AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene074358


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074358.t1 MTR_3g077890 94.198 293 9 2 1 293 1 285 6.46e-177 489
MS.gene074358.t1 MTR_4g105750 46.930 228 70 11 42 267 23 201 3.59e-44 149
MS.gene074358.t1 MTR_5g034230 35.111 225 89 10 99 293 51 248 3.70e-21 90.5
MS.gene074358.t1 MTR_8g064650 29.885 261 136 10 62 293 78 320 2.99e-18 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074358.t1 AT5G62770 33.564 289 131 12 33 293 13 268 1.18e-26 105
MS.gene074358.t1 AT1G70420 30.164 305 131 13 31 293 8 272 1.66e-21 92.0
MS.gene074358.t1 AT3G27880 25.938 320 122 10 11 293 1 242 1.99e-14 71.6
MS.gene074358.t1 AT3G01513 29.050 179 94 5 134 288 47 216 3.93e-11 62.0
MS.gene074358.t1 AT1G23710 31.925 213 109 11 33 235 8 194 5.86e-11 62.4

Find 69 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTCAATTCCAACCGAATT+AGG 0.102682 3.4:-74746857 None:intergenic
GAGAAAAGTCCAACCAATTC+AGG 0.278046 3.4:-74747079 None:intergenic
CGTCGTGTTGTTGTTTCTTC+AGG 0.283860 3.4:-74746710 None:intergenic
CGACGAGTAACTGCTGAAAC+TGG 0.310454 3.4:-74746360 None:intergenic
TTCTCTAATGTTAATGGGCT+TGG 0.310521 3.4:+74747097 MS.gene074358:CDS
GCTCAATTCCAACCGAATTA+GGG 0.337397 3.4:-74746856 None:intergenic
AAGAGTAGTTCCACAGGTTC+AGG 0.339929 3.4:+74746884 MS.gene074358:CDS
TCCCTTCCGCTACACCTTCC+AGG 0.340690 3.4:-74746817 None:intergenic
TCGAAGACTGGATAATATAA+AGG 0.352379 3.4:-74746632 None:intergenic
TCGAACATCAGCGTTCTTAA+CGG 0.355291 3.4:-74746740 None:intergenic
GTTGTTGTTTCTTCAGGAAC+CGG 0.406609 3.4:-74746704 None:intergenic
CCAACCAATTCAGGCTTGTA+AGG 0.415111 3.4:-74747070 None:intergenic
CTGCGTGTGGAACCCTAATT+CGG 0.421684 3.4:+74746844 MS.gene074358:CDS
TTTGCCTTACAAGCCTGAAT+TGG 0.426620 3.4:+74747066 MS.gene074358:CDS
AACCGATGAGAGTGTGGACC+TGG 0.461373 3.4:+74746799 MS.gene074358:CDS
GGATAGTGAATTTGAGTTTG+CGG 0.468810 3.4:+74746538 MS.gene074358:CDS
TTGATAAAGCGCGTCTTCGT+CGG 0.475206 3.4:-74746441 None:intergenic
AGCGCGTCTTCGTCGGAATG+AGG 0.479185 3.4:-74746434 None:intergenic
GTGGAAAGAATCAGAGTAAG+AGG 0.485953 3.4:+74747035 MS.gene074358:CDS
TGAGTTTGCGGTGGTTTCGA+CGG 0.490223 3.4:+74746550 MS.gene074358:CDS
TTCTTCAGGAACCGGCGAAA+CGG 0.490817 3.4:-74746696 None:intergenic
GAGTAAAGTGTCGCCGGCGG+TGG 0.493944 3.4:+74747003 MS.gene074358:CDS
TGTCGTTTCTTCCGTTTCGC+CGG 0.501674 3.4:+74746685 MS.gene074358:CDS
AAGACGCGCTTTATCAACCT+TGG 0.509179 3.4:+74746447 MS.gene074358:CDS
CGATGGGAAGGATAAACAAC+CGG 0.517185 3.4:+74746952 MS.gene074358:CDS
CCTTACAAGCCTGAATTGGT+TGG 0.518307 3.4:+74747070 MS.gene074358:CDS
