Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072442.t1 | XP_003626325.2 | 90.3 | 145 | 10 | 2 | 1 | 145 | 24 | 164 | 7.00E-68 | 266.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072442.t1 | G7KZQ0 | 90.3 | 145 | 10 | 2 | 1 | 145 | 24 | 164 | 5.1e-68 | 266.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049341 | MS.gene072442 | 0.801918 | 7.18E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene072442 | 0.804821 | 1.80E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene072442 | 0.837192 | 6.01E-57 | -1.69E-46 |
| MS.gene050438 | MS.gene072442 | 0.810998 | 8.72E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene072442 | 0.817091 | 3.95E-52 | -1.69E-46 |
| MS.gene050815 | MS.gene072442 | 0.811305 | 7.48E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene072442 | 0.82793 | 1.19E-54 | -1.69E-46 |
| MS.gene051720 | MS.gene072442 | 0.808188 | 3.50E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene072442 | 0.825179 | 5.40E-54 | -1.69E-46 |
| MS.gene052323 | MS.gene072442 | 0.806721 | 7.17E-50 | -1.69E-46 |
| MS.gene052613 | MS.gene072442 | 0.803851 | 2.86E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene072442 | 0.820031 | 8.50E-53 | -1.69E-46 |
| MS.gene052896 | MS.gene072442 | 0.800315 | 1.53E-48 | -1.69E-46 |
| MS.gene053221 | MS.gene072442 | 0.806344 | 8.61E-50 | -1.69E-46 |
| MS.gene055357 | MS.gene072442 | 0.804564 | 2.03E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene072442 | 0.802373 | 5.78E-49 | -1.69E-46 |
| MS.gene056366 | MS.gene072442 | 0.805725 | 1.16E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene072442 | 0.804257 | 2.36E-49 | -1.69E-46 |
| MS.gene058636 | MS.gene072442 | 0.814714 | 1.34E-51 | -1.69E-46 |
| MS.gene059937 | MS.gene072442 | 0.82447 | 7.93E-54 | -1.69E-46 |
| MS.gene060587 | MS.gene072442 | 0.817872 | 2.63E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072442.t1 | MTR_7g113900 | 90.345 | 145 | 10 | 2 | 1 | 145 | 24 | 164 | 3.34e-86 | 249 |
| MS.gene072442.t1 | MTR_2g024090 | 40.132 | 152 | 81 | 3 | 1 | 142 | 16 | 167 | 1.38e-29 | 106 |
| MS.gene072442.t1 | MTR_4g050050 | 35.625 | 160 | 86 | 2 | 1 | 143 | 18 | 177 | 1.74e-28 | 103 |
| MS.gene072442.t1 | MTR_4g050050 | 36.364 | 143 | 75 | 2 | 1 | 143 | 18 | 144 | 4.62e-27 | 99.4 |
| MS.gene072442.t1 | MTR_2g450100 | 42.982 | 114 | 62 | 1 | 33 | 143 | 63 | 176 | 1.42e-23 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072442.t1 | AT1G05070 | 41.060 | 151 | 70 | 5 | 8 | 143 | 22 | 168 | 1.23e-31 | 111 |
| MS.gene072442.t1 | AT2G32580 | 37.662 | 154 | 84 | 3 | 1 | 143 | 15 | 167 | 4.85e-30 | 107 |
| MS.gene072442.t1 | AT4G04360 | 41.935 | 155 | 76 | 4 | 1 | 143 | 16 | 168 | 1.45e-28 | 103 |
| MS.gene072442.t1 | AT2G24290 | 39.873 | 158 | 80 | 3 | 1 | 143 | 15 | 172 | 2.74e-28 | 102 |
| MS.gene072442.t1 | AT4G30996 | 50.515 | 97 | 48 | 0 | 45 | 141 | 73 | 169 | 1.41e-27 | 100 |
| MS.gene072442.t1 | AT2G32580 | 43.000 | 100 | 57 | 0 | 44 | 143 | 6 | 105 | 1.58e-24 | 91.7 |
Find 40 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAATATCCCGTTGCTTCTT+TGG | 0.212587 | 7.3:+3010025 | MS.gene072442:CDS |
| TGCATTTATTACTAATCTTT+TGG | 0.212992 | 7.3:-3010835 | None:intergenic |
| GAAGAACGAGCTCGTGATTA+TGG | 0.234749 | 7.3:+3010984 | MS.gene072442:CDS |
| TTCATTTCCTCCTTCATTGT+TGG | 0.237361 | 7.3:-3010738 | None:intergenic |
| AGCGCAGGTTGACGGCATTA+TGG | 0.273292 | 7.3:+3010961 | MS.gene072442:CDS |
| CTGTTCCTCACTTGCCTCAC+TGG | 0.367272 | 7.3:+3010004 | MS.gene072442:CDS |
| TGTTACCTTGTGTTCACAAT+TGG | 0.371180 | 7.3:+3009924 | MS.gene072442:CDS |
| GAATATCCCGTTGCTTCTTT+GGG | 0.376906 | 7.3:+3010026 | MS.gene072442:CDS |
| GCGCAGGTTGACGGCATTAT+GGG | 0.391190 | 7.3:+3010962 | MS.gene072442:CDS |
| TTACTATATGTACAGATTGT+GGG | 0.418392 | 7.3:+3010706 | MS.gene072442:intron |
| GAAGTAGAAAAGTGCAGCAT+AGG | 0.426789 | 7.3:+3010885 | MS.gene072442:CDS |
| CAGAGCTTGTTGAAGAGCGC+AGG | 0.429709 | 7.3:+3010946 | MS.gene072442:CDS |
| AAGAACGAGCTCGTGATTAT+GGG | 0.441049 | 7.3:+3010985 | MS.gene072442:CDS |
| GGTCTGTTCCAAAGTCTCAT+TGG | 0.443502 | 7.3:-3010814 | None:intergenic |
| AATTGTAGCCAATGAGACTT+TGG | 0.447499 | 7.3:+3010806 | MS.gene072442:CDS |
| GTGATTATGGGTGGAAGGAC+AGG | 0.457853 | 7.3:+3010997 | MS.gene072442:CDS |
| AACTTACCCAAAGAAGCAAC+GGG | 0.499220 | 7.3:-3010032 | None:intergenic |
| TGTTGAAGAGCGCAGGTTGA+CGG | 0.500440 | 7.3:+3010953 | MS.gene072442:CDS |
| AAGTAGAAAAGTGCAGCATA+GGG | 0.518644 | 7.3:+3010886 | MS.gene072442:CDS |
| TTCTTCAAACGCCAGCGAGA+TGG | 0.541960 | 7.3:-3009951 | None:intergenic |
| AGAAAAGTGCAGCATAGGGA+TGG | 0.556983 | 7.3:+3010890 | MS.gene072442:CDS |
| CAATCATGACATTGAGGACA+AGG | 0.561176 | 7.3:-3009978 | None:intergenic |
| CAATTGGACCACCATCTCGC+TGG | 0.563845 | 7.3:+3009940 | MS.gene072442:CDS |
| TGGAAACTTGTGAAGAAGCA+AGG | 0.573605 | 7.3:+3010910 | MS.gene072442:CDS |
| TTCAAACGCCAGCGAGATGG+TGG | 0.580007 | 7.3:-3009948 | None:intergenic |
| AGAAGCAAGGGAGAGAGCAG+AGG | 0.585238 | 7.3:+3010923 | MS.gene072442:CDS |
| GAGACATGATCCAACAATGA+AGG | 0.590741 | 7.3:+3010728 | MS.gene072442:CDS |
| AGCTCGTGATTATGGGTGGA+AGG | 0.596678 | 7.3:+3010992 | MS.gene072442:CDS |
| AAAGATTAGTAATAAATGCA+AGG | 0.596837 | 7.3:+3010838 | MS.gene072442:CDS |
| TCCTCTGATAGCATTGTCAG+TGG | 0.601328 | 7.3:-3010768 | None:intergenic |
| AAACTTACCCAAAGAAGCAA+CGG | 0.604283 | 7.3:-3010033 | None:intergenic |
| AACGAGCTCGTGATTATGGG+TGG | 0.612321 | 7.3:+3010988 | MS.gene072442:CDS |
| GGAAACTTGTGAAGAAGCAA+GGG | 0.615984 | 7.3:+3010911 | MS.gene072442:CDS |
| CAGTAACAATCATGACATTG+AGG | 0.634830 | 7.3:-3009984 | None:intergenic |
| TCCACTGACAATGCTATCAG+AGG | 0.642154 | 7.3:+3010767 | MS.gene072442:CDS |
| ATATTCCAGTGAGGCAAGTG+AGG | 0.642772 | 7.3:-3010009 | None:intergenic |
| ACATGATCCAACAATGAAGG+AGG | 0.647322 | 7.3:+3010731 | MS.gene072442:CDS |
| GTGGTCCAATTGTGAACACA+AGG | 0.658093 | 7.3:-3009929 | None:intergenic |
| AATGAAGGAGGAAATGAACA+AGG | 0.667487 | 7.3:+3010743 | MS.gene072442:CDS |
| AGCAACGGGATATTCCAGTG+AGG | 0.784097 | 7.3:-3010018 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATAAATTTTACTTAAAG+TGG | - | chr7.3:3010564-3010583 | None:intergenic | 10.0% |
| !! | AAAGATTAGTAATAAATGCA+AGG | + | chr7.3:3010838-3010857 | MS.gene072442:CDS | 20.0% |
| !! | AGAAATGCAAAACATTTAAA+TGG | - | chr7.3:3010475-3010494 | None:intergenic | 20.0% |
| !! | CAATTCAATTCATATGTATT+AGG | + | chr7.3:3010255-3010274 | MS.gene072442:intron | 20.0% |
| !! | TAGGTTTATATATTAATTCG+TGG | + | chr7.3:3010274-3010293 | MS.gene072442:intron | 20.0% |
| !! | TAGTAATTAGAAAAATTCAC+TGG | + | chr7.3:3010168-3010187 | MS.gene072442:intron | 20.0% |
| !!! | TGCATTTATTACTAATCTTT+TGG | - | chr7.3:3010838-3010857 | None:intergenic | 20.0% |
| ! | ATTGAATAACCAAAACATTC+TGG | - | chr7.3:3010239-3010258 | None:intergenic | 25.0% |
| ! | ATTTGTAGAGTAAGTGAAAA+AGG | - | chr7.3:3010060-3010079 | None:intergenic | 25.0% |
| ! | TTACTATATGTACAGATTGT+GGG | + | chr7.3:3010706-3010725 | MS.gene072442:intron | 25.0% |
| ! | TTTACTATATGTACAGATTG+TGG | + | chr7.3:3010705-3010724 | MS.gene072442:intron | 25.0% |
| ! | TTTGTAGAGTAAGTGAAAAA+GGG | - | chr7.3:3010059-3010078 | None:intergenic | 25.0% |
| !!! | CATCATTTTTCAAACACAAA+GGG | - | chr7.3:3010145-3010164 | None:intergenic | 25.0% |
| ACAAGTTTGATTAGATGTAC+AGG | + | chr7.3:3010108-3010127 | MS.gene072442:intron | 30.0% | |
| ACTCTACAAATTAACAGACT+TGG | + | chr7.3:3010069-3010088 | MS.gene072442:intron | 30.0% | |
| ATAATGTCCAAAAGACAACA+TGG | - | chr7.3:3010667-3010686 | None:intergenic | 30.0% | |
| CTAAAAATGAGAACAGAATG+AGG | - | chr7.3:3010624-3010643 | None:intergenic | 30.0% | |
| CTTTGTTCTGATTCTCAAAA+AGG | - | chr7.3:3010197-3010216 | None:intergenic | 30.0% | |
