenic | 40.0% |
| ! | TCTTGCTGCCCAAAATTTTC+AGG | + | chr5.3:128913-128932 | MS.gene071679:CDS | 40.0% |
| ! | TGAAAATTTTGGGCAGCAAG+AGG | - | chr5.3:128914-128933 | None:intergenic | 40.0% |
| !! | GGGTCATTTGGGTTTTTATC+CGG | - | chr5.3:128893-128912 | None:intergenic | 40.0% |
| AACTACCTTCGAGACGACAA+TGG | - | chr5.3:130121-130140 | None:intergenic | 45.0% | |
| AATGGCGTCACTGCAAAGAA+AGG | - | chr5.3:129207-129226 | None:intergenic | 45.0% | |
| AGACTCAACCAGTATGTCCA+GGG | + | chr5.3:130257-130276 | MS.gene071679:CDS | 45.0% | |
| AGCAAAGCAAAGCAAAGCAG+TGG | - | chr5.3:129018-129037 | None:intergenic | 45.0% | |
| AGCGAATACTATGACGTGCT+TGG | + | chr5.3:128677-128696 | MS.gene071679:CDS | 45.0% | |
| AGGCAAGCTTATGATGCACA+TGG | + | chr5.3:129089-129108 | MS.gene071679:CDS | 45.0% | |
| CCTTGCGTATATGTAGCCAA+TGG | - | chr5.3:130685-130704 | None:intergenic | 45.0% | |
| GCAAAGCAGTGGTAGATAGA+TGG | - | chr5.3:129007-129026 | None:intergenic | 45.0% | |
| TTCTCAGTGACCCAACTCAA+AGG | + | chr5.3:129069-129088 | MS.gene071679:CDS | 45.0% | |
| ! | CCATTGGCTACATATACGCA+AGG | + | chr5.3:130682-130701 | MS.gene071679:CDS | 45.0% |
| ! | CTTTGTTTCCCTGGACATAC+TGG | - | chr5.3:130268-130287 | None:intergenic | 45.0% |
| ! | GTGTGCCTTTTATTGCTGAG+TGG | + | chr5.3:130745-130764 | MS.gene071679:CDS | 45.0% |
| ! | TTTTGCCATTGTCGTCTCGA+AGG | + | chr5.3:130113-130132 | MS.gene071679:intron | 45.0% |
| !!! | TTTGCTTTAGGTTTTGGGCG+AGG | + | chr5.3:129037-129056 | MS.gene071679:intron | 45.0% |
| !!! | TTTTAATTAATTATTCAATT+CGG | + | chr5.3:129163-129182 | MS.gene071679:intron | 5.0% |
| AGCATGGCAAAGATTGCAGC+AGG | - | chr5.3:129236-129255 | None:intergenic | 50.0% | |
| ATCTCAGATTCGGATGCGGT+TGG | - | chr5.3:128710-128729 | None:intergenic | 50.0% | |
| ATGCAGAAGCTGAAGTAGCC+CGG | + | chr5.3:130303-130322 | MS.gene071679:CDS | 50.0% | |
| CAGACTCAACCAGTATGTCC+AGG | + | chr5.3:130256-130275 | MS.gene071679:CDS | 50.0% | |
| CATGGAAGCCATTGATGCCA+TGG | - | chr5.3:129300-129319 | None:intergenic | 50.0% | |
| CATGGCATCAATGGCTTCCA+TGG | + | chr5.3:129298-129317 | MS.gene071679:CDS | 50.0% | |
| CCAGTTAGCCATGGCATCAA+TGG | + | chr5.3:129289-129308 | MS.gene071679:CDS | 50.0% | |
| CCATTGATGCCATGGCTAAC+TGG | - | chr5.3:129292-129311 | None:intergenic | 50.0% | |
| CTCTCTCCTTCTGAACAACC+TGG | - | chr5.3:130208-130227 | None:intergenic | 50.0% | |
| GAGTTGGGTCACTGAGAACT+TGG | - | chr5.3:129067-129086 | None:intergenic | 50.0% | |
