AlfalfaGEDB | MS.gene20436

M. sativa cultivar XinJiangDaYe / MS.gene20436

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20436.t1	XP_003618493.2	94.3	123	3	3	1	120	189	310	4.50E-52	213.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20436.t1	Q9FIW9	76.6	64	15	0	32	95	199	262	6.4e-23	108.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20436.t1	G7KKI2	94.3	123	3	3	1	120	189	310	3.2e-52	213.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049351	MS.gene20436	0.804807	1.81E-49	-1.69E-46
MS.gene050432	MS.gene20436	0.813301	2.74E-51	-1.69E-46
MS.gene051255	MS.gene20436	0.81856	1.84E-52	-1.69E-46
MS.gene051877	MS.gene20436	0.813314	2.72E-51	-1.69E-46
MS.gene052481	MS.gene20436	0.803793	2.94E-49	-1.69E-46
MS.gene052485	MS.gene20436	0.805085	1.58E-49	-1.69E-46
MS.gene055277	MS.gene20436	0.814901	1.22E-51	-1.69E-46
MS.gene056452	MS.gene20436	0.804065	2.58E-49	-1.69E-46
MS.gene056942	MS.gene20436	0.800792	1.22E-48	-1.69E-46
MS.gene057011	MS.gene20436	0.808301	3.31E-50	-1.69E-46
MS.gene058323	MS.gene20436	0.81707	3.99E-52	-1.69E-46
MS.gene058660	MS.gene20436	0.808291	3.33E-50	-1.69E-46
MS.gene058840	MS.gene20436	-0.811527	6.70E-51	-1.69E-46
MS.gene059530	MS.gene20436	0.808247	3.40E-50	-1.69E-46
MS.gene060837	MS.gene20436	0.822608	2.16E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20436.t1	MTR_6g012280	94.309	123	3	3	1	120	189	310	1.54e-77	232
MS.gene20436.t1	MTR_7g010300	64.286	98	26	2	1	95	191	282	7.29e-37	128
MS.gene20436.t1	MTR_4g134580	60.526	76	30	0	33	108	284	359	1.13e-27	104
MS.gene20436.t1	MTR_8g467340	66.176	68	23	0	33	100	271	338	4.19e-27	103
MS.gene20436.t1	MTR_5g032600	69.841	63	19	0	33	95	209	271	2.35e-26	99.4
MS.gene20436.t1	MTR_3g100650	48.421	95	49	0	1	95	179	273	3.29e-26	99.0
MS.gene20436.t1	MTR_5g065440	66.667	63	21	0	33	95	205	267	1.62e-25	97.1
MS.gene20436.t1	MTR_5g020510	47.253	91	40	2	13	95	205	295	4.44e-24	93.6
MS.gene20436.t1	MTR_1g077020	58.824	68	27	1	33	99	124	191	5.98e-23	88.6
MS.gene20436.t1	MTR_2g076780	59.375	64	26	0	32	95	173	236	6.74e-22	87.0
MS.gene20436.t1	MTR_8g069380	59.375	64	26	0	32	95	51	114	1.56e-21	82.8
MS.gene20436.t1	MTR_6g073013	59.016	61	24	1	33	92	101	161	3.36e-21	83.6
MS.gene20436.t1	MTR_8g051480	59.375	64	25	1	32	95	66	128	1.27e-20	80.9
