Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08288.t1 | AES99193.1 | 80.4 | 92 | 15 | 1 | 1 | 92 | 1 | 89 | 6.50E-27 | 129.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08288.t1 | G7KG06 | 80.4 | 92 | 15 | 1 | 1 | 92 | 1 | 89 | 4.7e-27 | 129.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050133 | MS.gene08288 | -0.806349 | 8.59E-50 | -1.69E-46 |
| MS.gene05081 | MS.gene08288 | 0.867673 | 1.15E-65 | -1.69E-46 |
| MS.gene051455 | MS.gene08288 | 0.801603 | 8.33E-49 | -1.69E-46 |
| MS.gene051838 | MS.gene08288 | 0.863539 | 2.32E-64 | -1.69E-46 |
| MS.gene052134 | MS.gene08288 | 0.807291 | 5.43E-50 | -1.69E-46 |
| MS.gene052469 | MS.gene08288 | 0.852039 | 5.99E-61 | -1.69E-46 |
| MS.gene053824 | MS.gene08288 | 0.801702 | 7.95E-49 | -1.69E-46 |
| MS.gene054107 | MS.gene08288 | 0.806086 | 9.76E-50 | -1.69E-46 |
| MS.gene054810 | MS.gene08288 | -0.801334 | 9.46E-49 | -1.69E-46 |
| MS.gene054884 | MS.gene08288 | -0.816689 | 4.86E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene08288 | 0.81323 | 2.84E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene08288 | 0.810762 | 9.81E-51 | -1.69E-46 |
| MS.gene055539 | MS.gene08288 | 0.807369 | 5.23E-50 | -1.69E-46 |
| MS.gene055741 | MS.gene08288 | 0.807931 | 3.97E-50 | -1.69E-46 |
| MS.gene055744 | MS.gene08288 | 0.808412 | 3.14E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene08288 | 0.802737 | 4.87E-49 | -1.69E-46 |
| MS.gene057332 | MS.gene08288 | 0.827587 | 1.44E-54 | -1.69E-46 |
| MS.gene058838 | MS.gene08288 | 0.818183 | 2.24E-52 | -1.69E-46 |
| MS.gene059717 | MS.gene08288 | 0.805231 | 1.47E-49 | -1.69E-46 |
| MS.gene060835 | MS.gene08288 | 0.815204 | 1.04E-51 | -1.69E-46 |
| MS.gene061154 | MS.gene08288 | 0.809697 | 1.66E-50 | -1.69E-46 |
| MS.gene061231 | MS.gene08288 | 0.823762 | 1.16E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08288.t1 | MTR_4g083110 | 79.121 | 91 | 12 | 1 | 1 | 91 | 1 | 84 | 3.00e-39 | 125 |
| MS.gene08288.t1 | MTR_5g077640 | 80.435 | 92 | 15 | 1 | 1 | 92 | 1 | 89 | 7.26e-39 | 124 |
| MS.gene08288.t1 | MTR_4g083130 | 79.348 | 92 | 16 | 1 | 1 | 92 | 1 | 89 | 2.89e-33 | 110 |
| MS.gene08288.t1 | MTR_5g077680 | 96.154 | 52 | 2 | 0 | 1 | 52 | 1 | 52 | 3.29e-32 | 107 |
| MS.gene08288.t1 | MTR_8g079458 | 96.154 | 52 | 2 | 0 | 1 | 52 | 1 | 52 | 3.29e-32 | 107 |
| MS.gene08288.t1 | MTR_4g012020 | 94.231 | 52 | 3 | 0 | 1 | 52 | 1 | 52 | 1.24e-31 | 105 |
| MS.gene08288.t1 | MTR_1437s0010 | 92.308 | 52 | 4 | 0 | 1 | 52 | 1 | 52 | 3.88e-31 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08288.t1 | AT1G30260 | 59.574 | 47 | 18 | 1 | 1 | 47 | 1 | 46 | 4.36e-15 | 64.7 |
