AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072418

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072418.t1 MTR_1g096310 89.516 372 4 2 1 337 1 372 0.0 665
MS.gene072418.t1 MTR_7g011900 57.909 373 118 5 1 337 1 370 8.81e-147 419
MS.gene072418.t1 MTR_7g012040 57.260 365 118 4 8 337 7 368 4.83e-142 406
MS.gene072418.t1 MTR_7g012020 55.464 366 124 5 8 337 7 369 1.29e-137 395
MS.gene072418.t1 MTR_7g011990 53.678 367 126 5 8 337 8 367 5.10e-132 381
MS.gene072418.t1 MTR_7g012070 51.862 376 141 5 1 337 1 375 8.13e-130 375
MS.gene072418.t1 MTR_7g012090 52.394 376 139 5 1 337 1 375 1.23e-129 375
MS.gene072418.t1 MTR_2g055940 54.391 353 121 5 21 337 15 363 2.01e-128 372
MS.gene072418.t1 MTR_3g021430 50.416 361 139 6 13 337 1 357 5.76e-113 332
MS.gene072418.t1 MTR_3g021440 49.716 352 139 5 21 337 22 370 1.34e-111 329
MS.gene072418.t1 MTR_7g012020 59.410 271 103 4 8 277 7 271 8.24e-109 319
MS.gene072418.t1 MTR_7g012020 63.265 245 86 3 8 251 7 248 2.90e-108 316
MS.gene072418.t1 MTR_1g036460 46.216 370 156 8 7 337 5 370 1.93e-103 308
MS.gene072418.t1 MTR_1g036490 44.837 368 161 7 9 337 6 370 6.26e-102 304
MS.gene072418.t1 MTR_1g036510 44.772 373 156 8 12 337 15 384 3.86e-98 295
MS.gene072418.t1 MTR_3g092900 43.407 364 164 8 10 337 6 363 1.74e-89 273
MS.gene072418.t1 MTR_5g098170 38.418 354 178 7 20 337 14 363 3.81e-71 225
MS.gene072418.t1 MTR_1g036510 48.594 249 114 5 12 249 15 260 3.16e-70 220
MS.gene072418.t1 MTR_1g036510 48.594 249 114 5 12 249 15 260 1.04e-69 219
MS.gene072418.t1 MTR_1g050220 40.248 323 124 7 26 320 6 287 7.05e-68 215
MS.gene072418.t1 MTR_7g118300 36.885 366 189 8 8 337 16 375 3.59e-63 205
MS.gene072418.t1 MTR_0591s0010 35.180 361 186 9 18 337 24 377 1.30e-58 193
MS.gene072418.t1 MTR_7g118320 33.515 367 194 9 13 337 4 362 2.27e-57 190
MS.gene072418.t1 MTR_8g068690 34.540 359 191 9 18 337 23 376 4.15e-56 187
MS.gene072418.t1 MTR_8g068750 33.983 359 193 9 18 337 24 377 8.03e-55 184
MS.gene072418.t1 MTR_8g068870 33.607 366 199 8 11 337 3 363 4.61e-52 176
MS.gene072418.t1 MTR_8g024160 30.986 355 163 7 19 337 20 328 1.32e-44 155
MS.gene072418.t1 MTR_6g060290 47.802 182 73 6 8 186 8 170 2.49e-44 150
MS.gene072418.t1 MTR_8g068690 37.872 235 139 5 18 249 23 253 3.86e-44 153
MS.gene072418.t1 MTR_6g078850 30.831 373 215 7 3 336 14 382 8.84e-44 157
MS.gene072418.t1 MTR_8g068570 32.911 316 149 7 18 301 24 308 1.94e-43 152
MS.gene072418.t1 MTR_3g096050 32.800 375 188 14 9 337 2 358 1.20e-41 149
MS.gene072418.t1 MTR_8g068730 30.362 359 174 9 18 337 24 345 1.15e-38 140
MS.gene072418.t1 MTR_2g072790 29.799 349 195 8 13 321 1 339 1.59e-38 140
MS.gene072418.t1 MTR_8g068600 37.073 205 123 4 47 249 28 228 6.48e-37 134
MS.gene072418.t1 MTR_3g096050 36.437 247 137 8 9 249 2 234 6.08e-34 125
