AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072808

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072808.t1 MTR_5g010680 95.342 365 11 2 1 365 1 359 0.0 709
MS.gene072808.t1 MTR_0020s0020 45.745 376 119 14 5 353 7 324 2.86e-81 252
MS.gene072808.t1 MTR_1g100777 45.773 343 117 11 6 347 5 279 3.48e-74 232
MS.gene072808.t1 MTR_3g101870 46.087 230 113 4 1 219 1 230 5.97e-60 197
MS.gene072808.t1 MTR_5g017470 45.490 255 112 7 17 260 2 240 1.58e-59 194
MS.gene072808.t1 MTR_5g089170 44.248 226 101 4 14 239 15 215 7.67e-58 189
MS.gene072808.t1 MTR_7g091370 44.724 199 75 3 18 216 16 179 4.47e-48 162
MS.gene072808.t1 MTR_5g029680 72.000 100 26 1 22 121 30 127 8.65e-46 164
MS.gene072808.t1 MTR_6g043060 60.952 105 41 0 24 128 22 126 9.28e-43 151
MS.gene072808.t1 MTR_7g095680 65.957 94 32 0 22 115 47 140 4.52e-42 150
MS.gene072808.t1 MTR_1g071360 67.021 94 31 0 22 115 30 123 7.23e-42 149
MS.gene072808.t1 MTR_1g071360 67.021 94 31 0 22 115 27 120 1.30e-41 149
MS.gene072808.t1 MTR_8g087540 64.356 101 35 1 15 115 4 103 1.62e-41 150
MS.gene072808.t1 MTR_4g077970 63.265 98 36 0 24 121 12 109 2.27e-40 149
MS.gene072808.t1 MTR_4g088900 37.624 202 89 3 21 218 30 198 3.20e-40 141
MS.gene072808.t1 MTR_8g083100 60.577 104 41 0 25 128 23 126 6.51e-40 147
MS.gene072808.t1 MTR_1g061170 62.245 98 37 0 18 115 43 140 1.74e-39 145
MS.gene072808.t1 MTR_2g100680 57.944 107 45 0 22 128 29 135 1.51e-38 143
MS.gene072808.t1 MTR_3g104550 62.500 96 36 0 22 117 44 139 3.55e-38 140
MS.gene072808.t1 MTR_1g102860 63.830 94 34 0 22 115 38 131 1.56e-37 138
MS.gene072808.t1 MTR_2g100670 52.137 117 56 0 12 128 21 137 3.58e-37 136
MS.gene072808.t1 MTR_6g086805 59.184 98 40 0 18 115 5 102 5.03e-37 137
MS.gene072808.t1 MTR_2g017890 51.240 121 58 1 11 131 3 122 1.79e-36 136
MS.gene072808.t1 MTR_2g100670 52.137 117 56 0 12 128 129 245 3.86e-36 136
MS.gene072808.t1 MTR_8g105780 59.140 93 37 1 24 115 22 114 3.42e-32 124
MS.gene072808.t1 MTR_4g045757 54.054 74 32 2 11 83 2 74 1.69e-19 82.4
MS.gene072808.t1 MTR_5g047190 42.857 91 45 2 32 115 1 91 6.98e-19 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072808.t1 AT4G11660 49.573 351 134 7 16 355 51 369 3.28e-90 276
MS.gene072808.t1 AT5G62020 46.302 311 116 8 1 305 1 266 9.38e-82 251
MS.gene072808.t1 AT4G36990 50.242 207 86 3 12 218 2 191 1.12e-61 199
MS.gene072808.t1 AT1G46264 37.248 298 172 5 19 306 29 321 4.41e-59 195
MS.gene072808.t1 AT2G41690 40.930 215 110 2 4 218 20 217 1.47e-48 164
