Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09868.t1 | XP_003625486.1 | 100 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.70E-21 | 111.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09868.t1 | Q2HT99 | 100.0 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.0e-21 | 111.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene09868 | 0.86417 | 1.47E-64 | -1.69E-46 |
| MS.gene050563 | MS.gene09868 | 0.810204 | 1.29E-50 | -1.69E-46 |
| MS.gene050584 | MS.gene09868 | 0.902138 | 1.32E-78 | -1.69E-46 |
| MS.gene050585 | MS.gene09868 | 0.896862 | 2.45E-76 | -1.69E-46 |
| MS.gene050586 | MS.gene09868 | 0.822367 | 2.46E-53 | -1.69E-46 |
| MS.gene050587 | MS.gene09868 | 0.835072 | 2.08E-56 | -1.69E-46 |
| MS.gene050807 | MS.gene09868 | 0.842371 | 2.69E-58 | -1.69E-46 |
| MS.gene050808 | MS.gene09868 | 0.814234 | 1.71E-51 | -1.69E-46 |
| MS.gene05143 | MS.gene09868 | 0.806803 | 6.89E-50 | -1.69E-46 |
| MS.gene05157 | MS.gene09868 | 0.810165 | 1.32E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene09868 | 0.88978 | 1.78E-73 | -1.69E-46 |
| MS.gene052142 | MS.gene09868 | 0.848625 | 5.44E-60 | -1.69E-46 |
| MS.gene053074 | MS.gene09868 | 0.86032 | 2.24E-63 | -1.69E-46 |
| MS.gene053383 | MS.gene09868 | 0.843257 | 1.57E-58 | -1.69E-46 |
| MS.gene053563 | MS.gene09868 | 0.822123 | 2.80E-53 | -1.69E-46 |
| MS.gene05456 | MS.gene09868 | 0.856891 | 2.37E-62 | -1.69E-46 |
| MS.gene054596 | MS.gene09868 | 0.816078 | 6.66E-52 | -1.69E-46 |
| MS.gene055359 | MS.gene09868 | 0.834953 | 2.22E-56 | -1.69E-46 |
| MS.gene058168 | MS.gene09868 | 0.832747 | 7.92E-56 | -1.69E-46 |
| MS.gene059006 | MS.gene09868 | 0.901556 | 2.38E-78 | -1.69E-46 |
| MS.gene060691 | MS.gene09868 | 0.800729 | 1.26E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09868.t1 | MTR_7g099670 | 98.077 | 104 | 2 | 0 | 1 | 104 | 1 | 104 | 3.16e-60 | 179 |
| MS.gene09868.t1 | MTR_1g069085 | 68.000 | 50 | 14 | 2 | 1 | 49 | 1 | 49 | 5.67e-14 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGGTGGACTTGGTGGTTT+GGG | 0.222354 | 7.3:+12862549 | MS.gene09868:CDS |
| ACATTTGCTGGTGGTTATTC+AGG | 0.231052 | 7.3:+12862453 | MS.gene09868:CDS |
| CTTAGGTGGACTTGGTGGTT+TGG | 0.237390 | 7.3:+12862548 | MS.gene09868:CDS |
| CTTGGTGGTTTGGGAGGACT+TGG | 0.272357 | 7.3:+12862558 | MS.gene09868:CDS |
| TTAGGTGGTGGTGGTGGCTT+AGG | 0.291571 | 7.3:+12862531 | MS.gene09868:CDS |
| GGTGGAGCTGGTGGTGGTGT+TGG | 0.293326 | 7.3:+12862591 | MS.gene09868:CDS |
