AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20684

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20684.t1 MTR_1g088995 93.910 312 12 2 1 305 1 312 0.0 569
MS.gene20684.t1 MTR_1g088995 87.342 237 23 2 1 230 1 237 4.92e-139 392
MS.gene20684.t1 MTR_1g090330 34.146 287 178 4 29 305 17 302 7.90e-54 177
MS.gene20684.t1 MTR_1g090330 27.041 196 131 4 17 201 102 296 3.70e-14 72.0
MS.gene20684.t1 MTR_7g066690 36.545 301 163 6 16 298 7 297 5.55e-52 173
MS.gene20684.t1 MTR_7g066690 28.333 180 119 4 34 207 129 304 3.04e-13 69.3
MS.gene20684.t1 MTR_4g018750 34.516 310 168 6 10 300 4 297 3.25e-51 171
MS.gene20684.t1 MTR_3g100570 32.566 304 175 4 26 299 6 309 2.46e-49 166
MS.gene20684.t1 MTR_3g462140 34.915 295 169 7 21 299 10 297 1.13e-43 151
MS.gene20684.t1 MTR_5g032420 30.671 313 178 6 26 299 6 318 3.18e-41 145
MS.gene20684.t1 MTR_4g018750 33.333 276 149 6 10 266 4 263 7.44e-41 144
MS.gene20684.t1 MTR_8g068890 30.719 306 180 5 26 299 6 311 6.57e-38 137
MS.gene20684.t1 MTR_8g086070 28.571 287 180 8 26 299 21 295 2.12e-29 114
MS.gene20684.t1 MTR_4g057850 28.986 276 163 8 41 296 37 299 4.01e-22 94.7
MS.gene20684.t1 MTR_1g097890 29.740 269 163 8 33 299 59 303 3.46e-20 89.4
MS.gene20684.t1 MTR_1g097890 29.670 182 120 3 25 204 131 306 3.61e-12 66.2
MS.gene20684.t1 MTR_7g109135 29.568 301 185 10 17 299 14 305 1.20e-19 87.8
MS.gene20684.t1 MTR_2g436930 25.407 307 180 9 30 294 19 318 1.39e-19 87.8
MS.gene20684.t1 MTR_2g436930 26.966 178 106 5 117 274 10 183 7.19e-11 62.4
MS.gene20684.t1 MTR_8g076760 27.208 283 181 9 41 302 29 307 1.46e-19 87.4
MS.gene20684.t1 MTR_4g119770 25.723 311 180 10 30 296 18 321 6.00e-18 83.2
MS.gene20684.t1 MTR_5g030530 27.562 283 179 8 39 301 21 297 1.83e-17 81.3
MS.gene20684.t1 MTR_8g086070 29.224 219 124 8 9 210 2 206 1.91e-17 79.7
MS.gene20684.t1 MTR_8g092080 28.016 257 158 7 21 274 47 279 2.91e-17 81.3
MS.gene20684.t1 MTR_1g095780 26.429 280 169 8 30 298 529 782 3.98e-17 82.0
MS.gene20684.t1 MTR_7g084400 26.705 176 123 3 30 202 313 485 1.75e-16 80.1
MS.gene20684.t1 MTR_7g084400 25.773 291 194 8 23 303 210 488 3.98e-14 72.8
MS.gene20684.t1 MTR_8g096050 26.298 289 194 8 25 303 226 505 2.10e-16 79.7
MS.gene20684.t1 MTR_2g102010 26.592 267 175 7 20 274 11 268 2.92e-16 77.8
MS.gene20684.t1 MTR_7g109135 28.283 297 187 10 17 299 14 298 3.07e-16 78.2
MS.gene20684.t1 MTR_3g025800 25.000 332 201 11 5 298 8 329 4.77e-16 78.2
MS.gene20684.t1 MTR_5g055410 28.309 272 175 9 42 298 37 303 5.41e-16 77.8
MS.gene20684.t1 MTR_1g012030 25.556 270 190 6 34 299 147 409 6.13e-16 78.2
MS.gene20684.t1 MTR_1g012030 28.922 204 130 6 5 203 218 411 4.47e-15 75.5
MS.gene20684.t1 MTR_1g012030 25.683 183 127 3 119 299 136 311 2.66e-12 67.4
MS.gene20684.t1 MTR_4g114320 29.121 182 118 4 27 204 222 396 1.36e-15 77.0
MS.gene20684.t1 MTR_4g114320 25.185 270 191 5 34 299 131 393 8.36e-14 71.6
MS.gene20684.t1 MTR_4g114320 28.022 182 123 3 119 298 120 295 1.32e-11 65.1
MS.gene20684.t1 MTR_7g064980 24.306 288 197 7 30 299 29 313 1.44e-15 76.3
MS.gene20684.t1 MTR_7g064980 28.718 195 119 6 121 299 24 214 7.05e-13 68.6
MS.gene20684.t1 MTR_8g066975 27.046 281 183 7 39 301 21 297 1.66e-15 75.9
MS.gene20684.t1 MTR_3g098460 25.175 286 201 6 25 303 204 483 1.76e-15 77.0
MS.gene20684.t1 MTR_3g107510 28.022 182 119 4 27 203 220 394 1.82e-15 76.6
MS.gene20684.t1 MTR_3g107510 25.830 271 188 7 34 299 130 392 3.85e-15 75.5
MS.gene20684.t1 MTR_1g087840 28.938 273 174 8 42 299 35 302 2.27e-15 75.5
MS.gene20684.t1 MTR_8g027975 29.442 197 127 4 110 297 41 234 7.94e-15 74.3
MS.gene20684.t1 MTR_8g027975 27.749 191 115 4 35 202 163 353 3.66e-14 72.4
MS.gene20684.t1 MTR_7g109135 29.915 234 143 8 80 299 1 227 2.43e-14 71.6
MS.gene20684.t1 MTR_7g109135 32.184 174 109 5 133 299 35 206 3.57e-14 70.9
MS.gene20684.t1 MTR_4g012850 27.037 270 173 8 25 281 15 273 4.40e-14 71.6
MS.gene20684.t1 MTR_4g012850 27.329 161 111 3 42 196 126 286 8.84e-11 62.0
MS.gene20684.t1 MTR_4g094578 24.262 305 185 10 23 302 22 305 4.40e-14 72.0
MS.gene20684.t1 MTR_7g068340 23.308 266 191 5 38 299 99 355 4.89e-14 72.4
MS.gene20684.t1 MTR_3g104600 23.926 326 212 9 4 302 15 331 6.22e-14 71.6
MS.gene20684.t1 MTR_5g009500 27.513 189 116 4 35 202 150 338 1.64e-13 70.5
MS.gene20684.t1 MTR_5g009500 28.108 185 123 3 122 297 38 221 6.83e-13 68.6
MS.gene20684.t1 MTR_5g009500 21.725 313 211 7 21 303 33 341 1.83e-11 64.3
MS.gene20684.t1 MTR_6g033280 25.652 230 145 6 24 234 57 279 2.20e-13 70.1
MS.gene20684.t1 MTR_2g032610 25.436 287 184 10 10 274 15 293 3.60e-13 69.3
MS.gene20684.t1 MTR_5g073040 28.821 229 147 6 25 243 34 256 6.91e-13 68.6
MS.gene20684.t1 MTR_6g034515 24.911 281 174 10 41 298 82 348 1.82e-12 67.4
MS.gene20684.t1 MTR_4g088545 22.623 305 208 6 20 303 6 303 5.88e-12 65.5
MS.gene20684.t1 MTR_6g091800 23.158 190 134 3 121 298 18 207 6.87e-12 65.1
MS.gene20684.t1 MTR_6g091800 23.158 190 134 3 121 298 18 207 7.92e-12 64.7
MS.gene20684.t1 MTR_7g015870 25.521 192 109 2 36 204 136 316 1.09e-11 64.7
MS.gene20684.t1 MTR_4g119770 25.100 251 145 8 30 244 18 261 1.09e-11 64.3
MS.gene20684.t1 MTR_7g015870 25.397 189 107 2 36 201 132 309 4.99e-11 63.2
MS.gene20684.t1 MTR_6g015860 25.521 192 109 2 36 204 128 308 5.28e-11 62.8
MS.gene20684.t1 MTR_4g088545 28.415 183 112 5 29 196 14 192 6.81e-11 62.0
MS.gene20684.t1 MTR_4g088545 28.415 183 112 5 29 196 14 192 7.07e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20684.t1 AT4G03115 69.637 303 87 2 3 305 17 314 2.62e-149 421