MS.gene072808.t1 AT1G32330 65.289 121 38 2 1 121 16 132 6.56e-47 166
MS.gene072808.t1 AT1G32330 65.289 121 38 2 1 121 16 132 6.82e-47 166
MS.gene072808.t1 AT4G17750 61.667 120 46 0 2 121 30 149 1.34e-46 166
MS.gene072808.t1 AT3G02990 66.355 107 34 2 9 115 10 114 8.90e-43 155
MS.gene072808.t1 AT3G22830 67.347 98 32 0 18 115 55 152 6.48e-42 151
MS.gene072808.t1 AT4G13980 60.000 110 38 1 25 128 24 133 2.94e-40 148
MS.gene072808.t1 AT5G16820 67.708 96 30 1 20 115 24 118 7.49e-40 147
MS.gene072808.t1 AT5G16820 67.708 96 30 1 20 115 24 118 7.49e-40 147
MS.gene072808.t1 AT5G43840 63.830 94 34 0 22 115 17 110 2.67e-39 141
MS.gene072808.t1 AT2G26150 61.702 94 36 0 22 115 42 135 4.91e-39 142
MS.gene072808.t1 AT2G26150 61.702 94 36 0 22 115 42 135 4.91e-39 142
MS.gene072808.t1 AT3G63350 58.763 97 40 0 18 114 22 118 1.25e-38 139
MS.gene072808.t1 AT5G03720 64.211 95 33 1 21 115 53 146 5.01e-38 141
MS.gene072808.t1 AT5G03720 64.211 95 33 1 21 115 53 146 5.01e-38 141
MS.gene072808.t1 AT3G51910 60.215 93 37 0 22 114 27 119 1.07e-37 137
MS.gene072808.t1 AT5G54070 60.638 94 37 0 21 114 68 161 1.07e-37 138
MS.gene072808.t1 AT3G24520 65.217 92 32 0 24 115 17 108 1.14e-36 135
MS.gene072808.t1 AT4G18880 58.824 119 46 2 17 135 9 124 3.02e-35 133
MS.gene072808.t1 AT5G45710 58.621 116 44 3 20 135 10 121 1.23e-34 130
MS.gene072808.t1 AT5G45710 58.621 116 44 3 20 135 10 121 1.23e-34 130
MS.gene072808.t1 AT1G67970 60.215 93 36 1 24 115 19 111 9.89e-34 129
MS.gene072808.t1 AT4G18870 45.263 95 49 1 20 111 139 233 1.07e-22 97.1
MS.gene072808.t1 AT4G18870 47.191 89 42 3 25 111 14 99 3.48e-20 90.1
MS.gene072808.t1 AT1G77570 39.326 89 49 3 25 109 27 114 2.99e-13 67.0

Find 91 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATGTTCCTAAGCTGTTT+GGG 0.212139 5.3:+4228959 MS.gene072808:CDS
TGTAACAGAAGGGAAAATTT+TGG 0.225929 5.3:+4228816 MS.gene072808:CDS
GCTAATGAAACATTCCTTAT+AGG 0.235307 5.3:-4228847 None:intergenic
GTGTTTATTTCTAGGGATTT+CGG 0.268121 5.3:+4228335 MS.gene072808:intron
AAGCTGTTTGGGGTTTCAAT+TGG 0.272470 5.3:+4228970 MS.gene072808:CDS
CCTCATGTTCCTAAGCTGTT+TGG 0.282001 5.3:+4228958 MS.gene072808:CDS
ATGATGAGTAATTATGCTTC+TGG 0.326687 5.3:+4228739 MS.gene072808:CDS
ATGGAACAAGCTTCATAGTT+TGG 0.332696 5.3:+4228131 MS.gene072808:CDS
CCGTTGATGATTTGGTTTCA+TGG 0.341139 5.3:+4228101 MS.gene072808:CDS
ATTGAGTTGACGAACGAAAC+TGG 0.358502 5.3:-4228212 None:intergenic
ATGGTGTCTCCAGCAACTTC+TGG 0.358649 5.3:+4228529 MS.gene072808:CDS