CGAAGAAAGCAGCGAAGCTA+CGG 0.524503 3.4:+74746760 MS.gene074358:CDS
CCGCAACTACAGAGAGATCA+AGG 0.532253 3.4:+74746284 MS.gene074358:CDS
GATCGAAGCGGTGGAAGCTA+CGG 0.532508 3.4:+74746906 MS.gene074358:CDS
CCACCGTCATTCTCCACCGC+CGG 0.535765 3.4:-74747016 None:intergenic
TTCCAGGTCCACACTCTCAT+CGG 0.542516 3.4:-74746801 None:intergenic
CCTTGATCTCTCTGTAGTTG+CGG 0.545049 3.4:-74746284 None:intergenic
AGACGCGCTTTATCAACCTT+GGG 0.545606 3.4:+74746448 MS.gene074358:CDS
AGCATAAGCTATCATGATTG+AGG 0.548556 3.4:+74746247 None:intergenic
CTATTAAACGACGACGATGA+CGG 0.549652 3.4:+74746662 MS.gene074358:CDS
TTCGGAATCTGAAACATCAC+CGG 0.550899 3.4:-74746971 None:intergenic
GCGCGTCTTCGTCGGAATGA+GGG 0.555203 3.4:-74746433 None:intergenic
CTAATGTTAATGGGCTTGGT+CGG 0.556128 3.4:+74747101 MS.gene074358:CDS
TCGCCGGCGGTGGAGAATGA+CGG 0.571185 3.4:+74747013 MS.gene074358:CDS
CGTCGGAATGAGGGTCCCAC+CGG 0.576002 3.4:-74746424 None:intergenic
CTAGAGTCATCGATGAGCTC+CGG 0.578914 3.4:+74746405 MS.gene074358:CDS
CCGCTTCGATCCTGAACCTG+TGG 0.579975 3.4:-74746894 None:intergenic
GACCTGGAAGGTGTAGCGGA+AGG 0.580315 3.4:+74746815 MS.gene074358:CDS
GAGTCATCGATGAGCTCCGG+TGG 0.581333 3.4:+74746408 MS.gene074358:CDS
ATTGTGATTATGAGAAGAAG+CGG 0.581372 3.4:-74746321 None:intergenic
CGGAAGGGAGTTACTGCGTG+TGG 0.582157 3.4:+74746831 MS.gene074358:CDS
GAGGAGTCATAGCGATGGGA+AGG 0.587806 3.4:+74746940 MS.gene074358:CDS
CAGGTTCAGGATCGAAGCGG+TGG 0.589038 3.4:+74746897 MS.gene074358:CDS
GACGAGTAACTGCTGAAACT+GGG 0.589040 3.4:-74746359 None:intergenic
ACCTGGAAGGTGTAGCGGAA+GGG 0.590239 3.4:+74746816 MS.gene074358:CDS
GACGGCGAGTAAAGTGTCGC+CGG 0.603567 3.4:+74746997 MS.gene074358:CDS
CAGTTTCAGCAGTTACTCGT+CGG 0.608931 3.4:+74746361 MS.gene074358:CDS
ATCTTCAACCGATGAGAGTG+TGG 0.611370 3.4:+74746793 MS.gene074358:CDS
TAACTGCTGAAACTGGGAGA+AGG 0.615139 3.4:-74746353 None:intergenic
TGTGGACCTGGAAGGTGTAG+CGG 0.615287 3.4:+74746811 MS.gene074358:CDS
AAACGACAACGAAGAAGACG+AGG 0.630831 3.4:+74746517 MS.gene074358:CDS
ACCGTTGTAGAAAATGTCAT+CGG 0.634438 3.4:-74746600 None:intergenic
TAGTGAATTTGAGTTTGCGG+TGG 0.640807 3.4:+74746541 MS.gene074358:CDS
GGCGAGTAAAGTGTCGCCGG+CGG 0.643569 3.4:+74747000 MS.gene074358:CDS
GTGTGGAACCCTAATTCGGT+TGG 0.656223 3.4:+74746848 MS.gene074358:CDS
CCGGCGGTGGAGAATGACGG+TGG 0.663067 3.4:+74747016 MS.gene074358:CDS
ATAAGCTATCATGATTGAGG+TGG 0.665763 3.4:+74746250 None:intergenic
GGATTCAACAAATTTCTCAG+TGG 0.667196 3.4:+74746571 MS.gene074358:CDS
AGTCATCGATGAGCTCCGGT+GGG 0.674608 3.4:+74746409 MS.gene074358:CDS
GTTTCAGATTCCGAAAACGA+CGG 0.693270 3.4:+74746979 MS.gene074358:CDS
CCACAGGTTCAGGATCGAAG+CGG 0.701914 3.4:+74746894 MS.gene074358:CDS
