AlfalfaGEDB | MS.gene073851

M. sativa cultivar XinJiangDaYe / MS.gene073851

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073851.t1	XP_003590511.1	100	129	0	0	1	129	1	129	3.80E-41	177.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073851.t1	O22582	100.0	90	0	0	40	129	51	140	1.0e-42	174.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073851.t1	G7IBM3	100.0	129	0	0	1	129	1	129	2.7e-41	177.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050689	MS.gene073851	0.804369	2.23E-49	-1.69E-46
MS.gene050901	MS.gene073851	0.805265	1.45E-49	-1.69E-46
MS.gene053122	MS.gene073851	0.806551	7.79E-50	-1.69E-46
MS.gene053226	MS.gene073851	0.817633	2.98E-52	-1.69E-46
MS.gene05364	MS.gene073851	0.815339	9.72E-52	-1.69E-46
MS.gene053931	MS.gene073851	0.803095	4.11E-49	-1.69E-46
MS.gene055694	MS.gene073851	0.802436	5.61E-49	-1.69E-46
MS.gene056215	MS.gene073851	0.814895	1.22E-51	-1.69E-46
MS.gene057034	MS.gene073851	0.814002	1.92E-51	-1.69E-46
MS.gene057208	MS.gene073851	0.811525	6.70E-51	-1.69E-46
MS.gene057444	MS.gene073851	0.803036	4.22E-49	-1.69E-46
MS.gene058976	MS.gene073851	0.930273	2.08E-93	-1.69E-46
MS.gene058979	MS.gene073851	0.800426	1.45E-48	-1.69E-46
MS.gene060429	MS.gene073851	0.810037	1.41E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073851.t1	MTR_1g068600	99.225	129	1	0	1	129	1	129	1.61e-88	253
MS.gene073851.t1	MTR_4g063240	84.286	140	11	2	1	129	1	140	3.35e-76	223
MS.gene073851.t1	MTR_4g063220	85.106	141	9	2	1	129	1	141	5.55e-73	214
MS.gene073851.t1	MTR_4g063990	85.106	141	9	2	1	129	1	141	5.55e-73	214
MS.gene073851.t1	MTR_4g063200	85.106	141	9	2	1	129	1	141	5.55e-73	214
MS.gene073851.t1	MTR_4g064020	85.106	141	9	2	1	129	1	141	5.55e-73	214
MS.gene073851.t1	MTR_1g090780	90.840	131	9	2	1	129	1	130	7.30e-73	214
MS.gene073851.t1	MTR_1g492910	90.840	131	9	2	1	129	1	130	7.54e-73	214
MS.gene073851.t1	MTR_4g071180	85.106	141	9	2	1	129	1	141	7.88e-72	211
MS.gene073851.t1	MTR_4g070240	85.106	141	9	2	1	129	1	141	7.88e-72	211
MS.gene073851.t1	MTR_4g064983	84.397	141	10	2	1	129	1	141	4.64e-71	213
MS.gene073851.t1	MTR_4g088025	86.029	136	12	2	1	129	1	136	1.47e-70	208
MS.gene073851.t1	MTR_4g070245	83.688	141	11	2	1	129	1	141	2.72e-70	207
MS.gene073851.t1	MTR_4g063275	82.270	141	13	2	1	129	1	141	7.54e-70	206
MS.gene073851.t1	MTR_7g099960	95.050	101	4	1	30	129	31	131	2.32e-59	179
MS.gene073851.t1	MTR_8g069990	71.591	88	23	2	8	95	3	88	4.70e-35	117
MS.gene073851.t1	MTR_2g084480	63.855	83	30	0	47	129	136	218	4.93e-35	120
MS.gene073851.t1	MTR_4g063270	90.196	51	5	0	74	124	1	51	3.72e-27	95.5
MS.gene073851.t1	MTR_7g114580	88.235	34	4	0	96	129	11	44	3.85e-15	65.1
MS.gene073851.t1	MTR_4g032777	39.744	78	47	0	43	120	33	110	1.24e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073851.t1	AT3G45980	82.517	143	11	2	1	129	1	143	1.16e-71	211
MS.gene073851.t1	AT2G37470	87.879	132	13	2	1	129	1	132	3.63e-71	209
MS.gene073851.t1	AT1G07790	82.979	141	12	2	1	129	1	141	4.61e-70	207
MS.gene073851.t1	AT2G28720	82.639	144	10	2	1	129	1	144	2.69e-69	205
MS.gene073851.t1	AT3G53650	87.023	131	15	2	1	129	1	131	2.56e-68	202
MS.gene073851.t1	AT5G59910	86.719	128	14	1	5	129	16	143	7.71e-68	201
MS.gene073851.t1	AT3G46030	87.705	122	12	1	11	129	17	138	2.66e-66	197
MS.gene073851.t1	AT5G02570	82.707	133	11	2	1	129	1	125	2.35e-63	189
MS.gene073851.t1	AT3G09480	79.070	129	17	1	1	129	1	119	1.27e-60	182
MS.gene073851.t1	AT5G22880	80.147	136	17	2	4	129	3	138	2.91e-60	182
MS.gene073851.t1	AT1G08170	62.963	81	30	0	49	129	151	231	9.47e-36	123