TGATTCTTCCGTTGATGATT+TGG 0.364300 5.3:+4228093 MS.gene072808:CDS
GAAGTTGATGGCGACATTAC+CGG 0.375837 5.3:+4227985 None:intergenic
AGCTGTTTGGGGTTTCAATT+GGG 0.377393 5.3:+4228971 MS.gene072808:CDS
AGCAACTTCTGGTGATGAAC+AGG 0.397317 5.3:+4228540 MS.gene072808:CDS
TTATTGTGTTTGAAATACTT+TGG 0.401377 5.3:-4228184 None:intergenic
TGCTGCGACGGCACGGGGCG+TGG 0.405775 5.3:+4228792 MS.gene072808:CDS
CTTAATGTGGGTGGAGCTGC+TGG 0.418056 5.3:+4228886 MS.gene072808:CDS
TACGAGTGCTGCGACGGCAC+GGG 0.438079 5.3:+4228786 MS.gene072808:CDS
GACGGAGAATGAGGTTGAAC+CGG 0.444512 5.3:+4229008 MS.gene072808:CDS
ACATGAGGTGCCTCAACCTC+TGG 0.453623 5.3:-4228943 None:intergenic
CGGATGATCAAGAGCGTCGT+TGG 0.460641 5.3:+4229160 MS.gene072808:CDS
ATGTGAGACGACGAGTTTGA+TGG 0.466931 5.3:+4228918 MS.gene072808:CDS
GCCATAGCTGTTCCTGCTGC+CGG 0.467534 5.3:-4228451 None:intergenic
TTGGTTTCATGGAATGAAGA+TGG 0.472004 5.3:+4228112 MS.gene072808:CDS
TGTTCATCACCAGAAGTTGC+TGG 0.476217 5.3:-4228538 None:intergenic
GCACCATTGTTCACCGCAAT+CGG 0.477786 5.3:-4228583 None:intergenic
GATTCACCTGTTTGCTCCAC+CGG 0.494684 5.3:-4228004 None:intergenic
ACATTACCGGTGGAGCAAAC+AGG 0.498008 5.3:+4227998 MS.gene072808:CDS
TGAACCGGAGAGAGAGGAAC+GGG 0.498202 5.3:+4229023 MS.gene072808:CDS
GCTGGAGACACCATTCTAAC+CGG 0.500615 5.3:-4228520 None:intergenic
GTTGGAAATTGACGAAATAG+TGG 0.503816 5.3:+4229178 MS.gene072808:CDS
GTCGAATTCATCTCCGATTG+CGG 0.505685 5.3:+4228570 MS.gene072808:CDS
ATTGAAACCCCAAACAGCTT+AGG 0.506033 5.3:-4228967 None:intergenic
CAATTGGGCTTAAACGATGT+AGG 0.509913 5.3:+4228986 MS.gene072808:CDS
TTAGCAAATTCCCACCGGTC+AGG 0.510367 5.3:-4228367 None:intergenic
TCATTCTCTTCAACTAACTC+CGG 0.512986 5.3:-4228640 None:intergenic
TTCGGAAAGTTGTACCTGAC+CGG 0.514506 5.3:+4228353 MS.gene072808:CDS
CTACGAGTGCTGCGACGGCA+CGG 0.515613 5.3:+4228785 MS.gene072808:CDS
ATCTTCTACGAGTGCTGCGA+CGG 0.517274 5.3:+4228780 MS.gene072808:CDS
TGTTCCCGTTCCTCTCTCTC+CGG 0.518493 5.3:-4229027 None:intergenic
TATGGCGGCACCGGTTAGAA+TGG 0.532149 5.3:+4228510 MS.gene072808:CDS
TCATGTTCCTAAGCTGTTTG+GGG 0.536796 5.3:+4228960 MS.gene072808:CDS
AACAGTAGCTATGGCGGCAC+CGG 0.542259 5.3:+4228501 MS.gene072808:CDS
TGAGGTTGAACCGGAGAGAG+AGG 0.546548 5.3:+4229017 MS.gene072808:CDS
CATGAGTTGCACCACTGCGC+CGG 0.546633 5.3:+4228621 MS.gene072808:CDS
CGGTGGGAATTTGCTAATGA+CGG 0.547176 5.3:+4228373 MS.gene072808:CDS
GGTGGAGCAAACAGGTGAAT+CGG 0.547365 5.3:+4228006 MS.gene072808:CDS