MS.gene20436.t1	MTR_8g069080	56.716	67	28	1	30	95	201	267	1.28e-19	81.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20436.t1	AT3G28920	61.957	92	35	0	1	92	160	251	1.02e-33	119
MS.gene20436.t1	AT5G39760	76.562	64	15	0	32	95	199	262	2.17e-31	113
MS.gene20436.t1	AT5G15210	59.551	89	23	2	1	89	160	235	6.14e-29	105
MS.gene20436.t1	AT4G24660	63.889	72	25	1	24	95	149	219	8.97e-26	96.7
MS.gene20436.t1	AT2G02540	63.492	63	23	0	33	95	222	284	3.75e-25	97.1
MS.gene20436.t1	AT2G02540	63.492	63	23	0	33	95	222	284	3.75e-25	97.1
MS.gene20436.t1	AT5G65410	50.575	87	41	1	17	101	173	259	8.34e-25	95.5
MS.gene20436.t1	AT1G75240	64.516	62	22	0	29	90	236	297	1.09e-24	95.5
MS.gene20436.t1	AT4G24660	60.526	76	26	2	17	92	145	216	4.62e-24	92.4
MS.gene20436.t1	AT1G69600	52.174	92	42	1	1	92	126	215	2.12e-23	90.9
MS.gene20436.t1	AT1G14440	55.844	77	34	0	19	95	204	280	2.39e-23	92.0
MS.gene20436.t1	AT1G14440	55.844	77	34	0	19	95	204	280	2.39e-23	92.0
MS.gene20436.t1	AT2G18350	60.317	63	25	0	33	95	198	260	1.63e-22	89.0
MS.gene20436.t1	AT3G50890	57.895	57	24	0	38	94	186	242	2.00e-19	80.5
MS.gene20436.t1	AT5G60480	59.649	57	23	0	33	89	147	203	8.34e-18	75.9
MS.gene20436.t1	AT5G42780	45.763	59	32	0	34	92	180	238	2.80e-14	67.0

Find 32 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATCTTCAGATCATGTTAA+TGG	0.215089	6.3:-6700408	MS.gene20436:CDS
ATAGAAGTGTTCTCAAAGTT+TGG	0.303184	6.3:-6700467	MS.gene20436:CDS
GCCGCCGTGGACGGTGGCTC+CGG	0.363036	6.3:-6700366	MS.gene20436:CDS
GCAGAAGAGAGATGAAGAAA+TGG	0.377517	6.3:-6700520	MS.gene20436:CDS
CATAATAATAAGAACACTTT+GGG	0.393395	6.3:-6700441	MS.gene20436:CDS
GCACTCTCCGGCGGCGGAGT+TGG	0.428986	6.3:-6700687	MS.gene20436:CDS
ACCGTCCACGGCGGCGTTCA+CGG	0.433426	6.3:+6700374	None:intergenic
CATGAATTTGCTGATAAAGT+TGG	0.436563	6.3:-6700552	MS.gene20436:CDS
GTTAAACCTAAGTTCACCTA+TGG	0.442479	6.3:-6700631	MS.gene20436:CDS
TTGTTCCCATAGGTGAACTT+AGG	0.444104	6.3:+6700625	None:intergenic
CTCCGCCGCCGGAGAGTGCT+AGG	0.449288	6.3:+6700691	None:intergenic
GCATAATAATAAGAACACTT+TGG	0.453675	6.3:-6700442	MS.gene20436:CDS
ATAGGTGAACTTAGGTTTAA+CGG	0.456911	6.3:+6700633	None:intergenic
GCCGTGAACGCCGCCGTGGA+CGG	0.475419	6.3:-6700375	MS.gene20436:CDS
GTGGACGGTGGCTCCGGTGG+TGG	0.484662	6.3:-6700360	MS.gene20436:CDS
CTCCTAGCACTCTCCGGCGG+CGG	0.489973	6.3:-6700693	MS.gene20436:CDS