GTTGTAGAAAATGTCATCGG+CGG 0.703917 3.4:-74746597 None:intergenic
GATGAGAGTGTGGACCTGGA+AGG 0.711100 3.4:+74746803 MS.gene074358:CDS
GAGCTCATCGATGACTCTAG+CGG 0.730084 3.4:-74746402 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GACTTTTCTCTAATGTTAAT+GGG + chr3.4:74747092-74747111 MS.gene074358:CDS 25.0%
ATTGTGATTATGAGAAGAAG+CGG - chr3.4:74746324-74746343 None:intergenic 30.0%
TCGAAGACTGGATAATATAA+AGG - chr3.4:74746635-74746654 None:intergenic 30.0%
! GGACTTTTCTCTAATGTTAA+TGG + chr3.4:74747091-74747110 MS.gene074358:CDS 30.0%
!! AATAGATTTTGATCGAAGAC+TGG - chr3.4:74746647-74746666 None:intergenic 30.0%
!!! AATTTGTTGTCATTTTCCCA+AGG - chr3.4:74746467-74746486 None:intergenic 30.0%
ACCGTTGTAGAAAATGTCAT+CGG - chr3.4:74746603-74746622 None:intergenic 35.0%
GGATTCAACAAATTTCTCAG+TGG + chr3.4:74746571-74746590 MS.gene074358:CDS 35.0%
! GGATAGTGAATTTGAGTTTG+CGG + chr3.4:74746538-74746557 MS.gene074358:CDS 35.0%
!! TTCTCTAATGTTAATGGGCT+TGG + chr3.4:74747097-74747116 MS.gene074358:CDS 35.0%
CGAAAAAAGAGTAGTTCCAC+AGG + chr3.4:74746878-74746897 MS.gene074358:CDS 40.0%
CTATTAAACGACGACGATGA+CGG + chr3.4:74746662-74746681 MS.gene074358:CDS 40.0%
GAGAAAAGTCCAACCAATTC+AGG - chr3.4:74747082-74747101 None:intergenic 40.0%
GCTCAATTCCAACCGAATTA+GGG - chr3.4:74746859-74746878 None:intergenic 40.0%
GTGGAAAGAATCAGAGTAAG+AGG + chr3.4:74747035-74747054 MS.gene074358:CDS 40.0%
GTTGTAGAAAATGTCATCGG+CGG - chr3.4:74746600-74746619 None:intergenic 40.0%
GTTTCAGATTCCGAAAACGA+CGG + chr3.4:74746979-74746998 MS.gene074358:CDS 40.0%
TCGAACATCAGCGTTCTTAA+CGG - chr3.4:74746743-74746762 None:intergenic 40.0%
TTTGCCTTACAAGCCTGAAT+TGG + chr3.4:74747066-74747085 MS.gene074358:CDS 40.0%
! GCCGATGACATTTTCTACAA+CGG + chr3.4:74746599-74746618 MS.gene074358:CDS 40.0%
! TAGTGAATTTGAGTTTGCGG+TGG + chr3.4:74746541-74746560 MS.gene074358:CDS 40.0%
! TTCGGAATCTGAAACATCAC+CGG - chr3.4:74746974-74746993 None:intergenic 40.0%
!! CTAATGTTAATGGGCTTGGT+CGG + chr3.4:74747101-74747120 MS.gene074358:CDS 40.0%
!! GTTGTTGTTTCTTCAGGAAC+CGG - chr3.4:74746707-74746726 None:intergenic 40.0%
!! TTTTGAGGAGTCATAGCGAT+GGG + chr3.4:74746936-74746955 MS.gene074358:CDS 40.0%
!!! TTCGTTGTCGTTTTGCTTAG+CGG - chr3.4:74746510-74746529 None:intergenic 40.0%
AAACGACAACGAAGAAGACG+AGG + chr3.4:74746517-74746536 MS.gene074358:CDS 45.0%
AAGACGCGCTTTATCAACCT+TGG + chr3.4:74746447-74746466 MS.gene074358:CDS 45.0%
AAGAGTAGTTCCACAGGTTC+AGG + chr3.4:74746884-74746903 MS.gene074358:CDS 45.0%
AGACGCGCTTTATCAACCTT+GGG + chr3.4:74746448-74746467 MS.gene074358:CDS 45.0%
ATCTTCAACCGATGAGAGTG+TGG + chr3.4:74746793-74746812 MS.gene074358:CDS 45.0%