GCCGGCAGCAGGAACAGCTA+TGG 0.547975 5.3:+4228450 MS.gene072808:CDS
TAATACTGTAACAGTAGCTA+TGG 0.548334 5.3:+4228492 MS.gene072808:CDS
ACGATGTAGGACGGAGAATG+AGG 0.556638 5.3:+4228999 MS.gene072808:CDS
ACGAGTGCTGCGACGGCACG+GGG 0.561173 5.3:+4228787 MS.gene072808:CDS
TTTGATGGAACCAGAGGTTG+AGG 0.561367 5.3:+4228933 MS.gene072808:CDS
AAATCTCTAGCAAATTCAGC+AGG 0.569125 5.3:-4228157 None:intergenic
TGGTGATGGTGATGGTGACT+CGG 0.574712 5.3:+4229140 MS.gene072808:CDS
TCTCCGATTGCGGTGAACAA+TGG 0.579507 5.3:+4228580 MS.gene072808:CDS
CGGGGCGTGGTTGTAACAGA+AGG 0.579523 5.3:+4228805 MS.gene072808:CDS
AACAGGTGAATCGGCGACGG+CGG 0.583900 5.3:+4228015 MS.gene072808:CDS
AATGAGCTAAGTCAATTGAA+AGG 0.584859 5.3:+4228694 MS.gene072808:CDS
AAGGAATGTTTCATTAGCTG+AGG 0.585509 5.3:+4228852 MS.gene072808:CDS
CCTCGTGATGGTGATGGTGA+TGG 0.588325 5.3:+4229132 MS.gene072808:CDS
ATCAAACTCGTCGTCTCACA+TGG 0.592517 5.3:-4228916 None:intergenic
GCAAACAGGTGAATCGGCGA+CGG 0.608757 5.3:+4228012 MS.gene072808:CDS
AACTTCTGGTGATGAACAGG+TGG 0.612326 5.3:+4228543 MS.gene072808:CDS
AGTTAGTTGAAGAGAATGAA+AGG 0.612844 5.3:+4228644 MS.gene072808:CDS
CCATGAAACCAAATCATCAA+CGG 0.613347 5.3:-4228101 None:intergenic
GGAAAGTTGTACCTGACCGG+TGG 0.614886 5.3:+4228356 MS.gene072808:CDS
TCTGAACCTCGTGATGGTGA+TGG 0.617342 5.3:+4229126 MS.gene072808:CDS
ATTTGCTTCGAGATATTCAG+CGG 0.621869 5.3:+4228416 MS.gene072808:CDS
TTCAACTAACTCCGGCGCAG+TGG 0.622278 5.3:-4228632 None:intergenic
GATGCAAACACAATCCTCGC+AGG 0.623759 5.3:+4229077 MS.gene072808:CDS
GCAGGAGACTGATCGAGGAT+CGG 0.626303 5.3:+4229095 MS.gene072808:CDS
TCCTCGCAGGAGACTGATCG+AGG 0.628520 5.3:+4229090 MS.gene072808:CDS
GAAAGTTGTACCTGACCGGT+GGG 0.631205 5.3:+4228357 MS.gene072808:CDS
TACTGTAACAGTAGCTATGG+CGG 0.634074 5.3:+4228495 MS.gene072808:CDS
AATGTGGGTGGAGCTGCTGG+CGG 0.634893 5.3:+4228889 MS.gene072808:CDS
GGAAAATGTAGTGCTTAATG+TGG 0.635559 5.3:+4228873 MS.gene072808:CDS
TTGAACCGGAGAGAGAGGAA+CGG 0.637385 5.3:+4229022 MS.gene072808:CDS
AATGTAGTGCTTAATGTGGG+TGG 0.639772 5.3:+4228877 MS.gene072808:CDS
GAAAATGTAGTGCTTAATGT+GGG 0.644997 5.3:+4228874 MS.gene072808:CDS
CGTCATTAGCAAATTCCCAC+CGG 0.646656 5.3:-4228372 None:intergenic
GACGAGTTTGATGGAACCAG+AGG 0.648812 5.3:+4228927 MS.gene072808:CDS
CCAAACAGCTTAGGAACATG+AGG 0.651312 5.3:-4228958 None:intergenic
TGGGCTTAAACGATGTAGGA+CGG 0.657843 5.3:+4228990 MS.gene072808:CDS
TCCTCGATCAGTCTCCTGCG+AGG 0.667916 5.3:-4229091 None:intergenic
AGGAACAGCTATGGCGACAG+CGG 0.668564 5.3:+4228459 MS.gene072808:CDS