ACCACCGGAGCCACCGTCCA+CGG	0.527788	6.3:+6700362	None:intergenic
CTCATTCTCACAACCACCAC+CGG	0.530426	6.3:+6700347	None:intergenic
ATGAATTTGCTGATAAAGTT+GGG	0.532314	6.3:-6700551	MS.gene20436:CDS
CGCCGCCGTGAACGCCGCCG+TGG	0.548960	6.3:-6700379	MS.gene20436:CDS
AGAGATGAAGAAATGGTGAA+TGG	0.553318	6.3:-6700513	MS.gene20436:CDS
GTGAACGCCGCCGTGGACGG+TGG	0.560193	6.3:-6700372	MS.gene20436:CDS
AAAATTCACACAAGAACAGA+AGG	0.564253	6.3:-6700583	MS.gene20436:CDS
GCCGTGGACGGTGGCTCCGG+TGG	0.577048	6.3:-6700363	MS.gene20436:CDS
CATATGCTCCTAGCACTCTC+CGG	0.577763	6.3:-6700699	MS.gene20436:CDS
AGAGAGACCAACTCCGCCGC+CGG	0.587710	6.3:+6700680	None:intergenic
AGTTGGTCTCTCTATCCCAC+CGG	0.613344	6.3:-6700670	MS.gene20436:CDS
TTAAACCTAAGTTCACCTAT+GGG	0.614933	6.3:-6700630	MS.gene20436:CDS
GTCCACGGCGGCGTTCACGG+CGG	0.666295	6.3:+6700377	None:intergenic
AATTTGCTGATAAAGTTGGG+TGG	0.672155	6.3:-6700548	MS.gene20436:CDS
ACCGGAGCCACCGTCCACGG+CGG	0.706919	6.3:+6700365	None:intergenic
ATGCTCCTAGCACTCTCCGG+CGG	0.721377	6.3:-6700696	MS.gene20436:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATAATAATAAGAACACTTT+GGG	-	chr6.3:6700613-6700632	MS.gene20436:CDS	20.0%
!	GCATAATAATAAGAACACTT+TGG	-	chr6.3:6700612-6700631	MS.gene20436:CDS	25.0%
!!!	ATGAATTTGCTGATAAAGTT+GGG	-	chr6.3:6700503-6700522	MS.gene20436:CDS	25.0%
	AAAATTCACACAAGAACAGA+AGG	-	chr6.3:6700471-6700490	MS.gene20436:CDS	30.0%
	ATAGGTGAACTTAGGTTTAA+CGG	+	chr6.3:6700424-6700443	None:intergenic	30.0%
	TCATCTTCAGATCATGTTAA+TGG	-	chr6.3:6700646-6700665	MS.gene20436:CDS	30.0%
	TTAAACCTAAGTTCACCTAT+GGG	-	chr6.3:6700424-6700443	MS.gene20436:CDS	30.0%
!	AATGGTTTTTGCAATGAAGT+TGG	-	chr6.3:6700559-6700578	MS.gene20436:CDS	30.0%
!!	ATAGAAGTGTTCTCAAAGTT+TGG	-	chr6.3:6700587-6700606	MS.gene20436:CDS	30.0%
!!	CATGAATTTGCTGATAAAGT+TGG	-	chr6.3:6700502-6700521	MS.gene20436:CDS	30.0%
	AGAGATGAAGAAATGGTGAA+TGG	-	chr6.3:6700541-6700560	MS.gene20436:CDS	35.0%
	GTTAAACCTAAGTTCACCTA+TGG	-	chr6.3:6700423-6700442	MS.gene20436:CDS	35.0%
!	CTTTTTCTACTTGTTCCCAT+AGG	+	chr6.3:6700442-6700461	None:intergenic	35.0%
!!	AATTTGCTGATAAAGTTGGG+TGG	-	chr6.3:6700506-6700525	MS.gene20436:CDS	35.0%
	GCAGAAGAGAGATGAAGAAA+TGG	-	chr6.3:6700534-6700553	MS.gene20436:CDS	40.0%
	TTGTTCCCATAGGTGAACTT+AGG	+	chr6.3:6700432-6700451	None:intergenic	40.0%