CAGTTTCAGCAGTTACTCGT+CGG + chr3.4:74746361-74746380 MS.gene074358:CDS 45.0%
CCAACCAATTCAGGCTTGTA+AGG - chr3.4:74747073-74747092 None:intergenic 45.0%
CCTTACAAGCCTGAATTGGT+TGG + chr3.4:74747070-74747089 MS.gene074358:CDS 45.0%
CCTTGATCTCTCTGTAGTTG+CGG - chr3.4:74746287-74746306 None:intergenic 45.0%
CGATGGGAAGGATAAACAAC+CGG + chr3.4:74746952-74746971 MS.gene074358:CDS 45.0%
CGCTCAATTCCAACCGAATT+AGG - chr3.4:74746860-74746879 None:intergenic 45.0%
GACGAGTAACTGCTGAAACT+GGG - chr3.4:74746362-74746381 None:intergenic 45.0%
TAACTGCTGAAACTGGGAGA+AGG - chr3.4:74746356-74746375 None:intergenic 45.0%
TTGATAAAGCGCGTCTTCGT+CGG - chr3.4:74746444-74746463 None:intergenic 45.0%
!! ACTTTACTCGCCGTCGTTTT+CGG - chr3.4:74746992-74747011 None:intergenic 45.0%
!! AGCTACGGAACTTGCTTTTG+AGG + chr3.4:74746921-74746940 MS.gene074358:CDS 45.0%
!! CGTCGTGTTGTTGTTTCTTC+AGG - chr3.4:74746713-74746732 None:intergenic 45.0%
!! CTTTTGAGGAGTCATAGCGA+TGG + chr3.4:74746935-74746954 MS.gene074358:CDS 45.0%
CCGCAACTACAGAGAGATCA+AGG + chr3.4:74746284-74746303 MS.gene074358:CDS 50.0%
CGAAGAAAGCAGCGAAGCTA+CGG + chr3.4:74746760-74746779 MS.gene074358:CDS 50.0%
CGACGAGTAACTGCTGAAAC+TGG - chr3.4:74746363-74746382 None:intergenic 50.0%
CTAGAGTCATCGATGAGCTC+CGG + chr3.4:74746405-74746424 MS.gene074358:CDS 50.0%
CTGCGTGTGGAACCCTAATT+CGG + chr3.4:74746844-74746863 MS.gene074358:CDS 50.0%
GAGCTCATCGATGACTCTAG+CGG - chr3.4:74746405-74746424 None:intergenic 50.0%
GTGTGGAACCCTAATTCGGT+TGG + chr3.4:74746848-74746867 MS.gene074358:CDS 50.0%
TGAGTTTGCGGTGGTTTCGA+CGG + chr3.4:74746550-74746569 MS.gene074358:CDS 50.0%
TTCCAGGTCCACACTCTCAT+CGG - chr3.4:74746804-74746823 None:intergenic 50.0%
TTCTTCAGGAACCGGCGAAA+CGG - chr3.4:74746699-74746718 None:intergenic 50.0%
! TGTCGTTTCTTCCGTTTCGC+CGG + chr3.4:74746685-74746704 MS.gene074358:CDS 50.0%
AGTCATCGATGAGCTCCGGT+GGG + chr3.4:74746409-74746428 MS.gene074358:CDS 55.0%
CCACAGGTTCAGGATCGAAG+CGG + chr3.4:74746894-74746913 MS.gene074358:CDS 55.0%
GAGGAGTCATAGCGATGGGA+AGG + chr3.4:74746940-74746959 MS.gene074358:CDS 55.0%
GATCGAAGCGGTGGAAGCTA+CGG + chr3.4:74746906-74746925 MS.gene074358:CDS 55.0%
! AACCGATGAGAGTGTGGACC+TGG + chr3.4:74746799-74746818 MS.gene074358:CDS 55.0%
! ACCTGGAAGGTGTAGCGGAA+GGG + chr3.4:74746816-74746835 MS.gene074358:CDS 55.0%
! GATGAGAGTGTGGACCTGGA+AGG + chr3.4:74746803-74746822 MS.gene074358:CDS 55.0%
! TGTGGACCTGGAAGGTGTAG+CGG + chr3.4:74746811-74746830 MS.gene074358:CDS 55.0%
AGCGCGTCTTCGTCGGAATG+AGG - chr3.4:74746437-74746456 None:intergenic 60.0%
CAGGTTCAGGATCGAAGCGG+TGG + chr3.4:74746897-74746916 MS.gene074358:CDS 60.0%