GGGGCGTGGTTGTAACAGAA+GGG 0.668865 5.3:+4228806 MS.gene072808:CDS
GTTGATGGCGACATTACCGG+TGG 0.679162 5.3:+4227988 None:intergenic
GCTAATGACGGATTTCGACG+AGG 0.685443 5.3:+4228385 MS.gene072808:CDS
GTGAAATCTGAACCTCGTGA+TGG 0.700758 5.3:+4229120 MS.gene072808:CDS
CCATCACCATCACCATCACG+AGG 0.701913 5.3:-4229132 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAGAAAGTAAAACAAA+AGG - chr5.3:4228289-4228308 None:intergenic 15.0%
!!! AAATTTTGTGTTTATTTCTA+GGG + chr5.3:4228328-4228347 MS.gene072808:intron 15.0%
!!! TAAATTTTGTGTTTATTTCT+AGG + chr5.3:4228327-4228346 MS.gene072808:intron 15.0%
!!! TTTGTTTTACTTTCTTTTTT+AGG + chr5.3:4228289-4228308 MS.gene072808:intron 15.0%
!!! TTATTGTGTTTGAAATACTT+TGG - chr5.3:4228187-4228206 None:intergenic 20.0%
AATGAGCTAAGTCAATTGAA+AGG + chr5.3:4228694-4228713 MS.gene072808:CDS 30.0%
AGTTAGTTGAAGAGAATGAA+AGG + chr5.3:4228644-4228663 MS.gene072808:CDS 30.0%
ATGATGAGTAATTATGCTTC+TGG + chr5.3:4228739-4228758 MS.gene072808:CDS 30.0%
GAAAATGTAGTGCTTAATGT+GGG + chr5.3:4228874-4228893 MS.gene072808:CDS 30.0%
GCTAATGAAACATTCCTTAT+AGG - chr5.3:4228850-4228869 None:intergenic 30.0%
TAATACTGTAACAGTAGCTA+TGG + chr5.3:4228492-4228511 MS.gene072808:CDS 30.0%
! TGTAACAGAAGGGAAAATTT+TGG + chr5.3:4228816-4228835 MS.gene072808:CDS 30.0%
! TTTTGGATCTTTTGCCTATA+AGG + chr5.3:4228833-4228852 MS.gene072808:CDS 30.0%
!! GTGTTTATTTCTAGGGATTT+CGG + chr5.3:4228335-4228354 MS.gene072808:intron 30.0%
AAATCTCTAGCAAATTCAGC+AGG - chr5.3:4228160-4228179 None:intergenic 35.0%
AAGGAATGTTTCATTAGCTG+AGG + chr5.3:4228852-4228871 MS.gene072808:CDS 35.0%
ATGGAACAAGCTTCATAGTT+TGG + chr5.3:4228131-4228150 MS.gene072808:CDS 35.0%
ATTTGCTTCGAGATATTCAG+CGG + chr5.3:4228416-4228435 MS.gene072808:CDS 35.0%
CCATGAAACCAAATCATCAA+CGG - chr5.3:4228104-4228123 None:intergenic 35.0%
GGAAAATGTAGTGCTTAATG+TGG + chr5.3:4228873-4228892 MS.gene072808:CDS 35.0%
TCATTCTCTTCAACTAACTC+CGG - chr5.3:4228643-4228662 None:intergenic 35.0%
TTGGTTTCATGGAATGAAGA+TGG + chr5.3:4228112-4228131 MS.gene072808:CDS 35.0%
! TGATACGTTTTCGTCAGAAA+CGG - chr5.3:4228064-4228083 None:intergenic 35.0%
!! TGATTCTTCCGTTGATGATT+TGG + chr5.3:4228093-4228112 MS.gene072808:CDS 35.0%
AATGTAGTGCTTAATGTGGG+TGG + chr5.3:4228877-4228896 MS.gene072808:CDS 40.0%
ATTGAAACCCCAAACAGCTT+AGG - chr5.3:4228970-4228989 None:intergenic 40.0%
CAATTGGGCTTAAACGATGT+AGG + chr5.3:4228986-4229005 MS.gene072808:CDS 40.0%
CTCATGTTCCTAAGCTGTTT+GGG + chr5.3:4228959-4228978 MS.gene072808:CDS 40.0%