Find 12 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATTGCTGAAGAGGATA+GGG | 0.483802 | 4.2:-15368804 | MS.gene08288:CDS |
| AAACAATTGCTGAAGAGGAT+AGG | 0.497259 | 4.2:-15368805 | MS.gene08288:CDS |
| GATGGAAACAATTGCTGAAG+AGG | 0.505582 | 4.2:-15368810 | MS.gene08288:CDS |
| GCATAGAACATCAAAGATGA+TGG | 0.512561 | 4.2:-15368828 | MS.gene08288:CDS |
| AACAGTTGCACTTGTAGAAG+AGG | 0.528743 | 4.2:+15368697 | None:intergenic |
| CACAATATGTTTCTGTGATG+AGG | 0.546938 | 4.2:-15368850 | MS.gene08288:CDS |
| GACGGAGCAAGTGATGAAGC+TGG | 0.554923 | 4.2:+15368728 | None:intergenic |
| TGGCAAGTTCTCGCATTGCA+AGG | 0.566271 | 4.2:-15368895 | MS.gene08288:CDS |
| GTAGAAGAGGCGCATGCAGA+CGG | 0.588748 | 4.2:+15368710 | None:intergenic |
| CTCATCACAGAAACATATTG+TGG | 0.621625 | 4.2:+15368851 | None:intergenic |
| TAATACTAGATACTTTCTGA+AGG | 0.630145 | 4.2:-15368675 | MS.gene08288:CDS |
| ATCACAGAAACATATTGTGG+TGG | 0.672845 | 4.2:+15368854 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAATACTAGATACTTTCTGA+AGG | - | chr4.2:15368861-15368880 | MS.gene08288:CDS | 25.0% |
| !! | GGAATTGTTGATGAACTTTT+AGG | + | chr4.2:15368790-15368809 | None:intergenic | 30.0% |
| AAACAATTGCTGAAGAGGAT+AGG | - | chr4.2:15368731-15368750 | MS.gene08288:CDS | 35.0% | |
| AACAATTGCTGAAGAGGATA+GGG | - | chr4.2:15368732-15368751 | MS.gene08288:CDS | 35.0% | |
| ATCACAGAAACATATTGTGG+TGG | + | chr4.2:15368685-15368704 | None:intergenic | 35.0% | |
| CTCATCACAGAAACATATTG+TGG | + | chr4.2:15368688-15368707 | None:intergenic | 35.0% | |
| GCATAGAACATCAAAGATGA+TGG | - | chr4.2:15368708-15368727 | MS.gene08288:CDS | 35.0% | |
| ! | CACAATATGTTTCTGTGATG+AGG | - | chr4.2:15368686-15368705 | MS.gene08288:CDS | 35.0% |
| AACAGTTGCACTTGTAGAAG+AGG | + | chr4.2:15368842-15368861 | None:intergenic | 40.0% | |
| GATGGAAACAATTGCTGAAG+AGG | - | chr4.2:15368726-15368745 | MS.gene08288:CDS | 40.0% | |
| ! | CATTGCAAGGTTTTTCATGG+AGG | - | chr4.2:15368654-15368673 | MS.gene08288:CDS | 40.0% |
| ! | TCGCATTGCAAGGTTTTTCA+TGG | - | chr4.2:15368651-15368670 | MS.gene08288:CDS | 40.0% |
| TGGCAAGTTCTCGCATTGCA+AGG | - | chr4.2:15368641-15368660 | MS.gene08288:CDS | 50.0% | |
| GACGGAGCAAGTGATGAAGC+TGG | + | chr4.2:15368811-15368830 | None:intergenic | 55.0% | |
| ! | GTAGAAGAGGCGCATGCAGA+CGG | + | chr4.2:15368829-15368848 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 15368640 | 15368918 | 15368640 | ID=MS.gene08288 |
| chr4.2 | mRNA | 15368640 | 15368918 | 15368640 | ID=MS.gene08288.t1;Parent=MS.gene08288 |
| chr4.2 | exon | 15368640 | 15368918 | 15368640 | ID=MS.gene08288.t1.exon1;Parent=MS.gene08288.t1 |
| chr4.2 | CDS | 15368640 | 15368918 | 15368640 | ID=cds.MS.gene08288.t1;Parent=MS.gene08288.t1 |
| Gene Sequence |
| Protein sequence |