MS.gene20684.t1 AT4G03115 69.637 303 87 2 3 305 49 346 1.17e-148 421
MS.gene20684.t1 AT4G03115 72.441 254 67 1 52 305 1 251 3.14e-131 373
MS.gene20684.t1 AT4G03115 72.803 239 62 1 67 305 8 243 1.67e-124 356
MS.gene20684.t1 AT4G03115 69.444 216 61 2 3 218 17 227 9.23e-100 292
MS.gene20684.t1 AT4G03115 70.093 214 59 2 3 216 17 225 1.85e-99 291
MS.gene20684.t1 AT3G54110 34.615 286 169 5 26 298 15 295 7.89e-49 165
MS.gene20684.t1 AT5G58970 34.812 293 161 8 26 299 16 297 1.56e-46 159
MS.gene20684.t1 AT5G58970 26.966 178 124 3 31 204 125 300 2.59e-14 72.4
MS.gene20684.t1 AT1G14140 31.379 290 183 4 29 305 19 305 2.51e-43 150
MS.gene20684.t1 AT4G24570 30.476 315 167 6 26 299 6 309 1.87e-42 149
MS.gene20684.t1 AT5G09470 30.211 331 171 6 23 297 3 329 3.55e-37 135
MS.gene20684.t1 AT2G22500 29.967 307 177 7 26 299 6 307 7.21e-34 126
MS.gene20684.t1 AT5G58970 34.167 240 134 7 26 246 16 250 2.88e-33 123
MS.gene20684.t1 AT5G19760 28.767 292 183 8 21 299 13 292 1.52e-29 114
MS.gene20684.t1 AT5G01340 30.272 294 166 9 27 296 17 295 4.83e-24 99.8
MS.gene20684.t1 AT5G66380 27.740 292 190 9 27 299 10 299 9.04e-20 88.2
MS.gene20684.t1 AT5G48970 26.384 307 178 10 31 294 27 328 2.12e-19 87.4
MS.gene20684.t1 AT3G53940 26.756 299 190 10 25 299 66 359 6.99e-19 86.3
MS.gene20684.t1 AT3G53940 27.962 211 132 5 102 297 53 258 4.18e-14 72.4
MS.gene20684.t1 AT2G26360 26.882 279 171 8 30 298 119 374 5.41e-18 84.0
MS.gene20684.t1 AT2G37890 25.084 299 193 9 23 299 42 331 1.01e-17 82.8
MS.gene20684.t1 AT1G07030 28.897 263 168 9 23 274 36 290 1.48e-17 82.0
MS.gene20684.t1 AT3G21390 26.198 313 172 11 30 294 45 346 3.42e-17 81.3
MS.gene20684.t1 AT3G21390 26.198 313 172 11 30 294 23 324 3.73e-17 80.9
MS.gene20684.t1 AT1G25380 28.090 267 175 9 42 294 37 300 4.13e-17 81.3
MS.gene20684.t1 AT1G25380 28.090 267 175 9 42 294 37 300 4.13e-17 81.3
MS.gene20684.t1 AT5G51050 30.114 176 118 2 30 202 322 495 5.02e-17 81.6
MS.gene20684.t1 AT5G51050 25.704 284 191 7 24 298 221 493 2.95e-15 76.3
MS.gene20684.t1 AT1G34065 25.623 281 184 7 21 299 70 327 1.83e-16 79.0
MS.gene20684.t1 AT1G34065 25.623 281 184 7 21 299 77 334 2.96e-16 78.6
MS.gene20684.t1 AT3G55640 25.694 288 187 9 30 297 42 322 5.02e-16 77.8
MS.gene20684.t1 AT3G55640 28.796 191 118 5 121 297 37 223 1.11e-14 73.9
MS.gene20684.t1 AT3G55640 25.694 288 187 9 30 297 42 322 5.02e-16 77.8
MS.gene20684.t1 AT3G55640 28.796 191 118 5 121 297 37 223 1.11e-14 73.9
MS.gene20684.t1 AT2G35800 25.368 272 167 8 30 290 549 795 5.26e-16 79.0
MS.gene20684.t1 AT2G47490 28.986 276 169 9 42 299 33 299 7.99e-16 77.0
MS.gene20684.t1 AT4G01100 26.131 199 121 4 27 202 99 294 9.86e-16 76.6
MS.gene20684.t1 AT4G01100 23.711 291 192 7 42 303 8 297 1.60e-11 64.3
MS.gene20684.t1 AT4G01100 26.131 199 121 4 27 202 163 358 1.34e-15 76.6
MS.gene20684.t1 AT4G01100 23.460 341 213 9 10 303 22 361 1.48e-12 67.8
MS.gene20684.t1 AT4G01100 26.131 199 121 4 27 202 149 344 1.79e-15 76.3
MS.gene20684.t1 AT4G01100 23.242 327 217 8 10 303 22 347 3.20e-13 69.7
MS.gene20684.t1 AT1G74240 25.559 313 185 10 22 297 32 333 1.79e-15 76.3
MS.gene20684.t1 AT1G74240 25.253 198 124 6 125 299 39 235 9.61e-11 62.4
MS.gene20684.t1 AT3G55640 28.796 191 118 5 121 297 37 223 4.38e-15 73.9
MS.gene20684.t1 AT3G55640 28.796 191 118 5 121 297 37 223 4.38e-15 73.9
MS.gene20684.t1 AT5G61810 28.249 177 119 4 30 202 160 332 4.70e-15 75.1
MS.gene20684.t1 AT4G39460 26.275 255 165 5 21 274 53 285 5.22e-15 74.7
MS.gene20684.t1 AT4G39460 26.275 255 165 5 21 274 53 285 5.22e-15 74.7
MS.gene20684.t1 AT4G39460 26.275 255 165 5 21 274 53 285 5.22e-15 74.7
MS.gene20684.t1 AT4G26180 21.451 317 207 8 9 297 4 306 6.20e-15 74.7
MS.gene20684.t1 AT5G61810 28.249 177 119 4 30 202 303 475 8.76e-15 74.7
MS.gene20684.t1 AT4G26180 22.957 257 168 6 9 244 4 251 1.03e-14 73.6
MS.gene20684.t1 AT5G46800 27.915 283 173 9 39 297 21 296 1.03e-14 73.6
MS.gene20684.t1 AT5G46800 26.154 195 128 3 121 299 7 201 1.81e-12 67.0
MS.gene20684.t1 AT5G46800 27.915 283 173 9 39 297 21 296 1.03e-14 73.6
MS.gene20684.t1 AT5G46800 26.154 195 128 3 121 299 7 201 1.81e-12 67.0
MS.gene20684.t1 AT5G46800 27.915 283 173 9 39 297 21 296 1.03e-14 73.6
MS.gene20684.t1 AT5G46800 26.154 195 128 3 121 299 7 201 1.81e-12 67.0
MS.gene20684.t1 AT1G79900 29.940 167 106 5 34 196 118 277 1.91e-14 72.8
MS.gene20684.t1 AT1G79900 26.295 251 158 7 41 281 31 264 3.71e-14 72.0
MS.gene20684.t1 AT4G32400 25.735 272 187 8 34 299 122 384 1.96e-14 73.6
MS.gene20684.t1 AT4G32400 27.586 174 115 4 37 204 219 387 2.87e-13 70.1
MS.gene20684.t1 AT3G53940 27.962 211 132 5 102 297 53 258 2.59e-14 72.4
MS.gene20684.t1 AT5G07320 27.011 174 121 3 30 200 304 474 1.24e-13 71.6
MS.gene20684.t1 AT1G74240 25.850 294 170 10 41 297 3 285 1.84e-13 70.1
MS.gene20684.t1 AT2G30160 26.667 255 168 8 34 274 46 295 2.49e-13 69.7
MS.gene20684.t1 AT5G01500 25.830 271 178 7 38 299 131 387 5.95e-13 69.3
MS.gene20684.t1 AT5G48970 24.427 262 162 8 64 294 1 257 5.99e-13 68.2
MS.gene20684.t1 AT5G48970 26.570 207 123 6 26 205 64 268 9.49e-11 61.6
MS.gene20684.t1 AT3G51870 23.970 267 185 7 38 296 103 359 1.51e-12 67.8
MS.gene20684.t1 AT1G14560 24.286 280 183 10 42 297 43 317 3.30e-12 66.6
MS.gene20684.t1 AT2G39970 24.262 305 188 7 39 302 21 323 3.62e-12 66.6
MS.gene20684.t1 AT3G48850 26.423 246 152 7 25 251 66 301 8.22e-12 65.5
MS.gene20684.t1 AT3G20240 21.886 297 203 6 24 299 50 338 6.38e-11 62.8
MS.gene20684.t1 AT5G14040 25.346 217 144 6 31 234 87 298 7.35e-11 62.8

Find 86 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGACCTAAGTCTTTATATC+TGG 0.241224 1.2:+66872397 MS.gene20684:CDS
TATCCATGGGAGCAGTTATA+AGG 0.254065 1.2:-66873824 None:intergenic
TTAACAAGGTCAGTTGTTTA+TGG 0.264230 1.2:+66872434 MS.gene20684:CDS
TGAACTACCATTCCACTTAA+AGG 0.268699 1.2:-66872264 None:intergenic
GCAGCTGCTCTGGCCATGGC+AGG 0.301169 1.2:-66872784 None:intergenic
CATGCATGTGATCTTGCTTT+TGG 0.305682 1.2:+66872491 MS.gene20684:CDS
TTTGCAAATGAATTCAGATA+TGG 0.306923 1.2:+66872684 MS.gene20684:CDS
TGCAGTCAAGGCAGCTGCTC+TGG 0.336171 1.2:-66872794 None:intergenic
TATAACTGCTCCCATGGATA+TGG 0.336980 1.2:+66873827 MS.gene20684:CDS
GCTGGGAATTCCTCACTTCC+AGG 0.338797 1.2:+66870921 MS.gene20684:CDS
CAGGAGATGAGAAGCAGAAT+TGG 0.368865 1.2:+66871054 MS.gene20684:intron
TACAGCTCGAGCACAGTTGC+AGG 0.385585 1.2:+66873789 MS.gene20684:intron
CACCCTTTGTAAAGTGCTCT+TGG 0.386829 1.2:-66874234 None:intergenic
ATTGCTTCGGGAATGTTTGC+TGG 0.424631 1.2:+66872536 MS.gene20684:CDS
CTTGAGAACTTCCATTGGAT+TGG 0.428326 1.2:-66872579 None:intergenic
GTCCTCCGGAGCTCTAAAAT+TGG 0.430225 1.2:-66872718 None:intergenic
TTCACAATATTTGCAAGATT+AGG 0.434193 1.2:+66874355 MS.gene20684:CDS
CAGCTGCTCTGGCCATGGCA+GGG 0.435913 1.2:-66872783 None:intergenic
GCTAAGGAAAATTGCTGGCT+TGG 0.440588 1.2:+66874414 MS.gene20684:CDS
TGCCTTGACTGCATCTCAGC+TGG 0.442838 1.2:+66872804 MS.gene20684:CDS
TTTATATATTCTACCACCCT+TGG 0.448676 1.2:-66873880 None:intergenic
AAGATCACATGCATGCTTAG+AGG 0.450159 1.2:-66872483 None:intergenic
GGACCTAAGTCTTTATATCT+GGG 0.451211 1.2:+66872398 MS.gene20684:CDS
TTCAATCACTTTGCAACAAG+TGG 0.457233 1.2:+66871098 MS.gene20684:CDS
CTCACCTTGAGAACTTCCAT+TGG 0.467534 1.2:-66872584 None:intergenic
CAAGGCAGCTGCTCTGGCCA+TGG 0.468272 1.2:-66872788 None:intergenic
ATCCATGGGAGCAGTTATAA+GGG 0.468553 1.2:-66873823 None:intergenic
TTGCAAATGAATTCAGATAT+GGG 0.469855 1.2:+66872685 MS.gene20684:CDS
GAGGAGCTAAGGAAAATTGC+TGG 0.470269 1.2:+66874409 MS.gene20684:CDS
AAGGAAAATTGCTGGCTTGG+AGG 0.471885 1.2:+66874417 MS.gene20684:CDS
GTTTATGGAGGACTACGCTT+GGG 0.480206 1.2:+66872449 MS.gene20684:CDS
AGATAGAACAAAATCATGGC+TGG 0.481050 1.2:+66870903 None:intergenic
GAGTCAAAGCTCTGTGGAAG+GGG 0.484393 1.2:+66872755 MS.gene20684:CDS
AGGAGATGAGAAGCAGAATT+GGG 0.486763 1.2:+66871055 MS.gene20684:intron
GGTCAGAAGGGTCCTTTAAG+TGG 0.489706 1.2:+66872252 MS.gene20684:CDS
TTATATTATGTGAGGAGCTA+AGG 0.489794 1.2:+66874398 MS.gene20684:CDS
GAAAACAGTGGAAGGGGAGT+TGG 0.490931 1.2:-66871078 None:intergenic
AAACCCAGATATAAAGACTT+AGG 0.506639 1.2:-66872401 None:intergenic
ACATTAGAACTTGACCTGCT+TGG 0.513520 1.2:-66872842 None:intergenic
AGGCTACAAATGCAACTTGT+TGG 0.521186 1.2:+66872231 MS.gene20684:CDS
GGAGTCAAAGCTCTGTGGAA+GGG 0.524389 1.2:+66872754 MS.gene20684:CDS
CACCCTTATAACTGCTCCCA+TGG 0.525209 1.2:+66873821 MS.gene20684:CDS
GGGAGTCAAAGCTCTGTGGA+AGG 0.530782 1.2:+66872753 MS.gene20684:CDS
ACATAATATAAATGTAATTG+TGG 0.532518 1.2:-66874386 None:intergenic
ATAAATGTAATTGTGGTTTG+TGG 0.535455 1.2:-66874379 None:intergenic
AGCCAGCTGAGATGCAGTCA+AGG 0.539944 1.2:-66872806 None:intergenic
AGATCACATGCATGCTTAGA+GGG 0.540217 1.2:-66872482 None:intergenic
GTGGTTTCAGGTCCTACTTA+CGG 0.540672 1.2:+66874206 MS.gene20684:intron
GAAGGGTCCTTTAAGTGGAA+TGG 0.541076 1.2:+66872257 MS.gene20684:CDS
TGTTTATGGAGGACTACGCT+TGG 0.543442 1.2:+66872448 MS.gene20684:CDS
GTCCAAGAGCACTTTACAAA+GGG 0.550724 1.2:+66874232 MS.gene20684:CDS
CATGTTGCAACGAGAATCCA+AGG 0.552643 1.2:+66873863 MS.gene20684:CDS
GTGATTGAAAACAGTGGAAG+GGG 0.553107 1.2:-66871084 None:intergenic
CAATCCAATGGAAGTTCTCA+AGG 0.555333 1.2:+66872580 MS.gene20684:CDS
ATGCAACTTGTTGGTCAGAA+GGG 0.561302 1.2:+66872240 MS.gene20684:CDS
CCTCTTCAGATATAGTCCTC+CGG 0.564864 1.2:-66872732 None:intergenic
AATGAATTCAGATATGGGAA+AGG 0.566621 1.2:+66872690 MS.gene20684:CDS
GATAGAACAAAATCATGGCT+GGG 0.570849 1.2:+66870904 None:intergenic
AAGTGATTGAAAACAGTGGA+AGG 0.571611 1.2:-66871086 None:intergenic
AATGCAACTTGTTGGTCAGA+AGG 0.572639 1.2:+66872239 MS.gene20684:CDS
TACTTATGATGAAACCAAGC+AGG 0.576672 1.2:+66872828 MS.gene20684:CDS
CCGGAGGACTATATCTGAAG+AGG 0.578111 1.2:+66872732 MS.gene20684:CDS
GTTTGACACCTGCTTTAACA+AGG 0.581556 1.2:+66872420 MS.gene20684:CDS
ACAACTGACCTTGTTAAAGC+AGG 0.582971 1.2:-66872428 None:intergenic
ATGAATTCAGATATGGGAAA+GGG 0.584092 1.2:+66872691 MS.gene20684:CDS
CTCTTGGACCCTCCGTAAGT+AGG 0.584497 1.2:-66874218 None:intergenic
ACAAGGTCAGTTGTTTATGG+AGG 0.600975 1.2:+66872437 MS.gene20684:CDS
AATTCAGATATGGGAAAGGG+TGG 0.601368 1.2:+66872694 MS.gene20684:CDS
ACATCAATTACTCACCCTCT+AGG 0.602482 1.2:+66871140 MS.gene20684:CDS
CGGAGGACTATATCTGAAGA+GGG 0.606672 1.2:+66872733 MS.gene20684:CDS
AAGTGGACATCTCTCAGAGA+AGG 0.608766 1.2:+66872965 MS.gene20684:CDS
AAAGTTGTAACCTGGAAGTG+AGG 0.612010 1.2:-66870931 None:intergenic
TCATGCAGATTCTAGTCAAG+TGG 0.616514 1.2:+66872948 MS.gene20684:intron
AATTACATTTATATTATGTG+AGG 0.620137 1.2:+66874390 MS.gene20684:CDS
AGTCAAAGCTCTGTGGAAGG+GGG 0.629539 1.2:+66872756 MS.gene20684:CDS
AACTGCATTCACCAATCCAA+TGG 0.630939 1.2:+66872568 MS.gene20684:CDS
AGTGATTGAAAACAGTGGAA+GGG 0.633104 1.2:-66871085 None:intergenic
GGTCCAAGAGCACTTTACAA+AGG 0.633377 1.2:+66874231 MS.gene20684:CDS
GTGCAGATGTCTTGAAAGTG+AGG 0.638032 1.2:+66872211 MS.gene20684:intron
AAAACAAGAACGAACCTAGA+GGG 0.638593 1.2:-66871154 None:intergenic
TTTCAGGTCCTACTTACGGA+GGG 0.639390 1.2:+66874210 MS.gene20684:intron
GTTTCAGGTCCTACTTACGG+AGG 0.644049 1.2:+66874209 MS.gene20684:intron
AAGAGGGAGTCAAAGCTCTG+TGG 0.649547 1.2:+66872749 MS.gene20684:CDS
TGCAAAGTGATTGAAAACAG+TGG 0.674194 1.2:-66871090 None:intergenic
TTGCAACGAGAATCCAAGGG+TGG 0.712682 1.2:+66873867 MS.gene20684:CDS
ATGTTGCAACGAGAATCCAA+GGG 0.732517 1.2:+66873864 MS.gene20684:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTATAGAACTTATA+AGG - chr1.2:66874040-66874059 None:intergenic 10.0%
!!! TTTTTTAAAAAAAAAATACC+TGG - chr1.2:66871747-66871766 None:intergenic 10.0%
!! AAACAAATTTAGAAAAACAA+TGG - chr1.2:66872917-66872936 None:intergenic 15.0%
!! AATTACATTTATATTATGTG+AGG + chr1.2:66874390-66874409 MS.gene20684:CDS 15.0%
!! ACATAATATAAATGTAATTG+TGG - chr1.2:66874389-66874408 None:intergenic 15.0%
!! GTTTAATTGAAAGTAAATTA+GGG - chr1.2:66872895-66872914 None:intergenic 15.0%
!! TTTCTAATAAAATACAACAA+GGG + chr1.2:66873503-66873522 MS.gene20684:intron 15.0%
!!! ATTTTATTCTTATTCTTTCT+GGG + chr1.2:66871968-66871987 MS.gene20684:intron 15.0%
!!! GTTTTGTATTATAATGTAAT+AGG - chr1.2:66872320-66872339 None:intergenic 15.0%
!!! TATTTTATTCTTATTCTTTC+TGG + chr1.2:66871967-66871986 MS.gene20684:intron 15.0%
!!! TCTTAATTATTAGCTTTTAA+TGG + chr1.2:66874090-66874109 MS.gene20684:intron 15.0%
!! AGACAATAATGTATCTTTAA+TGG - chr1.2:66871858-66871877 None:intergenic 20.0%
!! AGTTAATTGAAAACAGAAAT+AGG + chr1.2:66871406-66871425 MS.gene20684:intron 20.0%
!! CAATTAACTGAAATTGTTTA+TGG - chr1.2:66871395-66871414 None:intergenic 20.0%
!! CTTATTTCAAATGTAAGATT+AGG + chr1.2:66873593-66873612 MS.gene20684:intron 20.0%
!! CTTTCTAATAAAATACAACA+AGG + chr1.2:66873502-66873521 MS.gene20684:intron 20.0%
!! GGTTTAATTGAAAGTAAATT+AGG - chr1.2:66872896-66872915 None:intergenic 20.0%
!! GTATTATAATGTAATAGGTA+AGG - chr1.2:66872315-66872334 None:intergenic 20.0%
!! GTTGATTAGTATTAAAGTTT+AGG - chr1.2:66874311-66874330 None:intergenic 20.0%
!! TAAAGATGTTATCATTAAGT+TGG + chr1.2:66872071-66872090 MS.gene20684:intron 20.0%
!! TATACAAGTAAAAGATATGA+TGG - chr1.2:66872610-66872629 None:intergenic 20.0%
!! TCTTCTCAACATATTATTAA+AGG - chr1.2:66872114-66872133 None:intergenic 20.0%
!! TGAAAAATAATGAATGTATC+AGG + chr1.2:66871035-66871054 MS.gene20684:intron 20.0%
!!! AGCAATTTTAACTAAAACAT+TGG - chr1.2:66872522-66872541 None:intergenic 20.0%
!!! ATATCATTGTTTTTCATGAT+TGG - chr1.2:66874144-66874163 None:intergenic 20.0%
!!! GAGAAGAAACTATTTTATTT+GGG + chr1.2:66872127-66872146 MS.gene20684:intron 20.0%
!!! TATCATTGTTTTTCATGATT+GGG - chr1.2:66874143-66874162 None:intergenic 20.0%
!!! TGAGAAGAAACTATTTTATT+TGG + chr1.2:66872126-66872145 MS.gene20684:intron 20.0%
!!! TGTTTTAGTTAAAATTGCTT+CGG + chr1.2:66872523-66872542 MS.gene20684:CDS 20.0%
! ATAAATGTAATTGTGGTTTG+TGG - chr1.2:66874382-66874401 None:intergenic 25.0%
! ATAAGAACACAAATTCTTCA+CGG - chr1.2:66873455-66873474 None:intergenic 25.0%
! ATGTTATATGAAACAATGTC+AGG + chr1.2:66873386-66873405 MS.gene20684:intron 25.0%
! ATTACAATTACAACTTGTGA+TGG - chr1.2:66870999-66871018 None:intergenic 25.0%
! CAATTCTAACTGTTACAATA+AGG - chr1.2:66873532-66873551 None:intergenic 25.0%
! CTTCATTATTCTTCACTATA+TGG - chr1.2:66873119-66873138 None:intergenic 25.0%
! GGTGGTAGAATATATAAAAA+TGG + chr1.2:66873885-66873904 MS.gene20684:CDS 25.0%
! TAAAATAAAGAAGCAGAGAT+CGG - chr1.2:66873983-66874002 None:intergenic 25.0%
! TTAAGTGTGATGAAAAATGA+AGG + chr1.2:66872377-66872396 MS.gene20684:CDS 25.0%
! TTCACAATATTTGCAAGATT+AGG + chr1.2:66874355-66874374 MS.gene20684:CDS 25.0%
! TTGCAAATGAATTCAGATAT+GGG + chr1.2:66872685-66872704 MS.gene20684:CDS 25.0%
! TTTCATCTACATCTTATGTA+TGG + chr1.2:66872989-66873008 MS.gene20684:intron 25.0%
! TTTGCAAATGAATTCAGATA+TGG + chr1.2:66872684-66872703 MS.gene20684:CDS 25.0%
!! ATAGTTAGTTTGACATTAGT+TGG + chr1.2:66873330-66873349 MS.gene20684:intron 25.0%
!! CAATGATATATTTTCTGATC+TGG + chr1.2:66874155-66874174 MS.gene20684:intron 25.0%
!! GAAACAATGATTTTCAACAT+TGG + chr1.2:66873269-66873288 MS.gene20684:intron 25.0%
!! TAGTTAGTTTGACATTAGTT+GGG + chr1.2:66873331-66873350 MS.gene20684:intron 25.0%
!! TTTCTTCTTTAACTTTGACT+AGG + chr1.2:66874057-66874076 MS.gene20684:intron 25.0%
!!! ATTAGCTTTTAATGGAATGA+TGG + chr1.2:66874098-66874117 MS.gene20684:intron 25.0%
!!! GTATTTTATTAGAAAGCACA+AGG - chr1.2:66873498-66873517 None:intergenic 25.0%
!!! GTTTTAGTTAAAATTGCTTC+GGG + chr1.2:66872524-66872543 MS.gene20684:CDS 25.0%
!!! TCTTTAATGGACTAATTTGA+TGG - chr1.2:66871845-66871864 None:intergenic 25.0%
AAACCCAGATATAAAGACTT+AGG - chr1.2:66872404-66872423 None:intergenic 30.0%
AAGTGTTAGACTATATCTAG+AGG + chr1.2:66871320-66871339 MS.gene20684:intron 30.0%
AATGAATTCAGATATGGGAA+AGG + chr1.2:66872690-66872709 MS.gene20684:CDS 30.0%
AATTCATTTGCCATCACTTT+AGG - chr1.2:66871902-66871921 None:intergenic 30.0%
ATACCAGACATCAATCAAAT+TGG - chr1.2:66871218-66871237 None:intergenic 30.0%
ATGAATTCAGATATGGGAAA+GGG + chr1.2:66872691-66872710 MS.gene20684:CDS 30.0%
GATCTGGATTCACTTATTAA+GGG + chr1.2:66874171-66874190 MS.gene20684:intron 30.0%
GATGATTTATTGATACATCG+AGG + chr1.2:66871926-66871945 MS.gene20684:intron 30.0%
GTGCTAACAATTTATGTGTT+AGG + chr1.2:66871657-66871676 MS.gene20684:intron 30.0%
TAACAGTTAGAATTGTTGCT+AGG + chr1.2:66873537-66873556 MS.gene20684:intron 30.0%
TGATCTGGATTCACTTATTA+AGG + chr1.2:66874170-66874189 MS.gene20684:intron 30.0%
TTAACAAGGTCAGTTGTTTA+TGG + chr1.2:66872434-66872453 MS.gene20684:CDS 30.0%
TTATATTATGTGAGGAGCTA+AGG + chr1.2:66874398-66874417 MS.gene20684:CDS 30.0%
TTTATATATTCTACCACCCT+TGG - chr1.2:66873883-66873902 None:intergenic 30.0%