| GGTGGAATCGGTGGTGATTT+AGG | 0.312961 | 7.3:+12862513 | MS.gene09868:CDS |
| GGTGGTGTTGGTGGTGGAGC+TGG | 0.316689 | 7.3:+12862579 | MS.gene09868:CDS |
| GGAAACAATGGACTTCCTTT+TGG | 0.322482 | 7.3:+12862480 | MS.gene09868:CDS |
| TTGGGAGGACTTGGTGGTGT+TGG | 0.324253 | 7.3:+12862567 | MS.gene09868:CDS |
| GGTGGTGTTGGAGGTGGTGT+TGG | 0.331290 | 7.3:+12862603 | MS.gene09868:CDS |
| GCTGGTGGTTATTCAGGAAT+AGG | 0.341133 | 7.3:+12862459 | MS.gene09868:CDS |
| GTTGGAGGTGGTGTTGGAAC+TGG | 0.344227 | 7.3:+12862609 | MS.gene09868:CDS |
| CAAATGTCATGAAGTTCTTC+TGG | 0.354882 | 7.3:-12862437 | None:intergenic |
| GGACTTGGTGGTGTTGGTGG+TGG | 0.355251 | 7.3:+12862573 | MS.gene09868:CDS |
| GGAGGACTTGGTGGTGTTGG+TGG | 0.372674 | 7.3:+12862570 | MS.gene09868:CDS |
| GCTGGTGGTGGTGTTGGAGG+TGG | 0.373109 | 7.3:+12862597 | MS.gene09868:CDS |
| AAGAACTTCATGACATTTGC+TGG | 0.375624 | 7.3:+12862441 | MS.gene09868:CDS |
| GGAGCTGGTGGTGGTGTTGG+AGG | 0.379910 | 7.3:+12862594 | MS.gene09868:CDS |
| GTTGGTGGTGGAGCTGGTGG+TGG | 0.385227 | 7.3:+12862585 | MS.gene09868:CDS |
| GGTGGTGTTGGAACTGGTGT+TGG | 0.385912 | 7.3:+12862615 | MS.gene09868:CDS |
| GGTGGAATTGGAGGTGGAAT+CGG | 0.386910 | 7.3:+12862501 | MS.gene09868:CDS |
| GGTGGTGGCTTAGGTGGACT+TGG | 0.416312 | 7.3:+12862540 | MS.gene09868:CDS |
| GGTGGTTTGGGAGGACTTGG+TGG | 0.435056 | 7.3:+12862561 | MS.gene09868:CDS |
| GGTGGTGGTGGTGGCTTAGG+TGG | 0.452079 | 7.3:+12862534 | MS.gene09868:CDS |
| GGTGTTGGTGGTGGAGCTGG+TGG | 0.455563 | 7.3:+12862582 | MS.gene09868:CDS |
| GGTGTTGGAACTGGTGTTGG+TGG | 0.458137 | 7.3:+12862618 | MS.gene09868:CDS |
| AAAGTGGTGTATGTTGTTGT+TGG | 0.463389 | 7.3:+12862350 | MS.gene09868:CDS |
| GGTGGCTTAGGTGGACTTGG+TGG | 0.480958 | 7.3:+12862543 | MS.gene09868:CDS |
| GGTGGACTTGGTGGTTTGGG+AGG | 0.499205 | 7.3:+12862552 | MS.gene09868:CDS |
| GGTGATTTAGGTGGTGGTGG+TGG | 0.511257 | 7.3:+12862525 | MS.gene09868:CDS |
| GTTGGTTCTTGCTCTTGTTG+TGG | 0.516173 | 7.3:+12862368 | MS.gene09868:CDS |
| ACCAACTGATGCTGGTTTGA+AGG | 0.518003 | 7.3:+12862413 | MS.gene09868:CDS |
| GAATATTGATCTTAAGGGAA+AGG | 0.526279 | 7.3:-12862648 | None:intergenic |
| ATCGGTGGTGATTTAGGTGG+TGG | 0.527781 | 7.3:+12862519 | MS.gene09868:CDS |
| CCACCTCCAATTCCACCAAA+AGG | 0.542402 | 7.3:-12862495 | None:intergenic |
| GGAATTGGAGGTGGAATCGG+TGG | 0.552447 | 7.3:+12862504 | MS.gene09868:CDS |
| GGTGGTGATTTAGGTGGTGG+TGG | 0.559090 | 7.3:+12862522 | MS.gene09868:CDS |
| AGAAATGTACCAACTGATGC+TGG | 0.575990 | 7.3:+12862405 | MS.gene09868:CDS |
| TATTCAGGAATAGGAAACAA+TGG | 0.605549 | 7.3:+12862468 | MS.gene09868:CDS |
| GGAATCGGTGGTGATTTAGG+TGG | 0.612801 | 7.3:+12862516 | MS.gene09868:CDS |
| GAGTGAGAGATATGGCAAAG+TGG | 0.614546 | 7.3:+12862334 | None:intergenic |
| AACTTCATGACATTTGCTGG+TGG | 0.621619 | 7.3:+12862444 | MS.gene09868:CDS |
| TCCTTCAAACCAGCATCAGT+TGG | 0.622405 | 7.3:-12862414 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTCAGGAATAGGAAACAA+TGG | + | chr7.3:12862468-12862487 | MS.gene09868:CDS | 30.0% | |
| AAAGTGGTGTATGTTGTTGT+TGG | + | chr7.3:12862350-12862369 | MS.gene09868:CDS | 35.0% | |
| AAGAACTTCATGACATTTGC+TGG | + | chr7.3:12862441-12862460 | MS.gene09868:CDS | 35.0% | |
| CAAATGTCATGAAGTTCTTC+TGG | - | chr7.3:12862440-12862459 | None:intergenic | 35.0% | |
| AGAAATGTACCAACTGATGC+TGG | + | chr7.3:12862405-12862424 | MS.gene09868:CDS | 40.0% | |
| ! | AACTTCATGACATTTGCTGG+TGG | + | chr7.3:12862444-12862463 | MS.gene09868:CDS | 40.0% |
| ! | ACATTTGCTGGTGGTTATTC+AGG | + | chr7.3:12862453-12862472 | MS.gene09868:CDS | 40.0% |
| !! | AACAATGGACTTCCTTTTGG+TGG | + | chr7.3:12862483-12862502 | MS.gene09868:CDS | 40.0% |
| !! | GGAAACAATGGACTTCCTTT+TGG | + | chr7.3:12862480-12862499 | MS.gene09868:CDS | 40.0% |
| GCTGGTGGTTATTCAGGAAT+AGG | + | chr7.3:12862459-12862478 | MS.gene09868:CDS | 45.0% | |
| TCCTTCAAACCAGCATCAGT+TGG | - | chr7.3:12862417-12862436 | None:intergenic | 45.0% | |
| ! | CTTCCTTTTGGTGGAATTGG+AGG | + | chr7.3:12862492-12862511 | MS.gene09868:CDS | 45.0% |
| ! | GGACTTCCTTTTGGTGGAAT+TGG | + | chr7.3:12862489-12862508 | MS.gene09868:CDS | 45.0% |
| ! | GTTGGTTCTTGCTCTTGTTG+TGG | + | chr7.3:12862368-12862387 | MS.gene09868:CDS | 45.0% |
| !! | ACCAACTGATGCTGGTTTGA+AGG | + | chr7.3:12862413-12862432 | MS.gene09868:CDS | 45.0% |
| !! | TTAGGTGGACTTGGTGGTTT+GGG | + | chr7.3:12862549-12862568 | MS.gene09868:CDS | 45.0% |
| ATCGGTGGTGATTTAGGTGG+TGG | + | chr7.3:12862519-12862538 | MS.gene09868:CDS | 50.0% | |
| CCACCTCCAATTCCACCAAA+AGG | - | chr7.3:12862498-12862517 | None:intergenic | 50.0% | |
| GGAATCGGTGGTGATTTAGG+TGG | + | chr7.3:12862516-12862535 | MS.gene09868:CDS | 50.0% | |
| GGTGGAATCGGTGGTGATTT+AGG | + | chr7.3:12862513-12862532 | MS.gene09868:CDS | 50.0% | |
| GGTGGAATTGGAGGTGGAAT+CGG | + | chr7.3:12862501-12862520 | MS.gene09868:CDS | 50.0% | |
| ! | CCTTTTGGTGGAATTGGAGG+TGG | + | chr7.3:12862495-12862514 | MS.gene09868:CDS | 50.0% |
| !! | CTTAGGTGGACTTGGTGGTT+TGG | + | chr7.3:12862548-12862567 | MS.gene09868:CDS | 50.0% |
| GGAATTGGAGGTGGAATCGG+TGG | + | chr7.3:12862504-12862523 | MS.gene09868:CDS | 55.0% | |