MS.gene072418.t1 MTR_3g096070 30.831 373 175 16 9 337 2 335 1.75e-31 121
MS.gene072418.t1 MTR_0017s0100 29.052 327 171 13 41 322 44 354 3.15e-25 104
MS.gene072418.t1 MTR_5g074600 23.795 332 200 10 34 323 26 346 1.28e-24 102
MS.gene072418.t1 MTR_8g068740 28.716 296 166 8 18 310 24 277 1.42e-24 101
MS.gene072418.t1 MTR_0079s0070 28.793 323 177 10 41 322 10 320 1.62e-24 102
MS.gene072418.t1 MTR_4g038440 28.793 323 177 10 41 322 44 354 2.42e-24 102
MS.gene072418.t1 MTR_8g068620 51.190 84 41 0 166 249 2 85 4.02e-24 95.5
MS.gene072418.t1 MTR_5g074680 26.205 332 192 10 34 323 27 347 5.83e-24 101
MS.gene072418.t1 MTR_4g033200 26.347 334 199 10 13 306 1 327 2.80e-22 96.3
MS.gene072418.t1 MTR_3g083620 25.077 323 190 9 39 322 38 347 4.98e-22 95.9
MS.gene072418.t1 MTR_5g074580 25.602 332 194 11 34 323 27 347 5.94e-22 95.5
MS.gene072418.t1 MTR_4g033015 25.749 334 201 9 13 306 1 327 2.05e-21 94.0
MS.gene072418.t1 MTR_5g074540 25.507 345 185 13 34 336 26 340 2.68e-21 93.2
MS.gene072418.t1 MTR_7g014510 24.611 321 195 9 41 323 33 344 1.71e-19 88.6
MS.gene072418.t1 MTR_4g088345 24.383 324 195 8 38 322 30 342 3.83e-19 87.4
MS.gene072418.t1 MTR_4g033150 24.773 331 202 9 16 306 4 327 4.12e-19 87.4
MS.gene072418.t1 MTR_4g038440 31.776 214 129 7 41 249 44 245 7.60e-19 85.5
MS.gene072418.t1 MTR_1g076940 24.918 305 184 7 41 309 33 328 1.32e-18 85.9
MS.gene072418.t1 MTR_4g079160 25.424 354 198 15 19 322 4 341 1.95e-18 85.5
MS.gene072418.t1 MTR_4g088200 27.647 340 194 12 34 330 26 356 2.72e-18 85.1
MS.gene072418.t1 MTR_0794s0020 26.877 253 167 8 16 260 4 246 1.98e-17 80.9
MS.gene072418.t1 MTR_7g014570 24.390 328 181 9 41 317 33 344 4.90e-17 81.3
MS.gene072418.t1 MTR_7g024500 24.390 328 185 11 38 322 29 336 7.62e-17 80.9
MS.gene072418.t1 MTR_1g076930 26.047 215 144 6 38 248 7 210 1.38e-16 78.6
MS.gene072418.t1 MTR_7g024520 24.398 332 188 11 34 322 25 336 1.71e-16 79.7
MS.gene072418.t1 MTR_5g074610 22.985 335 170 12 34 323 22 313 1.60e-14 73.6
MS.gene072418.t1 MTR_4g033170 26.389 288 164 9 17 268 5 280 3.58e-14 72.8
MS.gene072418.t1 MTR_4g088190 26.744 344 195 11 34 330 26 359 5.24e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072418.t1 AT5G54160 41.860 344 158 6 20 327 15 352 4.81e-82 253
MS.gene072418.t1 AT1G51990 35.537 363 190 8 12 336 5 361 8.71e-65 209
MS.gene072418.t1 AT1G51990 35.537 363 190 8 12 336 5 361 7.37e-64 207
MS.gene072418.t1 AT1G77520 35.372 376 188 11 6 337 17 381 1.26e-62 204
MS.gene072418.t1 AT1G63140 34.903 361 186 10 21 337 26 381 1.78e-54 183
MS.gene072418.t1 AT1G63140 34.903 361 186 10 21 337 46 401 2.50e-54 183
MS.gene072418.t1 AT1G33030 35.143 350 178 10 28 337 10 350 6.30e-54 181
MS.gene072418.t1 AT1G77530 35.561 374 190 10 6 337 17 381 2.63e-51 174
MS.gene072418.t1 AT1G21130 34.844 353 183 11 26 337 27 373 3.73e-50 171
MS.gene072418.t1 AT1G21110 34.561 353 184 11 26 337 27 373 5.25e-49 168
MS.gene072418.t1 AT5G53810 34.795 342 179 8 38 337 39 378 1.61e-48 167
MS.gene072418.t1 AT1G21100 34.561 353 184 11 26 337 27 373 3.17e-48 166
MS.gene072418.t1 AT1G21120 33.994 353 186 11 26 337 27 373 2.64e-47 164
MS.gene072418.t1 AT1G21120 33.994 353 186 11 26 337 75 421 5.63e-47 164
MS.gene072418.t1 AT1G33030 36.508 252 149 6 28 277 10 252 5.14e-42 147
MS.gene072418.t1 AT1G33030 36.508 252 149 6 28 277 10 252 1.04e-41 146
MS.gene072418.t1 AT1G76790 32.493 357 188 9 26 337 17 365 1.72e-41 148
MS.gene072418.t1 AT1G63140 37.398 246 137 6 21 253 46 287 2.09e-39 141
MS.gene072418.t1 AT3G53140 31.392 309 171 6 17 291 5 306 8.27e-39 141
MS.gene072418.t1 AT1G63140 37.288 236 137 5 21 249 46 277 1.75e-38 139
MS.gene072418.t1 AT1G77530 38.554 249 140 5 6 249 17 257 4.02e-38 137
MS.gene072418.t1 AT5G37170 32.515 326 142 10 56 337 43 334 1.53e-37 137
MS.gene072418.t1 AT1G21130 40.351 228 127 6 26 249 27 249 7.47e-36 132
MS.gene072418.t1 AT1G62900 36.946 203 90 5 172 337 4 205 5.14e-31 116
MS.gene072418.t1 AT5G37170 35.859 198 88 5 178 337 38 234 4.52e-28 109
MS.gene072418.t1 AT5G37170 35.829 187 81 5 189 337 1 186 4.30e-25 100
MS.gene072418.t1 AT4G35150 29.817 218 98 9 162 330 105 316 1.29e-20 91.3
MS.gene072418.t1 AT4G35160 26.902 368 199 14 9 322 16 367 2.22e-20 91.3

Find 67 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTTTATTAATGGATATT+TGG 0.122440 1.1:-69388736 None:intergenic
AAGTTAATTCCCTTTATTAA+TGG 0.172707 1.1:-69388744 None:intergenic
TCACTTGTGTTTGGTTCTTC+TGG 0.194077 1.1:-69389240 None:intergenic
TGATGAAAGTAGAGGTTATT+TGG 0.221768 1.1:+69388171 MS.gene072418:CDS
GAAAATGCACCACCACTTTC+AGG 0.245970 1.1:+69388786 MS.gene072418:CDS
ACGGCAAGCAACTTGAAATT+TGG 0.263471 1.1:-69389352 None:intergenic
ATCCAAATATCCATTAATAA+AGG 0.266045 1.1:+69388734 MS.gene072418:CDS
ATCCTGCATTATTACAAGTT+TGG 0.305992 1.1:+69388218 MS.gene072418:CDS
TGCCGTGCTTTCAACAGTTT+GGG 0.308799 1.1:+69389369 MS.gene072418:CDS
TTGCCGTGCTTTCAACAGTT+TGG 0.318040 1.1:+69389368 MS.gene072418:CDS
TTAGATTCTTCACTTGTGTT+TGG 0.326373 1.1:-69389249 None:intergenic
GACTTGTTCAAGAACGTTCA+TGG 0.340035 1.1:+69388531 MS.gene072418:CDS
GTTCTGAGGTAGCACTAATT+TGG 0.352301 1.1:-69387829 None:intergenic
GGTGGTGCCGAGAGAGTTTA+CGG 0.386591 1.1:+69388118 MS.gene072418:CDS
AGCACTCGGACTTGCCTAAT+AGG 0.390804 1.1:+69388026 MS.gene072418:CDS
AGTGACAAAGTATGAATTCA+TGG 0.400109 1.1:+69388554 MS.gene072418:CDS
GTTATCCTCCTTGGTAATGT+AGG 0.401834 1.1:-69387807 None:intergenic
ACTAATTTGGTTATCCTCCT+TGG 0.402910 1.1:-69387816 None:intergenic
TCCAAATATCCATTAATAAA+GGG 0.404369 1.1:+69388735 MS.gene072418:CDS
AGCATGCGGTCAAGCCTATT+AGG 0.413248 1.1:-69388040 None:intergenic