!	AACGGTGCAGCAGTGTTTTC+CGG	+	chr6.3:6700406-6700425	None:intergenic	50.0%
!	AGTTGGTCTCTCTATCCCAC+CGG	-	chr6.3:6700384-6700403	MS.gene20436:CDS	50.0%
!	GTGCAGCAGTGTTTTCCGGT+GGG	+	chr6.3:6700402-6700421	None:intergenic	55.0%
	ATGCTCCTAGCACTCTCCGG+CGG	-	chr6.3:6700358-6700377	MS.gene20436:CDS	60.0%
!	GGTGCAGCAGTGTTTTCCGG+TGG	+	chr6.3:6700403-6700422	None:intergenic	60.0%
	AGAGAGACCAACTCCGCCGC+CGG	+	chr6.3:6700377-6700396	None:intergenic	65.0%
	ACCACCGGAGCCACCGTCCA+CGG	+	chr6.3:6700695-6700714	None:intergenic	70.0%
	ACCGTCCACGGCGGCGTTCA+CGG	+	chr6.3:6700683-6700702	None:intergenic	70.0%
	CTCCTAGCACTCTCCGGCGG+CGG	-	chr6.3:6700361-6700380	MS.gene20436:CDS	70.0%
	ACCGGAGCCACCGTCCACGG+CGG	+	chr6.3:6700692-6700711	None:intergenic	75.0%
	GCACTCTCCGGCGGCGGAGT+TGG	-	chr6.3:6700367-6700386	MS.gene20436:CDS	75.0%
	GCCGTGAACGCCGCCGTGGA+CGG	-	chr6.3:6700679-6700698	MS.gene20436:CDS	75.0%
	GTCCACGGCGGCGTTCACGG+CGG	+	chr6.3:6700680-6700699	None:intergenic	75.0%
	GTGAACGCCGCCGTGGACGG+TGG	-	chr6.3:6700682-6700701	MS.gene20436:CDS	75.0%
	GTGGACGGTGGCTCCGGTGG+TGG	-	chr6.3:6700694-6700713	MS.gene20436:CDS	75.0%
!!	CTCCGCCGCCGGAGAGTGCT+AGG	+	chr6.3:6700366-6700385	None:intergenic	75.0%
!	GCCGCCGTGGACGGTGGCTC+CGG	-	chr6.3:6700688-6700707	MS.gene20436:CDS	80.0%
!	GCCGTGGACGGTGGCTCCGG+TGG	-	chr6.3:6700691-6700710	MS.gene20436:CDS	80.0%
!	CGCCGCCGTGAACGCCGCCG+TGG	-	chr6.3:6700675-6700694	MS.gene20436:CDS	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	6700358	6700718	6700358	ID=MS.gene20436
chr6.3	mRNA	6700358	6700718	6700358	ID=MS.gene20436.t1;Parent=MS.gene20436
chr6.3	exon	6700358	6700718	6700358	ID=MS.gene20436.t1.exon1;Parent=MS.gene20436.t1
chr6.3	CDS	6700358	6700718	6700358	ID=cds.MS.gene20436.t1;Parent=MS.gene20436.t1

Gene Sequence	>MS.gene20436 ATGCTCCTAGCACTCTCCGGCGGCGGAGTTGGTCTCTCTATCCCACCGGAAAACACTGCTGCACCGTTAAACCTAAGTTCACCTATGGGAACAAGTAGAAAAAGATTCAGAACAAAATTCACACAAGAACAGAAGGAAAAGATGCATGAATTTGCTGATAAAGTTGGGTGGAAGATGCAGAAGAGAGATGAAGAAATGGTGAATGGTTTTTGCAATGAAGTTGGTGTTGATAGAAGTGTTCTCAAAGTTTGGATGCATAATAATAAGAACACTTTGGGAAGAAAATTATCATCTTCAGATCATGTTAATGGTGACAGCGCCGCCGTGAACGCCGCCGTGGACGGTGGCTCCGGTGGTGGTT
Protein sequence	>MS.gene20436.t1 MLLALSGGGVGLSIPPENTAAPLNLSSPMGTSRKRFRTKFTQEQKEKMHEFADKVGWKMQKRDEEMVNGFCNEVGVDRSVLKVWMHNNKNTLGRKLSSSDHVNGDSAAVNAAVDGGSGGG