| ! | CTTGGTGGTTTGGGAGGACT+TGG | + | chr7.3:12862558-12862577 | MS.gene09868:CDS | 55.0% |
| ! | GGTGGTGATTTAGGTGGTGG+TGG | + | chr7.3:12862522-12862541 | MS.gene09868:CDS | 55.0% |
| ! | GGTGGTGTTGGAACTGGTGT+TGG | + | chr7.3:12862615-12862634 | MS.gene09868:CDS | 55.0% |
| ! | GGTGTTGGAACTGGTGTTGG+TGG | + | chr7.3:12862618-12862637 | MS.gene09868:CDS | 55.0% |
| ! | GTTGGAGGTGGTGTTGGAAC+TGG | + | chr7.3:12862609-12862628 | MS.gene09868:CDS | 55.0% |
| !! | GGTGATTTAGGTGGTGGTGG+TGG | + | chr7.3:12862525-12862544 | MS.gene09868:CDS | 55.0% |
| !! | TTAGGTGGTGGTGGTGGCTT+AGG | + | chr7.3:12862531-12862550 | MS.gene09868:CDS | 55.0% |
| !! | TTGGGAGGACTTGGTGGTGT+TGG | + | chr7.3:12862567-12862586 | MS.gene09868:CDS | 55.0% |
| ! | GGTGGCTTAGGTGGACTTGG+TGG | + | chr7.3:12862543-12862562 | MS.gene09868:CDS | 60.0% |
| ! | GGTGGTGGCTTAGGTGGACT+TGG | + | chr7.3:12862540-12862559 | MS.gene09868:CDS | 60.0% |
| ! | GGTGGTGTTGGAGGTGGTGT+TGG | + | chr7.3:12862603-12862622 | MS.gene09868:CDS | 60.0% |
| ! | GGTGGTTTGGGAGGACTTGG+TGG | + | chr7.3:12862561-12862580 | MS.gene09868:CDS | 60.0% |
| !! | GGACTTGGTGGTGTTGGTGG+TGG | + | chr7.3:12862573-12862592 | MS.gene09868:CDS | 60.0% |
| !! | GGAGGACTTGGTGGTGTTGG+TGG | + | chr7.3:12862570-12862589 | MS.gene09868:CDS | 60.0% |
| !! | GGTGGACTTGGTGGTTTGGG+AGG | + | chr7.3:12862552-12862571 | MS.gene09868:CDS | 60.0% |
| !! | GCTGGTGGTGGTGTTGGAGG+TGG | + | chr7.3:12862597-12862616 | MS.gene09868:CDS | 65.0% |
| !! | GGAGCTGGTGGTGGTGTTGG+AGG | + | chr7.3:12862594-12862613 | MS.gene09868:CDS | 65.0% |
| !! | GGTGGAGCTGGTGGTGGTGT+TGG | + | chr7.3:12862591-12862610 | MS.gene09868:CDS | 65.0% |
| !! | GGTGGTGGTGGTGGCTTAGG+TGG | + | chr7.3:12862534-12862553 | MS.gene09868:CDS | 65.0% |
| !! | GGTGGTGTTGGTGGTGGAGC+TGG | + | chr7.3:12862579-12862598 | MS.gene09868:CDS | 65.0% |
| !! | GGTGTTGGTGGTGGAGCTGG+TGG | + | chr7.3:12862582-12862601 | MS.gene09868:CDS | 65.0% |
| !! | GTTGGTGGTGGAGCTGGTGG+TGG | + | chr7.3:12862585-12862604 | MS.gene09868:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 12862345 | 12862659 | 12862345 | ID=MS.gene09868 |
| chr7.3 | mRNA | 12862345 | 12862659 | 12862345 | ID=MS.gene09868.t1;Parent=MS.gene09868 |
| chr7.3 | exon | 12862345 | 12862659 | 12862345 | ID=MS.gene09868.t1.exon1;Parent=MS.gene09868.t1 |
| chr7.3 | CDS | 12862345 | 12862659 | 12862345 | ID=cds.MS.gene09868.t1;Parent=MS.gene09868.t1 |
| Gene Sequence |
| Protein sequence |