GAGAAATTGAGCAAACTCTC+TGG 0.417488 1.1:+69389324 MS.gene072418:CDS
TGAAATATACACTGGATTTG+AGG 0.429712 1.1:+69388653 MS.gene072418:CDS
CACAAAGCTTTATCACCAAA+TGG 0.430052 1.1:+69389189 MS.gene072418:CDS
AAATAACCTCTACTTTCATC+AGG 0.441916 1.1:-69388169 None:intergenic
GAAATATACACTGGATTTGA+GGG 0.446533 1.1:+69388654 MS.gene072418:CDS
GTTGATGTTGGAGGTGGAAG+TGG 0.451719 1.1:+69388690 MS.gene072418:CDS
ATAGCAGCATTTAACACTGC+TGG 0.468445 1.1:-69387908 None:intergenic
CTCATGTTTATCACAGTTGG+TGG 0.480360 1.1:+69389294 MS.gene072418:CDS
ATATCAACATTAGTTGATGT+TGG 0.501358 1.1:+69388678 MS.gene072418:CDS
ACATTAGTTGATGTTGGAGG+TGG 0.504612 1.1:+69388684 MS.gene072418:CDS
GAGAGTTTACGGACTCTCAA+TGG 0.504711 1.1:+69388129 MS.gene072418:CDS
AATCTCATGTTTATCACAGT+TGG 0.506086 1.1:+69389291 MS.gene072418:CDS
CTCGGCACCACCATCCTCAA+TGG 0.508530 1.1:-69388107 None:intergenic
TGCTGGATAAACAAGATTAG+TGG 0.509486 1.1:-69387891 None:intergenic
TTTATTTGAGATCATAGCTA+AGG 0.511778 1.1:+69387940 MS.gene072418:CDS
TTTCAACAGTTTGGGAGTGA+TGG 0.524643 1.1:+69389377 MS.gene072418:CDS
ATCCTCAATGGTTCGAGTAG+TGG 0.527799 1.1:-69388095 None:intergenic
TGTTTATCACAGTTGGTGGA+AGG 0.529690 1.1:+69389298 MS.gene072418:CDS
TCCGAGTGCTGCGTTGATGC+TGG 0.530284 1.1:-69388013 None:intergenic
TACCAAACTTGTAATAATGC+AGG 0.531997 1.1:-69388220 None:intergenic
GTTTATCACAGTTGGTGGAA+GGG 0.543556 1.1:+69389299 MS.gene072418:CDS
AAAGTATGAATTCATGGGAA+AGG 0.556043 1.1:+69388560 MS.gene072418:CDS
ACCAGCATCAACGCAGCACT+CGG 0.557596 1.1:+69388012 MS.gene072418:CDS
TTCCACTACTCGAACCATTG+AGG 0.558053 1.1:+69388093 MS.gene072418:CDS
TATCATGTTATACCTGAAAG+TGG 0.563244 1.1:-69388798 None:intergenic
GTGACAAAGTATGAATTCAT+GGG 0.563593 1.1:+69388555 MS.gene072418:CDS
ATAGCTAAGGCAACACCACC+TGG 0.567142 1.1:+69387953 MS.gene072418:CDS
TCAACATTAGTTGATGTTGG+AGG 0.568137 1.1:+69388681 MS.gene072418:CDS
CTCCCAAACTGTTGAAAGCA+CGG 0.568203 1.1:-69389371 None:intergenic
AATTTAGAAGCAATTTCAGA+TGG 0.568884 1.1:-69387989 None:intergenic
TGAGAGTCCGTAAACTCTCT+CGG 0.571590 1.1:-69388125 None:intergenic
AGAATGCTTGAAATATACAC+TGG 0.575900 1.1:+69388645 MS.gene072418:CDS
CTCTACTTTCATCAGGGACA+AGG 0.594593 1.1:-69388162 None:intergenic
ACTGTCTTCAGTTTGTTCTG+AGG 0.597743 1.1:-69387843 None:intergenic
CAGTGCATGTCTTTCAGCAA+TGG 0.602261 1.1:+69387862 MS.gene072418:CDS
CTTGTCCCTGATGAAAGTAG+AGG 0.618925 1.1:+69388163 MS.gene072418:CDS
AAATGGAAAAGTGATTATTG+TGG 0.620942 1.1:+69389206 MS.gene072418:CDS
GATGGTGACATGAAAGCACC+AGG 0.624085 1.1:-69387971 None:intergenic
GTTTACGGACTCTCAATGGT+CGG 0.636343 1.1:+69388133 MS.gene072418:CDS
ACTACTCGAACCATTGAGGA+TGG 0.639748 1.1:+69388097 MS.gene072418:CDS
TAGCAAGCAAACGCAGCATG+CGG 0.640854 1.1:-69388054 None:intergenic
GGGAAATTCCTACATTACCA+AGG 0.642464 1.1:+69387799 MS.gene072418:CDS
AATAACCTCTACTTTCATCA+GGG 0.648899 1.1:-69388168 None:intergenic
