Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053590.t1 | XP_003608377.2 | 97.7 | 264 | 6 | 0 | 1 | 264 | 1 | 264 | 1.00E-149 | 539.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053590.t1 | O82225 | 51.0 | 247 | 119 | 1 | 4 | 248 | 1 | 247 | 4.7e-71 | 269.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053590.t1 | G7JV26 | 97.7 | 264 | 6 | 0 | 1 | 264 | 1 | 264 | 7.4e-150 | 539.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049653 | MS.gene053590 | 0.814068 | 1.86E-51 | -1.69E-46 |
| MS.gene050384 | MS.gene053590 | 0.818338 | 2.07E-52 | -1.69E-46 |
| MS.gene050386 | MS.gene053590 | 0.826443 | 2.70E-54 | -1.69E-46 |
| MS.gene050437 | MS.gene053590 | 0.802559 | 5.30E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene053590 | 0.825491 | 4.55E-54 | -1.69E-46 |
| MS.gene050752 | MS.gene053590 | 0.822945 | 1.80E-53 | -1.69E-46 |
| MS.gene051003 | MS.gene053590 | 0.80584 | 1.10E-49 | -1.69E-46 |
| MS.gene051328 | MS.gene053590 | 0.825813 | 3.82E-54 | -1.69E-46 |
| MS.gene051343 | MS.gene053590 | 0.806158 | 9.42E-50 | -1.69E-46 |
| MS.gene052947 | MS.gene053590 | 0.808766 | 2.64E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene054403 | 0.81877 | 1.65E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene057234 | 0.81799 | 2.48E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene057392 | 0.811716 | 6.09E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene057694 | 0.805096 | 1.57E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene058054 | 0.81594 | 7.14E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene058055 | 0.840873 | 6.69E-58 | -1.69E-46 |
| MS.gene053590 | MS.gene061878 | 0.810659 | 1.03E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene063026 | 0.824736 | 6.87E-54 | -1.69E-46 |
| MS.gene053590 | MS.gene064071 | 0.813165 | 2.94E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene064072 | 0.818097 | 2.34E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene06620 | 0.800857 | 1.18E-48 | -1.69E-46 |
| MS.gene053590 | MS.gene066718 | 0.807527 | 4.84E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene068013 | 0.838559 | 2.68E-57 | -1.69E-46 |
| MS.gene053590 | MS.gene068166 | 0.821326 | 4.29E-53 | -1.69E-46 |
| MS.gene053590 | MS.gene06836 | 0.846329 | 2.33E-59 | -1.69E-46 |
| MS.gene053590 | MS.gene06837 | 0.817404 | 3.36E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene069828 | 0.811778 | 5.91E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene06984 | 0.80553 | 1.28E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene06989 | 0.835906 | 1.28E-56 | -1.69E-46 |
| MS.gene053590 | MS.gene070244 | 0.809153 | 2.18E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene07090 | 0.830031 | 3.69E-55 | -1.69E-46 |
| MS.gene053590 | MS.gene07100 | 0.802396 | 5.72E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene07217 | 0.813646 | 2.30E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene07222 | 0.814909 | 1.21E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene072386 | 0.819056 | 1.42E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene072555 | 0.8009 | 1.16E-48 | -1.69E-46 |
| MS.gene053590 | MS.gene072808 | 0.810158 | 1.32E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene073674 | 0.824783 | 6.69E-54 | -1.69E-46 |
| MS.gene053590 | MS.gene073954 | 0.804171 | 2.46E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene08428 | 0.816916 | 4.32E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene08793 | 0.802592 | 5.21E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene08889 | 0.803563 | 3.28E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene08948 | 0.807818 | 4.20E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene09032 | 0.816987 | 4.16E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene20302 | 0.804309 | 2.30E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene20740 | 0.814785 | 1.29E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene20886 | 0.80447 | 2.13E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene21426 | 0.833262 | 5.90E-56 | -1.69E-46 |