Find 31 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGAAGATGTAAATCTTAT+AGG	0.133504	1.3:-47861573	None:intergenic
TTCTTTCGAGATCTTCTTCT+CGG	0.293193	1.3:-47861507	None:intergenic
GCTGTGCGTTTGGTTCTTCC+TGG	0.321985	1.3:+47861755	MS.gene073851:CDS
TTTGAAGAAATACCTATATC+AGG	0.334896	1.3:-47861614	None:intergenic
CATCTTCAAGGTTCTCAAAC+AGG	0.361477	1.3:+47861586	MS.gene073851:CDS
GAGATCTTCTTCTCGGCTCT+TGG	0.362586	1.3:-47861500	None:intergenic
CTATAAGATTTACATCTTCA+AGG	0.370441	1.3:+47861574	MS.gene073851:CDS
GATTCAAACCGCTGTGCGTT+TGG	0.390239	1.3:+47861745	MS.gene073851:CDS
TTTGGTTCTTCCTGGTGAAT+TGG	0.398260	1.3:+47861763	MS.gene073851:CDS
TTTCTCTGCCTTGGGAGCCA+TGG	0.402280	1.3:-47861429	None:intergenic
AAACAGGTTCATCCTGATAT+AGG	0.421612	1.3:+47861602	MS.gene073851:CDS
AGGTATTTCTTCAAAAGCTA+TGG	0.429032	1.3:+47861622	MS.gene073851:CDS
CTTGTTGTAACGAGCCAATC+TGG	0.445538	1.3:-47861699	None:intergenic
TCCGCGGGTTTCTTCTCAGC+GGG	0.464857	1.3:-47861455	None:intergenic
TCTGCTGGTGATTTCTCCGC+GGG	0.470002	1.3:-47861470	None:intergenic
CGCTCAGGAAGCTTCCAGAT+TGG	0.475450	1.3:+47861685	MS.gene073851:CDS
TGCTGTTTCTGAAGGAACTA+AGG	0.493611	1.3:+47861793	MS.gene073851:CDS
GCATGTTTCGCCAATTCACC+AGG	0.516345	1.3:-47861773	None:intergenic
GGTATTTCTTCAAAAGCTAT+GGG	0.517184	1.3:+47861623	MS.gene073851:CDS
GCGAAACATGCTGTTTCTGA+AGG	0.530754	1.3:+47861785	MS.gene073851:CDS
ATTCAGATCCATGGCTCCCA+AGG	0.541897	1.3:+47861421	None:intergenic
GAAACCGACGATTACGTCAC+GGG	0.556515	1.3:+47861721	MS.gene073851:CDS
CTCTGCTGGTGATTTCTCCG+CGG	0.560516	1.3:-47861471	None:intergenic
GAGAAGAAGATCTCGAAAGA+AGG	0.564931	1.3:+47861509	MS.gene073851:CDS
TATCTTTGAGAAACTCGCTC+AGG	0.586744	1.3:+47861670	MS.gene073851:CDS
ATCTCCCGTGACGTAATCGT+CGG	0.590908	1.3:-47861725	None:intergenic
CTCCGCGGGTTTCTTCTCAG+CGG	0.628471	1.3:-47861456	None:intergenic
AAGAAGATCTCGAAAGAAGG+AGG	0.628797	1.3:+47861512	MS.gene073851:CDS
AGAAACCGACGATTACGTCA+CGG	0.672005	1.3:+47861720	MS.gene073851:CDS
ACCCGCTGAGAAGAAACCCG+CGG	0.690098	1.3:+47861454	MS.gene073851:CDS
AGGAAGAACCAAACGCACAG+CGG	0.757762	1.3:-47861753	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTATAAGATTTACATCTTCA+AGG	+	chr1.3:47861574-47861593	MS.gene073851:CDS	25.0%
!	CTTGAAGATGTAAATCTTAT+AGG	-	chr1.3:47861576-47861595	None:intergenic	25.0%
!	TTTGAAGAAATACCTATATC+AGG	-	chr1.3:47861617-47861636	None:intergenic	25.0%
	AGGTATTTCTTCAAAAGCTA+TGG	+	chr1.3:47861622-47861641	MS.gene073851:CDS	30.0%
	GGTATTTCTTCAAAAGCTAT+GGG	+	chr1.3:47861623-47861642	MS.gene073851:CDS	30.0%
	AAACAGGTTCATCCTGATAT+AGG	+	chr1.3:47861602-47861621	MS.gene073851:CDS	35.0%
	TTCTTTCGAGATCTTCTTCT+CGG	-	chr1.3:47861510-47861529	None:intergenic	35.0%
	AAGAAGATCTCGAAAGAAGG+AGG	+	chr1.3:47861512-47861531	MS.gene073851:CDS	40.0%