TACTGTAACAGTAGCTATGG+CGG + chr5.3:4228495-4228514 MS.gene072808:CDS 40.0%
TCAGCGGAGAAAAATATTGC+CGG + chr5.3:4228432-4228451 MS.gene072808:CDS 40.0%
TCATGTTCCTAAGCTGTTTG+GGG + chr5.3:4228960-4228979 MS.gene072808:CDS 40.0%
! AAGCTGTTTGGGGTTTCAAT+TGG + chr5.3:4228970-4228989 MS.gene072808:CDS 40.0%
! AGCTGTTTGGGGTTTCAATT+GGG + chr5.3:4228971-4228990 MS.gene072808:CDS 40.0%
!! ATTGAGTTGACGAACGAAAC+TGG - chr5.3:4228215-4228234 None:intergenic 40.0%
!! CCGTTGATGATTTGGTTTCA+TGG + chr5.3:4228101-4228120 MS.gene072808:CDS 40.0%
AGAAAAATATTGCCGGCAGC+AGG + chr5.3:4228439-4228458 MS.gene072808:CDS 45.0%
ATCAAACTCGTCGTCTCACA+TGG - chr5.3:4228919-4228938 None:intergenic 45.0%
ATGTGAGACGACGAGTTTGA+TGG + chr5.3:4228918-4228937 MS.gene072808:CDS 45.0%
CCAAACAGCTTAGGAACATG+AGG - chr5.3:4228961-4228980 None:intergenic 45.0%
CCTCATGTTCCTAAGCTGTT+TGG + chr5.3:4228958-4228977 MS.gene072808:CDS 45.0%
CGTCATTAGCAAATTCCCAC+CGG - chr5.3:4228375-4228394 None:intergenic 45.0%
GTCGAATTCATCTCCGATTG+CGG + chr5.3:4228570-4228589 MS.gene072808:CDS 45.0%
GTGAAATCTGAACCTCGTGA+TGG + chr5.3:4229120-4229139 MS.gene072808:CDS 45.0%
TGGGCTTAAACGATGTAGGA+CGG + chr5.3:4228990-4229009 MS.gene072808:CDS 45.0%
TGTTCATCACCAGAAGTTGC+TGG - chr5.3:4228541-4228560 None:intergenic 45.0%
TTCGGAAAGTTGTACCTGAC+CGG + chr5.3:4228353-4228372 MS.gene072808:CDS 45.0%
TTTGATGGAACCAGAGGTTG+AGG + chr5.3:4228933-4228952 MS.gene072808:CDS 45.0%
! AACTTCTGGTGATGAACAGG+TGG + chr5.3:4228543-4228562 MS.gene072808:CDS 45.0%
! AGCAACTTCTGGTGATGAAC+AGG + chr5.3:4228540-4228559 MS.gene072808:CDS 45.0%
!! CGGTGGGAATTTGCTAATGA+CGG + chr5.3:4228373-4228392 MS.gene072808:CDS 45.0%
ACATTACCGGTGGAGCAAAC+AGG + chr5.3:4227998-4228017 MS.gene072808:CDS 50.0%
ACGATGTAGGACGGAGAATG+AGG + chr5.3:4228999-4229018 MS.gene072808:CDS 50.0%
ATGGTGTCTCCAGCAACTTC+TGG + chr5.3:4228529-4228548 MS.gene072808:CDS 50.0%
GAAAGTTGTACCTGACCGGT+GGG + chr5.3:4228357-4228376 MS.gene072808:CDS 50.0%
GACGAGTTTGATGGAACCAG+AGG + chr5.3:4228927-4228946 MS.gene072808:CDS 50.0%
GACGGAGAATGAGGTTGAAC+CGG + chr5.3:4229008-4229027 MS.gene072808:CDS 50.0%
GATGCAAACACAATCCTCGC+AGG + chr5.3:4229077-4229096 MS.gene072808:CDS 50.0%
GATTCACCTGTTTGCTCCAC+CGG - chr5.3:4228007-4228026 None:intergenic 50.0%
GCACCATTGTTCACCGCAAT+CGG - chr5.3:4228586-4228605 None:intergenic 50.0%
GCTGGAGACACCATTCTAAC+CGG - chr5.3:4228523-4228542 None:intergenic 50.0%
GGTGGAGCAAACAGGTGAAT+CGG + chr5.3:4228006-4228025 MS.gene072808:CDS 50.0%