| MS.gene053590 | MS.gene22499 | 0.825315 | 5.01E-54 | -1.69E-46 |
| MS.gene053590 | MS.gene22740 | 0.81183 | 5.75E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene22968 | 0.837997 | 3.74E-57 | -1.69E-46 |
| MS.gene053590 | MS.gene24610 | 0.82343 | 1.39E-53 | -1.69E-46 |
| MS.gene053590 | MS.gene24930 | 0.817039 | 4.05E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene29891 | 0.817294 | 3.55E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene35034 | 0.815036 | 1.14E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene35048 | 0.82994 | 3.89E-55 | -1.69E-46 |
| MS.gene053590 | MS.gene35214 | 0.804914 | 1.72E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene39586 | 0.804285 | 2.32E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene40591 | 0.812863 | 3.42E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene41775 | 0.814206 | 1.73E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene41867 | 0.834755 | 2.49E-56 | -1.69E-46 |
| MS.gene053590 | MS.gene42302 | 0.836725 | 7.91E-57 | -1.69E-46 |
| MS.gene053590 | MS.gene43537 | 0.804802 | 1.81E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene43602 | 0.806046 | 9.95E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene47311 | 0.82146 | 3.99E-53 | -1.69E-46 |
| MS.gene053590 | MS.gene49816 | 0.820827 | 5.58E-53 | -1.69E-46 |
| MS.gene053590 | MS.gene50775 | 0.827748 | 1.32E-54 | -1.69E-46 |
| MS.gene053590 | MS.gene52837 | 0.825889 | 3.66E-54 | -1.69E-46 |
| MS.gene053590 | MS.gene54919 | 0.804457 | 2.14E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene55094 | 0.829112 | 6.18E-55 | -1.69E-46 |
| MS.gene053590 | MS.gene55887 | 0.811173 | 7.99E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene56451 | 0.800772 | 1.23E-48 | -1.69E-46 |
| MS.gene053590 | MS.gene56656 | 0.805632 | 1.22E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene58098 | 0.810267 | 1.25E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene58438 | 0.801727 | 7.85E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene61001 | 0.801039 | 1.09E-48 | -1.69E-46 |
| MS.gene053590 | MS.gene65518 | 0.828859 | 7.11E-55 | -1.69E-46 |
| MS.gene053590 | MS.gene68136 | 0.828532 | 8.53E-55 | -1.69E-46 |
| MS.gene053590 | MS.gene68735 | 0.824702 | 7.00E-54 | -1.69E-46 |
| MS.gene053590 | MS.gene68810 | 0.80392 | 2.77E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene70398 | 0.829233 | 5.77E-55 | -1.69E-46 |
| MS.gene053590 | MS.gene71215 | 0.8074 | 5.15E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene71616 | 0.830078 | 3.59E-55 | -1.69E-46 |
| MS.gene053590 | MS.gene71994 | 0.812715 | 3.69E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene74458 | 0.838814 | 2.30E-57 | -1.69E-46 |
| MS.gene053590 | MS.gene75148 | 0.827289 | 1.70E-54 | -1.69E-46 |
| MS.gene053590 | MS.gene82841 | 0.807675 | 4.50E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene82938 | 0.800433 | 1.44E-48 | -1.69E-46 |
| MS.gene053590 | MS.gene87972 | 0.802975 | 4.35E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene88976 | 0.815493 | 8.99E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene89648 | 0.801812 | 7.55E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene89677 | 0.846238 | 2.46E-59 | -1.69E-46 |
| MS.gene053590 | MS.gene90232 | 0.873727 | 1.17E-67 | -1.69E-46 |