! ATCCATTTAGAATTCATTGC+AGG - chr1.2:66873626-66873645 None:intergenic 30.0%
! GTCAATTAAGCATTTTTCCA+GGG + chr1.2:66872344-66872363 MS.gene20684:intron 30.0%
! GTTTGGTATGCATTTTTACA+CGG + chr1.2:66874265-66874284 MS.gene20684:intron 30.0%
! TGTCAATTAAGCATTTTTCC+AGG + chr1.2:66872343-66872362 MS.gene20684:intron 30.0%
! TTTAGAGTAAACCCTCAATT+GGG + chr1.2:66871775-66871794 MS.gene20684:intron 30.0%
! TTTATTAGAAAGCACAAGGA+AGG - chr1.2:66873494-66873513 None:intergenic 30.0%
!! GATCAGTTATTTGTTTCTGT+TGG - chr1.2:66871484-66871503 None:intergenic 30.0%
!!! CTGATGATGGAAAATAGTTT+TGG - chr1.2:66873735-66873754 None:intergenic 30.0%
AAAAACAAGAACGAACCTAG+AGG - chr1.2:66871158-66871177 None:intergenic 35.0%
AAAACAAGAACGAACCTAGA+GGG - chr1.2:66871157-66871176 None:intergenic 35.0%
AAGTGATTGAAAACAGTGGA+AGG - chr1.2:66871089-66871108 None:intergenic 35.0%
ACACATAAATTGTTAGCACC+TGG - chr1.2:66871656-66871675 None:intergenic 35.0%
ACATCTGATGTACATTTGTC+AGG + chr1.2:66874329-66874348 MS.gene20684:intron 35.0%
AGATCGGTATAGAACAAACA+AGG - chr1.2:66873967-66873986 None:intergenic 35.0%
AGCCTGCAATGAATTCTAAA+TGG + chr1.2:66873621-66873640 MS.gene20684:intron 35.0%
AGGACCTAAGTCTTTATATC+TGG + chr1.2:66872397-66872416 MS.gene20684:CDS 35.0%
AGTCTGCATTAAGAGAGATA+CGG - chr1.2:66873037-66873056 None:intergenic 35.0%
AGTGATTGAAAACAGTGGAA+GGG - chr1.2:66871088-66871107 None:intergenic 35.0%
ATCAAGTGCTTAACAGTTGA+TGG + chr1.2:66871517-66871536 MS.gene20684:intron 35.0%
ATCTGATGTACATTTGTCAG+GGG + chr1.2:66874331-66874350 MS.gene20684:intron 35.0%
CAAAGATTGACCAAAATCAC+GGG + chr1.2:66873082-66873101 MS.gene20684:intron 35.0%
CACTTAAAAACAATTGTCCC+TGG - chr1.2:66872364-66872383 None:intergenic 35.0%
CATCTGATGTACATTTGTCA+GGG + chr1.2:66874330-66874349 MS.gene20684:intron 35.0%
CCAAAGATTGACCAAAATCA+CGG + chr1.2:66873081-66873100 MS.gene20684:intron 35.0%
CTAAGTCACATTTCTGATGA+TGG - chr1.2:66873748-66873767 None:intergenic 35.0%
CTATATCTAGAGGTTGGATT+TGG + chr1.2:66871330-66871349 MS.gene20684:intron 35.0%
GAAAGTAAATTAGGGAGATG+AGG - chr1.2:66872887-66872906 None:intergenic 35.0%
GAAGAAACAAAGTTGTAACC+TGG - chr1.2:66870942-66870961 None:intergenic 35.0%
GACTAAGATAGTCTAAATGC+AGG + chr1.2:66873361-66873380 MS.gene20684:intron 35.0%
GCAAAAATCAAGAACTGACA+CGG - chr1.2:66871823-66871842 None:intergenic 35.0%
GCAACACTTTGATCTTTAGA+GGG - chr1.2:66871801-66871820 None:intergenic 35.0%
GGACCTAAGTCTTTATATCT+GGG + chr1.2:66872398-66872417 MS.gene20684:CDS 35.0%
GTAATAGGTAAGGTCTAAAG+AGG - chr1.2:66872305-66872324 None:intergenic 35.0%
GTTAGACTATATCTAGAGGT+TGG + chr1.2:66871324-66871343 MS.gene20684:intron 35.0%
TACTTATGATGAAACCAAGC+AGG + chr1.2:66872828-66872847 MS.gene20684:CDS 35.0%
TGAACTACCATTCCACTTAA+AGG - chr1.2:66872267-66872286 None:intergenic 35.0%
TGACCAATTTGATTGATGTC+TGG + chr1.2:66871212-66871231 MS.gene20684:intron 35.0%
TGCAAAGTGATTGAAAACAG+TGG - chr1.2:66871093-66871112 None:intergenic 35.0%
TGTCTCCAAATTTGTCCTTA+TGG + chr1.2:66871873-66871892 MS.gene20684:intron 35.0%
TGTGTTCTTATCTGTAGTCA+GGG + chr1.2:66873464-66873483 MS.gene20684:intron 35.0%
TTCAATCACTTTGCAACAAG+TGG + chr1.2:66871098-66871117 MS.gene20684:CDS 35.0%
TTGACATTAGTTGGGAACTT+GGG + chr1.2:66873339-66873358 MS.gene20684:intron 35.0%
TTGTGTTCTTATCTGTAGTC+AGG + chr1.2:66873463-66873482 MS.gene20684:intron 35.0%
TTTGACATTAGTTGGGAACT+TGG + chr1.2:66873338-66873357 MS.gene20684:intron 35.0%
! ACATCGAGGATAGATCTTTT+AGG + chr1.2:66871940-66871959 MS.gene20684:intron 35.0%
! ATTTAGAATTCATTGCAGGC+TGG - chr1.2:66873622-66873641 None:intergenic 35.0%
! GATTGGTCCCAAAGATTAAT+CGG + chr1.2:66871706-66871725 MS.gene20684:intron 35.0%
! TGTGTGCTGATAGAAAACAT+AGG - chr1.2:66873678-66873697 None:intergenic 35.0%
!! CAAAGGGTGAGTTTTATGTT+TGG + chr1.2:66874248-66874267 MS.gene20684:intron 35.0%
!! CGTGATTTTGGTCAATCTTT+GGG - chr1.2:66873083-66873102 None:intergenic 35.0%
!! CTTTAGAGTAAACCCTCAAT+TGG + chr1.2:66871774-66871793 MS.gene20684:intron 35.0%
!! TAGAACAAACAAGGTACTGA+AGG - chr1.2:66873958-66873977 None:intergenic 35.0%
!!! CTCTGCTTCTTTATTTTACG+TGG + chr1.2:66873985-66874004 MS.gene20684:intron 35.0%
!!! TGGTTTTAACTGTGCATATC+AGG + chr1.2:66873905-66873924 MS.gene20684:CDS 35.0%
AAAACCATATTCCCGCAATC+AGG + chr1.2:66872657-66872676 MS.gene20684:intron 40.0%
AAAGTTGTAACCTGGAAGTG+AGG - chr1.2:66870934-66870953 None:intergenic 40.0%
AACTGCATTCACCAATCCAA+TGG + chr1.2:66872568-66872587 MS.gene20684:CDS 40.0%
AAGATCACATGCATGCTTAG+AGG - chr1.2:66872486-66872505 None:intergenic 40.0%
AATTCAGATATGGGAAAGGG+TGG + chr1.2:66872694-66872713 MS.gene20684:CDS 40.0%
ACAACTGACCTTGTTAAAGC+AGG - chr1.2:66872431-66872450 None:intergenic 40.0%
ACAAGGTCAGTTGTTTATGG+AGG + chr1.2:66872437-66872456 MS.gene20684:CDS 40.0%
ACATCAATTACTCACCCTCT+AGG + chr1.2:66871140-66871159 MS.gene20684:CDS 40.0%
ACATTAGAACTTGACCTGCT+TGG - chr1.2:66872845-66872864 None:intergenic 40.0%
ACCAAGGACCGATTAATCTT+TGG - chr1.2:66871717-66871736 None:intergenic 40.0%
AGAATTGTTGCTAGGAGACT+AGG + chr1.2:66873545-66873564 MS.gene20684:intron 40.0%
AGATCACATGCATGCTTAGA+GGG - chr1.2:66872485-66872504 None:intergenic 40.0%
AGGAGATGAGAAGCAGAATT+GGG + chr1.2:66871055-66871074 MS.gene20684:intron 40.0%
AGGCTACAAATGCAACTTGT+TGG + chr1.2:66872231-66872250 MS.gene20684:CDS 40.0%
AGGTAAGGTCTAAAGAGGAA+AGG - chr1.2:66872300-66872319 None:intergenic 40.0%
ATCCATGGGAGCAGTTATAA+GGG - chr1.2:66873826-66873845 None:intergenic 40.0%
ATCTGATGCTGACAAAGGAT+TGG - chr1.2:66873653-66873672 None:intergenic 40.0%