| TGATACAAAACATACATCGA+AGG | + | chr7.3:3010368-3010387 | MS.gene072442:intron | 30.0% | |
| ! | ATGTATGTTTTGTATCACAG+TGG | - | chr7.3:3010365-3010384 | None:intergenic | 30.0% |
| !! | GCATCATTTTTCAAACACAA+AGG | - | chr7.3:3010146-3010165 | None:intergenic | 30.0% |
| AAACTTACCCAAAGAAGCAA+CGG | - | chr7.3:3010036-3010055 | None:intergenic | 35.0% | |
| AATGAAGGAGGAAATGAACA+AGG | + | chr7.3:3010743-3010762 | MS.gene072442:CDS | 35.0% | |
| AATTGTAGCCAATGAGACTT+TGG | + | chr7.3:3010806-3010825 | MS.gene072442:CDS | 35.0% | |
| CAGTAACAATCATGACATTG+AGG | - | chr7.3:3009987-3010006 | None:intergenic | 35.0% | |
| TGTTACCTTGTGTTCACAAT+TGG | + | chr7.3:3009924-3009943 | MS.gene072442:CDS | 35.0% | |
| TTCATTTCCTCCTTCATTGT+TGG | - | chr7.3:3010741-3010760 | None:intergenic | 35.0% | |
| ! | AAGTAGAAAAGTGCAGCATA+GGG | + | chr7.3:3010886-3010905 | MS.gene072442:CDS | 35.0% |
| ! | TCTATCTAGCCAGAATGTTT+TGG | + | chr7.3:3010227-3010246 | MS.gene072442:intron | 35.0% |
| !! | CATAGCTAGCTTTGTTTATG+TGG | + | chr7.3:3010500-3010519 | MS.gene072442:intron | 35.0% |
| AACTTACCCAAAGAAGCAAC+GGG | - | chr7.3:3010035-3010054 | None:intergenic | 40.0% | |
| AAGAACGAGCTCGTGATTAT+GGG | + | chr7.3:3010985-3011004 | MS.gene072442:CDS | 40.0% | |
| ACATGATCCAACAATGAAGG+AGG | + | chr7.3:3010731-3010750 | MS.gene072442:CDS | 40.0% | |
| ATGTCCAAAAGACAACATGG+TGG | - | chr7.3:3010664-3010683 | None:intergenic | 40.0% | |
| CAATCATGACATTGAGGACA+AGG | - | chr7.3:3009981-3010000 | None:intergenic | 40.0% | |
| GAATATCCCGTTGCTTCTTT+GGG | + | chr7.3:3010026-3010045 | MS.gene072442:CDS | 40.0% | |
| GAGACATGATCCAACAATGA+AGG | + | chr7.3:3010728-3010747 | MS.gene072442:CDS | 40.0% | |
| GGAAACTTGTGAAGAAGCAA+GGG | + | chr7.3:3010911-3010930 | MS.gene072442:CDS | 40.0% | |
| TGGAAACTTGTGAAGAAGCA+AGG | + | chr7.3:3010910-3010929 | MS.gene072442:CDS | 40.0% | |
| ! | GAAGTAGAAAAGTGCAGCAT+AGG | + | chr7.3:3010885-3010904 | MS.gene072442:CDS | 40.0% |
| ! | TTCACCACCATGTTGTCTTT+TGG | + | chr7.3:3010657-3010676 | MS.gene072442:intron | 40.0% |
| ! | TTGTTTAGCTCAACACGGTA+CGG | + | chr7.3:3010398-3010417 | MS.gene072442:intron | 40.0% |
| !! | TTGCCTTGTTTAGCTCAACA+CGG | + | chr7.3:3010393-3010412 | MS.gene072442:intron | 40.0% |
| ATATTCCAGTGAGGCAAGTG+AGG | - | chr7.3:3010012-3010031 | None:intergenic | 45.0% | |
| GAAGAACGAGCTCGTGATTA+TGG | + | chr7.3:3010984-3011003 | MS.gene072442:CDS | 45.0% | |
| GGAATATCCCGTTGCTTCTT+TGG | + | chr7.3:3010025-3010044 | MS.gene072442:CDS | 45.0% | |