TCTCCGATTGCGGTGAACAA+TGG + chr5.3:4228580-4228599 MS.gene072808:CDS 50.0%
TTAGCAAATTCCCACCGGTC+AGG - chr5.3:4228370-4228389 None:intergenic 50.0%
TTGAACCGGAGAGAGAGGAA+CGG + chr5.3:4229022-4229041 MS.gene072808:CDS 50.0%
! ATCTTCTACGAGTGCTGCGA+CGG + chr5.3:4228780-4228799 MS.gene072808:CDS 50.0%
! GTTTTCGTCAGAAACGGCGT+CGG - chr5.3:4228058-4228077 None:intergenic 50.0%
! TCTGAACCTCGTGATGGTGA+TGG + chr5.3:4229126-4229145 MS.gene072808:CDS 50.0%
!! GCTAATGACGGATTTCGACG+AGG + chr5.3:4228385-4228404 MS.gene072808:CDS 50.0%
!! TGGTGATGGTGATGGTGACT+CGG + chr5.3:4229140-4229159 MS.gene072808:CDS 50.0%
AACAGTAGCTATGGCGGCAC+CGG + chr5.3:4228501-4228520 MS.gene072808:CDS 55.0%
AGGAACAGCTATGGCGACAG+CGG + chr5.3:4228459-4228478 MS.gene072808:CDS 55.0%
CCATCACCATCACCATCACG+AGG - chr5.3:4229135-4229154 None:intergenic 55.0%
CTTAATGTGGGTGGAGCTGC+TGG + chr5.3:4228886-4228905 MS.gene072808:CDS 55.0%
GCAAACAGGTGAATCGGCGA+CGG + chr5.3:4228012-4228031 MS.gene072808:CDS 55.0%
GCAGGAGACTGATCGAGGAT+CGG + chr5.3:4229095-4229114 MS.gene072808:CDS 55.0%
GGAAAGTTGTACCTGACCGG+TGG + chr5.3:4228356-4228375 MS.gene072808:CDS 55.0%
GGGGCGTGGTTGTAACAGAA+GGG + chr5.3:4228806-4228825 MS.gene072808:CDS 55.0%
TGAACCGGAGAGAGAGGAAC+GGG + chr5.3:4229023-4229042 MS.gene072808:CDS 55.0%
TGAGGTTGAACCGGAGAGAG+AGG + chr5.3:4229017-4229036 MS.gene072808:CDS 55.0%
TGTTCCCGTTCCTCTCTCTC+CGG - chr5.3:4229030-4229049 None:intergenic 55.0%
TTCAACTAACTCCGGCGCAG+TGG - chr5.3:4228635-4228654 None:intergenic 55.0%
! ACATGAGGTGCCTCAACCTC+TGG - chr5.3:4228946-4228965 None:intergenic 55.0%
!! CCTCGTGATGGTGATGGTGA+TGG + chr5.3:4229132-4229151 MS.gene072808:CDS 55.0%
!! CGGATGATCAAGAGCGTCGT+TGG + chr5.3:4229160-4229179 MS.gene072808:CDS 55.0%
!! TATGGCGGCACCGGTTAGAA+TGG + chr5.3:4228510-4228529 MS.gene072808:CDS 55.0%
AACAGGTGAATCGGCGACGG+CGG + chr5.3:4228015-4228034 MS.gene072808:CDS 60.0%
AATGTGGGTGGAGCTGCTGG+CGG + chr5.3:4228889-4228908 MS.gene072808:CDS 60.0%
CATGAGTTGCACCACTGCGC+CGG + chr5.3:4228621-4228640 MS.gene072808:CDS 60.0%
CGGGGCGTGGTTGTAACAGA+AGG + chr5.3:4228805-4228824 MS.gene072808:CDS 60.0%
GCCATAGCTGTTCCTGCTGC+CGG - chr5.3:4228454-4228473 None:intergenic 60.0%
TCCTCGATCAGTCTCCTGCG+AGG - chr5.3:4229094-4229113 None:intergenic 60.0%
TCCTCGCAGGAGACTGATCG+AGG + chr5.3:4229090-4229109 MS.gene072808:CDS 60.0%
GCCGGCAGCAGGAACAGCTA+TGG + chr5.3:4228450-4228469 MS.gene072808:CDS 65.0%