ATGTTGCAACGAGAATCCAA+GGG + chr1.2:66873864-66873883 MS.gene20684:CDS 40.0%
CAATCCAATGGAAGTTCTCA+AGG + chr1.2:66872580-66872599 MS.gene20684:CDS 40.0%
CCAAGGACCGATTAATCTTT+GGG - chr1.2:66871716-66871735 None:intergenic 40.0%
CTCCAAATTTGTCCTTATGG+TGG + chr1.2:66871876-66871895 MS.gene20684:intron 40.0%
CTTGAGAACTTCCATTGGAT+TGG - chr1.2:66872582-66872601 None:intergenic 40.0%
GAAACAATGTCAGGAGATGA+GGG + chr1.2:66873395-66873414 MS.gene20684:intron 40.0%
GCCTTAGGTCTATTAGTTGA+GGG + chr1.2:66873295-66873314 MS.gene20684:intron 40.0%
GGCAACACTTTGATCTTTAG+AGG - chr1.2:66871802-66871821 None:intergenic 40.0%
GTGATTGAAAACAGTGGAAG+GGG - chr1.2:66871087-66871106 None:intergenic 40.0%
GTTTGACACCTGCTTTAACA+AGG + chr1.2:66872420-66872439 MS.gene20684:CDS 40.0%
TATAACTGCTCCCATGGATA+TGG + chr1.2:66873827-66873846 MS.gene20684:CDS 40.0%
TATCCATGGGAGCAGTTATA+AGG - chr1.2:66873827-66873846 None:intergenic 40.0%
TCACTTTCAAGACATCTGCA+CGG - chr1.2:66872212-66872231 None:intergenic 40.0%
TCATGCAGATTCTAGTCAAG+TGG + chr1.2:66872948-66872967 MS.gene20684:intron 40.0%
TCCCTCAACTAATAGACCTA+AGG - chr1.2:66873299-66873318 None:intergenic 40.0%
TGAAACAATGTCAGGAGATG+AGG + chr1.2:66873394-66873413 MS.gene20684:intron 40.0%
TTCTGTTGGATCTACGACTT+GGG - chr1.2:66871470-66871489 None:intergenic 40.0%
TTTCTGTTGGATCTACGACT+TGG - chr1.2:66871471-66871490 None:intergenic 40.0%
! ACGTGTTTTCACCATATCCA+TGG - chr1.2:66873841-66873860 None:intergenic 40.0%
! ATGTTGTTTGACTAGCACAG+AGG + chr1.2:66871435-66871454 MS.gene20684:intron 40.0%
! CATGCATGTGATCTTGCTTT+TGG + chr1.2:66872491-66872510 MS.gene20684:CDS 40.0%
! CATTTTTCGTGTGTGGTTTC+AGG + chr1.2:66874194-66874213 MS.gene20684:intron 40.0%
! CGTGTTTTCACCATATCCAT+GGG - chr1.2:66873840-66873859 None:intergenic 40.0%
! TCTAGAGGTTGGATTTGGTT+TGG + chr1.2:66871335-66871354 MS.gene20684:intron 40.0%
!! AATGCAACTTGTTGGTCAGA+AGG + chr1.2:66872239-66872258 MS.gene20684:CDS 40.0%
!! AGGTTGGATTTGGTTTGGAT+GGG + chr1.2:66871340-66871359 MS.gene20684:intron 40.0%
!! ATGCAACTTGTTGGTCAGAA+GGG + chr1.2:66872240-66872259 MS.gene20684:CDS 40.0%
!! CCGTGATTTTGGTCAATCTT+TGG - chr1.2:66873084-66873103 None:intergenic 40.0%
!! GTCCAAGAGCACTTTACAAA+GGG + chr1.2:66874232-66874251 MS.gene20684:CDS 40.0%
!!! TTAAGGGCATTTTTCGTGTG+TGG + chr1.2:66874187-66874206 MS.gene20684:intron 40.0%
AACTGTGCTCGAGCTGTATA+AGG - chr1.2:66873787-66873806 None:intergenic 45.0%
AAGTGGACATCTCTCAGAGA+AGG + chr1.2:66872965-66872984 MS.gene20684:CDS 45.0%
AATACCTGGTATCCAGACCA+AGG - chr1.2:66871733-66871752 None:intergenic 45.0%
AGATTAATCGGTCCTTGGTC+TGG + chr1.2:66871718-66871737 MS.gene20684:intron 45.0%
AGGTGATCTGATGCTGACAA+AGG - chr1.2:66873658-66873677 None:intergenic 45.0%
AGTACTACCATTACCCCCAA+GGG + chr1.2:66871561-66871580 MS.gene20684:intron 45.0%
ATTGCTTCGGGAATGTTTGC+TGG + chr1.2:66872536-66872555 MS.gene20684:CDS 45.0%
CAGGAGATGAGAAGCAGAAT+TGG + chr1.2:66871054-66871073 MS.gene20684:intron 45.0%
CATCACTTTAGGCCACCATA+AGG - chr1.2:66871891-66871910 None:intergenic 45.0%
CATGTTGCAACGAGAATCCA+AGG + chr1.2:66873863-66873882 MS.gene20684:CDS 45.0%
CCCAAAGATTAATCGGTCCT+TGG + chr1.2:66871713-66871732 MS.gene20684:intron 45.0%
CCTCTTCAGATATAGTCCTC+CGG - chr1.2:66872735-66872754 None:intergenic 45.0%
CGCCTTAGGTCTATTAGTTG+AGG + chr1.2:66873294-66873313 MS.gene20684:intron 45.0%
CGGAGGACTATATCTGAAGA+GGG + chr1.2:66872733-66872752 MS.gene20684:CDS 45.0%
CTCACCTTGAGAACTTCCAT+TGG - chr1.2:66872587-66872606 None:intergenic 45.0%
CTTATGGTGGCCTAAAGTGA+TGG + chr1.2:66871889-66871908 MS.gene20684:intron 45.0%
GAAGGGTCCTTTAAGTGGAA+TGG + chr1.2:66872257-66872276 MS.gene20684:CDS 45.0%
GACTTAGAGATGAGCACACT+TGG - chr1.2:66871618-66871637 None:intergenic 45.0%
GAGGAGCTAAGGAAAATTGC+TGG + chr1.2:66874409-66874428 MS.gene20684:CDS 45.0%
GGCCACCATAAGGACAAATT+TGG - chr1.2:66871881-66871900 None:intergenic 45.0%
GGTAGTCACACTTCACATGT+GGG + chr1.2:66871989-66872008 MS.gene20684:intron 45.0%
GTCTCATGTTCGATTCTTCC+AGG + chr1.2:66871635-66871654 MS.gene20684:intron 45.0%
GTGCAGATGTCTTGAAAGTG+AGG + chr1.2:66872211-66872230 MS.gene20684:intron 45.0%
GTTTATGGAGGACTACGCTT+GGG + chr1.2:66872449-66872468 MS.gene20684:CDS 45.0%
TAGTACTACCATTACCCCCA+AGG + chr1.2:66871560-66871579 MS.gene20684:intron 45.0%
TGACTAGAATCTGCATGAGC+AGG - chr1.2:66872946-66872965 None:intergenic 45.0%
TGTTTATGGAGGACTACGCT+TGG + chr1.2:66872448-66872467 MS.gene20684:CDS 45.0%
TTAGAGATGAGCACACTTGG+AGG - chr1.2:66871615-66871634 None:intergenic 45.0%
TTTCAACATTGGCACGCCTT+AGG + chr1.2:66873280-66873299 MS.gene20684:intron 45.0%
TTTGCAAACGCACCTGATTG+CGG - chr1.2:66872672-66872691 None:intergenic 45.0%
! ATCTTTAGAGGGCCCAATTG+AGG - chr1.2:66871790-66871809 None:intergenic 45.0%
! CACCCTTTGTAAAGTGCTCT+TGG - chr1.2:66874237-66874256 None:intergenic 45.0%
! GAAGTTGTTGCCCGTGATTT+TGG - chr1.2:66873095-66873114 None:intergenic 45.0%
! GATGCTGACAAAGGATTGGT+CGG - chr1.2:66873649-66873668 None:intergenic 45.0%
! GCTAAGGAAAATTGCTGGCT+TGG + chr1.2:66874414-66874433 MS.gene20684:CDS 45.0%
! GTGGTTTCAGGTCCTACTTA+CGG + chr1.2:66874206-66874225 MS.gene20684:intron 45.0%
! TCTTTAGAGGGCCCAATTGA+GGG - chr1.2:66871789-66871808 None:intergenic 45.0%
! TTTCAGGTCCTACTTACGGA+GGG + chr1.2:66874210-66874229 MS.gene20684:intron 45.0%
!! AAGGAAAATTGCTGGCTTGG+AGG + chr1.2:66874417-66874436 MS.gene20684:CDS 45.0%
!! CAAGGTACTGAAGGTTTGAG+AGG - chr1.2:66873949-66873968 None:intergenic 45.0%
!! GAGGTTGGATTTGGTTTGGA+TGG + chr1.2:66871339-66871358 MS.gene20684:intron 45.0%
!! GGTCCAAGAGCACTTTACAA+AGG + chr1.2:66874231-66874250 MS.gene20684:CDS 45.0%
!! TGGATTTGGTTTGGATGGGA+GGG + chr1.2:66871344-66871363 MS.gene20684:intron 45.0%
!! TTGGATTTGGTTTGGATGGG+AGG + chr1.2:66871343-66871362 MS.gene20684:intron 45.0%
!!! GTGGACCAATTTTAGAGCTC+CGG + chr1.2:66872713-66872732 MS.gene20684:CDS 45.0%
!! AATATATATATATATAGTTA+GGG + chr1.2:66871238-66871257 MS.gene20684:intron 5.0%
!! TAATATATATATATATAGTT+AGG + chr1.2:66871237-66871256 MS.gene20684:intron 5.0%
!!! ATTAAATATAGATATTTTTA+AGG - chr1.2:66872176-66872195 None:intergenic 5.0%
AAACCAACCCTTGGGGGTAA+TGG - chr1.2:66871571-66871590 None:intergenic 50.0%
AAGAGGGAGTCAAAGCTCTG+TGG + chr1.2:66872749-66872768 MS.gene20684:CDS 50.0%
ACCAATCCAACCGTGCAGTA+CGG - chr1.2:66871693-66871712 None:intergenic 50.0%
AGTCAAAGCTCTGTGGAAGG+GGG + chr1.2:66872756-66872775 MS.gene20684:CDS 50.0%
ATGAGGGAAATCCGGGTCTT+CGG + chr1.2:66873411-66873430 MS.gene20684:intron 50.0%
CACATGTGGGCAGGGTAAAT+TGG + chr1.2:66872002-66872021 MS.gene20684:intron 50.0%
CACCCTTATAACTGCTCCCA+TGG + chr1.2:66873821-66873840 MS.gene20684:CDS 50.0%
CACGGTTACAATGACCTACC+TGG - chr1.2:66873437-66873456 None:intergenic 50.0%
CCGGAGGACTATATCTGAAG+AGG + chr1.2:66872732-66872751 MS.gene20684:CDS 50.0%
GAAAACAGTGGAAGGGGAGT+TGG - chr1.2:66871081-66871100 None:intergenic 50.0%
GAGTCAAAGCTCTGTGGAAG+GGG + chr1.2:66872755-66872774 MS.gene20684:CDS 50.0%
GGAGTCAAAGCTCTGTGGAA+GGG + chr1.2:66872754-66872773 MS.gene20684:CDS 50.0%
GGGTAGTCACACTTCACATG+TGG + chr1.2:66871988-66872007 MS.gene20684:intron 50.0%
GGTCAGAAGGGTCCTTTAAG+TGG + chr1.2:66872252-66872271 MS.gene20684:CDS 50.0%
GTCAGGAGATGAGGGAAATC+CGG + chr1.2:66873403-66873422 MS.gene20684:intron 50.0%
GTTGCTAGGAGACTAGGTCA+CGG + chr1.2:66873551-66873570 MS.gene20684:intron 50.0%
TCACACTTCACATGTGGGCA+GGG + chr1.2:66871994-66872013 MS.gene20684:intron 50.0%
TCAGGAGATGAGGGAAATCC+GGG + chr1.2:66873404-66873423 MS.gene20684:intron 50.0%
TTGCAAACGCACCTGATTGC+GGG - chr1.2:66872671-66872690 None:intergenic 50.0%
TTGCAACGAGAATCCAAGGG+TGG + chr1.2:66873867-66873886 MS.gene20684:CDS 50.0%
! GTTGGATCTACGACTTGGGA+GGG - chr1.2:66871466-66871485 None:intergenic 50.0%
! GTTTCAGGTCCTACTTACGG+AGG + chr1.2:66874209-66874228 MS.gene20684:intron 50.0%
! TGTTGGATCTACGACTTGGG+AGG - chr1.2:66871467-66871486 None:intergenic 50.0%
! TTGGATCTACGACTTGGGAG+GGG - chr1.2:66871465-66871484 None:intergenic 50.0%
!! ATTACCCCCAAGGGTTGGTT+TGG + chr1.2:66871570-66871589 MS.gene20684:intron 50.0%
!! GGATTTGGTTTGGATGGGAG+GGG + chr1.2:66871345-66871364 MS.gene20684:intron 50.0%
!! GGTTGGTTTGGTGGTGGTAT+AGG + chr1.2:66871582-66871601 MS.gene20684:intron 50.0%
!! GTCCTCCGGAGCTCTAAAAT+TGG - chr1.2:66872721-66872740 None:intergenic 50.0%
!!! GACCAATTTTAGAGCTCCGG+AGG + chr1.2:66872716-66872735 MS.gene20684:CDS 50.0%
AAAGCTCTGTGGAAGGGGGT+AGG + chr1.2:66872760-66872779 MS.gene20684:CDS 55.0%
ACCACCAAACCAACCCTTGG+GGG - chr1.2:66871577-66871596 None:intergenic 55.0%
ACCACCACCAAACCAACCCT+TGG - chr1.2:66871580-66871599 None:intergenic 55.0%
AGCCAGCTGAGATGCAGTCA+AGG - chr1.2:66872809-66872828 None:intergenic 55.0%
CACCACCAAACCAACCCTTG+GGG - chr1.2:66871578-66871597 None:intergenic 55.0%
CCACCACCAAACCAACCCTT+GGG - chr1.2:66871579-66871598 None:intergenic 55.0%
CGCACCTGATTGCGGGAATA+TGG - chr1.2:66872664-66872683 None:intergenic 55.0%
CTACCATTACCCCCAAGGGT+TGG + chr1.2:66871565-66871584 MS.gene20684:intron 55.0%
CTCTTGGACCCTCCGTAAGT+AGG - chr1.2:66874221-66874240 None:intergenic 55.0%
GCTGGGAATTCCTCACTTCC+AGG + chr1.2:66870921-66870940 MS.gene20684:CDS 55.0%
GGGAGTCAAAGCTCTGTGGA+AGG + chr1.2:66872753-66872772 MS.gene20684:CDS 55.0%
GTCACACTTCACATGTGGGC+AGG + chr1.2:66871993-66872012 MS.gene20684:intron 55.0%
TACAGCTCGAGCACAGTTGC+AGG + chr1.2:66873789-66873808 MS.gene20684:intron 55.0%
TGCCTTGACTGCATCTCAGC+TGG + chr1.2:66872804-66872823 MS.gene20684:CDS 55.0%
!! TTGGTTTGGATGGGAGGGGA+AGG + chr1.2:66871349-66871368 MS.gene20684:intron 55.0%
CCAGTCCATGCCGTACTGCA+CGG + chr1.2:66871680-66871699 MS.gene20684:intron 60.0%
CGGTCCTTGGTCTGGATACC+AGG + chr1.2:66871726-66871745 MS.gene20684:intron 60.0%
GCCGTACTGCACGGTTGGAT+TGG + chr1.2:66871689-66871708 MS.gene20684:intron 60.0%
TCCAACCGTGCAGTACGGCA+TGG - chr1.2:66871688-66871707 None:intergenic 60.0%
TCCATGCCGTACTGCACGGT+TGG + chr1.2:66871684-66871703 MS.gene20684:intron 60.0%
TGCAGTCAAGGCAGCTGCTC+TGG - chr1.2:66872797-66872816 None:intergenic 60.0%
!! ACCCCCAAGGGTTGGTTTGG+TGG + chr1.2:66871573-66871592 MS.gene20684:intron 60.0%
!! CCCAAGGGTTGGTTTGGTGG+TGG + chr1.2:66871576-66871595 MS.gene20684:intron 60.0%
ATCCGGGTCTTCGGCCAGGT+AGG + chr1.2:66873420-66873439 MS.gene20684:intron 65.0%
CAAGGCAGCTGCTCTGGCCA+TGG - chr1.2:66872791-66872810 None:intergenic 65.0%
CAGCTGCTCTGGCCATGGCA+GGG - chr1.2:66872786-66872805 None:intergenic 65.0%
CCGTGCAGTACGGCATGGAC+TGG - chr1.2:66871683-66871702 None:intergenic 65.0%
GACCTACCTGGCCGAAGACC+CGG - chr1.2:66873425-66873444 None:intergenic 65.0%
GGAAATCCGGGTCTTCGGCC+AGG + chr1.2:66873416-66873435 MS.gene20684:intron 65.0%
GAAGGGGGTAGGCCCTGCCA+TGG + chr1.2:66872771-66872790 MS.gene20684:CDS 70.0%
GCAGCTGCTCTGGCCATGGC+AGG - chr1.2:66872787-66872806 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 66870918 66874447 66870918 ID=MS.gene20684
chr1.2 mRNA 66870918 66874447 66870918 ID=MS.gene20684.t1;Parent=MS.gene20684
chr1.2 exon 66870918 66870942 66870918 ID=MS.gene20684.t1.exon1;Parent=MS.gene20684.t1