ACTCGAACCATTGAGGATGG+TGG 0.675966 1.1:+69388100 MS.gene072418:CDS
AAATTCCTACATTACCAAGG+AGG 0.730361 1.1:+69387802 MS.gene072418:CDS
CATGTTATACCTGAAAGTGG+TGG 0.738842 1.1:-69388795 None:intergenic
GGTGACATGAAAGCACCAGG+TGG 0.810388 1.1:-69387968 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATACATATATAAAATCAA+GGG + chr1.1:69388263-69388282 MS.gene072418:intron 15.0%
!! AAGTTAATTCCCTTTATTAA+TGG - chr1.1:69388747-69388766 None:intergenic 20.0%
!! AGTAAAACTTTAGTATAAGA+TGG - chr1.1:69388862-69388881 None:intergenic 20.0%
!! ATATTCACCTATTAAACATA+TGG + chr1.1:69388315-69388334 MS.gene072418:intron 20.0%
!! ATCCAAATATCCATTAATAA+AGG + chr1.1:69388734-69388753 MS.gene072418:CDS 20.0%
!! CTCATACATATATAAAATCA+AGG + chr1.1:69388262-69388281 MS.gene072418:intron 20.0%
!! TCCAAATATCCATTAATAAA+GGG + chr1.1:69388735-69388754 MS.gene072418:CDS 20.0%
!!! CCAAATTTTTAACAAATCAA+TGG + chr1.1:69388596-69388615 MS.gene072418:CDS 20.0%
!!! CCATTGATTTGTTAAAAATT+TGG - chr1.1:69388599-69388618 None:intergenic 20.0%
! AAATGGAAAAGTGATTATTG+TGG + chr1.1:69389206-69389225 MS.gene072418:CDS 25.0%
! AATTTAGAAGCAATTTCAGA+TGG - chr1.1:69387992-69388011 None:intergenic 25.0%
! ATATCCTCCATATGTTTAAT+AGG - chr1.1:69388325-69388344 None:intergenic 25.0%
! GGAGGATATATAAGTTATAA+AGG + chr1.1:69388336-69388355 MS.gene072418:intron 25.0%
! TTAAGCTAATTGTAGCAATT+AGG + chr1.1:69388418-69388437 MS.gene072418:intron 25.0%
!! ATATCAACATTAGTTGATGT+TGG + chr1.1:69388678-69388697 MS.gene072418:CDS 25.0%
!! ATTTTTCATCTGACCAATTA+TGG - chr1.1:69389146-69389165 None:intergenic 25.0%
!! GACTTTGTTTACATAATTGT+AGG + chr1.1:69389113-69389132 MS.gene072418:intron 25.0%
!! TCCCTTTATTAATGGATATT+TGG - chr1.1:69388739-69388758 None:intergenic 25.0%
!! TTTATTTGAGATCATAGCTA+AGG + chr1.1:69387940-69387959 MS.gene072418:CDS 25.0%
!!! ACAATAATCACTTTTCCATT+TGG - chr1.1:69389207-69389226 None:intergenic 25.0%
!!! TCTTACTTTTGTTCCAATTA+TGG + chr1.1:69388448-69388467 MS.gene072418:intron 25.0%
AAAGTATGAATTCATGGGAA+AGG + chr1.1:69388560-69388579 MS.gene072418:CDS 30.0%
AAATAACCTCTACTTTCATC+AGG - chr1.1:69388172-69388191 None:intergenic 30.0%
AATAACCTCTACTTTCATCA+GGG - chr1.1:69388171-69388190 None:intergenic 30.0%
AATCTCATGTTTATCACAGT+TGG + chr1.1:69389291-69389310 MS.gene072418:CDS 30.0%
ACTAAAGCTTAGACCATAAT+TGG - chr1.1:69388464-69388483 None:intergenic 30.0%
AGAATGCTTGAAATATACAC+TGG + chr1.1:69388645-69388664 MS.gene072418:CDS 30.0%
AGTGACAAAGTATGAATTCA+TGG + chr1.1:69388554-69388573 MS.gene072418:CDS 30.0%
ATCCTGCATTATTACAAGTT+TGG + chr1.1:69388218-69388237 MS.gene072418:CDS 30.0%
GAAATATACACTGGATTTGA+GGG + chr1.1:69388654-69388673 MS.gene072418:CDS 30.0%
GTGACAAAGTATGAATTCAT+GGG + chr1.1:69388555-69388574 MS.gene072418:CDS 30.0%