| TCTCCTTCTGAACAACCTGG+CGG | - | chr5.3:130205-130224 | None:intergenic | 50.0% | |
| TGGCTACATATACGCAAGGC+AGG | + | chr5.3:130686-130705 | MS.gene071679:CDS | 50.0% | |
| !! | TTAGGGCATCGTACCTGCAT+TGG | - | chr5.3:130333-130352 | None:intergenic | 50.0% |
| ACAGGCAAGGCAAGTGCATC+CGG | + | chr5.3:128871-128890 | MS.gene071679:intron | 55.0% | |
| AGGCAGGCAGCAAAAGAGCT+AGG | + | chr5.3:130702-130721 | MS.gene071679:CDS | 55.0% | |
| CTTCCGCCAGGTTGTTCAGA+AGG | + | chr5.3:130199-130218 | MS.gene071679:intron | 55.0% | |
| GGCAGGCAGCAAAAGAGCTA+GGG | + | chr5.3:130703-130722 | MS.gene071679:CDS | 55.0% | |
| GGGCAGCAAGAGGGTCATTT+GGG | - | chr5.3:128904-128923 | None:intergenic | 55.0% | |
| TCGTACCTGCATTGGACAGC+CGG | - | chr5.3:130325-130344 | None:intergenic | 55.0% | |
| TGGGCAGCAAGAGGGTCATT+TGG | - | chr5.3:128905-128924 | None:intergenic | 55.0% | |
| ! | AACACAAACTGGTGGGTGGG+TGG | - | chr5.3:128802-128821 | None:intergenic | 55.0% |
| CGTACCTGCATTGGACAGCC+GGG | - | chr5.3:130324-130343 | None:intergenic | 60.0% | |
| GTAGCCCGGCTGTCCAATGC+AGG | + | chr5.3:130317-130336 | MS.gene071679:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 128665 | 130816 | 128665 | ID=MS.gene071679 |
| chr5.3 | mRNA | 128665 | 130816 | 128665 | ID=MS.gene071679.t1;Parent=MS.gene071679 |
| chr5.3 | exon | 128665 | 128751 | 128665 | ID=MS.gene071679.t1.exon1;Parent=MS.gene071679.t1 |
| chr5.3 | CDS | 128665 | 128751 | 128665 | ID=cds.MS.gene071679.t1;Parent=MS.gene071679.t1 |
| chr5.3 | exon | 128875 | 128934 | 128875 | ID=MS.gene071679.t1.exon2;Parent=MS.gene071679.t1 |
| chr5.3 | CDS | 128875 | 128934 | 128875 | ID=cds.MS.gene071679.t1;Parent=MS.gene071679.t1 |
| chr5.3 | exon | 129047 | 129129 | 129047 | ID=MS.gene071679.t1.exon3;Parent=MS.gene071679.t1 |
| chr5.3 | CDS | 129047 | 129129 | 129047 | ID=cds.MS.gene071679.t1;Parent=MS.gene071679.t1 |
| chr5.3 | exon | 129217 | 129376 | 129217 | ID=MS.gene071679.t1.exon4;Parent=MS.gene071679.t1 |
| chr5.3 | CDS | 129217 | 129376 | 129217 | ID=cds.MS.gene071679.t1;Parent=MS.gene071679.t1 |
| chr5.3 | exon | 130209 | 130338 | 130209 | ID=MS.gene071679.t1.exon5;Parent=MS.gene071679.t1 |
| chr5.3 | CDS | 130209 | 130338 | 130209 | ID=cds.MS.gene071679.t1;Parent=MS.gene071679.t1 |
| chr5.3 | exon | 130658 | 130816 | 130658 | ID=MS.gene071679.t1.exon6;Parent=MS.gene071679.t1 |
| chr5.3 | CDS | 130658 | 130816 | 130658 | ID=cds.MS.gene071679.t1;Parent=MS.gene071679.t1 |
| Gene Sequence |
| Protein sequence |