chr1.2 CDS 66870918 66870942 66870918 ID=cds.MS.gene20684.t1;Parent=MS.gene20684.t1
chr1.2 exon 66871057 66871161 66871057 ID=MS.gene20684.t1.exon2;Parent=MS.gene20684.t1
chr1.2 CDS 66871057 66871161 66871057 ID=cds.MS.gene20684.t1;Parent=MS.gene20684.t1
chr1.2 exon 66872217 66872278 66872217 ID=MS.gene20684.t1.exon3;Parent=MS.gene20684.t1
chr1.2 CDS 66872217 66872278 66872217 ID=cds.MS.gene20684.t1;Parent=MS.gene20684.t1
chr1.2 exon 66872365 66872601 66872365 ID=MS.gene20684.t1.exon4;Parent=MS.gene20684.t1
chr1.2 CDS 66872365 66872601 66872365 ID=cds.MS.gene20684.t1;Parent=MS.gene20684.t1
chr1.2 exon 66872679 66872849 66872679 ID=MS.gene20684.t1.exon5;Parent=MS.gene20684.t1
chr1.2 CDS 66872679 66872849 66872679 ID=cds.MS.gene20684.t1;Parent=MS.gene20684.t1
chr1.2 exon 66872956 66873005 66872956 ID=MS.gene20684.t1.exon6;Parent=MS.gene20684.t1
chr1.2 CDS 66872956 66873005 66872956 ID=cds.MS.gene20684.t1;Parent=MS.gene20684.t1
chr1.2 exon 66873794 66873926 66873794 ID=MS.gene20684.t1.exon7;Parent=MS.gene20684.t1
chr1.2 CDS 66873794 66873926 66873794 ID=cds.MS.gene20684.t1;Parent=MS.gene20684.t1
chr1.2 exon 66874216 66874253 66874216 ID=MS.gene20684.t1.exon8;Parent=MS.gene20684.t1
chr1.2 CDS 66874216 66874253 66874216 ID=cds.MS.gene20684.t1;Parent=MS.gene20684.t1
chr1.2 exon 66874351 66874447 66874351 ID=MS.gene20684.t1.exon9;Parent=MS.gene20684.t1
chr1.2 CDS 66874351 66874447 66874351 ID=cds.MS.gene20684.t1;Parent=MS.gene20684.t1
Gene Sequence

>MS.gene20684

ATGGCTGGGAATTCCTCACTTCCAGGTTACAACTTTGTTTCTTCTATTTCTCTCTAACATGTTAACAATGCATTATCACCATCACAAGTTGTAATTGTAATATTATATTGATGAAGATGAAAAATAATGAATGTATCAGGAGATGAGAAGCAGAATTGGGCCAACTCCCCTTCCACTGTTTTCAATCACTTTGCAACAAGTGGACTTTCTGTCGCAGTTGCAACATCAATTACTCACCCTCTAGGTTCGTTCTTGTTTTTCTCTTTATAATAATAATATGAACGTGATATCAAATGACCAATTTGATTGATGTCTGGTATAATATATATATATATAGTTAGGGATATATTTTGAGACTCAATTCAATTGTACTGTACTATTCAATACTGCTACTCATTAACAAAGTGTTAGACTATATCTAGAGGTTGGATTTGGTTTGGATGGGAGGGGAAGGAAAAGAAACAATTTTGTTTACCATAAACAATTTCAGTTAATTGAAAACAGAAATAGGATGCAAATGTTGTTTGACTAGCACAGAGGCATTCCCCTCCCAAGTCGTAGATCCAACAGAAACAAATAACTGATCTAAATCATGAGCTATCAAGTGCTTAACAGTTGATGGAAAACAAATACAGTTACTACTAGTACTACCATTACCCCCAAGGGTTGGTTTGGTGGTGGTATAGGCTTGAGACCTCCAAGTGTGCTCATCTCTAAGTCTCATGTTCGATTCTTCCAGGTGCTAACAATTTATGTGTTAGGCCAGTCCATGCCGTACTGCACGGTTGGATTGGTCCCAAAGATTAATCGGTCCTTGGTCTGGATACCAGGTATTTTTTTTTTAAAAAAAGTAATACTTTAGAGTAAACCCTCAATTGGGCCCTCTAAAGATCAAAGTGTTGCCGTGTCAGTTCTTGATTTTTGCCATCAAATTAGTCCATTAAAGATACATTATTGTCTCCAAATTTGTCCTTATGGTGGCCTAAAGTGATGGCAAATGAATTCTGTGATGATTTATTGATACATCGAGGATAGATCTTTTAGGTAGTTATTTTATTCTTATTCTTTCTGGGTAGTCACACTTCACATGTGGGCAGGGTAAATTGGAATTTTATTGAACTAACAGATCAAGTCAGCAGCTGTAGTAATTTAATAAAGATGTTATCATTAAGTTGGTGAAGTTCACATTGAGACCTTTAATAATATGTTGAGAAGAAACTATTTTATTTGGGTTTTGCAAAAGTAATTGACAAATCCTTAAAAATATCTATATTTAATTTGTTTCTACATTCCGTGCAGATGTCTTGAAAGTGAGGCTACAAATGCAACTTGTTGGTCAGAAGGGTCCTTTAAGTGGAATGGTAGTTCACTGAGTTCAACCTTTCCTCTTTAGACCTTACCTATTACATTATAATACAAAACTTTTGTCAATTAAGCATTTTTCCAGGGACAATTGTTTTTAAGTGTGATGAAAAATGAAGGACCTAAGTCTTTATATCTGGGTTTGACACCTGCTTTAACAAGGTCAGTTGTTTATGGAGGACTACGCTTGGGTTTGTACGAACCCTCTAAGCATGCATGTGATCTTGCTTTTGGTTCTTCCAATGTTTTAGTTAAAATTGCTTCGGGAATGTTTGCTGGTGCTTTTGCAACTGCATTCACCAATCCAATGGAAGTTCTCAAGGTGAGCCATCATATCTTTTACTTGTATATTAAACAATTTTTTTTGTTTCATCTTCAAAACCATATTCCCGCAATCAGGTGCGTTTGCAAATGAATTCAGATATGGGAAAGGGTGGACCAATTTTAGAGCTCCGGAGGACTATATCTGAAGAGGGAGTCAAAGCTCTGTGGAAGGGGGTAGGCCCTGCCATGGCCAGAGCAGCTGCCTTGACTGCATCTCAGCTGGCTACTTATGATGAAACCAAGCAGGTCAAGTTCTAATGTGTATTTTTGTTCATCTATTCCTCATCTCCCTAATTTACTTTCAATTAAACCATTGTTTTTCTAAATTTGTTTTGCTTTCCTGCTCATGCAGATTCTAGTCAAGTGGACATCTCTCAGAGAAGGATTTCATCTACATCTTATGTATGGCTCTCTCGTCATTTTTATTTCTCCGTATCTCTCTTAATGCAGACTGAACTAATGAAGTATCATTAAAGCCCAAAGATTGACCAAAATCACGGGCAACAACTTCACCATATAGTGAAGAATAATGAAGATTGACAAATCTTGAAAAATGAAATTTGCATAACTAATGTTTGTTTTATAATTGTTTTTGCGTGCATACAATATATTGAAACTGAAGTGCATATTTTCTTACAAAACTTGTTTTGTTTGTCTAATTAATTGAAACAATGATTTTCAACATTGGCACGCCTTAGGTCTATTAGTTGAGGGAGATAAAGTTAGATAGTTAGTTTGACATTAGTTGGGAACTTGGGACTAAGATAGTCTAAATGCAGGCAATGTTATATGAAACAATGTCAGGAGATGAGGGAAATCCGGGTCTTCGGCCAGGTAGGTCATTGTAACCGTGAAGAATTTGTGTTCTTATCTGTAGTCAGGGAAATCCTTCCTTGTGCTTTCTAATAAAATACAACAAGGGAAACCTTATTGTAACAGTTAGAATTGTTGCTAGGAGACTAGGTCACGGATCTAAGTCGTGACAATAGCTTATTTCAAATGTAAGATTAGGCAGCCAGCCTGCAATGAATTCTAAATGGATCCGACCAATCCTTTGTCAGCATCAGATCACCTATGTTTTCTATCAGCACACACTCTGAATTCGTTAGAATTATTACTATTTTGAAACCAAAACTATTTTCCATCATCAGAAATGTGACTTAGCTAATTTCGTTATGTACCTTATACAGCTCGAGCACAGTTGCAGGCATTCTGAGCACCCTTATAACTGCTCCCATGGATATGGTGAAAACACGTCTCATGTTGCAACGAGAATCCAAGGGTGGTAGAATATATAAAAATGGTTTTAACTGTGCATATCAGGTACTAAATTGTTTTCTCACCTCTCAAACCTTCAGTACCTTGTTTGTTCTATACCGATCTCTGCTTCTTTATTTTACGTGGTTGACTTTAGTTCATTACTTTGTAATGTGCCTTATAAGTTCTATAATTTTTTCTTCTTTAACTTTGACTAGGCTTTCATAATTCTTAATTATTAGCTTTTAATGGAATGATGGTGAAACGACTTCTGTAAAACCCAATCATGAAAAACAATGATATATTTTCTGATCTGGATTCACTTATTAAGGGCATTTTTCGTGTGTGGTTTCAGGTCCTACTTACGGAGGGTCCAAGAGCACTTTACAAAGGGTGAGTTTTATGTTTGGTATGCATTTTTACACGGAGCTTGTTTGTTTTATTCATCCTAAACTTTAATACTAATCAACATCTGATGTACATTTGTCAGGGGATTCACAATATTTGCAAGATTAGGACCACAAACCACAATTACATTTATATTATGTGAGGAGCTAAGGAAAATTGCTGGCTTGGAGGCAATTTAG
Protein sequence

>MS.gene20684.t1

MAGNSSLPGDEKQNWANSPSTVFNHFATSGLSVAVATSITHPLDVLKVRLQMQLVGQKGPLSGMGQLFLSVMKNEGPKSLYLGLTPALTRSVVYGGLRLGLYEPSKHACDLAFGSSNVLVKIASGMFAGAFATAFTNPMEVLKVRLQMNSDMGKGGPILELRRTISEEGVKALWKGVGPAMARAAALTASQLATYDETKQILVKWTSLREGFHLHLISSTVAGILSTLITAPMDMVKTRLMLQRESKGGRIYKNGFNCAYQVLLTEGPRALYKGGFTIFARLGPQTTITFILCEELRKIAGLEAI