CCGCTTCGATCCTGAACCTG+TGG - chr3.4:74746897-74746916 None:intergenic 60.0%
CGGAAGGGAGTTACTGCGTG+TGG + chr3.4:74746831-74746850 MS.gene074358:CDS 60.0%
GAGTCATCGATGAGCTCCGG+TGG + chr3.4:74746408-74746427 MS.gene074358:CDS 60.0%
GCGCGTCTTCGTCGGAATGA+GGG - chr3.4:74746436-74746455 None:intergenic 60.0%
TCCCTTCCGCTACACCTTCC+AGG - chr3.4:74746820-74746839 None:intergenic 60.0%
! GACCTGGAAGGTGTAGCGGA+AGG + chr3.4:74746815-74746834 MS.gene074358:CDS 60.0%
!! GACGGCGAGTAAAGTGTCGC+CGG + chr3.4:74746997-74747016 MS.gene074358:CDS 60.0%
CCACCGTCATTCTCCACCGC+CGG - chr3.4:74747019-74747038 None:intergenic 65.0%
CGTCGGAATGAGGGTCCCAC+CGG - chr3.4:74746427-74746446 None:intergenic 65.0%
TCGCCGGCGGTGGAGAATGA+CGG + chr3.4:74747013-74747032 MS.gene074358:CDS 65.0%
!! GAGTAAAGTGTCGCCGGCGG+TGG + chr3.4:74747003-74747022 MS.gene074358:CDS 65.0%
!! GGCGAGTAAAGTGTCGCCGG+CGG + chr3.4:74747000-74747019 MS.gene074358:CDS 65.0%
CCGGCGGTGGAGAATGACGG+TGG + chr3.4:74747016-74747035 MS.gene074358:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 74746260 74747141 74746260 ID=MS.gene074358
chr3.4 mRNA 74746260 74747141 74746260 ID=MS.gene074358.t1;Parent=MS.gene074358
chr3.4 exon 74746260 74747141 74746260 ID=MS.gene074358.t1.exon1;Parent=MS.gene074358.t1
chr3.4 CDS 74746260 74747141 74746260 ID=cds.MS.gene074358.t1;Parent=MS.gene074358.t1
Gene Sequence

>MS.gene074358

ATGATTGAGGTGGAAACGATGCAGCCGCAACTACAGAGAGATCAAGGAGAAGCTTCTTTTTCCGCTTCTTCTCATAATCACAATCACGTTTCTCCTTCTCCCAGTTTCAGCAGTTACTCGTCGGAAACACTCGCTGAAATCGCCGCTAGAGTCATCGATGAGCTCCGGTGGGACCCTCATTCCGACGAAGACGCGCTTTATCAACCTTGGGAAAATGACAACAAATTAACGACTCAAAACGACGACTCCGCTAAGCAAAACGACAACGAAGAAGACGAGGATAGTGAATTTGAGTTTGCGGTGGTTTCGACGGATTCAACAAATTTCTCAGTGGTTTCCGCCGATGACATTTTCTACAACGGTCAGATCAAACCTTTATATTATCCAGTCTTCGATCAAAATCTATTAAACGACGACGATGACGGTGTCGTTTCTTCCGTTTCGCCGGTTCCTGAAGAAACAACAACACGACGTCGTTTACCGTTAAGAACGCTGATGTTCGAAGAAAGCAGCGAAGCTACGGCGTCGTGTTCATCTTCAACCGATGAGAGTGTGGACCTGGAAGGTGTAGCGGAAGGGAGTTACTGCGTGTGGAACCCTAATTCGGTTGGAATTGAGCGAAAAAAGAGTAGTTCCACAGGTTCAGGATCGAAGCGGTGGAAGCTACGGAACTTGCTTTTGAGGAGTCATAGCGATGGGAAGGATAAACAACCGGTGATGTTTCAGATTCCGAAAACGACGGCGAGTAAAGTGTCGCCGGCGGTGGAGAATGACGGTGGAAAGAATCAGAGTAAGAGGAAGTCGTTTTTGCCTTACAAGCCTGAATTGGTTGGACTTTTCTCTAATGTTAATGGGCTTGGTCGGAATTTGAACCCGTTTTGA

Protein sequence

>MS.gene074358.t1

MIEVETMQPQLQRDQGEASFSASSHNHNHVSPSPSFSSYSSETLAEIAARVIDELRWDPHSDEDALYQPWENDNKLTTQNDDSAKQNDNEEDEDSEFEFAVVSTDSTNFSVVSADDIFYNGQIKPLYYPVFDQNLLNDDDDGVVSSVSPVPEETTTRRRLPLRTLMFEESSEATASCSSSTDESVDLEGVAEGSYCVWNPNSVGIERKKSSSTGSGSKRWKLRNLLLRSHSDGKDKQPVMFQIPKTTASKVSPAVENDGGKNQSKRKSFLPYKPELVGLFSNVNGLGRNLNPF