! CTACGAGTGCTGCGACGGCA+CGG + chr5.3:4228785-4228804 MS.gene072808:CDS 65.0%
! TACGAGTGCTGCGACGGCAC+GGG + chr5.3:4228786-4228805 MS.gene072808:CDS 65.0%
! ACGAGTGCTGCGACGGCACG+GGG + chr5.3:4228787-4228806 MS.gene072808:CDS 70.0%
! TGCTGCGACGGCACGGGGCG+TGG + chr5.3:4228792-4228811 MS.gene072808:CDS 80.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 4227992 4229197 4227992 ID=MS.gene072808
chr5.3 mRNA 4227992 4229197 4227992 ID=MS.gene072808.t1;Parent=MS.gene072808
chr5.3 exon 4227992 4228240 4227992 ID=MS.gene072808.t1.exon1;Parent=MS.gene072808.t1
chr5.3 CDS 4227992 4228240 4227992 ID=cds.MS.gene072808.t1;Parent=MS.gene072808.t1
chr5.3 exon 4228349 4229197 4228349 ID=MS.gene072808.t1.exon2;Parent=MS.gene072808.t1
chr5.3 CDS 4228349 4229197 4228349 ID=cds.MS.gene072808.t1;Parent=MS.gene072808.t1
Gene Sequence

>MS.gene072808

ATGGCGACATTACCGGTGGAGCAAACAGGTGAATCGGCGACGGCGGAGTCGCAGAGATCGATTCCGACGCCGTTTCTGACGAAAACGTATCAACTTGTTGATGATTCTTCCGTTGATGATTTGGTTTCATGGAATGAAGATGGAACAAGCTTCATAGTTTGGAAGCCTGCTGAATTTGCTAGAGATTTGCTTCCAAAGTATTTCAAACACAATAATTTCTCCAGTTTCGTTCGTCAACTCAATACTTATGTAACTAACTAAATCTCTTTAATCTTAATCTTATCGTATTCTATTCCTTTTGTTTTACTTTCTTTTTTAGGATTTTTTTTTCTCTCTAAATTTTGTGTTTATTTCTAGGGATTTCGGAAAGTTGTACCTGACCGGTGGGAATTTGCTAATGACGGATTTCGACGAGGCGAGAAAAATTTGCTTCGAGATATTCAGCGGAGAAAAATATTGCCGGCAGCAGGAACAGCTATGGCGACAGCGGTAGCTGCAGCTAATACTGTAACAGTAGCTATGGCGGCACCGGTTAGAATGGTGTCTCCAGCAACTTCTGGTGATGAACAGGTGGTTTCGTCGAATTCATCTCCGATTGCGGTGAACAATGGTGCTACAGTGCAGCGTAGCATGAGTTGCACCACTGCGCCGGAGTTAGTTGAAGAGAATGAAAGGCTTAAGAAAGAGAACATGCAATTGAGTAATGAGCTAAGTCAATTGAAAGGTCTTTGTAATAATATACTTGCTATGATGAGTAATTATGCTTCTGGTTTTAGTAGACAGTTAGAATCTTCTACGAGTGCTGCGACGGCACGGGGCGTGGTTGTAACAGAAGGGAAAATTTTGGATCTTTTGCCTATAAGGAATGTTTCATTAGCTGAGGAAAATGTAGTGCTTAATGTGGGTGGAGCTGCTGGCGGTGTGCCATGTGAGACGACGAGTTTGATGGAACCAGAGGTTGAGGCACCTCATGTTCCTAAGCTGTTTGGGGTTTCAATTGGGCTTAAACGATGTAGGACGGAGAATGAGGTTGAACCGGAGAGAGAGGAACGGGAACAGCATCAACAAATTCGAATGCAAGTGCAGATGCAAACACAATCCTCGCAGGAGACTGATCGAGGATCGGATGTGAAATCTGAACCTCGTGATGGTGATGGTGATGGTGACTCGGATGATCAAGAGCGTCGTTGGAAATTGACGAAATAG
Protein sequence

>MS.gene072808.t1

MATLPVEQTGESATAESQRSIPTPFLTKTYQLVDDSSVDDLVSWNEDGTSFIVWKPAEFARDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDGFRRGEKNLLRDIQRRKILPAAGTAMATAVAAANTVTVAMAAPVRMVSPATSGDEQVVSSNSSPIAVNNGATVQRSMSCTTAPELVEENERLKKENMQLSNELSQLKGLCNNILAMMSNYASGFSRQLESSTSAATARGVVVTEGKILDLLPIRNVSLAEENVVLNVGGAAGGVPCETTSLMEPEVEAPHVPKLFGVSIGLKRCRTENEVEPEREEREQHQQIRMQVQMQTQSSQETDRGSDVKSEPRDGDGDGDSDDQERRWKLTK