TACCAAACTTGTAATAATGC+AGG - chr1.1:69388223-69388242 None:intergenic 30.0%
TATACTTACCTTCAGTATCA+TGG - chr1.1:69388988-69389007 None:intergenic 30.0%
TATCATGTTATACCTGAAAG+TGG - chr1.1:69388801-69388820 None:intergenic 30.0%
TGAAATATACACTGGATTTG+AGG + chr1.1:69388653-69388672 MS.gene072418:CDS 30.0%
TGATGAAAGTAGAGGTTATT+TGG + chr1.1:69388171-69388190 MS.gene072418:CDS 30.0%
TTAGATTCTTCACTTGTGTT+TGG - chr1.1:69389252-69389271 None:intergenic 30.0%
TTCACCTATTAAACATATGG+AGG + chr1.1:69388318-69388337 MS.gene072418:intron 30.0%
! ATACCACCAACTTTTGTTAA+GGG + chr1.1:69388913-69388932 MS.gene072418:intron 30.0%
! TATACCACCAACTTTTGTTA+AGG + chr1.1:69388912-69388931 MS.gene072418:intron 30.0%
! TCTATCCAGGATGAATTTTA+AGG + chr1.1:69388485-69388504 MS.gene072418:intron 30.0%
! TGGTGCATTTTCAATAACTT+GGG - chr1.1:69388778-69388797 None:intergenic 30.0%
AAAATCAAGGGATAGTGACT+TGG + chr1.1:69388275-69388294 MS.gene072418:intron 35.0%
AAATTCCTACATTACCAAGG+AGG + chr1.1:69387802-69387821 MS.gene072418:CDS 35.0%
AATCCCTTAACAAAAGTTGG+TGG - chr1.1:69388919-69388938 None:intergenic 35.0%
ACTAATTTGGTTATCCTCCT+TGG - chr1.1:69387819-69387838 None:intergenic 35.0%
CTCAATCCCTTAACAAAAGT+TGG - chr1.1:69388922-69388941 None:intergenic 35.0%
TGCTGGATAAACAAGATTAG+TGG - chr1.1:69387894-69387913 None:intergenic 35.0%
! CACAAAGCTTTATCACCAAA+TGG + chr1.1:69389189-69389208 MS.gene072418:CDS 35.0%
! CTAAGCTTTAGTTTCTATCC+AGG + chr1.1:69388472-69388491 MS.gene072418:intron 35.0%
! GGTGCATTTTCAATAACTTG+GGG - chr1.1:69388777-69388796 None:intergenic 35.0%
! GTGGTGCATTTTCAATAACT+TGG - chr1.1:69388779-69388798 None:intergenic 35.0%
! TCAACATTAGTTGATGTTGG+AGG + chr1.1:69388681-69388700 MS.gene072418:CDS 35.0%
!! CAGGATGAATTTTAAGGAAG+CGG + chr1.1:69388491-69388510 MS.gene072418:intron 35.0%
ACGGCAAGCAACTTGAAATT+TGG - chr1.1:69389355-69389374 None:intergenic 40.0%
ATAGCAGCATTTAACACTGC+TGG - chr1.1:69387911-69387930 None:intergenic 40.0%
ATGTTTGCAAGTGTTCCACA+GGG + chr1.1:69388957-69388976 MS.gene072418:intron 40.0%
CATGTTATACCTGAAAGTGG+TGG - chr1.1:69388798-69388817 None:intergenic 40.0%
CGCTTCCTTAAAATTCATCC+TGG - chr1.1:69388493-69388512 None:intergenic 40.0%
CTCATGTTTATCACAGTTGG+TGG + chr1.1:69389294-69389313 MS.gene072418:CDS 40.0%
GAGAAATTGAGCAAACTCTC+TGG + chr1.1:69389324-69389343 MS.gene072418:CDS 40.0%
GGGAAATTCCTACATTACCA+AGG + chr1.1:69387799-69387818 MS.gene072418:CDS 40.0%
TATGTTTGCAAGTGTTCCAC+AGG + chr1.1:69388956-69388975 MS.gene072418:intron 40.0%
TGTAGGCTGTATGCCATAAT+TGG + chr1.1:69389130-69389149 MS.gene072418:intron 40.0%
TGTTAAGGGATTGAGCATGT+TGG + chr1.1:69388927-69388946 MS.gene072418:intron 40.0%
TTTCAACAGTTTGGGAGTGA+TGG + chr1.1:69389377-69389396 MS.gene072418:CDS 40.0%
! ACATTAGTTGATGTTGGAGG+TGG + chr1.1:69388684-69388703 MS.gene072418:CDS 40.0%
