AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072716


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072716.t1 MTR_7g109250 96.942 327 10 0 1 327 1 327 0.0 661
MS.gene072716.t1 MTR_7g109250 96.942 327 10 0 1 327 61 387 0.0 658
MS.gene072716.t1 MTR_7g108868 51.412 177 26 5 62 238 11 127 1.36e-46 156
MS.gene072716.t1 MTR_8g058593 51.412 177 26 5 62 238 11 127 1.36e-46 156
MS.gene072716.t1 MTR_8g058607 50.000 176 28 4 62 237 24 139 1.16e-45 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072716.t1 AT1G01350 69.375 320 95 2 8 327 20 336 3.31e-163 459
MS.gene072716.t1 AT5G06420 68.125 320 96 2 8 327 58 371 3.17e-161 455
MS.gene072716.t1 AT5G06420 68.125 320 96 2 8 327 58 371 3.17e-161 455
MS.gene072716.t1 AT4G01023 64.407 59 21 0 258 316 76 134 2.53e-26 104
MS.gene072716.t1 AT4G01023 65.079 63 21 1 254 316 92 153 5.86e-26 104
MS.gene072716.t1 AT4G01023 64.407 59 21 0 258 316 95 153 8.26e-26 103

Find 66 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATATTCTTCCTATTAAC+AGG 0.250339 7.3:+5613040 None:intergenic
TCTTCAGAAAACCTGTTAAT+AGG 0.277151 7.3:-5613051 MS.gene072716:CDS
TTGTAATCCTTGCATATATC+TGG 0.290645 7.3:+5612533 None:intergenic
GTGGGTCACACGGTCCTCTC+AGG 0.313412 7.3:-5612598 MS.gene072716:CDS
GATCGAGGTGATTACAAATC+TGG 0.331028 7.3:-5612464 MS.gene072716:CDS
TGCAAGGATTACAAGGAAAC+TGG 0.409524 7.3:-5612524 MS.gene072716:CDS
AAATGAAAGCTGGGTTTCTC+AGG 0.415855 7.3:+5612863 None:intergenic
CATTTGTCGAAACCCATTTG+TGG 0.418726 7.3:-5612303 MS.gene072716:CDS
CTTGCTTAAGAGCTCTTTCA+CGG 0.431115 7.3:+5612747 None:intergenic
CTGTGAGCATTGTGCATTGA+AGG 0.436410 7.3:-5612249 MS.gene072716:intron
TAGCAAAGCAACAGCAACTT+TGG 0.436558 7.3:-5612807 MS.gene072716:CDS
GTTGAGACAGGATCCACAAA+TGG 0.441292 7.3:+5612290 None:intergenic
AGAAGACTCGAAATGAAAGC+TGG 0.441893 7.3:+5612853 None:intergenic
ACACGGTCCTCTCAGGGCTT+CGG 0.450938 7.3:-5612591 MS.gene072716:CDS
TGCTTACACTTGGTTGAGAC+AGG 0.460245 7.3:+5612278 None:intergenic
GTCTGCAATCAGCCTACGCT+TGG 0.462383 7.3:-5612081 MS.gene072716:CDS
CAATAATTATACAGATCATA+AGG 0.469416 7.3:-5612663 MS.gene072716:CDS
GGTGGCAGATGGAGAAAGAA+TGG 0.469953 7.3:-5612442 MS.gene072716:CDS
TGCAGGTGAGGATGCGGAAG+AGG 0.471533 7.3:-5612384 MS.gene072716:CDS
CGAGGATGTAAAATTATACA+AGG 0.486690 7.3:-5612690 MS.gene072716:CDS
ACATACCTTGTTCAGTCGGC+TGG 0.489348 7.3:+5613189 None:intergenic
GCAAACAATTGCCAGTGAAA+AGG 0.499122 7.3:-5612624 MS.gene072716:CDS
CGACAAATGAAACACGCAAA+TGG 0.499537 7.3:+5612317 None:intergenic
GAAAGCAAGGAAGATGAGAT+TGG 0.507679 7.3:-5612408 MS.gene072716:CDS
TGCAGAGCTCTTCGAAGAGC+CGG 0.511210 7.3:+5612907 None:intergenic
AATTATACAGATCATAAGGC+TGG 0.520545 7.3:-5612659 MS.gene072716:CDS
ATCGAGGTGATTACAAATCT+GGG 0.520829 7.3:-5612463 MS.gene072716:CDS
GAGCTCTTTCACGGATGGCA+CGG 0.524231 7.3:+5612756 None:intergenic
TTGAGACAGGATCCACAAAT+GGG 0.525917 7.3:+5612291 None:intergenic
AGATATATGCAAGGATTACA+AGG 0.530308 7.3:-5612531 MS.gene072716:CDS
TGGGTCACACGGTCCTCTCA+GGG 0.532282 7.3:-5612597 MS.gene072716:CDS
CTTAAGAGCTCTTTCACGGA+TGG 0.538525 7.3:+5612751 None:intergenic
AGTCTCAAGGGAAAGAGTAC+TGG 0.538575 7.3:-5612716 MS.gene072716:CDS
GAAAACCAGCCGACTGAACA+AGG 0.548242 7.3:-5613194 MS.gene072716:intron
GTGGCAGATGGAGAAAGAAT+GGG 0.553122 7.3:-5612441 MS.gene072716:CDS
TTACAAATCTGGGTGGCAGA+TGG 0.553483 7.3:-5612453 MS.gene072716:CDS
GAAGACTCGAAATGAAAGCT+GGG 0.554193 7.3:+5612854 None:intergenic
AGCTCTTTCACGGATGGCAC+GGG 0.560125 7.3:+5612757 None:intergenic
GAAACTGGTTACTGTGGGTA+TGG 0.560524 7.3:-5612509 MS.gene072716:CDS
TATTGAGAATGAAGATAATG+AGG 0.560776 7.3:-5613008 MS.gene072716:CDS
TCATGAGATACGAAAGAAGA+TGG 0.563924 7.3:-5612043 MS.gene072716:CDS
ATAGGAAGAATATGAGGAAG+CGG 0.564756 7.3:-5613033 MS.gene072716:CDS
GGTGAGGATGCGGAAGAGGA+AGG 0.573050 7.3:-5612380 MS.gene072716:CDS
CTGTTAATAGGAAGAATATG+AGG 0.583376 7.3:-5613039 MS.gene072716:CDS
GGGATGAAGCTGAGAAAGCA+AGG 0.584805 7.3:-5612421 MS.gene072716:CDS
TACAAGGAAACTGGTTACTG+TGG 0.587264 7.3:-5612515 MS.gene072716:CDS
ACAATTGCCAGTGAAAAGGC+TGG 0.588175 7.3:-5612620 MS.gene072716:CDS
TTATACATACCTTGTTCAGT+CGG 0.588673 7.3:+5613185 None:intergenic
GATGAGATTGGCTGCAGGTG+AGG 0.590274 7.3:-5612396 MS.gene072716:CDS
GAAAAGGCTGGTGGGTCACA+CGG 0.600903 7.3:-5612608 MS.gene072716:CDS
AGGAAGATGAGATTGGCTGC+AGG 0.603143 7.3:-5612401 MS.gene072716:CDS
ATTGGCTGCAGGTGAGGATG+CGG 0.603342 7.3:-5612390 MS.gene072716:CDS
ATTGCCAGTGAAAAGGCTGG+TGG 0.607353 7.3:-5612617 MS.gene072716:CDS
GATACGAAAGAAGATGGCTG+AGG 0.623889 7.3:-5612037 MS.gene072716:CDS
TTGCCAGTGAAAAGGCTGGT+GGG 0.630138 7.3:-5612616 MS.gene072716:CDS
CAAGCGACTGAGAGTCTCAA+GGG 0.630489 7.3:-5612728 MS.gene072716:CDS
ACAGAAGTAGTGCTTACACT+TGG 0.630927 7.3:+5612268 None:intergenic
GCAAGCGACTGAGAGTCTCA+AGG 0.636269 7.3:-5612729 MS.gene072716:CDS
GGGAAAGAGTACTGGTTCCG+AGG 0.640397 7.3:-5612708 MS.gene072716:CDS
ACAAGGAAACTGGTTACTGT+GGG 0.647981 7.3:-5612514 MS.gene072716:CDS
TGTAAGTTTATGCATGATCG+AGG 0.650874 7.3:-5612479 MS.gene072716:CDS
TTATCAGCCAGATATATGCA+AGG 0.656472 7.3:-5612540 MS.gene072716:CDS
AGAGCTCTTCGAAGAGCCGG+TGG 0.686462 7.3:+5612910 None:intergenic
GAGGATGTAAAATTATACAA+GGG 0.688297 7.3:-5612689 MS.gene072716:CDS
ATGTGAGCCGAAGCCCTGAG+AGG 0.689295 7.3:+5612584 None:intergenic
GAGGTGATTACAAATCTGGG+TGG 0.758440 7.3:-5612460 MS.gene072716:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAAAAAGGTTAATGA+TGG + chr7.3:5612150-5612169 None:intergenic 20.0%
!! CAATAATTATACAGATCATA+AGG - chr7.3:5612587-5612606 MS.gene072716:CDS 20.0%
! AGAAGTTAGGCATAATTAAA+AGG + chr7.3:5613105-5613124 None:intergenic 25.0%
! GAGGATGTAAAATTATACAA+GGG - chr7.3:5612561-5612580 MS.gene072716:CDS 25.0%
! TATTGAGAATGAAGATAATG+AGG - chr7.3:5612242-5612261 MS.gene072716:intron 25.0%
!! CTTGTATAATTTTACATCCT+CGG + chr7.3:5612562-5612581 None:intergenic 25.0%
!!! ACTTATTTGCTTTGAAGTTA+AGG + chr7.3:5613032-5613051 None:intergenic 25.0%
!!! CTTATTTGCTTTGAAGTTAA+GGG + chr7.3:5613031-5613050 None:intergenic 25.0%
AAGTAAAATGAGCAGAAGTT+AGG + chr7.3:5613118-5613137 None:intergenic 30.0%
AATTATACAGATCATAAGGC+TGG - chr7.3:5612591-5612610 MS.gene072716:CDS 30.0%
ACAAACGCGTAGAAAAAAAA+AGG + chr7.3:5612160-5612179 None:intergenic 30.0%
AGATATATGCAAGGATTACA+AGG - chr7.3:5612719-5612738 MS.gene072716:CDS 30.0%
CGAGGATGTAAAATTATACA+AGG - chr7.3:5612560-5612579 MS.gene072716:CDS 30.0%
CTCATATTCTTCCTATTAAC+AGG + chr7.3:5612213-5612232 None:intergenic 30.0%
CTGTTAATAGGAAGAATATG+AGG - chr7.3:5612211-5612230 MS.gene072716:intron 30.0%
GATGGTACAAAAAAATGTAG+AGG + chr7.3:5612132-5612151 None:intergenic 30.0%
TCTTCAGAAAACCTGTTAAT+AGG - chr7.3:5612199-5612218 MS.gene072716:intron 30.0%
TGTTCAGAAAAAGAACATGA+AGG - chr7.3:5612296-5612315 MS.gene072716:CDS 30.0%
TTATACATACCTTGTTCAGT+CGG + chr7.3:5612068-5612087 None:intergenic 30.0%
TTGTAATCCTTGCATATATC+TGG + chr7.3:5612720-5612739 None:intergenic 30.0%
! AATAAGTTGTTCTTTTCCAC+CGG - chr7.3:5612324-5612343 MS.gene072716:CDS 30.0%
ACATTAAAAATGCCAAGCGT+AGG + chr7.3:5613184-5613203 None:intergenic 35.0%
ATAGGAAGAATATGAGGAAG+CGG - chr7.3:5612217-5612236 MS.gene072716:intron 35.0%
ATCGAGGTGATTACAAATCT+GGG - chr7.3:5612787-5612806 MS.gene072716:CDS 35.0%
TCATGAGATACGAAAGAAGA+TGG - chr7.3:5613207-5613226 MS.gene072716:CDS 35.0%
TGTAAGTTTATGCATGATCG+AGG - chr7.3:5612771-5612790 MS.gene072716:CDS 35.0%
TTATCAGCCAGATATATGCA+AGG - chr7.3:5612710-5612729 MS.gene072716:CDS 35.0%
! TACGCTTGGCATTTTTAATG+TGG - chr7.3:5613183-5613202 MS.gene072716:intron 35.0%
AAATGAAAGCTGGGTTTCTC+AGG + chr7.3:5612390-5612409 None:intergenic 40.0%
ACAAGGAAACTGGTTACTGT+GGG - chr7.3:5612736-5612755 MS.gene072716:CDS 40.0%
ACAGAAGTAGTGCTTACACT+TGG + chr7.3:5612985-5613004 None:intergenic 40.0%
AGAAGACTCGAAATGAAAGC+TGG + chr7.3:5612400-5612419 None:intergenic 40.0%
CATTTGTCGAAACCCATTTG+TGG - chr7.3:5612947-5612966 MS.gene072716:CDS 40.0%
CGACAAATGAAACACGCAAA+TGG + chr7.3:5612936-5612955 None:intergenic 40.0%
CTTGCTTAAGAGCTCTTTCA+CGG + chr7.3:5612506-5612525 None:intergenic 40.0%
GAAAGCAAGGAAGATGAGAT+TGG - chr7.3:5612842-5612861 MS.gene072716:CDS 40.0%
GAAGACTCGAAATGAAAGCT+GGG + chr7.3:5612399-5612418 None:intergenic 40.0%
GATCGAGGTGATTACAAATC+TGG - chr7.3:5612786-5612805 MS.gene072716:CDS 40.0%
GCAAACAATTGCCAGTGAAA+AGG - chr7.3:5612626-5612645 MS.gene072716:CDS 40.0%
TACAAGGAAACTGGTTACTG+TGG - chr7.3:5612735-5612754 MS.gene072716:CDS 40.0%
TAGCAAAGCAACAGCAACTT+TGG - chr7.3:5612443-5612462 MS.gene072716:CDS 40.0%
TGCAAGGATTACAAGGAAAC+TGG - chr7.3:5612726-5612745 MS.gene072716:CDS 40.0%
TTGAGACAGGATCCACAAAT+GGG + chr7.3:5612962-5612981 None:intergenic 40.0%
ACAATTGCCAGTGAAAAGGC+TGG - chr7.3:5612630-5612649 MS.gene072716:CDS 45.0%
CTTAAGAGCTCTTTCACGGA+TGG + chr7.3:5612502-5612521 None:intergenic 45.0%
GAAACTGGTTACTGTGGGTA+TGG - chr7.3:5612741-5612760 MS.gene072716:CDS 45.0%
GAGGTGATTACAAATCTGGG+TGG - chr7.3:5612790-5612809 MS.gene072716:CDS 45.0%
GATACGAAAGAAGATGGCTG+AGG - chr7.3:5613213-5613232 MS.gene072716:CDS 45.0%
GTGGCAGATGGAGAAAGAAT+GGG - chr7.3:5612809-5612828 MS.gene072716:CDS 45.0%
GTTGAGACAGGATCCACAAA+TGG + chr7.3:5612963-5612982 None:intergenic 45.0%
TGCTTACACTTGGTTGAGAC+AGG + chr7.3:5612975-5612994 None:intergenic 45.0%
TTACAAATCTGGGTGGCAGA+TGG - chr7.3:5612797-5612816 MS.gene072716:CDS 45.0%
! AGTCTCAAGGGAAAGAGTAC+TGG - chr7.3:5612534-5612553 MS.gene072716:CDS 45.0%
! CTGTGAGCATTGTGCATTGA+AGG - chr7.3:5613001-5613020 MS.gene072716:CDS 45.0%
ACATACCTTGTTCAGTCGGC+TGG + chr7.3:5612064-5612083 None:intergenic 50.0%
ATTGCCAGTGAAAAGGCTGG+TGG - chr7.3:5612633-5612652 MS.gene072716:CDS 50.0%
CAAGCGACTGAGAGTCTCAA+GGG - chr7.3:5612522-5612541 MS.gene072716:CDS 50.0%
GAAAACCAGCCGACTGAACA+AGG - chr7.3:5612056-5612075 MS.gene072716:CDS 50.0%
GGGATGAAGCTGAGAAAGCA+AGG - chr7.3:5612829-5612848 MS.gene072716:CDS 50.0%
GGTGGCAGATGGAGAAAGAA+TGG - chr7.3:5612808-5612827 MS.gene072716:CDS 50.0%
TTGCCAGTGAAAAGGCTGGT+GGG - chr7.3:5612634-5612653 MS.gene072716:CDS 50.0%
! AGGAAGATGAGATTGGCTGC+AGG - chr7.3:5612849-5612868 MS.gene072716:CDS 50.0%
!!! CGGCTGGTTTTCAGCAGATT+TGG + chr7.3:5612048-5612067 None:intergenic 50.0%
AGCTCTTTCACGGATGGCAC+GGG + chr7.3:5612496-5612515 None:intergenic 55.0%
ATTGGCTGCAGGTGAGGATG+CGG - chr7.3:5612860-5612879 MS.gene072716:CDS 55.0%
GAGCTCTTTCACGGATGGCA+CGG + chr7.3:5612497-5612516 None:intergenic 55.0%
GCAAGCGACTGAGAGTCTCA+AGG - chr7.3:5612521-5612540 MS.gene072716:CDS 55.0%
GTCTGCAATCAGCCTACGCT+TGG - chr7.3:5613169-5613188 MS.gene072716:intron 55.0%
TGCAGAGCTCTTCGAAGAGC+CGG + chr7.3:5612346-5612365 None:intergenic 55.0%
! GAAAAGGCTGGTGGGTCACA+CGG - chr7.3:5612642-5612661 MS.gene072716:CDS 55.0%
! GATGAGATTGGCTGCAGGTG+AGG - chr7.3:5612854-5612873 MS.gene072716:CDS 55.0%
! TGACCCACCAGCCTTTTCAC+TGG + chr7.3:5612640-5612659 None:intergenic 55.0%
!! GGGAAAGAGTACTGGTTCCG+AGG - chr7.3:5612542-5612561 MS.gene072716:CDS 55.0%
ACACGGTCCTCTCAGGGCTT+CGG - chr7.3:5612659-5612678 MS.gene072716:CDS 60.0%
AGAGCTCTTCGAAGAGCCGG+TGG + chr7.3:5612343-5612362 None:intergenic 60.0%
ATGTGAGCCGAAGCCCTGAG+AGG + chr7.3:5612669-5612688 None:intergenic 60.0%
GGTGAGGATGCGGAAGAGGA+AGG - chr7.3:5612870-5612889 MS.gene072716:CDS 60.0%
TGCAGGTGAGGATGCGGAAG+AGG - chr7.3:5612866-5612885 MS.gene072716:CDS 60.0%
TGGGTCACACGGTCCTCTCA+GGG - chr7.3:5612653-5612672 MS.gene072716:CDS 60.0%
GTGGGTCACACGGTCCTCTC+AGG - chr7.3:5612652-5612671 MS.gene072716:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 5612029 5613243 5612029 ID=MS.gene072716
chr7.3 mRNA 5612029 5613243 5612029 ID=MS.gene072716.t1;Parent=MS.gene072716
chr7.3 exon 5613195 5613243 5613195 ID=MS.gene072716.t1.exon1;Parent=MS.gene072716.t1
chr7.3 CDS 5613195 5613243 5613195 ID=cds.MS.gene072716.t1;Parent=MS.gene072716.t1
chr7.3 exon 5612250 5613082 5612250 ID=MS.gene072716.t1.exon2;Parent=MS.gene072716.t1
chr7.3 CDS 5612250 5613082 5612250 ID=cds.MS.gene072716.t1;Parent=MS.gene072716.t1
chr7.3 exon 5612029 5612130 5612029 ID=MS.gene072716.t1.exon3;Parent=MS.gene072716.t1
chr7.3 CDS 5612029 5612130 5612029 ID=cds.MS.gene072716.t1;Parent=MS.gene072716.t1
Gene Sequence

>MS.gene072716

ATGGAAAATTCTGAGGCCAAATCTGCTGAAAACCAGCCGACTGAACAAGGTATGTATAATATTTTGCATTATTAGACTCACAATCTTGTTATCTTATGCACCTCTACATTTTTTTGTACCATCATTAACCTTTTTTTTTCTACGCGTTTGTTTATCGTCAGTGTGTAGTTTCTTCAGAAAACCTGTTAATAGGAAGAATATGAGGAAGCGGACTATTGAGAATGAAGATAATGAGGATGACTCGAACAATGAAGAGTCTTTGATGCATGTTCAGAAAAAGAACATGAAGGCTGATAATAAGTTGTTCTTTTCCACCGGCTCTTCGAAGAGCTCTGCATCTGCTAAACTGAATGAAGAACCTGAGAAACCCAGCTTTCATTTCGAGTCTTCTAAAGAGATTCAAGTTCAACATGATAGCAAAGCAACAGCAACTTTGGAGACTGAGACTGATTTTTCAAGAGATGCCCGTGCCATCCGTGAAAGAGCTCTTAAGCAAGCGACTGAGAGTCTCAAGGGAAAGAGTACTGGTTCCGAGGATGTAAAATTATACAAGGGAATCAATAATTATACAGATCATAAGGCTGGATTTCGTAGAGAGCAAACAATTGCCAGTGAAAAGGCTGGTGGGTCACACGGTCCTCTCAGGGCTTCGGCTCACATAAGAGTTTCAGCAAGATTTGATTATCAGCCAGATATATGCAAGGATTACAAGGAAACTGGTTACTGTGGGTATGGTGATTCGTGTAAGTTTATGCATGATCGAGGTGATTACAAATCTGGGTGGCAGATGGAGAAAGAATGGGATGAAGCTGAGAAAGCAAGGAAGATGAGATTGGCTGCAGGTGAGGATGCGGAAGAGGAAGGTGCTAGTTTGAATGATGAAGATGATGATGAAGACGTATTACCATTTGCGTGTTTCATTTGTCGAAACCCATTTGTGGATCCTGTCTCAACCAAGTGTAAGCACTACTTCTGTGAGCATTGTGCATTGAAGGTACACCCTTAACTTCAAAGCAAATAAGTTTTAATTTATAACAGTTTCAAGTTATTTAGTAATGCTTAATTTCTTTTTTCCTTTTAATTATGCCTAACTTCTGCTCATTTTACTTGCAGCATCATGCGAAGAATAAGAAGTGTTTTGTCTGCAATCAGCCTACGCTTGGCATTTTTAATGTGGCTCATGAGATACGAAAGAAGATGGCTGAGGATAAATAG

Protein sequence

>MS.gene072716.t1

MENSEAKSAENQPTEQVCSFFRKPVNRKNMRKRTIENEDNEDDSNNEESLMHVQKKNMKADNKLFFSTGSSKSSASAKLNEEPEKPSFHFESSKEIQVQHDSKATATLETETDFSRDARAIRERALKQATESLKGKSTGSEDVKLYKGINNYTDHKAGFRREQTIASEKAGGSHGPLRASAHIRVSARFDYQPDICKDYKETGYCGYGDSCKFMHDRGDYKSGWQMEKEWDEAEKARKMRLAAGEDAEEEGASLNDEDDDEDVLPFACFICRNPFVDPVSTKCKHYFCEHCALKHHAKNKKCFVCNQPTLGIFNVAHEIRKKMAEDK