| MS.gene053590 | MS.gene90861 | 0.823312 | 1.48E-53 | -1.69E-46 |
| MS.gene053590 | MS.gene90981 | 0.826819 | 2.20E-54 | -1.69E-46 |
| MS.gene053590 | MS.gene91203 | 0.807179 | 5.73E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene91307 | 0.804386 | 2.22E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene92572 | 0.802009 | 6.88E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene94952 | 0.818825 | 1.60E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene99313 | 0.805429 | 1.34E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene053590 | MS.gene028495 | PPI |
| MS.gene002251 | MS.gene053590 | PPI |
| MS.gene053590 | MS.gene01224 | PPI |
| MS.gene071221 | MS.gene053590 | PPI |
| MS.gene053590 | MS.gene002251 | PPI |
| MS.gene90232 | MS.gene053590 | PPI |
| MS.gene94216 | MS.gene053590 | PPI |
| MS.gene76089 | MS.gene053590 | PPI |
| MS.gene068013 | MS.gene053590 | PPI |
| MS.gene053590 | MS.gene02208 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053590.t1 | MTR_4g093250 | 97.727 | 264 | 6 | 0 | 1 | 264 | 1 | 264 | 0.0 | 533 |
| MS.gene053590.t1 | MTR_5g083210 | 72.289 | 249 | 65 | 2 | 1 | 249 | 1 | 245 | 1.36e-133 | 377 |
| MS.gene053590.t1 | MTR_2g009720 | 60.000 | 240 | 93 | 1 | 9 | 248 | 8 | 244 | 2.55e-102 | 298 |
| MS.gene053590.t1 | MTR_2g009720 | 59.903 | 207 | 80 | 1 | 9 | 215 | 8 | 211 | 3.85e-88 | 262 |
| MS.gene053590.t1 | MTR_5g078650 | 58.000 | 50 | 18 | 1 | 42 | 91 | 54 | 100 | 7.66e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053590.t1 | AT2G23970 | 51.012 | 247 | 119 | 1 | 4 | 248 | 1 | 247 | 5.65e-91 | 270 |
| MS.gene053590.t1 | AT4G30550 | 46.559 | 247 | 129 | 1 | 4 | 247 | 3 | 249 | 1.03e-82 | 248 |
| MS.gene053590.t1 | AT4G30540 | 48.980 | 245 | 125 | 0 | 4 | 248 | 1 | 245 | 6.02e-81 | 244 |
| MS.gene053590.t1 | AT2G23960 | 44.672 | 244 | 133 | 1 | 5 | 246 | 1 | 244 | 1.86e-75 | 230 |
| MS.gene053590.t1 | AT4G30530 | 45.041 | 242 | 131 | 1 | 9 | 248 | 5 | 246 | 1.10e-74 | 228 |
| MS.gene053590.t1 | AT2G23960 | 46.635 | 208 | 109 | 1 | 5 | 210 | 1 | 208 | 2.46e-67 | 208 |
Find 77 sgRNAs with CRISPR-Local
Find 143 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGTACGGAGGGTATTT+TGG | 0.159436 | 4.4:-21484528 | MS.gene053590:CDS |
| TAGGGAGTGGGAAGAGATTT+GGG | 0.235069 | 4.4:-21484587 | MS.gene053590:CDS |
| CTCTTCCATGCCTCCTTATC+TGG | 0.241065 | 4.4:+21482681 | None:intergenic |
| TAGATATGGAGATCACATTA+TGG | 0.306527 | 4.4:-21482944 | MS.gene053590:CDS |
| GTAGGGAGTGGGAAGAGATT+TGG | 0.311768 | 4.4:-21484588 | MS.gene053590:CDS |
| TGGCATTTGCTTTGGTCATC+AGG | 0.316497 | 4.4:-21484298 | MS.gene053590:intron |
| GGAATAGAAAATATAAATAT+TGG | 0.322855 | 4.4:-21482615 | MS.gene053590:CDS |
| TGTTAATTTGATTAGATACT+TGG | 0.341295 | 4.4:-21483319 | MS.gene053590:intron |
| ATCCCTGTGGCATTGAATAA+TGG | 0.342555 | 4.4:+21483174 | None:intergenic |
| GGCTGAAGTGATTGCTAAAT+CGG | 0.357904 | 4.4:-21482989 | MS.gene053590:CDS |
| TAGATACTTGGCCGTGCAAT+TGG | 0.364461 | 4.4:-21483307 | MS.gene053590:CDS |
| GGAATTGAGATGTTTAGATA+TGG | 0.367188 | 4.4:-21482958 | MS.gene053590:CDS |
| AAAATCCTTGGCATTTGCTT+TGG | 0.382494 | 4.4:-21484306 | MS.gene053590:CDS |
| AGATTCAATTCATCATCCTT+TGG | 0.389173 | 4.4:+21484435 | None:intergenic |
| TGAAAGCTGGGATGTGTATA+AGG | 0.394224 | 4.4:-21484475 | MS.gene053590:CDS |
| AGATATGGAGATCACATTAT+GGG | 0.414653 | 4.4:-21482943 | MS.gene053590:CDS |
| GGTCGTTATTGTGAAAGAAA+TGG | 0.428460 | 4.4:-21482636 | MS.gene053590:CDS |
| TGGAGGAAGAAGGTGAAAGC+TGG | 0.433224 | 4.4:-21484488 | MS.gene053590:CDS |
| TTTCCATTATTCAATGCCAC+AGG | 0.461141 | 4.4:-21483177 | MS.gene053590:CDS |
| TGGAGAAAATGGTAGGGAGT+GGG | 0.479980 | 4.4:-21484599 | MS.gene053590:CDS |
| GACATGAACAAGAAAATCCT+TGG | 0.480295 | 4.4:-21484318 | MS.gene053590:CDS |
| AAGGTGACTCGATCTCCAAC+TGG | 0.484187 | 4.4:-21483280 | MS.gene053590:CDS |
| GATCACATTATGGGAATTCA+AGG | 0.489176 | 4.4:-21482934 | MS.gene053590:CDS |
| TGAAGGAGAAGGCTGGTATG+TGG | 0.491137 | 4.4:-21482707 | MS.gene053590:CDS |
| TGGGGTATTTGTGAGAATGT+TGG | 0.492454 | 4.4:-21484508 | MS.gene053590:CDS |
| GTGAGAATGTTGGAGGAAGA+AGG | 0.500993 | 4.4:-21484498 | MS.gene053590:CDS |
| TGAATAATGGAAAGACTCGT+TGG | 0.502242 | 4.4:+21483187 | None:intergenic |
| GCAATCACTTCAGCCTTGGC+AGG | 0.507796 | 4.4:+21482997 | None:intergenic |
| TGCTATGAGATTGAAGGAGA+AGG | 0.513622 | 4.4:-21482718 | MS.gene053590:CDS |
| GACAAAGGAGATGGCAAAGA+GGG | 0.513633 | 4.4:+21483223 | None:intergenic |
| TTAAGGATGGAGAAAATGGT+AGG | 0.514388 | 4.4:-21484606 | None:intergenic |
| TTTAGCAATCACTTCAGCCT+TGG | 0.516942 | 4.4:+21482993 | None:intergenic |
| AGGGTGATAATGTTATGTTT+CGG | 0.519404 | 4.4:+21483242 | None:intergenic |
| ATACTTGGCCGTGCAATTGG+AGG | 0.519944 | 4.4:-21483304 | MS.gene053590:CDS |
| GACTCGATCTCCAACTGGAT+GGG | 0.522832 | 4.4:-21483275 | MS.gene053590:CDS |
| TGGTATGTGGGAGCCAGATA+AGG | 0.526816 | 4.4:-21482694 | MS.gene053590:CDS |
| ATTGCTAAATCGGATAAGAC+TGG | 0.535654 | 4.4:-21482979 | MS.gene053590:CDS |
| ATGGAGAAAATGGTAGGGAG+TGG | 0.538240 | 4.4:-21484600 | MS.gene053590:CDS |
| TATTCAATGCCACAGGGATG+AGG | 0.540762 | 4.4:-21483170 | MS.gene053590:intron |
| TCTCCAACTGGATGGGACAT+TGG | 0.548672 | 4.4:-21483268 | MS.gene053590:CDS |
| GATGAATTGAATCTGTATGA+TGG | 0.549776 | 4.4:-21484426 | MS.gene053590:CDS |
| TGAAATTTCGCTGAATAAGA+CGG | 0.555005 | 4.4:+21482869 | None:intergenic |
| ATAGAAAATATAAATATTGG+TGG | 0.558147 | 4.4:-21482612 | MS.gene053590:CDS |
| TGACTCGATCTCCAACTGGA+TGG | 0.559221 | 4.4:-21483276 | MS.gene053590:CDS |
| GCTAAGAGAGCTACCTGCCA+AGG | 0.562053 | 4.4:-21483010 | MS.gene053590:CDS |
| TCAAGAGAAGACAAAGGAGA+TGG | 0.563452 | 4.4:+21483214 | None:intergenic |
| GGAGGAAGAAGGTGAAAGCT+GGG | 0.565836 | 4.4:-21484487 | MS.gene053590:CDS |
| ACTATGCATCAGCTTCCTCA+AGG | 0.566000 | 4.4:-21482658 | MS.gene053590:CDS |
| GAAGGAGAAGGCTGGTATGT+GGG | 0.577034 | 4.4:-21482706 | MS.gene053590:CDS |
| TAAGGATGGAGAAAATGGTA+GGG | 0.582135 | 4.4:-21484605 | None:intergenic |
| GAATATGTGAAGAAAATGTA+CGG | 0.585607 | 4.4:-21484540 | MS.gene053590:CDS |
| ACACCAATGTCCCATCCAGT+TGG | 0.587627 | 4.4:+21483265 | None:intergenic |
| TGGCCGTGCAATTGGAGGGA+AGG | 0.591128 | 4.4:-21483299 | MS.gene053590:CDS |
| TACTTGGCCGTGCAATTGGA+GGG | 0.593875 | 4.4:-21483303 | MS.gene053590:CDS |
| ATGAGATTGAAGGAGAAGGC+TGG | 0.598435 | 4.4:-21482714 | MS.gene053590:CDS |
| ATGTGAAGAAAATGTACGGA+GGG | 0.598596 | 4.4:-21484536 | MS.gene053590:CDS |
| AGACAAAGGAGATGGCAAAG+AGG | 0.600946 | 4.4:+21483222 | None:intergenic |
| ATGCACATGGAAATGATACA+TGG | 0.606888 | 4.4:-21484377 | MS.gene053590:CDS |
| TTCCATTATTCAATGCCACA+GGG | 0.608968 | 4.4:-21483176 | MS.gene053590:CDS |
| TCACCTTCCCTCCAATTGCA+CGG | 0.609277 | 4.4:+21483296 | None:intergenic |
| GGGAGCCAGATAAGGAGGCA+TGG | 0.620410 | 4.4:-21482686 | MS.gene053590:CDS |
| GGTATTTGTGAGAATGTTGG+AGG | 0.624181 | 4.4:-21484505 | MS.gene053590:CDS |
| GGAAGTTCAAGAGAAGACAA+AGG | 0.628531 | 4.4:+21483208 | None:intergenic |
| AGGGAGTGGGAAGAGATTTG+GGG | 0.635409 | 4.4:-21484586 | MS.gene053590:CDS |
| CTATGCATCAGCTTCCTCAA+GGG | 0.636887 | 4.4:-21482657 | MS.gene053590:CDS |
| AGAATGTCTTTAGAGTACTC+AGG | 0.643445 | 4.4:+21482907 | None:intergenic |
| TATGTGGGAGCCAGATAAGG+AGG | 0.645828 | 4.4:-21482691 | MS.gene053590:CDS |
| GATGACCAAAGCAAATGCCA+AGG | 0.653162 | 4.4:+21484301 | None:intergenic |
| GGCGCGTGGAGAGTTTCCAA+AGG | 0.654333 | 4.4:-21484451 | MS.gene053590:CDS |
| TATTCAGCGAAATTTCATCA+CGG | 0.662548 | 4.4:-21482863 | MS.gene053590:intron |
| TATGTGAAGAAAATGTACGG+AGG | 0.666492 | 4.4:-21484537 | MS.gene053590:CDS |
| TTTCACAATAACGACCCTTG+AGG | 0.678121 | 4.4:+21482643 | None:intergenic |
| TGCACATGGAAATGATACAT+GGG | 0.699531 | 4.4:-21484376 | MS.gene053590:CDS |
| GATGTGTATAAGGTGGCGCG+TGG | 0.734044 | 4.4:-21484465 | MS.gene053590:CDS |
| GGTAGTTGTAGTGATGCACA+TGG | 0.738222 | 4.4:-21484390 | MS.gene053590:CDS |
| ACACACACACCTCATCCCTG+TGG | 0.758837 | 4.4:+21483161 | None:intergenic |
| AAGCTGGGATGTGTATAAGG+TGG | 0.761338 | 4.4:-21484472 | MS.gene053590:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATAAATGAAGTAATAA+AGG | + | chr4.4:21484380-21484399 | None:intergenic | 10.0% |
| !!! | ATGATAATGTTAATTTAATA+AGG | + | chr4.4:21484081-21484100 | None:intergenic | 10.0% |
| !!! | ATGTTAATTATTATGATTTT+TGG | + | chr4.4:21483555-21483574 | None:intergenic | 10.0% |
| !!! | TTAAAATTTTGATATATTGA+AGG | - | chr4.4:21483300-21483319 | MS.gene053590:CDS | 10.0% |
| !! | ACCATTAATTAACTTTATAA+AGG | - | chr4.4:21483832-21483851 | MS.gene053590:intron | 15.0% |
| !! | AGTGATTTAATTACTAAATA+TGG | + | chr4.4:21482952-21482971 | None:intergenic | 15.0% |
| !! | ATAGAAAATATAAATATTGG+TGG | - | chr4.4:21484590-21484609 | MS.gene053590:CDS | 15.0% |
| !! | ATATTTAGTAATTAAATCAC+TGG | - | chr4.4:21482951-21482970 | MS.gene053590:CDS | 15.0% |
| !! | GGAATAGAAAATATAAATAT+TGG | - | chr4.4:21484587-21484606 | MS.gene053590:CDS | 15.0% |
| !! | GTTAAATATTAGTAATAGAT+AGG | + | chr4.4:21483523-21483542 | None:intergenic | 15.0% |
| !! | TCAAAAAAAAAAAAGGAATA+GGG | - | chr4.4:21483129-21483148 | MS.gene053590:intron | 15.0% |
| !! | TCCTTTATAAAGTTAATTAA+TGG | + | chr4.4:21483836-21483855 | None:intergenic | 15.0% |
| !! | TTCAAAAAAAAAAAAGGAAT+AGG | - | chr4.4:21483128-21483147 | MS.gene053590:intron | 15.0% |
| !!! | AATTTCTAAGGTATTTTTTT+GGG | + | chr4.4:21483355-21483374 | None:intergenic | 15.0% |
| !!! | TTTTTTTTAGAGGAAAAATA+GGG | - | chr4.4:21483198-21483217 | MS.gene053590:CDS | 15.0% |
| !!! | TTTTTTTTTAGAGGAAAAAT+AGG | - | chr4.4:21483197-21483216 | MS.gene053590:CDS | 15.0% |
| !! | AAAAAATGAAAGGTTTGAAA+TGG | + | chr4.4:21483007-21483026 | None:intergenic | 20.0% |
| !! | AAAATACCTTAGAAATTCTT+AGG | - | chr4.4:21483358-21483377 | MS.gene053590:intron | 20.0% |
| !! | AACAAAAATTACTAGTAGAA+AGG | + | chr4.4:21483031-21483050 | None:intergenic | 20.0% |
| !! | GCTAATTAATTAAATCCAAA+AGG | + | chr4.4:21484174-21484193 | None:intergenic | 20.0% |
| !! | TCCACTTTCAAAAAAAAAAA+AGG | - | chr4.4:21483122-21483141 | MS.gene053590:intron | 20.0% |
| !!! | AAAAATGAAAGGTTTGAAAT+GGG | + | chr4.4:21483006-21483025 | None:intergenic | 20.0% |
| !!! | ATTTTTTTTAATCATAGCAC+AGG | - | chr4.4:21484118-21484137 | MS.gene053590:intron | 20.0% |
| !!! | GAATTTCTAAGGTATTTTTT+TGG | + | chr4.4:21483356-21483375 | None:intergenic | 20.0% |
| !!! | TCCTTTTTTTTTTTTGAAAG+TGG | + | chr4.4:21483126-21483145 | None:intergenic | 20.0% |
| !!! | TGTTAATTTGATTAGATACT+TGG | - | chr4.4:21483883-21483902 | MS.gene053590:intron | 20.0% |
| !!! | TTTTTTTAGAGGAAAAATAG+GGG | - | chr4.4:21483199-21483218 | MS.gene053590:CDS | 20.0% |
| ! | ATTGCTAATTTAGTAAGAGT+TGG | - | chr4.4:21484436-21484455 | MS.gene053590:CDS | 25.0% |
| ! | GAAGTAATAAAGGAAAATGA+TGG | + | chr4.4:21484370-21484389 | None:intergenic | 25.0% |
| ! | GAATATGTGAAGAAAATGTA+CGG | - | chr4.4:21482662-21482681 | MS.gene053590:CDS | 25.0% |
| ! | GTAGAAAGGAAAAAAATGAA+AGG | + | chr4.4:21483017-21483036 | None:intergenic | 25.0% |
| ! | TACAAAAAAAGACAAAACCA+TGG | + | chr4.4:21483740-21483759 | None:intergenic | 25.0% |
| !! | ATATAAGTGCTTTATTACCA+TGG | - | chr4.4:21483720-21483739 | MS.gene053590:intron | 25.0% |
| !! | TCTTGATATATTTCTCCTTT+TGG | - | chr4.4:21484156-21484175 | MS.gene053590:intron | 25.0% |
| !!! | AAATTTTGCTATGAGATTGA+AGG | - | chr4.4:21484478-21484497 | MS.gene053590:CDS | 25.0% |
| !!! | CAAAATTTTAAAATAGTCCC+TGG | + | chr4.4:21483291-21483310 | None:intergenic | 25.0% |
| !!! | GGGAAATAGTTTTTTTTTAG+AGG | - | chr4.4:21483188-21483207 | MS.gene053590:CDS | 25.0% |
| !!! | TTTTGGGTAATAGTCAATTT+GGG | + | chr4.4:21483339-21483358 | None:intergenic | 25.0% |
| !!! | TTTTTGGGTAATAGTCAATT+TGG | + | chr4.4:21483340-21483359 | None:intergenic | 25.0% |
| AATAGGGATAAATACTGATG+TGG | - | chr4.4:21483145-21483164 | MS.gene053590:intron | 30.0% | |
| AGATATGGAGATCACATTAT+GGG | - | chr4.4:21484259-21484278 | MS.gene053590:intron | 30.0% | |
| AGATTCAATTCATCATCCTT+TGG | + | chr4.4:21482770-21482789 | None:intergenic | 30.0% | |
| AGTGGTTATTGTGATCATTA+AGG | - | chr4.4:21483223-21483242 | MS.gene053590:CDS | 30.0% | |
| CACATACTAACTAACAATAG+GGG | - | chr4.4:21483168-21483187 | MS.gene053590:intron | 30.0% | |
| CTTCTAATTCAGCTTTACAT+TGG | + | chr4.4:21483773-21483792 | None:intergenic | 30.0% | |
| GATGAATTGAATCTGTATGA+TGG | - | chr4.4:21482776-21482795 | MS.gene053590:intron | 30.0% | |
| GCACATACTAACTAACAATA+GGG | - | chr4.4:21483167-21483186 | MS.gene053590:intron | 30.0% | |
| GGAATTGAGATGTTTAGATA+TGG | - | chr4.4:21484244-21484263 | MS.gene053590:intron | 30.0% | |
| GTGGTTATTGTGATCATTAA+GGG | - | chr4.4:21483224-21483243 | MS.gene053590:CDS | 30.0% | |
| TAGATATGGAGATCACATTA+TGG | - | chr4.4:21484258-21484277 | MS.gene053590:intron | 30.0% | |
| TATTCAGCGAAATTTCATCA+CGG | - | chr4.4:21484339-21484358 | MS.gene053590:CDS | 30.0% | |
| TGAAATTTCGCTGAATAAGA+CGG | + | chr4.4:21484336-21484355 | None:intergenic | 30.0% | |
| ! | AGGGTGATAATGTTATGTTT+CGG | + | chr4.4:21483963-21483982 | None:intergenic | 30.0% |
| ! | TCATTATCTACGTTTTGTCA+AGG | + | chr4.4:21483471-21483490 | None:intergenic | 30.0% |
| !!! | GACTATTTTGACTAACAAAG+TGG | - | chr4.4:21483264-21483283 | MS.gene053590:CDS | 30.0% |
| !!! | TTATGATTTTTGGAGTTGTC+TGG | + | chr4.4:21483545-21483564 | None:intergenic | 30.0% |
| AAAATCCTTGGCATTTGCTT+TGG | - | chr4.4:21482896-21482915 | MS.gene053590:CDS | 35.0% | |
| ACCAGTCCTAAGAATTTCTA+AGG | + | chr4.4:21483367-21483386 | None:intergenic | 35.0% | |
| ACCTTAGAAATTCTTAGGAC+TGG | - | chr4.4:21483363-21483382 | MS.gene053590:intron | 35.0% | |
| ATGCACATGGAAATGATACA+TGG | - | chr4.4:21482825-21482844 | MS.gene053590:intron | 35.0% | |
| ATGTGAAGAAAATGTACGGA+GGG | - | chr4.4:21482666-21482685 | MS.gene053590:CDS | 35.0% | |
| ATGTGAAGATTGCATCACAT+GGG | + | chr4.4:21483658-21483677 | None:intergenic | 35.0% | |
| ATTGCTAAATCGGATAAGAC+TGG | - | chr4.4:21484223-21484242 | MS.gene053590:intron | 35.0% | |
| CACACTTGTCTATATAAGCA+TGG | - | chr4.4:21483632-21483651 | MS.gene053590:intron | 35.0% | |
| CTTTACGTTACGACATGATA+TGG | - | chr4.4:21483684-21483703 | MS.gene053590:intron | 35.0% | |
| GACATGAACAAGAAAATCCT+TGG | - | chr4.4:21482884-21482903 | MS.gene053590:CDS | 35.0% | |
| GATCACATTATGGGAATTCA+AGG | - | chr4.4:21484268-21484287 | MS.gene053590:intron | 35.0% | |
| GATTTACATGTCGCAAAAAC+TGG | + | chr4.4:21483601-21483620 | None:intergenic | 35.0% | |
| GGCACATACTAACTAACAAT+AGG | - | chr4.4:21483166-21483185 | MS.gene053590:intron | 35.0% | |
| GGTCGTTATTGTGAAAGAAA+TGG | - | chr4.4:21484566-21484585 | MS.gene053590:CDS | 35.0% | |
| TAGTGCGTTAAATATCCCAA+AGG | + | chr4.4:21483397-21483416 | None:intergenic | 35.0% | |
| TATGTGAAGAAAATGTACGG+AGG | - | chr4.4:21482665-21482684 | MS.gene053590:CDS | 35.0% | |
| TGCACATGGAAATGATACAT+GGG | - | chr4.4:21482826-21482845 | MS.gene053590:intron | 35.0% | |