! ACTGTCTTCAGTTTGTTCTG+AGG - chr1.1:69387846-69387865 None:intergenic 40.0%
! GACTTGTTCAAGAACGTTCA+TGG + chr1.1:69388531-69388550 MS.gene072418:CDS 40.0%
!! GATGAATTTTAAGGAAGCGG+TGG + chr1.1:69388494-69388513 MS.gene072418:CDS 40.0%
!! GTTATCCTCCTTGGTAATGT+AGG - chr1.1:69387810-69387829 None:intergenic 40.0%
!! GTTCTGAGGTAGCACTAATT+TGG - chr1.1:69387832-69387851 None:intergenic 40.0%
!! GTTTATCACAGTTGGTGGAA+GGG + chr1.1:69389299-69389318 MS.gene072418:CDS 40.0%
!! TCACTTGTGTTTGGTTCTTC+TGG - chr1.1:69389243-69389262 None:intergenic 40.0%
!! TGTTTATCACAGTTGGTGGA+AGG + chr1.1:69389298-69389317 MS.gene072418:CDS 40.0%
ACTACTCGAACCATTGAGGA+TGG + chr1.1:69388097-69388116 MS.gene072418:CDS 45.0%
ATCCTCAATGGTTCGAGTAG+TGG - chr1.1:69388098-69388117 None:intergenic 45.0%
CAGTGCATGTCTTTCAGCAA+TGG + chr1.1:69387862-69387881 MS.gene072418:CDS 45.0%
CTCCCAAACTGTTGAAAGCA+CGG - chr1.1:69389374-69389393 None:intergenic 45.0%
CTCTACTTTCATCAGGGACA+AGG - chr1.1:69388165-69388184 None:intergenic 45.0%
CTTGTCCCTGATGAAAGTAG+AGG + chr1.1:69388163-69388182 MS.gene072418:CDS 45.0%
GAAAATGCACCACCACTTTC+AGG + chr1.1:69388786-69388805 MS.gene072418:CDS 45.0%
GAGAGTTTACGGACTCTCAA+TGG + chr1.1:69388129-69388148 MS.gene072418:CDS 45.0%
GTTTACGGACTCTCAATGGT+CGG + chr1.1:69388133-69388152 MS.gene072418:CDS 45.0%
TAAGGGATTGAGCATGTTGG+AGG + chr1.1:69388930-69388949 MS.gene072418:intron 45.0%
TGAGAGTCCGTAAACTCTCT+CGG - chr1.1:69388128-69388147 None:intergenic 45.0%
TGCCGTGCTTTCAACAGTTT+GGG + chr1.1:69389369-69389388 MS.gene072418:CDS 45.0%
TTCCACTACTCGAACCATTG+AGG + chr1.1:69388093-69388112 MS.gene072418:CDS 45.0%
TTGCCGTGCTTTCAACAGTT+TGG + chr1.1:69389368-69389387 MS.gene072418:CDS 45.0%
!! GAATTTTAAGGAAGCGGTGG+TGG + chr1.1:69388497-69388516 MS.gene072418:CDS 45.0%
ACTCGAACCATTGAGGATGG+TGG + chr1.1:69388100-69388119 MS.gene072418:CDS 50.0%
AGCACTCGGACTTGCCTAAT+AGG + chr1.1:69388026-69388045 MS.gene072418:CDS 50.0%
AGCATGCGGTCAAGCCTATT+AGG - chr1.1:69388043-69388062 None:intergenic 50.0%
AGTATCATGGCATCACCCTG+TGG - chr1.1:69388975-69388994 None:intergenic 50.0%
ATAGCTAAGGCAACACCACC+TGG + chr1.1:69387953-69387972 MS.gene072418:CDS 50.0%
GGGTGATGCCATGATACTGA+AGG + chr1.1:69388977-69388996 MS.gene072418:intron 50.0%
GTTGATGTTGGAGGTGGAAG+TGG + chr1.1:69388690-69388709 MS.gene072418:CDS 50.0%
TAGCAAGCAAACGCAGCATG+CGG - chr1.1:69388057-69388076 None:intergenic 50.0%
!! GATGGTGACATGAAAGCACC+AGG - chr1.1:69387974-69387993 None:intergenic 50.0%
ACCAGCATCAACGCAGCACT+CGG + chr1.1:69388012-69388031 MS.gene072418:CDS 55.0%
! GGTGGTGCCGAGAGAGTTTA+CGG + chr1.1:69388118-69388137 MS.gene072418:CDS 55.0%
!! GGTGACATGAAAGCACCAGG+TGG - chr1.1:69387971-69387990 None:intergenic 55.0%
CTCGGCACCACCATCCTCAA+TGG - chr1.1:69388110-69388129 None:intergenic 60.0%
