Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07348.t1 | RHN80988.1 | 100 | 57 | 0 | 0 | 1 | 57 | 6 | 62 | 7.90E-23 | 115.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07348.t1 | G7IBT4 | 100.0 | 57 | 0 | 0 | 1 | 57 | 168 | 224 | 5.7e-23 | 115.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene07348 | -0.811344 | 7.34E-51 | -1.69E-46 |
| MS.gene050945 | MS.gene07348 | -0.83565 | 1.48E-56 | -1.69E-46 |
| MS.gene051960 | MS.gene07348 | -0.81053 | 1.10E-50 | -1.69E-46 |
| MS.gene053083 | MS.gene07348 | -0.803146 | 4.01E-49 | -1.69E-46 |
| MS.gene054413 | MS.gene07348 | -0.838499 | 2.77E-57 | -1.69E-46 |
| MS.gene055765 | MS.gene07348 | -0.82656 | 2.54E-54 | -1.69E-46 |
| MS.gene055849 | MS.gene07348 | 0.802377 | 5.77E-49 | -1.69E-46 |
| MS.gene057684 | MS.gene07348 | 0.841693 | 4.07E-58 | -1.69E-46 |
| MS.gene057687 | MS.gene07348 | 0.805395 | 1.36E-49 | -1.69E-46 |
| MS.gene059837 | MS.gene07348 | 0.842235 | 2.93E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATCAATGTAAAATAAAAT+AGG | + | 8593:41493-41512 | None:intergenic | 15.0% |
| !!! | AAAACATCACATTTTTATAA+AGG | + | 8593:41964-41983 | None:intergenic | 15.0% |
| !!! | ATATATTGATAGTATTGATA+AGG | - | 8593:42324-42343 | MS.gene07348:intron | 15.0% |
| !!! | TATATTGATAGTATTGATAA+GGG | - | 8593:42325-42344 | MS.gene07348:intron | 15.0% |
| !! | CTTTCCTAATTTATTTGTTT+GGG | - | 8593:42072-42091 | MS.gene07348:intron | 20.0% |
| !! | TGTAAACGATCTTATATTTA+GGG | - | 8593:42423-42442 | MS.gene07348:intron | 20.0% |
| !! | TTAACTTCTAACATGAAATT+AGG | - | 8593:41763-41782 | MS.gene07348:intron | 20.0% |
| !! | TTGTAAACGATCTTATATTT+AGG | - | 8593:42422-42441 | MS.gene07348:intron | 20.0% |
| !!! | AACCAAAACTTGATATTTTT+TGG | + | 8593:42522-42541 | None:intergenic | 20.0% |
| !!! | ATACCCAATTTTTCATTATT+TGG | - | 8593:42017-42036 | MS.gene07348:intron | 20.0% |
| !!! | ATATTGATAGTATTGATAAG+GGG | - | 8593:42326-42345 | MS.gene07348:intron | 20.0% |
| !!! | TACCCAATTTTTCATTATTT+GGG | - | 8593:42018-42037 | MS.gene07348:intron | 20.0% |
| !!! | TGTAGATAGTCTTATATTTA+AGG | - | 8593:42387-42406 | MS.gene07348:intron | 20.0% |
| !!! | TTCCAAAAAATATCAAGTTT+TGG | - | 8593:42517-42536 | MS.gene07348:intron | 20.0% |
| ! | AAAAATTGGGTATTCTTTCA+CGG | + | 8593:42010-42029 | None:intergenic | 25.0% |
| ! | AGAACCCAAACAAATAAATT+AGG | + | 8593:42079-42098 | None:intergenic | 25.0% |
| ! | CATATTTAGGGGAAAAAATT+GGG | - | 8593:41560-41579 | MS.gene07348:intron | 25.0% |
| ! | GCTTTCCTAATTTATTTGTT+TGG | - | 8593:42071-42090 | MS.gene07348:intron | 25.0% |
| ! | TAGAATGAGAAACTTACAAT+TGG | + | 8593:41595-41614 | None:intergenic | 25.0% |
| ! | TCCCCAAATAATGAAAAATT+GGG | + | 8593:42023-42042 | None:intergenic | 25.0% |
| ! | TTAAGTAAACCTTCAAGAAA+TGG | + | 8593:42712-42731 | None:intergenic | 25.0% |
| ! | TTACTAACTACTCAATTTGA+AGG | - | 8593:42550-42569 | MS.gene07348:intron | 25.0% |
| ! | TTACTTTGATGCCATATTTA+GGG | - | 8593:41548-41567 | MS.gene07348:intron | 25.0% |
| ! | TTTGTACACAAATGACAATT+TGG | + | 8593:42273-42292 | None:intergenic | 25.0% |
| !! | AATTTTTCATTATTTGGGGA+CGG | - | 8593:42023-42042 | MS.gene07348:intron | 25.0% |
| !! | ACCCAATTTTTCATTATTTG+GGG | - | 8593:42019-42038 | MS.gene07348:intron | 25.0% |
| !!! | AGACTAGTTTACAAAAATCA+CGG | - | 8593:42196-42215 | MS.gene07348:intron | 25.0% |
| !!! | GTGATGTTTTTTACAACTTT+AGG | - | 8593:41974-41993 | MS.gene07348:intron | 25.0% |
| AACGATCTTATATTTAGGGA+TGG | - | 8593:42427-42446 | MS.gene07348:intron | 30.0% | |
| ATCTTATATTTAGGGATGGA+GGG | - | 8593:42431-42450 | MS.gene07348:intron | 30.0% | |
| CCATATTTAGGGGAAAAAAT+TGG | - | 8593:41559-41578 | MS.gene07348:intron | 30.0% | |
| CGGATAAAAAGTAAGAAAAG+TGG | - | 8593:42043-42062 | MS.gene07348:intron | 30.0% | |
| CTAAATGAACAACTCACTTT+AGG | - | 8593:42106-42125 | MS.gene07348:intron | 30.0% | |
| CTTACTTTGATGCCATATTT+AGG | - | 8593:41547-41566 | MS.gene07348:intron | 30.0% | |
| GAGAAACTTACAATTGGTAT+TGG | + | 8593:41589-41608 | None:intergenic | 30.0% | |
| GTCCCCAAATAATGAAAAAT+TGG | + | 8593:42024-42043 | None:intergenic | 30.0% | |
| TAAAATGCCTTACTTCTACA+AGG | + | 8593:41450-41469 | None:intergenic | 30.0% | |
| TACTTTGATGCCATATTTAG+GGG | - | 8593:41549-41568 | MS.gene07348:intron | 30.0% | |
| TGAAAAGTGAGATTAACGAA+TGG | + | 8593:42584-42603 | None:intergenic | 30.0% | |
| TTGCAAAAAGCTACAAATGA+AGG | - | 8593:42758-42777 | MS.gene07348:CDS | 30.0% | |
| ! | ATGTTAGAAGTTAAAAGCGT+GGG | + | 8593:41757-41776 | None:intergenic | 30.0% |
| !!! | CCAATTTTTTCCCCTAAATA+TGG | + | 8593:41562-41581 | None:intergenic | 30.0% |
| AGATAAGAGTCCATTGTAAC+TGG | + | 8593:42607-42626 | None:intergenic | 35.0% | |
| AGGTTTACTTAAACGTGGTT+TGG | - | 8593:42720-42739 | MS.gene07348:intron | 35.0% | |
| CATGTTAGAAGTTAAAAGCG+TGG | + | 8593:41758-41777 | None:intergenic | 35.0% | |
| CTTGAAGGTTTACTTAAACG+TGG | - | 8593:42715-42734 | MS.gene07348:intron | 35.0% | |
| GATCTTATATTTAGGGATGG+AGG | - | 8593:42430-42449 | MS.gene07348:intron | 35.0% | |
| TGCTTGACTAAAAGCTGAAT+GGG | + | 8593:41842-41861 | None:intergenic | 35.0% | |
| ! | AGTCTTGTAAAACGAACGAA+GGG | + | 8593:42181-42200 | None:intergenic | 35.0% |
| ! | TTTGTAGCTTTTTGCAAACG+TGG | + | 8593:42755-42774 | None:intergenic | 35.0% |
| !! | TAGTCTTGTAAAACGAACGA+AGG | + | 8593:42182-42201 | None:intergenic | 35.0% |
| !! | TCACTTTTCACCAGTTACAA+TGG | - | 8593:42594-42613 | MS.gene07348:intron | 35.0% |
| AACTAGCTGCAAGTTCCTAT+TGG | - | 8593:41625-41644 | MS.gene07348:intron | 40.0% | |
| ACTAGCTGCAAGTTCCTATT+GGG | - | 8593:41626-41645 | MS.gene07348:intron | 40.0% | |
| ACTTCTACAAGGCATGAAGA+AGG | + | 8593:41439-41458 | None:intergenic | 40.0% | |
| CTTCATGCCTTGTAGAAGTA+AGG | - | 8593:41440-41459 | MS.gene07348:CDS | 40.0% | |
| GAGACTGTCAGCTGTAATAA+TGG | + | 8593:41891-41910 | None:intergenic | 40.0% | |
| GCTTGACTAAAAGCTGAATG+GGG | + | 8593:41841-41860 | None:intergenic | 40.0% | |
| GGAGAGGAGGAAAATACAAA+GGG | + | 8593:42664-42683 | None:intergenic | 40.0% | |
| GTGCTTGACTAAAAGCTGAA+TGG | + | 8593:41843-41862 | None:intergenic | 40.0% | |
| TGGAGAGGAGGAAAATACAA+AGG | + | 8593:42665-42684 | None:intergenic | 40.0% | |
| TTCCTTAGTGTGCATACATG+TGG | + | 8593:41701-41720 | None:intergenic | 40.0% | |
| ! | TTGTATTTTCCTCCTCTCCA+TGG | - | 8593:42665-42684 | MS.gene07348:intron | 40.0% |
| !! | GAACGAAGGGAGTATTGATT+TGG | + | 8593:42168-42187 | None:intergenic | 40.0% |
| ACAGCTGACAGTCTCATATG+AGG | - | 8593:41896-41915 | MS.gene07348:intron | 45.0% | |
| ACTAAAAGCTGAATGGGGAC+AGG | + | 8593:41836-41855 | None:intergenic | 45.0% | |
| AGTGCCTGCAGATTATACCA+TGG | + | 8593:42685-42704 | None:intergenic | 45.0% | |
| CACCACATGTATGCACACTA+AGG | - | 8593:41696-41715 | MS.gene07348:intron | 45.0% | |
| CAGATTATACCATGGAGAGG+AGG | + | 8593:42677-42696 | None:intergenic | 45.0% | |
| CTAAAAGCTGAATGGGGACA+GGG | + | 8593:41835-41854 | None:intergenic | 45.0% | |
| CTCTCCATGGTATAATCTGC+AGG | - | 8593:42678-42697 | MS.gene07348:intron | 45.0% | |
| CTGCAGATTATACCATGGAG+AGG | + | 8593:42680-42699 | None:intergenic | 45.0% | |
| GCACTGCAACCATTTCTTGA+AGG | - | 8593:42700-42719 | MS.gene07348:intron | 45.0% | |
| GCTGTGCGATTCTATCCAAA+AGG | - | 8593:41412-41431 | MS.gene07348:CDS | 45.0% | |
| TAAAAGCTGAATGGGGACAG+GGG | + | 8593:41834-41853 | None:intergenic | 45.0% | |
| ! | AGGCATGAAGAAGGACCTTT+TGG | + | 8593:41430-41449 | None:intergenic | 45.0% |
| !!! | ACTTTTAGCTCCAGTGGCAT+CGG | - | 8593:41926-41945 | MS.gene07348:intron | 45.0% |
| !!! | TCCTCAACTTTTAGCTCCAG+TGG | - | 8593:41920-41939 | MS.gene07348:intron | 45.0% |
| AGGCAATTAGGTGGCCCAAT+AGG | + | 8593:41643-41662 | None:intergenic | 50.0% | |
| CCTAATTGCCTGTCCCTATC+AGG | - | 8593:41652-41671 | MS.gene07348:intron | 50.0% | |
| CCTGATAGGGACAGGCAATT+AGG | + | 8593:41655-41674 | None:intergenic | 50.0% | |
| GATAGGGACAGGCAATTAGG+TGG | + | 8593:41652-41671 | None:intergenic | 50.0% | |
| GCCACTGGAGCTAAAAGTTG+AGG | + | 8593:41924-41943 | None:intergenic | 50.0% | |
| TATCAGGCCAGTGCATGACT+AGG | - | 8593:41668-41687 | MS.gene07348:intron | 50.0% | |
| ! | AGAGTACTTACCGATGCCAC+TGG | + | 8593:41939-41958 | None:intergenic | 50.0% |
| ! | AGTCATGCACTGGCCTGATA+GGG | + | 8593:41668-41687 | None:intergenic | 50.0% |
| ! | TAGTCATGCACTGGCCTGAT+AGG | + | 8593:41669-41688 | None:intergenic | 50.0% |
| !! | AGTGGCATCGGTAAGTACTC+TGG | - | 8593:41938-41957 | MS.gene07348:intron | 50.0% |
| TGTGCCACCTAGTCATGCAC+TGG | + | 8593:41678-41697 | None:intergenic | 55.0% | |
| AAGCTGAATGGGGACAGGGG+AGG | + | 8593:41831-41850 | None:intergenic | 60.0% | |
| CAGGCCAGTGCATGACTAGG+TGG | - | 8593:41671-41690 | MS.gene07348:intron | 60.0% | |
| GGGGACAGGGGAGGAGAAAA+TGG | + | 8593:41822-41841 | None:intergenic | 60.0% | |
| ! | TGCACTGGCCTGATAGGGAC+AGG | + | 8593:41663-41682 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8593 | gene | 41408 | 42777 | 41408 | ID=MS.gene07348 |
| 8593 | mRNA | 41408 | 42777 | 41408 | ID=MS.gene07348.t1;Parent=MS.gene07348 |
| 8593 | exon | 42729 | 42777 | 42729 | ID=MS.gene07348.t1.exon1;Parent=MS.gene07348.t1 |
| 8593 | CDS | 42729 | 42777 | 42729 | ID=cds.MS.gene07348.t1;Parent=MS.gene07348.t1 |
| 8593 | exon | 42238 | 42285 | 42238 | ID=MS.gene07348.t1.exon2;Parent=MS.gene07348.t1 |
| 8593 | CDS | 42238 | 42285 | 42238 | ID=cds.MS.gene07348.t1;Parent=MS.gene07348.t1 |
| 8593 | exon | 41408 | 41485 | 41408 | ID=MS.gene07348.t1.exon3;Parent=MS.gene07348.t1 |
| 8593 | CDS | 41408 | 41485 | 41408 | ID=cds.MS.gene07348.t1;Parent=MS.gene07348.t1 |
| Gene Sequence |
| Protein sequence |