| GGTCTGTTCCAAAGTCTCAT+TGG | - | chr7.3:3010817-3010836 | None:intergenic | 45.0% | |
| GTACCGTGTTGAGCTAAACA+AGG | - | chr7.3:3010399-3010418 | None:intergenic | 45.0% | |
| GTGGTCCAATTGTGAACACA+AGG | - | chr7.3:3009932-3009951 | None:intergenic | 45.0% | |
| TCCACTGACAATGCTATCAG+AGG | + | chr7.3:3010767-3010786 | MS.gene072442:CDS | 45.0% | |
| TCCTCTGATAGCATTGTCAG+TGG | - | chr7.3:3010771-3010790 | None:intergenic | 45.0% | |
| ! | AGAAAAGTGCAGCATAGGGA+TGG | + | chr7.3:3010890-3010909 | MS.gene072442:CDS | 45.0% |
| ! | TGTTTAGCTCAACACGGTAC+GGG | + | chr7.3:3010399-3010418 | MS.gene072442:intron | 45.0% |
| AACGAGCTCGTGATTATGGG+TGG | + | chr7.3:3010988-3011007 | MS.gene072442:CDS | 50.0% | |
| AGCAACGGGATATTCCAGTG+AGG | - | chr7.3:3010021-3010040 | None:intergenic | 50.0% | |
| AGCTCGTGATTATGGGTGGA+AGG | + | chr7.3:3010992-3011011 | MS.gene072442:CDS | 50.0% | |
| GTGATTATGGGTGGAAGGAC+AGG | + | chr7.3:3010997-3011016 | MS.gene072442:CDS | 50.0% | |
| TTCTTCAAACGCCAGCGAGA+TGG | - | chr7.3:3009954-3009973 | None:intergenic | 50.0% | |
| ! | GTTTAGCTCAACACGGTACG+GGG | + | chr7.3:3010400-3010419 | MS.gene072442:intron | 50.0% |
| ! | TGTTGAAGAGCGCAGGTTGA+CGG | + | chr7.3:3010953-3010972 | MS.gene072442:CDS | 50.0% |
| AGAAGCAAGGGAGAGAGCAG+AGG | + | chr7.3:3010923-3010942 | MS.gene072442:CDS | 55.0% | |
| CAATTGGACCACCATCTCGC+TGG | + | chr7.3:3009940-3009959 | MS.gene072442:CDS | 55.0% | |
| CTGTTCCTCACTTGCCTCAC+TGG | + | chr7.3:3010004-3010023 | MS.gene072442:CDS | 55.0% | |
| TTCAAACGCCAGCGAGATGG+TGG | - | chr7.3:3009951-3009970 | None:intergenic | 55.0% | |
| ! | AGCGCAGGTTGACGGCATTA+TGG | + | chr7.3:3010961-3010980 | MS.gene072442:CDS | 55.0% |
| ! | GCGCAGGTTGACGGCATTAT+GGG | + | chr7.3:3010962-3010981 | MS.gene072442:CDS | 55.0% |
| !! | CAGAGCTTGTTGAAGAGCGC+AGG | + | chr7.3:3010946-3010965 | MS.gene072442:CDS | 55.0% |
| GCTCAACACGGTACGGGGAT+TGG | + | chr7.3:3010405-3010424 | MS.gene072442:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 3009915 | 3011025 | 3009915 | ID=MS.gene072442 |
| chr7.3 | mRNA | 3009915 | 3011025 | 3009915 | ID=MS.gene072442.t1;Parent=MS.gene072442 |
| chr7.3 | exon | 3009915 | 3010047 | 3009915 | ID=MS.gene072442.t1.exon1;Parent=MS.gene072442.t1 |
| chr7.3 | CDS | 3009915 | 3010047 | 3009915 | ID=cds.MS.gene072442.t1;Parent=MS.gene072442.t1 |
| chr7.3 | exon | 3010721 | 3011025 | 3010721 | ID=MS.gene072442.t1.exon2;Parent=MS.gene072442.t1 |
| chr7.3 | CDS | 3010721 | 3011025 | 3010721 | ID=cds.MS.gene072442.t1;Parent=MS.gene072442.t1 |
| Gene Sequence |
| Protein sequence |