! TCCGAGTGCTGCGTTGATGC+TGG - chr1.1:69388016-69388035 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 69387797 69389413 69387797 ID=MS.gene072418
chr1.1 mRNA 69387797 69389413 69387797 ID=MS.gene072418.t1;Parent=MS.gene072418
chr1.1 exon 69387797 69388239 69387797 ID=MS.gene072418.t1.exon1;Parent=MS.gene072418.t1
chr1.1 CDS 69387797 69388239 69387797 ID=cds.MS.gene072418.t1;Parent=MS.gene072418.t1
chr1.1 exon 69388494 69388807 69388494 ID=MS.gene072418.t1.exon2;Parent=MS.gene072418.t1
chr1.1 CDS 69388494 69388807 69388494 ID=cds.MS.gene072418.t1;Parent=MS.gene072418.t1
chr1.1 exon 69389157 69389413 69389157 ID=MS.gene072418.t1.exon3;Parent=MS.gene072418.t1
chr1.1 CDS 69389157 69389413 69389157 ID=cds.MS.gene072418.t1;Parent=MS.gene072418.t1
Gene Sequence

>MS.gene072418

ATGGGAAATTCCTACATTACCAAGGAGGATAACCAAATTAGTGCTACCTCAGAACAAACTGAAGACAGTGCATGTCTTTCAGCAATGGTACTTACCACTAATCTTGTTTATCCAGCAGTGTTAAATGCTGCTATTGATCTCAATTTATTTGAGATCATAGCTAAGGCAACACCACCTGGTGCTTTCATGTCACCATCTGAAATTGCTTCTAAATTACCAGCATCAACGCAGCACTCGGACTTGCCTAATAGGCTTGACCGCATGCTGCGTTTGCTTGCTAGTTATTCTGTTCTTACTTCCACTACTCGAACCATTGAGGATGGTGGTGCCGAGAGAGTTTACGGACTCTCAATGGTCGGAAAATACCTTGTCCCTGATGAAAGTAGAGGTTATTTGGCTTCATTTACTACATTTCTATGTTATCCTGCATTATTACAAGTTTGGTAAGTTATTTATTTCTCATTTCTCATACATATATAAAATCAAGGGATAGTGACTTGGATAAATAGTTTAATTAAATATTCACCTATTAAACATATGGAGGATATATAAGTTATAAAGGTAGCTTTATAAAATAAAATAAAAAGTTAATATAAATAATGTTAAAGAGAAAGTGATAGTTTAAGCTAATTGTAGCAATTAGGTATTTAATCTTACTTTTGTTCCAATTATGGTCTAAGCTTTAGTTTCTATCCAGGATGAATTTTAAGGAAGCGGTGGTGGATGAAGACATTGACTTGTTCAAGAACGTTCATGGAGTGACAAAGTATGAATTCATGGGAAAGGATAAAAAAATGAACCAAATTTTTAACAAATCAATGGTTGATGTATGTGCTACAGAGATGAAAAGAATGCTTGAAATATACACTGGATTTGAGGGAATATCAACATTAGTTGATGTTGGAGGTGGAAGTGGAAGAAATCTTGAATTGATAATATCCAAATATCCATTAATAAAGGGAATTAACTTTGATCTTCCCCAAGTTATTGAAAATGCACCACCACTTTCAGGTATAACATGATATATAATAATTTTTCTTCACATATATCATTATCAACAAGCCATCTTATACTAAAGTTTTACTTTGCATGTATATTATAATTAATAAAGTATATATACCACCAACTTTTGTTAAGGGATTGAGCATGTTGGAGGAGATATGTTTGCAAGTGTTCCACAGGGTGATGCCATGATACTGAAGGTAAGTATATAAATATTAATTAGTTAGATCTAACTGATTAATTAATTACTTGAAACTTTATAGATTAATTTATCTGATTTTTTTTAATTAAAGTGCTAACTACAACTTTTTGTTGACTTTGTTTACATAATTGTAGGCTGTATGCCATAATTGGTCAGATGAAAAATGCATAGAATTTTTAAGCAATTGTCACAAAGCTTTATCACCAAATGGAAAAGTGATTATTGTGGAGTTCATATTGCCAGAAGAACCAAACACAAGTGAAGAATCTAAGCTTGTTTCAACTCTTGACAATCTCATGTTTATCACAGTTGGTGGAAGGGAAGAGAAATTGAGCAAACTCTCTGGATTTTCCAAATTTCAAGTTGCTTGCCGTGCTTTCAACAGTTTGGGAGTGATGGAATTTTATAAATGA
Protein sequence

>MS.gene072418.t1

MGNSYITKEDNQISATSEQTEDSACLSAMVLTTNLVYPAVLNAAIDLNLFEIIAKATPPGAFMSPSEIASKLPASTQHSDLPNRLDRMLRLLASYSVLTSTTRTIEDGGAERVYGLSMVGKYLVPDESRGYLASFTTFLCYPALLQVWMNFKEAVVDEDIDLFKNVHGVTKYEFMGKDKKMNQIFNKSMVDVCATEMKRMLEIYTGFEGISTLVDVGGGSGRNLELIISKYPLIKGINFDLPQVIENAPPLSDEKCIEFLSNCHKALSPNGKVIIVEFILPEEPNTSEESKLVSTLDNLMFITVGGREEKLSKLSGFSKFQVACRAFNSLGVMEFYK