| TTCCATTATTCAATGCCACA+GGG | - | chr4.4:21484026-21484045 | MS.gene053590:intron | 35.0% | |
| TTTCCATTATTCAATGCCAC+AGG | - | chr4.4:21484025-21484044 | MS.gene053590:intron | 35.0% | |
| ! | TGAATAATGGAAAGACTCGT+TGG | + | chr4.4:21484018-21484037 | None:intergenic | 35.0% |
| !! | AGAATGTCTTTAGAGTACTC+AGG | + | chr4.4:21484298-21484317 | None:intergenic | 35.0% |
| !! | GTTGGTTGATTTGAATTGAC+AGG | - | chr4.4:21484454-21484473 | MS.gene053590:CDS | 35.0% |
| !!! | AAAATGTACGGAGGGTATTT+TGG | - | chr4.4:21482674-21482693 | MS.gene053590:CDS | 35.0% |
| !!! | AAATGTACGGAGGGTATTTT+GGG | - | chr4.4:21482675-21482694 | MS.gene053590:CDS | 35.0% |
| !!! | TATGATGGTTTTGTGATCAC+TGG | - | chr4.4:21482791-21482810 | MS.gene053590:intron | 35.0% |
| CAGCTTTACATTGGCATCAT+AGG | + | chr4.4:21483764-21483783 | None:intergenic | 40.0% | |
| CATGATGAGCGTATAACACA+TGG | - | chr4.4:21483090-21483109 | MS.gene053590:intron | 40.0% | |
| CATGTGAAGATTGCATCACA+TGG | + | chr4.4:21483659-21483678 | None:intergenic | 40.0% | |
| GGAAGTTCAAGAGAAGACAA+AGG | + | chr4.4:21483997-21484016 | None:intergenic | 40.0% | |
| GGCTGAAGTGATTGCTAAAT+CGG | - | chr4.4:21484213-21484232 | MS.gene053590:intron | 40.0% | |
| GGTATTTGTGAGAATGTTGG+AGG | - | chr4.4:21482697-21482716 | MS.gene053590:CDS | 40.0% | |
| TAGAGGAAAAATAGGGGAAG+TGG | - | chr4.4:21483205-21483224 | MS.gene053590:CDS | 40.0% | |
| TCAAGAGAAGACAAAGGAGA+TGG | + | chr4.4:21483991-21484010 | None:intergenic | 40.0% | |
| TGAAAGCTGGGATGTGTATA+AGG | - | chr4.4:21482727-21482746 | MS.gene053590:CDS | 40.0% | |
| TGCTATGAGATTGAAGGAGA+AGG | - | chr4.4:21484484-21484503 | MS.gene053590:CDS | 40.0% | |
| TTGACTAACAAAGTGGACCA+GGG | - | chr4.4:21483271-21483290 | MS.gene053590:CDS | 40.0% | |
| TTTAGCAATCACTTCAGCCT+TGG | + | chr4.4:21484212-21484231 | None:intergenic | 40.0% | |
| TTTCACAATAACGACCCTTG+AGG | + | chr4.4:21484562-21484581 | None:intergenic | 40.0% | |
| TTTGACTAACAAAGTGGACC+AGG | - | chr4.4:21483270-21483289 | MS.gene053590:CDS | 40.0% | |
| ! | ATCCCTGTGGCATTGAATAA+TGG | + | chr4.4:21484031-21484050 | None:intergenic | 40.0% |
| ! | TATCTACGTTTTGTCAAGGC+AGG | + | chr4.4:21483467-21483486 | None:intergenic | 40.0% |
| ! | TGGGGTATTTGTGAGAATGT+TGG | - | chr4.4:21482694-21482713 | MS.gene053590:CDS | 40.0% |
| !!! | AATGTACGGAGGGTATTTTG+GGG | - | chr4.4:21482676-21482695 | MS.gene053590:CDS | 40.0% |
| !!! | TTTTTTGAAAGTGGACCACC+TGG | + | chr4.4:21483117-21483136 | None:intergenic | 40.0% |
| AAGCTGGGATGTGTATAAGG+TGG | - | chr4.4:21482730-21482749 | MS.gene053590:intron | 45.0% | |
| ACTATGCATCAGCTTCCTCA+AGG | - | chr4.4:21484544-21484563 | MS.gene053590:CDS | 45.0% | |
| AGACAAAGGAGATGGCAAAG+AGG | + | chr4.4:21483983-21484002 | None:intergenic | 45.0% | |
| ATGAGATTGAAGGAGAAGGC+TGG | - | chr4.4:21484488-21484507 | MS.gene053590:CDS | 45.0% | |
| ATGGAGAAAATGGTAGGGAG+TGG | - | chr4.4:21482602-21482621 | MS.gene053590:CDS | 45.0% | |
| CTATGCATCAGCTTCCTCAA+GGG | - | chr4.4:21484545-21484564 | MS.gene053590:CDS | 45.0% | |
| GACAAAGGAGATGGCAAAGA+GGG | + | chr4.4:21483982-21484001 | None:intergenic | 45.0% | |
| GATGACCAAAGCAAATGCCA+AGG | + | chr4.4:21482904-21482923 | None:intergenic | 45.0% | |
| GGTAGTTGTAGTGATGCACA+TGG | - | chr4.4:21482812-21482831 | MS.gene053590:intron | 45.0% | |
| GTGAGAATGTTGGAGGAAGA+AGG | - | chr4.4:21482704-21482723 | MS.gene053590:CDS | 45.0% | |
| TAGATACTTGGCCGTGCAAT+TGG | - | chr4.4:21483895-21483914 | MS.gene053590:intron | 45.0% | |
| TAGGGAGTGGGAAGAGATTT+GGG | - | chr4.4:21482615-21482634 | MS.gene053590:CDS | 45.0% | |
| TATTCAATGCCACAGGGATG+AGG | - | chr4.4:21484032-21484051 | MS.gene053590:intron | 45.0% | |
| TGGAGAAAATGGTAGGGAGT+GGG | - | chr4.4:21482603-21482622 | MS.gene053590:CDS | 45.0% | |
| TGGCATTTGCTTTGGTCATC+AGG | - | chr4.4:21482904-21482923 | MS.gene053590:CDS | 45.0% | |
| AAGGTGACTCGATCTCCAAC+TGG | - | chr4.4:21483922-21483941 | MS.gene053590:intron | 50.0% | |
| ACACCAATGTCCCATCCAGT+TGG | + | chr4.4:21483940-21483959 | None:intergenic | 50.0% | |
| AGGGAGTGGGAAGAGATTTG+GGG | - | chr4.4:21482616-21482635 | MS.gene053590:CDS | 50.0% | |
| ATACTTGGCCGTGCAATTGG+AGG | - | chr4.4:21483898-21483917 | MS.gene053590:intron | 50.0% | |
| CGTATAACACATGGTCCAGG+TGG | - | chr4.4:21483099-21483118 | MS.gene053590:intron | 50.0% | |
| CTCTTCCATGCCTCCTTATC+TGG | + | chr4.4:21484524-21484543 | None:intergenic | 50.0% | |
| GAAGGAGAAGGCTGGTATGT+GGG | - | chr4.4:21484496-21484515 | MS.gene053590:CDS | 50.0% | |
| GACTCGATCTCCAACTGGAT+GGG | - | chr4.4:21483927-21483946 | MS.gene053590:intron | 50.0% | |
| GAGCGTATAACACATGGTCC+AGG | - | chr4.4:21483096-21483115 | MS.gene053590:intron | 50.0% | |
| GGAGGAAGAAGGTGAAAGCT+GGG | - | chr4.4:21482715-21482734 | MS.gene053590:CDS | 50.0% | |
| GTAGGGAGTGGGAAGAGATT+TGG | - | chr4.4:21482614-21482633 | MS.gene053590:CDS | 50.0% | |
| TACTTGGCCGTGCAATTGGA+GGG | - | chr4.4:21483899-21483918 | MS.gene053590:intron | 50.0% | |
| TATGTGGGAGCCAGATAAGG+AGG | - | chr4.4:21484511-21484530 | MS.gene053590:CDS | 50.0% | |
| TCACCTTCCCTCCAATTGCA+CGG | + | chr4.4:21483909-21483928 | None:intergenic | 50.0% | |
| TCTCCAACTGGATGGGACAT+TGG | - | chr4.4:21483934-21483953 | MS.gene053590:intron | 50.0% | |
| TGAAGGAGAAGGCTGGTATG+TGG | - | chr4.4:21484495-21484514 | MS.gene053590:CDS | 50.0% | |
| TGACTCGATCTCCAACTGGA+TGG | - | chr4.4:21483926-21483945 | MS.gene053590:intron | 50.0% | |
| TGGAGGAAGAAGGTGAAAGC+TGG | - | chr4.4:21482714-21482733 | MS.gene053590:CDS | 50.0% | |
| TGGTATGTGGGAGCCAGATA+AGG | - | chr4.4:21484508-21484527 | MS.gene053590:CDS | 50.0% | |
| !! | AGGACTGGTTCTCTCTCCTT+TGG | - | chr4.4:21483378-21483397 | MS.gene053590:intron | 50.0% |
| !! | GGACTGGTTCTCTCTCCTTT+GGG | - | chr4.4:21483379-21483398 | MS.gene053590:intron | 50.0% |
| ACACACACACCTCATCCCTG+TGG | + | chr4.4:21484044-21484063 | None:intergenic | 55.0% | |
| GATGTGTATAAGGTGGCGCG+TGG | - | chr4.4:21482737-21482756 | MS.gene053590:intron | 55.0% | |
| GCAATCACTTCAGCCTTGGC+AGG | + | chr4.4:21484208-21484227 | None:intergenic | 55.0% | |
| GCTAAGAGAGCTACCTGCCA+AGG | - | chr4.4:21484192-21484211 | MS.gene053590:intron | 55.0% | |
| GGCGCGTGGAGAGTTTCCAA+AGG | - | chr4.4:21482751-21482770 | MS.gene053590:intron | 60.0% | |
| GGGAGCCAGATAAGGAGGCA+TGG | - | chr4.4:21484516-21484535 | MS.gene053590:CDS | 60.0% | |
| TGGCCGTGCAATTGGAGGGA+AGG | - | chr4.4:21483903-21483922 | MS.gene053590:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 21482602 | 21484622 | 21482602 | ID=MS.gene053590 |
| chr4.4 | mRNA | 21482602 | 21484622 | 21482602 | ID=MS.gene053590.t1;Parent=MS.gene053590 |
| chr4.4 | exon | 21484299 | 21484622 | 21484299 | ID=MS.gene053590.t1.exon1;Parent=MS.gene053590.t1 |
| chr4.4 | CDS | 21484299 | 21484622 | 21484299 | ID=cds.MS.gene053590.t1;Parent=MS.gene053590.t1 |
| chr4.4 | exon | 21483171 | 21483326 | 21483171 | ID=MS.gene053590.t1.exon2;Parent=MS.gene053590.t1 |
| chr4.4 | CDS | 21483171 | 21483326 | 21483171 | ID=cds.MS.gene053590.t1;Parent=MS.gene053590.t1 |
| chr4.4 | exon | 21482864 | 21483031 | 21482864 | ID=MS.gene053590.t1.exon3;Parent=MS.gene053590.t1 |
| chr4.4 | CDS | 21482864 | 21483031 | 21482864 | ID=cds.MS.gene053590.t1;Parent=MS.gene053590.t1 |
| chr4.4 | exon | 21482602 | 21482748 | 21482602 | ID=MS.gene053590.t1.exon4;Parent=MS.gene053590.t1 |
| chr4.4 | CDS | 21482602 | 21482748 | 21482602 | ID=cds.MS.gene053590.t1;Parent=MS.gene053590.t1 |
| Gene Sequence |
| Protein sequence |