	CATCTTCAAGGTTCTCAAAC+AGG	+	chr1.3:47861586-47861605	MS.gene073851:CDS	40.0%
	GAGAAGAAGATCTCGAAAGA+AGG	+	chr1.3:47861509-47861528	MS.gene073851:CDS	40.0%
!	TATCTTTGAGAAACTCGCTC+AGG	+	chr1.3:47861670-47861689	MS.gene073851:CDS	40.0%
!!	TGCTGTTTCTGAAGGAACTA+AGG	+	chr1.3:47861793-47861812	MS.gene073851:CDS	40.0%
!!	TTTGGTTCTTCCTGGTGAAT+TGG	+	chr1.3:47861763-47861782	MS.gene073851:CDS	40.0%
	AGAAACCGACGATTACGTCA+CGG	+	chr1.3:47861720-47861739	MS.gene073851:CDS	45.0%
!	CTTGTTGTAACGAGCCAATC+TGG	-	chr1.3:47861702-47861721	None:intergenic	45.0%
!!	GCGAAACATGCTGTTTCTGA+AGG	+	chr1.3:47861785-47861804	MS.gene073851:CDS	45.0%
	AGGAAGAACCAAACGCACAG+CGG	-	chr1.3:47861756-47861775	None:intergenic	50.0%
	ATCTCCCGTGACGTAATCGT+CGG	-	chr1.3:47861728-47861747	None:intergenic	50.0%
	GAAACCGACGATTACGTCAC+GGG	+	chr1.3:47861721-47861740	MS.gene073851:CDS	50.0%
	GAGATCTTCTTCTCGGCTCT+TGG	-	chr1.3:47861503-47861522	None:intergenic	50.0%
	GATTCAAACCGCTGTGCGTT+TGG	+	chr1.3:47861745-47861764	MS.gene073851:CDS	50.0%
	GCATGTTTCGCCAATTCACC+AGG	-	chr1.3:47861776-47861795	None:intergenic	50.0%
!!	GCTCTTGGCTTTTTCTCTGC+TGG	-	chr1.3:47861488-47861507	None:intergenic	50.0%
!!!	AGCGGGTTTTTTCTCTGCCT+TGG	-	chr1.3:47861441-47861460	None:intergenic	50.0%
!!!	GCGGGTTTTTTCTCTGCCTT+GGG	-	chr1.3:47861440-47861459	None:intergenic	50.0%
	CGCTCAGGAAGCTTCCAGAT+TGG	+	chr1.3:47861685-47861704	MS.gene073851:CDS	55.0%
	CTCTGCTGGTGATTTCTCCG+CGG	-	chr1.3:47861474-47861493	None:intergenic	55.0%
	TCTGCTGGTGATTTCTCCGC+GGG	-	chr1.3:47861473-47861492	None:intergenic	55.0%
!!	GCTGTGCGTTTGGTTCTTCC+TGG	+	chr1.3:47861755-47861774	MS.gene073851:CDS	55.0%
	ACCCGCTGAGAAGAAACCCG+CGG	+	chr1.3:47861454-47861473	MS.gene073851:CDS	60.0%
	CTCCGCGGGTTTCTTCTCAG+CGG	-	chr1.3:47861459-47861478	None:intergenic	60.0%
	TCCGCGGGTTTCTTCTCAGC+GGG	-	chr1.3:47861458-47861477	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	47861431	47861817	47861431	ID=MS.gene073851
chr1.3	mRNA	47861431	47861817	47861431	ID=MS.gene073851.t1;Parent=MS.gene073851
chr1.3	exon	47861431	47861817	47861431	ID=MS.gene073851.t1.exon1;Parent=MS.gene073851.t1
chr1.3	CDS	47861431	47861817	47861431	ID=cds.MS.gene073851.t1;Parent=MS.gene073851.t1

Gene Sequence

>MS.gene073851

ATGGCTCCCAAGGCAGAGAAAAAACCCGCTGAGAAGAAACCCGCGGAGAAATCACCAGCAGAGAAAAAGCCAAGAGCCGAGAAGAAGATCTCGAAAGAAGGAGGCGATAAGAAGAAGAAGCGCGTGAAGAAGAGCGTTGAAACCTATAAGATTTACATCTTCAAGGTTCTCAAACAGGTTCATCCTGATATAGGTATTTCTTCAAAAGCTATGGGAATTATGAACAGTTTCATCAATGATATCTTTGAGAAACTCGCTCAGGAAGCTTCCAGATTGGCTCGTTACAACAAGAAACCGACGATTACGTCACGGGAGATTCAAACCGCTGTGCGTTTGGTTCTTCCTGGTGAATTGGCGAAACATGCTGTTTCTGAAGGAACTAAGGCT

Protein sequence

>MS.gene073851.t1

MAPKAEKKPAEKKPAEKSPAEKKPRAEKKISKEGGDKKKKRVKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQEASRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKA